LOCUS       NZ_CP035440            24230 bp    DNA     circular CON 15-OCT-2024
DEFINITION  Streptococcus pyogenes strain emm124 chromosome, complete genome.
ACCESSION   NZ_CP035440
VERSION     NZ_CP035440.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1790668)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1790668)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005161825.1-RS_2024_10_13
            Annotation Date                   :: 10/13/2024 02:09:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,746
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,595
            CDSs (with protein)               :: 1,595
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 36 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 16 of 62
            Pseudo Genes (multiple problems)  :: 18 of 62
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:05:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 943608
            Orig. end                         :: 967838
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035440.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..24230
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-i"
                     /region_number="2"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..24230
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..24230
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:14230](-)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..14230)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            complement(207..998)
                     /gene="folP"
                     /locus_tag="ETT59_RS04795"
                     /old_locus_tag="ETT59_04795"
     CDS             complement(207..998)
                     /EC_number="2.5.1.15"
                     /GO_function="GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="folP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528620.1"
                     /locus_tag="ETT59_RS04795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04795"
                     /product="dihydropteroate synthase"
                     /protein_id="WP_053308345.1"
                     /transl_table=11
                     /translation="MKIGRFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQMI
                     ADGAKIIDVGGESTRPGCLFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARAAL
                     EAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRAQAA
                     LDAGVPKNNIWIDPGFGFAKSVQQNMELLKGLDRVCQLGYPVLFGISRKRVVDALLGGN
                     TKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLS"
     gene            complement(1007..1573)
                     /gene="folE"
                     /locus_tag="ETT59_RS04800"
                     /old_locus_tag="ETT59_04800"
     CDS             complement(1007..1573)
                     /EC_number="3.5.4.16"
                     /GO_function="GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA]"
                     /GO_process="GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="folE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GTP_cyclohydroI"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989871.1"
                     /locus_tag="ETT59_RS04800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04800"
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="WP_053308699.1"
                     /sec_met_domain="GTP_cyclohydroI (E-value: 7.3e-77,
                     bitscore: 253.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKMYAEMFLG
                     LGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSDGRVTGLSK
                     LARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRGIKKPGSKTI
                     TTTARGLYKESRAERQEVISLMIKD"
     gene            complement(1627..2904)
                     /locus_tag="ETT59_RS04805"
                     /old_locus_tag="ETT59_04805"
     CDS             complement(1627..2904)
                     /EC_number="6.3.2.-"
                     /GO_function="GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /locus_tag="ETT59_RS04805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04805"
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="WP_053308344.1"
                     /transl_table=11
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIV
                     GTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRIRP
                     LTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAMVVV
                     CPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGASIWE
                     WQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTPD
                     HIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILFGAIDTK
                     PIVDMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAKADDFFVI
                     TGSLYFISEIRKYWEKHIEKSVLLTH"
     gene            3235..4197
                     /locus_tag="ETT59_RS04810"
                     /old_locus_tag="ETT59_04810"
     CDS             3235..4197
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /locus_tag="ETT59_RS04810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04810"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_011017736.1"
                     /transl_table=11
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGLS
                     DVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPILMY
                     HAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKKVVW
                     LTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEMGSAANLTLKQMKEMKQFGMSFQD
                     HTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAARLNYK
                     LGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            complement(4314..5549)
                     /gene="pbp3"
                     /locus_tag="ETT59_RS04815"
                     /old_locus_tag="ETT59_04815"
     CDS             complement(4314..5549)
                     /EC_number="3.4.16.4"
                     /codon_start=1
                     /gene="pbp3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /locus_tag="ETT59_RS04815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04815"
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="WP_080370135.1"
                     /transl_table=11
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVL
                     YEKDAKMPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFD
                     KKTYSVQDLLTATLVASSNSAAIALAEKVADSELHFVNQMREQLSHWGITSGKILNASG
                     LPNEVLKDHRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGNSIKS
                     FNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKNQNTRF
                     EQANRLLDYVARTYCRRKILKKGSLVLERSLPIQDGQVKELPVSVAEDVTIILQQGEQV
                     PKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSRKKASDL
                     QLWWRDFLEKRR"
     gene            complement(5707..6894)
                     /locus_tag="ETT59_RS09255"
                     /old_locus_tag="ETT59_04820"
                     /pseudo=""
     CDS             complement(5707..6894)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050426.1"
                     /locus_tag="ETT59_RS09255"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04820"
                     /product="tyrosine-type recombinase/integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTIKNQKKYKGVYCDKNGKILYQADLGVDPVTGKRVQKKARKN"
     gene            complement(6897..7118)
                     /locus_tag="ETT59_RS04825"
                     /old_locus_tag="ETT59_04825"
     CDS             complement(6897..7118)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /locus_tag="ETT59_RS04825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04825"
                     /product="DUF3173 family protein"
                     /protein_id="WP_053308698.1"
                     /transl_table=11
                     /translation="MNIGFLEHTARNIIREAKRIAIKKFEEARELEQNAVQLSKSPFDN
                     RRLGIAPKDIVEELVGILLSEESLESEN"
     misc_feature    7132..7147
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     gene            complement(7405..7635)
                     /locus_tag="ETT59_RS04835"
                     /old_locus_tag="ETT59_04835"
     CDS             complement(7405..7635)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775551.1"
                     /locus_tag="ETT59_RS04835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04835"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_053308340.1"
                     /transl_table=11
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADVL
                     VQFSKLYNVSIDYLLGQTDYPYRFHNKKVSP"
     misc_feature    7594..7605
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.71"
                     /tool="antismash"
     gene            complement(7826..8548)
                     /locus_tag="ETT59_RS04840"
                     /old_locus_tag="ETT59_04840"
     CDS             complement(7826..8548)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /locus_tag="ETT59_RS04840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04840"
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="WP_053308339.1"
                     /transl_table=11
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQSR
                     TVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFLWLC
                     YCIVLIISITIFILLLKTIGINVSLRLLILNSLIYIIFAYVTYLINHIISYIFSTGVAL
                     GISMVGVIVAAFCETSLGDKVWFLIPWAWLLRISDTLYNQQKMAIVPLVVIFFVSCTVA
                     LLHIRIFKSWNQDCLTSS"
     gene            complement(8551..9300)
                     /locus_tag="ETT59_RS04845"
                     /old_locus_tag="ETT59_04845"
     CDS             complement(8551..9300)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /locus_tag="ETT59_RS04845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04845"
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="WP_002989889.1"
                     /transl_table=11
                     /translation="MVQIFLSNLFKIKNTSISKLILIFPIICVMLSLLFSALGGESILR
                     LAAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLIAL
                     INLIISMILSLMLIVISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKINVY
                     FAMIINIISPLIIGTSFSLLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPDYSQI
                     FISLFLGIAFFILLTNLFAISFKKQVK"
     gene            complement(9306..9995)
                     /locus_tag="ETT59_RS04850"
                     /old_locus_tag="ETT59_04850"
     CDS             complement(9306..9995)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /locus_tag="ETT59_RS04850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04850"
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="WP_019418719.1"
                     /transl_table=11
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIMK
                     ILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVSES
                     AILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIGIQE
                     LREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIVLEKEYDGSENLEELFNNQ
                     ILFEKRR"
     gene            complement(10001..12967)
                     /locus_tag="ETT59_RS04855"
                     /old_locus_tag="ETT59_04855"
     CDS             complement(10001..12967)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /locus_tag="ETT59_RS04855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04855"
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_168389180.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 4e-90, bitscore:
                     299.4, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 1.5e-11,
                     bitscore: 41.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.6e-13, bitscore:
                     47.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSLY
                     DSVIDFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKVSF
                     IKKAVPDYSWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTVNESEEDRNISFS
                     KPIKLLVEKCKTRYIKYEELIDVFKNNYPDIVSDMLESYIHDLVTNDFLISDLRPPICN
                     INSLDYLLSKLEEGTLCTDLTTLKKMIEDYNDLNIGEGRDLLLQIFDKMESIYKYDNGG
                     NYLKVDSLAEYEGGFNILSETARESIERLAEMFVKLNTNVKRRGGRKNSLEEYQLKFIG
                     KYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQMFEKKYEEALLNGGHIE
                     FYSKDLDDFQTDQSNSLDSFELNFNIKIINNDVKLYLGANIGSGQAGRSFGRFYGLSET
                     VRETIKNLNHQNNTNVELSFVPKQIRLANVIQNYSDESYNTSFFTTSWDSENELRLEDI
                     YIRYSDGKFHFTTIDGKNELNFTMNNMLNSDSQSRVLRLLVDLSEFEYGLSHWSLFPWD
                     ILAQERVYIPEILFEDITITTAQWNLSAIREEIIHQKIFTGKKDIFNKFRKTYKIPKDI
                     ILKYADNELRLDLSIDSDLEILFKNINKYRFVSLRGIEQGESIFKDNLGNYNTEIVVPI
                     LKESNGINNTNDNVQFKNEVKKIYLPFCDWLFFKFYGPKERQEELLNNWKKFWLNLPSE
                     LFSQSNMFYMRYNDTNDHIRIRINCDSIENNFSLYLSVVQDLLPQLIESCIISDIEVSS
                     YKPEVNRYGGPHLISYAEEIFCKESILFLNNTIEFSDDERLVCATYLVLYYLNHFFKDK
                     ETRRSFLFENYTGKYKKDFKNLPIDLPIEYMKILNGVTSALDRYDFFQEMDRYLMSYMK
                     EYNRFGGTNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKVYCFAYYTLNAQQYIEWE"
     aSDomain        complement(12311..12565)
                     /aSDomain="Lanthipeptide_LanB_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing LanB protein in a class I
                     lanthipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_ETT59_RS04855_Lanthipeptide_LanB_RRE.
                     1"
                     /evalue="2.60E-13"
                     /identifier="RREFam001.1"
                     /label="ETT59_RS04855"
                     /locus_tag="ETT59_RS04855"
                     /protein_end="219"
                     /protein_start="134"
                     /score="41.5"
                     /tool="antismash"
                     /translation="NTAAFTQGDRYVLPFTVNESEEDRNISFSKPIKLLVEKCKTRYIK
                     YEELIDVFKNNYPDIVSDMLESYIHDLVTNDFLISDLRPP"
     gene            complement(12986..14230)
                     /locus_tag="ETT59_RS04860"
                     /old_locus_tag="ETT59_04860"
     CDS             complement(12986..14230)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /locus_tag="ETT59_RS04860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04860"
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_002989896.1"
                     /sec_met_domain="LANC_like (E-value: 6.8e-78, bitscore:
                     258.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPQIKQIVDYIADSFINYEQIVAESENKVLINIESKYRNTFFDK
                     SLSHGVPSLILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAGTAGIAL
                     AIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVNGFSGITN
                     YLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLGIAHGITGPMLILAK
                     LKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMAWCYGT
                     PGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIYDLIGKN
                     YSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVSGIVLFLL
                     NPYSDEELLWQRIFI"
     gene            complement(14214..16004)
                     /locus_tag="ETT59_RS04865"
                     /old_locus_tag="ETT59_04865"
     CDS             complement(14214..16004)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /locus_tag="ETT59_RS04865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04865"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_011284758.1"
                     /transl_table=11
                     /translation="MIKAQLKNLIKTIHLVKEAGFSLLVFSIAISLFQGILPIFSMLLV
                     QKMLNIITTDIKNFHTLMIAFISYIALTLLTIIIGEVDSYIDTKLQILLHYKMNHLVMQ
                     KTVKLTLAEFETPEIYDDITRIQNQISYKPFQIYKSIISVLSSLVSLISSFVILLNWKI
                     SIFPLLLILPIVSIYIYLKIGKNEFEILYKRSSDERANWYISHLLTHDFAVKELRLGTL
                     ESYFLKKYDKNNQLFINQENSINRSKMKYGIVLGVLEIAIQSIIMFLAVREAFLGILLV
                     GNVTTFIRSLSTMQTSTQNIVNNIYSFYNGSLYMELLYDFTHDELVHNPIIESTVDTIQ
                     SVEFERVSFSYNGKDNVINDISFSIKAGERLAIVGENGSGKSTIFKLACGLYDNYEGNI
                     YINGINLRSIQKKSYYKRISALFQDFLKYELTLRENVGLGELSKLYSDEDLISALKYTG
                     VDSIFYFNQEKLNQIDLEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEADCYLLDEPSS
                     ALDPESETKIFKTFFELSQNKIGIFITHKPSITQSVNKILYLDNGCIAETGSFADLNIK
                     GMKFKNLLDKEIGATYDTSN"
     gene            complement(16119..16259)
                     /locus_tag="ETT59_RS04870"
                     /old_locus_tag="ETT59_04870"
     CDS             complement(16119..16259)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /locus_tag="ETT59_RS04870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04870"
                     /product="lantibiotic streptin"
                     /protein_id="WP_002984803.1"
                     /sec_met_domain="TIGR03731 (E-value: 7.8e-09, bitscore:
                     32.1, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTTV
                     K"
     misc_feature    16372..16387
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.51"
                     /tool="antismash"
     gene            complement(16416..17762)
                     /locus_tag="ETT59_RS04875"
                     /old_locus_tag="ETT59_04875"
     CDS             complement(16416..17762)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984805.1"
                     /locus_tag="ETT59_RS04875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04875"
                     /product="sensor histidine kinase KdpD"
                     /protein_id="WP_053308337.1"
                     /transl_table=11
                     /translation="MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTSH
                     NVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKTDKAMEGKYLSKEFSLFNEVAEEKN
                     NLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIVVAL
                     TRFVKEISLNFKEIKKLANKMGIEVLSEKENYSKIIEFDDILRTLHIKGDNLKSLIERE
                     ILEKQDLSFQIAALSHDIKTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYF
                     NSLISYTRMLSEDRPVKLILVEELLSELHFEVDDLLNINNIEFSICNRLIITSFYGDEE
                     NLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFY
                     TEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI"
     gene            complement(17755..18441)
                     /locus_tag="ETT59_RS04880"
                     /old_locus_tag="ETT59_04880"
     CDS             complement(17755..18441)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /locus_tag="ETT59_RS04880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04880"
                     /product="response regulator transcription factor"
                     /protein_id="WP_002984807.1"
                     /transl_table=11
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFKG
                     YDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKPFS
                     LKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYREYE
                     ILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKTIRGI
                     GYKWDV"
     gene            complement(18592..>20086)
                     /locus_tag="ETT59_RS09005"
                     /pseudo=""
     CDS             complement(18592..>20086)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294852.1"
                     /locus_tag="ETT59_RS09005"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="SAG1250 family conjugative relaxase"
                     /pseudo=""
                     /transl_table=11
                     /translation="TDKNHTHNHIQSTPLTVIQIKN"
     gene            complement(<20077..20402)
                     /gene="dcm"
                     /locus_tag="ETT59_RS04900"
                     /old_locus_tag="ETT59_04900"
                     /pseudo=""
     CDS             complement(<20077..20402)
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="dcm"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /locus_tag="ETT59_RS04900"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="ETT59_04900"
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MQFLDLFAGIGGFRLGLINQCHECIGFCEIDKFARQSYKAIYETE
                     GEIEFHDIRQVTDQDFRQLRGQVDIICGGFSLVKHFHSQADDWDLRALGELSSLRLLER
                     PNR"
     gene            complement(20577..>21176)
                     /locus_tag="ETT59_RS09010"
                     /pseudo=""
     CDS             complement(20577..>21176)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /locus_tag="ETT59_RS09010"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="replication initiator protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="AIYLIYSNSK"
     gene            complement(21435..21800)
                     /gene="rplL"
                     /locus_tag="ETT59_RS04910"
                     /old_locus_tag="ETT59_04910"
     CDS             complement(21435..21800)
                     /GO_component="GO:0005840 - ribosome [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rplL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /locus_tag="ETT59_RS04910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04910"
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="WP_002984819.1"
                     /transl_table=11
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAAG
                     GAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAAEA
                     EEIKAKLEEAGATITLK"
     gene            complement(21865..22365)
                     /gene="rplJ"
                     /locus_tag="ETT59_RS04915"
                     /old_locus_tag="ETT59_04915"
     CDS             complement(21865..22365)
                     /GO_component="GO:0015934 - large ribosomal subunit
                     [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rplJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /locus_tag="ETT59_RS04915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04915"
                     /product="50S ribosomal protein L10"
                     /protein_id="WP_002984821.1"
                     /transl_table=11
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRRS
                     LRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTKTA
                     DALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAENKE
                     GAA"
     misc_feature    complement(22394..22529)
                     /db_xref="RFAM:RF00557"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
     gene            22700..24076
                     /gene="mnmE"
                     /locus_tag="ETT59_RS04920"
                     /old_locus_tag="ETT59_04920"
     CDS             22700..24076
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="mnmE"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /locus_tag="ETT59_RS04920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT59_04920"
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="WP_002989919.1"
                     /transl_table=11
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNL
                     EQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEILQL
                     LIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLSQLI
                     NDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRGKILR
                     EGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAG
                     IRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRIILLNKT
                     DLEQKIELEQLPADLIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISL
                     IEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
                     "
ORIGIN
        1 actaactgac ggacactgtc aaacaccatc ccgtaatgga ccgtatctgt taattggtct
       61 gacaaagaag ctgcttgcaa atcgactgac aattctaaat caaccagaaa aatttgccca
      121 agggtctgtt cttctttaaa ggctccatgg tagccataaa aacggcaacc ttctaatacg
      181 attttatcca taagtcctca catcaactag ctcaaaacag ccacaatgtc ttgattagcc
      241 ttgacatcat gcacccgtac aatctgacag ccttttccaa gggcataagc agacaaggct
      301 gctgtcgctc cgtctcgctc tttagctttg gtgttgccgc ctaacaaggc atctacgaca
      361 cgctttctcg aaataccaaa caagacagga taacccaact gacagacgcg gtccaatcct
      421 tttaataact ccatattctg ttgaacagat ttggcaaatc caaatcctgg atcaatccaa
      481 atattgtttt ttggcacgcc agcatcaaga gctgcttgtg ctctattgcc tagaaaatca
      541 caaacgtctt gtgttacctc ttgataaact tcttcgtctt ggttatgcat caagatgata
      601 ggcgcatcgt actcggctgc taaggcaaac atttgaccgt cgtacaaacc tgcccaaaca
      661 tcattgagaa tatcggcacc cgcctctaaa gctgctctag ctgtttcggt tttataggta
      721 tcaatgctga ttagaatatc atagttttct ttgatggcct tgatcacagg aaccacccgg
      781 tcaatttcat cggtagcgct tacaaatagg caacctggac gtgttgattc tccaccaacg
      841 tcgatgattt tagcaccatc agcaatcatt tgctcaacgt gatctaatgc tttttgcaca
      901 gtagtgtaag accccccatc tgaaaaagaa tctggagtca catttaaaat ccccatgata
      961 gccgcattgc cctcaatcac aaaccttcca attttcataa gtcctcctaa tcttttatca
     1021 ttagagaaat aacttcttgc ctctcagcac gactttcctt gtataagcct ctggcagtgg
     1081 tggtaatggt tttactacct ggctttttga tgcctcgcat ggtcatgcac atgtgctctg
     1141 cttctaccat aactaaagtc cctttaggat tgagggcttc aactaaagca tccgctattt
     1201 gagaggtcaa acgctcttgg agttgaggtc gtttactagc tacttcaact gctcgcgcta
     1261 atttactcaa acctgttact cgaccatcac ttggcaagta agcgatatga gccttgccat
     1321 aaaaaggaac taagtggtgt tcacatatag aataaaaact aatgtctttg acaatcacca
     1381 catcctcatg ttgttcttta aagacagccg taaactcctc tttaggatcc tttcccagtc
     1441 ccaaaaacat ctccgcatac attttagcaa cccgtttagg cgtgtcaaga agaccttctc
     1501 gatttggatt ttcaccaata gcctctaaaa actgataaat ggctgcttct gctttttcct
     1561 tattaataga cattagtctt tcccttttca ttagtttttt ccttttcctt ttcattttct
     1621 ctttttttaa tgggttaaaa gaacgctttt ttcaatatgt ttttcccaat actttctaat
     1681 ttctgaaata aaatatagtg accctgtaat cacgaaaaag tcatctgctt tagcatgctt
     1741 acgcaaggcc aagaaatctt tgaaatcagc aaccctacca aaacgttctg ggtatttttc
     1801 caatggatag gcgttagggt aatgaaagct agtaacctgc aagtcaccta tttgctcaag
     1861 agcaaccaac atatctacaa taggcttggt gtcgatcgca ccaaacaaaa tatgaagctt
     1921 tttatcattg tagttatttt ttaagacggc taccaaagca gcaacgcttt cattgttatg
     1981 agctccatca atcattagat ttggtgctaa taattcggta cggcctagcc acaaactatt
     2041 tgccagacct tcccttatat gatctggagt tagcctagga tagcggtctt tcaaggttaa
     2101 gcaagtcata atagctaaag ctgcattaga cacttggtgg tgtcctggca tagcgatgtg
     2161 aatatctgaa ataactccta aaggactagt aaaacgatag ccagatgcgt tctcagccat
     2221 ttgaaactgc tcttgccatt cccagattga agcaccaacc tgctcagctt ttgttaaaaa
     2281 aacctctcga gctgacgggt tttcaacggc aaaaacaagg gtctcgcccc cttctaaaac
     2341 accagctttt tgggcggcaa tatcagcgta agtctctccc aaaatagctt ggtgatctaa
     2401 accaattgag ggacagacca caaccattgc ttgaaaaaca ttggtcgaat cataaagacc
     2461 gccaaggcct gcttcaataa tagcaatatc tacaggatgc atatcaccaa aatagaggaa
     2521 catgatcaaa gtaatcactt caaattcagt tacctctcca aaatcagtct cttgaactag
     2581 acgctctgtc aaaggacgaa tacgattagc agcaataacc aaatcttttt ctgaaatcat
     2641 tcgaccattt atgctaatac gttctttgaa atccataata taaggagagg taaaggtccc
     2701 cacttcataa ccagctgtcg taaagatgtg ttgtaaatga ttgaccgtgg aaccttttcc
     2761 gttagttcca acgatatgca ctcccttgac atttttctgg ggatttccta actgtccaag
     2821 aacccaaagc atacgtttta agcctggctt gatgccgaaa actaagtgat catgaatcca
     2881 ttctagagtc tcttcataag tcatttcata cctcacatct atctttagta taccatatat
     2941 aaaaaaaagt gacagcagat aacctagagt agtacccttt ttttattatc ctcaccaaac
     3001 actcaaataa gttctaaagt acactacact tttattttca ttgctttttt tacgtgataa
     3061 gaataccagc cccacatagt ctttgctacc tcctaaagtg ttatctgtga caatattttt
     3121 tttactcctt aatacacgtt cttccaaaaa acaagaaaaa aggctgactt caagccttct
     3181 tttctctaaa aaatgttaca atagaggtac ttatatcaaa aaaaggagca agtaatgaac
     3241 aatagacata aacggcgcca aaaaagaaaa gccttcactc tcattaacct tatactatta
     3301 tttgcctgca ttcttggttt gaccctcatt ttctatttat atcaagggtc taacaaattc
     3361 ggcttgtcag atgtctctca atttgtacct aaaataagtc tttttcatca caaaaagacg
     3421 gcaaaaaaag aaacgacaaa acttaagaag actcatttcg attcctcaaa atctcaaaaa
     3481 aaggcccatt ctaaattaac ttggaccaag caagaaacac ctgtcaagat cccaatttta
     3541 atgtatcatg ctattcatgt aatgtcccct gaagagacgg ctaatgctaa tttgattgtc
     3601 aacccagatc tctttgatca acaacttcaa aaaatgaaag acgagggcta ctattttttg
     3661 agtcctgaag aggtctaccg cgctctttca aataatgaat tgcctgctaa aaaagttgta
     3721 tggctaacat ttgatgacag tatgattgat ttctacaacg ttgcttaccc aatcttaaaa
     3781 aaatatgatg ctaaagccac caataatgtt attacaggac taaccgaaat gggaagcgct
     3841 gccaatctga ctcttaagca gatgaaagaa atgaaacaat tcggtatgtc tttccaagat
     3901 catacagtga atcatcctga tctagagcag gctagtcctg atgttcaaac aactgaaatg
     3961 aaagattcaa aggactatct tgataagcaa ttaaaccaaa acactattgc cattgcttat
     4021 ccatctggta gatataatga cactacctta caaattgcag ctagacttaa ctataaatta
     4081 ggtgttacca ctaacgaagg aatagctagc gcggctaatg gacttctttc tcttaaccgg
     4141 attcgtatcc tcccaaacat gagtccggaa aatcttttac aaacaatgga accataacat
     4201 caattttttt atcattccaa atcatactca ctgtaaacta aaaagacagt tatgaaaaaa
     4261 ttctttcata actgtctttt tttgattgtt ttaaaattat tttattctta tctttaacgt
     4321 cttttttcaa gaaaatctcg ccaccacagc tgtaagtcag aggctttttt tcgagattgg
     4381 ctagctttaa gaggaatacg cttaggttcc tttaaatacc tcactgactg atctgttatt
     4441 cttggagacg tcaaataagc aaccacttca cctttagtaa tcggtgctag taaagaagtc
     4501 tctgatatca taaattgttt aggtttggga acttgttcac cctgttgcaa aatgatggtt
     4561 acatcttcgg caacggatac cggtagttct tttacttggc catcttgaat agggagcgac
     4621 cgctccaaga caagacttcc ttttttaaga atcttacgac gacaataagt tctagccaca
     4681 tagtccaaca aacgatttgc ttgctcaaac cgcgtgtttt ggtttttatc agatccatcg
     4741 gcatttagaa taacggtaat cactcgcata ccattttcaa tagaagttac cacaagacaa
     4801 tgacctgcca agtctgttgt cccagtcttt aaaccatcca ctcctgctct acccttagcc
     4861 atacctggta agagttgatt aaaactttta atagaattgc cagcaaaatt aacttctgtt
     4921 tgtttggtga tctctaaaat ctcaggaaaa tcttctaaca aatgcatcgt cacaactgca
     4981 acatcttgag cactcaaggt attttcttct tccaacgcag aaccaggata acgatggtcc
     5041 tttaacacct catttggcag acctgaggcg ttgaggattt taccagaagt aatcccccaa
     5101 tgagatagct gttctctcat ttgatttaca aaatgaagct ctgaatccgc cactttttcg
     5161 gccaacgcaa tagcagcgct atttgagctt gctaccaaag tagctgtcaa caggtcttga
     5221 actgaataag tttttttgtc aaaaggtaca ttacttaaag acctatcttt tgttaactcg
     5281 aaggcataat ctgagagatt aacttgatct gataattgca gctggcctga tttaacctta
     5341 tctaaaacca gataagccgt caccaatttg gtcaaagagg caatagcatc tggcatctta
     5401 gcgtctttct catacagaac tctcccagag tctgcctcta cgacaatcgc atgttctgct
     5461 cccacttgaa aatcagctgc gagaactgtt ttaccaaacc ctgaacaagt taataccata
     5521 aaaaaaagga taacccacct ttttatcatt atatctttca tagttactat tatagcaaaa
     5581 attctcagaa atttggaagg aagtatagtg gcacacacct ttagtagctt aaacaagctc
     5641 ataaagcaag taccctgtaa caataacaca tcctgttata tggacgatat aataagcttc
     5701 gttatatcaa ttagttgttt ggcaggaatt ttggtataaa atggaatcta atcctgaact
     5761 tatcacattt tgggttattt cttcagatac tgctttatct aatgcattaa accagtgact
     5821 atatgtattc aaagttgtag acttatcagc atgtcccata cactttgcaa cgtacagaat
     5881 gtcttttttc aaaacattaa taagatagga agcatgacta tgtcgcaagc cttttcccgt
     5941 tatcactggt actcctactt cttgagcttt gtgcttaata agtcgataga ttgtcgattt
     6001 ataaaacggt tcaccaaact gtgaaataat gaaatctgtg tcagtattga ctacttgatt
     6061 tcttctccaa ttttccaata ctactattat cacatcatct aactcaataa gacgttctcc
     6121 tgctggcgtt tttgtttggt ctttacgata ccaatttcca ttttcatcat tttccaaggt
     6181 tgtatgaact ttcaagaatt tttgttcgaa atcgatatct tcccatcata atgataaacc
     6241 ttcacttaca cgaacacctg tcatataata tagccaaata gttgtaaatc tttgtagctc
     6301 ttcataatct tgcaaatcaa acgattttat aaaattctga aactcaatat aagtccaaaa
     6361 tggagtgtcg ggatgtttcc ctttaggatt atccaaagcc ttacacggca cttttgaaat
     6421 atagtctaac ctttcagcat accccatgca agctttgaat cgcgaccata aattctttgc
     6481 ataattttca ataatattta aactaaaaga ttcacaatct caagctgtta ttccctttaa
     6541 aagtttagaa ccaaaatact gaataaataa cttatgatgt ggtaaggcta tttcataagt
     6601 tacagcctga accttttgct tatatgctct taggtaaata gtattcatat agttttcaaa
     6661 cgatatattg tcattttcaa tactaagatt attactaaac tcatacctaa tccgaaccag
     6721 ttcatcatac gcttctttca ttgtaccaaa tggctttcca tactaattct ttctggcttt
     6781 cttttgaact cgtttacctg ttactggatc tacaccaaga tcagcttgat aaagtatctt
     6841 cccattctta tcacaataaa cacctttgta ctttttttga ttttttatag tcatctttaa
     6901 ttctcacttt ctaaactttc ttcagagagt agaataccta ctaactcttc tacaatatct
     6961 ttaggagcaa taccaagtct tctattgtca aagggcgatt tacttaattg taccgcattt
     7021 tgttcaagtt cgcgagcttc ttcaaacttt tttatagcta ttctttttgc ttctctaata
     7081 atatttcttg cagtatgttc gagaaaacca atattcatca agctttttat atgttactat
     7141 tttcacacct ccactccttt caaaaaaatt acaacctccg tgggtatgac tggttaaatc
     7201 actcatggga atctcaccca ctggcctttt acgctggcag cctgaatgtt cagaagtatc
     7261 attgtcccta tttgtggtca tggcagatag agaaccactc tattttatag ctaataatta
     7321 tcgctcctat cacggcgcat ctcactaaat tgctcgacaa acaagaaagt cccacctcgg
     7381 tttattcagt tgtcaatgaa taaatcaagg cgagactttt ttattatgaa aacgatacgg
     7441 ataatctgtt tgccctaata aataatcaat actgacgtta tagagtttag aaaactgcac
     7501 aagaacatct gccattaagg caacctctcc tctctcaatt tttgcataat ttgcatgaga
     7561 gaaggaaagt aaatttgcaa caaacttttg agtgaaatcg ttatcttctc tcaaattcct
     7621 aatacgcggg tacatctaac attctcctaa tatcagtata aaggagattt gattaaaaaa
     7681 tgtttaattg tcctatttgg acaagtctat taaatataag ctagtaaacc attgaaattt
     7741 tatagtcatt ttttagttaa acaagttaga tagtatttat aacttaaaaa tcatatttat
     7801 tgttaggtaa acaaaataga tgtatttaac tacttgtcaa acagtcttga ttccaacttt
     7861 taaatattcg tatatgaagt aaagctactg tgcatgaaac aaaaaatata acaacaagtg
     7921 gaacaattgc cattttctgt tggttatata gagtatctga tattcttaaa agccatgccc
     7981 atggaataag aaaccatacc ttatcaccaa ggctcgtttc acaaaaagct gcaacaataa
     8041 cacctaccat tgaaatacct aatgccacac ctgtactaaa tatatagcta ataatatgat
     8101 tgatcagata ggttacatag gcaaaaatga tataaattaa actattcaaa ataagtagtc
     8161 tgagagatac atttatacca atagttttca ataaaagaat aaatatagta attgaaataa
     8221 ttaatactat acaatagcat aaccatagaa atactaattc tacaataaat ggcttataat
     8281 tcgactgtga gtaacccaat aaatatttat attttccaga tgattcttcc aaatttttta
     8341 gttgaatgca cacaatactt gcaacaaaag gaagaagtac acctactatt tgaaagtagg
     8401 aaaataatac agtcctgctt tgtaaacctc cacgttgact ccaatagaaa aatagaagta
     8461 agggaaataa tagtgcagct aaaatatgta ttagtaaata tgaagtattt ttgattttta
     8521 aaaaattaga acataaatat gatttcatga ctatttcacc tgttttttga aagagatagc
     8581 aaatagattg gtcagtaaga tgaaaaaagc aattcctagg aacaatgata taaaaatctg
     8641 tgagtagtct ggaacaaact ccgctccaaa aggtatacta tttattctca ttcttgtcat
     8701 tggctcaagc aacttcaacg accaatcata agggaacaaa taccatttat ttaagagaga
     8761 aaaacttgta ccaataatta gtggagatat aatattaata atcatagcaa aatacacatt
     8821 aatttttctt gatagccata aaataaacgg gatttgccat agtgtagtca accaaattaa
     8881 caagatagtt aatagtatcc tacctatact aataagactt ggagtaggta ggacaaaact
     8941 aatcacaata agcattaagc ttaaaatcat actaattatt agatttataa gagctatcaa
     9001 tagaactctg gcatattcta ccttttttaa atcaacatcc cttgataaaa aaacagaata
     9061 cttattagaa tctaattcta atttatttaa caaaccagaa tttacagcaa taataacatt
     9121 aatccaaata actccccact gatttactgt tgtttccgca gctaaacgta ggatactttc
     9181 accacctaat gctgaaaaca ataaagataa catcacacaa ataataggaa aaataagaat
     9241 caacttgcta atactcgtat tttttatttt aaataaattt gataaaaaaa tttgtaccat
     9301 agcccctacc tcctcttttc aaataaaatt tgattattga ataactcttc aagattctca
     9361 gagccgtcat attctttttc cagaacaatc tttccttcat agataaatcc aataaaatca
     9421 gctagatgtt caacttctga cagtatatgg cttgaaatca taattgtcat tccttctgat
     9481 ttaaatgact ctataatttc ccttaattct tgaattccaa ttgggtccaa gccattagta
     9541 ggttcgtcta atataagtaa tttaggtgaa tttattaaag agatagctat acctaaccgt
     9601 tgcttcattc caagagaaaa ttgttttact ggtctcttgt caatatttcc tagaccaact
     9661 ttatttaata atggaagtat agcgctttct gaaacaccaa gcattgttgt aactaccttc
     9721 atattatcgt aagcactcaa atttttataa agtggtggtt cttcaataat actcccgatt
     9781 tttcgcagat cccgacgtga ccattctcta ccttcaaaaa taatagaacc acttgtctta
     9841 ctaactaagc ctgtcaaaat tttcataata gttgattttc cagcaccatt tggaccgatt
     9901 aaagcataca cttttccctt aggaatattc atattgacat tattcaatat ggttctctta
     9961 ccataagatt tttttagatt ttgtattttc aacatattag ttactcccat tctatatatt
    10021 gctgagcgtt tagtgtatag taagcaaaac aatacacttt ctcttcaaac tctctatcaa
    10081 tcccgtttaa tcgattcatt gatagatgta aaaaacttcc tactaaattg aatttagtat
    10141 tatagatatc atttgtacca ccaaatcgat tatactcttt catataagac atgagatatc
    10201 tatccatttc ttggaaaaaa tcatatctat caagagctga ggtgactcca ttcaaaatct
    10261 tcatatactc aataggtaaa tcaattggca aatttttgaa atctttctta tactttccgg
    10321 tataattctc aaataagaaa gagcgccttg tttctttatc tttgaaaaag tgatttagat
    10381 aatatagaac caagtaagta gcacaaacta atctttcatc atctgaaaat tctattgtat
    10441 tattaagaaa taagatagat tctttacaaa aaatttcctc tgcataactt attaaatgag
    10501 gtcctccata tctatttact tctggtttat agctactcac ttctatgtca gagataatac
    10561 aactctcaat cagttgaggt aatagatctt gaactactga caaatacaaa gaaaagttat
    10621 tttcaatcga atcacaattt atccgaattc ttatatgatc attggtatca ttatatctca
    10681 tataaaacat gttactttgg ctaaacagtt ctgatggcag gtttagccaa aattttttcc
    10741 agttatttag taattcttct tgtcgctctt ttggaccata aaatttgaaa aatagccagt
    10801 cacaaaatgg taaatatatt ttcttaactt catttttaaa ttgaacatta tcgtttgtat
    10861 tgtttattcc attagattcc ttaagaattg gaactactat ctctgtattg tagttcccta
    10921 aattatcctt aaaaatagac tctccctgct caattcctct caaagaaaca aacctatatt
    10981 tattaatgtt tttaaataaa atctctaaat cactatctat agataaatct aacctcaatt
    11041 cattatctgc atatttcaaa ataatatctt taggtatctt ataagtcttt ctaaacttat
    11101 tgaatatatc ttttttacca gtaaatattt tttgatgtat aatctcttct ctaattgctg
    11161 ataaattcca ctgagcagtg gtaatcgtaa tgtcttcaaa taaaatttca ggaatatata
    11221 ctctttcctg agccaaaata tcccaaggaa ataatgacca atgcgataaa ccgtactcaa
    11281 actcagataa atcaaccaac aatctcaaaa ccctgctttg gctatcggaa tttagcatat
    11341 tattcatggt aaaattcaac tcatttttcc catcgatagt tgtaaaatga aacttaccat
    11401 cactatatct tatataaata tcctccaatc gcagttcgtt ctctgaatcc catgatgtgg
    11461 taaaaaatga agtattataa gactcatcac tataattttg aattacattc gcaagtctta
    11521 tctgtttagg aacaaatgac aactctacat tcgtattatt ttgatggttt aaattcttaa
    11581 ttgtttctct tactgtttcg cttaatccat agaatcttcc aaagcttcta cctgcttgtc
    11641 cagaaccgat attagctcct aaatataact tcacatcatt atttattatt tttatattaa
    11701 aatttaattc aaacgaatct aaactattgc tttgatctgt ttgaaaatca tctaaatctt
    11761 ttgaataaaa ttcgatatgc cctccattta atagagcttc ctcatatttc ttttcaaaca
    11821 tttgcattat tggatcgctt aactctattg cttcaccttc tcctcctttg taatattcag
    11881 gaaaacctat gcctgcatct ttatttatta cttcaacaaa aggaattgag cagttttctc
    11941 catatttgcc aataaatttc aactgatact cttcaagtga atttttcctc ccccctcttc
    12001 tctttacatt tgtatttaat tttacaaaca tctctgcgag tcgttctata ctttcccgag
    12061 ccgtttcaga tagaatatta aaaccgcctt catactctgc taatgaatca acttttaagt
    12121 aatttccacc gttatcatat ttataaatac tttccatttt atcaaaaatc tgcaaaagta
    12181 agtctctacc ttccccaata tttaaatcgt tgtaatcttc aatcatcttc tttaaagtag
    12241 ttaagtcagt acatagtgtt ccttcttcta atttagatag tagataatct aaggaattga
    12301 tattacaaat tggtggtctt aaatctgaaa ttaaaaaatc atttgtaact aagtcgtgta
    12361 tatatgactc tagcatgtct gacactatat ctggataatt atttttaaat acatctatta
    12421 actcttcata ttttatatat cgagttttac atttttctac aagtaactta attggttttg
    12481 aaaaacttat gtttctatct tcctctgatt cattcactgt aaatggtagt acatatctgt
    12541 caccttgagt aaacgccgct gtatttattt ttaatgaaag tttttcaagg ttacttattt
    12601 catacgattt tactagagaa tatatccatg aataatctgg gactgctttt ttaataaaac
    12661 taaccttttg tacattagaa gtggttccct ttgtcaaatc aactgtatta atagcagtaa
    12721 agataccaaa tggagtagtt ctaaacgctg ctcttttaga ataactattt aatgagactt
    12781 ttacattttg ctttttcttt tcattacatt tatcaaaatc tattacacta tcataaagac
    12841 tgggactagc aactaaaagt tgttccataa aaactttatc atttattaaa tcttcatagt
    12901 ctttattata aaattcttgt ttttgcaatg gaatacgaat cataccagtt ggaaatacct
    12961 tatacatttt gttctccttt tcaaattaaa taaatattct ttgccacaat agctcctcgt
    13021 cactgtaagg atttaataag aataacacta tccctgatac cccttgcaat atccctattg
    13081 actccagttc aacttctatt tctccaaatt tttctctgtc agtataccca aatacttttg
    13141 tagttgaaaa cgatgtaatt atttgttggc gaagctcttc tgcaaagcga gttagcctct
    13201 cacttgaata atttttacca atcaagtcat atataaaagt tactcctgcc aaaccatggc
    13261 acattgtaag agaatagaca tttttgtctg aacttctcat ctgagcaatt aaactttcat
    13321 taatatcgtc aataaaattt aaatctatca attgacacat tttaaaaagg gcaactgcag
    13381 ttccaggcgt accgtaacac catgccattc tagtaggaag ataatttaaa tcaagatcca
    13441 aaatattact agtataaatt tttccaggcc atagtttgtc atcccttcta aataaaaaca
    13501 ttaaatttat agctttattc agtatatctc ctacttctac actcaaaatt ttttctgatt
    13561 ttaattttgc taggattagc ataggaccag taattccatg tgcaattcct agatcaaatt
    13621 cagcttctct atcttcagaa caattagtta ccttatttaa aattgtctca atatatttca
    13681 aaagataatt tccaatttga tttaactcta cagaaaaaaa ttcttgttca aggagtaagt
    13741 aattagttat tccactaaat ccgttaacca tatcataatc atatggctca ataattcctt
    13801 tctcaatatt agaaaaactt actaccaatt tgtctttaat ttgttctttt agaagacaat
    13861 ttaaggatga taataatttc ggataatact ttccagatat actcgcttct ttaatagcta
    13921 atgcaatacc tgcagtgcca gcatagagag acggagattc tataccatct ctagagatta
    13981 tgtctactaa tttctcaacg tatatgttgg agaatgttag atatctctca tcttttgtca
    14041 ctttatatag tgaactatac attaagatta atgatggcac accatgactc agagatttat
    14101 caaaaaaggt atttctatat ttagattcaa tattaattag tactttattc tcactttcag
    14161 ctactatctg ttcataattt ataaaactat ctgcaatgta atctactatc tgtttaattt
    14221 gaggtgtcat atgtggctcc aatttcttta tctaataagt ttttaaactt cattccttta
    14281 atatttaaat cagcgaaact tcctgtttca gctatacatc cattatctag atacaaaatt
    14341 ttattcacag attgagtaat gcttggctta tgcgtaataa aaataccaat tttattttgc
    14401 gataattcaa aaaatgtttt aaaaattttt gtttcagact caggatctaa cgcagaacta
    14461 ggctcatcta gcaaatagca atccgcttct ttgagatata ctcttgataa tgcaattttt
    14521 tgccactgtc caccagataa ctgtctccca tcctcaaacc aattccctaa ttgctgttcc
    14581 agatctatct gatttaattt ttcctgatta aagtaaaata tcgaatcaac tcctgtatac
    14641 ttcagtgcac ttattaaatc ctcatcacta tataactttg ataattcacc caatccaaca
    14701 ttttcacgca gtgtcaattc atactttaaa aaatcttgga acaatgcgga tattctcttg
    14761 tagtatgact ttttctgtat tgaacgaaga ttaataccat ttatatatat attgccttca
    14821 taattatcgt aaagaccgca ggctaatttg aaaatcgtac tttttccaga accattttca
    14881 ccaacaattg ctaacctctc tcctgcttta attgaaaaac ttatatcgtt tatgacatta
    14941 tccttaccat tatatgaaaa agaaaccctt tcaaattcaa ctgactgaat tgtatcaacc
    15001 gtactttcga ttatagggtt atgtactaac tcatcatgcg taaaatcata caataactcc
    15061 atgtacaatg aaccattata gaaagaatag atattattta ctatattttg tgtacttgtt
    15121 tgcattgtag ataaagacct tataaaagta gttacatttc caactaataa tattcctaga
    15181 aatgcttctc taactgcaag gaacataata atagattgta tagcaatctc caatacacct
    15241 aatactatgc cgtatttcat tttagatcta tttatactat tttcctgatt aataaataat
    15301 tgattatttt tatcatattt ttttagaaaa taactttcta acgtacctaa cctcaactcc
    15361 ttgacagcaa aatcatgtgt taaaagatga ctaatatacc aatttgctct ctcatcgcta
    15421 ctccttttat atagtatctc aaattcattt ttaccaattt tcaagtagat atatatagaa
    15481 acgataggaa ggataagtaa aagtggaaaa attgatatct tccaatttaa taaaataaca
    15541 aatgaggaaa ttaatgatac taacgaagat aatacagaaa taattgactt atatatttga
    15601 aaaggtttat aagatatttg attttgtatg cgagtgatat catcatagat ttctggtgtc
    15661 tcaaattcag ccaatgttaa ctttacagtt ttctgcataa ctaaatggtt catcttatag
    15721 tgaagaagta tctgtaattt ggtatcaata taactatcaa cttctcctat tataattgtc
    15781 aataatgtta aagcaatata tgagataaaa gcaatcatca atgtgtgaaa attttttata
    15841 tcagtcgtta taatgtttaa cattttttga actagtagca tactaaaaat gggtaaaatc
    15901 ccttgaaata agctaatagc aattgagaac accagtaggg agaaaccagc ctcttttacg
    15961 agatgaattg tttttattag attctttagc tgagctttaa tcatgtataa cttccttatc
    16021 tactaataaa ggagcaaact aaaatcagtc tactccccta aatttataaa tatatcaaat
    16081 tatttgaaat tataatgttg atgataatga taactttatt atttaacagt tgttgtaaag
    16141 caaactaatt tccaacaaga accaggggta cataggtaac ggctaccaac ataaggtgta
    16201 gcagtgtctt ttttatttgt tttcaaatcg agatcaaagt ctttaattgt gttattcatt
    16261 ttctagttcc tctcttacgt ttttgcatcc gatatattta catgattatt atgacacccc
    16321 aagttcagaa tttgttcaga aaaaaagtat tttagccaaa aatcacaaaa atttacgatt
    16381 ttttgcctga aatactccag tttcaatcaa atctattaaa ttttcttttt tatcgagatt
    16441 atggcgctag cacctcctct agctggatta ttcaattgaa gattcccacc gtgtttgata
    16501 gcaacgcctt taacaaatgc caaaccgatt ccataatgtt tgttacctct gctataatct
    16561 tccgtgtaaa ataatttatc tccctttttc agtgtactat cactgaaacg ctcaccatta
    16621 ttccatattt caaaatgaat ttgctctcct gactcagaca gtataacttc gattttttta
    16681 tctaatacgg gcataaatct tattgcatta actaaaaggt tagaaagagc tcttatcaga
    16741 ttttcctcat ctccataaaa agaagtgata ataagtctat tacaaataga aaattcaata
    16801 ttattgatat tcaataagtc atcgacttca aaatgtaact cacttaatag ctcctcaact
    16861 aaaattagtt tcactggtct atcctctgaa agcatgcgtg tataagatat taaagaattg
    16921 aaatagcctt caaaaacaga aatactattg ttcatagata caatataacc ttcctgattt
    16981 ttatttaaag tagtcaattc taataactca agatttcctt tcaaaaccgt taaaggtgtt
    17041 tttatatcat gcgaaagtgc tgcaatttga aaggatagat cttgcttttc caatatttct
    17101 cgctcaatca aacttttcaa attatcacct ttaatatgta aagttctaag tatatcatca
    17161 aactcgatta ttttagaata attttccttt tcagaaagga cttctatccc cattttattt
    17221 gctaacttct tgatttcctt gaaattcaaa gaaatttctt ttacaaaccg tgttaaagca
    17281 actacaatgc tgatactcgt tcctattccc agaatataaa atgtcaacat gttatatgaa
    17341 acatttctaa ggtaatggtt agagaactcc ggtatctcat tatatcttat aacaatatta
    17401 taattgatat tagtatataa atcataatgc acagaattaa aagtcagatt atttttttct
    17461 tctgcaactt cgttaaataa agaaaattct ttagataaat attttccttc catagcttta
    17521 tctgtttttc tatcaaaaac aacataatct aatcccaatg tcttcatata attgttcaac
    17581 tgtttgattg actgtttatc tttaatataa aattcttctc ccacattatg agatgtcact
    17641 atatttaact ttgaaaaaga aagcgatatt atcgcaatca ttaaaacagt gatacaaact
    17701 ccaaagacaa tttcaacgac ggcgcgccaa acacgatttt ttattgaata ttttttaaac
    17761 atcccattta taaccaatcc cgcgaatagt ttttattgga ttaattccaa aacttgaaaa
    17821 tttcattcta atctgataga tatattctga aatagaatga aacaatgctg aagcttcatc
    17881 gctatatact tgttcatata tctcttcttt tgtgaaaact ttatacggat gacgtgacag
    17941 taattctaaa atttcgtact ctctataagt caggaataac ggctgtttat caatatataa
    18001 catacgttcc tctagataga tttctatagg tggaaaactg cgcttcactc tctcctcagc
    18061 ctcattcttt atctttgctc tctcttctct cttcaaatga gagttaactt tagctacgaa
    18121 ttgttttaag ctaaatggtt tcacaataaa atcgtctccc cctatattta aagcttggac
    18181 aatttcatta tcgccatcta aagcgctaac aaaaataatt ggagaatgaa cttcttcccg
    18241 aattttataa caaaactcag ttccacttat attaggcatc atgatatcca ataagatcaa
    18301 atcatatcct ttaaaatcat caaaaactat tggtaattct atctcttgac aggttataac
    18361 atggtaattt tcaatctcca aagcagtctt cataagcttc agaatttctt tatcatcatc
    18421 tatggctaaa atcttataca ttattacccc ttttaaataa tgaaatactt acaaataaac
    18481 tattctcaat gtaagcggca ggaatcattt ctttaatata gttatttacg aaacatcaac
    18541 tagattttaa tatctgatat aaaaattgct accacccaaa ttatatcata attactccca
    18601 ttgtatttca tttcgagaag tatcgataat tttgctataa ctcaagatct ctccaaactt
    18661 ttccaacctt cttactagat attcatactc atctatggat atattaagtt cattttgaga
    18721 agtttcaatc tctttctcaa cttcttctat ttttatagct gcagtcaagt tcatcttggc
    18781 atagtcatat ctggccaaac ggcggttctc tatatcgttg ttgttcagat taagcaagac
    18841 ctccgcaacc ttattgagat gagcaatatg gtcctgcaac tctgtaatgg tcaaatctaa
    18901 ttgagccata tctttaacaa tgtcatcttt aatatcttga taaggcttat tctccgtatc
    18961 taactcaatg aggagactta tttcttcaat ctttttctta agtgtatcaa tggttttggt
    19021 cgtcttcttt ttggaatact cttgctatca ttggttaact ggtaaatgag ctcacgccct
    19081 cgcatatagc gattaagttt agacgcctcc ttgttgtaaa tgaagaattg tgcggtctca
    19141 cgaatataga ctttatattt tttatgattt tcttctttcc taatatccaa ttgatagtta
    19201 ggaatgaaaa taagaccact ctgctttaca ccaaaagaca ccttgatata aatgtcctca
    19261 gcaattaacc tctcaatttg atgttccaca agttcaactt caaactcatg gatggcatct
    19321 cgttgcccct tgtatttctt gaagacttcc ttactctctt cagtggatac ctcctcttta
    19381 tctcgttctt cttgaaaagc atgtactgtt tctgtagact ttctaatcga tctgaaacac
    19441 cgacttcctt atttttaaag taatcttgaa aaaattgaca tcatataatt tcttatcgct
    19501 tattttttta tgacttaaac ttatcttttg agtatcttct tctaggataa aagttacatt
    19561 ttttgtttta agtcaatggt tagattcact tcttttgctt ttaatagtag ctcctctaat
    19621 gaattggcac ggttcaacaa aaattctaat cgactttcaa tttctatctt ggcaaaatgt
    19681 gtacgaaaaa attcttcatc atataaatct cgcttactga gttgacggcc tcgaattggt
    19741 tttttcatta ctcgatccgt catcatgaat ctgctatgct tctgactaaa atcaatatga
    19801 acatgtaatt actctgcttt ttctaaaaaa tcatcaaaga actttgactg ctgcatcaaa
    19861 aaataaagac gttgtttcaa ttcaaattta tgactagact gcctatattt ttgatagtca
    19921 cggtaggaat aacgcttctc gataattttt gctccagtca tttttgaaat acggtctgaa
    19981 atcatacgga ggtttcgttc caaggcataa ttccatatca attttttatc tgaattacgg
    20041 tcaatggcgt tgattggatg tgattatggg tatgattttt atctgtttgg tcgctcgagt
    20101 aatctcaaag aagagagttc tcctagtgcc ctcaaatccc agtcgtctgc ctgcgagtga
    20161 aaatgcttga caagggaaaa accaccgcag atgatatcca cttgccctct aagttgtcta
    20221 aagtcttggt ctgtgacttg tctaatgtca tgaaattcta tttctccttc ggtctcatag
    20281 atggctttgt aagattgcct cgcaaactta tcaatctcac aaaagccaat acactcatgg
    20341 cactgattaa ttaaacccaa tcgaaagcct ccaattcctg caaacaaatc taaaaactgc
    20401 atatctctca ttcctttctg acaaaataaa aggaccttac tgcttatgta acgtccttac
    20461 tcaactataa tcttccattt ctggtgcagt gataacttca ataatcaacc aaattgtcct
    20521 tagtccccaa acacctaata atagccacca aaacatctta ctcacttcgc ttcttgtcac
    20581 cttagttgct tggtccattc ggataagaca ccattaaaga catattccgc aatagcttca
    20641 gctgttcttg cgaaccgtat gtgttccagc gcataagcat agcgttcttc aaaatagctc
    20701 atgactaagt cactagcagc ttgtgataaa ccaccttcac gtaattggga atgaaccaaa
    20761 ctccaaatat aatctttgtc atacttggtg acatgatcaa cttgacgagt aaagtgaccc
    20821 tcagtctttt tcacactagt gcactccttt ctttcctcct cgtcctcaat gactaaatta
    20881 ctctctttag tttcactacc ttcagtttca ctcttttcag tctcactagg ggctgaattt
    20941 aacacggggc tggtctgttt ttgagactgg ccccgtctga acttaagact ggggtgaaat
    21001 catgttccaa ctttcctaaa taaatcttat tagccaaacg ccctttttca ctgggagact
    21061 gctggccttc atcgatcaaa ccatagtctc gtaggttttc ttaatcacta acaatttcga
    21121 cttggaacag ccaaggagta ctatcatttt gagttggaat aaatgagata aatggcaatc
    21181 accatttgtc gtgatgactg ttcattcatt tgcatgattt tcctcctgaa aataaaaaaa
    21241 ttattactag acacatatag atagaataac cactctactc agcttaattt ttatagaaaa
    21301 taaaaatcct gccaaaaaaa tctttggcag gatttttggc aaaaaaccaa atcattttat
    21361 caatttcttc aaatcgctat aagctctaaa agactgataa atcgggattt cgctctcaaa
    21421 ttgctatctg cttcttattt aagagtgatt gttgctccag cttcttcaag tttagctttg
    21481 atttcttcag cttctgcagc agcaacgcct tctttaacgt tagcaggtgc tccatcaaca
    21541 agacctttag cttctttaag accaagacct gtgatttcac gaacagcttt gataacgcca
    21601 acttttttgt cgccagcaga tgtcaattca acgtcgaatg aatctttagc agcttcttca
    21661 gcaccaccag cagctgcagc agctacagga gcagctgcag ttacaccaaa ttcttcttcg
    21721 atagctttta caagatcgtt aagctcaagg attgaagctt ctttaatttc agcaataatg
    21781 ttttcaatgt tcaatgccat tgtgatttcc tccaaataat taatttaaaa tgttaagcgt
    21841 agccttaagc tactctgaga cgtcttaagc agcgccttct ttgttttctg caacagcttt
    21901 gacagcgtat gcaacgttgc ggactggagc ttgaagtaca gaaagaagca tagaaagcat
    21961 tccttcgcgg tttggcaatg ttgcaagtgc ttggatttct tcttttgaag aaacagcgcc
    22021 ttcgattgca ccacctttga tttcaagtgc gtcagcagtt ttagtaaagt cgttgataac
    22081 tttagctggt gcgataacat cttcgttaga aaatgctact gcagatggtc ctacgaatac
    22141 atctttcaac tcatcaagac ctgctttttc agctgcacga gtcaaaattg agtttttgat
    22201 aactttgaac tcaacgccac tttcacgaag tgaacgacga agaacagtat cttgatcaac
    22261 tgtaagacca cgtgaatcaa caacaacgat acttgctgct gctttcattt tctcagcaat
    22321 aagttcgact tgttcagctt ttttagcaat aattgcttca ctcattagtt ttacctccgt
    22381 ttttattttt gggctaggta caaaaaaatc gcacctaaac ctagacacga aagtacaaat
    22441 acgttatttt ctttaatcac gttttgtgcc tcggcaggaa tattatgagc atgctcccct
    22501 gctgtcttag gtcagttttt tatcaaaacc tatattatta tattctttta taatactatt
    22561 gtcaataaaa attattaaaa aaatgatttt tttacattta ttttttttag tattagctaa
    22621 actcaaaatg gtgctagtcc tattttctat acaaaataat accttgcttt ctactaggac
    22681 ttgatttaaa atagagggca tgagtataac taaagaattt gatacaatta ccgccatttc
    22741 aacccctctt ggtgaaggag ctattggcat tgttcgtcta tccgggacag atgcgcttgc
    22801 cattgcacaa tctgttttta agggaaagaa cttagagcaa gtcgcctcac acaccattaa
    22861 ctatggccat atcattgatc ctaagacagg aactattatc gatgaagtta tggtgtcagt
    22921 catgctagcc cccaaaacct ttacccgtga aaatgtggtt gaaatcaata cgcacggtgg
    22981 tattgctgtg acaaatgaaa tcttgcaact attgattcgc caaggcgcta gaatggcaga
    23041 gcctggcgaa ttcaccaaac gtgccttctt aaatgggcga gttgatttga ctcaagctga
    23101 ggctgtcatg gacattattc gtgccaaaac agacaaggct atgacgattg cagtcaagca
    23161 actggatggc tccctttccc agcttatcaa tgacacccgt caggaaattc tcaacacctt
    23221 agctcaagtc gaagtcaata ttgactaccc tgaatacgac gatgtggaag aaatgaccac
    23281 tgctctgcta cgtgagaaaa cacaagaatt ccaaagcctt ttggaaaatc tccttcgaac
    23341 agctaaacgc ggtaaaattc tgcgtgaggg cttatcaaca gctattattg gtcgtccaaa
    23401 cgtcggtaag tcaagcttac ttaataattt gttgcgagaa gacaaggcta ttgtgactga
    23461 tattgcagga accacccgag atgtcattga agaatacgtt aatatcaagg gagttcctct
    23521 aaaacttgtc gatacggccg gtattcgtga gacagatgat ctggtcgaac aaatcggagt
    23581 tgaacggtct aaaaaagctc ttcaagaagc tgaccttgtt cttcttgtgc tgaatgcttc
    23641 tgaaaaactg accgaccaag accgggcttt gttaaatctc agtcaggaca gtaaccgtat
    23701 tatattgttg aataaaacgg atttggaaca aaagattgaa ttggaacagt tgccagcgga
    23761 ccttattcct atttcggtgt taaccaacca gaacattaat ctgatcgaag atcgtatcaa
    23821 ccagctcttc tttgataatg ctggtttggt agagcaagat gccacttacc tgtcaaatgc
    23881 ccgccacatt tctttgattg aaaaggcagt ccagagttta gaagctgtca atgacggctt
    23941 ggcgcttggc atgccagttg atttactaca ggttgattta accagaactt gggaaattct
    24001 cggagaaatc acaggggatg ctgctcctga tgaactcatt acccaactct ttagtcaatt
    24061 ctgtcttggt aaataacaac agtctctaat catcacaaaa gagctggaac agtgttccag
    24121 ctctttttgc tatcataagc atcttattta attaattcgt agattgcttc agcataaata
    24181 gctgctgagc gataaatatt ttctaaaggc atatattcat tagcttggtg
//
