Select genomic region:
Overview
1.1
1.2
1.3
1.4
1.5

### Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP035435.1 (Streptococcus pyogenes)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | terpene-precursor | 604,681 | 625,553 |
Region 2 | T3PKS | 1,074,619 | 1,115,794 |
Region 3 | azole-containing-RiPP | 1,212,256 | 1,236,022 | High | streptolysin S | ribosomal:RiPP:LAP
Region 4 | RiPP-like | 1,375,898 | 1,387,790 |
Region 5 | lanthipeptide-class-ii | 1,510,740 | 1,533,574 | High | salivaricin A | ribosomal:RiPP:Lanthipeptide

NZ_CP035435.1 - Region 1 - terpene-precursor
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 604,681 - 625,553 nt. (total: 20,873 nt) Show pHMM detection rules used
terpene-precursor: (PT_FPPS_like and not PT_HPP)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
binding site
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
Tailoring
MIBiG comparison
KnownClusterBlast
SubClusterBlast
Terpene
TFBS Finder
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Tailoring functions
Tailoring enzymes detected in this region
Ligases: synthetase
ETT64_RS03210: Polyprenyl synthetase

smcogs
  SMCOG1182: Polyprenyl synthetase

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | terpene-precursor | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0002397.2 |  | 0.47 | terpene | astallatene | Arabidopsis thaliana
BGC0002400.2 |  | 0.44 | terpene | boleracene | Brassica oleracea var. oleracea
BGC0002698.2 |  | 0.40 | ribosomal | phaeornamide | Pseudophaeobacter arcticus DSM 23566
BGC0002398.2 |  | 0.38 | terpene | (-)-ent-quiannulatene | Arabidopsis thaliana
BGC0000487.5 |  | 0.36 | ribosomal | carnocyclin | Carnobacterium maltaromaticum
BGC0000542.3 |  | 0.33 | ribosomal | paenicidin B | Paenibacillus terrae
BGC0000541.5 |  | 0.33 | ribosomal | paenicidin A | Paenibacillus polymyxa
BGC0000502.5 |  | 0.33 | ribosomal | cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7 | Bacillus cereus
BGC0001859.4 |  | 0.32 | other (phosphonate) | fosfomycin | Pseudomonas syringae
BGC0000617.5 |  | 0.32 | ribosomal | coagulin | Bacillus coagulans

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0002397.2 |  | 0.47 | terpene | astallatene | Arabidopsis thaliana
BGC0002400.2 |  | 0.44 | terpene | boleracene | Brassica oleracea var. oleracea
BGC0002698.2 |  | 0.40 | ribosomal | phaeornamide | Pseudophaeobacter arcticus DSM 23566
BGC0002398.2 |  | 0.38 | terpene | (-)-ent-quiannulatene | Arabidopsis thaliana
BGC0000487.5 |  | 0.36 | ribosomal | carnocyclin | Carnobacterium maltaromaticum
BGC0000542.3 |  | 0.33 | ribosomal | paenicidin B | Paenibacillus terrae
BGC0000541.5 |  | 0.33 | ribosomal | paenicidin A | Paenibacillus polymyxa
BGC0000502.5 |  | 0.33 | ribosomal | cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7 | Bacillus cereus
BGC0001859.4 |  | 0.32 | other (phosphonate) | fosfomycin | Pseudomonas syringae
BGC0000617.5 |  | 0.32 | ribosomal | coagulin | Bacillus coagulans

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.

No significant matches found.

Similar subclusters
Shows sub-cluster units that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.

No significant matches found.

Detailed terpene predictions
Detailed information for terpene domains. Shows possible products of a terpene cluster and lists the subtype and expected reactions for each terpene-related domain.
Product predictions:

Class | Subclass | Product name | Chain length | Initial cyclisations | Functional groups
--- | --- | --- | --- | --- | ---
sesquiterpene precursor |  | FPP | C15 | acyclic | PP

Genes with terpene domains:
ETT64_RS03210: PT_FPPS_like
Domain 1 (25 - 272): Prenyltransferase; Farnesyl diphosphate synthase like
Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:
  DMAPP, IPP → FPP

Transcription Factor Binding Sites
Detailed information for Transcription Factor Binding Site hits, including surrounding genes where applicable.
The strand of the match is shown by an arrow underneath the match.
Detailed documentation is available here.
Binding sites found:
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 25.77 out of 26.06
Confidence: strong
ANR
Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.93 out of 26.33
Confidence: medium
RutR
Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.00 out of 26.85
Confidence: medium
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.40 out of 26.06
Confidence: medium
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.67 out of 26.06
Confidence: medium
Additional weak hits (1):
MntR
Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.95 out of 39.62
Confidence: weak
Terpene
TFBS Finder
Glossary
Glossary showing acronyms and their full names.

Acronym | Full name
--- | ---
DMAPP | Dimethylallyl diphosphate
FPP | Farnesyl diphosphate
IPP | Isopentenyl diphosphate
PP | Diphosphate

TFBS definitions
Shows descriptions for Transcription Factor Binding Site models

Regulator | Description
--- | ---
ANR | Anaerobic transcriptional regulator
MogR | Repressor of flagellar motility genes
RutR | Regulator of the pyrimidine and purine metabolism

NZ_CP035435.1 - Region 2 - T3PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,074,619 - 1,115,794 nt. (total: 41,176 nt) Show pHMM detection rules used
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
binding site
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
Tailoring
MIBiG comparison
KnownClusterBlast
SubClusterBlast
TFBS Finder
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: reductase
ETT64_RS05775: hydroxymethylglutaryl-CoA reductase

smcogs
  SMCOG1187: hydroxymethylglutaryl-CoA reductase

Hydrolases: amidase deformylase esterase
ETT64_RS05680: amidase

smcogs
  SMCOG1105: amidase

ETT64_RS05815: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

smcogs
  SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

ETT64_RS05820: peptide deformylase

smcogs
  SMCOG1275: peptide deformylase

Ligases: synthase
ETT64_RS05770: hydroxymethylglutaryl-CoA synthase

smcogs
  SMCOG1043: hydroxymethylglutaryl-CoA synthase

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | T3PKS | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0002561.3 |  | 0.44 | other (other) | phenazine SA, phenazine SB, phenazine SC | Streptomyces sp.
BGC0000934.4 |  | 0.41 | other (phenazine) | 5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G | Kitasatospora sp. HKI 714
BGC0001080.5 |  | 0.41 | other (phenazine) | endophenazine A, endophenazine B | Streptomyces anulatus
BGC0000286.3 |  | 0.40 | PKS | viguiepinol | Streptomyces sp. KO-3988
BGC0002284.3 |  | 0.39 | other (other) | corynecin III, corynecin I, corynecin II | Rhodococcus sp. H-CA8f
BGC0002362.3 |  | 0.38 | PKS | loseolamycin A1, loseolamycin A2 | Micromonospora endolithica
BGC0001863.4 |  | 0.38 | ribosomal, terpene | bacillicn CER074 | Bacillus mycoides
BGC0001521.3 |  | 0.38 | NRPS | auriculamide | Herpetosiphon aurantiacus DSM 785
BGC0001364.3 |  | 0.38 | ribosomal | cinnamycin B | Actinomadura atramentaria DSM 43919
BGC0000679.5 |  | 0.38 | terpene | diazepinomicin | Micromonospora sp. M42

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0002561.3 |  | 0.44 | other (other) | phenazine SA, phenazine SB, phenazine SC | Streptomyces sp.
BGC0000934.4 |  | 0.41 | other (phenazine) | 5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G | Kitasatospora sp. HKI 714
BGC0001080.5 |  | 0.41 | other (phenazine) | endophenazine A, endophenazine B | Streptomyces anulatus
BGC0000286.3 |  | 0.40 | PKS | viguiepinol | Streptomyces sp. KO-3988
BGC0002284.3 |  | 0.39 | other (other) | corynecin III, corynecin I, corynecin II | Rhodococcus sp. H-CA8f
BGC0002362.3 |  | 0.38 | PKS | loseolamycin A1, loseolamycin A2 | Micromonospora endolithica
BGC0001863.4 |  | 0.38 | ribosomal, terpene | bacillicn CER074 | Bacillus mycoides
BGC0001521.3 |  | 0.38 | NRPS | auriculamide | Herpetosiphon aurantiacus DSM 785
BGC0001364.3 |  | 0.38 | ribosomal | cinnamycin B | Actinomadura atramentaria DSM 43919
BGC0000679.5 |  | 0.38 | terpene | diazepinomicin | Micromonospora sp. M42

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.

No significant matches found.

Similar subclusters
Shows sub-cluster units that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.

No significant matches found.

Transcription Factor Binding Sites
Detailed information for Transcription Factor Binding Site hits, including surrounding genes where applicable.
The strand of the match is shown by an arrow underneath the match.
Detailed documentation is available here.
Binding sites found:
CodY
Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.77 out of 27.78
Confidence: medium
LexA variant 2
Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.20 out of 24.78
Confidence: medium
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.60 out of 26.06
Confidence: medium
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.30 out of 26.06
Confidence: medium
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.93 out of 26.06
Confidence: medium
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.33 out of 26.06
Confidence: medium
Additional weak hits (1):
PerR
Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 22.77 out of 28.14
Confidence: weak
TFBS Finder
TFBS definitions
Shows descriptions for Transcription Factor Binding Site models

Regulator | Description
--- | ---
CodY | Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 2 | Repressor of DNA damage response
MogR | Repressor of flagellar motility genes

NZ_CP035435.1 - Region 3 - azole-containing-RiPP
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,212,256 - 1,236,022 nt. (total: 23,767 nt) Show pHMM detection rules used
azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
binding site
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
Tailoring
MIBiG comparison
KnownClusterBlast
SubClusterBlast
TFBS Finder
Azole RiPPs
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Tailoring functions
Tailoring enzymes detected in this region
Hydrolases: hydrolase
ETT64_RS06415: HAD-superfamily hydrolase, subfamily IA, variant

smcogs
  SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | azole-containing-RiPP | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000566.5 |  | 0.99 | ribosomal | streptolysin S | Streptococcus pyogenes M1 GAS
BGC0000564.5 |  | 0.69 | ribosomal | clostridiolysin S | Clostridium botulinum A str. ATCC 3502
BGC0001170.5 |  | 0.64 | ribosomal | clostridiolysin S | Clostridium sporogenes ATCC 15579
BGC0001171.5 |  | 0.50 | ribosomal | listeriolysin S | Listeria monocytogenes serotype 4b str. F2365
BGC0000569.5 |  | 0.49 | ribosomal | plantazolicin | Bacillus velezensis FZB42
BGC0001173.5 |  | 0.48 | ribosomal | plantazolicin | Bacillus pumilus ATCC 7061
BGC0000605.5 |  | 0.42 | ribosomal | GE37468 | Streptomyces sp. ATCC 55365
BGC0002700.2 |  | 0.41 | ribosomal | bacinapeptin | Bacillus nakamurai
BGC0001887.3 |  | 0.39 | ribosomal | huazacin | Bacillus thuringiensis serovar huazhongensis BGSC 4BD1
BGC0002111.3 |  | 0.39 | ribosomal | andalusicin A, andalusicin B | Bacillus thuringiensis serovar andalousiensis NRRL B-23139

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000566.5 |  | 0.99 | ribosomal | streptolysin S | Streptococcus pyogenes M1 GAS
BGC0000564.5 |  | 0.69 | ribosomal | clostridiolysin S | Clostridium botulinum A str. ATCC 3502
BGC0001170.5 |  | 0.64 | ribosomal | clostridiolysin S | Clostridium sporogenes ATCC 15579
BGC0001171.5 |  | 0.50 | ribosomal | listeriolysin S | Listeria monocytogenes serotype 4b str. F2365
BGC0000569.5 |  | 0.49 | ribosomal | plantazolicin | Bacillus velezensis FZB42
BGC0001173.5 |  | 0.48 | ribosomal | plantazolicin | Bacillus pumilus ATCC 7061
BGC0000605.5 |  | 0.42 | ribosomal | GE37468 | Streptomyces sp. ATCC 55365
BGC0002700.2 |  | 0.41 | ribosomal | bacinapeptin | Bacillus nakamurai
BGC0001887.3 |  | 0.39 | ribosomal | huazacin | Bacillus thuringiensis serovar huazhongensis BGSC 4BD1
BGC0002111.3 |  | 0.39 | ribosomal | andalusicin A, andalusicin B | Bacillus thuringiensis serovar andalousiensis NRRL B-23139

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Similar subclusters
Shows sub-cluster units that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.

No significant matches found.

Transcription Factor Binding Sites
Detailed information for Transcription Factor Binding Site hits, including surrounding genes where applicable.
The strand of the match is shown by an arrow underneath the match.
Detailed documentation is available here.
Binding sites found:
CcpA
Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.98 out of 20.97
Confidence: strong
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.45 out of 26.06
Confidence: strong
CcpA
Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.03 out of 20.97
Confidence: medium
PerR
Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 24.68 out of 28.14
Confidence: medium
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.90 out of 26.06
Confidence: medium
Additional weak hits (4):
ClgR
Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.72 out of 30.22
Confidence: weak
HipB
Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.53 out of 33.73
Confidence: weak
FuR variant 2
Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.15 out of 32.74
Confidence: weak
NrdR
Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.38 out of 27.18
Confidence: weak
(Thi)azol(in)e containing peptide predictions
Lists the possible core peptides for each biosynthetic enzyme. Predicted tail sequences are also shown, if present.
Includes CompaRiPPson results for any available databases, with a detailed explanation here.
No core peptides found.
RREFinder
TFBS Finder
Azole RiPPs
RRE predictions
RREfinder results sidepanel.

azole-containing-RiPP protocluster (1212255...1236022)
ETT64_RS06380
  RRE type: Cytolysin_C_RRE
  Location: 0 - 90
  Score: 134.1

TFBS definitions
Shows descriptions for Transcription Factor Binding Site models

Regulator | Description
--- | ---
CcpA | Catabolite control protein A
MogR | Repressor of flagellar motility genes
PerR | Peroxide regulator

Core peptide predictions
Lists the possible core peptides in the region. Each core peptide lists its possible molecular weights and the scores for cleavage site prediction and RODEO. If relevant, other features, such as macrocycle and amidation, will also be listed.
No core peptides found.
NZ_CP035435.1 - Region 4 - RiPP-like
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,375,898 - 1,387,790 nt. (total: 11,893 nt) Show pHMM detection rules used
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
binding site
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
Tailoring
MIBiG comparison
KnownClusterBlast
SubClusterBlast
TFBS Finder
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Tailoring functions
Tailoring enzymes detected in this region
No tailoring functions detected.
Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | RiPP-like | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000526.5 |  | 0.23 | ribosomal | macedocin | Streptococcus macedonicus

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000526.5 |  | 0.23 | ribosomal | macedocin | Streptococcus macedonicus

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
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No significant matches found.

Similar subclusters
Shows sub-cluster units that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
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No significant matches found.

Transcription Factor Binding Sites
Detailed information for Transcription Factor Binding Site hits, including surrounding genes where applicable.
The strand of the match is shown by an arrow underneath the match.
Detailed documentation is available here.
Binding sites found:
IolR
Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.12 out of 23.79
Confidence: medium
Additional weak hits (2):
RutR
Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.57 out of 26.85
Confidence: weak
OsdR
Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 18.45 out of 30.12
Confidence: weak
TFBS Finder
TFBS definitions
Shows descriptions for Transcription Factor Binding Site models

Regulator | Description
--- | ---
IolR | Regulator of the inositol catabolism

NZ_CP035435.1 - Region 5 - lanthipeptide-class-ii
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,510,740 - 1,533,574 nt. (total: 22,835 nt) Show pHMM detection rules used
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
binding site
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
Tailoring
MIBiG comparison
KnownClusterBlast
SubClusterBlast
Lanthipeptides
TFBS Finder
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Tailoring functions
Tailoring enzymes detected in this region
Transferases: kinase
ETT64_RS07930: PfkB domain protein

smcogs
  SMCOG1276: PfkB domain protein

Lyases: cyclase
ETT64_RS07900: lanthionine synthetase C family protein

smcogs
  SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | lanthipeptide-class-ii | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000548.4 |  | 0.77 | ribosomal | salivaricin A | Streptococcus salivarius
BGC0001787.3 |  | 0.56 | ribosomal | suicin 3908 | Streptococcus suis
BGC0002698.2 |  | 0.51 | ribosomal | phaeornamide | Pseudophaeobacter arcticus DSM 23566
BGC0002005.2 |  | 0.50 | ribosomal | RaxX | Xanthomonas oryzae pv. oryzae
BGC0001210.5 |  | 0.50 | ribosomal | pseudomycoicidin | Bacillus pseudomycoides DSM 12442
BGC0000516.4 |  | 0.50 | ribosomal | geobacillin II | Geobacillus thermodenitrificans
BGC0000500.5 |  | 0.47 | ribosomal | carnolysin A1, carnolysin A2 | Carnobacterium maltaromaticum
BGC0000498.5 |  | 0.47 | ribosomal | bovicin HJ50 | Streptococcus equinus
BGC0000561.5 |  | 0.47 | ribosomal | thermophilin 1277 | Streptococcus thermophilus
BGC0001391.3 |  | 0.45 | ribosomal | thusin | Bacillus thuringiensis serovar rongseni

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000548.4 |  | 0.77 | ribosomal | salivaricin A | Streptococcus salivarius
BGC0001787.3 |  | 0.56 | ribosomal | suicin 3908 | Streptococcus suis
BGC0002698.2 |  | 0.51 | ribosomal | phaeornamide | Pseudophaeobacter arcticus DSM 23566
BGC0002005.2 |  | 0.50 | ribosomal | RaxX | Xanthomonas oryzae pv. oryzae
BGC0001210.5 |  | 0.50 | ribosomal | pseudomycoicidin | Bacillus pseudomycoides DSM 12442
BGC0000516.4 |  | 0.50 | ribosomal | geobacillin II | Geobacillus thermodenitrificans
BGC0000500.5 |  | 0.47 | ribosomal | carnolysin A1, carnolysin A2 | Carnobacterium maltaromaticum
BGC0000498.5 |  | 0.47 | ribosomal | bovicin HJ50 | Streptococcus equinus
BGC0000561.5 |  | 0.47 | ribosomal | thermophilin 1277 | Streptococcus thermophilus
BGC0001391.3 |  | 0.45 | ribosomal | thusin | Bacillus thuringiensis serovar rongseni

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Similar subclusters
Shows sub-cluster units that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.

No significant matches found.

Lanthipeptide predictions
Shows the possible core peptides for each biosynthetic enzyme.
Includes CompaRiPPson results for any available databases, with a detailed explanation here.

#### Lanthipeptide(s) for ETT64_RS07900

ETT64_RS07905
Leader:
MSFMKNSKDILTNAIEEVSEKELMEVAGG
Core:
K K G S G W F A T I T D D C P N S V F V C C
Core with dehydrations:
K K G Dha G W F A Dhb I Dhb D D Cys P N Dha V F V Cys Cys

#### antiSMASH-DB 4.0 matches for ETT64_RS07905

100.0%
NC_004070.1 and 26 others:
NC_008024.1 Lanthipeptides: MGAS10750_RS08460_lanthipeptide NZ_CAAHHZ010000002.1 Lanthipeptides: E9518_RS02835_lanthipeptide NZ_CAAHIH010000002.1 Lanthipeptides: E9602_RS03585_lanthipeptide NZ_CAAHIO010000002.1 Lanthipeptides: E9584_RS04360_lanthipeptide NZ_CAAHJE010000002.1 Lanthipeptides: E9605_RS03435_lanthipeptide NZ_CAAHJS010000002.1 Lanthipeptides: E9628_RS02300_lanthipeptide NZ_CAAHKC010000012.1 Lanthipeptides: E9636_RS08085_lanthipeptide NZ_CAAHMR010000003.1 Lanthipeptides: E9727_RS05510_lanthipeptide NZ_CAAHQE010000003.1 Lanthipeptides: E9931_RS04945_lanthipeptide NZ_CAAHQG010000004.1 Lanthipeptides: E9903_RS05690_lanthipeptide NZ_CAAIFD010000008.1 Lanthipeptides: FAG63_RS07845_lanthipeptide NZ_CAAJDK010000010.1 Lanthipeptides: FAO79_RS08000_lanthipeptide NZ_CAAJFI010000003.1 Lanthipeptides: FAP60_RS03740_lanthipeptide NZ_CP008926.1 Lanthipeptides: DP15_RS03855_lanthipeptide NZ_CP035434.1 Lanthipeptides: ETT65_RS08240_lanthipeptide NZ_CP035435.1 Lanthipeptides: ETT64_RS07905_lanthipeptide NZ_CP035447.1 Lanthipeptides: ETT52_RS07920_lanthipeptide NZ_CP035449.1 Lanthipeptides: ETT50_RS08255_lanthipeptide NZ_CP035453.1 Lanthipeptides: ETT46_RS08220_lanthipeptide NZ_CP060646.1 Lanthipeptides: H7789_RS07920_lanthipeptide NZ_CP065054.1 Lanthipeptides: I2432_RS09105_lanthipeptide NZ_LRGW01000003.1 Lanthipeptides: AWU01_RS03260_lanthipeptide NZ_LS483298.1 Lanthipeptides: DQM39_RS08650_lanthipeptide NZ_LS483407.1 Lanthipeptides: DQM86_RS08100_lanthipeptide NZ_LS483425.1 Lanthipeptides: DQN36_RS07865_lanthipeptide NZ_LS483430.1 Lanthipeptides: DQN28_RS07600_lanthipeptide
Lanthipeptides: SPYM3_RS08605_lanthipeptide
1
K K G S G W F A T I T D D C P N S V F V C C
22
K K G S G W F A T I T D D C P N S V F V C C
1
K K G S G W F A T I T D D C P N S V F V C C
22
95.5%
NZ_CAAIJM010000014.1:
Lanthipeptides: FAI23_RS09465_lanthipeptide
1
K K G S G W F A T I T D D C P N S V F V C C
22
K K S G W F A T I T D D C P N S V F V C C
1
K K V S G W F A T I T D D C P N S V F V C C
22
90.9%
NZ_CAJHJN010000010.1 and 4 others:
NZ_CP066093.1 Lanthipeptides: I6I02_RS06995_lanthipeptide NZ_JACLQK010000029.1 Lanthipeptides: H7U04_RS10750_lanthipeptide NZ_JADPEB010000003.1 Lanthipeptides: I4U26_RS02855_lanthipeptide NZ_KV832031.1 Lanthipeptides: HMPREF2637_RS05170_lanthipeptide
Lanthipeptides: JMZ68_RS08975_lanthipeptide
1
K K G S G W F A T I T D D C P N S V F V C C
22
K+G+G W F A T I T D D C P N S V F V C C
1
K R G T G W F A T I T D D C P N S V F V C C
22
with 66 more sequence matches
Only the first 10 are displayed here.
86.4%
NZ_JAQDVH010000019.1 and 3 others:
NZ_JAQDVH010000019.1 Lanthipeptides: PGU04_RS09335_lanthipeptide NZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06620_lanthipeptide NZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06620_lanthipeptide
Lanthipeptides: PGU04_RS09335_lanthipeptide
1
K K G S G W F A T I T D D C P N S V F V C C
22
K K G G W A T I T D D C P N S+F V C C
1
K K G P G W I A T I T D D C P N S I F V C C
22
86.4%
NZ_PKIA01000004.1:
Lanthipeptides: CYK22_RS03780_lanthipeptide
1
K K G S G W F A T I T D D C P N S V F V C C
22
K+G G W A T I T D D C P N S V F V C C
1
K R G P G W I A T I T D D C P N S V F V C C
22
81.8%
NZ_CP018188.1 and 1 other:
NZ_VDNV01000006.1 Lanthipeptides: FSV44_RS08680_lanthipeptide
Lanthipeptides: BSR19_RS10820_lanthipeptide
1
K K G S G W F A T I T D D C P N S V F V C C
22
K+G G W A T I T D D C P N S+F V C C
1
K R G P G W I A T I T D D C P N S I F V C C
22
77.3%
NZ_CP048273.1 and 3 others:
NZ_CP048273.1 Lanthipeptides: M654_RS18555_lanthipeptide NZ_LT745776.1 Lanthipeptides: B2C81_RS11280_lanthipeptide NZ_LT745776.1 Lanthipeptides: B2C81_RS11275_lanthipeptide
Lanthipeptides: M654_RS18560_lanthipeptide
2
K G S G W F A T I T D D C P N S V F V C C
22
G G W T I T D D C P N S V F V C C
1
A G C G W A C T I T D D C P N S V F V C C
21
77.3%
NZ_CP048890.1 and 3 others:
NZ_CP071478.1 Lanthipeptides: J0P05_RS32905_lanthipeptide NZ_LUCO01000008.1 Lanthipeptides: BMBG_RS24280_lanthipeptide NZ_NVLN01000014.1 Lanthipeptides: CON11_RS24965_lanthipeptide
Lanthipeptides: BMG_RS30865_lanthipeptide
2
K G S G W F A T I T D D C P N S V F V C C
22
G G W T I T D D C P N S V F V C C
1
A G C G W S C T I T D D C P N S V F V C C
21
77.3%
NZ_NIHT01000008.1 and 1 other:
NZ_NIHT01000008.1 Lanthipeptides: CDL23_RS06740_lanthipeptide
Lanthipeptides: CDL23_RS06745_lanthipeptide
2
K G S G W F A T I T D D C P N S V F V C C
22
G S G W T I T D D C P N S+F V C C
2
N G S G W L P T I T D D C P N S I F V C C
22
72.7%
NZ_CP036073.1 and 11 others:
NZ_CP036073.1 Lanthipeptides: EXW45_RS04930_lanthipeptide NZ_CP036073.1 Lanthipeptides: EXW45_RS04925_lanthipeptide NZ_CP036073.1 Lanthipeptides: EXW45_RS04920_lanthipeptide NZ_CP036073.1 Lanthipeptides: EXW45_RS04915_lanthipeptide NZ_CP036073.1 Lanthipeptides: EXW45_RS04910_lanthipeptide NZ_NUEG01000031.1 Lanthipeptides: CN905_RS18915_lanthipeptide NZ_NUEG01000031.1 Lanthipeptides: CN905_RS18910_lanthipeptide NZ_PVRT01000001.1 Lanthipeptides: C6359_RS05275_lanthipeptide NZ_PVRT01000001.1 Lanthipeptides: C6359_RS05270_lanthipeptide NZ_PVRT01000001.1 Lanthipeptides: C6359_RS05265_lanthipeptide NZ_PVRT01000001.1 Lanthipeptides: C6359_RS05260_lanthipeptide
Lanthipeptides: EXW45_RS04935_lanthipeptide
2
K G S G W F A T I T D D C P N S V F V C C
22
G G W T+T D D C P N S V F V C C
1
A G C G W L C T V T D D C P N S V F V C C
21
72.7%
NZ_JANHJQ010000013.1:
Lanthipeptides: NON16_RS14160_lanthipeptide
2
K G S G W F A T I T D D C P N S V F V C C
22
G G W T+T D D C P N S V F V C C
1
D G C G W S C T V T D D C P N S V F V C C
21
68.2%
NZ_MJEH01000022.1 and 1 other:
NZ_MJEH01000022.1 Lanthipeptides: BFG57_RS18360_lanthipeptide
Lanthipeptides: BFG57_RS18365_lanthipeptide
3
G S G W F A T I T D D C P N S V F V C C
22
G G W +T I T D D C P N S+ V C C
1
G D G W I S T I T D D C P N S I I V C C
20
68.2%
NZ_MOWR01000001.1:
Lanthipeptides: BMI76_RS01705_lanthipeptide
2
K G S G W F A T I T D D C P N S V F V C C
22
+ G W T I T D+C P N S V F V C C
1
R W Y G W S F T I T D E C P N S V F V C C
21

#### MIBiG 4.0 matches for ETT64_RS07905

17.4%
BGC0002111.3 (EVG22_32245):
Lanthipeptide: andalusicin A or andalusicin B
5
G W F A T I T D D C
14
W +T I+++C
14
A W A S T I S N N C
23

#### Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine
Transcription Factor Binding Sites
Detailed information for Transcription Factor Binding Site hits, including surrounding genes where applicable.
The strand of the match is shown by an arrow underneath the match.
Detailed documentation is available here.
Binding sites found:
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.03 out of 26.06
Confidence: strong
FuR variant 1
Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.28 out of 29.38
Confidence: medium
MogR
Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.23 out of 26.06
Confidence: medium
ZuR variant 1
Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 23.40 out of 28.19
Confidence: medium
CatR
Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 21.45 out of 25.31
Confidence: medium
Additional weak hits (3):
MntR
Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 26.44 out of 39.62
Confidence: weak
AfsQ1
Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.35 out of 19.52
Confidence: weak
FuR variant 2
Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.24 out of 32.74
Confidence: weak
Lanthipeptides
TFBS Finder
Core peptide predictions
Lists the possible core peptides in the region. Each core peptide lists the number of lanthionine bridges, possible molecular weights, and the scores for cleavage site prediction and RODEO.

ETT64_RS07905 - Class II
  Cleavage pHMM score: 4.40
  RODEO score: 0
  Monoisotopic mass: 2305.1 Da
  Molecular weight: 2306.6 Da
  Number of bridges: 3
  Alternative weights
    (assuming N unmodified Ser/Thr residues)
    2324.6 Da N = 1

TFBS definitions
Shows descriptions for Transcription Factor Binding Site models

Regulator | Description
--- | ---
CatR | H2O2-responsive repressor
FuR variant 1 | Ferric Uptake Regulator
MogR | Repressor of flagellar motility genes
ZuR variant 1 | Zinc-responsive repressor