GCF_004799565.1 - 13 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP039297.1 (Bacillus velezensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	lanthipeptide-class-ii	97,330	120,371
Region 2	NRPS	324,128	389,535	High	surfactin	NRPS:Type I
Region 3	PKS-like	966,270	1,007,514
Region 4	terpene	1,090,315	1,111,055
Region 5	transAT-PKS	1,416,078	1,504,311	High	macrolactin H	PKS
Region 6	transAT-PKS,T3PKS,NRPS	1,723,019	1,833,118	High	bacillaene	NRPS:Type I+PKS:Type I
Region 7	NRPS,transAT-PKS,betalactone	1,895,285	2,033,109	High	fengycin	NRPS:Type I
Region 8	terpene	2,057,217	2,079,100
Region 9	T3PKS	2,142,426	2,183,526
Region 10	transAT-PKS	2,298,677	2,404,862	High	difficidin	PKS
Region 11	terpene-precursor	2,428,193	2,449,083
Region 12	terpene-precursor,NRP-metallophore,NRPS,RiPP-like	3,018,096	3,083,411	High	bacillibactin	NRPS:Type I
Region 13	other	3,607,953	3,649,371	High	bacilysin	other:other

NZ_CP039297.1 - Region 1 - lanthipeptide-class-ii

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Location: 97,330 - 120,371 nt. (total: 23,042 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

E4T61_RS00585: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase

E4T61_RS00575: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000510.3	0.54	ribosomal	epilancin K7	Staphylococcus epidermidis
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000500.5	0.50	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000504.5	0.49	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001407.5	0.44	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0001210.5	0.43	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000560.5	0.43	ribosomal	subtilomycin	Bacillus subtilis
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000510.3	0.54	ribosomal	epilancin K7	Staphylococcus epidermidis
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000500.5	0.50	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000504.5	0.49	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001407.5	0.44	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0001210.5	0.43	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000560.5	0.43	ribosomal	subtilomycin	Bacillus subtilis
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for E4T61_RS00575

E4T61_RS00570

Leader:

MSEKQIERTIRAWKDPEFRKTLSDVADHPSGSIEARQLSMLGS

Core:

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

Core with dehydrations:

EENVNPMDhbDhbPVDhaPAPDhbVVIRVDhaLKVCysPDhbFKIKCysKV

antiSMASH-DB 4.0 matches for E4T61_RS00570

100.0%

NZ_CP039297.1and 2 others:

NZ_CP040378.1 Lanthipeptides: D1120_RS00435_lanthipeptideNZ_CP059318.1 Lanthipeptides: HZT45_RS00565_lanthipeptide

Lanthipeptides: E4T61_RS00570_lanthipeptide

1

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

1

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

97.1%

NZ_CP040672.1and 1 other:

NZ_CP087137.1 Lanthipeptides: LOK79_RS00585_lanthipeptide

Lanthipeptides: FGE23_RS05605_lanthipeptide

1

EENVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

E+NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

1

EDNVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCKV

35

42.9%

NC_018499.1and 5 others:

NC_018499.1 Lanthipeptides: BCK_RS27840_lanthipeptideNC_018499.1 Lanthipeptides: BCK_RS27835_lanthipeptideNC_018499.1 Lanthipeptides: BCK_RS27845_lanthipeptideNC_018499.1 Lanthipeptides: BCK_RS27840_lanthipeptideNC_018499.1 Lanthipeptides: BCK_RS27835_lanthipeptide

Lanthipeptides: BCK_RS27845_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCK

34

+VNP TTP +P ++ VSL +CPT K K

2

DVNPETTPATP---TIVAVSLGICPTTKCTSK

30

with 43 more sequence matches

Only the first 10 are displayed here.

42.9%

NZ_CP023481.1and 2 others:

NZ_JALARN010000006.1 Lanthipeptides: MOE03_RS17410_lanthipeptideNZ_JAQCPU010000013.1 Lanthipeptides: PG077_RS20425_lanthipeptide

Lanthipeptides: COP00_RS24795_lanthipeptide

5

NPMTTPVSPAPTVVIRV---SLKVCPTFKIKC

33

N TP S V +RV S+K CP+FK KC

1

NDEITPQSTVVCVSLRVCKWSIKYCPSFKRKC

32

42.9%

NZ_PIJH01000018.1:

Lanthipeptides: CV632_RS18055_lanthipeptide

9

TPVSPAPTVV---IRV---SLKVCPTFKIKC

33

T VSP T+V +R+ SL+ CP+FK+KC

2

TEVSPQSTIVCVSLRICNWSLRFCPSFKVKC

32

40.0%

NC_009328.1:

Lanthipeptides: GTNG_RS18990_lanthipeptide

9

TPVSPAPTVV---IRV---SLKVCPTFKIKC

33

T VSP T+V +R+ SL+ CP+FK++C

2

TEVSPQSTIVCVSLRICNWSLRFCPSFKVRC

32

40.0%

NZ_CP013862.1and 1 other:

NZ_CP013862.1 Lanthipeptides: AOX59_RS09365_lanthipeptide

Lanthipeptides: AOX59_RS09365_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFK

30

+V+P TTP S A I VS CPT K

2

DVDPQTTPSSVA--CGIAVSALFCPTTK

27

40.0%

NZ_CP015591.1and 15 others:

NZ_CP078085.1 Lanthipeptides: KU891_RS29355_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29350_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29345_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29340_lanthipeptideNZ_CP078085.1 Lanthipeptides: KU891_RS29335_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29930_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29925_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29920_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29910_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29930_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29925_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29920_lanthipeptideNZ_CP085411.1 Lanthipeptides: LIT28_RS29910_lanthipeptideNZ_QNUC01000031.1 Lanthipeptides: DN396_RS23050_lanthipeptideNZ_QNUC01000031.1 Lanthipeptides: DN396_RS23050_lanthipeptide

Lanthipeptides: A7A70_RS27550_lanthipeptide

3

NVNPMTTPVSPAPTVV---IRVSLKVCPTFK

30

+VNP TTP + PT+V I ++ +CPT K

2

DVNPETTPAT--PTIVEATIAATVSICPTTK

30

40.0%

NZ_CP036019.1and 1 other:

NZ_CP036019.1 Lanthipeptides: EXW52_RS27205_lanthipeptide

Lanthipeptides: EXW52_RS27205_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCK

34

+V+P TTP +P T+V+ +S+ +CPT K K

2

DVSPETTPATP--TIVVAISVGLCPTTKCTSK

31

40.0%

NZ_JALAJI010000006.1and 3 others:

NZ_JALAOO010000008.1 Lanthipeptides: MOC69_RS16910_lanthipeptideNZ_JALAXE010000008.1 Lanthipeptides: MOF11_RS14910_lanthipeptideNZ_JALAXI010000013.1 Lanthipeptides: MOE73_RS14185_lanthipeptide

Lanthipeptides: MOB90_RS08905_lanthipeptide

5

NPMTTPVSPAPTVVIRV---SLKVCPTFKIKC

33

N TP S V +R+ S+K CP+FK KC

1

NDEITPQSTVVCVSLRLCKWSIKYCPSFKKKC

32

40.0%

NZ_JALANL010000003.1and 3 others:

NZ_MUEI01000003.1 Lanthipeptides: BV582_RS23575_lanthipeptideNZ_NIJN01000002.1 Lanthipeptides: CHCC5027_RS22460_lanthipeptideNZ_NIMF01000037.1 Lanthipeptides: CHCC14814_RS22115_lanthipeptide

Lanthipeptides: MOC63_RS03255_lanthipeptide

5

NPMTTPVSPAPTVVIRV---SLKVCPTFKIKC

33

N TP S V +R+ S+K CP+FK KC

1

NDEITPQSTVVCVSLRICKWSIKYCPSFKRKC

32

40.0%

NZ_NUML01000015.1:

Lanthipeptides: COD66_RS20140_lanthipeptide

3

NVNPMTTPVSPAPTVVIRVSLKVCPTFKIKCK

34

+V+P TTP +P T+V+ +S+ +CPT K K

2

DVSPETTPATP--TIVVGISVGLCPTTKCTSK

31

38.5%

NZ_CP009280.1and 1 other:

NZ_CP009280.1 Lanthipeptides: P40081_RS37160_lanthipeptide

Lanthipeptides: P40081_RS37160_lanthipeptide

3

NVNPMTTPV--SPAPTVV--IRVSLKVCPTFKIKCK

34

+VNP TTP+ S P V+ + +S +CPT K K

3

DVNPETTPLTTSSWPCVIGAVDISANLCPTTKCTSK

38

MIBiG 4.0 matches for E4T61_RS00570

14.3%

BGC0000553.3 (SSGG_RS09925):

Lanthipeptide: SRO15-2212

20

RVSLKVC

26

+VSL VC

4

QVSLLVC

10

allorf_0098046_0098120

Leader:

MTGARTSGGA

Core:

CGLIRSNAKNLTRS

Core with dehydrations:

CysGLIRDhaNAKNLDhbRDha

antiSMASH-DB 4.0 matches for allorf_0098046_0098120

100.0%

NC_021362.1and 11 others:

NZ_AP025342.1 Lanthipeptides: allorf_1476567_1476641_lanthipeptideNZ_AP026714.1 Lanthipeptides: allorf_1207193_1207267_lanthipeptideNZ_CP010524.1 Lanthipeptides: allorf_0181624_0181698_lanthipeptideNZ_CP020352.1 Lanthipeptides: allorf_0187351_0187425_lanthipeptideNZ_CP023168.1 Lanthipeptides: allorf_0187408_0187482_lanthipeptideNZ_CP039297.1 Lanthipeptides: allorf_0098046_0098120_lanthipeptideNZ_CP040672.1 Lanthipeptides: allorf_1092980_1093054_lanthipeptideNZ_CP059318.1 Lanthipeptides: allorf_0097981_0098055_lanthipeptideNZ_CP087137.1 Lanthipeptides: allorf_0097877_0097951_lanthipeptideNZ_CP118744.1 Lanthipeptides: allorf_0187316_0187390_lanthipeptideNZ_NGMV01000001.1 Lanthipeptides: allorf_1660067_1660141_lanthipeptide

Lanthipeptides: allorf_0181474_0181548_lanthipeptide

1

CGLIRSNAKNLTRS

14

CGLIRSNAKNLTRS

1

CGLIRSNAKNLTRS

14

78.6%

NZ_CP051884.1:

Lanthipeptides: allorf_3277830_3277904_lanthipeptide

1

CGLIRSNAKNLTRS

14

CGLIR NAKNLT++

1

CGLIRCNAKNLTKA

14

allorf_0098279_0098341

Leader:

MTAGDKPEEGG

Core:

DDVKSSCPL

Core with dehydrations:

DDVKDhaDhaCysPL

antiSMASH-DB 4.0 matches for allorf_0098279_0098341

100.0%

NC_021362.1and 9 others:

NZ_AP025342.1 Lanthipeptides: allorf_1476800_1476862_lanthipeptideNZ_CP010524.1 Lanthipeptides: allorf_0181857_0181919_lanthipeptideNZ_CP020352.1 Lanthipeptides: allorf_0187584_0187646_lanthipeptideNZ_CP023168.1 Lanthipeptides: allorf_0187641_0187703_lanthipeptideNZ_CP039297.1 Lanthipeptides: allorf_0098279_0098341_lanthipeptideNZ_CP040672.1 Lanthipeptides: allorf_1093213_1093275_lanthipeptideNZ_CP059318.1 Lanthipeptides: allorf_0098214_0098276_lanthipeptideNZ_CP087137.1 Lanthipeptides: allorf_0098110_0098172_lanthipeptideNZ_CP118744.1 Lanthipeptides: allorf_0187549_0187611_lanthipeptide

Lanthipeptides: allorf_0181707_0181769_lanthipeptide

1

DDVKSSCPL

9

DDVKSSCPL

1

DDVKSSCPL

9

allorf_0098422_0098661

Leader:

MIQPHLPIRLPCYDFTPIICPTFGGWLLKGYLTDFGCYKLSWCDGRCVQGPGTYSPRHADPRLLAIPAS

Core:

RSRVADCDPN

Core with dehydrations:

RDhaRVADCysDPN

antiSMASH-DB 4.0 matches for allorf_0098422_0098661

100.0%

NC_021362.1and 8 others:

NZ_AP025342.1 Lanthipeptides: allorf_1476943_1477149_lanthipeptideNZ_CP010524.1 Lanthipeptides: allorf_0182000_0182206_lanthipeptideNZ_CP020352.1 Lanthipeptides: allorf_0187727_0187933_lanthipeptideNZ_CP023168.1 Lanthipeptides: allorf_0187784_0187990_lanthipeptideNZ_CP039297.1 Lanthipeptides: allorf_0098422_0098661_lanthipeptideNZ_CP040672.1 Lanthipeptides: allorf_1093356_1093595_lanthipeptideNZ_CP059318.1 Lanthipeptides: allorf_0098357_0098596_lanthipeptideNZ_CP118744.1 Lanthipeptides: allorf_0187692_0187898_lanthipeptide

Lanthipeptides: allorf_0181850_0182056_lanthipeptide

1

RSRVADCDPN

10

RSRVADCDPN

1

RSRVADCDPN

10

35.0%

NC_019757.1:

Lanthipeptides: allorf_3742853_3743092_lanthipeptide

1

RSRVADCDPN

10

R RVA CD N

11

RRRVAACDLN

20

allorf_0098925_0099041

Leader:

MDALALGADEGRDEHRYA

Core:

SGSCKQALIRRFPNGETHHS

Core with dehydrations:

DhaGDhaCysKQALIRRFPNGEDhbHHDha

antiSMASH-DB 4.0 matches for allorf_0098925_0099041

100.0%

NZ_CP039297.1and 3 others:

NZ_CP040672.1 Lanthipeptides: allorf_1093859_1093975_lanthipeptideNZ_CP059318.1 Lanthipeptides: allorf_0098860_0098976_lanthipeptideNZ_CP087137.1 Lanthipeptides: allorf_0098698_0098814_lanthipeptide

Lanthipeptides: allorf_0098925_0099041_lanthipeptide

1

SGSCKQALIRRFPNGETHHS

20

SGSCKQALIRRFPNGETHHS

1

SGSCKQALIRRFPNGETHHS

20

30.0%

NZ_CP119804.1:

Lanthipeptides: allorf_1905850_1905972_lanthipeptide

9

IRRFPNGETHHS

20

I RF ++HHS

3

INRFARCDSHHS

14

20.8%

NZ_KZ559472.1:

Lanthipeptides: CXR44_RS39555_lanthipeptide

3

SCKQALIRRFP

13

+CK+ + RFP

8

TCKRTVCSRFP

18

allorf_0099966_0100100

Leader:

MIRLAPVHFRRRVTRPVSYYA

Core:

LFKWWLLLSQHPGCLSNSTSFST

Core with dehydrations:

LFKWWLLLDhaQHPGCysLDhaNDhaDhbDhaFDhaDhb

antiSMASH-DB 4.0 matches for allorf_0099966_0100100

100.0%

NZ_CP039297.1and 3 others:

NZ_CP040672.1 Lanthipeptides: allorf_1094900_1095034_lanthipeptideNZ_CP059318.1 Lanthipeptides: allorf_0099901_0100035_lanthipeptideNZ_CP087137.1 Lanthipeptides: allorf_0099739_0099873_lanthipeptide

Lanthipeptides: allorf_0099966_0100100_lanthipeptide

1

LFKWWLLLSQHPGCLSNSTSFST

23

LFKWWLLLSQHPGCLSNSTSFST

1

LFKWWLLLSQHPGCLSNSTSFST

23

95.7%

NZ_CM000721.1:

Lanthipeptides: allorf_5216161_5216295_lanthipeptide

1

LFKWWLLLSQHPGCLSNSTSFST

23

LFKWWLLLSQHP CLSNSTSFST

1

LFKWWLLLSQHPSCLSNSTSFST

23

19.4%

NC_015711.1:

Lanthipeptides: allorf_5704565_5704741_lanthipeptide

7

LLSQHPGCLSNS

18

L+S +PGCL+++

20

LVSSNPGCLASA

31

with 1 more sequence matches

16.7%

NZ_QKUF01000048.1:

Lanthipeptides: EI42_RS30000_lanthipeptide

12

PGCLSNST

19

PGCL+N+T

9

PGCLTNNT

16

allorf_0101160_0101321

Leader:

MSGGQFDWGGRLLKGNGG

Core:

AQRFPQNGWKSFAECKGTRELDCETYKSSRDESRA

Core with dehydrations:

AQRFPQNGWKDhaFAECysKGDhbRELDCysEDhbYKDhaDhaRDEDhaRA

antiSMASH-DB 4.0 matches for allorf_0101160_0101321

100.0%

NZ_CP039297.1and 3 others:

NZ_CP040672.1 Lanthipeptides: allorf_1096094_1096255_lanthipeptideNZ_CP059318.1 Lanthipeptides: allorf_0101095_0101256_lanthipeptideNZ_CP087137.1 Lanthipeptides: allorf_0100933_0101094_lanthipeptide

Lanthipeptides: allorf_0101160_0101321_lanthipeptide

1

AQRFPQNGWKSFAECKGTRELDCETYKSSRDESRA

35

AQRFPQNGWKSFAECKGTRELDCETYKSSRDESRA

1

AQRFPQNGWKSFAECKGTRELDCETYKSSRDESRA

35

94.3%

NZ_AP026714.1and 4 others:

NZ_CP101913.1 Lanthipeptides: allorf_1818804_1818965_lanthipeptideNZ_KZ845958.1 Lanthipeptides: allorf_1541847_1542008_lanthipeptideNZ_NGMP01000002.1 Lanthipeptides: allorf_002108_002269_lanthipeptideNZ_NGMV01000001.1 Lanthipeptides: allorf_1656789_1656950_lanthipeptide

Lanthipeptides: allorf_1210384_1210545_lanthipeptide

1

AQRFPQNGWKSFAECKGTRELDCETYKSSRDESRA

35

AQRFPQNGWKSF ECKG RELDCETYKSSRDESRA

1

AQRFPQNGWKSFEECKGRRELDCETYKSSRDESRA

35

94.3%

NZ_CM000721.1:

Lanthipeptides: allorf_5214947_5215108_lanthipeptide

1

AQRFPQNGWKSFAECKGTRELDCETYKSSRDESRA

35

AQRFPQNGWKSF ECKG RELDCETYKSSRDESRA

1

AQRFPQNGWKSFVECKGIRELDCETYKSSRDESRA

35

with 2 more sequence matches

91.4%

NC_015215.1:

Lanthipeptides: allorf_0087347_0087508_lanthipeptide

1

AQRFPQNGWKSFAECKGTRELDCETYKSSRDESRA

35

AQRFPQNGWKSFAECKG RELDCE+Y SSRDESRA

1

AQRFPQNGWKSFAECKGIRELDCESYNSSRDESRA

35

22.0%

NZ_FOBB01000013.1:

Lanthipeptides: BMZ54_RS31540_lanthipeptide

3

RFPQNGWKSFAECKGTRELDCET

25

P G S+AEC T + C T

13

NIPILGAISYAECASTGQPTCNT

35

allorf_0101424_0101654

Leader:

MAPRCRLIASWGCSRSQGLGCSPIKAVRELGSERRETVRSLSVAGA

Core:

GNLRGAVLSTRGPGWTHRWCTSCSAKGIAG

Core with dehydrations:

GNLRGAVLDhaDhbRGPGWDhbHRWCysDhbDhaCysDhaAKGIAG

antiSMASH-DB 4.0 matches for allorf_0101424_0101654

100.0%

NZ_CP039297.1and 3 others:

NZ_CP040672.1 Lanthipeptides: allorf_1096358_1096588_lanthipeptideNZ_CP059318.1 Lanthipeptides: allorf_0101359_0101589_lanthipeptideNZ_CP087137.1 Lanthipeptides: allorf_0101197_0101427_lanthipeptide

Lanthipeptides: allorf_0101424_0101654_lanthipeptide

1

GNLRGAVLSTRGPGWTHRWCTSCSAKGIAG

30

GNLRGAVLSTRGPGWTHRWCTSCSAKGIAG

1

GNLRGAVLSTRGPGWTHRWCTSCSAKGIAG

30

80.0%

NZ_CM000721.1:

Lanthipeptides: allorf_5214614_5214844_lanthipeptide

7

VLSTRGPGWTHRWCTSCSAKGIAG

30

VLSTRGPGWTHRWCTSCSAKGIAG

1

VLSTRGPGWTHRWCTSCSAKGIAG

24

76.7%

NZ_AP026714.1and 4 others:

NZ_KZ845958.1 Lanthipeptides: allorf_1541514_1541744_lanthipeptideNZ_NGMP01000002.1 Lanthipeptides: allorf_002372_002602_lanthipeptideNZ_NGMP01000002.1 Lanthipeptides: allorf_002372_002602_lanthipeptideNZ_NGMV01000001.1 Lanthipeptides: allorf_1656456_1656686_lanthipeptide

Lanthipeptides: allorf_1210648_1210878_lanthipeptide

7

VLSTRGPGWTHRWCTSCSAKGIAG

30

VLSTRGPGWT+RWCTSCSAKGIAG

1

VLSTRGPGWTYRWCTSCSAKGIAG

24

with 6 more sequence matches

61.4%

NZ_CP101913.1:

Lanthipeptides: allorf_1819068_1819298_lanthipeptide

1

GNLRGAVLSTRGPGWTHRWCTSCSAKGIAG

30

G+LRGAVLSTRGPGWTHRWCTSCSA+ IAG

15

GSLRGAVLSTRGPGWTHRWCTSCSARSIAG

44

54.5%

NC_015215.1:

Lanthipeptides: allorf_0087611_0087841_lanthipeptide

1

GNLRGAVLSTRGPGWTHRWCTSCSAKGIAG

30

GNLRG+ STRGP WT+RWCTSC AKGIAG

15

GNLRGSAPSTRGPEWTYRWCTSCLAKGIAG

44

30.0%

NZ_CP025546.1and 5 others:

NZ_CP025546.1 Lanthipeptides: allorf_4260056_4260220_lanthipeptideNZ_SDLM01000003.1 Lanthipeptides: allorf_121827_121991_lanthipeptideNZ_SDLM01000003.1 Lanthipeptides: allorf_121827_121991_lanthipeptideNZ_SDLN01000007.1 Lanthipeptides: allorf_135103_135267_lanthipeptideNZ_SDLN01000007.1 Lanthipeptides: allorf_135103_135267_lanthipeptide

Lanthipeptides: allorf_4260056_4260220_lanthipeptide

9

STRGPGWTHRWCTSCSAKG

27

+ RGP W W T CS G

3

TKRGPTWDPLWATPCSLNG

21

20.0%

NZ_JANIOB010000033.1:

Lanthipeptides: NQ113_RS28010_lanthipeptide

1

GNLRGAVLSTRGPGWTHRWCTS

22

G++ PG+T+R C +

1

GDVNPETTPVINPGFTNRLCPT

22

14.6%

NZ_JACHJJ010000004.1:

Lanthipeptides: FHS22_RS08790_lanthipeptide

8

LSTRGPGWTHRWCTSCS

24

++ GPG++ R T+CS

24

ITMTGPGYSGRCGTACS

40

13.9%

NZ_CP041611.1and 1 other:

NZ_CP041613.2 Lanthipeptides: allorf_01201490_01201714_lanthipeptide

Lanthipeptides: allorf_00809662_00809886_lanthipeptide

12

GPGWTH

17

GPGW+H

9

GPGWSH

14

MIBiG 4.0 matches for allorf_0101424_0101654

13.3%

BGC0001155.5 (AGY49587.1):

Thiopeptide: GE2270

19

WCTSCS

24

+C SCS

8

FCCSCS

13

allorf_0101584_0101730

Leader:

MRNGKSHLEGG

Core:

FMLRCFQHLSRPHIATQRCPWQNNWYTSGASIPVLSY

Core with dehydrations:

FMLRCysFQHLDhaRPHIADhbQRCysPWQNNWYDhbDhaGADhaIPVLDhaY

antiSMASH-DB 4.0 matches for allorf_0101584_0101730

100.0%

NZ_CP039297.1and 3 others:

NZ_CP040672.1 Lanthipeptides: allorf_1096518_1096664_lanthipeptideNZ_CP059318.1 Lanthipeptides: allorf_0101519_0101665_lanthipeptideNZ_CP087137.1 Lanthipeptides: allorf_0101357_0101503_lanthipeptide

Lanthipeptides: allorf_0101584_0101730_lanthipeptide

1

FMLRCFQHLSRPHIATQRCPWQNNWYTSGASIPVLSY

37

FMLRCFQHLSRPHIATQRCPWQNNWYTSGASIPVLSY

1

FMLRCFQHLSRPHIATQRCPWQNNWYTSGASIPVLSY

37

24.4%

NZ_CP070036.1and 2 others:

NZ_JADMPO010000043.1 Lanthipeptides: I2I27_RS16845_lanthipeptideNZ_JAQMLK010000037.1 Lanthipeptides: PNW18_RS16700_lanthipeptide

Lanthipeptides: I6K72_RS03930_lanthipeptide

2

MLRCFQHLSRPHIATQRCPWQNNW

25

M R Q LS H RC W+ +W

10

MERIKQLLS-EHSQMFRCSWRKSW

32

20.5%

NZ_BMRY01000003.1and 1 other:

NZ_BMRY01000003.1 Lanthipeptides: allorf_458543_458737_lanthipeptide

Lanthipeptides: allorf_458543_458737_lanthipeptide

3

LRC-FQHLSRPHIATQRCPW

21

LRC F P + RCPW

13

LRCPFVRPPSPLLLRPRCPW

32

with 3 more sequence matches

18.9%

NZ_LT630004.1:

Lanthipeptides: allorf_2780249_2780374_lanthipeptide

19

CPWQNNWYTSGASIPV

34

CP++NN Y G +P+

11

CPYRNNIY--GTFLPL

24

18.0%

NZ_JACKVL010000013.1:

Lanthipeptides: allorf_1185244_1185420_lanthipeptide

9

LSRPHIATQRCPWQNNWYTSGASIPV

34

L R +A +R P +W TS +I +

4

LVRLAVAAKRMPAGASWTTSTGNIEI

29

13.5%

NZ_LR134301.1:

Lanthipeptides: allorf_1738566_1738781_lanthipeptide

19

CPWQNNWYTSG

29

CPW W G

4

CPWPGGWVVQG

14

allorf_0101837_0102064

Leader:

MYAYCYLVLREHSQWFGG

Core:

DSEEVTPVPMPNTEVKLFSADGSRGFPPVRVGRRQAIITVKAAHHELPFLCFSAYVI

Core with dehydrations:

DDhaEEVDhbPVPMPNDhbEVKLFDhaADGDhaRGFPPVRVGRRQAIIDhbVKAAHHELPFLCysFDhaAYVI

antiSMASH-DB 4.0 matches for allorf_0101837_0102064

100.0%

NZ_CP039297.1and 1 other:

NZ_CP087137.1 Lanthipeptides: allorf_0101610_0101837_lanthipeptide

Lanthipeptides: allorf_0101837_0102064_lanthipeptide

1

DSEEVTPVPMPNTEVKLFSADGSRGFPPVRVGRRQAIITVKAAHHELPFLCFSAYVI

57

DSEEVTPVPMPNTEVKLFSADGSRGFPPVRVGRRQAIITVKAAHHELPFLCFSAYVI

1

DSEEVTPVPMPNTEVKLFSADGSRGFPPVRVGRRQAIITVKAAHHELPFLCFSAYVI

57

98.2%

NZ_CP040672.1:

Lanthipeptides: allorf_1096771_1096998_lanthipeptide

1

DSEEVTPVPMPNTEVKLFSADGSRGFPPVRVGRRQAIITVKAAHHELPFLCFSAYVI

57

DSEEVTPVPMPNTEVKLFSADGSRGFPPVRVGRRQAII+VKAAHHELPFLCFSAYVI

1

DSEEVTPVPMPNTEVKLFSADGSRGFPPVRVGRRQAIISVKAAHHELPFLCFSAYVI

57

98.2%

NZ_CP059318.1:

Lanthipeptides: allorf_0101772_0101999_lanthipeptide

1

DSEEVTPVPMPNTEVKLFSADGSRGFPPVRVGRRQAIITVKAAHHELPFLCFSAYVI

57

DSEEVTPVPMPNTEVKLFSADGSRGF PVRVGRRQAIITVKAAHHELPFLCFSAYVI

1

DSEEVTPVPMPNTEVKLFSADGSRGFLPVRVGRRQAIITVKAAHHELPFLCFSAYVI

57

allorf_0102024_0102224

Leader:

MSCLFCVFLHTLYNM

Core:

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNKGGG

Core with dehydrations:

RNVDhbICysLFAKAGFMLVQDhaGNNRDRAIQLKNFAKHLIVRLKDhaNIVKVNKGGG

antiSMASH-DB 4.0 matches for allorf_0102024_0102224

100.0%

NZ_CP039297.1:

Lanthipeptides: allorf_0102024_0102224_lanthipeptide

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNKGGG

51

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNKGGG

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNKGGG

51

98.0%

NZ_CP040378.1:

Lanthipeptides: allorf_0072224_0072424_lanthipeptide

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNKGGG

51

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKH IVRLKSNIVKVNKGGG

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHFIVRLKSNIVKVNKGGG

51

98.0%

NZ_CP040672.1:

Lanthipeptides: allorf_1096958_1097158_lanthipeptide

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNKGGG

51

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAK LIVRLKSNIVKVNKGGG

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKRLIVRLKSNIVKVNKGGG

51

with 4 more sequence matches

98.0%

NZ_CP059318.1:

Lanthipeptides: allorf_0101959_0102159_lanthipeptide

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNKGGG

51

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVN+GGG

1

RNVTICLFAKAGFMLVQSGNNRDRAIQLKNFAKHLIVRLKSNIVKVNEGGG

51

15.7%

NZ_CP011130.1:

Lanthipeptides: allorf_4095947_4096132_lanthipeptide

9

AKAGFMLVQSGNNRDR

24

AKA + +SG +RDR

9

AKATWPTCRSGASRDR

24

15.7%

NZ_CP023465.1:

Lanthipeptides: allorf_4137774_4138001_lanthipeptide

9

AKAGFMLVQSGNNRDRAI

26

A+A + +SG +RDR I

8

AEATWPTCRSGASRDRDI

25

11.3%

NZ_VITV01000003.1:

Lanthipeptides: FBZ87_RS11200_lanthipeptide

6

CLFAKAGFMLV

16

C AKAGFM++

49

CDSAKAGFMVI

59

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 16.98 out of 18.16

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.30 out of 27.78

Confidence: medium

Additional weak hits (1):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 25.02 out of 36.54

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

E4T61_RS00570 - Class II

Cleavage pHMM score: -3.30

RODEO score: 0

Monoisotopic mass: 3716.1 Da

Molecular weight: 3718.5 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3736.5 Da N = 1; 3754.5 Da N = 2; 3772.5 Da N = 3; 3790.6 Da N = 4

allorf_0098046_0098120 - Class II

Cleavage pHMM score: -3.80

RODEO score: 0

Monoisotopic mass: 1477.8 Da

Molecular weight: 1478.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1496.7 Da N = 1; 1514.7 Da N = 2

allorf_0098279_0098341 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 926.4 Da

Molecular weight: 927.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 945.0 Da N = 1

allorf_0098422_0098661 - Class II

Cleavage pHMM score: -3.90

RODEO score: 0

Monoisotopic mass: 1113.5 Da

Molecular weight: 1114.2 Da

Number of bridges: 1

allorf_0098925_0099041 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 2152.1 Da

Molecular weight: 2153.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2171.4 Da N = 1; 2189.4 Da N = 2; 2207.4 Da N = 3

allorf_0099966_0100100 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 2525.3 Da

Molecular weight: 2526.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2544.9 Da N = 1; 2562.9 Da N = 2; 2580.9 Da N = 3; 2599.0 Da N = 4; 2617.0 Da N = 5; 2635.0 Da N = 6

allorf_0101160_0101321 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 3971.9 Da

Molecular weight: 3974.3 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3992.3 Da N = 1; 4010.3 Da N = 2; 4028.3 Da N = 3; 4046.4 Da N = 4

allorf_0101424_0101654 - Class II

Cleavage pHMM score: -2.30

RODEO score: 0

Monoisotopic mass: 2990.5 Da

Molecular weight: 2992.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3010.4 Da N = 1; 3028.4 Da N = 2; 3046.4 Da N = 3; 3064.5 Da N = 4

allorf_0101584_0101730 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 4299.1 Da

Molecular weight: 4301.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 4319.9 Da N = 1; 4337.9 Da N = 2; 4356.0 Da N = 3; 4374.0 Da N = 4

allorf_0101837_0102064 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 6152.3 Da

Molecular weight: 6156.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 6174.0 Da N = 1; 6192.1 Da N = 2; 6210.1 Da N = 3; 6228.1 Da N = 4; 6246.1 Da N = 5; 6264.1 Da N = 6

allorf_0102024_0102224 - Class II

Cleavage pHMM score: -5.60

RODEO score: 0

Monoisotopic mass: 5582.2 Da

Molecular weight: 5585.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5603.6 Da N = 1; 5621.6 Da N = 2

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria

NZ_CP039297.1 - Region 2 - NRPS

Download region GenBank file

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Location: 324,128 - 389,535 nt. (total: 65,408 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase electron transfer oxidoreductase reductase

E4T61_RS01735: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

E4T61_RS01750: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

E4T61_RS01755: flavodoxin

smcogs: SMCOG1134: flavodoxin

E4T61_RS01760: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

E4T61_RS01775: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Transferases: PPTase aminotransferase

E4T61_RS01840: aminotransferase

smcogs: SMCOG1019: aminotransferase

E4T61_RS01855: aminotransferase

smcogs: SMCOG1019: aminotransferase

E4T61_RS01860: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase

E4T61_RS01835: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

E4T61_RS01770: cobalamin synthesis protein/P47K family protein

smcogs: SMCOG1178: cobalamin synthesis protein/P47K family protein

E4T61_RS01820: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E4T61_RS01830: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E4T61_RS19825: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E4T61_RS19835: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000433.5	0.92	NRPS	surfactin	Bacillus velezensis FZB42
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000433.5	0.92	NRPS	surfactin	Bacillus velezensis FZB42
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.55 out of 29.38

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 24.80 out of 27.78

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.40 out of 20.97

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.50 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.76 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.41 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.42 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.88 out of 26.06

Confidence: medium

Additional weak hits (17):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.89 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.74 out of 32.74

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.52 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 19.84 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.40 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.84 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.78 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.38 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.16 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.24 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 19.99 out of 26.06

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 22.63 out of 36.54

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 21.56 out of 31.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.35 out of 30.25

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 23.04 out of 30.12

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 22.50 out of 35.63

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.97 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 324127 - 389535:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - Leu - D-Leu) + (Val) + (Asp - D-Leu) + (Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

E4T61_RS01820: Glu - Leu - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (465..867): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: LSLAFDASVKQADGLIGGETNVYGPTETCVDASV
Nearest Stachelhaus code(s):: Glu DAKDLGVVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1511..1908): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: TNTSFDAFMFDGMIMFGGELHMYGPSESTVFATY
Nearest Stachelhaus code(s):: Leu DAFMMGMVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2550..2939): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDASIWEPFLLVGGDVNNYGPTENTVVATS
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS19825: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (452..846): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LHSGFDAGTFEGWLIIGGDWNGYGPTENTTFSTS
Nearest Stachelhaus code(s):: Val DAFWIGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS19835: Asp - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (429..831): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YWFSFDLGYTAPKLVLGGEINHYGPTETTIGAIA
Nearest Stachelhaus code(s):: Asp DLTKVGHIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1474..1863): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDASIWEPFLLVGGDVNNYGPTENTVVATS
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS01830: Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LHVSFDAFTFDAFALFGGEINCYGPTEGTVFATA
Nearest Stachelhaus code(s):: Leu DAFFLGCVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 1	Ferric Uptake Regulator
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis

NZ_CP039297.1 - Region 3 - PKS-like

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Location: 966,270 - 1,007,514 nt. (total: 41,245 nt) Show pHMM detection rules used

PKS-like: (t2pks2 or ksIII)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene overview

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TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

E4T61_RS05040: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase aminotransferase

E4T61_RS05070: acyltransferase

smcogs: SMCOG1141: acyltransferase

E4T61_RS05085: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: deacetylase

E4T61_RS05130: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

Ligases: synthase

E4T61_RS05090: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: permease

E4T61_RS05045: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.55	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001928.2	0.50	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002404.2	0.45	other (other)	falcarindiol	Solanum lycopersicum
BGC0002294.3	0.44	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000205.3	0.43	PKS	bryostatin	Candidatus Endobugula sertula
BGC0002150.2	0.42	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.42	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0001292.5	0.42	other (other)	A33853	Streptomyces sp. NRRL 12068
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001206.5	0.42	other (other)	indolmycin	Streptomyces griseus subsp. griseus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.55	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001928.2	0.50	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002404.2	0.45	other (other)	falcarindiol	Solanum lycopersicum
BGC0002294.3	0.44	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000205.3	0.43	PKS	bryostatin	Candidatus Endobugula sertula
BGC0002150.2	0.42	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.42	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0001292.5	0.42	other (other)	A33853	Streptomyces sp. NRRL 12068
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001206.5	0.42	other (other)	indolmycin	Streptomyces griseus subsp. griseus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.15 out of 18.46

Confidence: strong

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 25.91 out of 27.55

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.75 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.81 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.88 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.01 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.52 out of 26.06

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.50 out of 23.79

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 21.49 out of 25.88

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 23.85 out of 28.19

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.27 out of 28.19

Confidence: medium

Additional weak hits (11):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.48 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.02 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.28 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 23.97 out of 28.14

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.34 out of 30.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.28 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.75 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.41 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 25.42 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 22.32 out of 33.14

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 20.59 out of 30.11

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
GnfM	Regulator of nitrogen-fixation genes
IolR	Regulator of the inositol catabolism
MogR	Repressor of flagellar motility genes
PsrA	Oleic acid responsive regulator
RpoN	Regulator of nitrogen assimilation and virulence
ZuR variant 1	Zinc-responsive repressor

NZ_CP039297.1 - Region 4 - terpene

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Location: 1,090,315 - 1,111,055 nt. (total: 20,741 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

E4T61_RS05725: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: hydrolase

E4T61_RS05695: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002458.3	0.45	NRPS	alazopeptin	Streptacidiphilus griseoplanus
BGC0000885.3	0.44	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000846.5	0.44	other (other)	tabtoxin	Pseudomonas syringae
BGC0001880.3	0.41	ribosomal	scytodecamide	Scytonema sp. UIC 10036
BGC0001374.5	0.39	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001488.3	0.36	other (other)	4-formylaminooxyvinylglycine	Pseudomonas fluorescens WH6
BGC0000254.5	0.33	PKS	oxytetracycline	Streptomyces rimosus
BGC0000442.5	0.33	NRPS	terrequinone A	Aspergillus nidulans FGSC A4
BGC0001007.5	0.32	NRPS, PKS	lymphostin, neolymphostinol B, lymphostinol, neolymphostin b	Salinispora arenicola CNS-205
BGC0000937.5	0.32	other (other)	fosfazinomycin A, fosfazinomycin B	Streptomyces sp. WM6372

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002458.3	0.45	NRPS	alazopeptin	Streptacidiphilus griseoplanus
BGC0000885.3	0.44	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000846.5	0.44	other (other)	tabtoxin	Pseudomonas syringae
BGC0001880.3	0.41	ribosomal	scytodecamide	Scytonema sp. UIC 10036
BGC0001374.5	0.39	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001488.3	0.36	other (other)	4-formylaminooxyvinylglycine	Pseudomonas fluorescens WH6
BGC0000254.5	0.33	PKS	oxytetracycline	Streptomyces rimosus
BGC0000442.5	0.33	NRPS	terrequinone A	Aspergillus nidulans FGSC A4
BGC0001007.5	0.32	NRPS, PKS	lymphostin, neolymphostinol B, lymphostinol, neolymphostin b	Salinispora arenicola CNS-205
BGC0000937.5	0.32	other (other)	fosfazinomycin A, fosfazinomycin B	Streptomyces sp. WM6372

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene		squalene	C30	acyclic	none
triterpene		PSPP	C30	acyclic	PP
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

E4T61_RS05700: PT_phytoene_like

Domain 1 (3 - 242): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → PSPP; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.10 out of 26.85

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.88 out of 27.78

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 19.23 out of 20.97

Confidence: strong

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 23.16 out of 26.33

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.46 out of 27.78

Confidence: medium

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 26.99 out of 35.63

Confidence: medium

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.96 out of 32.93

Confidence: medium

Additional weak hits (4):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.91 out of 32.74

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.16 out of 26.06

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.61 out of 30.22

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 3	Repressor of DNA damage response
NrtR	NAD synthesis repressor
RutR	Regulator of the pyrimidine and purine metabolism

NZ_CP039297.1 - Region 5 - transAT-PKS

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Location: 1,416,078 - 1,504,311 nt. (total: 88,234 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

E4T61_RS07535: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS07545: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS07550: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS07560: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS07590: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Transferases: acylase kinase

E4T61_RS07420: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

E4T61_RS07530: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: deacetylase deformylase hydrolase phosphatase

E4T61_RS07505: hydrolase

smcogs: SMCOG1179: hydrolase

E4T61_RS07510: peptide deformylase

smcogs: SMCOG1275: peptide deformylase

E4T61_RS07565: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

E4T61_RS07570: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

E4T61_RS07600: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

E4T61_RS07635: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

Ligases: synthase

E4T61_RS07540: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E4T61_RS07580: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000181.5	0.93	PKS	macrolactin H	Bacillus velezensis FZB42
BGC0001383.3	0.92	PKS	macrolactin H, macrolactin B, macrolactin 1c, macrolactin E	Jeotgalibacillus marinus
BGC0001534.3	0.70	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001284.4	0.63	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000181.5	0.93	PKS	macrolactin H	Bacillus velezensis FZB42
BGC0001383.3	0.92	PKS	macrolactin H, macrolactin B, macrolactin 1c, macrolactin E	Jeotgalibacillus marinus
BGC0001534.3	0.70	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001284.4	0.63	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.90 out of 27.78

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.94 out of 21.55

Confidence: medium

Additional weak hits (6):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.30 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.56 out of 29.38

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.35 out of 26.33

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 23.20 out of 31.73

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 22.99 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 5, location 1416077 - 1504311:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ohmal - ccmal - ccmal) + (ccmal) + (ccmal - mal) + (ccmal - mal) + (ohmal) + (ohmal - mal)
Non-colinear ordering:: E4T61_RS07535 + E4T61_RS07540 + E4T61_RS07545 + E4T61_RS07550 + E4T61_RS07555 + E4T61_RS07560 + E4T61_RS07565

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

E4T61_RS07530: mal

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 91.9
inactive: 31.2
Methoxymalonyl-CoA: 26.1
Methylmalonyl-CoA: 19.6
Propionyl-CoA: 16.9
fatty_acid: 13.9
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ToxT	Regulator of MSHA biosynthesis

NZ_CP039297.1 - Region 6 - NRPS,T3PKS,transAT-PKS

Download region GenBank file

Download region SVG

Location: 1,723,019 - 1,833,118 nt. (total: 110,100 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

E4T61_RS08780: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

E4T61_RS08865: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS08885: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase

E4T61_RS08825: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

E4T61_RS08830: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

E4T61_RS08835: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: hydrolase protease

E4T61_RS08760: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

E4T61_RS08820: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

E4T61_RS08905: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: hydratase

E4T61_RS08850: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

E4T61_RS08855: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

E4T61_RS08785: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

E4T61_RS08845: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

E4T61_RS08860: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E4T61_RS08870: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E4T61_RS08875: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E4T61_RS08880: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001089.5	1.75	NRPS, PKS	bacillaene	Bacillus velezensis FZB42
BGC0001160.5	1.04	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002135.2	1.03	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001164.3	1.01	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001132.4	0.97	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.97	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.96	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.96	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.94	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.94	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001089.5	0.93	NRPS, PKS	bacillaene	Bacillus velezensis FZB42
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.04 out of 18.57

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.79 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.46 out of 27.78

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 19.78 out of 20.97

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.62 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.14 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.02 out of 27.78

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.47 out of 21.55

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.29 out of 27.78

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 24.56 out of 24.78

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.30 out of 24.78

Confidence: medium

Additional weak hits (16):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.51 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.96 out of 26.06

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 24.16 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.22 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.08 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.45 out of 26.06

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 26.07 out of 39.62

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.49 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.06 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.04 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.39 out of 30.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 24.54 out of 36.54

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 20.38 out of 28.14

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.62 out of 32.93

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 21.58 out of 26.12

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.08 out of 39.62

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 6, location 1723018 - 1833118:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - Gly - ccmal - ohmal - mal) + (ccmal - mal - ohmal - mal) + (Me-ccmal - ohmal) + (Ala - ccmal - ccmal - Me-ccmal) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

E4T61_RS08825: mal

PKS_AT (4..285): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 101.1
inactive: 46.8
Methoxymalonyl-CoA: 38.0
Methylmalonyl-CoA: 24.9
Propionyl-CoA: 23.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

E4T61_RS08830: mal

PKS_AT (7..293): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 31.8
inactive: 26.4
Methoxymalonyl-CoA: 15.2
Benzoyl-CoA: 14.0
Isobutyryl-CoA: 8.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

E4T61_RS08835: mal

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 92.8
inactive: 28.2
Methoxymalonyl-CoA: 23.4
Methylmalonyl-CoA: 11.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

E4T61_RS08860: fatty_acid - Gly

Search NORINE for peptide: strict or relaxed

CAL_domain (74..476): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 26.7
NH2: 9.4
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1145..1540): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDIAALELQALCGGEWNMFGPTETTIWSVV
Nearest Stachelhaus code(s):: Gly DILQLGMIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS08875: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (492..896): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TFLTFDVSISEGNFMVAGEENIYGPTETSIYAAY
Nearest Stachelhaus code(s):: Ala DVSNMAIIYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 2	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis

NZ_CP039297.1 - Region 7 - NRPS,betalactone,transAT-PKS

Download region GenBank file

Download region SVG

Location: 1,895,285 - 2,033,109 nt. (total: 137,825 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

E4T61_RS09355: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

E4T61_RS09370: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS09470: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS09490: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

E4T61_RS09565: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

E4T61_RS09670: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase aminotransferase carboxylase transferase

E4T61_RS09465: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

E4T61_RS09510: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

E4T61_RS09525: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

E4T61_RS09535: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

E4T61_RS09555: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Hydrolases: peptidase

E4T61_RS09645: gamma-glutamyltranspeptidase

smcogs: SMCOG1274: gamma-glutamyltranspeptidase

Lyases: hydratase

E4T61_RS09540: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

E4T61_RS09450: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E4T61_RS09455: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E4T61_RS09460: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E4T61_RS09505: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

E4T61_RS09545: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

E4T61_RS09560: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E4T61_RS09580: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E4T61_RS09585: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E4T61_RS09590: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E4T61_RS09595: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

E4T61_RS09600: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001098.5	1.68	NRPS, PKS	iturin	Bacillus subtilis
BGC0002135.2	1.59	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	1.58	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001095.5	1.56	NRPS	fengycin	Bacillus velezensis FZB42
BGC0001090.5	1.53	NRPS, PKS	bacillomycin D	Bacillus velezensis FZB42
BGC0001128.4	1.51	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	1.49	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	1.49	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	1.49	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	1.47	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001095.5	0.94	NRPS	fengycin	Bacillus velezensis FZB42
BGC0001098.5	0.90	NRPS, PKS	iturin	Bacillus subtilis
BGC0001090.5	0.88	NRPS, PKS	bacillomycin D	Bacillus velezensis FZB42
BGC0000407.5	0.81	NRPS	plipastatin	Bacillus subtilis subsp. subtilis
BGC0000402.4	0.81	NRPS	paenilarvin A, paenilarvin B, paenilarvin C	Paenibacillus larvae subsp. larvae DSM 25430
BGC0001103.5	0.79	NRPS, PKS	mycosubtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.15 out of 18.46

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.59 out of 27.78

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 22.29 out of 27.55

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 20.32 out of 20.97

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.35 out of 24.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.38 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.67 out of 26.06

Confidence: medium

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.44 out of 32.93

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.67 out of 26.06

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.67 out of 20.01

Confidence: medium

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 22.93 out of 29.25

Confidence: medium

Additional weak hits (11):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.13 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.15 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.52 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.45 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.46 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.74 out of 26.06

Confidence: weak

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.44 out of 20.47

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.79 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.75 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.41 out of 29.38

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.89 out of 30.25

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 7, location 1895284 - 2033109:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - D-Orn) + (Tyr - D-Thr) + (Glu - D-X) + (Pro - Gln - D-Tyr) + (Ile) + (fatty_acid - mal - Asn - D-Tyr) + (D-Asn - Gln - Pro - D-Asn) + (Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 8, location 1895284 - 1972533:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - mal - Asn - D-Tyr) + (D-Asn - Gln - Pro - D-Asn) + (Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 9, location 1955439 - 2033109:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - D-Orn) + (Tyr - D-Thr) + (Glu - D-X) + (Pro - Gln - D-Tyr) + (Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

E4T61_RS09450: Asn - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (268..663): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTITSTKLIVGGDFNEYGPTETVVGCMI
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2185): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFASGEINLYGPTEATIDVSY
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS09455: Tyr - Asn - Gln - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (268..662): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: SWKYFDAFILNGSTVVGGENNEYGPTENSVVSTF
Nearest Stachelhaus code(s):: Tyr DALSVGEVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1779..2174): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTSTKLIVGGECNEYGPTETVVGCMI
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3288..3693): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: LGLAFDASVQQADGLIGGETNVYGPTETCVDASL
Nearest Stachelhaus code(s):: Gln DAQDLGVVDK (100% 8Å match); Ile DAQDLGVVDK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4337..4733): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LYEAFDVCYQESFLIAAGEHNHYGPSETHVVTMY
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS09460: fatty_acid - Asn

Search NORINE for peptide: strict or relaxed

CAL_domain (36..478): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 624.6
NH2: 18.6
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (2956..3351): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTSTKLIVGGEFNEYGPTEAVVGCMI
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS09465: mal

PKS_AT (6..290): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.7
inactive: 30.0
Methoxymalonyl-CoA: 20.4
Propionyl-CoA: 11.8
Methylmalonyl-CoA: 11.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

E4T61_RS09560: X

AMP-binding (12..445): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NNIVF-GCVLGAHGIMAGSTIAYGQTEASPVITQ
Nearest Stachelhaus code(s):: Z-ahmohOrn FGLHIASTCK (56% 8Å match); Ile DGLFIGIPVK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

E4T61_RS09580: Ile

Search NORINE for peptide: strict or relaxed

AMP-binding (473..866): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: LNSGFDAVTFEGFLYVGGEYNIYGPTENTTFSTF
Nearest Stachelhaus code(s):: Ile DAFFYGITFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS09585: Pro - Gln - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (469..866): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LHQAFDVCYQESFFITAGEHNHYGPSETHVVSTY
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1500..1901): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: FSLTFDASIWEWHFFAGGETNSYGPTEATVEAAY
Nearest Stachelhaus code(s):: Gln DAWHFGSVEK (100% 8Å match); Glu DAWHFGSVEK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2532..2924): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: TWRFFDGFVTSTIITLAGEANEYGPTENSVATTA
Nearest Stachelhaus code(s):: Tyr DGTITAEVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS09590: Glu - X

Search NORINE for peptide: strict or relaxed

AMP-binding (468..873): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: FGMSFDASIWQWHFFAGGEIHGYGPTEATVDAAF
Nearest Stachelhaus code(s):: Glu DAWHFGGVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1505..1898): X

nrpys: Ala, Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LNSSFDVTTFEGWLIIGGEWNGYGPTENTTFSTC
Nearest Stachelhaus code(s):: Ala or Val DVFWIGGTFK (97% 8Å match); Val DVFWIGGTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS09595: Tyr - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (474..867): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: TWRFFDGSLTSTLITFAGELNEYGPTENSVATTI
Nearest Stachelhaus code(s):: Tyr DGTLTAEVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1509..1905): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LHKHFDFSVWEGNQIFGGEVNMYGITETTVHVTC
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS09600: Glu - Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (463..868): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: FGMSFDASIWQWHFFAGGEIHGYGPTEATVDAAF
Nearest Stachelhaus code(s):: Glu DAWHFGGVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1500..1905): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: AAWAFDVFSGDRESVLGSDINSYGVTEATIDSSY
Nearest Stachelhaus code(s):: Orn DVGEVGSIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
GnfM	Regulator of nitrogen-fixation genes
LexA variant 2	Repressor of DNA damage response
LexA variant 3	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
RpoN	Regulator of nitrogen assimilation and virulence
SypG	Regulator of biofilm formation and host colonization
ToxT	Regulator of MSHA biosynthesis

NZ_CP039297.1 - Region 8 - terpene

Download region GenBank file

Download region SVG

Location: 2,057,217 - 2,079,100 nt. (total: 21,884 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

E4T61_RS09900: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Hydrolases: hydrolase

E4T61_RS09925: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002173.2	0.47	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0001535.3	0.42	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000313.4	0.36	NRPS	beauvericin	Beauveria bassiana
BGC0002255.3	0.33	PKS	lijiquinone	Ascomycota sp. F53
BGC0000663.5	0.30	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000686.3	0.30	terpene	helvolic acid	Aspergillus fumigatus Af293
BGC0001575.4	0.29	NRPS	PZN5, PZN6, PZN7, PZN9, PZN10, PZN11	Ruminococcus sp. 5_1_39BFAA
BGC0001338.4	0.28	PKS	citrinin	Monascus ruber
BGC0000154.4	0.28	PKS	stipitatic acid	Talaromyces stipitatus ATCC 10500
BGC0001739.3	0.28	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002173.2	0.47	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0001535.3	0.42	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000313.4	0.36	NRPS	beauvericin	Beauveria bassiana
BGC0002255.3	0.33	PKS	lijiquinone	Ascomycota sp. F53
BGC0000663.5	0.30	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000686.3	0.30	terpene	helvolic acid	Aspergillus fumigatus Af293
BGC0001575.4	0.29	NRPS	PZN5, PZN6, PZN7, PZN9, PZN10, PZN11	Ruminococcus sp. 5_1_39BFAA
BGC0001338.4	0.28	PKS	citrinin	Monascus ruber
BGC0000154.4	0.28	PKS	stipitatic acid	Talaromyces stipitatus ATCC 10500
BGC0001739.3	0.28	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

E4T61_RS09905: T2TS

Domain 1 (5 - 625): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 17.14 out of 18.16

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.04 out of 18.57

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 26.30 out of 27.78

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.53 out of 26.06

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.86 out of 27.78

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.70 out of 28.19

Confidence: medium

Additional weak hits (7):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.62 out of 21.55

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.90 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.10 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.37 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.54 out of 26.06

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.14 out of 39.62

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.63 out of 25.75

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NZ_CP039297.1 - Region 9 - T3PKS

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Location: 2,142,426 - 2,183,526 nt. (total: 41,101 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase methyltransferase

E4T61_RS10465: isoprenylcysteine carboxyl methyltransferase

smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

E4T61_RS10475: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: hydrolase

E4T61_RS10490: isochorismatase

smcogs: SMCOG1027: isochorismatase

Ligases: synthase

E4T61_RS10470: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.55	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001831.3	0.54	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002332.2	0.44	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0001475.4	0.43	terpene	aspterric acid	Aspergillus terreus NIH2624
BGC0001289.5	0.35	ribosomal	penisin	Paenibacillus ehimensis
BGC0001647.3	0.34	PKS	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0002254.3	0.33	PKS	notoamide F, (+)-semivioxanthin, waikikiamide B, waikikiamide C, notoamide A, waikikiamide A	Aspergillus sp. FM242
BGC0001964.2	0.33	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001928.2	0.32	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0001663.3	0.32	PKS	merocyclophane C, merocyclophane D	Nostoc sp. UIC 10110

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.55	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001831.3	0.54	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002332.2	0.44	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0001475.4	0.43	terpene	aspterric acid	Aspergillus terreus NIH2624
BGC0001289.5	0.35	ribosomal	penisin	Paenibacillus ehimensis
BGC0001647.3	0.34	PKS	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0002254.3	0.33	PKS	notoamide F, (+)-semivioxanthin, waikikiamide B, waikikiamide C, notoamide A, waikikiamide A	Aspergillus sp. FM242
BGC0001964.2	0.33	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001928.2	0.32	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0001663.3	0.32	PKS	merocyclophane C, merocyclophane D	Nostoc sp. UIC 10110

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.98 out of 20.97

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 17.75 out of 18.57

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.38 out of 26.06

Confidence: medium

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 22.73 out of 29.25

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 27.26 out of 29.38

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.84 out of 29.38

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.26 out of 27.78

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.67 out of 21.55

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.39 out of 27.78

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.80 out of 26.33

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.60 out of 26.06

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 25.16 out of 28.19

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.73 out of 28.19

Confidence: strong

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.10 out of 32.93

Confidence: medium

Additional weak hits (11):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.42 out of 28.14

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.07 out of 30.22

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.45 out of 29.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.23 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.58 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 26.01 out of 32.74

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 22.59 out of 30.97

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.18 out of 25.75

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.96 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.01 out of 24.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.60 out of 26.06

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
ArcA	Repressor of carbon oxidation
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 1	Ferric Uptake Regulator
LexA variant 3	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
SypG	Regulator of biofilm formation and host colonization
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NZ_CP039297.1 - Region 10 - transAT-PKS

Download region GenBank file

Download region SVG

Location: 2,298,677 - 2,404,862 nt. (total: 106,186 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

E4T61_RS11265: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

E4T61_RS11345: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS11370: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

E4T61_RS11375: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

E4T61_RS11385: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS11390: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS11405: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS11410: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS11460: NADH:flavin oxidoreductase/NADH oxidase

smcogs: SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase

E4T61_RS11485: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

Transferases: acylase transferase

E4T61_RS11430: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

E4T61_RS11510: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: deacetylase hydrolase

E4T61_RS11350: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

E4T61_RS11505: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Lyases: hydratase

E4T61_RS11360: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

E4T61_RS11365: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

E4T61_RS11380: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E4T61_RS11395: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

E4T61_RS11415: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000176.5	0.93	PKS	difficidin	Bacillus velezensis FZB42
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0000174.5	0.60	PKS	bryostatin	Candidatus Endobugula sertula
BGC0002056.3	0.58	NRPS, PKS	peloruside A	sponge metagenome
BGC0001795.3	0.56	PKS	swinholide A	Nostoc sp. UHCC 0450
BGC0000067.5	0.54	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001012.3	0.53	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0000205.3	0.52	PKS	bryostatin	Candidatus Endobugula sertula

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000176.5	0.93	PKS	difficidin	Bacillus velezensis FZB42
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0000174.5	0.60	PKS	bryostatin	Candidatus Endobugula sertula
BGC0002056.3	0.58	NRPS, PKS	peloruside A	sponge metagenome
BGC0001795.3	0.56	PKS	swinholide A	Nostoc sp. UHCC 0450
BGC0000067.5	0.54	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001012.3	0.53	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0000205.3	0.52	PKS	bryostatin	Candidatus Endobugula sertula

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 17.22 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.51 out of 23.79

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.89 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.19 out of 27.78

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.44 out of 30.25

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.92 out of 27.78

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 22.84 out of 24.78

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.30 out of 25.31

Confidence: medium

Additional weak hits (8):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 26.71 out of 30.97

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 22.13 out of 30.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.26 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.34 out of 26.06

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.13 out of 25.75

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 21.46 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.61 out of 30.25

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.89 out of 32.93

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 12, location 2298676 - 2404862:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - ccmal - mal) + (ohmal - mal) + (mal) + (ccmal - ccmal - ohmal - ccmal) + (ohmal - mal) + (Me-ccmal) + (mal)
Non-colinear ordering:: E4T61_RS11405 + E4T61_RS11400 + E4T61_RS11395 + E4T61_RS11390 + E4T61_RS11385 + E4T61_RS11380 + E4T61_RS11375

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

E4T61_RS11415: X

AMP-binding (15..363): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ALFLHIGGINTAPATYGTEKQTYGLTELGIFSTK
Nearest Stachelhaus code(s):: 2,3-dohHda IGNPTGTIMK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

E4T61_RS11430: mal

PKS_AT (4..281): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 85.6
inactive: 30.4
Methoxymalonyl-CoA: 28.6
Methylmalonyl-CoA: 20.6
2-Methylbutyryl-CoA: 15.4
Propionyl-CoA: 14.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator
IolR	Regulator of the inositol catabolism
LexA variant 2	Repressor of DNA damage response
ToxT	Regulator of MSHA biosynthesis

NZ_CP039297.1 - Region 11 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 2,428,193 - 2,449,083 nt. (total: 20,891 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: carboxylase

E4T61_RS11740: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Ligases: synthase synthetase

E4T61_RS11705: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

E4T61_RS11710: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0002284.3	0.38	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis
BGC0000640.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC404

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0002284.3	0.38	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis
BGC0000640.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC404

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

E4T61_RS11710: PT_FPPS_like

Domain 1 (33 - 296): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 23.81 out of 29.25

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.14 out of 27.78

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.59 out of 30.25

Confidence: medium

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator
SypG	Regulator of biofilm formation and host colonization

NZ_CP039297.1 - Region 12 - NRP-metallophore,NRPS,RiPP-like,terpene-precursor

Download region GenBank file

Download region SVG

Location: 3,018,096 - 3,083,411 nt. (total: 65,316 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase reductase

E4T61_RS14965: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS15040: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS15090: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

E4T61_RS15100: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

E4T61_RS15145: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: esterase hydrolase

E4T61_RS14880: thioesterase superfamily protein

smcogs: SMCOG1144: thioesterase superfamily protein

E4T61_RS14930: isochorismatase

smcogs: SMCOG1027: isochorismatase

E4T61_RS15025: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: isomerase

E4T61_RS15035: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthetase

E4T61_RS14900: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

E4T61_RS15020: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

E4T61_RS15030: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000616.4	2.61	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0001185.5	1.68	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000309.5	1.07	NRPS	bacillibactin	Bacillus subtilis subsp. subtilis str. 168
BGC0000401.5	1.04	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002075.3	0.87	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000343.5	0.86	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.75	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000616.4	0.97	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0001185.5	0.94	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000309.5	0.81	NRPS	bacillibactin	Bacillus subtilis subsp. subtilis str. 168
BGC0000401.5	0.80	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000343.5	0.74	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

E4T61_RS14900: PT_FPPS_like

Domain 1 (56 - 285): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.04 out of 18.57

Confidence: strong

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 22.57 out of 27.55

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.00 out of 26.06

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.28 out of 23.79

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 29.38 out of 29.38

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 26.61 out of 29.38

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 27.15 out of 29.38

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.87 out of 27.78

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 26.49 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.91 out of 29.38

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.52 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.11 out of 26.06

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.97 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.08 out of 26.06

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.73 out of 28.19

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.98 out of 28.19

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.18 out of 28.19

Confidence: medium

Additional weak hits (19):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.88 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.74 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.56 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.45 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.36 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 22.44 out of 31.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 20.84 out of 29.25

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.61 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.80 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.80 out of 28.14

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.52 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.31 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.58 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 21.58 out of 26.12

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.44 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.15 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.85 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 23.99 out of 33.14

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 21.63 out of 30.12

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Terpene

TFBS Finder

Predicted core structure(s)

For candidate cluster 14, location 3018095 - 3083411:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 16, location 3031621 - 3083357:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

E4T61_RS15020: Gly - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (456..855): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDISGLELQALVGGETNLYGPTETTIWSAA
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1513..1929): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSTHFDFSVWEGNQIFGGEVNMYGITETTVHVSY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

E4T61_RS15030: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SISLYPLSSPGGALQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVVNK (100% 8Å match); diOH-Bz or Sal PLPAQGVVNK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 1	Ferric Uptake Regulator
GnfM	Regulator of nitrogen-fixation genes
IolR	Regulator of the inositol catabolism
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NZ_CP039297.1 - Region 13 - other

Download region GenBank file

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Location: 3,607,953 - 3,649,371 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

E4T61_RS18025: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS18045: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

E4T61_RS18080: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: aminotransferase transferase

E4T61_RS18030: aminotransferase

smcogs: SMCOG1019: aminotransferase

E4T61_RS18110: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

E4T61_RS18135: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Lyases: dehydratase lyase

E4T61_RS18040: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

E4T61_RS18100: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

E4T61_RS18105: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Translocases: permease

E4T61_RS17935: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

E4T61_RS18070: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001184.4	0.99	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000888.5	0.96	other (other)	bacilysin	Bacillus sp. CS93
BGC0000898.5	0.56	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000773.3	0.55	saccharide	lipopolysaccharide	Escherichia coli
BGC0000790.3	0.54	saccharide	O-antigen	Escherichia coli
BGC0000794.5	0.53	saccharide	S-layer glycan	Geobacillus stearothermophilus
BGC0000791.3	0.53	saccharide	O-antigen	Cronobacter sakazakii
BGC0000795.3	0.52	saccharide	S-layer glycan	Aneurinibacillus thermoaerophilus
BGC0001817.5	0.52	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000899.4	0.52	saccharide	desosamine	Streptomyces narbonensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001184.4	0.99	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000888.5	0.96	other (other)	bacilysin	Bacillus sp. CS93
BGC0000898.5	0.56	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000773.3	0.55	saccharide	lipopolysaccharide	Escherichia coli
BGC0000790.3	0.54	saccharide	O-antigen	Escherichia coli
BGC0000794.5	0.53	saccharide	S-layer glycan	Geobacillus stearothermophilus
BGC0000791.3	0.53	saccharide	O-antigen	Cronobacter sakazakii
BGC0000795.3	0.52	saccharide	S-layer glycan	Aneurinibacillus thermoaerophilus
BGC0001817.5	0.52	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000899.4	0.52	saccharide	desosamine	Streptomyces narbonensis

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.04 out of 18.57

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.85 out of 27.78

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.03 out of 26.85

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.63 out of 27.78

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.12 out of 23.79

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.75 out of 29.38

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.12 out of 28.19

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.47 out of 28.19

Confidence: medium

Additional weak hits (7):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 25.29 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 26.15 out of 36.54

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.45 out of 30.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 21.66 out of 30.97

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.62 out of 32.93

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.37 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.32 out of 26.06

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 1	Ferric Uptake Regulator
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence
RutR	Regulator of the pyrimidine and purine metabolism
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor