LOCUS       NZ_SWMS01000032        10393 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Prauserella endophytica strain CGMCC 4.7182 Scaffold32, whole genome
            shotgun sequence.
ACCESSION   NZ_SWMS01000032
VERSION     NZ_SWMS01000032.1
KEYWORDS    WGS; RefSeq.
SOURCE      Prauserella endophytica
  ORGANISM  Prauserella endophytica
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Prauserella; Prauserella
            coralliicola group.
REFERENCE   1  (bases 1 to 65883)
  AUTHORS   Liu,J.M., Habden,X., Guo,L., Tuo,L., Jiang,Z.K., Liu,S.W., Liu,X.F.,
            Chen,L., Li,R.F., Zhang,Y.Q. and Sun,C.H.
  TITLE     Prauserella endophytica sp. nov., an endophytic actinobacterium
            isolated from Tamarix taklamakanensis
  JOURNAL   Antonie Van Leeuwenhoek 107 (6), 1401-1409 (2015)
   PUBMED   25832628
REFERENCE   2  (bases 1 to 65883)
  AUTHORS   Li,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2019) Yunnan Institute of Microbiology, Yunnan
            University, No. 2 Cuihu North Rd, Kunming, Yunnan 650091, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.16x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005146945.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 16:32:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,852
            CDSs (total)                      :: 7,795
            Genes (coding)                    :: 7,703
            CDSs (with protein)               :: 7,703
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 34 of 92
            Pseudo Genes (incomplete)         :: 69 of 92
            Pseudo Genes (internal stop)      :: 2 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:09:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 47282
            Orig. end                         :: 57675
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SWMS01000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(635..1213)
                     /locus_tag="FCN18_RS34210"
                     /old_locus_tag="FCN18_34205"
     CDS             complement(635..1213)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695049.1"
                     /locus_tag="FCN18_RS34210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34205"
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="WP_137097103.1"
                     /transl_table=11
                     /translation="MPKKLRLTGDAEADKLLSSDPFALLTGLLLDQQFPMEHAFMGPKK
                     IADRMDGFSITKIADTPVEEFVELCVQPPAIHRYGGSMARRVHALANHVLEHYDGKTDR
                     IWKSGKPDGKELLRRVKALPGYGDQKARIFVALLGKQLGVTPEGWREAAGAYGEEGSRR
                     SIADVTSAETLAEVRDFKKAAKAAAKGAG"
     gene            complement(1509..2774)
                     /locus_tag="FCN18_RS34215"
                     /old_locus_tag="FCN18_34210"
     CDS             complement(1509..2774)
                     /GO_function="GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015000.5"
                     /locus_tag="FCN18_RS34215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34210"
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="WP_137097104.1"
                     /transl_table=11
                     /translation="MNRRLRSLGVPLAAAVLATAAVTTITTLGPVDADTTSAASPGKHR
                     QAVVAAHRGSPGLAPEETLASYQRALAEGADVLEGDVQLTADGELVLVHDDTLARTTDV
                     ETVFPERAQGLVGDFTLAEIKRLDAGSWFDPRFAGQRVPTVRELLELNRGRAGLTLELK
                     APQNSPGVGAKLAEELRAAGLADGAILRSGAYRVKVHSRDETALREFHAVAPKVQLAVL
                     TGGKMLDDAMLDALAGWTVGVFAHPRVTTAADIDRAHARGLKVFSDPVDSPAQIEMALH
                     QRYDWVVTNHPETALRVRAGKDAFPGQGAVVVDHVFPNPSGDDVQPETGEHVTLRNTTA
                     RPIDVSGHYVRDAASNLLRIGDGFTIQPGSLLRVYPGPGTNRPDAVYNGLTAGFLNNTG
                     GDTVTLYSPRHRVLDTHSYIVP"
     misc_feature    2814..2827
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.04"
                     /tool="antismash"
     gene            3109..3660
                     /gene="ectA"
                     /locus_tag="FCN18_RS34220"
                     /old_locus_tag="FCN18_34215"
     CDS             3109..3660
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156388.1"
                     /locus_tag="FCN18_RS34220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34215"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_112264612.1"
                     /transl_table=11
                     /translation="MSGKHSIRANGDNQSAGHVVIEKPSRADGAALWRIARDSQKLDLN
                     SSYTYLLWCRDFAETSVVAKVDGNTVGFVIGYRRPDAPDTALVWQVAVDDAQRGRGLAG
                     KLLDALFNRLVADGVRYLDTTITPDNEASIRLFGSFAKRWNAPLERSELFAASDFPDEH
                     EPEDLFRIGPLVRAHDETGR"
     misc_feature    3367..3369
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3714..4967
                     /gene="ectB"
                     /locus_tag="FCN18_RS34225"
                     /old_locus_tag="FCN18_34220"
     CDS             3714..4967
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-357). E-value: 5.4e-72.
                     Score: 234.6. Matches aSDomain:
                     nrpspksdomains_FCN18_RS34225_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806915.1"
                     /locus_tag="FCN18_RS34225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34220"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_112264610.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.9e-76, bitscore:
                     248.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFETLESEVRSYSRGWPTVFDRAQGSWLYAEDGKAYLDFFAGA
                     GALNYGHNNPVLKKALIDYISRDGVTHALDMFTVAKRDFLETLNEVILQPRSMDYKVIF
                     PGPGGANAVEAALKLARKITGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDQYFDGAYPDFLYFERLLEDSGSGLNEPAAVIVETVQGEGGINAARLEWLQGLS
                     ELCKRHNILLILDDVQMGCGRTGPFFSFEEAGIEPDMVCLSKSIGGYGLPLAITLIRPD
                     LDVWSPGEHNGTFRGINPAFVTATEALRTYWRDDELEKSTKAKGERVGAVFTELVENHP
                     EANLVAKGRGLARGLEFPTGEVAGKVCAAAFDRGLLMETSGPDGEVVKLLPALTTTDAE
                     IEQGLEIITDSVKSVLSR"
     aSDomain        3777..4784
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FCN18_RS34225_Aminotran_3.1"
                     /evalue="5.40E-72"
                     /label="FCN18_RS34225_Aminotran_3.1"
                     /locus_tag="FCN18_RS34225"
                     /protein_end="357"
                     /protein_start="21"
                     /score="234.6"
                     /tool="antismash"
                     /translation="FDRAQGSWLYAEDGKAYLDFFAGAGALNYGHNNPVLKKALIDYIS
                     RDGVTHALDMFTVAKRDFLETLNEVILQPRSMDYKVIFPGPGGANAVEAALKLARKITG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDQYFDGAYPDFLYFER
                     LLEDSGSGLNEPAAVIVETVQGEGGINAARLEWLQGLSELCKRHNILLILDDVQMGCGR
                     TGPFFSFEEAGIEPDMVCLSKSIGGYGLPLAITLIRPDLDVWSPGEHNGTFRGINPAFV
                     TATEALRTYWRDDELEKSTKAKGERVGAVFTELVENHPEANLVAKGRGLARGLEF"
     gene            5001..5393
                     /locus_tag="FCN18_RS34230"
                     /old_locus_tag="FCN18_34225"
     CDS             5001..5393
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947387.1"
                     /locus_tag="FCN18_RS34230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34225"
                     /product="ectoine synthase"
                     /protein_id="WP_110343203.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.4e-57, bitscore:
                     185.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLDEVTDTDADIKTPNWRSKRILLAKDKAGFSVHETTLYAG
                     TVNDFWYANHIEAVFVVEGEGEITNKATGETHQLKPGSLYLLNDHDKHQVRPKTEMRTV
                     CVFNPPVTGREVHDENGVYPLVVEED"
     gene            5515..6417
                     /gene="thpD"
                     /locus_tag="FCN18_RS34235"
                     /old_locus_tag="FCN18_34230"
     CDS             5515..6417
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="FCN18_RS34235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34230"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_112264608.1"
                     /sec_met_domain="PhyH (E-value: 2.7e-37, bitscore: 121.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLAESRIEDSYPTRVVGEAQLLERADPTVWGAEGDGPFDAAGLA
                     SHEAKGYTIIEGLLSPAEVQGYWQELVRLSSDSQLKADERVITEKKSGEVRSIFEVHKI
                     SELISELVRDPRILDRARQILGSEVYIHQSRVNYMPGFKGSGFYWHSDFETWHAEDGMP
                     KPRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCVGATPDDHYKASLKEQEIGVPSEDD
                     ITKLAAEYGIDQFTGPAGSALFFDSNIMHGSGNNITPYPRSNIFLVFNSVENALKEPFA
                     ASKPRPTFIGSRDFTPVTR"
     misc_feature    5536..5551
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.79"
                     /tool="antismash"
     gene            complement(6826..7704)
                     /locus_tag="FCN18_RS34245"
                     /old_locus_tag="FCN18_34240"
     CDS             complement(6826..7704)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337642.1"
                     /locus_tag="FCN18_RS34245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34240"
                     /product="hypothetical protein"
                     /protein_id="WP_369973662.1"
                     /transl_table=11
                     /translation="MSVFRGVAENIPGGTWLDDLARASAMAAHAKFERTCRSPLLRVSL
                     LGSSQLDAYTFSDISKALQDATAKIGHIVRNPQQEITYVQPADREKAPLIQRGQAGNAM
                     FFGFPETAAPDALINDGIETLSERAVKELCDFLPANGSDDGALDAVLLQRDTVRNAVSD
                     IVNAVMKTSSLGLSLTPTSGDELMRSMTTEQARVLQSSLRESREDVGYETVTGRLDGVR
                     TRRRIFYLERDTGSTIQGAVAPDLLDEIKLNLDRPVTARLRVVRTTTIDGRRGRPVYEL
                     LEIHSETSLFD"
     gene            complement(7701..8192)
                     /locus_tag="FCN18_RS34250"
                     /old_locus_tag="FCN18_34245"
     CDS             complement(7701..8192)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466521.1"
                     /locus_tag="FCN18_RS34250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34245"
                     /product="DUF6932 family protein"
                     /protein_id="WP_112264606.1"
                     /transl_table=11
                     /translation="MPLPHWTSDNVLPPGRHPADLSDLYERFVWDAPHRNEREVLFSAL
                     SGYLGVVTRLIPSGRAWISGTITVRSEQLPQDVDVVLIPDDWGSLKRLGGPARAALYGQ
                     LTLRGVIAEQPAMYLEQVQPVGGLLDGFLCHPGDEEAWEQTWSRGPADGIVKGFAEVTW
                     "
     gene            complement(8295..8621)
                     /locus_tag="FCN18_RS34255"
                     /old_locus_tag="FCN18_34250"
     CDS             complement(8295..8621)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459640.1"
                     /locus_tag="FCN18_RS34255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34250"
                     /product="hypothetical protein"
                     /protein_id="WP_112264604.1"
                     /transl_table=11
                     /translation="MLWLFGQIWLWLLISFALGAGLTALLFRRPARQRPPERPVPPPAA
                     PPPAEAEETRHLPATGALLPEPSPDHSHQEGTLPPRRAWHVRNEWPDEQDAAASEDQES
                     RRGG"
     gene            complement(8636..9313)
                     /locus_tag="FCN18_RS34260"
                     /old_locus_tag="FCN18_34255"
     CDS             complement(8636..9313)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441523.1"
                     /locus_tag="FCN18_RS34260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34255"
                     /product="OmpA family protein"
                     /protein_id="WP_112264602.1"
                     /transl_table=11
                     /translation="MSGGGGRPWLVPLALLVTGALAFLATRFEADGIESDLLARSRSAL
                     ADAGLPGASVSFEGRDATVSGLSAREAEWAVGVVAGVEGVRAADAAESTPRREPGVPED
                     ARQRLQAELDGLLATQPISFQPDTAVLTPEGESAMTAVLELLGTTPTDIRFEVGGHVAR
                     LPGGDPAGARALSQERADAVAQRLIASGIANTRVTAIGYGDTRPLSPSGDTGADRRVEI
                     TVR"
     gene            complement(9445..10251)
                     /locus_tag="FCN18_RS34265"
                     /old_locus_tag="FCN18_34260"
     CDS             complement(9445..10251)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156380.1"
                     /locus_tag="FCN18_RS34265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FCN18_34260"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_112264600.1"
                     /transl_table=11
                     /translation="MTGYNSYVAIGDSFTEGLNDPLPDGSFRGWADRLAEILADGRSEF
                     AYANLAIRGKLLAEIMSEQLPIALETRPDLVTVCAGGNDIIVPGADVDAVASAFEEGIA
                     ALRAAGIEVVIFTGPDTKRMSVMSILRGKVAIYNAHLWAIAERHGAKVVDLWAMDALHD
                     VRAWSDDRLHFTPEGHRRIALRTAEVLGVPTASDWREPWPETDGHPNWLMLRRSDLEWT
                     RVHLLPWIRRHLRGESMGDGITPKRPRLTPLAERPAGTTLIEPAEN"
     misc_feature    10208..10220
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
ORIGIN
        1 gcttctgcac cggcgcgttc acgctcgccg cggcgggtct gctcgacggg cgcagggcca
       61 ccacgcactg gcggtgggcg gaggtcttcg ccgcccggtt cccgtcggtg cggctggaac
      121 gggacgtgct cttcgtcgac gacggtgacg tgctcacggc ggcgggcagc gccgccgcgc
      181 tcgacctcgg gctgcacgtg gtccggcgcg accacggtgc cgagatcgcg aacgcagtga
      241 gcaggcggct ggtgttcggc gcgcaccggg acggcggtca acagcagttc atcgaacggc
      301 cggtgcccga ggtaccggac gcgtcgctcg caccggtgct cgcctgggct caggcgcggc
      361 tggaccagcc actgacggtc gccgacctcg ccgctcgggc agcgatgagc caggccacgc
      421 tgcaccgccg gttccgcgcc gaactcggca cgacgccgct cggctggctc acggccgaac
      481 gcgtggacct tgcgtgcagg ctcatcgagc gtggtgagca gcgactcgac gtcgtggcct
      541 cccgcagcgg gctgggcagc gtggccaacc tgagggcgcg gctgcgcagg cgaaccgggc
      601 tgagcccgac ggagtaccgc aagcggttcg cgagctagcc tgcgcccttg gccgcggcct
      661 tggccgcctt cttgaagtcg cggacctccg cgagggtctc ggcgctggtg acgtcggcga
      721 tcgaccggcg cgagccctcc tcgccgtagg cgcccgccgc ctcgcgccag ccctccggcg
      781 tcacgcccag ctgcttgccg aggagcgcaa cgaagatcct ggccttctgg tcgccgtagc
      841 ccggcagtgc cttgacccgc cgcagcagtt ccttgccgtc cggcttgccg gacttccaga
      901 tcctgtccgt tttgccgtcg tagtgctcga ggacgtgatt cgccagagcg tggactcgcc
      961 gagccatcga tccgccgtag cggtggatgg caggcggctg cacgcacagc tcgacgaact
     1021 cctcgacagg ggtatcggcg atcttggtga tgctgaagcc gtccatgcgg tccgcgatct
     1081 tcttcggccc catgaacgcg tgctccatcg ggaactgctg atcgaggagc agaccggtga
     1141 gcagcgcgaa ggggtcgctg ctgagcagct tgtcggcctc ggcgtcgccg gtgaggcgca
     1201 gcttcttggg catggctcca tcgtcgcacg tggcgtggac gcggacctat gcgcgcgggc
     1261 acccggcccg gagaggaggc ggctcgacac ggcgaacccg tgccgcgcac accggcacgg
     1321 cactggcgct cgaaaggtcg ccgcgagcgt tcgccggtcc tccaaggctc agcgccggcg
     1381 agggtcggcg gcagcggcca gatgtccgcc catggcggga cggatcacca cggtgtccag
     1441 gtcgtgcgga caggcgtgac ggggcaccgg gccggggtgc gacgtggggc cacgccgcac
     1501 cccaccggtc acgggacgat gtaggagtgg gtgtcgagca cccggtggcg cggcgagtag
     1561 agggtcaccg tgtcgccgcc ggtgttgttc aggaacccgg cggtcagccc gttgtagacg
     1621 gcgtccgggc ggttcgtgcc cggaccgggg tagacccgca gcaggctgcc gggctggatc
     1681 gtaaagccgt cgccgatgcg caacaggttg ctcgccgcgt cgcgcacgta gtgcccgctc
     1741 acgtcgatcg gcctggccgt ggtgttgcgc agggtgacgt gctcgccggt ctccggctgg
     1801 acgtcgtcgc cgctcgggtt cgggaagacg tggtcgacga cgacggcgcc ctgtcccggg
     1861 aacgcgtcct tgcccgcgcg cacgcgcagc gccgtctccg ggtggttggt gaccacccag
     1921 tcgtaccgct ggtgcagggc catctcgatc tgcgcgggac tgtccaccgg gtcgctgaac
     1981 accttgagcc cgcgcgcgtg cgcccggtcg atgtcggccg cggtcgtcac gcgcgggtgg
     2041 gcgaacactc ccaccgtcca gccggcgagc gcgtcgagca tggcgtcgtc gagcatcttg
     2101 ccgccggtca gcaccgccag ctgcaccttg ggcgcgaccg cgtggaactc ccgcagagcg
     2161 gtctcgtcgc gggagtggac cttcacgcgg taggcgccgg agcgcaggat cgcgccgtcg
     2221 gcgagcccgg ccgcccgcag ttcctcggcg agcttcgcac cgacaccggg gctgttctgc
     2281 ggcgctttga gctccagcgt cagccctgcg cggccgcggt tgagctccag cagctcgcgc
     2341 acggtcggca cgcgctggcc cgcgaaacgg gggtcgaacc acgaacccgc gtcgagccgc
     2401 ttgatctcgg cgagggtgaa gtcgccgacc aggccctgcg ctcgctcggg gaacacggtc
     2461 tcgacgtcgg tggtgcgggc cagcgtgtcg tcgtgcacca gcacgagctc gccgtcggcc
     2521 gtgagctgca cgtcgccttc gagcacgtcg gcgccttccg ccagcgcgcg ctggtaggag
     2581 gcgagcgtct cctcgggcgc gaggccgggt gagccgcggt gggcggcgac gaccgcctgg
     2641 cggtgcttgc ccggactcgc agccgaggtg gtgtcggcgt caacgggtcc gagcgtcgtg
     2701 atcgtggtga cggcggcggt ggcgagcacc gcggcggcga gcggcacgcc gaggctgcgc
     2761 aggcgtcggt tcacggcaaa cctttcgatg gggatcggaa ccggttgatc ttcgaaccgg
     2821 tcgtttgcga atccccgact tcgagccgaa cctggccgga acacagggtg acgcagagtc
     2881 gcgctcggct gacccctcgc gtgcgacggc gtgtgccact accgtgctag acgtgagccg
     2941 ctgagagctc agcggccgga cgtgctgcgc ttatagcaca actgacgcca tgatcggtac
     3001 atttctgtcg gcattgtcac atgtgtttct gtgtcgttgc aggtaggcgg agggtccgca
     3061 tcgatctggg gctgagcggc gtaacgcact ctgttacgtt gcttgaatat gtccggaaag
     3121 cactcaatcc gtgccaacgg cgataatcaa tcggcgggcc acgtggtgat cgaaaagcca
     3181 tcccgagctg atggtgccgc cctgtggcga atagcgcgtg attcgcaaaa acttgacctc
     3241 aactcgtcgt acacgtacct gttgtggtgt cgcgatttcg ctgagacttc tgttgtcgcc
     3301 aaggtcgatg gaaatactgt ggggtttgtc atcggctatc gcaggcccga cgcgcccgac
     3361 accgcgttag tgtggcaagt cgcggtcgac gacgcccagc gtggccgcgg cctggcagga
     3421 aagctgctcg acgcgctgtt caaccgcctc gtcgccgacg gagtgcgcta cctggacacc
     3481 acgatcacgc cggacaacga agcgtcgatc cggctgttcg gttccttcgc caagcgatgg
     3541 aacgcccctc tcgagcggtc cgagctgttc gcggcgagtg attttcccga tgagcacgag
     3601 ccggaggacc tgttccgcat cggtccgctc gtgcgagccc acgatgagac tgggcgctag
     3661 aaagcgaaaa cccaacaatt gttttcgccc gataaagcag gagacgaagc aaggtgagca
     3721 tcttcgaaac gctcgaatcc gaggtgcgca gctacagccg tggctggccg accgtgttcg
     3781 atcgagcgca aggcagttgg ctctacgcgg aggacggcaa agcgtacctt gacttcttcg
     3841 ccggagccgg tgcgctcaac tacgggcaca acaacccggt gctgaagaaa gcactgatcg
     3901 actacatttc gcgcgacggc gtgacccacg cgctcgacat gttcaccgtt gccaagcgcg
     3961 acttcctgga gaccctcaac gaggtgatcc tccagccgcg ctcgatggac tacaaggtca
     4021 tcttccccgg ccccgggggc gccaacgcgg tcgaggccgc gctcaagctc gcgcgcaaga
     4081 tcaccggcaa ggaatcggtc atcaacttca ccaacgcctt ccacgggatg accctgggcg
     4141 cgctgtcggt gaccggtaac tcgatgaagc gcggcggcgc gggcatcccg ctggttcacg
     4201 ccacgccgat gccgtacgac cagtacttcg acggcgccta ccccgacttt ctctacttcg
     4261 agcgcctgct cgaggactcc ggcagcggtc tgaacgagcc cgccgccgtg atcgtagaga
     4321 cggtgcaggg cgagggcggc atcaacgccg cgcggctcga gtggctgcag ggcctctccg
     4381 agctctgcaa gcggcacaac atcctgctga tcctcgacga cgtgcagatg gggtgcggtc
     4441 gcaccggccc gttcttcagc ttcgaggagg caggcatcga gccggacatg gtctgcctct
     4501 cgaagtcgat cggcggatac ggccttccgc tcgcgatcac gctcatccgg cccgacctcg
     4561 acgtgtggtc gcctggtgag cacaacggca ccttccgcgg catcaacccg gcgttcgtca
     4621 ccgccaccga ggccctgcgc acctactggc gcgacgacga gctggagaag tccaccaagg
     4681 ccaagggcga gcgggtcggc gccgtgttca ccgagctggt ggagaatcac cccgaggcga
     4741 acctggtcgc gaagggccgc ggcctcgcca ggggcctcga gttcccgacc ggcgaggtgg
     4801 cgggcaaggt ctgcgcggcg gccttcgacc gcggcctgct catggagacg tccggtcccg
     4861 acggcgaggt cgtcaagctg ctgcccgcgc tgaccaccac cgacgccgag atcgagcagg
     4921 gcctggagat catcacggac tccgtgaagt ccgtgctgtc gcgctgagtc agttttcgta
     4981 caggagaaag ggagtacgtt ttgatcgtcc gcacactcga cgaggtcacc gacaccgacg
     5041 ccgacatcaa gacgccgaac tggcggagca agcgcatcct gctcgccaag gacaaggcgg
     5101 gcttctcggt gcacgagacc acgttgtacg cgggtaccgt caacgacttc tggtacgcca
     5161 accacatcga ggccgttttc gtcgtcgagg gcgagggcga gatcacgaac aaggcgaccg
     5221 gcgagaccca tcagctcaag cccggttcgc tttacctgct gaacgaccac gacaagcacc
     5281 aggtccggcc gaagaccgag atgcgcaccg tctgcgtgtt caacccgccg gtcaccgggc
     5341 gcgaggtcca cgacgagaac ggcgtgtacc cgctcgtggt cgaggaagac tgactgactg
     5401 ccgagacgtc gcgataggcg tgccggggtc gagcggcccc ggcacgccgc atcgcgaacc
     5461 ccgagggggc acagaaaaca aacaaaagag tggcactcgt aggaggcgag tattttgact
     5521 ctcgcagaga gccggattga ggacagttat ccaacccgtg tggtcggtga ggcgcagctg
     5581 ctcgaacggg ctgatcccac agtgtggggc gccgagggag acgggccgtt cgacgcggcc
     5641 ggactcgcct cacacgaggc gaagggctac acgatcatcg agggcctgtt gtcgcccgcc
     5701 gaggtgcagg gttactggca ggaactcgta cggctctcgt cggactccca gctcaaggcc
     5761 gacgagcgcg tgatcaccga gaagaagtcc ggcgaggtcc ggtcgatctt cgaggtgcac
     5821 aagatcagcg agctgatcag cgaactggtg cgcgacccgc gcatcctgga ccgggcaagg
     5881 cagatcctgg gctcggaggt ctacatccac cagagccgcg tgaactacat gccggggttc
     5941 aagggctcgg gcttctactg gcactccgac ttcgagacgt ggcacgccga ggacgggatg
     6001 ccgaagccgc gcgcggtgag ttgctcgatc gcgctcaccg acaactaccc cttcaacggt
     6061 ggcctgatgg tgatgcccgg ctcgcagcgc acgttcgtgc agtgcgtcgg cgccacgccg
     6121 gacgaccact acaaggcgtc gctgaaggag caggagatcg gcgtcccgag cgaggatgac
     6181 atcaccaagc tcgccgccga gtacgggatc gaccagttca cgggtcccgc gggctccgcg
     6241 ctgttcttcg actcgaacat catgcacggc tcgggcaaca acatcacccc gtatccgcgc
     6301 tcgaacatct tcctggtgtt caacagcgtc gagaacgcgc tgaaggagcc gttcgcggcc
     6361 agcaagcccc ggccgacctt catcggcagc agggacttca cccccgtcac tcgctgaccc
     6421 gtggcctccg ggtaagcgat tggtaacaag gcgtgccggg gtggttgtag ccgcctcggc
     6481 gcggcctgtt accgtgtcgc acacctcgtc gcgttaccga tgaggtgatc gtggccccag
     6541 tggcgcgggt ggctccttgc tcggggtgag tccgcaccgt cggtcacgaa gcgagtacga
     6601 gtgagtacgg ccccgcgcca ccagatcggg actggtggca cggggccgta ttcatgtccg
     6661 cccgagaccg agacgctcgc gatctctccg ctggtgctcg cccaccggta ccacaccgac
     6721 cgcgtcttca cgctcgacca gcgcgactac cgggcgatga ccccgttgcc gccccggttc
     6781 cgctcgttcc gcatcctgcc cgcggatggc aggggacgag ccgcctcagt cgaagaggct
     6841 cgtctccgag tggatctcca gcagttcgta caccgggcgg ccccgccgtc catcgatcgt
     6901 cgtcgtcctg accacgcgca gccgcgccgt gaccgggcgg tcgaggttga gcttgatctc
     6961 gtcgagcagg tccggcgcga cggcgccctg gatcgtgctg ccggtgtcgc gctcgaggta
     7021 gaagatgcgc cgccgggtcc gcacgccgtc gagcctgccg gtcaccgtct cgtagccgac
     7081 gtcctcgcga gactcgcgca ggctgctctg cagcacgcgc gcctgctcgg tggtcatgct
     7141 gcgcatcagt tcgtcgccgc tcgtcggggt cagcgacagc cctaggctcg acgtcttcat
     7201 caccgcgttg acgatgtcgc tcacggcgtt gcgcacggtg tcgcgctgca gcagcaccgc
     7261 atcgagcgcg ccgtcgtcgg agccgttggc aggcaggaag tcgcacagtt ccttgaccgc
     7321 ccgctccgac agcgtctcga tgccgtcgtt gatgagcgcg tcgggcgcgg ccgtctcagg
     7381 gaaaccgaag aacatcgcgt tgcccgcctg cccgcgctgg atcagcggcg ccttctcgcg
     7441 gtcggcgggc tgcacgtagg tgatctcctg ctgcgggttg cgcacgatgt ggccgatctt
     7501 ggccgtcgcg tcctgcagcg ccttgctgat gtcggagaag gtgtacgcgt cgagctggga
     7561 ggacccgagc agcgacaccc gcagtagcgg cgagcggcac gtgcgctcga acttggcatg
     7621 cgcggccatc gccgacgccc tcgccaggtc gtcgagccag gtgccgccgg gaatgttctc
     7681 ggcgacgcca cggaagacgc tcaccaggtc acctccgcga agcccttgac gatgccgtcg
     7741 gccgggccgc gggaccacgt ctgttcccac gcctcctcgt ccccgggatg gcagaggaac
     7801 ccgtcgagca gaccgccgac cggttgcacc tgctccaggt acatcgcggg ctgctccgcg
     7861 atcacgccgc gcagcgtgag ctgcccgtac agcgccgcgc gggcggggcc gccgagccgc
     7921 ttgaggctgc cccagtcgtc ggggatcagc accacgtcga cgtcctgcgg cagctgctcg
     7981 ctgcgcacgg tgatggtgcc gctgatccag gctcgcccgg acgggatcag ccgcgtcacg
     8041 acgccgaggt agccactgag tgcgctgaag aggacctcgc gctcgttgcg gtgcggggcg
     8101 tcccacacga accgctcgta gaggtcggag agatcggcgg ggtgacggcc gggtggcaac
     8161 acgttgtccg aggtccagtg cgggagcggc atcaacgttc cttacctcgt cctcgaacgc
     8221 acccggagac ccacacccgc ctggcagggg gtgaccgggt gcagcgaatt tatcagcagc
     8281 gggtgagcgg cgggtcagcc gcctcgccgg gactcctggt cctcgctcgc cgccgcgtcc
     8341 tgctcgtcgg gccattcgtt gcggacgtgc caggcccgcc ggggcgggag cgtgccctcc
     8401 tgatggctgt ggtcaggcga gggttccgga agcagcgcgc cggtggcagg cagatgccgg
     8461 gtctcctcgg cctcggccgg tgggggcgcc gcgggaggcg ggacgggacg ctcgggcggg
     8521 cgttgtcgcg cagggcgccg gaacagcagg gcggtgagcc cggcgccgag cgcgaacgag
     8581 atcagcagcc agagccagat ctgcccgaac aaccacagca tcgctctccc ccttctcacc
     8641 gcaccgtgat ctccacccgc cggtcggcgc cggtgtcgcc gctcgggctg agcggacgtg
     8701 tgtcgccgta gccgatggcg gtgaccctcg tgttggcgat gcccgacgcg atcagccgct
     8761 gggccaccgc gtcggcccgc tcctgcgaga gcgcccgcgc gccggccggg tccccgcccg
     8821 gcaggcgggc gacgtgcccg ccgacctcga agcgaatgtc cgtgggcgtg gtgccgagca
     8881 gttcgagcac cgcggtcatc gcgctctcgc cctcaggggt gagcacggcg gtgtccggct
     8941 ggaacgagat cggctgcgtg gccagcaggc cgtccagctc ggcttgcagc cgctgccggg
     9001 catcctcggg taccccgggt tcgcgtcgcg gggtggactc cgccgcatcg gcggcgcgaa
     9061 ccccctccac gcctgccacg acgcccaccg cccactcggc ctcgcgggcg gacagtccgg
     9121 aaacggtcgc gtccctgccc tcgaagctga cggacgcgcc gggcagcccg gcgtcggcga
     9181 gtgcggaccg tgaccgggca agcagatccg actcgatccc atcggcttcg aatcgagttg
     9241 cgaggaaggc gagcgcgccc gtcaccagca gcgcgagcgg aaccagccac ggccggcctc
     9301 caccaccgga catgaaccga ctgtaaacgc gcgctcgcgg aagcgcgaaa cgtgccggct
     9361 cgccgcggcc gcagtgaagg ccaccatcac tgcgttcaac gcaggcatgg tggccttcac
     9421 tgcagttcgg tggccggccg ccgcctagtt ctcagcgggt tcgatcaggg tcgtgccggc
     9481 ggggcgctcg gcgagcgggg tcaggcgcgg gcgctttgga gtgatgccgt cgcccatcga
     9541 ctcgccgcgc aggtgcctgc ggatccacgg cagtaggtgc accctcgtcc actcgaggtc
     9601 cgagcggcgc agcatgagcc agttcgggtg cccgtcggtc tccggccacg gctcacgcca
     9661 gtcgcttgcg gtcggcaccc cgagcacctc ggcggtccgc agggcgatcc gccggtggcc
     9721 ctctggcgtg aagtgcaggc ggtcgtcgct ccacgcgcgg acgtcgtgca gcgcgtccat
     9781 cgcccacagg tcgacgacct tcgcgccgtg ccgctccgcg atggcccaca ggtgtgcgtt
     9841 gtagatggcg accttgcccc gcaggatgct catcaccgac atgcgtttgg tgtcggggcc
     9901 ggtgaagatc acgacctcga tcccggccgc gcgcagcgcc gcgatgccct cctcgaaggc
     9961 cgacgcgacc gcgtcgacgt cggcgccggg cacgatgatg tcgttgccgc ctgcgcagac
    10021 cgtgaccagg tcgggacggg tctcgagcgc gatcggcaac tgctcactca tgatctcggc
    10081 gagcagtttc cccctgatcg cgagattggc gtaggcgaac tcggagcgtc cgtcggcgag
    10141 gatctcggcc aggcggtcgg cccagcctcg gaagctgccg tccggcaagg ggtcgttgag
    10201 cccctcggtg aaactgtctc cgatcgcgac gtagctgttg tacccggtca cgaggtctcc
    10261 ctccgacaac gccctgtgaa ccccttctcg tgtcgatcaa caaccggacg cccgcccgca
    10321 gtcccaccta cggtgtccgg atctttgatc ggacgtcata gtgtcttcct tcgtcggcca
    10381 cctacgcacg cgt
//
