LOCUS       NZ_CP035435            11893 bp    DNA     circular CON 13-OCT-2024
DEFINITION  Streptococcus pyogenes strain emm64.3 chromosome, complete genome.
ACCESSION   NZ_CP035435
VERSION     NZ_CP035435.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1779766)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1779766)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005161325.1-RS_2024_10_12
            Annotation Date                   :: 10/12/2024 01:25:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,698
            Genes (coding)                    :: 1,645
            CDSs (with protein)               :: 1,645
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 26 of 53
            Pseudo Genes (incomplete)         :: 27 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 13 of 53
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:28:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1375897
            Orig. end                         :: 1387790
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035435.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11893
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11893
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11893
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6893](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6893)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            208..450
                     /locus_tag="ETT64_RS09405"
                     /old_locus_tag="ETT64_07115"
     CDS             208..450
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="ETT64_RS09405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07115"
                     /product="hypothetical protein"
                     /protein_id="WP_011285423.1"
                     /transl_table=11
                     /translation="MKQQGHTVMMCLDKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLH
                     LLPKVVPVLIHLVDKANVSWLTVILQLPILLKTIW"
     gene            972..1241
                     /gene="stcA"
                     /locus_tag="ETT64_RS07125"
                     /old_locus_tag="ETT64_07120"
     CDS             972..1241
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="ETT64_RS07125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07120"
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_011054255.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPILKHTIERKRTAMTIGHSK
                     QNLHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(1561..1959)
                     /locus_tag="ETT64_RS07130"
                     /old_locus_tag="ETT64_07125"
     CDS             complement(1561..1959)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /locus_tag="ETT64_RS07130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07125"
                     /product="hypothetical protein"
                     /protein_id="WP_009880375.1"
                     /transl_table=11
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLED
                     DAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTGIT
                     YGDDTLADLTHHLLHSDGVLPSLNDKSA"
     gene            complement(2052..3122)
                     /locus_tag="ETT64_RS07135"
                     /old_locus_tag="ETT64_07130"
     CDS             complement(2052..3122)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="ETT64_RS07135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07130"
                     /product="hypothetical protein"
                     /protein_id="WP_136292972.1"
                     /transl_table=11
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSFHSSDRRFGVG
                     YKEETTSQFFSDQPSQVVLYRGEANTVRFTYTDQMYLMKDIRIALDGSGKSLTAQTVPG
                     MGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGALPDNIKVSIN
                     SKGGQATITYGDGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQSLNEH
                     EDMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVGSWLEPA
                     TALPSVEMSAEDRLKS"
     gene            complement(3373..3774)
                     /locus_tag="ETT64_RS07140"
                     /old_locus_tag="ETT64_07135"
     CDS             complement(3373..3774)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /locus_tag="ETT64_RS07140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07135"
                     /product="hypothetical protein"
                     /protein_id="WP_136292973.1"
                     /transl_table=11
                     /translation="MRNILRFLGIIIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLTWYQVVKQFWLFIKCIILIPTLIAFIIIIGCLTSISEVSIYFYLHL
                     IVVCYTIGMILSLGRLISPNHSIFNKLKK"
     gene            complement(4300..4494)
                     /locus_tag="ETT64_RS07150"
                     /old_locus_tag="ETT64_07145"
     CDS             complement(4300..4494)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /locus_tag="ETT64_RS07150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07145"
                     /product="bacteriocin"
                     /protein_id="WP_136093874.1"
                     /transl_table=11
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMAGAAQGVTVCAQTGVFIPW
                     QGYILCGAAGAATNIIRPH"
     gene            complement(4788..4988)
                     /locus_tag="ETT64_RS07160"
                     /old_locus_tag="ETT64_07155"
     CDS             complement(4788..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="ETT64_RS07160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07155"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_003060803.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LTGGCVVGFTVGGAYLGYTAN"
     gene            complement(5001..5228)
                     /locus_tag="ETT64_RS07165"
                     /old_locus_tag="ETT64_07160"
     CDS             complement(5001..5228)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="ETT64_RS07165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07160"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_111702361.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.6e-17,
                     bitscore: 59.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLSKVEGGKNNWQANVSGVIAAGSAGAAIG
                     FPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            5546..6231
                     /locus_tag="ETT64_RS07170"
                     /old_locus_tag="ETT64_07165"
                     /pseudo=""
     CDS             5546..6231
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /locus_tag="ETT64_RS07170"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07165"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTQFTTELLNFLAQKQDIDEFFRSSLEIAMNDLLQVELSAFLGYE
                     PYEKKVATQVIAVMGLILVSLKQSMA"
     gene            complement(6451..6633)
                     /locus_tag="ETT64_RS07175"
                     /old_locus_tag="ETT64_07170"
     CDS             complement(6451..6633)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /locus_tag="ETT64_RS07175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07170"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_002987564.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.8e-07,
                     bitscore: 27.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITGG
                     AAAFVCVASGIAAYQ"
     gene            complement(6648..6893)
                     /locus_tag="ETT64_RS07180"
                     /old_locus_tag="ETT64_07175"
     CDS             complement(6648..6893)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /locus_tag="ETT64_RS07180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07175"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_002994504.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.9e-15,
                     bitscore: 53.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGGVGAG
                     MATLGVGGVAGAFAGAHVGAIAGGLTCVGGMLFNGK"
     misc_feature    complement(6850..6864)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.12"
                     /tool="antismash"
     gene            7290..7544
                     /locus_tag="ETT64_RS09330"
                     /old_locus_tag="ETT64_07180"
     CDS             7290..7544
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="ETT64_RS09330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07180"
                     /product="hypothetical protein"
                     /protein_id="WP_011184258.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSEQVYDLLNEIRMRYPHAHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHKSLKSIRDQIQLYLDELYNSNNG"
     gene            complement(7850..8325)
                     /locus_tag="ETT64_RS07190"
                     /old_locus_tag="ETT64_07185"
                     /pseudo=""
     CDS             complement(7850..8325)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="ETT64_RS07190"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07185"
                     /product="NUDIX hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPQDYISYIRSKVGMIRLFLTLLVGF"
     gene            complement(8345..9241)
                     /gene="era"
                     /locus_tag="ETT64_RS07195"
                     /old_locus_tag="ETT64_07190"
     CDS             complement(8345..9241)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="ETT64_RS07195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07190"
                     /product="GTPase Era"
                     /protein_id="WP_002985743.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     misc_feature    9294..9309
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            complement(9361..9768)
                     /locus_tag="ETT64_RS07200"
                     /old_locus_tag="ETT64_07195"
     CDS             complement(9361..9768)
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="ETT64_RS07200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07195"
                     /product="diacylglycerol kinase family protein"
                     /protein_id="WP_002985746.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            complement(9749..10246)
                     /gene="ybeY"
                     /locus_tag="ETT64_RS07205"
                     /old_locus_tag="ETT64_07200"
     CDS             complement(9749..10246)
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="ETT64_RS07205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07200"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_002985748.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     misc_feature    complement(10260..10274)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            complement(10405..10980)
                     /locus_tag="ETT64_RS07210"
                     /old_locus_tag="ETT64_07205"
     CDS             complement(10405..10980)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="ETT64_RS07210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_07205"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_009880369.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
ORIGIN
        1 taataaagct ttgcttttta cattgatcat ggatatcctg attatcgttg gtggataacc
       61 tttcgcttaa aaacaatcca cttttgggaa acccttatat ttagtttgta acaggaaaac
      121 taataaatat taaaaagagt gttagcgacc tagcggcagc tttactctta agtgcgtttg
      181 ctgccattga cagcaaggtc aaggctgatg aaacagcagg ggcataccgt tatgatgtgt
      241 ctggacaaaa cacttactat caaactatca gctccattaa ccagcaacga cctggtgcca
      301 ctctcgacga atgaggtgat tattatggaa cccttacttc atttacttcc caaagtcgta
      361 ccagtactta tacacttggt agacaaagcc aacgtatcat ggttaacagt gatattacag
      421 ttacctatac tcttaaagac aatttggtag cccttcataa ggaatataaa acctttgtca
      481 aaaaaatatt acagataaac cgatcaccag tgactacgac aaggctaagg ttatttataa
      541 ccatttaatc aacgaataca cttacgccac aaaataactt agcaccaatt gcaaaaccgt
      601 aagcagcatc aacaatcata ctcctaaagc tctttatcgt ggcaagtgtg gtgtttgtca
      661 agcttatgcc gtcatgttta aagatatggc agaagctgca ggcttatcag cttggtatgt
      721 gataaaaaag gattgaatga gcaccatgct tgaaacatcg ttaccattca tggtgttaat
      781 tcctacgttg atgctacttg gaatgattca gcatacacca caaggtattt ccttgctgga
      841 aaagacgtga ggaatactga gtaccgttta gatagtcagt acaaccactt ggctaatgct
      901 attttaaata ccagctacca aaaaggctaa actaacaaca actacatttt tcaactagaa
      961 aggcctaaac aatgaccaaa aaacagtttt atcttatctt agctattagc atactgtgtt
     1021 ttattcttgc cgtcagccct aaaccaccaa ttcttaaaca cactattgaa aggaaaagga
     1081 ctgcgatgac cataggccac tcaaaacaaa accttcacaa aaaagaagcc cttaaacaca
     1141 aaaaaaacaa aaataaggct ctttttcttc caagtgggga tagcgctatc aaatctgtta
     1201 ttagcatgac agttagactc ctgttagatg accaatctta gctctcctaa aatcagtgga
     1261 cactaccgtt tcggtagtgt tcttttttaa gctaaaaaac tgatcatgaa agagcaaccc
     1321 catcacccta acagcccact ttacccgcac tttcttaaaa agttaggaga aaacgatata
     1381 ccaattattt tctgatccca gctccctatc attaacaaga tgtgtagcac ggacctacat
     1441 acaggtaagc tagagtcatt ctcgctattt cggacaaaaa gaggccggta gttccctgac
     1501 ctcttcttta tttatcttgt aacactcctc ttcttagcga gacttactag cacataattc
     1561 ttaagcactc ttatcattta gactaggaag aacaccatct gaatgaagca aatgatgcgt
     1621 cagatctgcc agagtatcat ctccataagt aatccctgtt tggtgctgtt tctgatgaaa
     1681 atcagatgac tccttaactt tagcagtcac ctgctggtca cttggatgaa aattaatttc
     1741 tctcttggct tcagccgcaa ctggctcgag gaacaactta ttgacattac ggtcttcctc
     1801 caaatgatta aggatagcag catcatcttc taacgaaaga accttcttgg gtaacaaaaa
     1861 ggggttattt agccttggct cagtaacgac ttccttggct actcgtgata gtttattatt
     1921 gtcttcctca ggtatcaaac ggttaggcaa tcgatgcaat tcttccggct gtgaggcgct
     1981 cacagctact tcagaagaaa cctccaagcc ctttcctgtt tgcctgtcta aagcaaccgg
     2041 caaattatta actaactttt taacctgtcc tcagcgctca tttcaacact aggaagagct
     2101 gtcgctggtt ctagccagct accaacttct gctggtctta ccttaacatc gctggcaggc
     2161 ttctccttct catcttcatt aggtctaaaa ggctcaaacc gccccgttga tttttcatct
     2221 atgaccttta tattgtcttt tactacatga gacagtggtt cgacatttac catatcttca
     2281 tgctcgttaa ggctctggtc tcgtttgaat ttaccaggaa taccaggagt cggtgttccg
     2341 acagattgat cggcttcagt aggctctttt acggagcctt ttttccataa aactgctgga
     2401 ggaataatat ccgtagagcc gtccccataa gtaatcgtag cctgcccccc ctttgaatta
     2461 atagatactt taatattgtc cggtaaggct ccactacctc ctgtggttaa aagggcaccc
     2521 gcatttttaa tgagagttgt cattcgttca ataccagcaa cgccaatatg gttcaagtca
     2581 tctactggta actgatcaac agatttcata gtgtatttcc aagcttgcgg ttgaacaaga
     2641 aaaacagttc ctttatacat tgtgttatgc atatcatcaa tgactttgaa ataccttgta
     2701 tatttggttt gtacattagg gttagcaacg ggaacagtgc tttcaggaac tcctgacatc
     2761 gtaaaaatcc ctctagcaga agtttgaaag ccctcataaa catgacccat accaggaaca
     2821 gtctgagcgg tcaaagactt accagaacca tccaaagcaa tgcgaatatc tttcatcaga
     2881 tacatctgat cggtataggt aaacctaacc gtattagcct cacctcgata caaaaccact
     2941 tgggagggct gatccgaaaa aaactgagag gttgtttctt ccttatagcc aactccaaaa
     3001 cgtctatcag acgaatggaa tgaatctgcc gcaactgtac tactcaaact gactagtaaa
     3061 gtagccgcac aaccaactaa taaactgctc gttttaactt taacatctct taacatcatc
     3121 atgttccttt cattattttt tatttaggaa ttcgtcatac tatattttct catattagtc
     3181 taacaaaaaa ctcgtaattc tatataaatc atataaaatg acgagctttg gcaaagatga
     3241 gtatagcagg atggaaaaca gattgtttct gaaacttcaa aggtatcttc ttgtcacgaa
     3301 ctattcttta attgaatcgg ttacactaaa ctagtcccat tatttaaaaa caaactttaa
     3361 atattattca tttcactttt taagtttatt aaatatagag tgattaggag aaataagacg
     3421 acctaatgat aaaatcattc ctattgtata acaaacaaca ataaggtgta aataaaaata
     3481 aattgatacc tctgatatac tcgtcaaaca tcctattatt ataataaaag caattaaggt
     3541 aggaataaga ataatacact taataaataa ccaaaattgc ttaacaactt gataccatgt
     3601 gagcacttct gtcgtttgtc tttgtttgta gacaaaaacg aataaatcaa ggataaaact
     3661 ccaacagata agttgtatat ttttcattaa tggtaaatcg tagaaactac ctatactaaa
     3721 tagaatcact gcagaaataa taataatacc taaaaatctt aatatattac gcatatcttc
     3781 acctcctcta ctctatcggt agcaaatata accgaacaaa ttgttattcc tgttatcaga
     3841 gacagttata acttttttaa ccaattttca atacgcattg cgcttttaac tctttagtct
     3901 ctaaagttaa atatcttaag tactgatatt gtataagtca tccattttcc ccttaaatgt
     3961 gattgcatta tagctcagta gaattaatct taatttctgg tatttttttt ccataccaag
     4021 tatctataac gcaaatatag ataaacaata agtataaaat aaataaagat cacaataagg
     4081 gatatacctc ctagtacagc agcccaaata ggataagggt gcctaaagtt gtagactaca
     4141 ctcaagatga taagggtaaa agggacaatg taacctccca gcttttcaac attttctaga
     4201 aatatcatct ttctatcaga tagattaaaa ataatatcta aagttttcat accactcctc
     4261 tactacttat acatcagtga tagatagtca tttatatatt tagtgaggcc ggataatatt
     4321 tgtagcagca ccagctgctc cacacaaaat ataaccttgc caagggataa atacgcctgt
     4381 ctgagcacaa actgtcactc cctgcgcagc acctgccatc cctgtcaaaa agatatttgc
     4441 tccgtctttt ccgcctccat ttatgatagc cagaccattt tttagcactt ccatattttt
     4501 gaagtctttt tcattttaat ttcactcttt tgtattttaa aaagctaaga gtgatatcta
     4561 aattcattgg cataatcact attttctttt atcgataaaa acaaataatg acgctaccaa
     4621 agctacgata gataacatgt ttaccgattt ttccgaaagg gataattgat tgaaaattaa
     4681 aaacactaaa tatggtatag atttatcaac atttttttat gaatggcatc aacaatcatt
     4741 aaaaacatta tagacgcatg cataataata aaaatcgtat agatcattta atttgctgta
     4801 tatcccaaat atgctccacc aactgtaaat cctacaacac atccacctgt caatactgga
     4861 tgtggcagtt tacaaaattt caatccagaa gctaagcctc caagaccacc aattacagca
     4921 tctcctggag cgtttcctcc acttatacta gccatgtcat taattgttaa ttccttcatt
     4981 gttgtcatcc tttctttcct ctagaagccg cctgtagctc ctgttacacc agcccaaaga
     5041 gtaatagctg ttttagctcc aatataacca caggctacac cgcaaactgg aaaacctata
     5101 gcagctccag cactacctgc agcgataacc ccactgacat ttgcctgcca attattctta
     5161 ccaccctcaa ccttggaaag cttttctata gtcatttggt gaaatgtttc cagtttcttt
     5221 atctccatat cgaataactt cttttcataa ttcttaaatt tgtctcgttg caacctcgag
     5281 ctagtacaga atatcataaa gaacagtgtc atggtttcaa aatcttaaaa ggctgttttt
     5341 catcatgaaa tgtcttttta tcctctctac acttacgctg tgacaatagt taattaatta
     5401 agaagtttaa ataataccat tcatgctttt ataaatacct tttaagattt ttgatggtag
     5461 tgtaaaatat cttgtgtaaa cacacaaaaa ggaataaatc ctgtatagta gagttgcgaa
     5521 acttactaga aagagattta ttcccatgac tcagtttacc acagaattac ttaacttcct
     5581 agcacaaaaa caagatattg atgaattttt tcgctcctct ttagaaatag ctatgaatga
     5641 cctcttgcaa gttgaactat cagcttttct tggttatgaa ccttatgaaa agaaggttgc
     5701 aacacaggta atagccgtaa tgggacttat tctcgtcagt ttgaaacaaa gtatggctta
     5761 gtcaatttaa tcatctcaag agatcgaaac ggcgaatttt caccaatttt attaccatct
     5821 tatgctagac gagaggacca cttggaagag atagtgatca aactctatca gactggcgtt
     5881 acgacacgcg aaatcagtga cattattaag cgcatgtatg gtcaccactt gtttctgagt
     5941 ggaagcctcg ttacaaaaag gttatgacat cacttgaaac aacggaaaat ctcctaactt
     6001 tctatcaatt tccacattac atttggtcta gtatttactc tacaaacttg attgaatcac
     6061 tcaataaaga aatcaagcga caaagcaaga aggagggtgg tttttccaaa tgaagaagcc
     6121 ttagaacgat gtcttgtaag tattttcgaa gactataata ttaagttcgg agctcgtatt
     6181 cataaaggat tcggagtatg ttttgacaca cttgatagct tatttgacta atattcaaaa
     6241 actctactct tgtgtttaca caacattatt gacagtctca attaatctat atccctttaa
     6301 tattattaat ttgtaatagt acttagtaag acataaacaa atatcacaat taaaattact
     6361 ttacttgcat tttctcttac ctctttatcc ttcaaaaaaa tttgggaaag tattagacaa
     6421 attaaaccga gtattataac tataatcata tcattgataa gctgcaattc cagatgctac
     6481 acatacaaat gctgccgcac ctccagttat tggacctcct gctgcagcta taagcatccc
     6541 acctaagcac cctccgatag cagatccaat atttcctttt ccaccagaaa tatttactaa
     6601 ttcttcaaac tgtaattcta aaaaattatc catttttcac ctcaaaatta ttttccatta
     6661 aataacatac caccaacgca agttaatcca cccgcaatag cacctacatg tgcacctgca
     6721 aacgcacctg caactcctcc aacacctaaa gtagccatac ctgctccaac accacctgca
     6781 atagcacctg aagtaatacc accaattaaa tgatttttac aatctgtagc actataacct
     6841 ccaataatat ggaacaactc ttctttttgt atttcttctg caaattttat cataacttta
     6901 aaaacctttc taaaatgtta tattcatctt gttgcaactt tgacaaacta aatatagcat
     6961 attattataa aaattttttc tatgtcgtga atggttgtat aaacatctca aataaacttt
     7021 ttaacatatt tgagactgtc aataatgttg tgtaaacaca agagtagagt ttttgaatat
     7081 tagtcaaata agctatcaag tgtgtcaaaa catactccga atcctttatg aatacgagct
     7141 ccgaacttaa tattatagtc ttcgaaaata cttacaagac atcgttctct tttaggatat
     7201 atgatatcta tctatattaa ctattacctc aagagatact ttctaaaaac tcaaattatt
     7261 agtgggaaaa acttagatta taaatacaaa tgtcaggttt attttatcat aaatcatact
     7321 tttttaaatc tgagcaagta tatgaccttc taaacgaaat acgtatgcgt tatcctcatg
     7381 ctcatattat tctaatcgga aatcatatta attacgagga aatatttaaa aatcattatc
     7441 gagtttttgg tgttattgac acaacaagtc ataaatcatt aaagtccatt cgagatcaaa
     7501 ttcaacttta cttagacgaa ctttacaatt cgaataatgg ataggcactt catcaattct
     7561 tttaggaaat gattcttgtc accccttcca agtctattgc cacagttagg ttcaacttgc
     7621 ctaagtcatg catggctagg tggcaattcg tcataactct aaaacaaatg tttgatgtct
     7681 gctagtatca attggaaaga aaatcctgtt aacatttaac aaaattattc agacttaatc
     7741 agaaaaattg gaagcaaaaa aagagagcat aagctctcga aacatcgtct ttcaaaaatt
     7801 tttttaacaa gccaatctcc aaaaatactc caactgtcct agagagttct taatgcccca
     7861 tagcaaagct atcactaaaa tattcctcta acatcaggcg atgtttggct gagacactct
     7921 ctaactctgc tatctcctca tgagagaaaa actgtaactt gagcgtttcc tcattatgaa
     7981 agttatcaat agccatatca gaaacagccg ttagttcata gataaagaca atagtctgaa
     8041 cagcatcacc gttgggataa acttcctcaa aatgagtgta aacattcagt aagcgaacag
     8101 cttcaacctt aattccggtt tcctctaaga actcacgttt gcaagtctct aaagagctct
     8161 cacccagttc catagtgcca ccaggaatag cccaggtttt cttatcacca cgcaactgca
     8221 tcagcacctt gccgtcatca ttggtcaaaa tcccaccagc aaagttaaga ataatcttat
     8281 catgccaact ttggagcgga tataagaaat gtagtcttgt ggcatatgat ctcgctttac
     8341 tatgttaata ttctttctca ttatagccaa aatcagctaa atcaagcttt ttatcccgcc
     8401 agtttttctt gactttgacc cacgtttcta agtagacctt gtcacctaac atgagttcaa
     8461 tatcacggcg cgccattttt ccgatttttt tgagcatggc tccttgtttt ccaatgatga
     8521 tgcctttttg gctatcgcgc tccaccataa tggttgcacg gatatgcact ttatcagtct
     8581 cttcatctcg cttcatggat tcgacaacaa cagctacgga atgtggaacc tcttgttgcg
     8641 tcaaatgcag taccttctca cgaaccattt ctgacacaag gaaacgttct ggatgatcag
     8701 taatctgatc ttctggaaaa tattggaaac cttcttcaag attatcagtt aataacttga
     8761 tcaaagttgg cacattatta ccttctaggg cagaaattgg gaccacttct ttaaaatcca
     8821 tttggctacg gaagtcatca atctgttcca aaagttggtc tggatgaacc ttatcaattt
     8881 tgttgatgac taaaataaca ggaatcttag ctgcttttag ccgctcgata atcatgtcgt
     8941 cacccttacc acgcttttca tcggcaggaa ccatgaaaag aaccgtctca acttcacgta
     9001 aagtactata agcagattca accataaaat caccaagggc ggtctttggt ttatgaatac
     9061 ctggcgtatc aataaagaca atttgttctg tttctgtggt gtaaatgccc ataattttat
     9121 tacgggtggt ctgagccttg tcactcatga tggcaatttt ttgccccatg acgtgattta
     9181 aaaaggtgga tttgccaaca tttggacgac caagaatggc tacaaagcct gatttaaaca
     9241 tagataatta cgggccttaa cggtagggct tcagatcaag cttgtcacta agcttaaccg
     9301 tcttgtcctt tccttattct cctatgccat catcacatag gatagcccat tcctttcttt
     9361 ttaatgaacg aaaatattcc aaattttagg aataaaaata ataagtcctg tcaagacagc
     9421 atagccagaa atcatcaaaa cagcacctgc tgccatatcc ttggcatttt tagctagcat
     9481 agaaaagtga tagtcactag ccaaatcaac tacattttca atagcagaat tgacaatttc
     9541 taaggtaatg accaaaaaaa tggctagaag taaaaataac cattcgatgg ctgaaatgct
     9601 aaaaaataaa ccagcaacac aagctaaaca ggcagacaaa aggtgacttc tgagattgcg
     9661 ttcttcctta aaagctgtga aaacccctgt caaggcaaac tccaagcttg aggtaatggt
     9721 tctgtttttc catttccgtt tggtggtatt attgtcgtgt aaggccataa gcagtcaaaa
     9781 tctcttcctg taaggtaaac atctcttttt cttcttcaag ggtgtagtga tcatacccat
     9841 taatatgtaa gaatccatga acagctaaaa atcccatttc acgctcaaaa gagtgcccat
     9901 attcttggga ctgctcacgc gccttgtcaa tagaaataaa caattcgcca atataagcat
     9961 caaactctgc catcatctct gccaaactag gatcagcagc caaatcctct tgactaaata
    10021 aaataggcgt ttctggctta tattctagag aaatcacatc tgttggacga tcagtatctc
    10081 ggtattctaa gttgagttca tgacttcttt cattggtcac aaaggtcaca gacatttctt
    10141 tttcttcttt gcccgtcttt tgagcagcaa aattgagcaa atcaagtgtt tgctccataa
    10201 tctcttgcga aacttgtccc gtttcgtcaa tcatctcgat atacataagg ttaattgtta
    10261 gggaagggct gcccacctca tttggtatgt tgggcaccca ttattatctg acagataatt
    10321 tcccttatcc tttcaatcag catttgttat ttacccatta taccacaagc cccttaaaaa
    10381 tgggaatgct aggtaaatca gggcctattt ggttagaatt gcctccacac gtttttgcaa
    10441 gataggtaat aaatcctgct caaaccaggg gttcttagct aaccacaatt ggtttcttgg
    10501 ggaaggatga acaagtggaa aatagtctgg caagtaatcc tcaaagtgac ggactgtttc
    10561 agttaaggtt ttgtaagcct tgttaccaag gtaaaagtcc tgagcatagc gaccaaccaa
    10621 aatcgtcaat tcaacttctg gcatcaatgc tcttaatggc gggtgccact tagccgcaaa
    10681 accttctcta ggtggtaagt caccggattt tcccttacca ggataataga aatccatagg
    10741 gataatgcca aacaatcctg agtggtaaaa ggtgtcattg tccacaccta gccagtctcg
    10801 taagcggatg ccactacgat cattccagta aagtttggtt tcttgagcta ctattccagg
    10861 agcttggcca acaataataa tcctagctgt ttgaggagca tcatataaag gaaaaatacc
    10921 tctttcggta taggagagat tagcctcatc tgccataata gctttggtta aatcatccat
    10981 agaatcctcc ttaaaaaggc catattaaac atcatcgtaa aggtcttagt gctcttgacc
    11041 gatcacaggg tattttgtca agcctggctg gtaatcagct tcaacgtcac tagccttggg
    11101 ttttagtaag tccttcgtct cctctgagga cgtttcataa gccttgataa tatcagccac
    11161 aacaggatgt cggacaacat ctttagcaga gaaatagaca aaatcaatct gtttaatgcc
    11221 ttgtaatttc tgagtagcat caatcaaacc agacttgaca tttcgaggga ggtcaatctg
    11281 actcgtatct ccattgacaa tcattttaga attaaaacca agacgggtca aaaacatctt
    11341 catttgcatg atggtcgtgt tttgagcctc atctaagata acaaaagcgt catccagagt
    11401 acgacctcgc atataagcta acggagcaat ctcaatcaca tctcgttcca tcaggcgtgt
    11461 ggtttgttcc ttgcctaaaa tgtgataaag ggcatcgtaa acaggtctga ggtaaggatc
    11521 caccttttct ttcaaatcgc ctggtaaaaa acctaggctt tcgccagctt ctaccgcagg
    11581 acgcgtcaaa ataatacgct ttacctgtcc tcttttcaga gcagtgactg caagcgtaac
    11641 agctaagaaa gttttccctg tccctgcagg tcctacacca aaaaccacat catgacgttt
    11701 gacactatca acataggttt tttgacctaa tgttttgact cgtattgcct taccatagtt
    11761 atctttaatg atttcttctt cataaagcgc cataaattgg tcaatctgat gagattctgc
    11821 catagataaa gctgtcacca catctgatgt gttgaccacc atgccacgac ccactagaac
    11881 cagtagggct ttg
//
