LOCUS       NZ_CP035431            24230 bp    DNA     circular CON 15-OCT-2024
DEFINITION  Streptococcus pyogenes strain emm105 chromosome, complete genome.
ACCESSION   NZ_CP035431
VERSION     NZ_CP035431.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1800116)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1800116)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005161125.1-RS_2024_10_13
            Annotation Date                   :: 10/13/2024 02:15:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,766
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 39 of 77
            Pseudo Genes (incomplete)         :: 46 of 77
            Pseudo Genes (internal stop)      :: 21 of 77
            Pseudo Genes (multiple problems)  :: 21 of 77
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:05:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 823359
            Orig. end                         :: 847589
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035431.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..24230
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-i"
                     /region_number="4"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..24230
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..24230
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:14230](+)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..14230
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            complement(155..1531)
                     /gene="mnmE"
                     /locus_tag="ETT68_RS04335"
                     /old_locus_tag="ETT68_04330"
     CDS             complement(155..1531)
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="mnmE"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /locus_tag="ETT68_RS04335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04330"
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="WP_136099053.1"
                     /transl_table=11
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNL
                     EQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEILQL
                     LIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLSQLI
                     NDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRGKILR
                     EGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAG
                     IRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDWALLNLSQDSNRIILLNKT
                     DLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLSNVRHISL
                     IEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
                     "
     misc_feature    1702..1837
                     /db_xref="RFAM:RF00557"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
     gene            1866..2366
                     /gene="rplJ"
                     /locus_tag="ETT68_RS04340"
                     /old_locus_tag="ETT68_04335"
     CDS             1866..2366
                     /GO_component="GO:0015934 - large ribosomal subunit
                     [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rplJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /locus_tag="ETT68_RS04340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04335"
                     /product="50S ribosomal protein L10"
                     /protein_id="WP_002984821.1"
                     /transl_table=11
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRRS
                     LRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTKTA
                     DALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAENKE
                     GAA"
     gene            2431..2796
                     /gene="rplL"
                     /locus_tag="ETT68_RS04345"
                     /old_locus_tag="ETT68_04340"
     CDS             2431..2796
                     /GO_component="GO:0005840 - ribosome [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rplL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /locus_tag="ETT68_RS04345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04340"
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="WP_002984819.1"
                     /transl_table=11
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAAG
                     GAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAAEA
                     EEIKAKLEEAGATITLK"
     gene            <3055..3654
                     /locus_tag="ETT68_RS09055"
                     /pseudo=""
     CDS             <3055..3654
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /locus_tag="ETT68_RS09055"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="replication initiator protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="AIYLIYSNSK"
     gene            3829..>4154
                     /gene="dcm"
                     /locus_tag="ETT68_RS04355"
                     /old_locus_tag="ETT68_04350"
                     /pseudo=""
     CDS             3829..>4154
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="dcm"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /locus_tag="ETT68_RS04355"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="ETT68_04350"
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MQFLGLFAGIRGFRLGLINQCHECIGFCEIDKFARQSYKAIYETE
                     GEIEFHDIRQVTDQDFRQLRGQVDIICGGFSLVKHFHSQADDWDLRALGELSSLRLLER
                     PNR"
     gene            <4145..5639
                     /locus_tag="ETT68_RS09250"
                     /pseudo=""
     CDS             <4145..5639
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294852.1"
                     /locus_tag="ETT68_RS09250"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="SAG1250 family conjugative relaxase"
                     /pseudo=""
                     /transl_table=11
                     /translation="TDKNHTHNHIQSTPLTVIQIKN"
     gene            5790..6476
                     /locus_tag="ETT68_RS04375"
                     /old_locus_tag="ETT68_04370"
     CDS             5790..6476
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /locus_tag="ETT68_RS04375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04370"
                     /product="response regulator transcription factor"
                     /protein_id="WP_002984807.1"
                     /transl_table=11
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFKG
                     YDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKPFS
                     LKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYREYE
                     ILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKTIRGI
                     GYKWDV"
     gene            6469..7815
                     /locus_tag="ETT68_RS04380"
                     /old_locus_tag="ETT68_04375"
     CDS             6469..7815
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984805.1"
                     /locus_tag="ETT68_RS04380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04375"
                     /product="sensor histidine kinase KdpD"
                     /protein_id="WP_002984805.1"
                     /transl_table=11
                     /translation="MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTSH
                     NVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKTDKAMEGKYLSKEFSLFNEVAEEKN
                     NLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIVVAL
                     TRFVKEISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIERE
                     ILEKQDLSFQIAALSHDIKTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYF
                     NSLISYTRMLSEDRSVKLILVEELLSELHFEVDDLLNINNIEFSICNRLIITSFYGDEE
                     NLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFY
                     TEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI"
     misc_feature    complement(7844..7859)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.51"
                     /tool="antismash"
     gene            7972..8112
                     /locus_tag="ETT68_RS04385"
                     /old_locus_tag="ETT68_04380"
     CDS             7972..8112
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /locus_tag="ETT68_RS04385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04380"
                     /product="lantibiotic streptin"
                     /protein_id="WP_002984803.1"
                     /sec_met_domain="TIGR03731 (E-value: 7.9e-09, bitscore:
                     32.1, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTTV
                     K"
     gene            8227..10017
                     /locus_tag="ETT68_RS04390"
                     /old_locus_tag="ETT68_04385"
     CDS             8227..10017
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /locus_tag="ETT68_RS04390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04385"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_063631358.1"
                     /transl_table=11
                     /translation="MIKAQLKNLIKTIHLVKEAGFSLLVFSIAISLFQGILPIFSMLLV
                     QKMLNIITTDIKNFHTLMIAFISYIALTLLTIIIGEVDSYINTKLQILLHYKMNHLVMQ
                     KTVKLTLAEFETPEIYDDITRIQNQISYKPFQIYKSIISVLSSLVSLISSFVILLNWKI
                     SIFPLLLILPIVSIYIYLKIGKNEFEILYKRSSDERANWYISHLLTHDFAVKELRLGTL
                     ESYFLKKYDKNNQLFINQENSINRSKMKYGIVLGVLEIAIQSIIMFLAVREAFLGILLV
                     GNVTTFIRSLSTMQTSTQNIVNNIYSFYNGSLYMELLYDFTHDELVHNPIIESTVDTIQ
                     SVEFERVSFSYNGKDNVINDISFSIKAGERLAIVGENGSGKSTIFKLACGLYDNYEGNI
                     YINGINLRSIQKKSYYKRISALFQDFLKYELTLRENVGLGELSKLYSDEDLISALKYTG
                     VDSIFYFNQEKLNQIDLEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEADCYLLDEPSS
                     ALDPESETKIFKTFFELSQNKIGIFITHKPSITQSVNKILYLDNGCIAETGSFADLNIK
                     GMKFKNLLDKEIGATYDTSN"
     gene            10001..11245
                     /locus_tag="ETT68_RS04395"
                     /old_locus_tag="ETT68_04390"
     CDS             10001..11245
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /locus_tag="ETT68_RS04395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04390"
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_002989896.1"
                     /sec_met_domain="LANC_like (E-value: 6.9e-78, bitscore:
                     258.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPQIKQIVDYIADSFINYEQIVAESENKVLINIESKYRNTFFDK
                     SLSHGVPSLILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAGTAGIAL
                     AIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVNGFSGITN
                     YLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLGIAHGITGPMLILAK
                     LKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMAWCYGT
                     PGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIYDLIGKN
                     YSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVSGIVLFLL
                     NPYSDEELLWQRIFI"
     gene            11264..14230
                     /locus_tag="ETT68_RS04400"
                     /old_locus_tag="ETT68_04395"
     CDS             11264..14230
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /locus_tag="ETT68_RS04400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04395"
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_011284760.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.9e-90, bitscore:
                     299.9, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 1.5e-11,
                     bitscore: 41.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.7e-13, bitscore:
                     47.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSLY
                     DSVIDFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKVSF
                     IKKAVPDYSWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTVNESEEDRNISFS
                     KPIKLLVEKCKTRYIKYEELIDVFKNNYPDIVSDMLESYIHDLVTNDFLISDLRPPICN
                     INSLDYLLSKLEEGTLCTDLTTLKKMIEDYNDLNIGEGRDLLLQIFDKMESIYKYDNGG
                     NYLKVDSLAEYEGGFNILSETARESIERLAEMFVKLNTNVKRRGGRKNSLEEYQLKFIG
                     KYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQMFEKKYEEALLNGGHIE
                     FYSKDLDDFQTDQSNSLDSFELNFNIKIINNDVKLYLGANIGSGQAGRSFGRFYGLSET
                     VRETIKNLNHQNNTNVELSFVPKQIRLANVIQNYSDESYNTSFFTTSWDSENELRLEDI
                     YIRYSDGKFHFTTIDGKNELKFTMNNMLNSDSQSRVLRLLVDLSEFEYGLSHWSLFPWD
                     ILAQERVYIPEILFEDITITTAQWNLSAIREEIIHQKIFTGKKDIFNKFRKTYKIPKDI
                     ILKYADNELRLDLSIDSDLEILFKNINKYRFVSLRGIEQGESIFKDNLGNYNTEIVVPI
                     LKESNGINNTNDNVQFKNEVKKIYLPFCDWLFFKFYGPKERQEELLNNWKKFWLNLPSE
                     LFSQSNMFYMRYNDTNDHIRIRINCDSIENNFSLYLSVVQDLLPQLIESCIISDIEVSS
                     YKPEVNRYGGPHLISYAEEIFCKESILFLNNTIEFSDDERLVCATYLVLYYLNHFFKDK
                     ETRRSFLFENYTGKYKKDFKNLPIDLPIEYMKILNGVTSALDRYDFFQEMDRYLMSYMK
                     EYNRFGGTNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKVYCFAYYTLNAQQYIEWE"
     aSDomain        11666..11920
                     /aSDomain="Lanthipeptide_LanB_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing LanB protein in a class I
                     lanthipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_ETT68_RS04400_Lanthipeptide_LanB_RRE.
                     1"
                     /evalue="2.60E-13"
                     /identifier="RREFam001.1"
                     /label="ETT68_RS04400"
                     /locus_tag="ETT68_RS04400"
                     /protein_end="219"
                     /protein_start="134"
                     /score="41.5"
                     /tool="antismash"
                     /translation="NTAAFTQGDRYVLPFTVNESEEDRNISFSKPIKLLVEKCKTRYIK
                     YEELIDVFKNNYPDIVSDMLESYIHDLVTNDFLISDLRPP"
     gene            14236..14925
                     /locus_tag="ETT68_RS04405"
                     /old_locus_tag="ETT68_04400"
     CDS             14236..14925
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /locus_tag="ETT68_RS04405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04400"
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="WP_021775230.1"
                     /transl_table=11
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIMK
                     ILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVSES
                     TILPLLNKVGLGDIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIGIQE
                     LREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIVLEKEYDGSENLEELFNNQ
                     ILFEKRR"
     gene            14931..15680
                     /locus_tag="ETT68_RS04410"
                     /old_locus_tag="ETT68_04405"
     CDS             14931..15680
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /locus_tag="ETT68_RS04410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04405"
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="WP_002989889.1"
                     /transl_table=11
                     /translation="MVQIFLSNLFKIKNTSISKLILIFPIICVMLSLLFSALGGESILR
                     LAAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLIAL
                     INLIISMILSLMLIVISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKINVY
                     FAMIINIISPLIIGTSFSLLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPDYSQI
                     FISLFLGIAFFILLTNLFAISFKKQVK"
     gene            15683..16405
                     /locus_tag="ETT68_RS04415"
                     /old_locus_tag="ETT68_04410"
     CDS             15683..16405
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /locus_tag="ETT68_RS04415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04410"
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="WP_002989886.1"
                     /transl_table=11
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQSR
                     TVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFLWLC
                     YCIVLIISITIFILLLKTIGINVSLRLLILNSLIYIIFAYVTYLINHIISYIFSTGVAL
                     GISMVGVIVAAFCETSLGDKVWFLIPWAWLLRISDTLYNQQKMAIVPLIVIFFVSCTVA
                     LLHIRIFKSWNQDCLTSS"
     gene            16596..16814
                     /locus_tag="ETT68_RS04420"
                     /old_locus_tag="ETT68_04415"
     CDS             16596..16814
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /locus_tag="ETT68_RS04420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04415"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002989884.1"
                     /transl_table=11
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADVL
                     VQFSKLYNVSIDYLLGQTDYPYRFHNK"
     misc_feature    complement(16626..16637)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.71"
                     /tool="antismash"
     gene            complement(16885..17028)
                     /locus_tag="ETT68_RS09065"
     CDS             complement(16885..17028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042734.1"
                     /locus_tag="ETT68_RS09065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_021299391.1"
                     /transl_table=11
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHGV
                     SH"
     misc_feature    complement(17086..17101)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     gene            17087..17335
                     /locus_tag="ETT68_RS04430"
                     /old_locus_tag="ETT68_04425"
                     /pseudo=""
     CDS             17087..17335
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106749.1"
                     /locus_tag="ETT68_RS04430"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04425"
                     /product="DUF3173 family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKIVTYKKLDEYWFSRTYCKKYY"
     gene            17338..18525
                     /locus_tag="ETT68_RS09255"
                     /old_locus_tag="ETT68_04430"
                     /pseudo=""
     CDS             17338..18525
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943304.1"
                     /locus_tag="ETT68_RS09255"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04430"
                     /product="tyrosine-type recombinase/integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTIKNQKKYKGVYCDKNGKIFYQADLGVDPVTGKRVQKKARKN"
     gene            18683..19918
                     /gene="pbp3"
                     /locus_tag="ETT68_RS04440"
                     /old_locus_tag="ETT68_04435"
     CDS             18683..19918
                     /EC_number="3.4.16.4"
                     /codon_start=1
                     /gene="pbp3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /locus_tag="ETT68_RS04440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04435"
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="WP_136275535.1"
                     /transl_table=11
                     /translation="MIKRWVVLFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVL
                     YEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFD
                     KKTYSVQDLLTATLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSGKILNASG
                     LPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGNSIKS
                     FNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKNQNTRF
                     EQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVKELPVSVTEDVTIILQQGEQV
                     PKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKASDL
                     QLWWRDFLEKRR"
     gene            complement(20035..20997)
                     /locus_tag="ETT68_RS04445"
                     /old_locus_tag="ETT68_04440"
     CDS             complement(20035..20997)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /locus_tag="ETT68_RS04445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04440"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_002995687.1"
                     /transl_table=11
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGLS
                     DVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPILMY
                     HAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKKVVW
                     LTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGMSFQD
                     HTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAARLNYK
                     LGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            21328..22605
                     /locus_tag="ETT68_RS04450"
                     /old_locus_tag="ETT68_04445"
     CDS             21328..22605
                     /EC_number="6.3.2.-"
                     /GO_function="GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /locus_tag="ETT68_RS04450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04445"
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="WP_136275533.1"
                     /transl_table=11
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIV
                     GTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRIRP
                     LTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAMVVV
                     CPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGASIWE
                     WQEQFQMAENASDYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTPD
                     HIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILFGAIDTK
                     PIVDMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAKADDFFVI
                     TGSLYFISEIRRYWKKHIEKSVLLTH"
     gene            22658..23224
                     /gene="folE"
                     /locus_tag="ETT68_RS04455"
                     /old_locus_tag="ETT68_04450"
     CDS             22658..23224
                     /EC_number="3.5.4.16"
                     /GO_function="GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA]"
                     /GO_process="GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="folE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GTP_cyclohydroI"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989871.1"
                     /locus_tag="ETT68_RS04455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04450"
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="WP_032460184.1"
                     /sec_met_domain="GTP_cyclohydroI (E-value: 9.3e-77,
                     bitscore: 253.1, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKMYAEMFLG
                     LGKDPKEEFTAVFKEHHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSDGRVTGLSK
                     LARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRGIKKPGSKTI
                     TTTARGLYKESRAERQEVISLMTKD"
     gene            23233..24033
                     /gene="folP"
                     /locus_tag="ETT68_RS04460"
                     /old_locus_tag="ETT68_04455"
     CDS             23233..24033
                     /EC_number="2.5.1.15"
                     /GO_function="GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="folP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /locus_tag="ETT68_RS04460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_04455"
                     /product="dihydropteroate synthase"
                     /protein_id="WP_136275531.1"
                     /transl_table=11
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQMI
                     ADGAKIIDVGGESTRPGCQFVSAADEIDRVVPVIKAIKENYDILISIDTYKTETARAAL
                     EAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRAQAA
                     LDAGVPKNNIWIDPGFGFAKSVQQNMELLKGLDRVCQLGYPVLFGISRKRVVDALLGGN
                     TKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
ORIGIN
        1 caccaagcta atgaatatat gcctttagaa aatatttatc gctcagcagc tatttatgct
       61 gaagcaatct acgaattaat taaataagat gcttatgata gcaaaaagag ctggaacagt
      121 gttccagctc ttttgtgatg attagagact gttgttattt accaagacag aattgactaa
      181 agagttgggt aatgagttca tcaggagcag catcccctgt gatttctccg agaatttccc
      241 aagttctggt taaatcaacc tgtagtaaat caactggcat gccaagcgcc aagccgtcat
      301 tgacagcttc taaactctgg actgcctttt caatcaaaga aatgtggcga acatttgaca
      361 ggtaagtggc atcttgttct actagtccag cattatcaaa gaagagctgg ttgatacgat
      421 cttcgattag attaatgttc tggttggtta aaacggaaat gggaatgtag tcatctggta
      481 attgctctaa ttcaatcttt tgttccaaat ccgttttatt caacaatata atacggttac
      541 tgtcctgact gagatttaac aaagcccagt cttggtcggt cagtttttca gaagcattca
      601 gcacaagaag aacaaggtca gcttcttgaa gagctttttt agaccgttca actccgattt
      661 gttcgaccag atcatctgtc tcacgaatac cggccgtatc gacaagtttt agaggaactc
      721 ccttgatatt aacgtattct tcaatgacat ctcgggtggt tcctgcaata tcagtcacaa
      781 tagccttgtc ttctcgcaac aaattattaa gtaagcttga cttaccgacg tttggacgac
      841 caataatagc tgttgataag ccctcacgca gaattttacc gcgtttagct gttcgaagga
      901 gattttccaa aaggctttgg aattcttgtg ttttctcacg tagcagagca gtggtcattt
      961 cttccacatc gtcgtattca gggtagtcaa tattgacttc gacttgagct aaggtgttga
     1021 gaatttcctg acgggtgtca ttgataagct gggaaaggga gccatccagt tgcttgactg
     1081 caatcgtcat agccttgtct gttttggcac gaataatgtc catgacagcc tcagcttgag
     1141 tcaaatcaac tcgcccattt aagaaggcac gtttggtgaa ttcgccaggc tctgccattc
     1201 tagcgccttg gcgaatcaat agttgcaaga tttcatttgt cacagcaata ccaccgtgcg
     1261 tattgatttc aaccacattt tcacgggtaa aggttttggg ggctagcatg actgacacca
     1321 taacttcatc gataatagtt cctgtcttag gatcaatgat atggccatag ttaatggtgt
     1381 gtgaggcgac ttgctctaag ttctttccct taaaaacaga ttgtgcaatg gcaagcgcat
     1441 ctgtcccgga tagacgaaca atgccaatag ctccttcacc aagaggggtt gaaatggcgg
     1501 taattgtatc aaattcttta gttatactca tgccctctat tttaaatcaa gtcctagtag
     1561 aaagcaaggt attattttgt atagaaaata ggactagcac cattttgagt ttagctaata
     1621 ctaaaaaaaa taaatgtaaa aaaatcattt ttttaataat ttttattgac aatagtatta
     1681 taaaagaata taatgatata ggttttgata aaaaactgac ctaagacagc aggggagcat
     1741 gctcataata ttcctgccga ggcacaaaac gtgattaaag aaaataacgt atttgtactt
     1801 tcgtgtctag gtttaggtgc gttttttttg tacctagccc aaaaataaaa acggaggtaa
     1861 aactaatgag tgaagcaatt attgctaaaa aagctgaaca agtcgaactt attgctgaga
     1921 aaatgaaagc agcagcaagt atcgttgttg ttgattcacg tggtcttaca gttgatcaag
     1981 atactgttct tcgtcgttca cttcgtgaaa gtggcgttga gttcaaagtt atcaaaaact
     2041 caattttgac tcgtgcagct gaaaaagcag gtcttgatga gttgaaagat gtattcgtag
     2101 gaccatctgc agtagcattt tctaacgaag atgttatcgc accagctaaa gttatcaacg
     2161 actttactaa aactgctgac gcacttgaaa tcaaaggtgg tgcaatcgaa ggcgctgttt
     2221 cttcaaaaga agaaatccaa gcacttgcaa cattgccaaa ccgcgaagga atgctttcta
     2281 tgcttctttc tgtacttcaa gctccagtcc gcaacgttgc atacgctgtc aaagctgttg
     2341 cagaaaacaa agaaggcgct gcttaagacg tctcagagta gcttaaggct acgcttaaca
     2401 ttttaaatta attatttgga ggaaatcaca atggcattga acattgaaaa cattattgct
     2461 gaaattaaag aagcttcaat ccttgagctt aacgatcttg taaaagctat cgaagaagaa
     2521 tttggtgtaa ctgcagctgc tcctgtagct gctgcagctg ctggtggtgc tgaagaagct
     2581 gctaaagatt cattcgacgt tgaattgaca tctgctggtg acaaaaaagt tggcgttatc
     2641 aaagctgttc gtgaaatcac aggtcttggt cttaaagaag ctaaaggtct tgttgatgga
     2701 gcacctgcta acgttaaaga aggcgttgct gctgcagaag ctgaagaaat caaagctaaa
     2761 cttgaagaag ctggagcaac aatcactctt aaataagaag cagatagcaa tttgagagcg
     2821 aaatcccgat ttatcagtct tttagagctt atagcgattt gaagaaactg ataaaatgat
     2881 ttggtttttt gccaaaaatc ctgccaaaaa aatctttggc aggattttta ttttctataa
     2941 aaattaagct gagtagagtg gttattctat ctatatgtgt ctagtaataa tttttttatt
     3001 ttcaggagga aaatcatgca aatgaatgaa cagtcatcac gacaaatggt gattgccatc
     3061 tatctcattt attccaactc aaaatgatag tactccttgg ctgttccaag tcgaaattgt
     3121 tagtgattaa gaaaacctac gagactatgg tttgatcgat aaaggccagc agtctcccag
     3181 tgaaaaaggg cgtttggcta ataagattta tttaggaaag ttggaacatg atttcacccc
     3241 agtcttaagt tcagacgggg ccagtctcaa aaacagacca gccccgtgtt aaattcagcc
     3301 cctagtgaga ctgaaaagag tgaaactgaa ggtagtgaaa ctaaagagag taatttagtc
     3361 attgaggacg aggaggaaag aaaggagtgc actagtgtga aaaagactga gggtcacttt
     3421 actcgtcaag ttgatcatgt caccaaatat gacaaagatt atatttggag tttggttcat
     3481 tcccaattac gtgaaggtgg tttatcacaa gctgctagtg acttagtcat gagctatttt
     3541 gaagaacgct atgcttatgc gctggaacac atacggttcg caagaacagc tgaagctatt
     3601 gcggaatatg tctttaatgg tgtcttatcc gaatggacca agcaactaag gtgacaagaa
     3661 gcgaagtgag taagatgttt tggtggctat tattaggtgt ttggggacta aggacaattt
     3721 ggttgattat tgaagttatc actgcaccag aaatggaaga ttatagttga gtaaggacgt
     3781 tacataagca gtaaggtcct tttattttgt cagaaaggaa tgagagatat gcagttttta
     3841 ggtttgtttg caggaattag aggctttcga ttgggtttaa ttaatcagtg ccatgagtgt
     3901 attggctttt gtgagattga taagtttgcg aggcaatctt acaaagccat ctatgagacc
     3961 gaaggagaaa tagaatttca tgacattaga caagtcacag accaagactt tagacaactt
     4021 agagggcaag tggatatcat ctgcggtggt ttttcccttg tcaagcattt tcactcgcag
     4081 gcagacgact gggatttgag ggcactagga gaactctctt ctttgagatt actcgagcga
     4141 ccaaacagat aaaaatcata cccataatca catccaatca acgccattga ccgtaattca
     4201 gataaaaaat tgatatggaa ttatgccttg gaacgaaatc ttcgtatgat ctcagaccgt
     4261 atttctaaaa tagctggggc gaaaattatt gaaaagcatt tctcttaccg tgactatcaa
     4321 aaatataaag cgactagcca taagtttgaa ctaaagcaac gtctttattt tttgatgcag
     4381 cagtcaaagt tctttgatga ttttttagaa aaagcagagt aattacatgt tcatattgat
     4441 tttagtcaga agcatagcag attcatgatg acggatcgag taatgaaaaa accaattcga
     4501 ggccgtcaac tcagtaaacg agatttatat gatgaagaat tttttcgtac acattttgcc
     4561 aagatagaaa ttgaaagtcg attagaattt ttgttgaacc gtgccaattc attagaggag
     4621 ctactattaa aagcaaaaga agtgagtcta accattgact taaaacaaaa aatgtaactt
     4681 ttatcctaga agaagatact caaaagataa gtttaggtca taaaaaaata agcgataaga
     4741 aattatatga tgtcaatttt ttcaagatta ctttaaaaat aaggaagtcg gtgtttcaga
     4801 tcgattagaa agtctacaga aacagtacat gcttttcaag aagaacgaga taaagaggag
     4861 gtatccactg aagagagtaa ggaagtcttc aagaaataca aggggcaacg agatgccatc
     4921 catgagtttg aagttgaact tgtggaacat caaattgaga ggttaattgc tgaggacatt
     4981 tatatcaagg tgtcttttgg tgtaaagcag agtggtctta ttttcattcc taactatcaa
     5041 ttggatatta ggaaagaaga aaatcataaa aaatataaag tctatattcg tgagaccgca
     5101 caattcttca tttacaacaa ggaggcgtct aaacttaatc gctatatgcg agggcgtgag
     5161 ctcatttacc agttaaccaa tgatagcaag agtattccaa aaagaagacg accaaaacca
     5221 ttgatacact taagaaaaag attgaagaaa taagtctcct cattgagtta gatacggaga
     5281 ataagcctta tcaagatatt aaagatgaca ttgttaaaga tatggctcaa ttagatttga
     5341 ccattacaga gttgcaggac catattgctc atctcaataa ggttgcggag gtcttgctta
     5401 atatgaacaa caacgatata gagaaccgcc gtttggccag atatgactat gccaagatga
     5461 acttgactgc agctataaaa atagaagaag ttgagaaaga gattgaaact tctcaaaatg
     5521 aacttaatat atccatagat gagtatgaat atctagtaag aaggttggaa aagtttggag
     5581 agatcttgag ttatagcaaa attatcgata cttctcgaaa tgaaatacaa tgggagtaat
     5641 tatgatataa tttgggtggt agcaattttt atatcagata ttaaaatcta gttgatgttt
     5701 cgtaaataac tatattaaag aaatgattcc tgccgcttac attgagaata gtttatttgt
     5761 aagtatttca ttatttaaaa ggggtaataa tgtataagat tttagccata gatgatgata
     5821 aagaaattct gaagcttatg aagactgctt tggagattga aaattaccat gttataacct
     5881 gtcaagagat agaattacca atagtttttg atgattttaa aggatatgat ttgatcttat
     5941 tggatatcat gatgcctaat ataagtggaa ctgagttttg ttataaaatt cgggaagaag
     6001 ttcattctcc aattattttt gttagtgctt tagatggcga taatgaaatt gtccaagctt
     6061 taaatatagg gggagacgat tttattgtga aaccatttag cttaaaacaa ttcgtagcta
     6121 aagttaactc tcatttgaag agagaagaga gagcaaagat aaagaatgag gctgaggaga
     6181 gagtgaagcg cagttttcca cctatagaaa tctatctaga ggaacgtatg ttatatattg
     6241 ataaacagcc gttattcctg acttatagag agtacgaaat tttagaatta ctgtcacgtc
     6301 atccgtataa agttttcaca aaagaagaga tatatgaaca agtatatagc gatgaagctt
     6361 cagcattgtt tcattctatt tcagaatata tctatcagat tagaatgaaa ttttcaagtt
     6421 ttggaattaa tccaataaaa actattcgcg ggattggtta taaatgggat gtttaaaaaa
     6481 tattcaataa aaaatcgtgt ttggcgcgcc gtcgttgaaa ttgtctttgg agtttgtatc
     6541 actgttttaa tgattgcgat aatatcgctt tctttttcaa agttaaatat agtgacatct
     6601 cataatgtgg gagaagaatt ttatattaaa gataaacagt caatcaaaca gttgaacaat
     6661 tatatgaaga cattgggatt agattatgtt gtttttgata gaaaaacaga taaagctatg
     6721 gaaggaaaat atttatctaa agaattttct ttatttaacg aagttgcaga agaaaaaaat
     6781 aatctgactt ttaattctgt acattatgat ttatatacta atatcaatta taatattgtt
     6841 ataagatata atgagatacc ggagttttct aaccattacc ttagaaatgt ttcatataac
     6901 atgttgacat tttatattct gggaatagga acgagtatca gcattgtagt tgctttaaca
     6961 cggtttgtaa aagaaatttc tttgaatttc aaggaaatca agaagttagc aaataaaatg
     7021 gggatagaag tcctttctga aaatgaaaat tattctaaaa taatcgagtt tgatgatata
     7081 cttagaactt tacatattaa aggtgataat ttgaaaagtt tgattgagcg agaaatattg
     7141 gaaaagcaag atctatcctt tcaaattgca gcactttcgc atgatataaa aacaccttta
     7201 acggttttga aaggaaatct tgagttatta gaattgacta ctttaaataa aaatcaggaa
     7261 ggttatattg tatctatgaa caatagtatt tctgtttttg aaggctattt caattcttta
     7321 atatcttata cacgcatgct ttcagaggat agatcagtga aactaatttt agttgaggag
     7381 ctattaagtg agttacattt tgaagtcgat gacttattga atatcaataa tattgaattt
     7441 tctatttgta atagacttat tatcacttct ttttatggag atgaggaaaa tctgataaga
     7501 gctctttcta accttttagt taatgcaata agatttatgc ccgtattaga taaaaaaatc
     7561 gaagttatac tgtctgagtc aggagagcaa attcattttg aaatatggaa taatggtgag
     7621 cgtttcagtg atagtacact gaaaaaggga gataaattat tttacacgga agattatagc
     7681 agaggtaaca aacattatgg aatcggtttg gcatttgtta aaggcgttgc tatcaaacac
     7741 ggtggaaatc ttcaattgaa taatccagct agaggaggtg ctagcgccat aatctcgata
     7801 aaaaagaaaa tttaatagat ttgattgaaa ctggagtatt tcaggcaaaa aatcgtaaat
     7861 ttttgtgatt tttggctaaa atactttttt tctgaacaaa ttctgaactt ggggtgtcat
     7921 aataatcatg taaatatatc ggatgcaaaa acgtaagaga ggaactagaa aatgaataac
     7981 acaattaaag actttgatct cgatttgaaa acaaataaaa aagacactgc tacaccttat
     8041 gttggtagcc gttacctatg tacccctggt tcttgttgga aattagtttg ctttacaaca
     8101 actgttaaat aataaagtta tcattatcat caacattata atttcaaata atttgatata
     8161 tttataaatt taggggagta gactgatttt agtttgctcc tttattagta gataaggaag
     8221 ttatacatga ttaaagctca gctaaagaat ctaataaaaa caattcatct cgtaaaagag
     8281 gctggtttct ccctactggt gttctcaatt gctattagct tatttcaagg gattttaccc
     8341 atttttagta tgctactagt tcaaaaaatg ttaaacatta taacgactga tataaaaaat
     8401 tttcacacat tgatgattgc ttttatctca tatattgctt taacattatt gacaattata
     8461 ataggagaag ttgatagtta tattaatacc aaattacaga tacttcttca ctataagatg
     8521 aaccatttag ttatgcagaa aactgtaaag ttaacattgg ctgaatttga gacaccagaa
     8581 atctatgatg atatcactcg catacaaaat caaatatctt ataaaccttt tcaaatatat
     8641 aagtcaatta tttctgtatt atcttcgtta gtatcattaa tttcctcatt tgttatttta
     8701 ttaaattgga agatatcaat ttttccactt ttacttatcc ttcctatcgt ttctatatat
     8761 atctacttga aaattggtaa aaatgaattt gagatactat ataaaaggag tagcgatgag
     8821 agagcaaatt ggtatattag tcatctttta acacatgatt ttgctgtcaa ggagttgagg
     8881 ttaggtacgt tagaaagtta ttttctaaaa aaatatgata aaaataatca attatttatt
     8941 aatcaggaaa atagtataaa tagatctaaa atgaaatacg gcatagtatt aggtgtattg
     9001 gagattgcta tacaatctat tattatgttc cttgcagtta gagaagcatt tctaggaata
     9061 ttattagttg gaaatgtaac tacttttata aggtctttat ctacaatgca aacaagtaca
     9121 caaaatatag taaataatat ctattctttc tataatggtt cattgtacat ggagttattg
     9181 tatgatttta cgcatgatga gttagtacat aaccctataa tcgaaagtac ggttgataca
     9241 attcagtcag ttgaatttga aagagtttct ttttcatata atggtaagga taatgtaata
     9301 aacgatataa gtttttcaat taaagcagga gagaggttag caattgttgg tgaaaatggt
     9361 tctggaaaaa gtacgatttt caaattagcc tgcggtcttt acgataatta tgaaggcaat
     9421 atatatataa atggtattaa tcttcgttca atacagaaaa agtcatacta caagagaata
     9481 tccgcattgt tccaagattt tttaaagtat gaattgacac tgcgtgaaaa tgttggattg
     9541 ggtgaattat caaagttata tagtgatgag gatttaataa gtgcattgaa gtatacagga
     9601 gttgattcga tattttactt taatcaggaa aaattaaatc agatagatct ggaacagcaa
     9661 ttagggaatt ggtttgagga tgggagacag ttatctggtg gacagtggca aaaaattgca
     9721 ttatcaagag tatatctcaa agaagcggat tgctatttgc tagatgaacc tagttctgcg
     9781 ttagatcctg agtctgaaac aaaaattttt aaaacatttt ttgaattatc gcaaaataaa
     9841 attggtattt ttattacgca taagccaagc attactcaat ctgtgaataa aattttgtat
     9901 ctagataatg gatgtatagc tgaaacagga agtttcgctg atttaaatat taaaggaatg
     9961 aagtttaaaa acttattaga taaagaaatt ggagccacat atgacacctc aaattaaaca
    10021 gatagtagat tacattgcag atagttttat aaattatgaa cagatagtag ctgaaagtga
    10081 gaataaagta ctaattaata ttgaatctaa atatagaaat accttttttg ataaatctct
    10141 gagtcatggt gtgccatcat taatcttaat gtatagttca ctatataaag tgacaaaaga
    10201 tgagagatat ctaacattct ccaacatata cgttgagaaa ttagtagaca taatctctag
    10261 agatggtata gaatctccgt ctctctatgc tggcactgca ggtattgcat tagctattaa
    10321 agaagcgagt atatctggaa agtattatcc gaaattatta tcatccttaa attgtcttct
    10381 aaaagaacaa attaaagaca aattggtagt aagtttttct aatattgaga aaggaattat
    10441 tgagccatat gattatgata tggttaacgg atttagtgga ataactaatt acttactcct
    10501 tgaacaagaa tttttttctg tagagttaaa tcaaattgga aattatcttt tgaaatatat
    10561 tgagacaatt ttaaataagg taactaattg ttctgaagat agagaagctg aatttgatct
    10621 aggaattgca catggaatta ctggtcctat gctaatccta gcaaaattaa aatcagaaaa
    10681 aattttaagt gtagaagtag gagatatact gaataaagct ataaatttaa tgtttttatt
    10741 tagaagggat gacaaactat ggcctggaaa aatttatact agtaatattt tggatcttga
    10801 tttaaattat cttcctacta gaatggcatg gtgttacggt acgcctggaa ctgcagttgc
    10861 cctttttaaa atgtgtcaat tgatagattt aaattttatt gacgatatta atgaaagttt
    10921 aattgctcag atgagaagtt cagacaaaaa tgtctattct cttacaatgt gccatggttt
    10981 ggcaggagta acttttatat atgacttgat tggtaaaaat tattcaagtg agaggctaac
    11041 tcgctttgca gaagagcttc gccaacaaat aattacatcg ttttcaacta caaaagtatt
    11101 tgggtatact gacagagaaa aatttggaga aatagaagtt gaactggagt caatagggat
    11161 attgcaaggg gtatcaggga tagtgttatt cttattaaat ccttacagtg acgaggagct
    11221 attgtggcaa agaatattta tttaatttga aaaggagaac aaaatgtata aggtatttcc
    11281 aactggtatg attcgtattc cattgcaaaa acaagaattt tataataaag actatgaaga
    11341 tttaataaat gataaagttt ttatggaaca acttttagtt gctagtccca gtctttatga
    11401 tagtgtaata gattttgata aatgtaatga aaagaaaaag caaaatgtaa aagtctcatt
    11461 aaatagttat tctaaaagag cagcgtttag aactactcca tttggtatct ttactgctat
    11521 taatacagtt gatttgacaa agggaaccac ttctaatgta caaaaggtta gttttattaa
    11581 aaaagcagtc ccagattatt catggatata ttctctagta aaatcgtatg aaataagtaa
    11641 ccttgaaaaa ctttcattaa aaataaatac agcggcgttt actcaaggtg acagatatgt
    11701 actaccattt acagtgaatg aatcagagga agatagaaac ataagttttt caaaaccaat
    11761 taagttactt gtagaaaaat gtaaaactcg atatataaaa tatgaagagt taatagatgt
    11821 atttaaaaat aattatccag atatagtgtc agacatgcta gagtcatata tacacgactt
    11881 agttacaaat gattttttaa tttcagattt aagaccacca atttgtaata tcaattcctt
    11941 agattatcta ctatctaaat tagaagaagg aacactatgt actgacttaa ctactttaaa
    12001 gaagatgatt gaagattaca acgatttaaa tattggggaa ggtagagact tacttttgca
    12061 gatttttgat aaaatggaaa gtatttataa atatgataac ggtggaaatt acttaaaagt
    12121 tgattcatta gcagagtatg aaggcggttt taatattcta tctgaaacgg ctcgggaaag
    12181 tatagaacga ctcgcagaga tgtttgtaaa attaaataca aatgtaaaga gaagaggggg
    12241 gaggaaaaat tcacttgaag agtatcagtt gaaatttatt ggcaaatatg gagaaaactg
    12301 ctcaattcct tttgttgaag taataaataa agatgcaggc ataggttttc ctgaatatta
    12361 caaaggagga gaaggtgaag caatagagtt aagcgatcca ataatgcaaa tgtttgaaaa
    12421 gaaatatgag gaagctctat taaatggagg gcatatcgaa ttttattcaa aagatttaga
    12481 tgattttcaa acagatcaaa gcaatagttt agattcgttt gaattaaatt ttaatataaa
    12541 aataataaat aatgatgtga agttatattt aggagctaat atcggttctg gacaagcagg
    12601 tagaagcttt ggaagattct atggattaag cgaaacagta agagaaacaa ttaagaattt
    12661 aaaccatcaa aataatacga atgtagagtt gtcatttgtt cctaaacaga taagacttgc
    12721 gaatgtaatt caaaattata gtgatgagtc ttataatact tcatttttta ccacatcatg
    12781 ggattcagag aacgaactgc gattggagga tatttatata agatatagtg atggtaagtt
    12841 tcattttaca actatcgatg ggaaaaatga gttgaaattt accatgaata atatgctaaa
    12901 ttccgatagc caaagcaggg ttttgagatt gttggttgat ttatctgagt ttgagtacgg
    12961 tttatcgcat tggtcattat ttccttggga tattttggct caggaaagag tatatattcc
    13021 tgaaatttta tttgaagaca ttacgattac cactgctcag tggaatttat cagcaattag
    13081 agaagagatt atacatcaaa aaatatttac tggtaaaaaa gatatattca ataagtttag
    13141 aaagacttat aagataccta aagatattat tttgaaatat gcagataatg aattgaggtt
    13201 agatttatct atagatagtg atttagagat tttatttaaa aacattaata aatataggtt
    13261 tgtttctttg agaggaattg agcagggaga gtctattttt aaggataatt tagggaacta
    13321 caatacagag atagtagttc caattcttaa ggaatctaat ggaataaaca atacaaacga
    13381 taatgttcaa tttaaaaatg aagttaagaa aatatattta ccattttgtg actggctatt
    13441 tttcaaattt tatggtccaa aagagcgaca agaagaatta ctaaataact ggaaaaaatt
    13501 ttggctaaac ctgccatcag aactgtttag ccaaagtaac atgttttata tgagatataa
    13561 tgataccaat gatcatataa gaattcggat aaattgtgat tcgattgaaa ataacttttc
    13621 tttgtatttg tcagtagttc aagatctatt acctcaactg attgagagtt gtattatctc
    13681 tgacatagaa gtgagtagct ataaaccaga agtaaataga tatggaggac ctcatttaat
    13741 aagttatgca gaggaaattt tttgtaaaga atctatctta tttcttaata atacaataga
    13801 attttcagat gatgaaagat tagtttgtgc tacttacttg gttctatatt atctaaatca
    13861 ctttttcaaa gataaagaaa caaggcgctc tttcttattt gagaattata ccggaaagta
    13921 taagaaagat ttcaaaaatt tgccaattga tttacctatt gagtatatga agattttgaa
    13981 tggagtcacc tcagctcttg atagatatga ttttttccaa gaaatggata gatatctcat
    14041 gtcttatatg aaagagtata atcgatttgg tggtacaaat gatatctata atactaaatt
    14101 caatttagta ggaagttttt tacatctatc aatgaatcga ttaaacggga ttgatagaga
    14161 gtttgaagag aaagtgtatt gttttgctta ctatacacta aacgctcagc aatatataga
    14221 atgggagtaa ctaatatgtt gaaaatacaa aatctaaaaa aatcttatgg taagagaacc
    14281 atattgaata atgtcaatat gaatattcct aagggaaaag tgtatgcttt aatcggtcca
    14341 aatggtgctg gaaaatcaac tattatgaaa attttgacag gcttagttag taagacaagt
    14401 ggttctatta tttttgaagg tagagaatgg tcacgtcggg atctgcgaaa aatcgggagt
    14461 attattgaag aaccaccact ttataaaaat ttgagtgctt acgataatat gaaggtagtt
    14521 acaacaatgc ttggtgtttc agaaagcact atacttccat tattaaataa agttggtcta
    14581 ggagatattg acaagagacc agtaaaacaa ttttctcttg gaatgaagca acggttaggt
    14641 atagctatct ctttaataaa ttcacctaaa ctacttatat tagacgaacc tactaatggc
    14701 ttggacccaa ttggaattca agaattaagg gaaattatag agtcatttaa atcagaagga
    14761 atgacaatta tgatttcaag ccatatactg tcagaagttg aacatctagc tgattttatt
    14821 ggatttatct atgaaggaaa gattgttctg gaaaaagaat atgacggctc tgagaatctt
    14881 gaagagttat tcaataatca aattttattt gaaaagagga ggtaggggct atggtacaaa
    14941 tttttttatc aaatttattt aaaataaaaa atacgagtat tagcaagttg attcttattt
    15001 ttcctattat ttgtgtgatg ttatctttat tgttttcagc attaggtggt gaaagtatcc
    15061 tacgtttagc tgcggaaaca acagtaaatc agtggggagt tatttggatt aatgttatta
    15121 ttgctgtaaa ttctggtttg ttaaataaat tagaattaga ttctaataag tattctgttt
    15181 ttttatcaag ggatgttgat ttaaaaaagg tagaatatgc cagagttcta ttgatagctc
    15241 ttataaatct aataattagt atgattttaa gcttaatgct tattgtgatt agttttgtcc
    15301 tacctactcc aagtcttatt agtataggta ggatactatt aactatcttg ttaatttggt
    15361 tgactacact atggcaaatc ccgtttattt tatggctatc aagaaaaatt aatgtgtatt
    15421 ttgctatgat tattaatatt atatctccac taattattgg tacaagtttt tctctcttaa
    15481 ataaatggta tttgttccct tatgattggt cgttgaagtt gcttgagcca atgacaagaa
    15541 tgagaataaa tagtatacct tttggagcgg agtttgttcc agactactca cagattttta
    15601 tatcattgtt cctaggaatt gcttttttca tcttactgac caatctattt gctatctctt
    15661 tcaaaaaaca ggtgaaatag tcatgaaatc atatttatgt tctaattttt taaaaatcaa
    15721 aaatacttca tatttactaa tacatatttt agctgcacta ttatttccct tacttctatt
    15781 tttctattgg agtcaacgtg gaggtttaca aagcaggact gtattatttt cctactttca
    15841 aatagtaggt gtacttcttc cttttgttgc aagtattgtg tgcattcaac taaaaaattt
    15901 ggaagaatca tctggaaaat ataaatattt attgggttac tcacagtcga attataagcc
    15961 atttattgta gaattagtat ttctatggtt atgctattgt atagtattaa ttatttcaat
    16021 tactatattt attcttttat tgaaaactat tggtataaat gtatctctca gactacttat
    16081 tttgaatagt ttaatttata tcatttttgc ctatgtaacc tatctgatca atcatattat
    16141 tagctatata tttagtacag gtgtggcatt aggtatttca atggtaggtg ttattgttgc
    16201 agctttttgt gaaacgagcc ttggtgataa ggtatggttt cttattccat gggcatggct
    16261 tttaagaata tcagatactc tatataacca acagaaaatg gcaattgttc cacttattgt
    16321 tatatttttt gtttcatgca cagtagcttt acttcatata cgaatattta aaagttggaa
    16381 tcaagactgt ttgacaagta gttaaataca tctattttgt ttacctaaca ataaatatga
    16441 tttttaagtt ataaatacta tctaacttgt ttaactaaaa aatgactata aaatttcaat
    16501 ggtttactag cttatattta atagacttgt ccaaatagga caattaaaca ttttttaatc
    16561 aaatctcctt tatactgata ttaggagaat gttagatgta tccgcgtatt aggaatttga
    16621 gagaagataa cgatttcact caaaagtttg ttgcaaattt actttccttc tctcatgcaa
    16681 attatgcaaa aattgagaga ggagaggttg ccttaatggc agatgttctt gtgcagtttt
    16741 ctaaactcta taacgtcagt attgattatt tattagggca aacagattat ccgtatcgtt
    16801 ttcataataa ataaaagtct cgccttgatt tgttcattga caactgaata aaccgaggtg
    16861 ggactttctt gtttgtcgag caatttagtg agatacgccg tgataggagc gataattatt
    16921 agctataaaa tagagtggtt ctctatctgc catgaccaca aatagggaca atgatacttc
    16981 tgaacgttca ggctgccagc gtaaaaggcc agtgggtgag attcccatga gtgatttaac
    17041 cagtcatacc cacggaggtt gtaatttttt tgaaaggagt ggaggtgtga aaatagtaac
    17101 atataaaaag cttgatgaat attggttttc tcgaacatac tgcaagaaat attattagag
    17161 aagcaaaaag aatagctata aaaaagttga agaagctcgc gaacttgaac aaaatgcggt
    17221 acaattaagt aaatcgccct ttgacaatag aagacttggt attgctccta aagatattgt
    17281 agaagagtta gtaggtgttc tactctctga agaaagttta gaaagtgaga attaaagatg
    17341 actataaaaa atcaaaaaaa gtacaaaggt gtttattgtg ataagaatgg gaagatattt
    17401 tatcaagctg atcttggtgt agatccagta acaggtaaac gagttcaaaa gaaagccaga
    17461 aagaattagt atggaaagcc atttggtaca atgaaagaag cgtatgatga actggttcgg
    17521 attaggtatg agttcagtaa taatcttagt attgaaaatg acaatatatc gtttgaaaac
    17581 tatatgaata ctatttacct aagagcatat aagcaaaagg ttcaggctgt aacttatgaa
    17641 atagccttac cacatcataa gttatttatt cagtattttg gttctaaact tttaaaggga
    17701 ataacagctt gagattgtga atcttttagt ttacatatta ttgaaaatta tgcaaagaat
    17761 ttatggtcgc gattcaaagc ttgcatgggg tatgctgaaa ggttagacta tatttcaaaa
    17821 gtgccatgta aggctttgga taatcctaaa gggaaacatc ccgacactcc attttggact
    17881 tatattgagt ttcagaattt tataaaatcg tttgatttgc aagattatga agagctacaa
    17941 agatttacaa ctatttggct atattatatg acaggtgttc gtgtaagtga aggtttatca
    18001 ttatgatggg aaaatatcga tttcgaacaa aaattcttga aagttcatac aaccttggaa
    18061 aaggatgaaa atggaaattg gtatcgtaaa gaccaaacaa aaacgccagc aggagaacgt
    18121 cttattgagt tagatgatgt gacaatagta gtattggaaa attggagaag aaatcaagta
    18181 gtcaatactg acacagattt cattatttca cggtttggtg aaccattttg taaatcaacg
    18241 atttgtcgag ttattaagca caaagctcaa gaagtaggag taccagtgat aacgggaaaa
    18301 ggcttgcgac atagtcatgc ttcctatctt attaatgttt tgaaaaaaga cattctgtac
    18361 gttgcaaagt gtatgggaca tgctgataag tctacaactt tgaatacata tagtcactga
    18421 tttaacgcat tagataaaac agtatctgaa gaaataaccc aaaatgtaat aagttcagga
    18481 ttagattcca ttttatacca aaattcctgc caaacaacta attgatataa cgaagcttat
    18541 tatatcgccc atataacagg atgtgttatt gttacagggt acttgcttta tgagcttgtt
    18601 taagctacta aaggtgtgtg ccactatact tccttccaaa tttctgagaa tttttgctat
    18661 aatagtaact atgaaagata taatgataaa aaggtgggtt gtcctttttt ttatggtatt
    18721 aacttgttca gggtttggta aaacagttct cgcagctgat tttcaagtgg gagcagaaca
    18781 tgcgattgtc gtagaggcag actctgggag agttctgtat gagaaagacg ctaagacgcc
    18841 agatgctatt gcctctttga ccaaattggt gacggcttat ctggttttag ataaggttaa
    18901 atcaggccag ctgcaattat cagatcaagt taatctctca gattatgcct tcgagttaac
    18961 aaaagatagg tctttaagta atgtaccttt tgacaaaaaa acttattcag ttcaagacct
    19021 gttgacagct actttggtag caagctcaaa tagcgctgct attgcgttgg ccgaaaaagt
    19081 ggcgggctca gagcctcatt ttgtaaatca aatgagagaa cagctatctc attgggggat
    19141 tacttctggt aaaatcctca acgcttcagg tctgccaaat gaggtgttaa aggataatcg
    19201 ttatcctggt tctgcgttgg aagaagaaaa taccttgagt gctcaagatg ttgcagttgt
    19261 gacgatgcat ttgttagaag attttcctga gattttagag atcaccaaac aaacagaagt
    19321 taattttgct ggcaattcta ttaaaagttt taatcaactc ttaccaggta tggctaaggg
    19381 tagagcagga gtggatggtt taaagactgg gacaacagac ttggcaggtc attgtcttgt
    19441 ggtaacttct attgaaaatg gtatgcgagt gattaccgtt attctaaatg ccgatggatc
    19501 tgataaaaac caaaacacgc ggtttgagca agcaaatcgt ttgttggact atgtggctag
    19561 aacttattgt cgtcgtaaga ttctaaaaaa aggaagtctt gtctcggagc ggtcgctccc
    19621 tattcaagat ggccaagtaa aagaactacc ggtatctgtt accgaagatg taaccatcat
    19681 tttgcaacag ggtgaacaag ttcccaaacc taaacaattt atgatatcag agacttcttt
    19741 actagcaccg attactaaag gtgaagtggt tgcttatttg acgtctccaa gaataacaga
    19801 tcagtcagtg aggtatttaa aggaacctaa gcgtattcct cttaaagcta gccaatctct
    19861 taaaaaagcc tctgacttac agctgtggtg gcgagatttt cttgaaaaaa gacgttaaag
    19921 ataagaataa aataatttta aaacaatcaa aaaaagacag ttatgaaaga attttttcat
    19981 aactgtcttt ttagtttaca gtgagtatga tttggaatga taaaaaaatt gatgttatgg
    20041 ttccattgtt tgtaaaagat tttcaggact catgtttggg aggatacgaa tccggttaag
    20101 agaaagaagt ccattagccg cgctagctat tccttcgtta gtggtaacac ctaatttata
    20161 gttaagtcta gctgcaattt gtaaggtagt gtcattatat ctaccagatg gataagcaat
    20221 ggcaatagtg ttttggttta attgcttatc aagatagtcc tttgaatctt tcatttcagt
    20281 tgtttgaaca tcgggactag cctgctctag atcaggatga ttcactgtat gatcttggaa
    20341 agacataccg aattgtttca tttctttcat ctgcttaaga gtcagattag cagcgcttcc
    20401 tatttcggtt agtcctgtaa taacattatt ggtggcttta gcatcatatt tttttaagat
    20461 tgggtaagca acgttgtaga aatcaatcat actgtcatca aatgttagcc atacaacttt
    20521 tttagcaggc aattcattat ttgaaagagc gcggtagacc tcttcaggac tcaaaaaata
    20581 gtagccctcg tctttcattt tttgaagttg ttgatcaaag agatctgggt tgacaatcaa
    20641 attagcatta gccgtctctt caggggacat tacatgaata gcatgataca ttaaaattgg
    20701 gatcttgaca ggtgtttctt gcttggtcca agttaattta gaatgggcct ttttttgaga
    20761 ttttgaggaa tcgaaatgag tcttcttaag ttttgtcgtt tctttttttg ccgtcttttt
    20821 gtgatgaaaa agacttattt taggtacaaa ttgagagaca tctgacaagc cgaatttgtt
    20881 agacccttga tataaataga aaatgagggt caaaccaaga atgcaggcga ataatagtat
    20941 aaggttaatg agagtgaagg cttttctttt ttggcgccgt ttatgtctat tgttcattac
    21001 ttgctccttt tttgatataa gtacctctat tgtaacattt tttagagaaa agaaggcttg
    21061 aagtcagcct tttttcttgt tttttggaag aacgtgtatt aaggagtaaa aaaaatattg
    21121 tcacagataa cactttagga ggtagcaaag actatgtggg gctggtattc ttatcacgta
    21181 aaaaaagcaa tgaagataaa agtgtagtgt actttagaac ttatttgagt gtttggtgag
    21241 gataataaaa aaaagggtac tactctaggt tatctgctgt cacttttttt tatatatggt
    21301 atactaaaga tagatgtgag gtatgaaatg acttatgaag agactctaga atggattcat
    21361 gatcacttag ttttcggcat caagccaggc ttaaaacgta tgctttgggt tcttggacag
    21421 ttaggaaatc cccagaaaaa tgtcaaggga gtgcatatcg ttggaactaa cggaaaaggt
    21481 tccacggtca atcatttaca acacatcttt acgacagctg gttatgaagt ggggaccttt
    21541 acctctcctt atattatgga tttcaaagaa cgtattagca taaatggtcg aatgatttca
    21601 gaaaaagatt tggttattgc tgctaatcgt attcgtcctt tgacagagcg tctagttcaa
    21661 gagactgatt ttggagaggt aactgaattt gaagtgatta ctttgatcat gttcctctat
    21721 tttggtgata tgcatcctgt agatattgct attattgaag caggccttgg cggtctttat
    21781 gattcgacca atgtttttca agcaatggtt gtggtctgtc cctcaattgg tttagatcac
    21841 caagctattt tgggagagac ttacgctgat attgccgccc aaaaagctgg tgttttagaa
    21901 gggggcgaga cccttgtttt tgccgttgaa aacccgtcag ctcgagaggt ttttttaaca
    21961 aaagctgagc aggttggtgc ttcaatctgg gaatggcaag agcagtttca aatggctgag
    22021 aacgcatctg actatcgttt tactagtcct ttaggagtta tttcagatat tcacatcgct
    22081 atgccaggac accaccaagt gtctaatgca gctttagcta ttatgacttg cttaaccttg
    22141 aaagaccgct atcctaggct aactccagat catataaggg aaggtctggc aaatagtttg
    22201 tggctaggcc gtaccgaatt attagcacca aatctaatga ttgatggagc tcataacaat
    22261 gaaagcgttg ctgctttggt agccgtctta aaaaataact acaatgataa aaagcttcat
    22321 attttgtttg gtgcgatcga caccaagcct attgtagata tgttggttgc tcttgagcaa
    22381 ataggtgact tgcaggttac tagctttcat taccctaacg cctatccatt ggaaaaatac
    22441 ccagaacgct ttggtagggt tgctgatttc aaagatttct tggccctgcg taagcatgct
    22501 aaagcagatg actttttcgt gattacagga tcactatatt ttatttcaga aattagacgg
    22561 tattggaaaa aacatattga aaaaagcgtt cttttaaccc attaaaaaag agaaaatgaa
    22621 aaggaaaagg aaaaaactaa tgaaaaggga aagactaatg tctattaata aggaaaaagc
    22681 agaagcagcc atttatcagt ttttagaggc tattggtgaa aatccaaatc gggaaggtct
    22741 tcttgacacg cctaaacggg ttgctaaaat gtatgcggag atgtttttgg gactgggaaa
    22801 agatcctaaa gaggagttta cggctgtctt taaagaacac catgaggatg tggtgattgt
    22861 caaagacatt agtttttatt ctatatgtga acaccactta gttccttttt atggcaaggc
    22921 tcatatcgct tacttaccaa gtgatggtcg agtaacaggt ttgagtaaat tagcgcgagc
    22981 agttgaagta gctagtaaac gacctcaact ccaagagcgt ttgacctctc aaatagcgga
    23041 tgctttagtt gaagccctca atcctaaagg gactttagtt atggtagaag cagagcacat
    23101 gtgcatgacc atgcgaggca tcaaaaagcc aggtagtaaa accattacca ccactgccag
    23161 aggcttatac aaggaaagtc gtgctgagag gcaagaagtt atttctctaa tgacaaaaga
    23221 ttaggagaac ttatgaaaat tggaaagttt gtgattgagg gcaatgcggc tatcatgggg
    23281 attttaaatg tgactccaga ttctttttca gatggggggt cttacactac tgtgcaaaaa
    23341 gcattagatc acgttgagca aatgattgct gatggtgcta aaatcatcga cgttggtgga
    23401 gaatcaacac gtccaggttg ccaatttgta agcgctgccg atgaaattga ccgggtggtt
    23461 cctgtgatca aggctatcaa agaaaactat gatattctaa tcagcattga tacctataaa
    23521 accgaaacag ctagagcagc tttagaggcg ggtgccgata ttctcaatga tgtttgggca
    23581 ggtttgtacg acggacaaat gtttgcctta gcagccgagt acgatgcgcc tatcatcttg
    23641 atgcataacc aagacgaaga agtttatcaa gaggtaacac aagacgtttg tgattttcta
    23701 ggcaatagag cacaagcagc tcttgatgct ggcgtgccaa aaaacaatat ttggattgat
    23761 ccaggatttg gatttgccaa atctgttcaa cagaatatgg agttattaaa aggattggac
    23821 cgcgtctgtc agttgggcta tcctgtcttg tttggtattt cgagaaagcg tgtcgtagat
    23881 gccttgttag gcggcaacac caaagctaaa gagcgagacg gagcgacagc agccttgtct
    23941 gcttatgccc ttggaaaagg ctgtcagatt gtacgcgtac acgatgtcaa ggctaatcaa
    24001 gacattgtgg ctgtgttgag ccagttgatg tgaggactta tggataaaat cgtattagaa
    24061 ggttgccgtt tttatggcta ccatggagcc tttaaagaag aacagaccct tgggcaaatt
    24121 tttctggttg atttagaatt gtcagtcgat ttgcaagcag cttctttgtc agaccaatta
    24181 acagatacgg tccattacgg gatggtgttt gacagtgtcc gtcagttagt
//
