LOCUS       NZ_CP035431            23767 bp    DNA     circular CON 15-OCT-2024
DEFINITION  Streptococcus pyogenes strain emm105 chromosome, complete genome.
ACCESSION   NZ_CP035431
VERSION     NZ_CP035431.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1800116)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1800116)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005161125.1-RS_2024_10_13
            Annotation Date                   :: 10/13/2024 02:15:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,766
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 39 of 77
            Pseudo Genes (incomplete)         :: 46 of 77
            Pseudo Genes (internal stop)      :: 21 of 77
            Pseudo Genes (multiple problems)  :: 21 of 77
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:05:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 578244
            Orig. end                         :: 602011
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035431.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..23767
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="azole-containing-RiPP"
                     /region_number="2"
                     /rules="((YcaO or TIGR03604) and (((thio_amide and (PF06968
                     or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N
                     or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or
                     PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or
                     thiostrepton or TIGR03602)"
                     /tool="antismash"
     cand_cluster    1..23767
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((YcaO or TIGR03604) and (((thio_amide
                     and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /kind="single"
                     /product="azole-containing-RiPP"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23767
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13767](+)"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..13767
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
     misc_feature    complement(1122..1133)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            complement(1211..2635)
                     /locus_tag="ETT68_RS03175"
                     /old_locus_tag="ETT68_03165"
     CDS             complement(1211..2635)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019875.5"
                     /locus_tag="ETT68_RS03175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03165"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_227876937.1"
                     /transl_table=11
                     /translation="MSSKSLLIEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLY
                     QTSIFLKMLCFLIEPGELSLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNLVVG
                     PEYRIRFLIALLHYQFGMTIYDFDKTSMNKVVSLIINSNQAITLNDASKAPYEFSYFAI
                     LISLIWKRRHDNLGIPQTDAFKHLKKLSIYHDIKMTSQEIIGKWYHPDLTDEDLDYIFL
                     CFCTTNNPFHKDKWTPKKVKELFELVMTKTIGKTLKASLRPLLGDNILNSLPFKRILVS
                     FSRLFISNLQVLLPDIHLFHYLRRQQKRNKSFYNTLKTIVEEWMSAEGIVGKLPSYHLL
                     LFTIQLEELLKTYLPPIPVYLLTNNTAALDLMTNALSIYFPPAIATVMPVNVEIIPFKD
                     IVKEKQSVIIADRQYLNLIQHLYQNQGHLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDE
                     FIVTLLDTYHKNLSSP"
     misc_feature    2638..2647
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            3233..9136
                     /locus_tag="ETT68_RS03180"
                     /old_locus_tag="ETT68_03170"
     CDS             3233..9136
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880379.1"
                     /locus_tag="ETT68_RS03180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03170"
                     /product="DUF1542 domain-containing protein"
                     /protein_id="WP_227876936.1"
                     /transl_table=11
                     /translation="MFLKHQDVKQKNWRMRKVKKLFVSSCMLLTVGLGVAVPTAFSQSN
                     GVMVVKAAEAEELPDDLMNFKGTWEVSADGSSGRFFSKGATDSYVFHLIPAKDVKNPGW
                     HEHNEVKDSYIKITKDSIAARYKPKTTAPYSVAFKVNTKSLIKDHDYKITFEQGDISSG
                     ITVDYGIGSPFSKDVKDSFNISDESKYASNVKIEGEERGFINRKPGAKTISFRTLKEGA
                     MSLVLLSKVEKKPQGDLDVEFKNFKIIDVTNPSQLDKGVAYVGNKNVELTLKSDNGRTN
                     FEGDEISLFKPNGDLLKKIEVKKGQNPISITLTEEEAKALKNKEQLKVSIKQKQSKKTS
                     DDFFFEVGIDPEVKAKQQEKLLELDKVKKQIEDSINGDAWLPEKPEGEKPVQNTNKELQ
                     LQELNKKYQMAKEAIESATTLEKVTEKYNEYTFEGEKEKYPNSLRNQYTRGDKDKEIEK
                     AKKSLGDLSDKVNGKIEEDKWLSDEVKKKQQQELEARKQKVNDSLKGSDSLKSLRETVE
                     KASSKNQKKPESFEDVYVPGNEETEKTKVRDILQKTYQKTEQNIETDPWLSPEQKKAQK
                     ENAKARLDAGLKAVETTESLDKLKEVESDFLDKEKAESIPSQHQAGTPEVARKTFLDNF
                     EKEAKKEIESIKNDATLTDAEKATAKAKVEAQLQEAKDKAKESKSFDDLKNIQDKFNSE
                     LPHTPGKPLKDQQSDAIAELEKKQQEIEKAIEGDKTLPRDEKKKQIADSKERLKSDTQK
                     VKDAKNADAIKKAFEEGKVDIPQAHIPGDLNKDKEKLLAELKQKADDTEKAIDSDKALT
                     AKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIEDTHVKGNLEGIKNKAI
                     EDLKKAHTETVAKINGDDTLDKATKEAQVKETDKALAAGKDAITKADDADKVSTAVTEH
                     TPKIKASHKTGDLKKAQVDANTALDKAAEKEREEINNDITLTAKDKEQQLKEVETALTK
                     AKDNVKAAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVAKLEEAAKATKDKISA
                     DPTLTSKEKEEQSKAVDAELKKAIEAVNAADTADKVDDALGKGVTDIKNQHKTGDPVEA
                     RREAHGKELDRVAQETKGAIEKDPTLTTEEKAKQVKDVDVAKERGMAKLSEAKDADALD
                     KAYGEGVTDIKNQHKSGDPIEARRGLHNKSIDEVAQATKDAITADTTLTEAEKETQRGN
                     VDKEATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGKGLDVRKDEHKKALEAVAKR
                     VTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKDADEADKAYGDGVTAIENAHV
                     IGKGIEARKDLAKKDLAEAVAKTKALIIEDKTLTDDQRKEQLSGVDTEYAKGIENIDVA
                     KDAAGVDKAYSDGVRDILAQYKEGQNLDDRRNAAKEFLLKEADKVTKLINDDPTLTHDQ
                     KVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGIENINNQYQHGDGVDVRKATAKGD
                     LEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKNTAIAAVDKATTADGVNQELGKGIT
                     AINKAYRPGEGVKARKEAAKADLEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKNTA
                     IAAVDKATTADGVNQELGKGITAINKAYRPGEGVKARKEAAKADLEKEAAKVKALIAKD
                     PTLTQADKDKQTAAVDAAKNTAIAAVDKATTAEGINQELGKGITAINKAYRPGEGVKAR
                     KEAAKADLEKEAKETKALISGDRYLSETEKAAQKQAVEQALAKALGQVETAKTVEAVKL
                     AENLGTVAIRSAYVAGLAKDTDQATAALNEAKQAAIEALKQAAAETLAKITTDAKLTEA
                     QKAEQSENVSLALKTAIATVRSAQSIASVKEAKDKGITAIRAAYVPNKAVAKSSSANHL
                     PKSGDANSIVLVGLGVMSLLLGMVLYSKKKESKD"
     gene            complement(9322..>9438)
                     /locus_tag="ETT68_RS03185"
                     /old_locus_tag="ETT68_03175"
                     /pseudo=""
     CDS             complement(9322..>9438)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /locus_tag="ETT68_RS03185"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03175"
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="QETFYSLEQLTLKTKEEVHWWNYYHLHSNLNYQTPITK"
     gene            10001..10162
                     /gene="sagA"
                     /locus_tag="ETT68_RS03190"
                     /old_locus_tag="ETT68_03180"
     CDS             10001..10162
                     /codon_start=1
                     /gene="sagA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03602"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /locus_tag="ETT68_RS03190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03180"
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="WP_002985285.1"
                     /sec_met_domain="TIGR03602 (E-value: 1.7e-29, bitscore:
                     98.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQGG
                     SGSYTPGK"
     misc_feature    complement(10169..10186)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            10384..11334
                     /gene="sagB"
                     /locus_tag="ETT68_RS03195"
                     /old_locus_tag="ETT68_03185"
     CDS             10384..11334
                     /codon_start=1
                     /gene="sagB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: PF00881"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /locus_tag="ETT68_RS03195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03185"
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="WP_010922108.1"
                     /sec_met_domain="PF00881 (E-value: 1e-32, bitscore: 110.2,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVLK
                     TSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVYES
                     QEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASIRDG
                     ASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEENVRDF
                     AEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATALTYGSI
                     DIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     aSDomain        11331..11600
                     /aSDomain="Cytolysin_C_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing discrete C protein in a
                     SLS/cytolysin LAP cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_ETT68_RS03200_Cytolysin_C_RRE.1"
                     /evalue="2.40E-42"
                     /identifier="RREFam014.1"
                     /label="ETT68_RS03200"
                     /locus_tag="ETT68_RS03200"
                     /protein_end="90"
                     /protein_start="0"
                     /score="134.1"
                     /tool="antismash"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIVY
                     QEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLML"
     gene            11331..12389
                     /locus_tag="ETT68_RS03200"
                     /old_locus_tag="ETT68_03190"
     CDS             11331..12389
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03603"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cytolysin_C_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /locus_tag="ETT68_RS03200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03190"
                     /product="streptolysin associated protein SagC"
                     /protein_id="WP_010922109.1"
                     /sec_met_domain="Cytolysin_C_RRE (E-value: 1.5e-40,
                     bitscore: 134.1, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03603 (E-value: 4e-140, bitscore:
                     463.6, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIVY
                     QEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMKIL
                     MGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQTDV
                     SSRLDALEHRRNMNCLSDALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIGFVDG
                     PFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQVLPATQDVSQAYLPLLNVLM
                     NLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLKYEDQQI
                     STREIVKKLLDE"
     gene            12409..13767
                     /locus_tag="ETT68_RS03205"
                     /old_locus_tag="ETT68_03195"
     CDS             12409..13767
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /locus_tag="ETT68_RS03205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03195"
                     /product="YcaO-like family protein"
                     /protein_id="WP_115231455.1"
                     /sec_met_domain="YcaO (E-value: 2.2e-62, bitscore: 208.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 2.1e-110, bitscore:
                     365.7, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKSI
                     TGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRIVY
                     ASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFFEDA
                     EMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLDAAMCNSLIEYIQIDSMMLSW
                     YTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILKNKYDE
                     GPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLFLDLDSN
                     VFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKVSPNAVFL
                     DITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHPMP"
     gene            13742..14413
                     /locus_tag="ETT68_RS03210"
                     /old_locus_tag="ETT68_03200"
     CDS             13742..14413
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /locus_tag="ETT68_RS03210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03200"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_002994083.1"
                     /sec_met_domain="Abi (E-value: 1.2e-08, bitscore: 32.0,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRAT
                     LIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVFIVLIELVFLHGLRCWQKKQW
                     LPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSFCYA
                     LNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKKKKEV
                     T"
     gene            14410..15093
                     /locus_tag="ETT68_RS03215"
                     /old_locus_tag="ETT68_03205"
     CDS             14410..15093
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /locus_tag="ETT68_RS03215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03205"
                     /product="SagF family protein"
                     /protein_id="WP_032464275.1"
                     /transl_table=11
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLMALSYQDFLDAL
                     LSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVLIL
                     IFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQSLTS
                     LATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVTYLMI
                     SCKEY"
     gene            15118..16041
                     /locus_tag="ETT68_RS03220"
                     /old_locus_tag="ETT68_03210"
     CDS             15118..16041
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /locus_tag="ETT68_RS03220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03210"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_136275703.1"
                     /transl_table=11
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKST
                     LINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVGLFGSLY
                     GLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDEPTV
                     GIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPKFELE
                     KRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQLTQANI
                     TFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            16050..17177
                     /locus_tag="ETT68_RS03225"
                     /old_locus_tag="ETT68_03215"
     CDS             16050..17177
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /locus_tag="ETT68_RS03225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03215"
                     /product="SagG family ABC transporter permease subunit"
                     /protein_id="WP_136113567.1"
                     /transl_table=11
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFNT
                     ATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGALTA
                     VLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKRSVD
                     YVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTGVSPS
                     LLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAVGIGLG
                     ISIKNEAFLTVVSNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDSLFYLIF
                     GGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            17174..18292
                     /locus_tag="ETT68_RS03230"
                     /old_locus_tag="ETT68_03220"
     CDS             17174..18292
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985264.1"
                     /locus_tag="ETT68_RS03230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03220"
                     /product="ABC transporter permease"
                     /protein_id="WP_012560577.1"
                     /transl_table=11
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQAK
                     IGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSDKV
                     LQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQLNY
                     DVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSNLSRF
                     RYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFGLLIGA
                     ITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIAIFQSGG
                     TLSQAWPYLLALMGTALALISFSSLLLKPTRL"
     misc_feature    complement(18299..18312)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.45"
                     /tool="antismash"
     misc_feature    complement(18525..18543)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.83"
                     /tool="antismash"
     misc_feature    18527..18541
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.65"
                     /tool="antismash"
     misc_feature    complement(18529..18540)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 18.98"
                     /tool="antismash"
     misc_feature    complement(18646..18661)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.72"
                     /tool="antismash"
     gene            18863..21595
                     /locus_tag="ETT68_RS03240"
                     /old_locus_tag="ETT68_03230"
     CDS             18863..21595
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /locus_tag="ETT68_RS03240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03230"
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="WP_110410777.1"
                     /transl_table=11
                     /translation="MINKKCIISVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKRE
                     SVISDKSNFPVISPYLASVDFGERKTPLPTPDKGVKVTTEQSIAQVRKGPEERTYTVTG
                     KITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQQVT
                     AHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDDSGKS
                     IVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKKVTSSN
                     SDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDGDLATSD
                     GIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKTGTAQVPS
                     PLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGPMKNKEIYV
                     LPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAGPVSYSYGNY
                     KVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSSTKDEKVKRIAE
                     SFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGPTYRYVDIAPEN
                     NVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNRELNLSVGRIDPTNAAWKD
                     VRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQRRHVLANMLAQFAK
                     EGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRYSYFHQGNNQTLDNI
                     LVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKENDKAESSKQSVKAKKT
                     SKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      21760..21875
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            21873..22373
                     /locus_tag="ETT68_RS03245"
                     /old_locus_tag="ETT68_03235"
     CDS             21873..22373
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /locus_tag="ETT68_RS03245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_03235"
                     /product="QueT transporter family protein"
                     /protein_id="WP_002985256.1"
                     /transl_table=11
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMMN
                     FLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLFNG
                     IFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKLSQR
                     ISF"
     misc_feature    complement(23599..23613)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.38"
                     /tool="antismash"
     misc_feature    23625..23636
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.03"
                     /tool="antismash"
ORIGIN
        1 caatcgtatg tggcttggtt gaagctgttt gcgattgcct ttttcgtttc tcatagaagt
       61 ctgtgatatg acaaggattg gtatgactag cagaagcaat gtcatggata cacttgaaaa
      121 gaatctttgg agcataagga ttcccacgtt tggtaatgtg cttttgagct agatagttgc
      181 cagactcata atgtctaaag tcaatcccaa tgaaagcatt aatttgatta gcagattgaa
      241 aacgacgaat agcaccgagt tcgccaataa tacttgttgc agttgtttct gcgatacttg
      301 gaatagagcg tagaatcttg tcttctggta aaggttgagc caacgccacc atcttatcaa
      361 aaatagcttg acgacgttca gaaagtctaa gtaattcttt ggcatagcaa cgaacctctt
      421 ctatcatcgg agagtttttc gtaacagtaa aataagactg cttggctaat gagacaagtt
      481 tttccactaa gtagatccca cgcttgtcag agatacgttt agaggtagat tgacggatga
      541 tagctgagaa tgctttgtca gctagctcta gtacaaagct attacatgga aaagccgtta
      601 ccacattcca gtattactca ccagttggtg caaataagat gtgttccagt tcaggaaaag
      661 tgacttgtaa gatcttgtga aggcgattct taactctcga acaatattat cctcagtcaa
      721 gttttgatag aagcgactca agtcacgtag cagctgataa acttcttcct gaacgtaagt
      781 tggttttcta actagaataa attgagactg agccaatttt tcagtgtcta acttatccgc
      841 tttacgcacc tgtagactat ctagttttct aaaggattga gttgtgtata agcgtagcca
      901 tttttctcaa ggaaggactg aagccgacga gaatagactc ctgttgtgat ggtctccctt
      961 ttgcctttta aacaatagta tagccgtttt tcaaaatgtt attaatgatt ttttccttta
     1021 ttctagaaga ataagtctga tactgtttct tcttaataat ctcaatgccc taacaatcca
     1081 tcagtcgaac ggtatttctc aaattactgc agttgactct tgcgttcagt tccctcacct
     1141 tgtggatgcc gtttagaaca atattagctt gctgagcctt ttggtaatgt gaacaaagca
     1201 tctttatcac tcaaggagaa gacaggttct tgtggtaagt gtccaacaag gtcacgataa
     1261 actcatcgta tcgtttttgg cggaagtcta gaaatgcctt atgaatgtaa gcctcagaaa
     1321 cgtctctaaa actaaataag aaatacaaga acaaatgccc ttgattctgg tacaaatgct
     1381 gtatcaggtt caggtattgc ctatctgcga taatgacact ctgcttttcc ttaacaatat
     1441 ctttgaaagg aatgatttcg acattgacag gcataaccgt tgcaatagct ggcgggaagt
     1501 aaatcgagag tgcattggtc atcaaatcca gcgcagcagt gttattggtc aaaagataga
     1561 caggtatcgg tggcaaatat gtttttagga gttcctctaa ctgtatcgtg aagagcagta
     1621 aatgatagga cggcagtttc cccacgatgc cttctgcgct catccattcc tcaacgatgg
     1681 tttttagagt attgtagaag ctcttgtttc gtttttgctg tcttctgagg taatggaaca
     1741 agtgtatatc aggtaacaaa acttgaagat tactgataaa gagacgtgag aaggaaacca
     1801 aaatccgttt gaaaggaagg gagtttagga tattatcgcc tagtagtgga cgcagggatg
     1861 ctttcaaggt ttttccaatt gtttttgtca tcaccaactc aaacaattct ttcacttttt
     1921 taggtgtcca tttatcctta tggaaaggat tattggtggt gcaaaaacat aagaaaatat
     1981 aatccaaatc ctcatcagtt aaatcaggat ggtaccattt tccgatgatt tcctgactgg
     2041 tcattttgat atcgtgatag atagacagtt ttttcaagtg tttgaatgca tctgtctgag
     2101 ggatccccaa gttatcatgt cgccgtttcc atataaggga tatcagaatg gcaaagtatg
     2161 aaaattcata aggggcttta ctggcatcat taagtgtgat ggcttgattg gagtttataa
     2221 tcagcgaaac cactttattc attgatgttt tatcaaaatc ataaatggtc ataccaaact
     2281 gatagtgcaa caaggcgatc agaaagcgga tacggtattc tggtccaaca acaaggtttt
     2341 gccgaacgtt taaacctacc tttttcaagt atttccgaca attggtcctg atacggtaag
     2401 cagtggcttg ggaaatatac tctcgtttaa tgaagtcgtg taggcttagc tctcctggtt
     2461 caattaaaaa acataacatc tttaagaata tcgaggtttg atagagtttc ttaaaataag
     2521 gcattttaag ttggtaggtc ttatagggga tagaaacagt gtcgtcacaa atggttaagg
     2581 tgagggtgtc ttctaaatcc ttaatcattt caatgagtaa ggatttagaa gacacgttgt
     2641 tttttgagtt cggtttcctg acacaactgg tctatggaga cttcggaaac gtctaacaaa
     2701 atgttgagca gtgataattt atcttgtaat ttggtttcta gatgaaggtg taacataact
     2761 gaactccttt catttttata gcagaaactc gtatttattt atttaattat cacataatta
     2821 aataaaacag tcaaataatt caatggtgac gcgtgtttaa ggagtattga gaatgtcaga
     2881 tataagcaaa atgcagctga atattcttaa ttttaggtat gaaaaagcgt cgggattgat
     2941 aaaaaatcta ttgaattggg taaatttaag ttgatataat aagttgatta gaaaaattaa
     3001 caattcattt aaattctaat cagctgtaac gttccggggc gtgttaaact gtctgaacgt
     3061 agaaaataaa ggtttctacg gtgactaaga taagttaagg aggattcctt aaattctgtg
     3121 ataggaacgt aaactctcta caagagtaga gcacgtcatc agtcagtctg ggtgtctact
     3181 ggctgacaac gtcaaataag ataggcatga aaaaaataag ggggaacaca gaatgttttt
     3241 aaaacaccaa gatgtgaaac aaaaaaattg gagaatgcgt aaggtcaaaa aactctttgt
     3301 tagttcatgt atgcttttaa cagtcggcct cggagttgcc gtacctactg cattcagcca
     3361 atctaatggc gtgatggtcg taaaggctgc ggaagctgaa gaactgccag atgatttgat
     3421 gaattttaaa ggaacttggg aagtatctgc cgatggctct tctggtcgct tcttttcaaa
     3481 aggtgctacg gatagttatg tgttccacct tattccagct aaagatgtta aaaatccggg
     3541 atggcatgaa cataatgaag ttaaagatag ctatatcaaa ataaccaaag attctattgc
     3601 agctagatat aaacctaaaa caaccgctcc atattctgta gcgtttaagg tcaacactaa
     3661 atctttaata aaagatcatg actataagat aacttttgaa caaggtgaca tttcatcagg
     3721 tataaccgtt gattatggca taggcagtcc ttttagtaaa gatgtaaaag attctttcaa
     3781 catttcagat gagagtaaat atgcttctaa cgtaaaaata gaaggtgagg aaagaggttt
     3841 tataaataga aaaccaggag ctaaaacaat atcttttaga actttaaaag aaggcgctat
     3901 gtctcttgta ttgttatcta aagttgagaa aaaacctcaa ggagatcttg atgtcgagtt
     3961 taaaaacttc aaaattattg atgttacaaa cccaagccaa ttagataaag gagttgctta
     4021 cgttggaaac aaaaatgtag aattgacatt aaaatcagac aatggaagaa caaacttcga
     4081 aggtgatgag ataagccttt tcaagcctaa tggagatttg ttaaagaaaa ttgaagttaa
     4141 aaaaggacaa aatcctatat caataacttt aacagaggaa gaagctaaag ctttaaaaaa
     4201 caaagaacag ttaaaagtaa gcattaaaca aaagcaatca aaaaagactt ctgatgactt
     4261 tttctttgag gtaggaattg atcctgaagt taaagcaaag caacaagaaa aactcttgga
     4321 actagacaaa gttaaaaaac aaattgaaga ctcaatcaat ggtgatgcat ggctaccaga
     4381 aaaaccggaa ggcgaaaaac cagtacaaaa tactaataaa gaattacagt tacaagaact
     4441 gaacaagaag tatcagatgg ccaaagaggc aattgaaagt gctacaactc ttgaaaaagt
     4501 tactgaaaaa tataacgaat atacattcga aggagaaaaa gaaaagtatc caaattcttt
     4561 acgtaaccaa tatactcgag gagataaaga taaagaaata gaaaaagcta aaaaatcact
     4621 gggtgattta agcgataaag tgaatggaaa gattgaagaa gataagtggc tatctgacga
     4681 agttaaaaaa aaacaacagc aagaattaga agcgcgtaaa caaaaagtaa acgactctct
     4741 taaaggatct gatagtttaa aatcattgag agaaacagtg gaaaaagctt cttctaaaaa
     4801 tcagaaaaaa ccagaatctt ttgaagatgt ttatgttcct ggaaatgaag aaactgaaaa
     4861 aacaaaagtt cgtgatatac ttcaaaaaac ttatcaaaaa acagagcaaa atattgaaac
     4921 agatccatgg ttgagtccgg aacagaaaaa agctcaaaaa gaaaacgcta aagcaagatt
     4981 agatgcaggt ttgaaagctg tcgaaactac agaaagctta gataaactta aagaagttga
     5041 gagtgacttc cttgataaag aaaaagcaga atctattcca tctcaacatc aagcaggaac
     5101 tcctgaagtt gctcgcaaaa ctttcttaga taactttgaa aaagaagcta aaaaagagat
     5161 tgaatcaatt aaaaatgacg ctacattaac agatgctgaa aaagcaacag ctaaagctaa
     5221 agtagaagca caacttcaag aagctaaaga taaggctaag gaatcaaaat cgtttgatga
     5281 tttgaagaac attcaagata agttcaattc tgaattacca cacacaccag gtaagccgct
     5341 taaagatcaa caatctgatg ctattgcaga attagagaag aagcagcaag aaattgaaaa
     5401 agctattgag ggtgataaaa cattaccaag agacgaaaaa aagaaacaaa ttgctgactc
     5461 taaggaacgc ttaaaatctg acacgcaaaa agttaaagat gctaaaaatg ctgatgctat
     5521 taaaaaagca tttgaagaag ggaaagtgga tattcctcaa gcacatatcc caggtgattt
     5581 gaacaaggat aaagaaaaac ttcttgcaga gttgaagcaa aaagcagatg atactgaaaa
     5641 agcgattgat tccgataaag ctttgacagc gaaagaaaaa gaagagcaaa aagcgaaaac
     5701 aaaagaagaa cttcaaaaag caacagaagc tgttggagca attgacaatc gtgaagaact
     5761 agataaaaaa gttccagaac ttaagaaagc tattgaagac actcacgtta aaggtaatct
     5821 tgaaggtatt aagaataaag ctattgaaga tcttaaaaaa gctcatactg aaacagttgc
     5881 taagataaat ggtgatgata cccttgacaa agctactaaa gaagctcaag tgaaagaaac
     5941 tgacaaagct ttggcagcag gtaaagatgc gatcactaaa gcagatgatg ctgataaagt
     6001 aagtacagct gttacagagc acacgccaaa aattaaagca tcacataaaa ctggtgatct
     6061 taaaaaagct caagtagatg ctaacacagc tcttgacaaa gcagctgaaa aagaacgtga
     6121 agaaatcaat aatgatatca cattaacggc aaaagacaaa gaacaacaac tgaaagaagt
     6181 tgagacagct cttactaaag ctaaagataa cgtgaaagct gctaagacag cagacgctat
     6241 caatgacgca cgtgataaag gcgtagcaac aattgatgcc gtccataaag caggtcaaga
     6301 cttaggtgct cgtaagtcag gtcaagtcgc taaacttgaa gaagcagcta aagcaacgaa
     6361 agacaagatt tcagctgatc caactttgac aagcaaagaa aaagaagagc aatctaaagc
     6421 tgttgacgct gaacttaaga aagcgataga agctgttaac gcagctgaca cagctgacaa
     6481 ggttgacgat gctcttggta aaggtgtcac agacatcaag aaccaacata agacaggtga
     6541 ccctgtcgaa gctcgtcgtg aggctcatgg taaggaactt gatagagtcg ctcaagaaac
     6601 taaaggtgcg attgaaaaag accctacttt gacgactgaa gaaaaagcta aacaagttaa
     6661 agacgtagat gttgctaaag aaagaggcat ggctaagctt agtgaagcta aagatgctga
     6721 tgctttagac aaagcttacg gtgaaggtgt tacagacatc aagaaccaac acaagtcagg
     6781 tgaccctatc gaagctcgtc gtggattaca caacaagtca atcgacgaag tggctcaagc
     6841 aactaaggac gctatcacag cagatacgac tttgactgaa gccgaaaaag aaacacaacg
     6901 tggcaatgtt gataaagaag caactaaagc taaagaagaa cttgctaagg ctaaagatgc
     6961 tgatgcttta gacaaagcgt acggtgacgg tgtaaccagc atcaagaacc aacacaagtc
     7021 aggtaaaggt cttgacgttc gtaaagatga gcacaagaaa gctcttgaag ctgttgctaa
     7081 acgtgtcact gctgaaattg aggctgatcc aaccttaaca ccagaagtga gagaacaaca
     7141 aaaagcagag gttcaaaaag agcttgaact tgcgactgat aagattgctg aagctaaaga
     7201 tgcagatgaa gcagacaaag cgtacggtga cggtgtcaca gcgatcgaaa atgcccatgt
     7261 tattggtaaa ggtatcgaag ctcgtaaaga ccttgctaag aaagaccttg ctgaagctgt
     7321 tgctaagaca aaagctctca ttattgaaga caaaacgctt actgatgatc aacgtaaaga
     7381 acagttatca ggtgttgata cagagtatgc taaaggtatc gagaatattg atgtagctaa
     7441 ggatgctgca ggtgttgata aagcatatag tgacggtgtt cgtgacatcc tggcacagta
     7501 caaagaagga caaaaccttg atgatcgtcg taatgctgcc aaagaatttc ttcttaaaga
     7561 agcagacaaa gtgacgaaac taatcaatga tgatccaacc ttgactcatg accaaaaagt
     7621 tgatcaaatc aacaaagttg aacaagctaa gttagacgca atcaagtctg ttgatgacgc
     7681 tcaaacagct gatgctatca atgatgctct tggtaagggt attgaaaaca tcaacaacca
     7741 ataccaacat ggcgatggcg ttgatgttcg taaagcgact gccaaaggcg atcttgaaaa
     7801 agaagctgct aaagtgaaag ctcttattgc taaggatcca accttgactc aagctgataa
     7861 agacaaacaa acagcagcgg ttgacgcagc taagaacaca gcaattgcag cggttgataa
     7921 agcgacaaca gcagatggtg ttaaccaaga acttggtaaa ggcatcacag ctatcaacaa
     7981 agcttaccgt ccaggtgaag gtgttaaagc acgtaaagaa gccgctaaag ctgatcttga
     8041 aaaagaagct gctaaagtga aagctcttat tgctaaggat ccgaccttaa ctcaagctga
     8101 taaagacaaa caaacagcag cggttgacgc agctaagaat acagcaattg cagcggttga
     8161 taaagcgaca acagcagatg gtgttaacca agaacttggt aaaggcatca cagctatcaa
     8221 caaagcttac cgtccaggtg aaggtgttaa agcacgtaaa gaagccgcta aagctgatct
     8281 tgaaaaagaa gctgctaaag tgaaagctct tattgctaag gatccgacct taactcaagc
     8341 tgataaagac aaacaaacag cagcggttga cgcagctaag aatacagcaa ttgcagcggt
     8401 tgataaagcg acaacagctg aaggcattaa ccaagaactt ggtaaaggca tcacagctat
     8461 caataaagct taccgtccag gtgaaggtgt taaagcacgt aaagaagccg ctaaagctga
     8521 tcttgaaaaa gaagctaaag aaactaaagc tcttatttca ggagaccgtt acttgagcga
     8581 aactgaaaaa gcagcccaaa aacaagctgt tgagcaagct cttgcgaaag cacttggtca
     8641 agttgagact gctaagacag ttgaagctgt taagttggca gaaaaccttg gtactgtagc
     8701 tatccgttca gcatatgttg ctggtttagc taaagatact gatcaagcaa cagctgctct
     8761 taacgaagcg aaacaagctg ctattgaagc tcttaaacaa gctgcggcag aaacacttgc
     8821 taagattaca actgatgcta aattgactga agctcaaaaa gctgaacaat cagaaaatgt
     8881 atcattagcg cttaagacgg ctattgcaac tgttcgttca gcacaatcta ttgcgtctgt
     8941 gaaagaagca aaagataaag gtattactgc tatccgtgca gcctatgtgc ctaataaggc
     9001 agtcgcaaaa tcatcgtcag cgaaccatct tccaaaatca ggtgatgcaa actcaattgt
     9061 tcttgttggc ttaggagtta tgtctcttct tttaggtatg gtgctttata gcaagaaaaa
     9121 agaaagtaaa gactaagaac ctggctttat ccaaattgct ttctactata ttttcaaacc
     9181 aaggtgattt tgaaatcacc ttggtttttt ggatcgtaag ggcccaagaa cggagtgtat
     9241 tgaaaacatt tcaaagttca attgaaaagg caacagtttt ctgtacagaa tttataaggc
     9301 gttttttgtt caaacgatga ctcatttagt tataggtgtt tgataattaa gattactgtg
     9361 aaggtgatag tagttccacc agtggacttc ctccttggtt ttaagggtta attgttctag
     9421 tgaataaaag gtttcttggt tgcgcagtag ggatcaagcg agcagataaa atacctattt
     9481 taaatcagtt cgagcgaatt ttcgtgctac aagctcaaaa gaggctacta aagtattaga
     9541 atagtttatc tctgagtgaa gttcttgtta taagaagatg atgttatcgt ttgaaacgac
     9601 caaaaaatct cctaactttt actaatttct acatgaaatc tagtccagca tttactctac
     9661 aaccttgatt gattcgctta ataaagacat ttctacttga ttgtttacac atagttatta
     9721 atagaatcta ttataaaatt cagtatatta gatcataatt gtttatatat taatcaatta
     9781 ttattttttt tgtcattttt gataatatta aaaagaaagg gtttacatat taatcatttt
     9841 ttactataat aaaagtgata agaactagat agttgttgtg ttacaacagt acaattgagc
     9901 tagccttgtc cttgttgtgt taactttatt tttaaaataa ggttaaaaat aaacgactcg
     9961 tgttcttatc agttacttat tagataagga ggtaaacctt atgttaaaat ttacttcaaa
    10021 tattttagct actagtgtag ctgaaacaac tcaagttgct cctggaggct gctgttgctg
    10081 ctgtactact tgttgcttct caattgctac tggaagtggt aattctcaag gtggtagcgg
    10141 aagttatacg ccaggtaaat aatctattta gcatctctat gtgatagtga tattaaggta
    10201 atgagtttgc tagcaactaa gttatctttg gtaacttctg attatagatg aatggcatag
    10261 aggtgttaga aaacatgaga caaaagtaat cttcttgatg ctaacgtctc aggtaattag
    10321 caggtactag acagtacctg ctaattacta tatgtttagt aaaatgagat aggaaagata
    10381 gttatgtcat tttttacaaa ggaacaacaa cctaaagaga attgtccgcc aataactgtt
    10441 gaaaaagcaa ggcaattgtt tgaatttaat acaaaccact tgtctttatc ggattaccat
    10501 catcaaacgg tgctaaaaac gtcaaagcag ctagttgctc aacatttaat gcctaatgca
    10561 accgataatc ttagtcaaca ttttttgatg aactataaag ctaataataa ttatttaggc
    10621 ttccaagcta gtattgtcga cttttttaca gattctgccg ttgctaattt ttcaagtagt
    10681 tacgtttatg aaagtcagga aaagataatt cgtttaccaa aacctaccaa gatatcaact
    10741 gctctgtcga catgtattat aaaacgaaga agtcatcgtc aattttcaga tagacaaatg
    10801 cctcttcaag atttatcaaa cattctttat tatgcatgtg gtgttagttc acaagcatca
    10861 attagagatg gagcatcaga taagattaca ctcagaaact gtgcttcagg tggaggttta
    10921 taccctattc atttagtttt ttatgctaga aacatcagta aattaataga tggtttctat
    10981 gaatatctac cctatcagca tgcactaagg tgttatcggc atagctctga ggaaaacgtt
    11041 agagattttg cggaatacgg tgctattaat gctgaaaatt gtaatattat tattatttat
    11101 gtctaccatt acatgaaaaa tacacgtaaa tatgggaatc aggcgactgc ctatgctttt
    11161 attgaatcag gagaaatagc ccagaatatt caattgactg caactgcctt aacttatgga
    11221 agtattgata ttggtggtta taataaggaa tatctccaag aattattaga tttagatggg
    11281 ctaggagagc atgtgattca catgacactc gtaggaacta aggagtctca atgaaatatc
    11341 aacttaatag taatgttcgt gtcgtgacat ttcaagatac attttgtttt agaaaaggaa
    11401 tctgggactt taatgaagcc attctagatt tgacacaaga acctcaaaat ttaaagatag
    11461 tttatcaaga aatagtttca cagttggtta agggagtagc cattgatact gaaacttatg
    11521 aaaatacttt agaacctgag acgttcgcta atttaatgga agtcatttca ggcttgtatt
    11581 acaatgatat gctgatgctt gaggatgact acaatcttga agaaaatgtc atgaaaattt
    11641 taatgggtaa tttccgtttt atggcgcaag caggtcatat ggtaagcaat gatcctgttt
    11701 tgtttatcag tgattcgact tatgttaatg aatctgctga gttgctagct gaacaactgc
    11761 atttacaatt gcaagtggct agcgatgact taaaaatgtt aattcagcag acagatgtta
    11821 gttcgcgttt agatgctttg gagcatcgcc gtaacatgaa ttgtttatca gatgccttgt
    11881 gcgattatca aagtattatt atttgtcaag agcgcttgaa tattatgatg ttgcgacact
    11941 tgaatgaagt tagcgtagct atgaaaaaac aattagtgat tggttttgta gatggaccat
    12001 tcttgcacac ttgtactttg aatcctcctc atagtgctga ctttgatagc ttagaaagaa
    12061 gagttttggc acgtttacaa gactcaactc tctatcaaca ttttgctaat caggttttac
    12121 cagcaacaca agatgtgagc caggcctatt taccattgtt aaatgtttta atgaatttag
    12181 tcgttagcga agctttcatt attgctcaaa caggtagttc taaatttgag ggacgtttat
    12241 taagcattta cttaccgacg cttgagattc aggtccaaga tattttgaaa atgtctaatt
    12301 cacagacaca aggagctttg gcaaaattaa agtatgagga tcagcagata tcaacacgtg
    12361 agattgttaa aaaactcctt gacgagtaac ctataaggag aatctattat gttatactat
    12421 tatccttctt ttaatcatat ttttgatgaa ttaaaaagtt taagtggcaa tcgaacaggt
    12481 atcttaaatc aatctcaagt tccagtttgc aaccatcctc acgatgttta cttaaaaagt
    12541 attactggcc aaatgccaga ttatcataaa caatttatag gtgagttgag tcaagttagt
    12601 tatcacatta ttggttacgg cagttactat gaagaagcac ttatcaagta tttaggagaa
    12661 agtattgaac gctatgctac tgtaattgcc ggagacttac tatcagatcg gattgtctat
    12721 gcttcttata atgagttaaa actgttacat aaagtgatgc ctttagaata cttgcaagtg
    12781 tttacccaag aacagatcgc attatcttgt gacttgcaaa tgatgatgtg cgataaaatg
    12841 gtaactgaga acgatgtatt gggctgggtt aaatgtccca tgttctttga ggacgcagag
    12901 atgtatgttc cagctcaaat gctttgtgtt gggtataaaa caaatgagac tgtaggagaa
    12961 agacggataa taccaggatt ttctacggga acagcttctc acaaaacgtt agatgcggct
    13021 atgtgcaaca gtttgattga atacatccag atcgattcaa tgatgcttag ctggtatact
    13081 aagaagcctt gccccaagat tattgttgat gatcctgata ttgaagtgat tttagaagaa
    13141 gctagactgg gaaaagacag tctatacgac attattccta tcgatatgac tgtaggagaa
    13201 gataatcctc tttatacgtt tggaatcatt ctcaaaaata aatatgatga agggccttac
    13261 ctcttgtttg gagtgcaggc tgggttagat cctaaacatg ctttgctaag agggatcatg
    13321 gaagcttcag ctattagcta tagttactac tataatcttt tatatcaaaa agcttccctt
    13381 gcaaatattg aatgcgaaga acctttgttt ttagatttag atagtaatgt tttctattat
    13441 gcacatccca aggaccaaga ccataaatgg aaggcctttg aacctttgat ttcaggagaa
    13501 gttctgttaa gtgatttgga agatcattct ggtaaagata aaaaagaaga tttgaagact
    13561 ttattagctt atgctaagaa agtcagtcct aatgctgtct ttttagatat tacacctcct
    13621 gaagctctag aaaaaggctg gtacgtcacg cgagttctga tgccagaact tcttgaaatg
    13681 tgtataccag catttccttt tgctaatcat ccacgaatga gacagtttgg aggtgtaaca
    13741 aatgcctttg tccatccaat gccttaatct ttgttttttg ttggtaactt tttgtccttc
    13801 aattcctatg caagctattt ttggaaagga agatagtggt tatgcattta atttaattgg
    13861 ttttctaaga gcaactctga tttatgacat tttggctttg gtaagtattt atgttttgtc
    13921 acctcaaata accttgtctc ttgaaagtat tgatagtaag acgttcttta tgggacttgt
    13981 attttgcgtc tttatagtac tgattgaact cgttttctta catggtttac gttgttggca
    14041 aaaaaagcaa tggcttcctg caactttctc gtttgtagga acgacaaatg attggtctaa
    14101 gattggctat cctttattac tagctttatt tgaagagacg atttatcgtt ttttgtggtt
    14161 taatatatta gcttttcaat ggcatttacc aacaattatt gttttaattg taactagttt
    14221 ttgctatgct ttaaatcact tattgatggg gaaatccatt ttttatgcta agttggtaac
    14281 aggcatcatt tatggtagta tttacatgct gactagtcaa ttgtggttgg ttgtgataat
    14341 gcatgtagga ggtaacttgc tagttgagtg tcttagccat cttcaaacaa aaaagaagaa
    14401 ggaggtgaca tgatgctatt ggttttgctg tcgtttttag gtctaactac tctttggttg
    14461 accagttatc gaagacggtg tttggctaga tggcatcttc aatggttaat ggccttatcc
    14521 tatcaagatt ttttagatgc cttactgagt ctgtttcaat ttgtcattat tatacttgtt
    14581 ttattctttt actcagctac tattaatcta ggagaggttc tgaccttttt gacacaaact
    14641 agctggcatt ggcagattct ttgttattta gtgttatatc ttatggccat catagagatg
    14701 acgcttttag ttttaattct tatctttgat gttttgctcc agaaagacag tcgattgctc
    14761 tttaaaaaaa taacttggct gccttttaga caagataaac cagtcttaag tatactttta
    14821 ctaggcatag ttattctagt agacagtctc ttttatttgg gcttgcttat attattagga
    14881 gaacagtcgc taacaagttt agcaacgctt attttaggtt atgggatagt caaggcttgt
    14941 cgctattcag gttggttaaa tgtactatta gctttttgtc ttttcactat agttggacta
    15001 tgggcagtta cggcaacttt attatatggt tggctagtag gtgcgatgat attaacagtg
    15061 acttatttga tgattagttg caaagagtat tagcaatatt aaaaaaagga gtgatatatg
    15121 agttttgtac aattaacaaa tgttgtcaag tcctacaaaa acggtaagaa agctgtcaat
    15181 gatgtttcct tgtctattga agcaggtaat atttatggtt tgttaggacc aaatggtgca
    15241 ggtaagtcta ccttgattaa tcttatctta ggcttgatcc ctttgagttc cggtaaaatt
    15301 actgttttag ggcaatccca aaagactatt cgaaaaataa gttcgcagat aggttatgtt
    15361 cctcaagaca ttgctgttta tccagaccta actgcttatg aaaatgtagg actatttggg
    15421 tcactttatg gcttaaaggg agctcagctt aaaaaacaag ttctaaaaag tttagaattt
    15481 gtggggctac actcccaagc taagcagttt ccaagtcaat tctcaggagg aatgaagaga
    15541 cggttaaata tcgcttgtgc gctagttcat tcacctaaat taatcatttt tgacgaaccg
    15601 actgtaggga ttgatcctca atcacgtaat catattttag agtcgattcg tttgctaaac
    15661 aaagaaggtg ccacagttat ttatacgacc cattatatgg aagaagtaga ggctctttgt
    15721 gattatattt ttattatgga tcatggtcaa gttattgaag aaggacctaa atttgaactg
    15781 gaaaaacgtt acgttgcaaa tctagcaaac cagattattg taactctaac agattcacgt
    15841 catttggaac tggcagataa gcctgattgg tctttgatag aagatggaga aaaactcatg
    15901 ttgaagattg ataatagtga tatgacatca gttgttcatc agctcacaca ggccaatatt
    15961 acttttagcg agattagaca taaccatttg aatttagaag aaattttctt acacttaaca
    16021 ggtaagaagt tacgagatta gaggttacta tggttttatt tcatttaatc aaaaaagaaa
    16081 gtttacagat ttttagaaac cgaacagcct tattgatgat ggtgattttt ccaattttga
    16141 tgatcgttat tttaagtttt gcctttaaat caagttttaa tactgcgaca acagtcccta
    16201 aattgaccat tcgttaccaa ttagagggtg aaaaaacgga ttaccagaaa aattttcttg
    16261 cttttttaaa agttttaaac caaaaacttc atttagaggc taaacctagt aattctcttg
    16321 aaaaagatcg acaaagagtc agtgaaggag ccttaacggc tgttttagaa gtgaagaaga
    16381 atcagaccat taaggttatt actaataata ttaatcagca aaatgcagat ttgatcaata
    16441 tgctagtaaa aaattatgta gataatgcta aaacttatga ctcgatagca gctctttatc
    16501 ctcaacaatt aaatcatatc agaaagcgaa gtgtggacta tgttaaggtc agttcaatac
    16561 agacaagtaa aggaatgaca tcagctgatt attatgctat ttccatgttt accatgatta
    16621 ctttttatag tatgatgtct gcgatgaacc ttgttttgtc agatcgtcaa caacggatta
    16681 caaatcgtat tcacttaaca ggagtttctc caagtctttt ggtctttgga aaattaatag
    16741 gtgctatgtt agcaacaact gttcaattga gtcttttata catttttaca aggtttgttt
    16801 tacgagttaa ttggggcact aatgagtgga tgcttattgg tataacagcc tccttagtct
    16861 atctctctgt agctgtaggt atcgggctag gcataagcat taaaaatgag gcttttttaa
    16921 cggttgtatc caatactatt attcctatat tcgccttttt aggaggcagt tacgttccat
    16981 tgacaacatt acacagctct attattaatc aattgtcgaa tatatctcct attaaatggg
    17041 ttaatgatag tttgttctat cttatttttg gtggtcaata taatccgatt cctgtaactt
    17101 tgatcgttaa tattagtatc ggaacaattt ttataatatt ggcattgata ggtatgagaa
    17161 agcaggtgac gacatgattt gttttataaa aactctattt gttaagataa agcgcaaaaa
    17221 aacaagttat gtgacattct ttttaatgcc tattttaacg acacttttgg ctttatcttt
    17281 gagtttttca aataataatc aggctaaaat tgggatatta gataaagata atagtcagat
    17341 atctaaacaa tttattgctc aactaaagca aaacaaaaag tacgacattt ttaccaaaat
    17401 caaaaaagag catattgatc actaccttca agataaatct ctagaagcgg tattgactat
    17461 tgacaaagga ttctcagata aagtgttgca aggaaaatct caaaaactca atatacgttc
    17521 gattgctaat agcgaaatta cagagtgggt aaaggcacag accaattatc tattggaaaa
    17581 ttataatatt ataggggatg tggctctagg taacgaagat acttttaata gaattctcca
    17641 aaaaaaccaa cagttaaatt atgatgttaa gcaagtaact ttgacagacc gatcacgtag
    17701 caaagcagta tcttccacaa cgacaggatt tttgcttatt ctaatgttag gtagtacgag
    17761 cgtgatttac agtggtattt tggcagataa atctagtcag ttataccatc gattaatgtt
    17821 atcgaactta tctcgttttc gctatatgtt gagttacgtc tgtgtaggat ttgttgcttt
    17881 tacgattcaa attgtcatta tgctgagttt gttaaaagta tttaatatta gtttttttgt
    17941 tccgacatca cttcttttga ttattttttt cctttttagt ttattagcga taggctttgg
    18001 cctgctgatt ggagctatca cacaaaattc ccaacaaagt agccagttag ctaatcttat
    18061 tgtaatgccg acttcaatgt tggctggctg tttatggcca ttgtctatta cgccttctta
    18121 tatgcaagca attggtaagt tattaccaca aaattgggtt ttatcagcca tagctatttt
    18181 tcaaagtgga gggaccttat ctcaggcttg gccatacttg ttagctctaa tgggaacagc
    18241 actagctttg attagttttt caagtttatt attaaaacct accagactat aattagtctt
    18301 ttgttaaaaa atactttcct aattagctaa ctaccaggta gcccttttat tggcttatat
    18361 gcaataaaag ggttgctttt tgcttatatt ttctctagtg cacttatctt ttgttaaaag
    18421 cgattgcgat gttactgttg ttactgtgtt ttttagagag catattatca aaagcgaccc
    18481 ctctataaag ttcgtttttg ttgataaatg agttgtcaaa gtattttgaa aaacgttttc
    18541 aataaacata aaaactaatg ttattaaaaa aagttttgaa aacgtattta ttttatgaaa
    18601 tatttttgtt acaataagca tgtatcgaat gctataaagc tgtcttgttc gctatcaggg
    18661 gtgcttgtga tgttatgtca gatcaaacaa gtctttaatt gggtgactta acagattaaa
    18721 agaaaagatc taatcgtgtt acttgatagt ataattaatg caaacagaaa gtgtatccca
    18781 attcatagtc tcaggtggag gttaatattc aaaaataaaa tcttgttgtt gtcttaaact
    18841 aatcacatta aaaggagata tcatgattaa caagaaatgt ataatatctg tttcattgtt
    18901 gacactagct attacgctta ctagtgttga agaagttact tcacgccaaa atttgactta
    18961 tgccaatgaa atcgtaacac aaaggccaaa gagagaatct gttattagtg ataaatcgaa
    19021 ttttcccgtc atatcacctt acctagcaag tgtggatttt ggtgagagaa aaacaccttt
    19081 gccaacacct gataaaggag taaaagtaac tactgaacag tctattgctc aagtaagaaa
    19141 ggggcctgaa gaaagaacct atactgttac tggcaagatt acgagtgtga tcaatggctg
    19201 gggaggctat ggcttttata ttcaagatag tgaaggtatt ggactttatg tttatcctca
    19261 aaaagattta ggatacagta agggagatat tgttcaatta acaggtacac ttactcgctt
    19321 taaaggtgat ttacaactcc aacaggtgac tgcacacaaa aagttagagt tatcttttcc
    19381 gacttctgtt aaagaagcag taatatcaga attagaaaca acaacaccct caacattagt
    19441 taagttatct cacgtgacag ttggagaatt atcaactgat caatataaca acacatcttt
    19501 ccttgtaaga gatgatagtg gtaaaagtat agttgttcat atagatcatc gtacaggggt
    19561 taaaggggct gatgttgtta ctaaaataag tcagggtgat ttgattaacc tcacagccat
    19621 attgtctatt gttgatggtc aattacaatt aagaccgttt tctcttgaac aattggaagt
    19681 ggttaaaaag gtcacaagct caaatagtga tgcttcatct cgtaatattg tgaaaatagg
    19741 cgagattcaa ggagctagtc atacgtcgcc acttctcaaa aaagcggtca ccgtagaaca
    19801 ggttgttgtc acttatttag acgattccac tcatttttat gttcaagatc ttaatggtga
    19861 tggtgattta gcgacttcag atggtattcg tgtttttgct aaaaacgcta aggttcaagt
    19921 cggcgatgtt ttgaccattt caggtgaagt ggaagaattc tttggtcgtg gttatgagga
    19981 acgtaagcag actgacctta ccatcaccca aattgtggct aaagcagtga ccaaaacagg
    20041 gacagctcaa gttccatcac cgcttgtttt agggaaagat cgtatcgcgc cagccaatat
    20101 tattgataat gatggcttgc gtgtgtttga tccagaagaa gacgctattg attattggga
    20161 atcaatggaa ggcatgttag tggcggttga tgatgctaaa atccttggtc caatgaaaaa
    20221 taaagaaatt tatgtcttac ctggctctag tacaagaccg ttaaataatt caggtggagt
    20281 attacttcca gctaattctt ataacacaga tgtgattcct gttcttttca aaaaaggcaa
    20341 acaaattatt aaagcaggag attcttacaa aggaagatta gctgggccag tatcttatag
    20401 ctatggtaat tacaaggtct ttgttgatga cagcaaaaac atgccaagtt taatggatgg
    20461 tcatctaaaa cctgaaaaaa caaacttgca aaaagacctt agcaagttaa gcattgcttc
    20521 ttacaatatt gaaaacttct cagccaatcc ttcttcaact aaagatgaga aggtcaaacg
    20581 gattgccgaa tcctttattc atgatctgaa tgctccagac attattggat taattgaagt
    20641 ccaagataat aatgggccga ctgatgatgg gacaacggat gcgacacaaa gcgcacaacg
    20701 cctcattgat gctattaaaa aactaggtgg cccaacttat cgttatgttg atattgctcc
    20761 agaaaataat gttgacggag gtcaaccagg tggtaatatt cgaacaggat tcctttatca
    20821 accagagcgc gtcagccttt ctgataagcc aaaaggcggt gctcgtgatg ctctaacttg
    20881 ggttaataga gaattaaacc ttagtgttgg tcgaattgat ccaactaacg ccgcttggaa
    20941 agatgttcgt aaatcactag cagcagaatt tatcttccaa ggtcgtaaag tcgttgttgt
    21001 tgcaaatcat ttgaactcta agcgtgggga taatgctctt tatggtcgtg tgcaaccagt
    21061 cacttttaaa tctgagcaaa gacgtcacgt cttggctaat atgctagcac aatttgcgaa
    21121 agaaggggca aaacaccaag ctaatattgt gatgctaggt gactttaatg attttgaatt
    21181 cacaaagacg attcaattaa tcgaagaagg tgacatggtt aacttggtga gccgacatga
    21241 tatttcagat cggtattctt attttcacca aggcaataat cagacccttg ataatatatt
    21301 agtttcacgc catttacttg atcactacga atttgacatg gttcatgtga attccccatt
    21361 tatggaagct cacggacgcg catcagatca tgatccattg ttacttcaat tatcattttc
    21421 taaagaaaat gataaggcag agtcttctaa acaaagtgta aaagctaaaa aaacttcaaa
    21481 aggaaaactg ttgccaaaaa caggagatag tcttgtttat gtgataacac tactaggaac
    21541 ggctagttta ttagtgccta ttttattatt gactaaaggc aaaaaggaat catagcgatt
    21601 tgaagaattg gagttcataa agagtcgtta aaggcttgat atacttacgt tagaccttaa
    21661 gctaaaatgg atgactatat aaaaactaag attgataaca aaagaagata acaataagac
    21721 agctttactt gctaaagact tttgtcaagc taattgtttt atggtacaat gtaaccgttg
    21781 ctttgcaacc cttggtgctt agttcccttt caccaagcat attagaaacg tagatagctt
    21841 ttgagagtta tccagacgtt aaaggagaaa aaatgacaaa attgactgta cacgactatg
    21901 ttcacattgg gctagtagct gccttgtatg ttgtcttaac tattacccca cctttaaatg
    21961 cgatttctta tgggatgtat caatttagaa tatctgaaat gatgaatttc ttagcttttt
    22021 atcatcgcaa gtatattatt gcggtgactc ttggatgtat gattgctaac ttttatagtt
    22081 tcggactgat tgatgtcttt gtaggcggcg gttcaactct tatttttgtg accttaggtg
    22141 tgattttgtt tagtaagtat caaaaagatt atctttttaa tggtattttt aataaagctt
    22201 ttgtttactt ttctttcttt tttgcaactt ccatgtttac tgtagcaatc gaactttact
    22261 tctttggagc tccattttta ttaacatggt ttacaactgc gttaggagaa ttagtatctc
    22321 tgctcatagg atcacttatt attgataagt tgtctcaaag aatttccttt tagtcatgca
    22381 tcatactcaa aagagttggg tcaataccct tctctttttt atgttgtttg taatgttaaa
    22441 atcgttctaa tagaaatatt taacacttta gctaatagta ccatatggag tggccaacga
    22501 ttacttttga tgttgttatg atatagaaat agctcatttg ttatctttta tgataaaata
    22561 aaagtatgaa aaaacgcata aaagaattaa cagatcttct taaccgttac cgttatgact
    22621 attatactaa agatgcccca tcagtttctg acagtcacta tgataagctt tatcgtgaac
    22681 tggtgacttt agagcagtca tatcccgagt atgtcttgca agatagccca acgcagcagg
    22741 ttggagggac tattttaaag gggtttgaaa aatatcgaca tcagtatccg ctttttagtt
    22801 tgcaggatgc tttttcacgt gaggagctgg atgcttttga taagcgcgtc aaggccgaat
    22861 ttccgaatgc cacctatcta gcagagttaa aaattgatgg tttatctatt tccttgagtt
    22921 atgagaatgg cttcttacag gttggagcaa cacggggtga tggcaatatc ggtgaaaata
    22981 tcacagaaaa tattaaaaaa ataaaggata ttcctcacca attgagtgag cctttaacca
    23041 tcaccgttcg aggagaggct tacatgtctc gtcagtcctt taaagcgatc aatgaagcac
    23101 gccaagaaaa tggggagact gaatttgcta atcctagaaa tgctgccgct gggacactaa
    23161 ggcagctaga tacggcagtg gttgctaagc gtcaactagc cacttttttg tatcaagaag
    23221 ctagtccgac agctagaaat caacaaaatg aggtcttagc agaattagca gatctaggtt
    23281 tttcagtaaa tccgtattat caactcacat cgtctatgga tgagatttgg aattttatca
    23341 aaactattga ggcaaaaaga gatcaattag cttatgacat agatggcgtt gttattaaag
    23401 ttaatagcct agccatgcaa gaagagctag ggtttacggt taaagctcca cgctgggcca
    23461 ttgcttacaa atttccagca gaagaaaaag aggcagaaat cctttcggtt gactggacag
    23521 tgggacgaac aggcgtggtc acacctacag cgaatctaac tcctgtgcaa ctagcgggga
    23581 caaccgtgag tcgtgcaacc ctacataatg tggattatat tgccgaaaaa gatatccgta
    23641 ttggcgatac agtgattgta tataaggcag gggacattat tcctgctgtc ttaaatgtcg
    23701 ttatgagtaa gcggaaccag caagaagtca tgcttattcc taaactatgc ccatcttgtg
    23761 gtagtga
//
