LOCUS       NZ_CP035431            11882 bp    DNA     circular CON 15-OCT-2024
DEFINITION  Streptococcus pyogenes strain emm105 chromosome, complete genome.
ACCESSION   NZ_CP035431
VERSION     NZ_CP035431.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1800116)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1800116)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005161125.1-RS_2024_10_13
            Annotation Date                   :: 10/13/2024 02:15:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,766
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 39 of 77
            Pseudo Genes (incomplete)         :: 46 of 77
            Pseudo Genes (internal stop)      :: 21 of 77
            Pseudo Genes (multiple problems)  :: 21 of 77
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:05:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 389673
            Orig. end                         :: 401555
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035431.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11882
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11882
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11882
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6882](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6882
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(614..627)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     gene            910..1485
                     /locus_tag="ETT68_RS02110"
                     /old_locus_tag="ETT68_02110"
     CDS             910..1485
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="ETT68_RS02110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02110"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_002985750.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADKANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEEYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
     misc_feature    1616..1630
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            1644..2141
                     /gene="ybeY"
                     /locus_tag="ETT68_RS02115"
                     /old_locus_tag="ETT68_02115"
     CDS             1644..2141
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="ETT68_RS02115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02115"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_002985748.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     gene            2122..2529
                     /locus_tag="ETT68_RS02120"
                     /old_locus_tag="ETT68_02120"
     CDS             2122..2529
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="ETT68_RS02120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02120"
                     /product="diacylglycerol kinase family protein"
                     /protein_id="WP_002985746.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     misc_feature    complement(2581..2596)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            2649..3545
                     /gene="era"
                     /locus_tag="ETT68_RS02125"
                     /old_locus_tag="ETT68_02125"
     CDS             2649..3545
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="ETT68_RS02125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02125"
                     /product="GTPase Era"
                     /protein_id="WP_002985743.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     gene            3566..4042
                     /locus_tag="ETT68_RS02130"
                     /old_locus_tag="ETT68_02130"
     CDS             3566..4042
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="ETT68_RS02130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02130"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002985741.1"
                     /transl_table=11
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKTW
                     AIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFIYE
                     LTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     misc_feature    complement(3593..3609)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.42"
                     /tool="antismash"
     gene            complement(4349..4603)
                     /locus_tag="ETT68_RS09180"
                     /old_locus_tag="ETT68_02135"
     CDS             complement(4349..4603)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="ETT68_RS09180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02135"
                     /product="hypothetical protein"
                     /protein_id="WP_011106868.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSEQVYDLLNEIRMRYPHAHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHKSLRSIRDQIQLYLDELYNSNNG"
     gene            5001..5233
                     /locus_tag="ETT68_RS02140"
                     /old_locus_tag="ETT68_02140"
                     /pseudo=""
     CDS             5001..5233
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /locus_tag="ETT68_RS02140"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02140"
                     /product="Blp family class II bacteriocin"
                     /pseudo=""
                     /sec_met_domain="Bacteriocin_IIc (E-value: 7.9e-10,
                     bitscore: 35.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGGWLL"
     misc_feature    5030..5044
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.12"
                     /tool="antismash"
     gene            5248..5430
                     /locus_tag="ETT68_RS02145"
                     /old_locus_tag="ETT68_02145"
     CDS             5248..5430
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /locus_tag="ETT68_RS02145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02145"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_002987564.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.8e-07,
                     bitscore: 27.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITGG
                     AAAFVCVASGIAAYQ"
     gene            complement(5650..6336)
                     /locus_tag="ETT68_RS02150"
                     /old_locus_tag="ETT68_02150"
                     /pseudo=""
     CDS             complement(5650..6336)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /locus_tag="ETT68_RS02150"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02150"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTQFTTELLNFLAQKQDIDEFFRSSLEIAMNDLLQVELSAFLGYE
                     PYEKEGYNTGNSRNGTYSRQFETKYGLVNLIIPRDRNGEFSPVLLPSYARREDHLEEIV
                     IKLYQTGVTTREISDIIKRMYGHHLFLSGSLVTKRL"
     gene            6655..6882
                     /locus_tag="ETT68_RS02155"
                     /old_locus_tag="ETT68_02155"
     CDS             6655..6882
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="ETT68_RS02155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02155"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_002992018.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 7.2e-17,
                     bitscore: 58.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAIG
                     FPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            6895..7095
                     /locus_tag="ETT68_RS02160"
                     /old_locus_tag="ETT68_02160"
     CDS             6895..7095
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="ETT68_RS02160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02160"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_002985729.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LAGGCVVGFTVGGAYLGYTAN"
     gene            7457..7585
                     /locus_tag="ETT68_RS09185"
                     /old_locus_tag="ETT68_02170"
     CDS             7457..7585
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ETT68_RS09185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ETT68_02170"
                     /product="hypothetical protein"
                     /protein_id="WP_002985724.1"
                     /transl_table=11
                     /translation="MTGMAGAAQGVTVCAQTGVFIPWQGYILCGAAGAATNIIWPH"
     gene            7635..7931
                     /locus_tag="ETT68_RS09340"
                     /old_locus_tag="ETT68_02175"
     CDS             7635..7931
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990766.1"
                     /locus_tag="ETT68_RS09340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02175"
                     /product="hypothetical protein"
                     /protein_id="WP_002985722.1"
                     /transl_table=11
                     /translation="MKILDIIFNLYDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRHP
                     YPIWAAVLGGISLIVIFIYFILIVYLYLRYRYLVWKKIPEIKINSTELSCNHI"
     gene            8110..8520
                     /locus_tag="ETT68_RS02180"
                     /old_locus_tag="ETT68_02180"
     CDS             8110..8520
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985720.1"
                     /locus_tag="ETT68_RS02180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02180"
                     /product="hypothetical protein"
                     /protein_id="WP_002985720.1"
                     /transl_table=11
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVSIYFYLHL
                     TVVCYTIGMILSLGRLISPNHSIFNKLKKRNE"
     gene            8762..9832
                     /locus_tag="ETT68_RS02185"
                     /old_locus_tag="ETT68_02185"
     CDS             8762..9832
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="ETT68_RS02185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02185"
                     /product="hypothetical protein"
                     /protein_id="WP_002985719.1"
                     /transl_table=11
                     /translation="MMMLRDVKVKMSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGVG
                     YKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTVPG
                     MGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGSSGAFPDNIKVSIN
                     PRGGQATIIYGDGSTDIIPSAVLWEKGSVKEPTEADQSVGTPTPGIPGKFKRDQSLNEH
                     EAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVGSWLEPA
                     TALPSVEMSAEDRLKS"
     gene            9925..10323
                     /locus_tag="ETT68_RS02190"
                     /old_locus_tag="ETT68_02190"
     CDS             9925..10323
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /locus_tag="ETT68_RS02190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02190"
                     /product="histidine kinase"
                     /protein_id="WP_172452796.1"
                     /transl_table=11
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLED
                     DAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQYQTGIT
                     YGDDTLADLAHHLLHSDGVIPSLNDKSA"
     gene            complement(10671..10940)
                     /gene="stcA"
                     /locus_tag="ETT68_RS02195"
                     /old_locus_tag="ETT68_02195"
     CDS             complement(10671..10940)
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="ETT68_RS02195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02195"
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_136275484.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIDHSK
                     QNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(11462..11704)
                     /locus_tag="ETT68_RS09345"
                     /old_locus_tag="ETT68_02200"
     CDS             complement(11462..11704)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="ETT68_RS09345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT68_02200"
                     /product="hypothetical protein"
                     /protein_id="WP_002985713.1"
                     /transl_table=11
                     /translation="MKQQGHTVMMCLNKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLH
                     LLPKVVPVLIHLVDKANVSWLTVILQLPILLKTIW"
ORIGIN
        1 gccctactgg ttctagtggg tcgtggcatg gtggtcaaca catcagatgt ggtgacagct
       61 ttatctatgg cagaatctca tcagattgac caatttatgg cgctttatga agaagaaatc
      121 attaaagata actatggtaa ggcaatacga gtcaaaacat taggtcaaaa aacctatgtt
      181 gatagtgtca aacgtcatga tgtggttttt ggtgtaggac ctgcagggac agggaaaacc
      241 ttcttagctg ttacgcttgc agtcactgct ctgaaaagag gacaggtaaa gcgtattatt
      301 ttgacgcgtc ctgcggtaga agctggtgaa agcctaggtt ttttaccagg cgatttgaaa
      361 gaaaaggtgg atccttacct cagaccggtt tacgatgccc tttatcacat tttaggcaag
      421 gaacaaacca cacgcctgat ggaacgagat gtgattgaga ttgctccgtt agcttatatg
      481 cggggtcgta ctctggatga cgcttttgtt atcttagatg aggctcaaaa cacgaccatc
      541 atgcaaatga agatgttttt gacccgtctt ggttttaatt ctaaaatgat tgtcaatgga
      601 gatacgagtc agattgatct ccctcgaaat gtcaagtctg gtttgattga tgctactcag
      661 aaattacaag gcattaaaca gattgatttt gtctatttct ctgctaaaga tgttgtccga
      721 catcctgttg tggctgatat tatcaaggct tatgaaacgt cctcagagga gacgaaggac
      781 ttactaaaac ctaaggctag tgacgttgaa gctgattacc agccaggctt gacaaaatac
      841 cctgtgatcg gtcaagagca ctaagacttt tacgatgatg tttaatatgg cctttttaag
      901 gaggattcta tggatgattt aaccaaagct attatggcag ataaggctaa tctctcctat
      961 accgaaagag gtatttttcc tttatatgat gctcctcaaa cagctaggat tattattgtt
     1021 ggccaagctc ctggaatagt agctcaagaa accaaacttt actggaatga tcgtagtggc
     1081 atccgcttac gagactggct aggtgtggac aatgacacct tttaccactc aggattgttt
     1141 ggcattatcc ctatggattt ctattatcct ggtaagggaa aatccggtga cttaccacct
     1201 agagaaggtt ttgcggctaa gtggcacccg ccattaagag cattgatgcc agaagttgaa
     1261 ttgacgattt tggttggtcg ctatgctcag gacttttacc ttggtaacaa ggcttacaaa
     1321 accttaactg aaacagtccg tcactttgag gagtacttgc cagactattt tccacttgtt
     1381 catccttctc caagaaacca attgtggtta gctaagaacc cctggtttga gcaggattta
     1441 ttacctatct tgcaaaaacg tgtggaggca attctaacca aataggccct gatttaccta
     1501 gcattcccat ttttaagggg cttgtggtat aatgggtaaa taacaaatgc tgattgaaag
     1561 gataagggaa attatctgtc agataataat gggtgcccaa cataccaaat gaggtgggca
     1621 gcccttccct aacaattaac cttatgtata tcgagatgat tgacgaaacg ggacaagttt
     1681 cgcaagagat tatggagcaa acacttgatt tgctcaattt tgctgctcaa aagacgggca
     1741 aagaagaaaa agaaatgtct gtgacctttg tgaccaatga aagaagtcat gaactcaact
     1801 tagaataccg agatactgat cgtccaacag atgtgatttc tctagaatat aagccagaaa
     1861 cgcctatttt atttagtcaa gaggatttgg ctgctgatcc tagtttggca gagatgatgg
     1921 cagagtttga tgcttatatt ggcgaattgt ttatttctat tgacaaggcg cgtgagcagt
     1981 cccaagaata tgggcactct tttgagcgtg aaatgggatt tttggctgtt catggattct
     2041 tacatattaa tgggtatgat cactacaccc ttgaagaaga aaaagagatg tttaccttac
     2101 aggaagagat tttgactgct tatggcctta cacgacaata ataccaccaa acggaaatgg
     2161 aaaaacagaa ccattacctc aagcttggag tttgccttga caggggtttt cacagctttt
     2221 aaggaagaac gcaatctcag aagccacctt ttgtctgcct gtttagcttg tgttgctggt
     2281 ttatttttta gcatttcagc catcgaatgg ttatttttac ttctagccat ttttttggtc
     2341 attaccttag aaattgtcaa ttctgctatt gaaaatgtag ttgatttggc tagtgactat
     2401 cacttttcta tgctagctaa aaatgccaag gatatggcag caggtgctgt tttgatgatt
     2461 tctggctatg ctgtcttgac aggacttatt atttttattc ctaaaatttg gaatattttc
     2521 gttcattaaa aagaaaggaa tgggctatcc tatgtgatga tggcatagga gaataaggaa
     2581 aggacaagac ggttaagctt agtgacaagc ttgatctgaa gccctaccgt taaagcccgt
     2641 aattatctat gtttaaatca ggctttgtag ccattcttgg tcgtccaaat gttggcaaat
     2701 ccaccttttt aaatcacgtc atggggcaaa aaattgccat catgagtgac aaggctcaga
     2761 ccacccgtaa taaaattatg ggcatttaca ccacagaaac agaacaaatt gtctttattg
     2821 atacgccagg tattcataaa ccaaagaccg cccttggtga ttttatggtt gaatctgctt
     2881 atagtacttt acgtgaagtt gagacggttc ttttcatggt tcctgccgat gaaaagcgtg
     2941 gtaagggtga cgacatgatt atcgagcggc taaaagcagc taagattccc gttattttag
     3001 tcatcaacaa aattgataag gttcatccag accaactttt ggaacagatt gatgacttcc
     3061 gtagccaaat ggattttaaa gaagtggtcc caatttctgc cctagaaggt aataatgtgc
     3121 caactttgat caagttatta actgataatc ttgaagaagg tttccaatat tttccagaag
     3181 atcagattac tgatcatcca gaacgtttcc ttgtgtcaga aatggttcgt gagaaggtac
     3241 tgcatttgac gcaacaagag gttccacatt ccgtagctgt tgttgtcgaa tccatgaagc
     3301 gagatgaaga gactgataaa gtgcatatcc gtgcaaccat tatggtggag cgcgatagcc
     3361 aaaaaggcat catcattgga aaacaaggag ccatgctcaa aaaaatcgga aaaatggcgc
     3421 gccgtgatat tgaactcatg ttaggtgaca aggtctactt agaaacgtgg gtcaaagtca
     3481 agaaaaactg gcgggataaa aagcttgacc ttgctgactt tggttataat gagaaagaat
     3541 actaacatag taaagcgagc atcatatgcc acaagactac atttcttata tccgctccaa
     3601 agttgggcat gataagatta ttcttaactt tgctggtggg attttgacca atgatgacgg
     3661 caaggtgctg atgcagttgc gtggtgataa gaaaacctgg gctattcctg gtggcactat
     3721 ggaactgggt gagagctctt tagagacttg taaacgtgag ttcttagagg aaaccggaat
     3781 tgaggttgaa gctgttcgct tactgaatgt ttacactcat tttgaggaag tttatcccaa
     3841 cggtgatgct gttcagacta ttgtctttat ctatgaacta acggctgttt ctgatatggc
     3901 tattgataac tttcataatg aggaaacgct caagttacag tttttctctc atgaggagat
     3961 agcagagtta gagagtgtct cagccaaaca tcgcctgatg ttagaggaat attttagtga
     4021 tagctttgct atggggcatt aagaactctc taggacagtt ggagtatttt tggagattgg
     4081 cttgttaaaa aaatttttga aagacgatgt ttcgagagct tatgctctct ttttttgctt
     4141 ccaatttttc tgattaagtc tgaataattt tgttaaatgt taacaggatt ttctttccaa
     4201 ttgatactag cagacatcaa acatttgttt tagagttatg acgaattgcc acctagccat
     4261 gcatgactta ggcaagttga acctaactgt ggcaatagac ttggaagggg gtgacaagaa
     4321 tcatttctta aaagaattga tgaagtgcct atccattatt cgaattgtaa agttcgtcta
     4381 agtaaagttg aatttggtct cgaatggacc ttaatgattt atgacttgtt gtgtcaataa
     4441 caccaaaaac tcgataatga tttttaaata tttcctcgta attaatatga tttccgatta
     4501 gaataatatg agcatgagga taacgcatac gtatttcgtt tagaaggtca tatacttgct
     4561 cagatttaaa aaagtatgat ttatgataaa ataaacctga catttgtatt tataatctaa
     4621 gtttttccca ctaataattt gagtttttag aaagtatctc tcgaggtaat agttaatata
     4681 gatagatatc atatatccta aaagagaacg atgtcttgta agtattttcg aagactataa
     4741 tattaagttc ggagctcgta ttcataaagg attcggagta tgttttgaca tacttgatag
     4801 cttatttgac taatattcaa aaactctact cttgtgttta cacaacatta ttgacagtct
     4861 ctaatatgtt aaaaagttta tttgagatgt ttatagaacc attcacgaca tagaaaaaaa
     4921 tttttataat aatatgctat atttagtttg tcaaagttgc aacaagatga atataacatt
     4981 ttagaaaggt ttttaaagtt atgataaaat ttgcagaaga aatacaaaaa gaagagttgt
     5041 tccatattat tggaggttat agtgctacag attgtaaaaa tcatttaatt ggtggtatta
     5101 cttcaggtgc tattgcaggt ggttggctac tttaggtgtt ggaggagttg caggtgcgtt
     5161 tgcaggtgca catgtaggtg ctattgcggg tggattaact tgcgttggtg gtatgttatt
     5221 taatggaaaa taattttgag gtgaaaaatg gataattttt tagaattaca gtttgaagaa
     5281 ttagtaaata tttctggtgg aaaaggaaat attggatctg ctatcggagg gtgcttaggt
     5341 gggatgctta tagctgcagc aggaggtcca ataactggag gtgcggcagc atttgtatgt
     5401 gtagcatctg gaattgcagc ttatcaatga tatgattata gttataatac tcggtttaat
     5461 ttgtctaata ctttcccaaa tttttttgaa ggataaagag gtaagagaaa atgcaagtaa
     5521 agtaatttta attgtgatat ttgtttatgt cttactaagt actattacaa attaataata
     5581 ttaaagggat atagattaat tgagactgtc aataatgttg tgtaaacaca agagtagagt
     5641 ttttgaatat tagtcaaata agctatcaag tatgtcaaaa catactccga atcctttatg
     5701 aatacgagct ccgaacttaa tattatagtc ttcgaaaata cttacaagac atcgttctaa
     5761 ggcttcttca tttggaaaaa ccaccctcct tcttgctttg tcgcttgatt tctttattga
     5821 gtgattcaat caagtttgta gagtaaatac tagaccaaat gtaatgtgga aattgataga
     5881 aagttaggag attttccgtt gtttcaagtg atgtcataac ctttttgtaa cgaggcttcc
     5941 actcagaaac aagtggtgac catacatgcg cttaataatg tcactgattt cgcgtgtcgt
     6001 aacgccagtc tgatagagtt tgatcactat ctcttccaag tggtcctctc gtctagcata
     6061 agatggtaat aaaactggtg aaaactcgcc gtttcgatct cttgggatga ttaaattgac
     6121 taagccatac tttgtttcaa actgacgaga ataagtccca ttacggctat tacctgtgtt
     6181 gtaaccttct ttttcataag gttcataacc aagaaaagct gatagttcaa cttgcaagag
     6241 gtcattcata gctatttcta aagaggagcg aaaaaattca tcaatatctt gtttttgtgc
     6301 taggaagtta agtaattctg tggtaaactg agtcatggga ataaatctct ttctagtgaa
     6361 gtgtcgtaac tctactatac aagatttatt cctttttgtg tgtttacaca agatatttta
     6421 cactaccatc aaaaatctta aaaggtattt ataaaagcat gaatggtatc atttaaactt
     6481 cttaattaat taactattgt cacagcgtaa gtgtagagag gataaaaaga catttcatga
     6541 tgaaaaatag ccttttaaga ttttgaaacc atgatactgt tctttatgat attctgtact
     6601 agctcgaggt tgcaacgaga caaatttaag aattatgaaa agaagttatt cgatatggag
     6661 ataaagaaac tggaaacatt tcaccaaatg actatagaaa agcttgccaa ggttgagggt
     6721 ggtaagaata attggcaggc aaatgtcagt ggggttatcg ctgcaggtag tgctggagct
     6781 gctataggtt ttccagtttg cggtgtagcc tgtggttata ttggagctaa aacagctatt
     6841 actctttggg ctggtgtaac aggagctaca ggcggcttct agaggaaaga aaggatgaca
     6901 acaatgaagg aattaacaat taatgacatg gctagtataa gtggaggaaa cgctccagga
     6961 gatgctgtaa ttggtggtct tggaggctta gcttctggat tgaaattttg taaactgcca
     7021 catccagtat tggcaggtgg atgtgttgta ggatttacag ttggtggagc atatttggga
     7081 tatacagcaa attaaatgat ctatacgatt tttattatta tgaatgcgtc tataatgttt
     7141 ttaatcattg ttgatgccat tcataaaaaa aatgttgata aatctatacc atatttagtg
     7201 tttttaattt tcaatcaatt atccctttcg gaaaaatcgg taaacatgtt atctatcgta
     7261 gctttggtag cgtcattatt tgtttttatc gataaaagaa aatagtgatt atgccaatga
     7321 atttagatat cactcttagc tttttaaaat acaaaagagt gaaattagaa tgaaaaagac
     7381 ttcaaaaata tggaagtgct aaaaaaatgg tctgactatc ataaatggag gcggaaaaga
     7441 cggagcaaat atctttttga cagggatggc aggtgctgcg cagggagtga cagtttgtgc
     7501 tcagacaggc gtatttatcc cttggcaagg ttatattttg tgtggagcag ctggtgctgc
     7561 tacaaatatt atctggcctc actaaatata taaatgacta tctatcactg atatataagt
     7621 agtagaggag tggtatgaaa attttagata ttatttttaa cctatatgat agaaagatga
     7681 tatttctaga aaatgttgaa aagctgggag gttacattgt cccttttacc cttatcatct
     7741 tgagtgtagc ctacaacttt aggcaccctt atcctatttg ggctgctgta ctaggaggta
     7801 tatcccttat tgtgatcttt atttatttta tacttattgt ttatctatat ttgcgttata
     7861 gatacttggt gtggaaaaaa ataccagaaa ttaagattaa ttctactgag ctatcatgca
     7921 atcacattta aggggaaaat ggattactta tacaatatca gtacttatga tatttaactt
     7981 tagagactaa agagttaaaa gcaaaatgcg tattgaaaat tggttaaaaa agttataact
     8041 gtctctgata acaggaataa taatttgttc ggttatattt gctaccgata gagtagagga
     8101 ggtgaagata tgcgtaatat attaagattt ttaggtattg ttattatttc tgcagtgatt
     8161 ctatttagta taggtagttt ctacgattta ccattaatga aaaatataca acttatctgt
     8221 tggagtttta tccttgattt attcgttttt gtctacaaac aaagacaaac gacagaagtg
     8281 ctcacatggt atcaagttgt taagcaattt tggttattta ttaagtgtac aattcttatt
     8341 cctaccttaa ttgcttttat tataataata ggatgtttga cgagtatatc agaggtatca
     8401 atttactttt atttacacct tactgttgtt tgctatacaa taggaatgat tttatcatta
     8461 ggtcgtctta tttctcctaa tcactctata tttaataaac ttaaaaagcg aaatgaataa
     8521 tatttaaagt ttgtttttaa ataatgggac tagtttagtg taaccgattc aattaaagaa
     8581 tagttcgtga caagaagata cctttgaagt ttcagaaaca atctgttttc catcctgcta
     8641 tactcatctt tgccaaagct cgtcatttta tatgatttat atagaattac gggttttttg
     8701 ttagactaat atgagaaaat atagtatgac gaattcctaa ataaaaaata atgaaaggaa
     8761 catgatgatg ttaagagatg ttaaagttaa aatgagcagt ttattagttg gttgtgcggc
     8821 tactttacta gtcagtttga gtagtacagt tgcggcagat tcagtccatt cgtctgatag
     8881 acgttttgga gttggctata aggaagaaac aacctctcag ttttcttcgg atcagccctc
     8941 ccaagtggtt ttgtatcgag gtgaggctaa cacggttagg tttacctaca ccaatcagat
     9001 gtatctgatg aaagatattc gcattgcttt ggatggttct ggtaagtctt tgaccgctca
     9061 gactgttcct ggtatgggtc atgtttatga gggctttcaa acttctgcta gagggatttt
     9121 tacgatgtca ggagttcctg aaagcactgt tcccgttgct aaccctaatg tacaaaccaa
     9181 atatacaagg tatttcaaag tcattgatga tatgcataac acaatgtata aaggaactgt
     9241 ttttcttgtt caaccgcaag cttggaaata caccatgaaa tctgttgatc agttaccagt
     9301 agatgacttg aaccatattg gcgttgctgg tattgaacga atgacaactc tcattaaaaa
     9361 tgcgggtgcc cttttaacca caggaagtag tggggctttc ccagacaata ttaaggtatc
     9421 tattaatcca agaggtggtc aggccacgat tatttacgga gatggctcta cggatatcat
     9481 tccatcagcg gtgttatggg aaaaaggctc cgtaaaagag cctactgaag ccgatcaatc
     9541 tgtcggaaca ccgactcctg gtattcctgg taaattcaaa cgagaccaga gccttaacga
     9601 gcatgaagct atggtaaatg tcgaaccact gtctcatgta gtaaaagaca atataaaggt
     9661 catagatgaa aaatcaacag ggcggtttga gccttttaga cctaatgaag atgagaagga
     9721 gaagcctgcc agcgatgtta aggtaagacc agcagaagtt ggtagctggc tagaaccagc
     9781 gacagctctt cctagtgttg aaatgagcgc tgaggacagg ttaaaaagtt agttaataat
     9841 ttgccggttg ctttagacag gcaaacagga aagggcttgg aggtttcttc tgaagtagct
     9901 gtgagcgcct cacagccgga agaattgcat cgattgccta accgtttgat acctgaggaa
     9961 gacaataata aactatcacg agtagccaag gaagtcgtta ctgagccaag gctaaataac
    10021 ccctttttgt tacccaagaa ggttctttcg ttagaagatg atgctgctat ccttaatcat
    10081 ttggaggaag accgtaatgt caataagttg ttcctcgagc cagttgcggc tgaagccaag
    10141 agagaaatta attttcatcc aagtgaccag caggtgactg ctaaagttaa ggagtcatct
    10201 gattttcatc agaaacagta ccaaacaggg attacttatg gagatgatac tctggcagat
    10261 ctggcgcatc atttgcttca ttcagatggt gttattccta gtctaaatga taagagtgct
    10321 taagaattat gtgctagtaa gtctcgctaa gaagaggagt gttacaagat aaataaagaa
    10381 gaggtcaggg aactaccggc ctctttttgt ccgaaatagc gagaatgact ctagcttacc
    10441 tgtatgtagg tccgtgctac acatcttgtt aatgataggg agctgggatc agaaaataat
    10501 tggtatatcg ttttctccta actttttaag aaagtgcggg caaagtgggc tgttagggtg
    10561 atggggttgc tctttcatga tcagtttttt agcttgaaga aagcgtggtg atgagattag
    10621 cttaaaaaag aacactaccg aaatggtagt gtccactgat tttaggagag ctaagattgg
    10681 tcatctaaca ggagtctaac tgtcatgcta ataacagatt tgatagcgct gtccccactt
    10741 ggaagaaata gagccttatt tttatttttt ttgtgtttaa gggcttcttt tttgtgaggg
    10801 ttttgttttg agtggtctat ggtcatcgca gtccttttcc tttcaatagt gtgtttagga
    10861 attggtggtt tagggctgac ggcaagaata aaacacagta tgctaatagc taagataaga
    10921 taaaactgtt ttttggtcat tgtttaggcc tttctagttg aaaaatgtag ttgttgttag
    10981 tttagccttt ttggtagctg gtatttaaaa tagtattagc caagtggttg tactggctat
    11041 ctaaacggta ctcagtattc ctcacgtctt ttccagcaag gaaatacctt gtggtgtatg
    11101 ctgaatcatt ccaggtagca tcaacgtagg aattaacacc atgaatggta acgatgtttc
    11161 aagcatggtg ctcattcaat ccttttttat cacataccaa gctgataagc ctgcagcttc
    11221 tgccatatct ttaaacacga cggcataagc ttgacaaaca ccacacttgc cacgataaag
    11281 agctttagga gtatgattgt tgatgctgct tacggttttg cgattgatgc taagttattt
    11341 tgtggcgtaa gtgtattcgt tgattaaatg gttataaata accttagcct tgtcgtagtc
    11401 actggtgatc ggtttatctg taatattttt ttgacaaagg ttttatattc cttatgaagg
    11461 gctaccaaat tgtctttaag agtataggta actgtaatat cactgttaac catgatacgt
    11521 tggctttgtc taccaagtgt ataagtactg gtacgacttt gggaagtaaa tgaagtaagg
    11581 gttccataat aatcacctca ttcgtcgaga gtggcaccag gtcgttgctg gttaatggag
    11641 ctgatagttt gatagtaagt gttttgttta gacacatcat aacggtatgc ccctgctgtt
    11701 tcatcagctt tgaccttgct gtcaatggca gcaaacgcac ttaagagtaa agctgccgct
    11761 aggtcgctaa cactcttttt aatatctatt agttttcctg ttacaaacta aatataaggg
    11821 tttcccaaaa gtggattgtt tttaagcgaa aggttatcca ccaacgataa tcaggatatc
    11881 ca
//
