LOCUS       NZ_SBIT01000001        11758 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Halorussus ruber strain YC25 Scaffold1_1, whole genome shotgun
            sequence.
ACCESSION   NZ_SBIT01000001
VERSION     NZ_SBIT01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Halorussus ruber
  ORGANISM  Halorussus ruber
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Halobacteria; Halobacteriales; Haladaptataceae; Halorussus.
REFERENCE   1  (bases 1 to 510403)
  AUTHORS   Cui,H.-L.
  TITLE     Genome sequence of Halorussus ruber YC25, isolated from an inland
            salt lake of China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 510403)
  AUTHORS   Cui,H.-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2019) School of Food & Biological Engineering,
            Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang,
            Jiangsu 212013, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. Dec-2018
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004765785.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 04:20:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 4,270
            CDSs (with protein)               :: 4,270
            Genes (RNA)                       :: 60
            rRNAs                             :: 3, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 18 of 66
            Pseudo Genes (incomplete)         :: 50 of 66
            Pseudo Genes (internal stop)      :: 6 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:04:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 353545
            Orig. end                         :: 365303
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SBIT01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11758
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11758
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11758
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6758](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6758
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(161..1408)
                     /gene="glyA"
                     /locus_tag="EPL01_RS06340"
     CDS             complement(161..1408)
                     /EC_number="2.1.2.1"
                     /GO_function="GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0070905 - serine binding [Evidence IEA]"
                     /GO_process="GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (60-357). E-value:
                     1.2e-13. Score: 42.9. Matches aSDomain:
                     nrpspksdomains_EPL01_RS06340_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="glyA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325146.1"
                     /locus_tag="EPL01_RS06340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydroxymethyltransferase"
                     /protein_id="WP_135824409.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 9e-13, bitscore:
                     43.1, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.6e-05,
                     bitscore: 19.3, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYDRVRDSDPEVADALEAEVERQRDTLAMIASENHVSEAVMEAQ
                     GSVFTNKYAEGYPGSRYYGGCEHIDTVEELAIERAKELWGADHVNVQPHSGTQANMGVY
                     FAMLEPGDKILSLDLTHGGHLSHGHHANFTGELYEVEQYEVDPETGYLDYEAIQDHAEE
                     FDPDIIVSGYSAYPRAVEWETIQEAADAVDAYHLADMAHITGLVAAGEHPSPVGVADFV
                     TGSTHKTIRAGRGGMILCDEEYADAIDSAVIPGMQGGPLLHNIAGKAVGFGEALQPEFE
                     EYAAQTVKNAEALGESLQEHGFELVSGGTDTHLVLVDLRESHPDVTGKEAEEALEDVGI
                     VLNANTVPGETRSPFVTSGIRAGTPALTTRGFDEEDCRYVGDLIARVVNNVGDEDVKAE
                     VAEDVQELCDANPLYE"
     aSDomain        complement(338..1228)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EPL01_RS06340_Aminotran_1_2.1"
                     /evalue="1.20E-13"
                     /label="EPL01_RS06340_Aminotran_1_2.1"
                     /locus_tag="EPL01_RS06340"
                     /protein_end="357"
                     /protein_start="60"
                     /score="42.9"
                     /tool="antismash"
                     /translation="RYYGGCEHIDTVEELAIERAKELWGADHVNVQPHSGTQANMGVYF
                     AMLEPGDKILSLDLTHGGHLSHGHHANFTGELYEVEQYEVDPETGYLDYEAIQDHAEEF
                     DPDIIVSGYSAYPRAVEWETIQEAADAVDAYHLADMAHITGLVAAGEHPSPVGVADFVT
                     GSTHKTIRAGRGGMILCDEEYADAIDSAVIPGMQGGPLLHNIAGKAVGFGEALQPEFEE
                     YAAQTVKNAEALGESLQEHGFELVSGGTDTHLVLVDLRESHPDVTGKEAEEALEDVGIV
                     LNANTVPGETRSPFVT"
     gene            1512..3632
                     /locus_tag="EPL01_RS06345"
     CDS             1512..3632
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015301011.1"
                     /locus_tag="EPL01_RS06345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_135824410.1"
                     /transl_table=11
                     /translation="MIEWEETDSGLRVFDRTKTEVSIETDNWEATDAGYDIDRPLDETV
                     SGYVSELRLPPALVKANSLTSDEEYKHGGDAEAIDLDADEYLLNVSLNIKTYIRFSGAA
                     VVRKTEDYTELHLSFPEPTLVTFGFRSHHEAPVDTMTVPPTPEGIATAVTYSSSSHKTT
                     GADRSYPTLRGHPPKIELGGQTEIPDPVREERFDTGIEMVVPDDFRYAFVAAPLAYYLQ
                     AEMTVEDRRAPVLRAPEAGVEYPFTPLPGFQHEVVELLRRVFFLDCLVRNAGPYSWDLA
                     EMPLLDRVEIDAEATYEATPGEQLATYLDAPYERIDGELPEWHLAMHVEPVPDNATSLP
                     YFLDNLGLVYLPESTDLEKDELLNKSIDDFYRAGETPPRSPKLLPQKSAVGMARKGPGE
                     VKSVDRRDPILHEGKVNGWLADGVPIDVFKVVPEAYENRFKYLDPEGGDIDVTVILNDE
                     EMVDEHETAAEIYENRARDLPIDVTVLEHCSKDELASVFESSHDFVHYIGHCEEDGLRC
                     RNGNLSVESIDESKVQTFFLNACGSYYEGRDLVEKGSVAGAVTFTKVLNKQAAKVGVAF
                     AQLLINGYDIAHAIQLARRRIMMGKDYAVVGDGTHQLTQADNRIPALGEITEVDKDTEK
                     KYEIEYNSLTIDQIGGCYQLYLPDTDKLKLHGNDTTVAISQSELRSFLDRAKMPFIFDG
                     DLYWSEELGEKI"
     gene            4095..4919
                     /gene="tbsP"
                     /locus_tag="EPL01_RS06350"
     CDS             4095..4919
                     /codon_start=1
                     /gene="tbsP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977749.1"
                     /locus_tag="EPL01_RS06350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transcriptional regulator TbsP"
                     /protein_id="WP_135824411.1"
                     /transl_table=11
                     /translation="METESNLLDQEIAEILRTVLDEAPETMLVVNPSKDAIEQLINVAT
                     EYEGDLPELRMLAQSGTLKDVMEDFIVASNAADLIDAGSLSLRTTDDVPSNSLLVTEEV
                     VIALVTADDRVGGLTADDTEFVSEAYDSYSEQFESADDFSLRTPPLSRVRDTLGDEISP
                     DAQHDFDEVLSSMETARGDGDGLDEVTISLLVAAKNEALLYDISKWGEDVGIASKATFS
                     RTKTKLEDMGLIGTEKVPIDVGRPRLRLKLANDDLRNADTDQLASVAQSLLH"
     gene            5001..6758
                     /locus_tag="EPL01_RS06355"
     CDS             5001..6758
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006432692.1"
                     /locus_tag="EPL01_RS06355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_135824412.1"
                     /sec_met_domain="YcaO (E-value: 1.8e-65, bitscore: 216.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 8.7e-75, bitscore:
                     246.8, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAPTVGLVGTGPTIDAVAAALADVSAETVECGPRQVDTADFAVV
                     AGEVGAQVFAEANRTAREAGTPWLAVELGGLGGRALPEVDAAVSGFASATACWECLRTR
                     VAANPDDEGVANDSATDSPPEPDPATARFAGALAGREAATLLSGGESRVLGGVIEVPHA
                     ERTFLPVPGCEGCGHSESRSGLGREYRETDLEEALSRAEQAVDERVGVVSSIGEAESFP
                     APYYLANVTDTTRFSDAQAAEQAAGVSDDWNEALMKALGEALERYGAGVYRASDFETAS
                     AAELETPIAPSEFLRPEEWPDFSSEDEIAWVGGEELRTGESVHLPAEFVHFPPPEPRYK
                     PAITTGLGLGSSTVEALLAGLYETVERDATMIAWYSTFDPLGLSVESEQFDALRSRARA
                     KNLNVTALLVTQDVDVPVVAVAVHRDGEWPRFAVGSDADLNPDAAAESALAEALQNWME
                     LRSMGEEDAADADGAIGRYADFPEAARAFVSPETTVPSASVGPDRSLSGEAELDALLAR
                     LADENLPAYAARLTPRDVAQLGFEAVRVLVPDAQPLFTGDAFFGERAREVPSSLGFEFR
                     PDREHHPYP"
     gene            7103..8059
                     /locus_tag="EPL01_RS06360"
     CDS             7103..8059
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162322.1"
                     /locus_tag="EPL01_RS06360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_135824413.1"
                     /transl_table=11
                     /translation="MYPPTRLAGYAVLLVTPAALLARALVETGVSVPVAGLEVGLALAG
                     GVAVVAEHERALVAPDALDLGPSDAADALAVVGAAVVTYLLSVRAGLGPVVASALVGLL
                     AGVLADEIAVAAYCGSFVGMASPALFPSVSYLALAGLLSGVAFVAAERAFSGFGGKLGT
                     LALFGCATTAVLTDATYAGGSALSWTQAALVVPVSVAGAVGAVVLSVRLELGAVIGSAL
                     VGLIAGLGFPAIPIAPGETLAAAAFCASFVGMSSSERLDGEGRVALAGAVCGLVFVAVG
                     AAFAGAGGKLGTTAFVACVTTAGAERLFTAAVPLPNN"
     gene            8241..8414
                     /locus_tag="EPL01_RS06365"
     CDS             8241..8414
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977747.1"
                     /locus_tag="EPL01_RS06365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="WP_135824414.1"
                     /transl_table=11
                     /translation="MTSEFDLGCATCGTSLTRREVAAERLGFGSFDSLEVAECPNCGGR
                     YFPETTLERLGT"
     gene            8533..8907
                     /locus_tag="EPL01_RS06370"
     CDS             8533..8907
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017342685.1"
                     /locus_tag="EPL01_RS06370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cyclic nucleotide-binding/CBS domain-containing
                     protein"
                     /protein_id="WP_168215829.1"
                     /transl_table=11
                     /translation="MSSNDRPTVEDVMSTPLETISKDATVTEAAQQMREKDISALVVPT
                     TPRAIISSTDVLDAVADGRDTSELQVSDAMTTDVETATPDLYMEEVAAMMTTYGIKHLP
                     VVDDGYVGMVSSTDVTAHLS"
     gene            complement(8952..10079)
                     /locus_tag="EPL01_RS06375"
     CDS             complement(8952..10079)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007543631.1"
                     /locus_tag="EPL01_RS06375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF63 family protein"
                     /protein_id="WP_135824415.1"
                     /transl_table=11
                     /translation="MGTATESTDTERLWGGAITAILVALVGGALVFPERVYYGFIWHYF
                     WGPVLADAKSAQCAAYADGSVSYLYDAAACSAAAEPVAYPGYTLVSEVGYAITMLLALL
                     GVVFMLRRLDVGQDRELFYALFPFMLFGGALRVVEDAFDGLSPADAAITFPWNTLIISP
                     IIYFTMFAITLAALLGGIWLKRSEITDTYLYPLAGVGTALLATSIAYLTNLAFTTEYVD
                     FFPQISVVVLVGATLVTWLTWEAVKRYAPEVNQGTEKMGLVVIWAHSVDGVANVVGIDW
                     AKELGLPADLVPKHPVNRALIGVGEQFPEPVVDLIGTAWPFLVVKIVAAVFVVWVFDEE
                     IFEESPRYAILLLVAIVAVGLGPGTRDMLRATFGI"
     gene            complement(10146..10610)
                     /locus_tag="EPL01_RS06380"
     CDS             complement(10146..10610)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977744.1"
                     /locus_tag="EPL01_RS06380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_135824416.1"
                     /transl_table=11
                     /translation="MELDDTDREILEALQENARTPFSEIARRIDMSSATVHDRVNRMEE
                     AGVIEGYHAKVDPKALDYGISAVVGLQVEQGQEQDTLGRLEDIPGVQEVHLTTGSWDVL
                     MRVYAEDADKLRELMFENIAQMDGFARSQTMVILGTPYETEELPIEDGEI"
     gene            complement(10685..11479)
                     /locus_tag="EPL01_RS06385"
     CDS             complement(10685..11479)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1207: inositol monophosphatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009367436.1"
                     /locus_tag="EPL01_RS06385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="inositol monophosphatase"
                     /protein_id="WP_135824417.1"
                     /transl_table=11
                     /translation="MTKDAEERLAVAERAAKAGSEIAARNFRQGIEVETKSEKTDVVTE
                     ADREAQRRVIEVIREEYTEDAIVGEEEDELKEVPDEGPVWVIDPIDGTNNFVRSIPVWT
                     TSVAAVVDGEPVAAVNDAPALGETFLAGPEETRFNGESVTVSERTDPETFTVAPTFWWG
                     FDRRDEYAAMCRELVERFADIRRFGSAQITLGMVAAGSLEGAVTNVDPNPWDTVAGVHM
                     VRQAGGTVTDLHGDPWRHGSESLVASNGEAHDELVEATKRVE"
ORIGIN
        1 ccgtcgccca gcgacgagag gcatccggag accgaagcga cgaggccggc gctcgcggcg
       61 agcaaccggc gacgggaacc gttcatgcaa gacggatttt cctgtaagtg gaataagctt
      121 tctgcccgag aaagaagggg ataacggtct taacggcggc ttactcgtac agggggttcg
      181 cgtcgcagag ttcctgcacg tcctcggcga cttccgcctt cacgtcctcg tcaccgacgt
      241 tgttcaccac gcgggcgatg aggtcgccga cgtaccggca gtcctcctcg tcgaacccgc
      301 gggtcgtgag cgcgggcgtc ccggcccgaa tcccgctggt gacgaacggc gagcgcgtct
      361 cgcccggcac ggtgttggcg ttcagcacga tgcccacgtc ttcgagggcc tcctcggcct
      421 ccttgccggt cacgtccggg tgggactcgc gcaggtccac cagcacgagg tgggtgtcgg
      481 tgccgcccga gaccaactcg aagccgtgtt cttgcaggct ctcgccgagg gcctcggcgt
      541 tcttgacggt ctgggcggcg tactcctcga actcgggctg gagggcctcg ccgaacccga
      601 ccgccttccc ggcgatgttg tgcaggagcg gcccgccctg catccccgga atcacggccg
      661 agtcgatggc gtcggcgtac tcctcgtcgc agagaatcat gccgccgcgg cccgcgcgga
      721 tggtcttgtg ggtcgaaccg gtcacgaagt cggcgacgcc gaccggcgag gggtgttcgc
      781 ccgcggcgac cagtcccgtg atgtgggcca tgtcggcgag gtggtaggcg tccaccgcgt
      841 cggcggcctc ctgaatcgtc tcccactcga ctgcgcgcgg gtacgccgag tagcccgaga
      901 cgatgatgtc cgggtcgaac tcctcggcgt ggtcttggat agcctcgtag tcgagatagc
      961 ccgtctcggg gtcgacctcg tactgctcga cctcgtacaa ctcgcccgtg aagttggcgt
     1021 ggtggccgtg gctgaggtgg ccgccgtggg tcaggtcgag cgacagaatc ttgtcgccgg
     1081 gttcgagcat ggcgaagtag acgcccatgt tggcctgcgt gcccgagtgg ggctggacgt
     1141 tgacgtggtc ggcaccccac agctctttgg cgcgctcgat ggctaactcc tcgacggtgt
     1201 cgatgtgttc gcacccgccg tagtagcgcg acccggggta gccctcggcg tacttgttgg
     1261 tgaagacgct gccttgggcc tccatcacgg cctcgctgac gtggttctcg ctggcgatca
     1321 tcgccagcgt gtcgcgctgg cgctctacct ctgcttcgag ggcatctgcg acttccgggt
     1381 cggaatcgcg gacacggtcg tacttcatat caaaattcgg tgcgtccgcg agtataagct
     1441 tacctttcgc ggggaacgtc ccgggcgaca aaaatataaa gtcccggtga gacacgttgt
     1501 acaacgccga catgattgag tgggaggaga cggattctgg gttgcgcgta ttcgaccgca
     1561 cgaagacgga ggtgtcgata gagaccgaca actgggaggc gaccgacgcg ggctacgaca
     1621 tcgaccggcc gctggacgag acggtctcgg gctacgtctc ggagctacgg ctcccgcctg
     1681 cgctggtaaa agccaacagc ctcacctcgg acgaggagta caaacacggc ggcgacgccg
     1741 aggcaatcga cctcgacgcc gacgagtacc tcctcaacgt cagcctcaac atcaagacct
     1801 acatccggtt ctccggcgct gcggtcgtcc gcaagaccga ggactacacc gaactccacc
     1861 tctccttccc cgaaccgacg ctggtgacct tcggctttcg gagccaccac gaagcgcccg
     1921 tggacacgat gacggttccg ccgacgcccg agggcatcgc caccgcagtc acctactcgt
     1981 cgtcctcgca caagacaacc ggcgcggatc gctcgtaccc gacgcttcgg ggccacccgc
     2041 ccaaaatcga actcggcgga cagaccgaga ttcccgaccc cgtgcgcgag gagcgattcg
     2101 acacgggtat cgagatggtc gtccccgacg acttccggta cgccttcgtc gcggccccgc
     2161 tggcctacta cttgcaggcc gagatgaccg tcgaggaccg acgagcgccg gtcctgcgag
     2221 cgcccgaggc gggcgtcgag tacccgttca cgccgctccc gggcttccag cacgaggtcg
     2281 tcgaactcct ccggcgagtg ttcttcctcg actgcctcgt gcggaacgcg ggtccctaca
     2341 gttgggactt ggccgagatg ccactcctcg accgggtgga aatcgacgcc gaggccacct
     2401 acgaggccac gcccggcgag cagttggcga cgtatctcga cgccccctac gagcgcatcg
     2461 acggcgaact ccccgagtgg cacctcgcca tgcacgtcga gccggttccg gacaacgcca
     2521 cctccctgcc gtacttcctc gacaatctcg ggttagtcta tttgcccgaa tcgaccgact
     2581 tggagaaaga cgaactgctg aacaagtcca tcgacgactt ctaccgcgcg ggcgagactc
     2641 caccgcgttc gccgaaactc ctcccacaga agtccgccgt tgggatggcg cggaagggcc
     2701 ccggcgaggt caaatccgtg gaccgacgcg acccgatact ccacgagggg aaggtgaacg
     2761 ggtggctcgc cgacggcgtg ccgatagacg tgttcaaagt agtgcctgag gcctacgaaa
     2821 accggttcaa gtaccttgac ccagaaggcg gggacatcga cgtgacggtc atcctcaacg
     2881 acgaggagat ggtagacgaa cacgaaacgg cggccgaaat ctacgaaaac cgagcgaggg
     2941 acctcccaat cgacgtgacc gtcctcgaac actgctcgaa agacgaactt gccagcgtgt
     3001 tcgagtcgtc tcacgacttc gttcactaca tcggtcattg cgaggaggac ggcctgcgat
     3061 gccgaaacgg gaatctctct gtcgagagca tcgatgagag caaagtccag acgttcttct
     3121 tgaacgcctg tgggtcgtac tacgagggcc gagacctcgt ggagaaagga agcgtggcgg
     3181 gcgcggttac gttcacgaaa gtcctgaaca agcaggcggc gaaagttggt gtggcgttcg
     3241 cccagttgct gatcaacggg tacgacatcg ctcacgccat ccaactcgcc cgtcgtcgaa
     3301 tcatgatggg gaaagactac gccgtcgtcg gggatggaac tcaccaactc acgcaagcag
     3361 ataatcggat tccagcactc ggagagatta ctgaggtcga caaagacacc gagaaaaagt
     3421 acgaaatcga gtataattct ctaacgatag accaaattgg tggttgttac cagttgtact
     3481 taccagatac agacaagctc aaacttcacg gcaacgacac caccgtcgct atttctcaat
     3541 cagagcttcg ttcattcctt gaccgggcaa aaatgccgtt tattttcgat ggagacctat
     3601 actggtcaga ggaacttggc gaaaaaatat agtctgctct atggtcccat cgtactaccg
     3661 cctgcttcct gcgtactcaa ctcgaggaag actgaacttc caagcataag cgaaaacgcg
     3721 aagtccatcg ctcgcgggtt gtcagcgaac cattgtcgaa tcttgccttg catggcttgt
     3781 ttcgtgttaa tcagcaacac tactttattg tttcgtatct ttcatgcgcc cgtaggagaa
     3841 gaataaccca agaaatccga cgttaaacct acgcgagcgc gtcccgaatc tgggacgcgc
     3901 gagcgaaatc cccattacgt ctttcgattt tctccgctca ctgttaattg gcccggaatc
     3961 ttccatggaa cccaaacccc acaccgggac cggattagaa ttgtacgact gacttccaat
     4021 accatccgta taatccgtga tttccccccg ttactaacgt taaaattaag taggggtaaa
     4081 acgtttgtcg gtacatggag accgaatcga atttactcga ccaagaaata gccgaaatcc
     4141 ttcggacggt tctcgacgag gccccggaga cgatgctcgt cgtcaatccg tcgaaggacg
     4201 ccatcgaaca gctaatcaac gtcgccaccg agtacgaggg cgacctgccc gaactgcgca
     4261 tgctcgccca gtcgggcacg ctgaaagacg tgatggaaga cttcatcgtc gccagtaacg
     4321 ccgccgacct catcgacgcg gggtcgctgt cgctgcgcac gaccgacgac gtgccgagca
     4381 actccctgct cgtcaccgag gaggtcgtca tcgcgctcgt cacggccgac gaccgcgtgg
     4441 gcggcctgac cgccgacgac accgagttcg tctcggaggc ctacgactcg tactccgagc
     4501 agttcgagtc ggccgacgac ttctcgcttc gcacgccgcc gctctcgcgc gtccgcgaca
     4561 cgctcggcga cgagatcagc cccgacgccc agcacgactt cgacgaggtc ctctcctcga
     4621 tggagaccgc ccgaggagac ggcgacgggc tggacgaggt caccatcagc ctcctcgtcg
     4681 cggcgaagaa cgaggccctg ctgtacgaca tcagcaagtg gggcgaggac gtcggcatcg
     4741 ccagcaaggc gacgttctcg cggacgaaga ccaagctcga ggacatgggc ctcatcggca
     4801 ccgagaaggt ccccatcgac gtgggccgac cgcggctccg cctgaagctg gccaacgacg
     4861 acctgcgcaa cgccgacacc gaccagctcg ccagcgtcgc ccagagcctg cttcactgac
     4921 gaccagactc accatttttc gcgcggtagc tcccgagcga tagcaccgcg tttttccggt
     4981 ctccccgcct cgataccgcc atgaacgcac cgaccgtcgg actcgtcggg accgggccga
     5041 ctatcgatgc agtagcggcg gcgctcgcgg acgtgagcgc cgagaccgtc gagtgcggcc
     5101 cacgacaggt tgacaccgcc gacttcgccg tggtcgcggg cgaggtcggc gcgcaggtgt
     5161 tcgccgaggc gaaccggaca gcgcgcgagg ccgggacgcc gtggctcgcg gtcgaactcg
     5221 gtggtctcgg cgggcgcgcg ctccccgagg tagacgccgc agtctcggga ttcgcctcgg
     5281 cgaccgcctg ctgggagtgt ctgcggaccc gcgtcgcggc gaatccggac gacgaagggg
     5341 tggcgaacga ttcggcgacc gactcgcccc ccgaacccga cccggcgacc gcgcgcttcg
     5401 cgggcgcact cgccgggcga gaggccgcga ccctcctctc gggtggcgag tcgcgggttc
     5461 tgggcggcgt catcgaggtc ccccacgccg agcgcacttt cctcccggtg ccgggatgcg
     5521 agggctgtgg ccactctgaa tcccgctccg gcctcggccg cgagtaccgc gagaccgact
     5581 tggaggaggc tctctcgcgg gccgagcagg ccgtggacga gcgcgtcggc gtggtctcct
     5641 ctatcggcga ggccgagtcc ttccccgcgc cctactactt ggcgaacgtc accgacacga
     5701 cgcgcttcag cgacgcgcag gccgccgagc aggccgcggg cgtctccgac gactggaacg
     5761 aggcgctgat gaaggcgctg ggcgaggcgc tcgaacgcta cggcgcgggc gtgtatcggg
     5821 cgagcgactt cgagaccgcc agcgcggcag agttggagac cccaatcgct ccctcggagt
     5881 tcctccggcc agaggagtgg cccgacttct cgtccgagga cgaaatcgcg tgggtcggcg
     5941 gcgaggagct acggaccggc gagtcggtcc acctgcccgc cgagttcgtc cacttcccgc
     6001 cgcccgaacc ccggtacaag ccagccatca cgaccggcct cggcctcggg agttcgacgg
     6061 tcgaggccct attggcgggc ctctacgaga ccgtcgagcg cgacgcgacg atgatagcgt
     6121 ggtactcgac gttcgacccc ctcgggttgt cggtcgagag cgaacagttc gacgccctcc
     6181 ggtctcgcgc acgcgcaaag aatctaaatg taaccgccct gctggtcacg caggacgtgg
     6241 acgtgcccgt ggtcgcggtc gccgtgcatc gggacgggga gtggccccgg ttcgcggtcg
     6301 gctccgacgc cgacctgaac cccgacgccg cggccgagtc ggcgctggcc gaggcgctcc
     6361 agaactggat ggaactccgg tcgatgggcg aggaggacgc ggcggacgcc gacggcgcaa
     6421 tcgggcgcta cgccgacttc cccgaggcgg cccgcgcgtt cgtctcgccc gagacgaccg
     6481 tgccgagcgc gagcgtcggt ccggaccgct ccctctccgg ggaagccgaa ctcgacgctc
     6541 tcctcgcccg actcgccgac gagaatctcc cggcctacgc cgcgcgcctg acgccccgcg
     6601 acgtggccca actgggcttc gaggcggtcc gagtcttggt ccccgacgcc caacccctct
     6661 ttactgggga tgccttcttc ggcgagcgcg cccgcgaggt tccctcctcg ctcgggttcg
     6721 agtttcgccc cgaccgcgag catcatccct atccctgatt ttctatttta tctatccacc
     6781 gaccgatttc ggccggtgaa ccaaccgatt gatttcgacc ggcgaaccga tcatctgcag
     6841 tcctcggcgg actgaaaggg cgagcgcggt tcgcggaccg taggtccgtg gtcgtctcag
     6901 cgacccctat cggcgcgggc gttgctgaga gcgccgatat gtcgctgaga gaccgcgaac
     6961 cgcgcgaggg ctttcgagga cattgcggtc ccgtctgctc ctgcggttct gagcgcgagg
     7021 gctgttaaat cgaagtctca cccgtctccg aaccttaccg caaccacaac cgacaacacg
     7081 agtcgccgaa ctacccacgg gcgtgtaccc gccgactcgt ctcgctggct acgccgtact
     7141 gctggtgacg cccgccgctc tcctcgctcg cgcgctggtc gagaccggcg tctcggtccc
     7201 cgtcgccggt ctggaagtcg gactggccct cgccggagga gtcgcggtgg tcgccgaaca
     7261 cgagcgcgcg ctggtcgcgc cggatgcgct cgacctcgga ccgagcgacg ccgccgacgc
     7321 gctcgcggtc gtcggcgcgg cggtcgtgac ctacctcctg agcgttcgcg ccggactcgg
     7381 gccggtcgtg gcctcggcgc tcgtcggcct gctggcgggc gtcctcgccg acgaaatcgc
     7441 ggtcgcggcc tactgcgggt cgttcgtggg tatggcctcg cccgcgctgt tcccctcggt
     7501 gagctacctc gcgctggcgg gcctgctgtc gggcgtcgcg ttcgtcgcgg ccgagcgcgc
     7561 cttttcgggg ttcggcggca agttgggcac gctcgcgctg ttcggctgtg cgaccacggc
     7621 ggtcctgacc gacgcgacct acgctggagg gagcgcgctc tcgtggacgc aggccgcgct
     7681 cgtcgtgccg gtttcggtcg cgggcgcggt cggcgcggtg gtcctgagcg tccgcctcga
     7741 actgggtgcg gtcatcggct ctgcgctcgt gggtctcatc gctggcctcg gcttccccgc
     7801 gattccgatt gcccccggcg agacgctggc cgcggcggcg ttctgcgcgt cgttcgtcgg
     7861 catgtcctcc tcggagcgcc tcgacggcga gggtcgggtc gcgctcgcgg gcgcggtctg
     7921 cggcctcgtc ttcgtcgcgg tcggagcggc gttcgctggc gcgggcggga aactcggcac
     7981 gaccgcgttc gtcgcctgcg tcacgaccgc tggcgcggag cgcctgttca ccgccgcggt
     8041 tccgctcccg aacaactaat tgcttctttg ctcctctctt agatttctcc atcacctacc
     8101 tgtattttcg aactgagatg actccgaagt cagcggaccc cggtcggtga cttgcgtaag
     8161 caggggtttt atgtcgaaca ccgtcatacg cgctaacgta tgacattctc gaacagcgct
     8221 cgctatcggg gggtacgccg atgacgtccg agttcgacct cgggtgtgcg acctgcggga
     8281 cgtcgctgac tcgccgggaa gtcgcggccg agaggctcgg gttcggttcg ttcgactcgc
     8341 tggaggtcgc ggagtgcccg aactgcgggg gtcggtactt cccggagacg acgttggagc
     8401 gattgggaac ctgaaacgcc ggtccgggga gtggggcgtc gatttaatcg gatttccaga
     8461 aagtaaaatt ttgttctacg agagctgatt ccggggcaaa ccaccatacc gtccgcatcc
     8521 attgatagcg atatgagttc taacgacaga ccgaccgtcg aagacgtgat gtccacgccg
     8581 ctggaaacca tctcgaagga cgcgaccgtg acggaggcgg cccaacagat gcgagagaag
     8641 gacatcagcg cgttagtcgt tccgaccacg ccgcgagcga ttatcagcag tacggacgtt
     8701 ctcgacgccg tcgccgacgg gcgcgacacc tccgagctac aggtatccga cgcgatgacg
     8761 accgacgtcg agaccgccac gcccgacctc tacatggagg aggtcgccgc gatgatgacc
     8821 acctacggca tcaaacacct cccggtcgtc gacgacggct acgtcgggat ggtctcctcg
     8881 accgacgtga ccgcgcacct ctcgtaggcg acgcacgtcg ctcggagacg gaacagaaaa
     8941 atcgaatccc gtcagatacc gaacgtcgcc cggagcatgt ctcgcgtgcc gggaccgagg
     9001 ccgaccgcga cgatggccac cagcaggagg atggcgtagc gcggactctc ctcgaatatc
     9061 tcctcgtcga acacccagac gacgaacacc gcggccacga ttttcaccac gaggaagggc
     9121 cacgccgtcc cgatgaggtc cacgaccggt tcggggaact gctcgccgac gccgatgagc
     9181 gcgcggttca cgggatgctt gggcacgagg tctgcgggca gtcccaactc cttcgcccag
     9241 tcgatgccga cgacgttcgc cacgccgtcc accgagtgcg cccagatgac gaccagtccc
     9301 atcttctcgg tgccctgatt gacctcgggc gcgtagcgct tgaccgcctc ccacgtcagc
     9361 cacgtcacga gggtcgcgcc aaccagcacc acgaccgaaa tctgcgggaa gaaatcgacg
     9421 tactcggtcg tgaacgcgag gttggttaga taggcgatgg aggtcgccag cagggcggtg
     9481 ccgacgcccg ccagcggata caggtaggtg tcggtgattt ccgagcgctt gagccagatg
     9541 ccccctagca gcgccgccag cgtgatggcg aacatcgtga agtagatgat cgggctgatg
     9601 atgagcgtgt tccacgggaa ggtgatggcg gcgtcggcgg gcgagaggcc gtcgaaggcg
     9661 tcctcgacca cgcggagcgc gccgccgaac agcatgaacg ggaacagcgc gtagaacagc
     9721 tcgcggtcct gtcccacgtc gagtcgccgg agcatgaaga cgacgcccag cagcgctaac
     9781 agcatcgtga tggcgtaccc gacctcagag accagcgtgt agccgggata agcgaccggt
     9841 tcggccgcgg ccgaacacgc cgcggcgtcg tagaggtacg acaccgaccc gtcggcgtag
     9901 gccgcacact gagcgctctt ggcgtcggcc agcaccggcc cccagaagta gtgccaaata
     9961 aatccgtagt agacgcgctc cgggaagacg agcgcaccgc cgacgagcgc cacgagtatc
    10021 gccgtaatcg cgccgcccca gaggcgttcg gtatccgtac tctcggttgc ggtacccatg
    10081 tttgccaagg cggaaccgga cggttttacg gttccggtct cgcctcggtc gtacccgggt
    10141 ccgagttaga tttctccgtc ctcgatggga agttcctcgg tctcgtaggg cgtcccgaga
    10201 ataaccatcg tctgcgagcg ggcgaagcca tccatctgag cgatattctc gaacatgagc
    10261 tcgcggagct tgtcggcgtc ctccgcgtag actcgcatga gaacgtccca cgaaccggtc
    10321 gtgaggtgga cctcctgcac gccggggatg tcttcgagtc gcccgagggt gtcctgctct
    10381 tggccctgtt cgacttgcag gcccacaacc gccgaaattc cgtagtctaa ggccttgggg
    10441 tccaccttcg cgtggtagcc ctcgatgacg cccgcctcct ccatccggtt gacgcggtcg
    10501 tgaaccgtcg cactcgacat gtcgattcgg cgcgcgattt cgctaaacgg cgtcctcgcg
    10561 ttctcttgca gggcttctaa gatttcgcgg tcagtatcat cgagttccat atcctacatc
    10621 agattctttc agactatata tccttagaat tctcttcgtc agcggcgagt gattctgttt
    10681 cttctcactc gacacgtttc gtggcttcga ccagttcgtc gtgggcctcg ccgttcgaag
    10741 cgacgaggct ctccgaaccg tgtcgccacg ggtcgccgtg gaggtcggtg acggtgccgc
    10801 ccgcctgccg gaccatgtga acgcccgcga cggtgtccca cgggttcggg tccacgttcg
    10861 tcacggcccc ctccagcgac ccggccgcga ccatgccgag ggtgatctgc gcggacccga
    10921 accgccggat gtcggcgaag cgctcgacca gttccctgca catcgcggcg tactcgtcgc
    10981 ggcggtcgaa cccccaccag aacgtcggcg cgacggtgaa cgtctcgggg tcggtccgct
    11041 cgctgaccgt gacggactcg ccgttgaacc gggtctcctc ggggcctgcg aggaaggtct
    11101 caccgagcgc gggggcgtcg ttgaccgccg cgacgggttc gccgtccacc accgcggcca
    11161 cgctggtcgt ccacaccgga atcgaccgga cgaagttgtt cgtcccgtcg atggggtcga
    11221 tgacccacac cggcccctcg tcgggaacct ccttcaactc gtcttcctcc tcgccgacga
    11281 ttgcgtcctc ggtgtactcc tcgcggatga cctcgatgac ccgccgctgg gcctctcggt
    11341 cggcctcggt cacgacgtcg gtcttctcgg acttggtctc gacttcgatt ccctgccgga
    11401 agttccgcgc ggcgatttcg ctccccgcct tcgcggcgcg ctcggcgacc gcgaggcgct
    11461 cctcggcgtc cttggtcatg actcggtggt gggccgtcgc gggaaaagcg ttgtcggttt
    11521 gttggttctg actcagttcc atagggcttc gtatctggtc gacactgagg gtgctacttc
    11581 agtgcgtttc ctatcgccgt ctttatctaa ttttaggcgg gcaagatcct tccccaccga
    11641 gcaacgcaga ccgaaggccg agtagcgttg acgagatcga aactctcgtt cgcacattag
    11701 aacgcttcgc gttctatgaa ccagtagcga gggatacgcc cgccgtcatc aaccatca
//
