LOCUS       NZ_ML974128            11821 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Halorussus salinus strain YJ-37-H Scaffold2, whole genome shotgun
            sequence.
ACCESSION   NZ_ML974128
VERSION     NZ_ML974128.1
KEYWORDS    WGS; RefSeq.
SOURCE      Halorussus salinus
  ORGANISM  Halorussus salinus
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Halobacteria; Halobacteriales; Haladaptataceae; Halorussus.
REFERENCE   1  (bases 1 to 745088)
  AUTHORS   Cui,H.-L. and Han,D.
  TITLE     Genome sequence of Halorussus salinus YJ-37-H isolated from a marine
            solar saltern
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 745088)
  AUTHORS   Cui,H.-L. and Han,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2019) School of Food & Biological Engineering,
            Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang,
            Jiangsu 212013, China
REFERENCE   3  (bases 1 to 745088)
  AUTHORS   Cui,H.-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2019) School of Food & Biological Engineering,
            Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang,
            Jiangsu 212013, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2019
            Assembly Method                   :: SOAPdenovo v. Apr-2019
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004765815.2-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 11:43:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,705
            Genes (coding)                    :: 4,661
            CDSs (with protein)               :: 4,661
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 8 of 44
            Pseudo Genes (incomplete)         :: 39 of 44
            Pseudo Genes (internal stop)      :: 6 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:04:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 355326
            Orig. end                         :: 367147
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ML974128.1.
            Bacteria and source DNA available from School of Food & Biological
            Engineering, Jiangsu University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11821
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11821
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11821
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6821](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6821
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(175..1422)
                     /gene="glyA"
                     /locus_tag="EPL00_RS09880"
     CDS             complement(175..1422)
                     /EC_number="2.1.2.1"
                     /GO_function="GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0070905 - serine binding [Evidence IEA]"
                     /GO_process="GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (61-357). E-value:
                     1.2e-12. Score: 39.5. Matches aSDomain:
                     nrpspksdomains_EPL00_RS09880_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="glyA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325146.1"
                     /locus_tag="EPL00_RS09880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydroxymethyltransferase"
                     /protein_id="WP_135852865.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.4e-11, bitscore:
                     39.7, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.3e-07,
                     bitscore: 26.8, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYDRVRDVDPEVADALEAEVDRQRDTLEMIASENHVSEAVMEAQ
                     GSVLTNKYAEGYPGSRYYGGCEHVDTVEELAIERAKELWGAEHVNVQPHSGTQANMAVY
                     FAMLDPGDKILSLDLTHGGHLSHGHHANFTGQLYEVEQYEVDPETGYLDYEELRAHAEE
                     FDPDIVVSGYSAYPREVEWETIQEAADAVDAYHLADIAHITGLVAAGEHPSPVGVADFV
                     TGSTHKTIRAGRGGMVLCDDEYADDIDSAVIPGMQGGPLMHNIAGKAVGFREALQPEFE
                     EYAAQTVANAKALGESLQEHGFGLVSEGTDTHLVLVDLRESHEDVTGKDAEEALEDVGI
                     VLNANTVPGETRSPFVTSGIRAGTPALTTRGFDEEDCRYVGDLIARVVNNVEDDDVMAE
                     VAEEVQELCEANPIYE"
     aSDomain        complement(352..1239)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EPL00_RS09880_Aminotran_1_2.1"
                     /evalue="1.20E-12"
                     /label="EPL00_RS09880_Aminotran_1_2.1"
                     /locus_tag="EPL00_RS09880"
                     /protein_end="357"
                     /protein_start="61"
                     /score="39.5"
                     /tool="antismash"
                     /translation="YYGGCEHVDTVEELAIERAKELWGAEHVNVQPHSGTQANMAVYFA
                     MLDPGDKILSLDLTHGGHLSHGHHANFTGQLYEVEQYEVDPETGYLDYEELRAHAEEFD
                     PDIVVSGYSAYPREVEWETIQEAADAVDAYHLADIAHITGLVAAGEHPSPVGVADFVTG
                     STHKTIRAGRGGMVLCDDEYADDIDSAVIPGMQGGPLMHNIAGKAVGFREALQPEFEEY
                     AAQTVANAKALGESLQEHGFGLVSEGTDTHLVLVDLRESHEDVTGKDAEEALEDVGIVL
                     NANTVPGETRSPFVT"
     gene            1526..3634
                     /locus_tag="EPL00_RS09885"
     CDS             1526..3634
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977750.1"
                     /locus_tag="EPL00_RS09885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_135852864.1"
                     /transl_table=11
                     /translation="MIEWEETDSGLRVFDRTKTEVCIETTGWDAASAGYDVDRPLDDTI
                     SGYVSELRLPPALVKVVGLDSGEEYTHGGDADPLSLPDGEYLFNISLNIKTYLRFSGAA
                     TISKTDDYNELHISFPEPTLVTFGFRSHHEEPVDTITVPPTPDGVATAVTYLSSSHKVT
                     GPDRSYPTLRGHPPRIELGEKTEIPEPVREERFDVGIELVVPDEFAYTFVAAPLAYYLQ
                     AKVIVEDRRSPVLRAPETGVEHRFTPLPGFQHEVADTLRRVFFLDCLVRNAGEYSWDLA
                     ELPLLDEVGIEAEETYDSSPAEQLATYLDAPYERIDDDLPEWHLAMHVEPLSDHVTSLP
                     YFLDNLSLVYLPESTDLEKDELLNKSIDDFYRAGKPASPRSNLGLTKTTARMTRKGPGP
                     VKSVNRRDPTLHEGKVNGWLADGVPIDVFKAVPEAYRNRFKYLDTDGGNIDVMVILNDE
                     EMADEHETAAEIYKNRAQELPIDVTVLERCSKDELAEVFETPHDFVHYIGHCENDGLRC
                     RNGNLSVSDIQESNVQTFFLNACGSYYEGQDLVKKGSVAGAVTFTKVLNKQAAKVGVAF
                     AQLLINGYDIAHAIQLARRRIMMGKDYAVVGDGTHQLTQTDNRIPTLGRLTETDEMYNA
                     KFNSLTVDHIGGCYEVYLPNSDCLQLHGNESTVEISKSELSSFLDRAEMPFIYDGDLYW
                     SDELAERL"
     misc_feature    1685..1687
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    2174..2176
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    2186..2188
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4096..4920
                     /gene="tbsP"
                     /locus_tag="EPL00_RS09890"
     CDS             4096..4920
                     /codon_start=1
                     /gene="tbsP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977749.1"
                     /locus_tag="EPL00_RS09890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transcriptional regulator TbsP"
                     /protein_id="WP_135852863.1"
                     /transl_table=11
                     /translation="METESNLLDQEIAEILRTVIDEAPESMLVVNPSKNAIEELITVAT
                     EYEGDLPELRMLAESGTLKDVMEDFIVGSNAADLIDAGSLSLRTTDDVPSNSLLVTDEV
                     VIALVTADDRVGGLTADDEEFVAEAYDSYSAQFEAADEFSLRTPPLSRVRDTLGEEISP
                     DAQNDFDAVLSSMETARGDGDGLDEVTISLLVAAKNEALLYDISKWGEDVGIASKATFS
                     RTKTKLEDMGLIGTEKVPIDVGRPRLRLKLANDELRNADTDQLASAAQSLLH"
     misc_feature    4114..4116
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..6821
                     /locus_tag="EPL00_RS09895"
     CDS             5001..6821
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741151.1"
                     /locus_tag="EPL00_RS09895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_135852862.1"
                     /sec_met_domain="YcaO (E-value: 5.2e-65, bitscore: 215.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 3.4e-75, bitscore:
                     248.7, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRPTVGLVGEGPAIDAVAAALADVDAETVECAPEQVAGTDFAVV
                     AGDADDSRAPDDRHPTRFAAANRAAREGGTPWLAVELGGVGGRALDIDAAVSGFAPGTA
                     CWQCLRTRVAANADESEASSGTDPDPDPDPAVARFAGALAGREAATLLSGGESRILGGV
                     VEVPHAERNLLPVPGCEVCDDDSDVSLRLSASLDREYADTELEGALSRAEQAMDERVGL
                     VSTIGEAESFPAPYYLAQLADTSGFSDAQAAEQAAGVADDWNAALMKALGEALERYAAG
                     VYREEGFRTARTGDLDAADSDGDGSDSVVAPTQFVRPEEWTGTDADTELSWVEGRNLHS
                     DEQVHLPAEFVHFPPPEVRHKPSITTGLGLGSSTVEALLAGCYETIERDATMLSWYSTF
                     DPLELAVEDERFDALRARAHAEDLEVTPLLVTQDVDVPVVAVAVHREGEWPRFAVGSDA
                     DLDPEEAATSALAEALQNWMELRSMGEDDAADADGAIGRYADFPATAREFVASETAIPA
                     ASVGPADPPTGEAELDALLERLADADLSAYAARLTTRDVERLGFEAVRVLVPAAQPLFT
                     GEAFFGERSREVPPELGFEFRPDRDLHPYP"
     gene            6945..7889
                     /locus_tag="EPL00_RS09900"
     CDS             6945..7889
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008010783.1"
                     /locus_tag="EPL00_RS09900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_135852861.1"
                     /transl_table=11
                     /translation="MYAPTRLAGYVVLLLTPVALLASALFSGGVSAAVLGLEGGVALAG
                     GVAAASERERALEAPDAHDLDAGDLADAFAVLAAAVVTYVLSVRFGLGPVVASALVGLV
                     AGVGTPRIAVPAYCGSFVGMASPALFPSVGYLALAGLVSGVAFVAAERAFDGFGGKLGT
                     LALFGCAATAALTGADFTAGSALAWTDALVVVPVSVAGAVGTVVLSLRLDLGAVVGSAV
                     VGLAAGLGLPAMSGASGETLAAAAFCASFVGMSSTDRLDGEERVALAGAVCGLVFVAVA
                     AAFSGAGGKLGTIAFVSCVTTAGGERLVVRMRS"
     gene            8015..8212
                     /locus_tag="EPL00_RS09905"
     CDS             8015..8212
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EPL00_RS09905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="WP_135852860.1"
                     /transl_table=11
                     /translation="MTSEYELDCATCGTSLTRREVPGESLGFGGSDAPELGDSESLEVA
                     ECPDCGGRYFPETTLERLET"
     gene            complement(8228..9355)
                     /locus_tag="EPL00_RS09910"
     CDS             complement(8228..9355)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006432643.1"
                     /locus_tag="EPL00_RS09910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF63 family protein"
                     /protein_id="WP_135852859.1"
                     /transl_table=11
                     /translation="MSTVTEGMDSERLWGGTVAALLAALVGGALVFPERVYYGFVWHYF
                     WGPVVADAKSAQCAAYADGSVSFLYESSACAAAAEPVAYPGYTLVSEVGYALTMLLALL
                     GVVFMLRRLGVGQDRELFYALFPYMLFGGALRVVEDAFDGLDPSQAAITFPWNTLIISP
                     VIYFTMFAITLAALLASIWLRNSGATEGYRYTVAGIGTALLSVTVGYLGFLAFTTDYVD
                     FFPQITVVVLVSATLVTWLTWEGLTRYAPEVNEGTGKMGIVVLWAHSVDGVANVVGIDW
                     AKELGLPADLVPKHPVNRALIEVGKQFPEPVVDLIGTAWPFLLVKIAAAIFVVWVFDEG
                     IFEESPRYAILLLVAIVAVGLGPGTRDMLRATFGI"
     gene            complement(9493..9957)
                     /locus_tag="EPL00_RS09915"
     CDS             complement(9493..9957)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977744.1"
                     /locus_tag="EPL00_RS09915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_135852858.1"
                     /transl_table=11
                     /translation="MELDDTDREILEALQENARTPFSEIARRIDMSSATVHDRVNRMEE
                     AGVIEGYHAKVDPKALDYGISAVVGLQVEQGQEQNTLGRLEDIDGIQEVHLTTGSWDVL
                     MRVYAEDADRLRELMFENVAQMDGFARSQTMVILGTPYETEELPIDGSEV"
     misc_feature    complement(9916..9918)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9925..9927)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(10051..10845)
                     /locus_tag="EPL00_RS09920"
     CDS             complement(10051..10845)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1207: inositol monophosphatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009367436.1"
                     /locus_tag="EPL00_RS09920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="inositol monophosphatase"
                     /protein_id="WP_135852857.1"
                     /transl_table=11
                     /translation="MTDDADDLLAVAKRAASAGSEVAAESFRAGIEVETKSDKTDVVTE
                     ADRQTQRRVIEVVREEYPDDAIVGEEEDELKEVPDEGDAWVIDPIDGTNNYVRSIPVWT
                     TSVAAVRDGEPVAAVNDCPALGETYVAGRDGAYRDGEPVSVSEKTDPETFAVVPTIWWG
                     FDRRDEYAAVCEELVERFADVRRFGSAQVTLGMVAAGSLEGAVTNVDPNPWDTVAGVHM
                     VRQAGGTVTDRHGDPWRHDSESLVASNGEAHDELVAALRNVA"
     tRNA            11139..11223
                     /anticodon=(pos:366501..366503,aa:Leu,seq:caa)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="EPL00_RS09925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Leu"
     gene            11139..11223
                     /locus_tag="EPL00_RS09925"
     gene            11460..11720
                     /locus_tag="EPL00_RS09930"
     CDS             11460..11720
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014556145.1"
                     /locus_tag="EPL00_RS09930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_135852856.1"
                     /transl_table=11
                     /translation="MQWHRRRDLEGGKELGVWLLVADDGSVERELYVESHEYRGGDFDV
                     YTTADDEWTHEGEFETSEEAFARAQALLESSSHAVESDGLA"
ORIGIN
        1 gcgtcgctcg gcgcatcgcc gccgaccgac gagagacaac cggagagcga cgcgacgatg
       61 cccgcgctcg cggccagcag atggcgacgg gaccgattca tggaaaaacg attttctgtc
      121 gtcgaaataa gctttctgtc cgcgaaagaa ggccgtaacg ggagtaacga gaccttactc
      181 gtaaatcggg ttcgcctcgc agagttcctg aacctcctcc gcgacttcgg ccatcacgtc
      241 gtcgtcctcg acgttgttca cgacgcgcgc gatgaggtcg ccgacgtacc ggcagtcctc
      301 ctcgtcaaag ccccgagtcg tcaaggcggg cgtccccgcg cggatgcccg aggtgacgaa
      361 cggcgagcgc gtctcgcccg gcacggtgtt tgcgttcagc acgatgccaa cgtcttcgag
      421 ggcctcctcg gcgtccttgc cggtcacgtc ctcgtgggac tcccggaggt ccaccagcac
      481 gaggtgggtg tcggtcccct cggagacgag gccgaacccg tgttcttgca ggctctcgcc
      541 gagcgccttc gcgttggcga cggtctgggc ggcgtactcc tcgaactcgg gctggagagc
      601 ttcccggaag ccgaccgcct tgcccgcgat gttgtgcatc aggggaccgc cctgcatccc
      661 cggaatcacc gcggagtcga tgtcgtcggc gtactcgtcg tcgcacagca ccatcccgcc
      721 gcgacccgcg cggatggtct tgtgcgtcga accggtcacg aagtcggcga cgccgaccgg
      781 cgaggggtgt tcgcccgcgg cgacgagtcc cgtgatgtgg gcgatgtccg cgaggtggta
      841 ggcgtccacc gcgtcggcgg cctcctgaat cgtctcccac tcgacctctc gggggtacgc
      901 cgagtaaccc gagaccacga tgtccgggtc gaactcctcg gcgtgagcgc gaagctcctc
      961 gtagtcgaga taccccgtct cggggtccac ctcgtactgc tcgacctcgt acaattggcc
     1021 ggtgaagttg gcgtggtggc cgtggctgag gtggccgccg tgggtcaggt cgagcgagag
     1081 aatcttgtcg ccggggtcga gcatggcgaa gtagaccgcc atgttcgcct gcgtccccga
     1141 gtggggttgg acgttgacgt gttcggcacc ccacagctcc ttcgcgcgtt cgatggctag
     1201 ctcctcgacg gtatcgacgt gttcgcatcc gccgtagtag cgcgaaccgg ggtagccctc
     1261 ggcgtacttg ttggtgagaa cgctcccttg ggcctccatc actgcctcgc tgacgtggtt
     1321 ctcgctggcg atcatctcca aggtgtcgcg ctggcggtct acctcggctt cgagtgcgtc
     1381 tgctacctcc gggtcaacgt cgcggacgcg gtcgtactcc atatcaaaaa tcgcggcatc
     1441 ccggagtata agcttacctt tcgcgggggc aaccacgggc gacaaaaata taaagtcccg
     1501 gtgagacacg ttgtacgacg ccgacatgat tgagtgggag gagacggatt ccgggttgcg
     1561 cgtgttcgac cgcacgaaga cggaggtgtg tatcgagacg accggttggg acgcggcgag
     1621 cgcgggctac gacgtggacc gcccactcga cgataccatt tccggatacg tctcggaact
     1681 tcgcttaccg ccagcgctgg tgaaggtcgt cggtctcgac tccggcgagg agtacaccca
     1741 cggcggtgac gccgacccgc tctctctccc cgacggtgag tatctgttca atattagtct
     1801 caatatcaaa acatacctgc ggttttccgg ggccgcgaca atttcgaaga cggacgacta
     1861 caacgaactc cacatatcgt ttccggaacc gacgctcgta accttcggat tccggagcca
     1921 ccacgaggag cctgtcgata cgattaccgt cccgccgacg cccgacggcg tggcgactgc
     1981 cgtcacgtat ctctcgtcgt cacacaaggt tacaggtccc gaccggtcgt acccgacgct
     2041 tcgaggtcac ccaccccgaa tcgaactcgg tgagaagacg gagattccgg agccggtccg
     2101 tgaggagcga ttcgacgtgg gtatcgagct agtggttccc gacgagttcg cgtacacgtt
     2161 cgtcgcagca ccgttagcgt actatttaca agccaaggtg atcgtcgagg accgtcggtc
     2221 gccggtcctt cgcgcgcccg agaccggcgt cgaacatcgg ttcacgccgt tgcccggctt
     2281 ccagcacgaa gtcgccgata cgcttcggcg cgtgttcttc ctcgattgcc tcgtcagaaa
     2341 cgcgggtgag tatagttggg acctcgcgga actcccactg ttggacgagg tgggtatcga
     2401 agccgaggag acctacgact cctcgcccgc cgaacaactc gccacctacc tcgacgcgcc
     2461 gtacgagcgt atcgacgatg acttgccgga gtggcacctc gccatgcatg tcgaacccct
     2521 ttcggatcac gtaacgtcgc ttccgtattt cctcgacaac ctcagcctcg tctacctacc
     2581 cgagtcaacc gacttggaga aggatgaact gctcaacaag tctatcgatg acttctaccg
     2641 tgcgggtaag ccagcgtctc ctcgatcgaa tctagggctt acgaaaacaa ctgctagaat
     2701 gactcgtaaa ggtcccggtc cggttaagtc ggtaaaccgg cgtgacccga cgctccacga
     2761 agggaaagta aacggatggc ttgccgacgg tgttccaatc gacgttttca aagcggttcc
     2821 cgaagcgtac cggaaccggt tcaaatatct tgacacagac ggcggaaaca tcgacgtgat
     2881 ggtcattctc aatgatgaag agatggccga cgaacacgag acggccgctg aaatatacaa
     2941 aaaccgggca caggagcttc caatagacgt taccgtcctc gaacgttgct ccaaagacga
     3001 actcgccgaa gtgttcgaga ctccacacga ctttgtccac tatatcggcc actgtgagaa
     3061 cgacggtctt cgatgtcgaa acggcaatct gtcggtttcg gacatccaag agagcaacgt
     3121 ccagacgttc ttcttgaacg cctgtggctc ctactacgaa gggcaagact tggtgaagaa
     3181 agggagcgtc gccggtgcgg tgacgttcac gaaggtgctg aacaaacaag cggcgaaagt
     3241 cggtgtggcg ttcgcccaac tgctcatcaa cggatacgac attgcacacg cgatacaact
     3301 cgcccgccgc cgtatcatga tggggaaaga ttacgcagta gtcggggatg gaacccacca
     3361 acttacccag acggataacc gcatcccgac gctcggacga ctcaccgaga ccgatgagat
     3421 gtacaacgcg aagttcaact ctcttacagt tgatcacatc ggcggttgtt atgaggtcta
     3481 ccttcctaat tcggactgcc ttcagctcca cggcaacgaa tctactgtcg aaatatcgaa
     3541 atccgaacta tcttcgtttc tcgaccgcgc agaaatgccg tttatttatg atggtgatct
     3601 gtactggtcc gatgagttgg cagagcgcct ataagccggt ctacggtccc gccgtacttc
     3661 cagcgcctgc ctgcggcagt acttcaacaa gaatggaact catgaacacg aaagcgtacg
     3721 ctgcgtccat cgctcgcgga ttctccatga cccattgtcg tatcttccct tgcatggctt
     3781 gtttcgtgtt acagaacccc actactttat tgttttgtat atttcatacg ctagtagacg
     3841 gggaataacc caagaaaggg gccattaaac ctattcgtcg ccacggcgaa tccgagacga
     3901 ggtccggcga gcccggcgtc gccagtcgat tttcacgcga ggtattgttt aagattcaca
     3961 agggcgtaga acccaaaacc gactccgaaa ttcgtttaga attgtcgaac tgaccgctgg
     4021 aagtctccag ataatccgtg atttcccccc gttactaacg ttaaaattaa gtaggggtaa
     4081 aacgtttgtc ggtacatgga gaccgaatcg aatttactcg accaagaaat cgccgaaatc
     4141 cttcggacgg tcatcgacga agcgcccgaa tcgatgctcg tcgtcaaccc gtcgaagaac
     4201 gctatcgaag aactgatcac cgtcgccacg gagtacgagg gcgacctgcc ggaactccgc
     4261 atgctcgcgg agtcgggtac gctgaaagac gtgatggagg acttcatcgt cggcagtaac
     4321 gcggccgacc tcatcgacgc ggggtcgctc tcgcttcgca ccaccgacga cgtgccgagc
     4381 aactctctgc tcgtcaccga cgaagtcgtc atcgcgctcg tgaccgcgga cgaccgtgtg
     4441 ggcggcctca cggccgacga cgaggagttc gtcgcagaag cctacgactc gtactccgcg
     4501 cagttcgagg ccgccgacga gttctcgctg cgcacgccgc cgctctcgcg cgtccgcgac
     4561 acgctcggcg aggagatcag ccccgacgcc cagaacgact tcgacgcggt tctctcctcg
     4621 atggagaccg cgcgcggaga cggcgacgga ctcgacgagg ttaccatcag tctcctcgtc
     4681 gcggcgaaga acgaggccct gctgtacgac atcagcaagt ggggcgagga cgttggcatc
     4741 gccagcaagg cgacgttctc gcggacgaag acgaagctcg aagacatggg cctcatcggg
     4801 accgagaagg ttcccatcga cgtgggtcgc ccgcgcctgc gcctgaagtt ggccaacgac
     4861 gaactccgta atgcggacac cgaccagctc gccagcgcgg cccagagcct gctgcactga
     4921 ccacgaactc gcactttttc gcgcggtagc ccccaagcga tagcaccgcg tttttccggt
     4981 ctccgttcct cgatgtcgtc atgaaccgac cgacagtcgg actcgtcggc gagggtccgg
     5041 caatcgacgc ggtcgcggcc gcgctcgccg acgtggacgc cgagaccgtc gagtgtgccc
     5101 ccgaacaggt cgcagggacc gacttcgccg tcgtcgccgg tgacgccgac gactcccgcg
     5161 ctcccgacga ccgtcaccct acgcgcttcg ccgcggcgaa ccgcgcggcc cgcgagggcg
     5221 ggacgccgtg gctcgcggtc gaactcggcg gcgtcggcgg tcgcgctctc gacatcgacg
     5281 ccgcggtctc gggattcgcg ccggggaccg cgtgctggca gtgtctccgg acgcgcgtcg
     5341 ccgcgaacgc agacgagagc gaagcgagtt ccggaaccga ccccgacccc gaccccgacc
     5401 cggccgtcgc gcgcttcgcg ggcgcgctcg ccgggcgtga ggccgccacc ctcctgtctg
     5461 gcggcgagtc gcggattctg ggcggcgtcg tggaagtgcc ccacgccgag cgaaacctgt
     5521 tgcccgtgcc cggctgtgag gtctgcgacg acgactccga cgtgagtctc cgtctctcgg
     5581 cgtcactcga ccgcgagtac gcagacaccg aactggaggg cgcactctcg cgcgccgagc
     5641 aggccatgga cgagcgcgtc ggtctcgtct cgacaatcgg cgaggccgag tcgttccccg
     5701 cgccctacta cctcgcccaa ctcgccgaca cctcggggtt cagcgacgca caggccgcag
     5761 aacaggccgc tggcgtcgcc gacgactgga acgccgcgct gatgaaggcg ctcggcgagg
     5821 cgctggaacg ctacgccgcg ggcgtctacc gcgaggaggg cttccggacg gcgcgcacgg
     5881 gcgacctcga cgcggccgat tccgacggag acggctccga ctcggtggtc gcgcccaccc
     5941 aattcgtccg gcccgaggag tggaccggca cggacgctga caccgaactg tcgtgggtcg
     6001 agggccggaa cctccacagc gacgagcagg tccacctccc ggcggagttc gtccacttcc
     6061 cgccgcccga ggtccgtcac aagccctcca tcacgaccgg tctcggtctc ggaagttcga
     6121 ccgtcgaagc cctcctcgcg ggctgttacg agaccatcga gcgcgacgcg acgatgctct
     6181 cgtggtactc gacgttcgac ccgctggaac tcgcggtcga ggacgagcgg ttcgacgcgt
     6241 tgcgggcgcg cgctcacgcg gaagatttgg aggtcacgcc cttgctggtc acgcaggacg
     6301 tggacgtgcc ggtggttgcg gtcgcggtcc accgcgaggg cgagtggccc cggttcgcgg
     6361 tcggctccga cgccgacctc gaccccgagg aggccgccac gtccgcgctc gccgaggcgc
     6421 tccagaactg gatggaactg cgttcgatgg gcgaggacga cgccgccgac gccgacggtg
     6481 ccatcggtcg gtacgccgac ttccccgcga ccgcccgcga gttcgtggcg agcgagaccg
     6541 cgattcccgc cgcgagcgtc ggtccggccg acccgccgac cggggaggcg gaactcgacg
     6601 cgctcctcga acgcctcgcc gacgccgacc tgtcggccta cgccgcgcgc ctgacgaccc
     6661 gcgacgtgga gcgactcgga ttcgaggcgg tccgggtact ggtccccgcg gcccaaccgc
     6721 tgttcaccgg cgaagcgttc ttcggcgagc ggtcgcgcga ggtgcccccg gaacttggct
     6781 tcgagttccg gcccgaccgc gacctgcatc cgtacccctg agcgtcgggc ggacggcgct
     6841 cctcgcggaa atcgtcccgc gacaatcgtc ccgcggaaat tgtcccgcgg caatcgaccc
     6901 cggaatcgca acccactaca ggggtcgccg gactacggat tggcgtgtac gcgccgactc
     6961 gcctcgcggg ctacgtcgtc ctgttgctca cgccggtcgc gctactcgcc agcgcgctgt
     7021 tctccggcgg cgtctcggct gcggtcttgg gtctcgaagg aggagtcgcc ctcgctggcg
     7081 gggtcgccgc cgcgagcgaa cgggagcgcg cgctcgaagc gcccgacgcc cacgacctcg
     7141 acgccgggga cctcgcggac gcgttcgcgg ttctcgccgc ggcggtcgtg acctacgtcc
     7201 tgagcgtccg gttcggtctc ggcccggtcg tcgcctccgc gctcgtcggt ctcgtcgcgg
     7261 gagtcggaac tcctcgaatc gcagttccgg cctactgcgg gtcgttcgtc ggcatggcct
     7321 cgcccgccct cttcccgtcg gtcggctacc tcgcgctcgc gggtctcgtc tcgggcgtcg
     7381 cgttcgtcgc ggccgagcgc gccttcgacg ggttcggcgg gaagttgggg acgctcgcgc
     7441 tgttcgggtg cgcggcgacg gccgcgctga ccggcgcgga cttcaccgcc gggagcgcgc
     7501 tcgcgtggac cgacgcgctc gtcgtcgtcc cggtttcggt cgcgggtgcc gtcgggaccg
     7561 tcgtcctgag cctccgcctc gacctcgggg cggtcgtcgg gtccgcggtc gtcggtctcg
     7621 cggcgggtct cggtctcccg gcgatgtcgg gcgcgtcggg cgagacgctg gccgcggcgg
     7681 cgttctgcgc ctcgttcgtc ggcatgagtt cgaccgaccg actcgacggc gaggagcgcg
     7741 tcgcgctcgc cggggcggtc tgcggactgg tgttcgtcgc ggtcgcggcg gcgttctcgg
     7801 gcgcgggcgg gaagttgggg actatcgcgt tcgtctcctg cgtcacgacc gccggtgggg
     7861 agcgactggt cgtccgaatg cggtcgtgag tgcgctccgc cggaccgggc gacatcgtcg
     7921 agagctttct cagacaaggg ttttatctcc cgccccgtag tgtgtggtac cgtatgtcgc
     7981 cattctcacc gaccgagtat ccggggggac ggggatgact tccgagtacg aactcgactg
     8041 cgcgacctgc ggcacgtcgc tgactcgccg cgaggttccc ggcgagtcgc tgggattcgg
     8101 ggggtccgac gcgccggaac tcggcgactc ggagtcgctg gaggtcgcgg agtgtcccga
     8161 ctgcggcggc cgctacttcc cggagacgac cttggaacga ctcgaaacct gaaaaatcga
     8221 ctcgctttta gatgccgaac gtcgcgcgga gcatgtcccg cgtgccggga ccgagaccga
     8281 cggcgacgat ggccacgagg agcaggatgg cgtagcgcgg gctctcctcg aagatgccct
     8341 cgtcgaacac ccagacgacg aagatggcgg ccgcgatttt gaccagcagg aagggccacg
     8401 cggtgccgat gaggtccacg actggttcgg ggaactgctt gccgacctcg atgagcgcgc
     8461 ggttgacggg gtgtttcggc acgaggtcgg cgggcaggcc cagttccttc gcccagtcga
     8521 tgccgacgac gttggccacg ccgtcaaccg agtgcgccca gaggacgacg atacccatct
     8581 tgcccgtccc ctcgttgacc tcgggcgcgt agcgagtgag accttcccac gtcagccacg
     8641 tcacgagcgt cgcgctgacg aggacgacga ccgtaatctg ggggaagaag tccacgtagt
     8701 cggtcgtgaa cgcgaggaag ccgaggtagc cgaccgtcac cgagagcagg gccgtgccga
     8761 tgcccgcgac ggtgtagcgg tagccctcgg tcgcgccgga gttgcggagc cagatgctcg
     8821 ccagcagggc cgccagcgtg attgcgaaca tcgtgaagta gatgaccggg ctgatgatga
     8881 gggtgttcca cgggaacgtg atggcggcct gcgaggggtc gagtccgtcg aaggcgtcct
     8941 cgaccacgcg gagcgcgccg ccgaacagca tgtaggggaa cagcgcgtag aacagttcgc
     9001 ggtcctgtcc gaccccgaga cgacggagca tgaagacgac gcccagtagc gccaacagca
     9061 tcgtcagcgc gtagccgacc tccgagacca gcgtgtagcc ggggtacgcg accggttcgg
     9121 ccgcggccgc gcaggcgctc gactcgtaga ggaaactcac cgacccgtcg gcgtaggccg
     9181 cacactgggc gctcttggcg tccgcgacga ccggccccca gaagtagtgc cagacgaaac
     9241 cgtagtagac gcgctcggga aagacgagag cgccgccgac gagcgcggcc aacagcgcgg
     9301 cgaccgtccc accccagagg cgttcggagt ccattccctc ggttacggta ctcatatttg
     9361 ccaaggcgca agccgaccgt tttacggttc cggtctcggt cgtcgccgcc gctcgtcaca
     9421 gagtccattc tagtcggtgg tccggtcgaa gacgtgtccg gtcgcggaga acgctaggga
     9481 tgagcgccgg tctcagactt cgctaccgtc gatgggcaac tcctcggtct cgtagggcgt
     9541 accgaggatg accatcgtct gggagcgcgc gaagccgtcc atctgagcga cgttctcgaa
     9601 catcaactcg cgaagtcggt cggcgtcctc ggcgtagacg cgcatcaaca cgtcccacga
     9661 accggtcgtg aggtggacct cttggatgcc gtcgatgtct tcgaggcgac cgagggtgtt
     9721 ctgttcctgt ccctgttcga cctgtaggcc gacgaccgcc gagataccgt agtcgagtgc
     9781 cttggggtct accttcgcgt ggtacccctc gatgacgccc gcctcctcca tccggttgac
     9841 tctgtcgtgg accgtcgcac tggacatgtc gatacgacgc gcgatctcgc tgaagggggt
     9901 tcgcgcgttc tcctgtaacg cttctaagat ttcgcggtcg gtatcatcga gttccatatc
     9961 ctacatcaga ttctttcagg ctaaatatcc ttagaattca tctcgtcaga aacgggggat
    10021 tacgtcctcg gtggaacgac acaatacggt tcaggcgacg tttcgaaggg cggccaccag
    10081 ttcgtcgtgg gcctcgccgt tcgaggcgac gaggctctcg gagtcgtgtc gccacgggtc
    10141 gccgtgccgg tcggtgacgg tcccgcccgc ctgtcggacc atgtgaacgc cagccacggt
    10201 gtcccacggg ttcgggtcca cgttcgtcac cgcgccctcc agcgacccgg ccgcgaccat
    10261 cccgagcgtg acctgcgccg acccgaaccg gcgcacgtcg gcgaatcgct cgaccaactc
    10321 ctcgcagacc gcggcgtact cgtcgcgccg gtcgaacccc caccagatgg tcggtacgac
    10381 ggcgaacgtc tcggggtcgg tcttctcgct gaccgagacg ggttcgccgt cgcggtacgc
    10441 gccgtcgcgt ccggcgacgt acgtctcgcc gagcgcgggg cagtcgttga ccgcggcgac
    10501 cggttcgccg tcccggaccg ccgcgacgct ggtcgtccag accggaatcg accggacgta
    10561 gttgttcgtc ccgtcgatgg ggtctatcac ccacgcgtcg ccctcgtcgg ggacctcctt
    10621 gagttcgtcc tcctcctcgc cgacgattgc gtcgtcgggg tactcctcgc ggacgacttc
    10681 gatgactcgg cgctgagtct ggcggtcggc ctcggtcacc acgtcggtct tgtcggattt
    10741 ggtctcgact tcgatgcccg ctcgaaagct ctcggcggcg acttcgcttc ccgcgctcgc
    10801 ggcgcgcttc gccaccgcga ggaggtcgtc ggcgtcgtcg gtcatgtttc cgcggtgggg
    10861 cgtcgccgga aaagccctgt cggttcggcg gtttcggccg gtgcagtgcg gttgcggtac
    10921 gacgggagtc ggcgatactc cgctgtattg ttgcggtgcg gttccgttgg tgtcgggaag
    10981 agtcaccttc tctggattct cgtgtaggtc cgtgacgcgg accactcaag ccaccgcacc
    11041 gatgcccgcc ggtccccgga atccgacacc gtaggaggtt tatatagcgg ccgttttaga
    11101 gacgaacgtc aggcgcgaac cccagacgaa gacagcgtgc gagggtagcc aagcctggcc
    11161 aacggcggcg gactcaagat ccgctcccgt aggggtccaa gggttcgaat cccttccctc
    11221 gcactaccgg tgtggcgtcg ccacacccaa cagtgcaagc aagaccgctt cggagcgtct
    11281 tgccctcgca aactacacgg cccacgcggg ccctcgcaac attcctctgg tgccagtctc
    11341 gtagcgactg ctctcgcttg gtcgcaaccg tttccggttg gtcgcaaccg ctctcggttc
    11401 gtcgcagtgt gagttctcac gtcaagagct aactacatgc aaatcgtggg agcagagcga
    11461 tgcagtggca ccgacggcgc gacctcgaag gcggcaagga attgggcgtc tggttgctcg
    11521 tggccgacga cgggagcgtc gagcgagaac tctacgtcga gtcccacgag taccgcggcg
    11581 gggacttcga cgtgtacacg acggccgacg acgagtggac tcacgagggg gagttcgaga
    11641 ccagcgagga ggccttcgct cgcgcgcagg ccctcctcga atcgagttcc cacgcggtcg
    11701 agagcgacgg actcgcctga gaaacctact cgaaaagcac cagcgggtcg gcctcgcgcg
    11761 cggcgaaata ctcgtccaac tcgcgctttc gggcgggaat ctcgtcgggg tcgtgggcgt
    11821 c
//
