LOCUS       NZ_CP035435            23767 bp    DNA     circular CON 13-OCT-2024
DEFINITION  Streptococcus pyogenes strain emm64.3 chromosome, complete genome.
ACCESSION   NZ_CP035435
VERSION     NZ_CP035435.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1779766)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1779766)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005161325.1-RS_2024_10_12
            Annotation Date                   :: 10/12/2024 01:25:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,698
            Genes (coding)                    :: 1,645
            CDSs (with protein)               :: 1,645
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 26 of 53
            Pseudo Genes (incomplete)         :: 27 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 13 of 53
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:28:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1212255
            Orig. end                         :: 1236022
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035435.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..23767
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="azole-containing-RiPP"
                     /region_number="3"
                     /rules="((YcaO or TIGR03604) and (((thio_amide and (PF06968
                     or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N
                     or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or
                     PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or
                     thiostrepton or TIGR03602)"
                     /tool="antismash"
     cand_cluster    1..23767
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((YcaO or TIGR03604) and (((thio_amide
                     and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /kind="single"
                     /product="azole-containing-RiPP"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23767
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13767](-)"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..13767)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
     misc_feature    complement(132..143)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.03"
                     /tool="antismash"
     misc_feature    155..169
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.38"
                     /tool="antismash"
     gene            complement(1397..1897)
                     /locus_tag="ETT64_RS06335"
                     /old_locus_tag="ETT64_06340"
     CDS             complement(1397..1897)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /locus_tag="ETT64_RS06335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06340"
                     /product="QueT transporter family protein"
                     /protein_id="WP_020833390.1"
                     /transl_table=11
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRVSEMMN
                     FLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLFNG
                     IFNKAFVYFSFFFATSMLTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKLSQR
                     ISF"
     regulatory      complement(1895..2010)
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            complement(2175..4907)
                     /locus_tag="ETT64_RS06340"
                     /old_locus_tag="ETT64_06345"
     CDS             complement(2175..4907)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /locus_tag="ETT64_RS06340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06345"
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="WP_136097834.1"
                     /transl_table=11
                     /translation="MINKKCIISVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKRE
                     SVISDKSNFPVISPYLASVDFGERKTPLPTPDKGVKVTTEQSVAQVRKGPEERTCTVTG
                     KITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQQVT
                     AHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDDSGKS
                     IVVHIDHRTGVKGTDVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKKVTSSN
                     SDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDGDLATSD
                     GIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKTGTAQVPS
                     PLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGPMKNKEIYV
                     LPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAGPVSYSYGNY
                     KVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSSTKDEKVKRIAE
                     SFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGPTYRYVDIAPEN
                     NVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSVGRIDPTNAAWKD
                     VRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQRRHVLANMLAQFAK
                     EGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRYSYFHQGNNQTLDNI
                     LVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKENDKAESSKQSVKAKKT
                     LKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     misc_feature    5109..5124
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.72"
                     /tool="antismash"
     misc_feature    5230..5241
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 18.98"
                     /tool="antismash"
     misc_feature    5458..5471
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.45"
                     /tool="antismash"
     gene            complement(5478..6596)
                     /locus_tag="ETT64_RS06350"
                     /old_locus_tag="ETT64_06355"
     CDS             complement(5478..6596)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990425.1"
                     /locus_tag="ETT64_RS06350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06355"
                     /product="ABC transporter permease"
                     /protein_id="WP_109828703.1"
                     /transl_table=11
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQAK
                     IGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSDKV
                     LQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQLNY
                     DVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSNLSRF
                     RYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLITFFLFSLLAIGFGLLIGA
                     ITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIAIFQSGG
                     TLSQAWPYLLALMGTALALISFSSLLLKPTKL"
     gene            complement(6593..7720)
                     /locus_tag="ETT64_RS06355"
                     /old_locus_tag="ETT64_06360"
     CDS             complement(6593..7720)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /locus_tag="ETT64_RS06355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06360"
                     /product="SagG family ABC transporter permease subunit"
                     /protein_id="WP_011284655.1"
                     /transl_table=11
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFNT
                     ATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGALTA
                     VLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKRSVD
                     YVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTGVSPS
                     LLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAIGIGLG
                     ISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDSLFYLIF
                     GGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            complement(7729..8652)
                     /locus_tag="ETT64_RS06360"
                     /old_locus_tag="ETT64_06365"
     CDS             complement(7729..8652)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /locus_tag="ETT64_RS06360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06365"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_109828705.1"
                     /transl_table=11
                     /translation="MSFVQLINVVKSYKNGKKAVNDVSMSIEAGNIYGLLGPNGAGKST
                     LINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGSLY
                     GLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDEPTV
                     GIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPKFELE
                     KRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQLTQANI
                     TFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            complement(8675..9358)
                     /locus_tag="ETT64_RS06365"
                     /old_locus_tag="ETT64_06370"
     CDS             complement(8675..9358)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /locus_tag="ETT64_RS06365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06370"
                     /product="SagF family protein"
                     /protein_id="WP_002990431.1"
                     /transl_table=11
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDVL
                     LSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVLIL
                     IFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQSLTS
                     LATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVTYLMI
                     SCKEY"
     gene            complement(9355..10026)
                     /locus_tag="ETT64_RS06370"
                     /old_locus_tag="ETT64_06375"
     CDS             complement(9355..10026)
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /locus_tag="ETT64_RS06370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06375"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_002985272.1"
                     /sec_met_domain="Abi (E-value: 1.1e-10, bitscore: 38.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRAT
                     LIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKKQW
                     LPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSFCYA
                     LNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKKKKEV
                     T"
     gene            complement(10001..11359)
                     /locus_tag="ETT64_RS06375"
                     /old_locus_tag="ETT64_06380"
     CDS             complement(10001..11359)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /locus_tag="ETT64_RS06375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06380"
                     /product="YcaO-like family protein"
                     /protein_id="WP_002990434.1"
                     /sec_met_domain="YcaO (E-value: 6.4e-63, bitscore: 210.1,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 3.9e-111, bitscore:
                     368.2, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKSI
                     TGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRIVY
                     ASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFFEDA
                     EMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSMMLSW
                     YTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILKNKYDE
                     GPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLFLDLDSN
                     VFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKVSPNAVFL
                     DITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHPMP"
     gene            complement(11379..12437)
                     /locus_tag="ETT64_RS06380"
                     /old_locus_tag="ETT64_06385"
     CDS             complement(11379..12437)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03603"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cytolysin_C_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /locus_tag="ETT64_RS06380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06385"
                     /product="streptolysin associated protein SagC"
                     /protein_id="WP_002992154.1"
                     /sec_met_domain="Cytolysin_C_RRE (E-value: 1.5e-40,
                     bitscore: 134.1, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03603 (E-value: 8e-140, bitscore:
                     462.6, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIVY
                     QEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMKIL
                     MGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQTDV
                     SSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIGFVDG
                     PFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQVLPATQDVSQAYLPLLNVLM
                     NLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLKYEDQQI
                     STREIVKKLLDE"
     aSDomain        complement(12168..12437)
                     /aSDomain="Cytolysin_C_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing discrete C protein in a
                     SLS/cytolysin LAP cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_ETT64_RS06380_Cytolysin_C_RRE.1"
                     /evalue="2.40E-42"
                     /identifier="RREFam014.1"
                     /label="ETT64_RS06380"
                     /locus_tag="ETT64_RS06380"
                     /protein_end="90"
                     /protein_start="0"
                     /score="134.1"
                     /tool="antismash"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIVY
                     QEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLML"
     gene            complement(12434..13384)
                     /gene="sagB"
                     /locus_tag="ETT64_RS06385"
                     /old_locus_tag="ETT64_06390"
     CDS             complement(12434..13384)
                     /codon_start=1
                     /gene="sagB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: PF00881"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /locus_tag="ETT64_RS06385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06390"
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="WP_011054324.1"
                     /sec_met_domain="PF00881 (E-value: 5e-32, bitscore: 108.0,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSFFTKEKQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVLK
                     TSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVYES
                     QEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASIRDG
                     ASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEENVRDF
                     AEYGAINAENCNIIIIYVYHYIKNTRKYGNQATAYAFIESGEIAQNIQLTATALTYGSI
                     DIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     misc_feature    13582..13599
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            complement(13606..13767)
                     /gene="sagA"
                     /locus_tag="ETT64_RS06390"
                     /old_locus_tag="ETT64_06395"
     CDS             complement(13606..13767)
                     /codon_start=1
                     /gene="sagA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03602"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /locus_tag="ETT64_RS06390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06395"
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="WP_002985285.1"
                     /sec_met_domain="TIGR03602 (E-value: 1.7e-29, bitscore:
                     98.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQGG
                     SGSYTPGK"
     gene            complement(14537..15844)
                     /gene="eno"
                     /locus_tag="ETT64_RS06395"
                     /old_locus_tag="ETT64_06400"
     CDS             complement(14537..15844)
                     /EC_number="4.2.1.11"
                     /codon_start=1
                     /gene="eno"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /locus_tag="ETT64_RS06395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06400"
                     /product="surface-displayed alpha-enolase"
                     /protein_id="WP_002985288.1"
                     /transl_table=11
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTGE
                     HEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPNKG
                     KLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPIAFQ
                     EFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDGVETI
                     LKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQVDYLEE
                     LVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIKENAANS
                     ILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQIKTGSL
                     SRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     misc_feature    16047..16060
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 24.68"
                     /tool="antismash"
     gene            16077..16535
                     /locus_tag="ETT64_RS06400"
                     /old_locus_tag="ETT64_06405"
     CDS             16077..16535
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /locus_tag="ETT64_RS06400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06405"
                     /product="YueI family protein"
                     /protein_id="WP_002990451.1"
                     /transl_table=11
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQT
                     KTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMTSP
                     FAFILHTDHAINLKETRLEVILKQTKNDQLSKQTPEKTKSFWKRFLKK"
     gene            complement(16667..18391)
                     /gene="ezrA"
                     /locus_tag="ETT64_RS06405"
                     /old_locus_tag="ETT64_06410"
     CDS             complement(16667..18391)
                     /codon_start=1
                     /gene="ezrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /locus_tag="ETT64_RS06405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06410"
                     /product="septation ring formation regulator EzrA"
                     /protein_id="WP_002990455.1"
                     /transl_table=11
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQALF
                     ALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFNFI
                     RAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASISENE
                     DNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITEQIPA
                     IVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELVTLDLD
                     RAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKDEIARLS
                     RKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSIKTLTNVE
                     SGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFLSAFFTTSS
                     QLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQLLQYSNRYRS
                     FEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYEKTREHIRF"
     misc_feature    complement(18611..18629)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.15"
                     /tool="antismash"
     gene            complement(18759..20711)
                     /gene="gyrB"
                     /locus_tag="ETT64_RS06410"
                     /old_locus_tag="ETT64_06415"
     CDS             complement(18759..20711)
                     /EC_number="5.6.2.2"
                     /GO_component="GO:0005694 - chromosome [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gyrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /locus_tag="ETT64_RS06410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06415"
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="WP_002994058.1"
                     /transl_table=11
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGL
                     HHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVVADL
                     EVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGME
                     QEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFA
                     NNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVISVKHPN
                     PQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRA
                     REVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIR
                     GKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIR
                     TLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQLKTALEKYSI
                     GRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPR
                     RDFIEENAVYSTLDI"
     misc_feature    complement(20679..20692)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.9"
                     /tool="antismash"
     gene            complement(20712..21272)
                     /locus_tag="ETT64_RS06415"
                     /old_locus_tag="ETT64_06420"
     CDS             complement(20712..21272)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990461.1"
                     /locus_tag="ETT64_RS06415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06420"
                     /product="HAD-IA family hydrolase"
                     /protein_id="WP_011017599.1"
                     /sec_met_domain="HAD_2 (E-value: 1.2e-16, bitscore: 58.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAVYQKL
                     KESTAIAVATFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNFLISHR
                     DCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDIDSGLVIGDRLIDKQ
                     AGQAAGFNTLLVDGRKNLLEIVT"
     gene            complement(21375..>21645)
                     /locus_tag="ETT64_RS09320"
                     /pseudo=""
     CDS             complement(21375..>21645)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056811.1"
                     /locus_tag="ETT64_RS09320"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="rod shape-determining protein RodA"
                     /pseudo=""
                     /transl_table=11
                     /translation="LIYRMLKVTFKLNHFLYIYFKRIYHIGK"
     gene            <21650..22003
                     /locus_tag="ETT64_RS06420"
                     /old_locus_tag="ETT64_06425"
                     /pseudo=""
     CDS             <21650..22003
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264737.1"
                     /locus_tag="ETT64_RS06420"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06425"
                     /product="tyrosine-type recombinase/integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="KQSANSFPYSKGLPILNAYINKRLKIYGDYHTHLFRHSHISFLAE
                     KGIPLNAIMDRVGHSAPKTTLSIYSHTTVNMKEIINKQTDPFKTGIKQKAFDVKAFSVT
                     IMTPAGIEPATTP"
     tRNA            complement(21966..22037)
                     /anticodon=(pos:complement(1234257..1234259),aa:Arg,
                     seq:cct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="ETT64_RS06425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="ETT64_06430"
                     /product="tRNA-Arg"
     gene            complement(21966..22037)
                     /locus_tag="ETT64_RS06425"
                     /old_locus_tag="ETT64_06430"
     gene            complement(22284..22631)
                     /gene="rplS"
                     /locus_tag="ETT64_RS06430"
                     /old_locus_tag="ETT64_06435"
     CDS             complement(22284..22631)
                     /GO_component="GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rplS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /locus_tag="ETT64_RS06430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT64_06435"
                     /product="50S ribosomal protein L19"
                     /protein_id="WP_002985298.1"
                     /transl_table=11
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFEG
                     VVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRALQ
                     GKAARIKEIRR"
ORIGIN
        1 tcactaccac aagatgggca tagtttagga ataagcatta cttcttgctg gttccgctta
       61 ctcataacga catttaagac agcaggaata atgtcccctg ccttatatac aatcactgta
      121 tcgccaatac ggatatcttt ttcggcaata taatccacat tatgtagggt tgcacgactc
      181 acggttgtcc ccgctagttg cacaggagtt agattcgctg taggtgtgac cacgcctgtt
      241 cgtcccactg tccagtcaac cgaaaggatt tctgcctctt tttcttctgc tggaaatttg
      301 taagcaatgg cccagcgtgg agctttaacc gtaaacccta gctcttcttg catggctagg
      361 ctattaactt taataacaac gccatctatg tcataagcta attgatctct ttttgcctca
      421 atagttttga taaaatccca aatctcatcc atagacgatg tgagttgata atacggattt
      481 actgaaaaac ctagatctgc taattctgct aagacctcat tttgttgatt tctagctgtc
      541 ggactagctt cttgatacaa aaaagtggct agttgacgct tagcaaccac tgacgtatct
      601 agctgcctta gtgtcccagc ggcagcattt ctaggattag caaattcagt ctccccattt
      661 tcttggcgtg cttcattgat cgctttaaag gactgacgag acatgtaagc ctctcctcga
      721 acggtgatgg ttaaaggctc actcaattgg tgaggaatat cctttatttt tttgatattt
      781 tctgtgatat tttcaccgat attgccatca ccccgtgttg ctccaacctg taagaagcca
      841 ttctcataac tcaaggaaat agataaacca tcaattttta actctgctag ataggtggca
      901 ttcggaaatt cggccttgac gcgcttatca aaagcatcca gctcctcacg tgaaaaagca
      961 tcctgcaaac taaaaagcgg atactgatgt cgatattttt caaacccctt taaaatagtc
     1021 cctccaacct gctgcgttgg gctatcttgc aagacatact cgggatatga ctgctctaaa
     1081 gtcaccagtt cacgataaag cttatcatag tcactgtcag aaactgatgg ggcatcttta
     1141 gtataatagt cataacggta acggttaaga agatctgtta attcttttat gcgttttttc
     1201 atacttttat tttatcataa aagataacaa atgagctatt ttctatatca taacaacatc
     1261 aaaagtaatc gttggccact ccatatggta ctattagcta aagtgttaaa tatttctatc
     1321 tagaacgatt ttaacattac aaacaacata aaaaagagaa gggtattgac ccaactcttt
     1381 tgagtatgat gcattactaa aaggaaattc tttgagacaa cttatcaata ataagtgatc
     1441 ctatgagcag agatactaat tctcctaacg cagttgtaaa ccatgttaat aaaaatggag
     1501 ctccaaagaa gtaaagttcg attgctacag taagcatgga agttgcaaaa aagaaagaaa
     1561 agtaaacaaa agctttatta aaaatgccat taaaaagata atctttttga tacttactaa
     1621 acaaaataac acctaaggtc acaaaaataa gagttgaacc gccgcctaca aagacatcaa
     1681 tcagtccgaa actataaaag ttagcaatca tacatccaag agtcaccgca ataatatact
     1741 tgcgatgata aaaagctaag aaattcatca tttcagatac tctaaattga tacatcccat
     1801 aagaaatcgc atttaaaggt ggggtaatag ttaagacaac atacaaggca gctactagcc
     1861 caatgtgaac atagtcgtgt acagtcaatt ttgtcatttt ttctccttta acgtctggat
     1921 aactctcaaa agctatctac gtttctaata tgcttggtga aagggaacta agcaccaagg
     1981 gttgcaaagc aacggttaca ttgtaccata aaacaattag cttgacaaaa gtctttagca
     2041 agtaaagctg tcttattgtt atcttctttt gtgatcaatc ttagttttta tatagtcatc
     2101 cattttagct taaggtctaa cgtaagtata tcaagccttt aacgactctt tatgaactcc
     2161 aattcttcaa atcgctatga ttcctttttg cctttagtca ataataaaat aggcactaat
     2221 aaactagccg ttcctagtag cgttatcaca taaacaagac tatctcctgt ttttggcaac
     2281 agttttcctt ttaaagtttt tttagctttt acactttgtt tagaagactc tgccttatca
     2341 ttttctttag aaaatgataa ttgaagtaac aatggatcat gatctgatgc gcgtccgtga
     2401 gcttccataa atggggaatt cacatgaacc atgtcaaatt cgtagtgatc aagtaaatgg
     2461 cgtgaaacta atatattatc aagggtctga ttattgcctt ggtgaaaata agaataccga
     2521 tctgaaatat catgtcggct caccaagtta accatatcac cttcttcgat taattgaatc
     2581 gtctttgtga attcaaaatc attaaagtca cctagcatca caatattagc ttggtgtttt
     2641 gccccttctt tcgcaaattg tgctagcata ttagccaaga cgtgacgtct ttgctcagat
     2701 ttaaaagtga ctggttgcac acgaccataa agagcattat ccccacgctt agagttcaaa
     2761 tgatttgcaa caacaacgac tttacgacct tggaagataa attctgctgc tagtgattta
     2821 cgaacatctt tccaagcggc gttagttgga tcaattcgac caacactaag gtttaattct
     2881 ccattaaccc aagttagagc atcacgagca ccgccttttg gcttatcaga aaggctgacg
     2941 cgctctggtt gataaaggaa tcctgttcga atattaccac ctggttgacc tccgtcaaca
     3001 ttattttctg gagcaatatc aacataacga taagttgggc cacctagttt tttaatagca
     3061 tcaatgaggc gttgtgcgct ttgtgtcgca tccgttgtcc catcatcagt cggcccatta
     3121 ttatcttgga cttcaattaa tccaataatg tctggagcat tcagatcatg aataaaggat
     3181 tcggcaatcc gtttgacctt ctcatcttta gttgaagaag gattggctga gaagttttca
     3241 atattgtaag aagcaatgct taacttgcta aggtcttttt gcaagtttgt tttttcaggt
     3301 tttagatgac catccattaa acttggcatg tttttgctgt catcaacaaa gaccttgtaa
     3361 ttaccatagc tataagatac tggcccagct aatcttcctt tgtaagagtc tcctgcttta
     3421 ataatttgtt tgcctttttt gaaaagaaca ggaatcacat ctgtgttata agaattagct
     3481 ggaagtaata ctccacctga attatttaac ggtcttgtac tagagccagg taagacataa
     3541 atttctttat ttttcattgg accaaggatt ttagcatcat caaccgccac taacatgcct
     3601 tccattgatt cccaataatc aatagcgtct tcttctggat caaacacacg caagccatca
     3661 ttatcaataa tattggctgg cgcgatacga tctttcccta aaacaagcgg tgatggaact
     3721 tgagctgtcc ctgttttggt cactgcttta gccacaattt gggtgatggt aaggtcagtc
     3781 tgcttacgtt cctcataacc acgaccaaag aattcttcca cttcacctga aatggtcaaa
     3841 acatcgccga cttgaacctt agcgttttta gcaaaaacac gaataccatc tgaagtcgct
     3901 aaatcaccat caccattaag atcttgaaca taaaaatgag tggaatcgtc taaataagtg
     3961 acaacaacct gttctacggt gaccgctttt ttgagaagtg gcgacgtatg actagctcct
     4021 tgaatctcgc ctattttcac aatattacga gatgaagcat cactatttga gcttgtgacc
     4081 tttttaacca cttccaattg ttcaagagaa aacggtctta attgtaactg accatcaaca
     4141 atagacaata tggctgtgag gttaatcaaa tcaccctgac ttattttagt aacaacatca
     4201 gtccctttaa cccctgtacg atgatctata tgaacaacta tacttttacc actatcatcc
     4261 cttacaagga aagatgtgtt gttatattga tcagttgata attctccaac tgtcacgtga
     4321 gataacttaa ctaatgttga gggtgttgtt gtttctaatt ctgatattac tgcttcttta
     4381 acagaagtcg gaaaagataa ctctaacttt ttgtgtgcag tcacctgttg gagttgtaaa
     4441 tcacctttaa agcgagtaag tgtacctgtt aattgaacaa tatctccctt actgtatcct
     4501 aaatcttttt gaggataaac ataaagtcca ataccttcac tatcttgaat ataaaagcca
     4561 tagcctcccc agccattgat cacactcgta atcttgccag taacagtaca ggttctttct
     4621 tcaggcccct ttcttacttg agcaacagac tgttcagtag ttacttttac tcctttatca
     4681 ggtgttggca aaggtgtttt tctctcacca aaatccacac ttgctaggta aggtgatatg
     4741 acgggaaaat tcgatttatc actaataaca gattctctct ttggcctttg tgttacgatt
     4801 tcattggcat aagtcaaatt ttggcgtgaa gtaacttctt caacactagt aagcgtaata
     4861 gctagtgtca acaatgaaac agatattata catttcttgt taatcatgat atctcctttt
     4921 aatgtgatta gtttaagaca acaacaagat tttatttttg aatattaacc tccacctgag
     4981 actatgaatt gggatacact ttctgtttgc attaattata ctatcaagta acacgattag
     5041 atcttttctt ttaatctgtt aagtcaccca attaaagact tgtttgatct gacataacat
     5101 cacaagcacc cctgatagcg aacaagacag ctttatagca ttcgatacat gcttattgta
     5161 acaaaaacat ttcataaaat aaatacgttt tcaaaacttt ttttaataac attagtttct
     5221 atgtttattg aaaacgtttt ttaaaatact ttgacaaccc atttatcaac aaaaacgaac
     5281 tttatagagg ggtcgctttt gataatatgc tctctaaaaa acacagtaac aacagtaaca
     5341 tcgcaatcgc ttttaacaaa agataagtgc actagagaaa atataagcaa aaagcaaccc
     5401 ttttattgca tataagccaa taaaagggct acctggtagt tagctaatta ggaaagtatt
     5461 ttttaacaaa agactaatta tagtttggta ggttttaata ataaacttga aaaactaatc
     5521 aaagctagtg ctgttcccat tagagctaac aagtatggcc aagcctgaga taaggtccct
     5581 ccactttgaa aaatagctat ggctgataaa acccaatttt gtggtaataa cttaccaatt
     5641 gcttgcatat aagaaggcgt aatagacaat ggccataaac agccagccaa cattgaagtc
     5701 ggcattacaa taagattagc taactggcta ctttgttggg aattttgtgt gatagctcca
     5761 atcagcaggc caaagcctat cgctaataaa ctaaaaagga aaaaagtaat caaaagaagt
     5821 gatgtcggaa caaaaaaact aatattaaat acttttaaca aactcagcat aatgacaatt
     5881 tgaatcgtaa aagcaacaaa tcctacacag acgtaactca acatatagcg aaaacgagat
     5941 aagttcgata acattaatcg atggtataac tgactagatt tatctgccaa aataccactg
     6001 taaatcacgc tcgtactacc taacattaga ataagcaaaa atcctgtcgt tgtggaagat
     6061 actgctttgc tacgtgatcg gtctgtcaaa gttacttgct taacatcata atttaactgt
     6121 tggttttttt ggagaattct attaaaagta tcttcgttac ctagagccac atcccctata
     6181 atattataat tttccaatag ataattggtc tgtgccttta cccactctgt aatttcgcta
     6241 ttagcaatcg aacgtatatt gagtttttga gattttcctt gcaacacttt atctgagaat
     6301 cctttgtcaa tagtcaatac cgcttctaga gatttatctt gaaggtagtg atcaatatgc
     6361 tcttttttga ttttggtaaa aatgtcgtac tttttgtttt gctttagttg agcaataaat
     6421 tgtttagata tctgactatt atctttatct aatatcccaa ttttagcctg attattattt
     6481 gaaaaactca aagataaagc caaaagtgtc gttaaaatag gcattaaaaa gaatgtcaca
     6541 taacttgttt ttttgcgctt tatcttaaca aatagagttt ttataaaaca aatcatgtcg
     6601 tcacctgctt tctcatacct atcaatgcca atattataaa aattgttccg atactaatat
     6661 taacgatcaa agttacagga atcggattat attgaccacc aaaaataaga tagaacaaac
     6721 tatcattaac ccatttaata ggagatatat tcgacaattg attaataata gagctgtgta
     6781 atgttgtcaa tggaacgtaa ctgcctccta aaaaggcgaa tataggaata atagtattgg
     6841 atgcaaccgt taaaaaagcc tcatttttaa tgcttatgcc tagcccgata cctatagcta
     6901 cagagagata gactaaggag gctgttatac caataagcat ccactcatta gtgccccaat
     6961 taactcgtaa aacaaacctt gtaaaaatgt ataaaagact caattgaaca gttgttgcta
     7021 acatagcacc tattaatttc ccaaagacca aaagacttgg agaaactcct gttaagtgaa
     7081 tacgatttgt aatccgttgt tgacgatctg acaaaacaag gttcatcgca gacatcatac
     7141 tataaaaagt aatcatggta aacatggaaa tagcataata atcagctgat gtcattcctt
     7201 tacttgtctg tattgaactg accttaacat agtccacact tcgctttctg atatgattta
     7261 attgttgagg ataaagagct gctatcgagt cataagtttt agcattatct acataatttt
     7321 ttactagcat attgatcaaa tctgcatttt gctgattaat attattagta ataaccttaa
     7381 tggtctgatt cttcttcact tctaaaacag ccgttaaggc tccttcactg accctttgtc
     7441 gatctttttc aagagaatta ctaggtttag cctctaaatg aagtttttgg tttaaaactt
     7501 ttaaaaaagc aagaaaattt ttctggtaat ccgttttttc accctctaat tggtaacgaa
     7561 tggtcaattt agggactgtt gtcgcagtat taaaacttga tttaaaggca aaacttaaaa
     7621 taacgatcat caaaattgga aaaatcacca tcatcaataa ggctgttcgg tttctaaaaa
     7681 tctgtaaact ttcttttttg attaaatgaa ataaaaccat agtaacctct aatctcgtaa
     7741 cttcttacct gttaagtgta agaaaatttc ttctaaattc aaatggttat gtctaatctc
     7801 gctaaaagta atattggcct gtgtgagctg atgaacaact gatgtcatat cactattatc
     7861 aatcttcaac atgagttttt ctccatcttc tatcaaagac caatcaggct tatctgccag
     7921 ttccaaatga cgtgaatctg ttagagttac aataatctgg tttgctagat ttgcaacgta
     7981 acgtttttcc agttcaaatt taggtccttc ttcaataact tgaccatgat ccataataaa
     8041 aatataatca caaagagcct ctacttcttc catgtaatgg gtcgtataaa taactgtggc
     8101 accttctttg tttagcaaac gaatcgactc taaaatatga ttacgtgatt gaggatcaat
     8161 ccctacagtc ggttcgtcaa aaatgattaa tttaggtgaa tgaactagcg cacaagcgat
     8221 atttaaccgt ctcttcattc ctcctgagaa ttgacttgga aactgcttag cttgggagtg
     8281 tagccccaca aattctaaac tttttagaac ttgtttttta agctgagctc cctttaagcc
     8341 ataaagtgac ccaaatagtt ctacattttc ataagcagtt aggtctggat aaacagcaat
     8401 gtcttgagga acataaccta tctgcgaact tatttttcga atagtctttt gggattgccc
     8461 taaaacagta attttaccgg aactcaaagg gatcaagcct aagataagat taatcaaggt
     8521 agacttacca gcaccatttg gtcctaacaa accataaata ttacctgctt caatagacat
     8581 ggaaacatca ttgacagctt tcttgccgtt tttgtaggac ttgacaacat ttattaattg
     8641 tacaaaactc atatatcact cctttttaat attgctaata ctctttgcaa ctaatcatca
     8701 aataagtcac cgttaatatc atcgcaccta ctagccaacc atataataaa gttgccgtaa
     8761 ctgcccatag tccaactata gtgaaaagac aaaaagctaa tagtacattt aaccaacctg
     8821 aatagcgaca agccttgact atcccataac ctaaaataag cgttgctaaa cttgttagcg
     8881 actgttctcc taataatata agcaagccca aataaaagag actgtctact agaataacta
     8941 tgcctagtaa aagtatactt aagactggtt tatcttgtct aaaaggcagc caagttattt
     9001 ttttaaagag caatcgactg tctttctgga gcaaaacatc aaagataaga attaaaacta
     9061 aaagcgtcat ctctatgatg gccataagat ataacactaa ataacaaaga atctgccaat
     9121 gccagctagt ttgtgtcaaa aaggtcagaa cctctcctag attaatagta gctgagtaaa
     9181 agaataaaac aagtataata atgacaaatt gaaacagact cagtaagaca tctaaaaaat
     9241 cttgatagga taaggccact aaccattgaa gatgccatct agccaaacac cgtcttcgat
     9301 aactggtcaa ccaaagagta gttagaccta aaaacgacag caaaaccaat agcatcatgt
     9361 cacctccttc ttcttttttg tttgaagatg gctaagacac tcaactagca agttacctcc
     9421 tacatgcatt atcacaacca accacaattg actagtcagc atgtaaatac taccataaat
     9481 gatgcctgtt accaacttag cataaaaaat ggatttcccc atcaataagt gatttaaagc
     9541 atagcaaaaa ctagttacaa ttaaaacaat aattgttggt aaatgccatt gaaaagctaa
     9601 tatattaaac cacaaaaaac gataaatcgt ctcttcaaat aaagctagta ataaaggata
     9661 gccaatctta gaccaatcat ttgtcgttcc tacaaacgag aaagttgcag gaagccattg
     9721 ctttttttgc caacaacgta aaccatgtaa gaaaacgagt tcaatcagta ctatcaagac
     9781 gcaaaataca agtcccataa agaacgtctt actatcaata ctttcaagag acaaggttat
     9841 ttgaggtgac aaaacataaa tacttaccaa agccaaaatg tcataaatca gagttgctct
     9901 tagaaaacca attaaattaa atgcataacc actatcttct tttccaaaaa tagcttgcat
     9961 aggaattgaa ggacaaaaag ttaccaacaa aaaacaaaga ttaaggcatt ggatggacaa
    10021 aggcatttgt tacacctcca aactgtctca ttcgtggatg attagcaaaa ggaaatgctg
    10081 gtatacacat ttcaagaagt tctggcatca gaactcgcgt gacgtaccag cctttttcta
    10141 gagcttcagg aggtgtaata tctaaaaaga cagcattagg actgactttc ttagcataag
    10201 ctaataaagt cttcaaatct tcttttttat ctttaccaga atgatcttcc aaatcactta
    10261 acagaacttc tcctgaaatc aaaggttcaa aggccttcca tttatggtct tggtccttgg
    10321 gatgtgcata atagaaaaca ttactatcta gatctaaaaa caaaggttct tcgcattcaa
    10381 tatttgcaag ggaagctttt tgatataaaa gattatagta gtaactatag ctaatagctg
    10441 aagcttccat gatccctctt agcaaagcat gtttaggatc taacccagcc tgcactccaa
    10501 acaagaggta aggcccttca tcatatttat ttttgagaat gattccaaac gtataaagag
    10561 gattatcttc tcctacagtc atatcgatag gaataatgtc gtatagactg tcttttccca
    10621 gtctagcttc ttctaaaatc acttcaatat caggatcatc aacaataatc ttggggcaag
    10681 gcttcttagt ataccagcta agcatcattg aatcgatctg gatgtattca atcaaactgt
    10741 tgcacatagc cgcttctaac gttttgtgag aagctgttcc cgtagaaaat cctggtatta
    10801 tccgtctttc tcctacagtc tcatttgttt tatacccaac acaaagcatt tgagctggaa
    10861 catacatctc tgcgtcctca aagaacatgg gacatttaac ccagcccaat acatcgttct
    10921 cagttaccat tttatcgcac atcatcattt gcaagtcaca agataatgcg atctgttctt
    10981 gagtaaacac ttgcaagtat tctaaaggca tcactttatg taacagtttt aactcattat
    11041 aagaagcata gacaatccga tctgatagta agtctccggc aattacagta gcatagcgtt
    11101 caatactttc tcctaaatac ttgataagtg cttcttcata gtaactgccg taaccaataa
    11161 tgtgataact aacttgactc aactcaccta taaattgttt atgataatct ggcatttggc
    11221 cagtaatact ttttaagtaa acatcgtgag gatggttgca aactggaact tgagattgat
    11281 ttaagatacc tgttcgattg ccacttaaac tttttaattc atcaaaaata tgattaaaag
    11341 aaggataata gtataacata atagattctc cttataggtt actcgtcaag gagtttttta
    11401 acaatctcac gtgttgatat ctgctgatcc tcatatttta attttgccaa agctccttgt
    11461 gtctgtgaat tagacatttt caaaatgtct tggacctgaa tctcaagcgt cggtaagtaa
    11521 atgcttaata aacgtccctc aaatttagaa ctacctgttt gagcaataat gaaagcttcg
    11581 ctaacgacta aattcattaa aacatttaac aatggtaaat aggcctggct cacatcttgt
    11641 gttgctggta aaacctgatt agcaaaatgt tgatagagag ttgagtcttg taaacgtgcc
    11701 aaaactcttc tttctaagct atcaaagtca gcactatggg gaggattcaa agtacaagtg
    11761 tgcaaaaatg gtccatctac aaaaccaatc actaattgtt ttttcatagc tacgctaact
    11821 tcattcaagt gtcgcaacat cataatattc aagcgttctt gacaaataat aatactttgg
    11881 taatcgcaca aggcttctga taaacaattc atgttacggc gatgctccaa agcatctaaa
    11941 cgcgaactaa catctgtctg ctgaattaac atttttaagt catcgctagc cacttgcaat
    12001 tgtaaatgca gttgttcagc tagcaactca gcagattcat taacataagt cgaatcactg
    12061 ataaacaaaa caggatcatt gcttaccata tgacctgctt gcgccataaa acggaaatta
    12121 cccattaaaa ttttcatgac attttcttca agattgtagt catcctcaag catcagcata
    12181 tcattgtaat acaagcctga aatgacttcc attaaattag cgaacgtctc aggttctaaa
    12241 gtattttcat aagtttcagt atcaatggct actcccttaa ccaactgtga aactatttct
    12301 tgataaacta tctttaaatt ttgaggttct tgtgtcaaat ctagaatggc ttcattaaag
    12361 tcccagattc cttttctaaa acaaaatgta tcttgaaatg tcacgacacg aacattacta
    12421 ttaagttgat atttcattga gactccttag ttcctacgag tgtcatgtga atcacatgct
    12481 ctcctagccc atctaaatct aataattctt ggagatattc cttattataa ccaccaatat
    12541 caatacttcc ataagttaag gcagttgcag tcaattgaat attctgggct atttctcctg
    12601 attcaataaa agcataggca gtcgcctgat tcccatattt acgtgtattt tttatgtaat
    12661 ggtagacata aataataata atattacaat tttcagcatt aatagcaccg tattccgcaa
    12721 aatctctaac gttttcctca gagctatgcc gataacacct tagtgcatgc tgatagggta
    12781 gatattcata gaaaccatct attaatttac tgatgtttct agcataaaaa actaaatgaa
    12841 tagggtataa acctccacct gaagcacagt ttctgagtgt aatcttatct gatgctccat
    12901 ctctaattga tgcttgtgaa ctaacaccgc atgcataata aagaatgttt gataaatctt
    12961 gaagaggcat ttgtctatcc gaaaattggc gatgacttct tcgttttata atacatgtcg
    13021 acagagcagt tgatatcttg gtaggttttg gtaaacgaat tatcttttcc tgactttcat
    13081 aaacgtaact acttgaaaaa ttagcaacgg cagaatctgt aaaaaagtcg acaatactag
    13141 cttggaagcc taaataatta ttattagctt tatagttcat caaaaaatgt tgactaagat
    13201 tatcggttgc attaggcatt aaatgttgag caactagctg ctttgacgtt tttagcaccg
    13261 tttgatgatg gtaatccgat aaggacaagt ggtttgtatt aaattcaaac aattgccttg
    13321 ctttttcaac agttattggc ggacaattct ctttaggttg tttttccttt gtaaaaaatg
    13381 acataactat ctttcctatc tcattttact aaacatatag taattagcag gtactgtcta
    13441 gtacctgcta attacctgag acgttagcat caagaagatt acttttgtct catgttttct
    13501 aacacctcta tgccattcat ctataatcag aagttaccaa agataactta gttgctagca
    13561 aactcattac cctaatatca ctatcacata gagatgctaa atagattatt tacctggcgt
    13621 ataacttccg ctaccacctt gagaattacc acttccagta gcaattgaga agcaacaagt
    13681 agtacagcag caacagcagc ctccaggagc aacttgagtt gtttcagcta cactagtagc
    13741 taaaatattt gaagtaaatt ttaacataag gtttacctcc ttatctaata agtaactgat
    13801 aagaacacga gtcgtttatt tttaacctta ttttaaaaat aaagttaaca caacaaggac
    13861 aaggctagct caattgtacc attgtaacac aacaactatc tagttcttat cacttttatt
    13921 atagtaaaaa atgattaata tgtaaaccct ttctttttaa tattatcaaa aatgacaaaa
    13981 aaaataataa ttttctctaa aaaactatat ttttcaaaca atagcgacaa aaatgagagc
    14041 taattttcaa agctttataa cttatatcat tttgataacc ctagctaata tctttacttc
    14101 atcctagata ctactttgaa atatgtaata cctggacaaa cctataccac gacgttttta
    14161 ctaaataaaa aaactttggt cctgtattat cagaaccaaa gttttttaac aagttattaa
    14221 gtttttctca cgaatcatta actcaggata aattagtcca cagtaaattt tcactattta
    14281 gaaatcaaaa agcttgggat tttcccaagc tttctattct ttaacttttg cgtcgtagct
    14341 ggctggatgt tcactccttt tgtcgtgacc aacctaatca gcctgacgga ggtgcgacga
    14401 cgaaatcgca ttctacgaat gaccgatttc tgtcttactg caaaaagaaa agacttggat
    14461 aacatatcca agccttcctc aataacttta cctcgctaat acattagctc aggataaatt
    14521 agttcactag actaatttat ttttttaagt tatagaatga tttgatacct ttgtattgag
    14581 caacttcacc aagttgatct tcgatacgaa gtaattggtt gtatttagca atacggtctg
    14641 tacgtgacaa tgaacctgtt ttgatttggc cagcgtttgt tgcaactgcg atgtcagcga
    14701 ttgttgaatc ttcagtttca cctgaacggt gtgatacaac ggcagtatat ccagcttctt
    14761 tagccatttc gatagcttca aacgtttcag tcaaagtacc gatttggtta actttgataa
    14821 ggattgagtt agctgcattt tctttgatac cacgagcaag gtattcagtg tttgtaacga
    14881 agaagtcgtc accaaccaat tgaacacgtt tgcctaggcg ttcagtaaga actttccaac
    14941 catcccagtc gttttcatcc ataccatctt cgatagtaat aattgggtat ttgttaacca
    15001 actcttcaag gtaatcaact tgttctgcag atgtacgaac agcagcgcct tcaccttcaa
    15061 atttagtgta gtcgtaaact ttacgttctt tgtcgtagaa ttctgatgat gcacagtcaa
    15121 aaccgatcat aatgccgttt tcaccagctt cgtaaccagc tgcttcaata gctttaagga
    15181 tagtttctac accgtcttca gttccttcaa atttaggagc aaatccacct tcgtcaccta
    15241 cagctgtaac aagtccgcgt tctttaagaa ttttcttaag agcgtggaaa acttcagcac
    15301 cccaacgaag accttctttg aaagtaggtg caccaactgg catgatcatg aactcttgga
    15361 atgcaattgg ggcatctgag tgtgatccac cgttgatgat gttcatcata ggagttggaa
    15421 gaactttagt gttgaatccg ccaaggtaag tgtaaagtgg cacttcaaga tagtcagcag
    15481 ctgcacgagc aacagcgata gaaacaccaa gaatagcatt agcaccaagt ttgcctttgt
    15541 taggagtacc gtcaagagcg atcattgcac ggtcgatagc ttgttgatcg cgaacatcgt
    15601 aaccgatgat tgcctcagcg ataatgttat taacgttatc aactgctttt tgagtaccta
    15661 gaccaaggta acgagatttg tcaccatcac gaagttcaac ggcttcatgt tcaccagttg
    15721 aagctcctga aggaaccata ccacgtccga atgcacctga ttctgtataa acttctactt
    15781 caagtgttgg gttaccgcgt gagtcaagga cttcgcgagc gtatacatca gtaataattg
    15841 acattgtaat actctcctta tgagttttaa tattttacat gttcataata ccacaaacta
    15901 ggcctttaat caacttaaaa agcaatctca tgaaaagctt cctttgtttt ttgcaagaaa
    15961 aatcactttc tttcttattt tttaacacat ttaccgaaaa ttttcaaatt ggtattttat
    16021 tgtagaaatc gatataataa agaaactatt attattataa agaagcgagg gtatctatga
    16081 ctaatttaga agataagcta ttaaaaggag cctggggaga aagaagatta aatcctgagc
    16141 agcaacgcta ctatctagga acgtatgctg aacgagtcgt tttaagtgct ttattagacg
    16201 aagctcaaac taaaacagta aaagactatt tgaaaagaga attaccgagc cttcagttag
    16261 tctatcaacc tttacactta aaaatctctt ccaagctggc acctcaactt caaatactct
    16321 atatgaaatt agcaaaagaa aaccatttac ctgttactat tattacagaa acgcatatga
    16381 cttcaccttt tgcgtttatc cttcataccg accacgcaat aaatctaaag gaaacgcgtt
    16441 tagaagtcat cttaaagcag acaaaaaacg atcaacttag caaacaaact cctgaaaaaa
    16501 ccaaatcctt ttggaaaaga tttttgaaaa aataactatc ctctcaatag caaaaaacac
    16561 tgttactact tcttcttttg atcattaaaa agaagattct agtcaaaaac acgtattggt
    16621 ccaaaaagct aataaaaagt cgtgagaata tcacgacttt caagtatcaa aaacgaatgt
    16681 gctctcgtgt tttttcataa gagttaacaa accgatctgt taccccaggt tcaactgttt
    16741 caagagcata agaaatttca tcaaaagaag cctgataatc attttctact tcgaaaagtc
    16801 tcaaagcatg ttcaaaacta ctttgaactc ctgcttcaaa agaacgatag cgattagaat
    16861 attgtagtaa ttgttcagtc aaggtagcat tttgaaccac ttgataggtt aaatcttcta
    16921 gattagcaat agcaaccgtc gcaacttccg acaaacgtga cactgcctca atattgatcc
    16981 gtcctctact aagctcatcc atcaaagctt ccaattgtga actcgttgta aagaaagcac
    17041 taagaaagtc ttgtggaatt ccaggcaagt gacgtttttc catataacgt ttaatcatat
    17101 gaagctgagt aacataaaca tcaaggttat ggcgtgcctg tgattcaatt ttttcaatat
    17161 ctttaactgc tgcaaaaaca tccatttgac ctgactcgac attagtaagc gtctttatac
    17221 tgcgttcaaa agtcacttgc agttctgaaa atggtttttc ttgtaaacca aattgttctg
    17281 caacagcaag tgtactgtct tcgatctctt taatatcctt ctcaaatgcc ttaacagtca
    17341 gactttccgt ttcacttaga atatatttac gtgataaacg tgcaatttca tctttgagtt
    17401 gttcattatt acgtttcaca tgtgctaggt aacgaggtag catcttacta tttttagcag
    17461 ctaccttata agcggcaatt tcgcgctcaa agacttcata aagagaatca atgcgttctt
    17521 gaatatgcgt gttttcttct ctagcacgat ccaagtcaag tgtaactaac tctgatgaat
    17581 tggcacgaat agactcacga atctcctgaa aacgtgcctc aatgtttttt tctggaaaat
    17641 gatagttttc ttcaagtaaa cggcgatatc ctgtttccaa atcatctaac tgatctggaa
    17701 aatcatcttc caacttggcc acaatagctg gaatctgctc tgtaatctgc cctagagcaa
    17761 tagtatgctc ttctgctcta tccagaactt cagaagcttc tacaggatca cctgatgagt
    17821 tgagtgcgac aaactgtgaa aattcagttt caatgttttt catctgtttg tcaatttcag
    17881 gcatagttga accaaaatta tcttcattct cagaaataga agcttggagc ttttcatata
    17941 aatcaagagc atgggtgaca cgagcactat ttttttcttc ttgctctttt aagatattaa
    18001 gtgcctcacg aatagaagcg atatcttctt caacaagatt aagctgactt tcaacgctat
    18061 taatctcatg cttggcgcga ataaaattaa acgtatcatt caaattttca gcttcaaaaa
    18121 tatgattttc aatatctgca aacgaattta cagttaagtc tacccatttc tgattccatt
    18181 cccgaaaaga ggtttggctc tgtcctatca aatgaagtga ctttacttct tcaatttcat
    18241 cattaacagg caaagcaaac aaggcctgtt ttcgttcttc tagactggta attagggaat
    18301 cattacgctt tcgtatgatg acacccacta gataggcgat aatcactagt aggactatag
    18361 ccacaatcag caagataatt ccgcttgaca tctgaatctc cttattattt agacacgatt
    18421 tatcaacatt gttccagtaa taactaaaaa ctgaaattcc tcaatcgcct tttattaaaa
    18481 ttttatttaa aatacactat aacattacga ttataacata ctttaaaatg catttcaaaa
    18541 tatatttgac taattcaaca atctcgtacc agcgactcaa aaggctttct taataaggac
    18601 taacataggt attaaggggc ggtctcaata cctatgcatt aacgctgtcc ctcaagatga
    18661 agacgtacta actaacaaag ctgttggcat catgcatcat gatttttagt caattcgtcc
    18721 tctttggtag cagattcctt gtgaaactaa aaaggtgact aaatatccag tgtactataa
    18781 accgcatttt cctcaatgaa atcacgtctt ggttcaacac gatctcccat taacatatca
    18841 aatactttat ctgcttctgc ggcatcatca actgtcacac gcgccatcaa acgattttca
    18901 ggatccatag tagtttccca aagttgatgg tcatccattt ccccaagacc tttataacgt
    18961 tgaacagttg gttttgaacg accaatacta tatttttcaa gagctgtttt taattggtct
    19021 tcttgatcaa tacctggctg aatatactct ttaatctcac taccgacctt aacaccataa
    19081 ataggtggct gggcgatgta aacatagcca gcttctagaa caggtctcat aaagcggtaa
    19141 atcaaggtta aaagtaaggt tctaatatga gcgccatcca catcggcatc ggtcatgata
    19201 accagctttt ggtagcgagc ttttgacacg tcaaaatctg caccaaaacc tgtacccata
    19261 gcggtaaaga gacttctaat ttcttcgttg gcaagaatct tatccatagt tgctttttcc
    19321 acgttcaaaa ttttaccgcg aataggcaag atagcttgaa actctcggtt acgacctgat
    19381 ttggccgacc cacccgctga atctccttcg acgataaaaa gttcgttttg gttagcgtca
    19441 tttgacgaac agtctgctaa ttttccaggt aagtttgaaa tttctaagcc tgattttttg
    19501 cgggtgactt cgcgggctcg cttagctgca attctagctt tagaagccaa aatccctttt
    19561 tccacaatct tacgagcaac ttgtgggttt tccaaaagaa aacgttgaaa ggcctcacta
    19621 aagagacgat tagtgatctt aaccacttct gagttgccca attttgtttt ggtttgacct
    19681 tcaaattgag gatttggatg cttaacagaa attaccgccg tcaaaccttc acgaacatct
    19741 tctcctgtca aattgtcctc attttcttta agaattttat ttttcttagc gtagtcattg
    19801 atgacccgag taagagccgc tctaaagcct tgttcatgcg ttccaccttc atgagtatga
    19861 atattattag caaaactcat gactgtttct tgatagctag tcgtgtattg catggctact
    19921 tcaactgcaa taccttctaa ttcaccatct gtatagatgg gcgtttcaaa gataacatct
    19981 tttttatcat ttaaaaattc aacataagaa ccaattccac cttcataatg gaaatgttct
    20041 tcttgttcca tacctgagcg cttatctgta atggaaattt ttaaaccacg gtttaaaaag
    20101 gctaactctt gaatacgttt tgctaaaaca ctgtaatcaa actgagtcgt ttcggtaaaa
    20161 atttctggat cgggtgtaaa gtgtaccgtt gttccagtca catcagtggt tccaatgacc
    20221 tcaagatctg ctacaacagc cccgcgttta aattcttggt aatgaatttg gccgttttta
    20281 taaacacgta catctaattg tgttgataaa gcattaacaa cagatgaccc tacaccatgt
    20341 aatcctccag aaaccttata gccgcctcca ccaaatttac cacctgcgtg taagactgta
    20401 aaaactgttt caacggcggg acgtcctgtc ttggcttgga tatcaactgg aattccacgg
    20461 ccatcatcta ctactgtaat ggaattatct gcttcaataa agactttaat atgagaggca
    20521 aaacctgcta atgcttcgtc aattgagttg tcaacaattt cccagactaa atgatgcaaa
    20581 ccctctttag ctgtcgagcc aatatacatc cctggacgca tgcggacagc ctccagccct
    20641 tctagaacct gaatttgact ggcatcgtat tcttgcattt ttttttcaaa atgtttattt
    20701 tcttcaatca tctacgttac tatctccaat aggttttttc taccgtcaac aagcaaggta
    20761 ttaaaacccg ctgcttgtcc tgcttgttta tcaattagcc gatctccaat tactaatcca
    20821 ctatcaatat catacttttc ttttaaataa aacaaacttt caggatttgg tttccgagca
    20881 aagccatttg aagctgtcac gacttctgta aaatagatta gcaacccagc ttgctctaaa
    20941 agttggttca cctgacaatc tcgatgagaa attaaaaaat ttcgcgaacc tgaagtcgct
    21001 atcttttcca aaatttcttt agctcccaaa caccaaatgg gttgtgccaa tttgtctgct
    21061 tctcttagcc tatacacatg caaaaattct ggctcattag gagcaaacgt tgccacagcg
    21121 atagcggttg attcttttaa tttttggtaa acagcatcgt gatctcctgg aaggctgaaa
    21181 aaagctagcg tttggacaaa agcttgggtt gaaagctcgt aattatcgag taaggtacct
    21241 cccaaatccc atatataatc ttgataattc atacctttca ttatatcaca aattcactta
    21301 atattatagt tatttcccaa ataaaacagg tatcgtaatg actgaataaa cgagtcttgc
    21361 gatgttctat tcctctaatg gtaacacata cacctcttta gctgttcctt tcctttttga
    21421 tctccagaaa gtatgtactg ataattcata aacagtacca agcccactct aatcaagtta
    21481 gaaatcaaag ctgatcttct ttaggagaca aaaggcaggg aaattcctgt caatagtaaa
    21541 atgccaatag aagcaccgct attttccaat atgataaatc cttttgaaat atatgtataa
    21601 aaaatgattt aatttaaatg tcacttttaa catacgatag attaatcaca agcagagtgc
    21661 taacagcttt ccatattcaa aaggtttacc aattttaaac gcgtacatca ataagcgatt
    21721 gaaaatttac ggagattatc acacgcactt atttagacac tcgcatatat catttttagc
    21781 agaaaaaggg ataccgctaa atgcgataat ggatagagtt ggtcacagcg ctccaaaaac
    21841 aacattatct atttacagtc acacaactgt aaacatgaaa gaaattataa ataaacaaac
    21901 tgaccctttt aaaaccggaa taaaacaaaa agcctttgat gtaaaggctt tttcagtgac
    21961 aataatgacc cctgccggaa tcgaaccagc aactactcct taggagggag ttgttatatc
    22021 cattgaacta aggggtctat cttatattca ccttgtcttc tattttaacc tagaaaataa
    22081 ggtaatgcaa gatgaaatat cagataactg aaagtttatt ttgcttcatt ttgttattac
    22141 aatatactaa ttataactgc taaggaactt tcaaagtgtc ctacgagcat agcgttaatc
    22201 accaattgtc acattgtcac gacgacataa aaagactaga gactctagtc tttttattct
    22261 attacgttac gtttcggttc aaactaacga cgaatttctt tgatacgagc tgctttacct
    22321 tgtaatgcac gcaagtagta aagtttcgca cgacggactt taccgtggcg gataacttcg
    22381 attttgtcaa cacgtggtgt gtggattggg aatgtacgct ctaccccgat accacctgag
    22441 attttacgaa cagtgtacat ttctgagatc ccttgacctt tacgtgaaat aacaacacct
    22501 tcaaagatct ggatacgttc gcgagttcct tcgacaactt ttgcgtgaac gcgaacagtg
    22561 tcaccaggac ggaagttagg gatatcagag cgaagttgac cttctgtcaa actttggatt
    22621 aatggattca tttttattct cctatcttac taatcttaag tacttgtctt agcggattag
    22681 ccgtaatttt cgtgtgtcca ttacacacga gatttattct aacaaaaagc ttctttattg
    22741 taaagctatt tttttaaatc catctgattt accgtcatgt cttttaaatt gatcatgact
    22801 actttttgat aactgtaaat ggaatgtctg tgactaacca tataggctgc agccgaggtg
    22861 acaaaatatg ctagggcatt ctctggacca aagacctcta tcccaataaa gataggggcc
    22921 caaaaggtat ttgttgcact tccaaatacc gtggtgtaac caagtgcagc aaccaacagt
    22981 acaggaagac ctaagtaagg ggccaaaacg atgcctaaag aagctccaat agcaaataga
    23041 ggcgtcactt caccaccttg aaagcctgca gataagctaa tcaccgtcac gatcattttt
    23101 aaaagccagt cgtaagttag gatagtttgg ccagaaaaag ctgccgcaag caaatgagtc
    23161 cctagaccac tatatcttcc aacgtgacca atcatcaaac agatagataa caaaacacca
    23221 atgaaagcaa tcctgagata aggatttggt agcttttgac tcaggtaagg tttaaaccag
    23281 ccaagtaaat aggcaaaact atttcccacc agaccaaaaa taagacctaa cccaattaat
    23341 ttaactaagg ttaagggagt tatggtcaat gtttcttcaa gaacaatcgt aaatttctct
    23401 aatcccaaag aatgagaggt ccaagaagcc acgcaagcag caactaagga aggtaataaa
    23461 gcgcagtagc gaagcgttcc taccaacaaa acctcaagag caaatacgac agcagcaatc
    23521 ggcgtttgaa aaagtcctgc aaaaccggct gccatcccca taatcaaaag gtgtcgtgcg
    23581 gcttcttgac tagtcacaaa tctccgacaa taatgagaga tggtagctcc tatttgaaca
    23641 gctacccctt ctcgacccgc actcgctcca aataagtggg ttacccaagt tgagaacaaa
    23701 ataagaggaa tgagaatagg aggaatctga tctttttgac catgccctac ctcaaaaacc
    23761 aatccca
//
