LOCUS       NZ_CP035440            12311 bp    DNA     circular CON 15-OCT-2024
DEFINITION  Streptococcus pyogenes strain emm124 chromosome, complete genome.
ACCESSION   NZ_CP035440
VERSION     NZ_CP035440.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1790668)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1790668)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005161825.1-RS_2024_10_13
            Annotation Date                   :: 10/13/2024 02:09:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,746
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,595
            CDSs (with protein)               :: 1,595
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 36 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 16 of 62
            Pseudo Genes (multiple problems)  :: 18 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:05:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1379513
            Orig. end                         :: >1391824
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035440.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..>12311
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..>12311
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..>12311
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:>7311]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..>7311
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            23..286
                     /locus_tag="ETT59_RS09040"
     CDS             23..286
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ETT59_RS09040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_186794721.1"
                     /transl_table=11
                     /translation="MDNLSLKNNPLWENPYIQFVTGKLINIKKSVSDLAAALLLSAFAA
                     IDSKVKADETAGAYRYDVSEQNTYYQTISSINQQRPGATLDE"
     gene            180..422
                     /locus_tag="ETT59_RS09275"
                     /old_locus_tag="ETT59_06905"
     CDS             180..422
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="ETT59_RS09275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06905"
                     /product="hypothetical protein"
                     /protein_id="WP_002985713.1"
                     /transl_table=11
                     /translation="MKQQGHTVMMCLNKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLH
                     LLPKVVPVLIHLVDKANVSWLTVILQLPILLKTIW"
     gene            944..1213
                     /gene="stcA"
                     /locus_tag="ETT59_RS06915"
                     /old_locus_tag="ETT59_06910"
     CDS             944..1213
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="ETT59_RS06915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06910"
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_053308562.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHSK
                     QTPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(1561..1959)
                     /locus_tag="ETT59_RS06920"
                     /old_locus_tag="ETT59_06915"
     CDS             complement(1561..1959)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /locus_tag="ETT59_RS06920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06915"
                     /product="hypothetical protein"
                     /protein_id="WP_011054254.1"
                     /transl_table=11
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLED
                     DAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTGIT
                     YGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(2052..3121)
                     /locus_tag="ETT59_RS06925"
                     /old_locus_tag="ETT59_06920"
                     /pseudo=""
     CDS             complement(2052..3121)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="ETT59_RS06925"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06920"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGVG
                     YKEETTSQFSSDQPSQVVLYRGEANTVRFTYTDQMSLMKDIRIALDGSGKSLTAQTVPG
                     MGHVYEGLQTSARGIFTMSGVPESTVPVANPNAQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGAFPDNIKVSIN
                     SKGGRPRLLMGMALRILFLQQFYGKKAP"
     gene            complement(3372..3773)
                     /locus_tag="ETT59_RS06930"
                     /old_locus_tag="ETT59_06925"
     CDS             complement(3372..3773)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /locus_tag="ETT59_RS06930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06925"
                     /product="hypothetical protein"
                     /protein_id="WP_053308564.1"
                     /transl_table=11
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLAWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVSIYFYLHL
                     IVVCYTIGMILSLGRLISPNHSIFNKLKK"
     gene            complement(3989..4249)
                     /locus_tag="ETT59_RS06935"
                     /old_locus_tag="ETT59_06930"
     CDS             complement(3989..4249)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560515.1"
                     /locus_tag="ETT59_RS06935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06930"
                     /product="hypothetical protein"
                     /protein_id="WP_012560515.1"
                     /transl_table=11
                     /translation="MKILDIIFNLSDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRHP
                     YPIWAAVLGGISLIVIFIYFILIVYLYLRYRYLVWKKNTRN"
     gene            complement(4788..4988)
                     /locus_tag="ETT59_RS06950"
                     /old_locus_tag="ETT59_06945"
     CDS             complement(4788..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="ETT59_RS06950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06945"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_003060803.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LTGGCVVGFTVGGAYLGYTAN"
     gene            complement(5001..5228)
                     /locus_tag="ETT59_RS06955"
                     /old_locus_tag="ETT59_06950"
     CDS             complement(5001..5228)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="ETT59_RS06955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06950"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_111713896.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.4e-15,
                     bitscore: 52.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLAKVGGGKNNWQANVSGVIAAGSAGAAIG
                     FPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            5515..>7311
                     /locus_tag="ETT59_RS06960"
                     /old_locus_tag="ETT59_06955"
                     /pseudo=""
     CDS             5515..>7311
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205155.1"
                     /locus_tag="ETT59_RS06960"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06955"
                     /product="peptide cleavage/export ABC transporter"
                     /pseudo=""
                     /sec_met_domain="Peptidase_C39 (E-value: 1e-43, bitscore:
                     145.0, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 3.7e-274, bitscore:
                     908.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISYRKTFVAQIDARDCGVAALASIAKYYGSDYSLAHLRELAKTN
                     KEGTTALGIVKAAKLMGFETRAIQADMTLFDIEDVPYPFIVHVNKEGKFQHYYVVYQNK
                     KNYLIIGDPDPTVKVTKMTKERFTSEWTGVAIFLAPEPSYKPHKDKKNGLISFLPLIFK
                     QRSLIFYIILASLLVTLINIVGSYYLQGILDDYIPNQLKSTLGIISIGLIITYILKQMM
                     SFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANAIIDALAS
                     TILSLFLDVSILTIVGTVLLVQNTNLFLLSLVSVPLYIVIIFIFMHPFEKMNNDVMQSN
                     SMVSSAIIEDINGIETIKSLTNEENRYQKIDSEFVDYLDKSFKLSKYSILQSSLKQGAQ
                     LILNVIVLWFGAKLVMGGKISVGQLITFNTLLSYFTNPLENIINLQTKLESAKVANNRL
                     NEVYLVKSEFQKEQALTDTYCLYGDIQFADLSYKYGFGRDTLSDISLTIKEGEKVSIVG
                     ISGSGKTTLAKMIVNFFEPYKGQISINHQNLKMIDKKVLRQHINYLPQQAHIFSGSILE
                     NLTLGANHSTSQEDILINIRNDI"
     gene            complement(7508..7726)
                     /locus_tag="ETT59_RS09195"
     CDS             complement(7508..7726)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016296.5"
                     /locus_tag="ETT59_RS09195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="WP_072135532.1"
                     /transl_table=11
                     /translation="MVLYSTRERIEFYGELSEIVKQEPRLFQCHRSFVVNPYNISSIQR
                     LERKVYLKKRFILSSIKAKSYCSKRSS"
     gene            8027..8281
                     /locus_tag="ETT59_RS09200"
                     /old_locus_tag="ETT59_06965"
     CDS             8027..8281
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="ETT59_RS09200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06965"
                     /product="hypothetical protein"
                     /protein_id="WP_136306026.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSEPVYDLLNEIRMHYPHAHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHKSLRSIRDQIQLYLDELYNSNNG"
     gene            complement(8587..9063)
                     /locus_tag="ETT59_RS06975"
                     /old_locus_tag="ETT59_06970"
     CDS             complement(8587..9063)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="ETT59_RS06975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06970"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002985741.1"
                     /transl_table=11
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKTW
                     AIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFIYE
                     LTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     misc_feature    9020..9036
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.42"
                     /tool="antismash"
     gene            complement(9083..9979)
                     /gene="era"
                     /locus_tag="ETT59_RS06980"
                     /old_locus_tag="ETT59_06975"
     CDS             complement(9083..9979)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="ETT59_RS06980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06975"
                     /product="GTPase Era"
                     /protein_id="WP_002985743.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     misc_feature    10032..10047
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            complement(10099..10506)
                     /locus_tag="ETT59_RS06985"
                     /old_locus_tag="ETT59_06980"
     CDS             complement(10099..10506)
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="ETT59_RS06985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06980"
                     /product="diacylglycerol kinase family protein"
                     /protein_id="WP_002985746.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            complement(10487..10984)
                     /gene="ybeY"
                     /locus_tag="ETT59_RS06990"
                     /old_locus_tag="ETT59_06985"
     CDS             complement(10487..10984)
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="ETT59_RS06990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06985"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_002985748.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     misc_feature    complement(10998..11012)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            complement(11143..11718)
                     /locus_tag="ETT59_RS06995"
                     /old_locus_tag="ETT59_06990"
     CDS             complement(11143..11718)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="ETT59_RS06995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_06990"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_009880369.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
ORIGIN
        1 atggatatcc tgattatcgt tggtggataa cctttcgctt aaaaacaatc cactttggga
       61 aaacccttat attcagtttg taacaggaaa actaataaat attaaaaaga gtgttagcga
      121 cctagcggca gctttactct taagtgcgtt tgctgccatt gacagcaagg tcaaagctga
      181 tgaaacagca ggggcatacc gttatgatgt gtctgaacaa aacacttact atcaaactat
      241 cagctccatt aaccagcaac gacctggtgc cactctcgac gaatgaggtg attattatgg
      301 aacccttact tcatttactt cccaaagtcg taccagtact tatacacttg gtagacaaag
      361 ccaacgtatc atggttaaca gtgatattac agttacctat actcttaaag acaatttggt
      421 agcccttcat aaggaatata aaacctttgt caaaaaaata ttacagataa accgatcacc
      481 agtgactacg acaaggctaa ggttatttat aaccatttaa tcaacgaata cacttacgcc
      541 acaaaataac ttagcaccaa tcgcaaaacc gtaagcagca tcaacaatca tactcctaaa
      601 gctctttatc gtggcaagtg tggtgtttgt caagcttatg ccgtcatgtt taaagatatg
      661 gcagaagctg caggcttatc agcttggtat gtgataaaaa aggattgaat gagcaccatg
      721 cttgaaacat cgttaccatt catggtgtta attcctacgt tgatgctacc tggaatgatt
      781 cagcatacac cacaaggtat ttccttgctg gaaaagacgt gaggaatact gagtaccgtt
      841 tagatagcca gtacaaccac ttggcttatg ctattttaaa taccagctac caaaaaggct
      901 aaactaacaa caactacatt tttcaactag aaaggcctaa acaatgacca aaaaacagtt
      961 ttatcttatc ttagctatta gcatactgtg ttttattctt gccgtcagcc ctaaaccacc
     1021 aattcctaaa cacactattg aaaggaaaag gactgcgatg accataggcc actcaaaaca
     1081 aacccctcac aaaaaagaag cccttaaaca caaaaaaaac aaaaataagg ctctatttct
     1141 tccaagtggg gacagcgcta tcaaatctgt tattagcatg acagttagac tcctgttaga
     1201 tgaccaatct tagctctcct aaaatcagtg gacactacca tttcggtagt gttctttttt
     1261 aagctaatct catcaccacg ctttcttcaa gctaaaaaac tgatcatgaa agagcaaccc
     1321 catcacccta acagcccact ttgcccgcac tttcttaaaa agttaggaga aaacgatata
     1381 ccaattattt tctgatccca gctccctatc attaacaaga tgtgtagcac ggacctacat
     1441 acaggtaagc tagagtcatt ctcgctattt cggacaaaaa gaggccggta gttccctgac
     1501 ctcttcttta tttatcttgt aacactcctc ttcttagcga gacttactag cacataattc
     1561 ttaagcactc ttatcattta gactaggaat aacaccatct gaatgaagca aatgatgcgt
     1621 cagatctgcc agagtatcat ctccataagt aatccctgtt tggtgctgtt tctgatgaaa
     1681 atcagatgac tccttaactt tagcagtcac ctgctggtca cttggatgaa aattaatttc
     1741 tctcttggct tcagccgcaa ctggctcgag gaacaactta ttgacattac ggtcttcctc
     1801 caaatgatta aggatagcag catcatcttc taacgaaaga accttcttgg gtaacaaaaa
     1861 ggggttattt agccttggct cagtaacgac ttccttggct actcgtgata gtttattatt
     1921 gtcttcctca ggtatcaaac ggttaggcaa tcgatgcaat tcttccggct atgaggcgct
     1981 cacagctact tcagaagaaa cctccaagcc ctttcctgtt tgcctgtcta aagcaaccgg
     2041 caaattatta actaactttt taacctgtcc tcagcgctca tttcaacact aggaagagct
     2101 gtcgctggtt ctagccagct accaacttct gctggtctta ccttaacatc gctggcaggc
     2161 ttctccttct catcttcatt aggtctaaaa ggctcaaacc gccctgttga tttttcatct
     2221 atgaccttta tattgtcttt tactacatga gacaggggtt cgacatttac catatcttca
     2281 tgctcgttaa ggctctggtc tcgtttgaat ttaccaggaa taccaggagt cggtgttccg
     2341 acagattgat cggcttcagt aggctctttt acggagcctt ttttccataa aactgctgga
     2401 ggaataatat ccgtagagcc atccccataa gtaatcgtgg cctgcccccc tttgaattaa
     2461 tagatacttt aatattgtct gggaaagccc cactacctcc tgtggttaaa agggcacccg
     2521 catttttaat gagagttgtc attcgttcaa taccagcaac gccaatatgg ttcaagtcat
     2581 ctactggtaa ctgatcaaca gatttcatgg tgtatttcca agcttgcggt tgaacaagaa
     2641 aaacagttcc tttatacatt gtgttatgca tatcatcaat gactttgaaa taccttgtat
     2701 atttggtttg tgcattaggg ttagcaacgg gaacagtgct ttcaggaact cctgacatcg
     2761 taaaaatccc tctagcagaa gtttgaaggc cctcataaac atgacccata ccaggaacag
     2821 tctgagcggt caaagactta ccagaaccat ccaaagcaat gcgaatatct ttcatcagag
     2881 acatctgatc ggtataggta aacctaaccg tgttagcctc acctcgatac aaaaccactt
     2941 gggagggctg atccgaagaa aactgagaag ttgtttcttc cttatagcca actccaaaac
     3001 gtctatcaga cgaatggact gaatctgccg caactgtact actcaaactg actagtaaag
     3061 tagccgcaca accaactaat aaactgctcg ttttaacttt aacatctctt aacatcatca
     3121 tgttcctttc attatttttt atttaggaat tcgtcatact atattttctc atattagtct
     3181 aacaaaaaac tcgtaattct atataaatca tataaaatga cgagctttgg caaagatgag
     3241 tatagcagga tggaaaacag attgtttctg aaacttcaaa ggtatcttct tgtcacgaac
     3301 tattctttaa ttgaatcggt tacactaaac tagtcccatt atttaaaaac aaactttaaa
     3361 tatgattcat ctcacttttt aagtttatta aatatagagt gattaggaga aataagacga
     3421 cctaatgata aaatcattcc tattgtatag caaacaacaa taaggtgtaa ataaaagtaa
     3481 attgatacct ctgatatact cgtcaaacat cctattatta taataaaagc aattaaagta
     3541 ggaataagaa ttgtacactt aataaataac caaaattgct taacaacttg ataccatgcg
     3601 agcacttctg tcgtttgtct ttgtttgtag acaaaaacga ataaatcaag gataaaactc
     3661 caacagataa gttgtatatt tttcattaat ggtaaatcgt agaaactacc tatactaaat
     3721 agaatcactg cagaaataat aacaatacct aaaaatctta atatattacg catatcctca
     3781 cctcctctaa tttatcggta gcaaatataa ctgaacaaat tgttattcct gttatcagag
     3841 acagttataa cttttttaac caattttcaa tacgcatttt gcttttaact ctttagtctc
     3901 taaagttaaa tatcataagt actgatattg tataagtcat ccattttccc cttaaatgtg
     3961 attgcattat agctcagtaa aattaatctt aatttctggt attttttttc cataccaagt
     4021 atctataacg caaatataga taaacaataa gtataaaata aataaagatc acaataaggg
     4081 atatacctcc tagtacagca gcccaaatag gataagggtg cctaaagttg taggccacac
     4141 tcaagatgat aagggtaaaa gggacaatgt aacctcccag cttttcaaca ttttctagaa
     4201 atatcatctt tctatcagat aggttaaaaa taatatctaa aattttcata ccactcctct
     4261 actacttata catcagtgat agatagtcat ttatatattt agtgaggcca gataatattt
     4321 gtagcagcac cagctgctcc acacaaaata taaccttgcc aagggataaa tacgcctgtc
     4381 tgagcacaaa ctatcactcc ttccgcagca cctgccatcc ctgtcaaaaa gatatttgct
     4441 ccgttttttc cgcctccatt tatgatagcc agaccatttt tttagcactt ccatattttt
     4501 gaagtctttt tcattttaat ttcactcttt tgtattttaa aaagctaaga gtgatatcta
     4561 aattcattgg cataatcact attttctttt atcgataaaa acaaataatg acgctaccaa
     4621 agctacgata gataacatgt ttaccgattt ttccgaaagg gataattgat tgaaaattaa
     4681 aaacactaaa tatggtatag atttatcaac atttttttat gaatggcatc aacaatgatt
     4741 aaaaacatta tagacgcatg cataataata aaaatcgtat agatcattta atttgctgta
     4801 tatcccaaat atgctccacc aactgtaaat cctacaacac atccacctgt caatactgga
     4861 tgtggcagtt tacaaaattt caatccagaa gctaagcctc caagaccacc aattacagca
     4921 tctcctggag cgtttcctcc acttatacta gccatgtcat taattgttaa ttccttcatt
     4981 gttgtcatcc tttctttcct ctagaatccg cctgtagctc ctgttacacc agcccaaaga
     5041 gtaatagctg ttttagctcc aatataacca caggctacac cgcaaactgg aaaacctata
     5101 gcagctccag cactacctgc agcgataacc ccactgacat ttgcctgcca attattctta
     5161 ccacccccaa ccttggcaag cttttctata gtcatttggt gaaatgtttc cagtttcttt
     5221 atctccatat cgaataactt cttttcataa ttcttaaatt tgtctcgttg caacctcgac
     5281 tagtacagaa tatcataaag aacagtatca tggtttcaaa atcttaaaag gccgtttttt
     5341 catcatgaaa tgtcttttta tcctctctac acttacgctg tgacaatagt taattaatta
     5401 agaagtttaa ataataccat tcatgctttt ataaatacct tttaagattt ttgataaaaa
     5461 ctccctatat atatgtcata atagcttagt atttaataga aaggtctttt acaaatgata
     5521 tcctatcgaa aaacatttgt tgctcagatt gatgctagag actgtggtgt ggctgctctt
     5581 gcttccatcg ctaaatatta cggttcagac tactctttag ctcatttaag agagttagct
     5641 aaaacaaata aagagggaac aacagcgctt ggcatcgtca aagctgccaa attaatggga
     5701 tttgaaacaa gagctattca agcagacatg accctctttg atatagaaga tgtcccttat
     5761 ccttttattg ttcatgttaa taaagaaggt aaattccaac actactatgt cgtctatcaa
     5821 aacaaaaaaa attatctgat aatcggcgac cctgacccga cagtaaaagt cactaagatg
     5881 acaaaggagc gatttacttc tgaatggacg ggtgtcgcta tttttctagc acccgagccc
     5941 agctacaagc ctcataagga caaaaagaac ggtctaataa gctttttacc tctcattttt
     6001 aaacagcgat ctctgatttt ctatatcata ttagctagcc tactcgttac tctgattaat
     6061 attgtaggtt cttattacct acaaggtata ttagatgact atattccaaa tcaactgaaa
     6121 tcaacactcg gcattatttc aattggtctt atcattacct atatcctcaa acagatgatg
     6181 agtttttcgc gagattatct cctgactgta ctgagtcagc gattaagcat tgacgtcatt
     6241 ttatcttaca ttcgccatat ttttgagctt cccatgtctt tttttgcaac tcgcagaaca
     6301 ggagaaatca tttcacggtt tacagatgct aatgctatta ttgatgcact cgcttctact
     6361 attttgtctc tttttcttga tgtcagtatt ttaactatcg tagggacagt cctgttagtg
     6421 caaaatacca atctttttct tctatctctg gtttcagtcc ctctatatat tgttattatc
     6481 tttatattta tgcacccatt tgaaaagatg aataatgatg tcatgcagag taactctatg
     6541 gttagctcag ccattatcga agatatcaat ggcattgaaa ctattaaatc actgaccaat
     6601 gaagaaaatc gttatcagaa aattgatagt gaatttgtgg actatctgga taaatctttc
     6661 aagttaagta agtactctat tttacaaagc agtctcaaac aaggggcgca actaatcctt
     6721 aacgtgatag tcttatggtt tggggccaaa ttagttatgg gaggaaaaat ttctgttggt
     6781 cagttgatta cgtttaatac tctcttatct tattttacca atcctttaga aaatatcatt
     6841 aatcttcaaa ctaaactgga atccgctaag gtagctaata atcgtctcaa tgaagtttac
     6901 ctggttaaat ctgagttcca aaaagaacag gcattaacag atacctattg tctatatgga
     6961 gatatccaat ttgcagactt gtcttataaa tacggctttg gtcgtgatac cttatctgat
     7021 atcagtttga ccattaagga gggtgaaaaa gtcagtatag ttggtattag tggttctggc
     7081 aagacaacat tagctaaaat gattgttaat ttctttgaac cttacaaggg acagatttct
     7141 attaatcatc aaaatcttaa aatgattgat aaaaaagtgc ttcgtcagca tatcaactat
     7201 ctcccccaac aagctcatat ctttagtgga tctatcttag aaaacttaac cctaggtgcc
     7261 aatcactcca ctagtcaaga ggacattctg ataaatatca gaaacgatat tcatatcttg
     7321 aaattcgata cctatttcca aactgggcag aatataaaaa aagaaaagcg tataaagcga
     7381 aagatttttc acggttactt tatatacata tactttagca tgtaaaatta tcaaaataat
     7441 gacatgagta aaagtaagtg ctagaaagaa gatgctactc atctaagtct ccttattatg
     7501 tagattttta actgcttctt ttagagcagt aacttttagc cttgatacta gacaagatga
     7561 accgtttttt taaataaacc ttacgttcta atcgttgtat tgaagatata ttgtagggat
     7621 taacaacaaa tgatcgatgg cattgaaata atcttggttc ttgcttaacg atttcagata
     7681 actccccata aaattcaatc ctttctctag tagaataaag aaccaattta tgagcaatat
     7741 atgacgtttc aatataaagt aagtctgcaa atggaatctg aatggaatct gaacctgagt
     7801 cttggcatct ttaaaaataa aggagtcttc agctaacgtt tgaccttgtt gatgatggac
     7861 atagtcaatt gattgttcaa tacgtggaga aaattatgat agttattcaa gtcttacatc
     7921 gttctctttt aggatatatg atatctatct atattaacta ttacctcaag agatactttc
     7981 taaaaactca aattattagt gggaaaaact tagattataa atacaaatgt caggtttatt
     8041 ttatcataaa tcatactttt ttaaatctga gccagtatat gaccttctaa acgaaatacg
     8101 tatgcattat cctcatgctc atattattct aatcggaaat catattaatt acgaggaaat
     8161 atttaaaaat cattatcgag tttttggtgt tattgacaca acaagtcata aatcattaag
     8221 gtccattcga gaccaaattc aactttactt agacgaactt tacaattcga ataatggata
     8281 ggcacttcat caattctttt aggaaatgat tcttgtcacc ccttccaagt ctattgccac
     8341 agttaggttc aacttgccta agtcatgcat ggctaggtgg caatccgtca taactctaaa
     8401 acaaatgttt gatgtctgct agtatcaatt ggaaagaaaa tcctgttaac atttaacaaa
     8461 attatccaga cttaatcaga aaaattggaa gcaaaaaaag agagcataag ctctcgaaac
     8521 atcgtctttc aaaaattttt ttaacaagcc aatctccaaa aatactccaa ctgtcctaga
     8581 gagttcttaa tgccccatgg caaagctatc actaaaatat tcctctaaca tcaggcgatg
     8641 tttggctgag acactctcta actctgctat ctcctcatga gagaaaaact gtaacttgag
     8701 cgtttcctca ttatgaaagt tatcaatagc catgtcagaa acagccgtta gttcatagat
     8761 aaagacaata gtctgaacag catcaccgtt gggataaact tcctcaaaat gagtgtaaac
     8821 attcagtaag cgaacagctt caacctcaat tccggtttcc tctaagaact cacgtttgca
     8881 agtctctaaa gagctctcac ccagttccat agtgccacca ggaatagccc aggttttctt
     8941 atcaccacgc aactgcatca gcaccttgcc gtcatcattg gtcaaaatcc caccagcaaa
     9001 gttaagaata atcttatcat gcccaacttt ggagcggata taagaaatgt agtcttgtgg
     9061 catatgatct cgctttacta tgttagtatt ctttctcatt atagccaaaa tcagctaaat
     9121 caagcttttt atcccgccag tttttcttga ctttgaccca cgtttctaag tagaccttgt
     9181 cacctaacat gagttcaata tcacggcgcg ccatttttcc gatttttttg agcatggctc
     9241 cttgttttcc aatgatgatg cctttttggc tatcgcgctc caccataatg gttgcacgga
     9301 tatgcacttt atcagtctct tcatctcgct tcatggattc gacaacaaca gctacggaat
     9361 gtggaacctc ttgttgcgtc aaatgcagta ccttctcacg aaccatttct gacacaagga
     9421 aacgttctgg atgatcagta atctgatctt ctggaaaata ttggaaacct tcctcaagat
     9481 tatcagttaa taacttgatc aaagttggca cattattacc ttctagggca gaaattggga
     9541 ccacttcttt aaaatccatt tggctacgga agtcatcaat ctgttccaaa agttggtctg
     9601 gatgaacctt atcaattttg ttgataacta aaataacagg aatcttagct gcttttagcc
     9661 gctcgataat catgtcgtca cccttaccac gcttttcatc ggcaggaacc atgaaaagaa
     9721 ccgtctcaac ttcacgtaaa gtactataag cagattcaac cataaaatca ccaagggcgg
     9781 tctttggttt atgaatacct ggcgtatcaa taaagacaat ttgttctgtt tctgtggtgt
     9841 aaatgcccat aattttatta cgggtggtct gagccttgtc actcatgatg gcaatttttt
     9901 gccccatgac gtgatttaaa aaggtggatt tgccaacatt tggacgacca agaatggcta
     9961 caaagcctga tttaaacata gataattacg ggccttaacg gtagggcttc agatcaagct
    10021 tgtcactaag cttaaccgtc ttgtcctttc cttattctcc tatgccatca tcacatagga
    10081 tagcccattc ctttcttttt aatgaacgaa aatattccaa attttaggaa taaaaattat
    10141 aagtcctgtc aagacagcat agccagaaat catcaaaaca gcacctgctg ccatatcctt
    10201 ggcattttta gctagcatag aaaagtgata gtcactagcc aaatcaacta cattttcaat
    10261 agcagaattg acaatttcta aggtaatgac caaaaaaatg gctagaagta aaaataacca
    10321 ttcgatggct gaaatgctaa aaaataaacc agcaacacaa gctaaacagg cagacaaaag
    10381 gtgacttctg agattgcgtt cttccttaaa agctgtgaaa acccctgtca aggcaaactc
    10441 caggcttgag gtaatggttc tgtttttcca tttccgtttg gtggtattat tgtcgtgtaa
    10501 ggccataagc agtcaaaatc tcttcctgta aggtaaacat ctctttttct tcttcaaggg
    10561 tgtagtgatc atacccatta atatgtaaga atccatgaac agctaaaaat cccatttcac
    10621 gctcaaaaga gtgcccatat tcttgggact gctcacgcgc cttgtcaata gaaataaaca
    10681 attcgccaat ataagcatca aactctgcca tcatctctgc caaactagga tcagcagcca
    10741 aatcctcttg actaaataaa ataggcgttt ctggcttata ttctagagaa atcacatctg
    10801 ttggacgatc agtatctcgg tattctaagt tgagttcatg acttctttca ttggtcacaa
    10861 aggtcacaga catttctttt tcttctttgc ccgtcttttg agcagcaaaa ttgagcaaat
    10921 caagtgtttg ctccataatc tcttgcgaaa cttgtcccgt ttcgtcaatc atctcgatat
    10981 acataaggtt aattgttagg gaagggctgc ccacctcatt tggtatgttg ggcacccatt
    11041 attatctgac agataatttc ccttatcctt tcaatcagca tttgttattt acccattata
    11101 ccacaagccc cttaaaaacg ggaatgctag gtaaatcagg gcctatttgg ttagaattgc
    11161 ctccacacgt ttttgcaaga taggtaataa atcctgctca aaccaggggt tcttagctaa
    11221 ccacaattgg tttcttggag aaggatgaac aagtggaaaa tagtctggca agtaatcctc
    11281 aaagtgacgg actgtttcag ttaaggtttt gtaagccttg ttaccaaggt aaaagtcctg
    11341 agcatagcga ccaaccaaaa tcgtcaattc aacttctggc atcaatgctc ttaatggcgg
    11401 gtgccactta gccgcaaaac cttctctagg tggtaagtca ccggattttc ccttaccagg
    11461 ataatagaaa tccataggga taatgccaaa caatcctgag tggtaaaagg tgtcattgtc
    11521 cacacctagc cagtctcgta agcggatgcc actacgatca ttccagtaaa gtttggtttc
    11581 ttgagctact attccaggag cttggccaac aataataatc ctagctgttt gaggagcatc
    11641 atataaagga aaaatacctc tttcggtata ggagagatta gcctcatctg ccataatagc
    11701 tttggttaaa tcatccatag aatcctcctt aaaaaggcca tattaaacat catcgtaaaa
    11761 gtcttagtgc tcttgatcga tcacagggta ttttgtcaag cctggctggt aatcagcttc
    11821 aacgtcacta gccttaggtt ttagtaagtc cttcatctcc tctgaggacg tttcataagc
    11881 cttgataata tcagccacaa caggatgtcg gacaacatcc ttagcagaga aatagacaaa
    11941 atcaatctgt ttaatgcctt gtaatttctg agtagcatca atcaaaccag acttgacatt
    12001 tcgagggagg tcaatctgac tcgtatctcc attgacaatc attttagaat taaaaccaag
    12061 acgggtcaaa aacatcttca tttgcatgat ggtcgtgttt tgagcctcat ctaagataac
    12121 aaaagcgtca tccagagtac gacctcgcat ataagctaac ggagcaatct caatcacatc
    12181 tcgttccatc aggcgtgtgg tttgttcctt gcctaaaatg tgataaaggg catcgtaaac
    12241 cggtctgagg taaggatcca ccttttcttt caaatcgcct ggtaaaaaac ctaggctttc
    12301 accagcttct a
//
