LOCUS       NZ_SNUX01000003        20219 bp    DNA     linear   CON 22-JUL-2024
DEFINITION  Shouchella lehensis strain DSM 19099 Scaffold3, whole genome shotgun
            sequence.
ACCESSION   NZ_SNUX01000003
VERSION     NZ_SNUX01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Shouchella lehensis
  ORGANISM  Shouchella lehensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Shouchella.
REFERENCE   1  (bases 1 to 932324)
  AUTHORS   Liu,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2019) Microbiology, Fujian Academy of Agricultural
            Sciences, Wusi Road, Fuzhou, Fujian 350003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-MAR-2019
            Assembly Method                   :: SMRT Link v. 5.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004375095.1-RS_2024_07_22
            Annotation Date                   :: 07/22/2024 02:06:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,132
            CDSs (total)                      :: 4,031
            Genes (coding)                    :: 4,012
            CDSs (with protein)               :: 4,012
            Genes (RNA)                       :: 101
            rRNAs                             :: 9, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:25:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 805068
            Orig. end                         :: 825287
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SNUX01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20219
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="glycocin"
                     /region_number="3"
                     /rules="(glycocin or sublancin)"
                     /tool="antismash"
     cand_cluster    1..20219
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(glycocin or sublancin)"
                     /kind="single"
                     /product="glycocin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20219
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10219](+)"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10219
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
     misc_feature    complement(912..922)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    939..957
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.27"
                     /tool="antismash"
     gene            complement(1556..2005)
                     /locus_tag="E2L03_RS14995"
                     /old_locus_tag="E2L03_14995"
     CDS             complement(1556..2005)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595424.1"
                     /locus_tag="E2L03_RS14995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_14995"
                     /product="VOC family protein"
                     /protein_id="WP_134259522.1"
                     /transl_table=11
                     /translation="MNAIAYLQFSGHAQEALDFYQHALQGTVKKVNFGSIPQDPSAPLS
                     GEEQDMIMESQITFSENILMISDVLPSMQSVIGDVTKGTNVIISLINGDVETNKRYFEE
                     LSKGGKILMPISSVPWSSSFGMLIDKFGIMWKFNSDASSFLNRLV"
     gene            2086..3000
                     /locus_tag="E2L03_RS15000"
                     /old_locus_tag="E2L03_15000"
     CDS             2086..3000
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017798648.1"
                     /locus_tag="E2L03_RS15000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15000"
                     /product="YafY family protein"
                     /protein_id="WP_134259524.1"
                     /transl_table=11
                     /translation="MQKVERLLSIIMILLKKEVVSASEFSQLFQVTKRTIQRDVEALSY
                     ANIPIYAKHGPSGGYALMEEYKFDKRLVNQKDIEHILVALDGFEQLTTNSDIQLTIQKI
                     KGMSPTNLASKMDVTFYQSIGRSETNEELSYWMKAIEHHWLLAFDYVDQQGRISHRLVE
                     PYKVQLREMHWYVVGFCLERNDFRTFKLTRITDMEKRGFFTPREQKMPQQQRTTQEPVK
                     PSMTSVKIELDIRVRDQFIERFGREAVVKKTERTYSAIIPLPESPYAFQFLAGFGNKIK
                     IIAPSHYRSHYTSFLKEALALYE"
     gene            complement(3205..3912)
                     /locus_tag="E2L03_RS15010"
                     /old_locus_tag="E2L03_15010"
     CDS             complement(3205..3912)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /locus_tag="E2L03_RS15010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15010"
                     /product="ABC transporter permease"
                     /protein_id="WP_038479601.1"
                     /transl_table=11
                     /translation="MYRLMKLELKKHKIGWYVKGTLIANASILAMLYFFTVIEKLENDI
                     IFQTSDEFFMFTGVLIRGTFIIFGAVLISKIVIDEFKHGTSLILFSYPISRKKLVTAKL
                     ILIFSITFLTMTLSTLFIVASFTGLNAFIGFSANLHVTQEQLSQELLLLIGFNVAAAGT
                     SLVPLYFGMRQFSTPATILSAVLIVIVTSSSLGPQFSLINIFYIPLALAVIGLIIAWVS
                     IKTINRLELNKPV"
     gene            complement(3927..4850)
                     /locus_tag="E2L03_RS15015"
                     /old_locus_tag="E2L03_15015"
     CDS             complement(3927..4850)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246292.1"
                     /locus_tag="E2L03_RS15015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15015"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_134259526.1"
                     /transl_table=11
                     /translation="MDYIVQTKGLTKRYDGHTVVSDVSFSIKKGEIYGFLGPNGAGKST
                     IMKMLINAIKPTSGEIELFGSPVREDSFRYLQRIGSMIEHPIFYEKLTARENLELHCAY
                     MGYHNKQTISDVLEWVDLSREAHKRPHQFSLGMRQRLCVARAILTKPELVILDEPINGL
                     DPEGIQSMRRLLKKLNKEYGMTLFISSHIIGEIEQIADTIGMIKDGTLIEETSMETLKE
                     QNTEYIELVTNDPKKAAVLLHDQLNITNFKIIENQRIRIFSSEMSHASISKALILEDIE
                     IHSIQTKQTTLEEYFIKKIGLRKNHQ"
     misc_feature    complement(4839..4859)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.02"
                     /tool="antismash"
     gene            complement(4945..5880)
                     /locus_tag="E2L03_RS15020"
                     /old_locus_tag="E2L03_15020"
     CDS             complement(4945..5880)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201546.1"
                     /locus_tag="E2L03_RS15020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15020"
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="WP_134259528.1"
                     /transl_table=11
                     /translation="MTVFLSIFSIVLLCGCLIQIQTKRTFQKQLRSIKKQLTDIVNGQA
                     SGPVLIGTDHKQMADLLVSINKLNDQNREHARQFIRTEQSMKRMLSNISHDLKTPLTVI
                     SGYAEMLNAKIDMPSQERQRILSHIHEKTDEMTMLIDSFFDLAKLEAGDKHVPLGTINL
                     TEICKQSILMFYELIETKGLDVQIDIPDSPIFALGNEEALHRVLANLVSNALRYGSDGN
                     VIGLHIYADETIVTIAVYDQGVGIRESDQQSIFERMYTLEESRNKDFQGSGLGLTITKK
                     LIEQMQGTISVTSIPHEKTSFICKLKRVHG"
     gene            complement(5877..6575)
                     /locus_tag="E2L03_RS15025"
                     /old_locus_tag="E2L03_15025"
     CDS             complement(5877..6575)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017695001.1"
                     /locus_tag="E2L03_RS15025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15025"
                     /product="response regulator transcription factor"
                     /protein_id="WP_134259599.1"
                     /transl_table=11
                     /translation="MNRLLLVEDDVAISEMVQTYFTNEGFTVVPAFTGQEAIQRFADDT
                     YDLVLLDLMLPELNGMDFLLTLRKTSYVPVIILSAKDSESDKAVGLGFGADDYITKPFS
                     MIELLARVKAAIRRTTQYKKATLPDVPAIVRIHEIELDLHSFIVKKKDQVIHLTSKEWK
                     LLKLFVENPKKVMTKEQIYYSVWEDHYYGDDHVINVHISRLREKIEDDPAKPMYIKTVW
                     GIGYKLGDVK"
     gene            6777..8102
                     /locus_tag="E2L03_RS15030"
                     /old_locus_tag="E2L03_15030"
     CDS             6777..8102
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /locus_tag="E2L03_RS15030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15030"
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="WP_134259530.1"
                     /transl_table=11
                     /translation="MKPQAFIKGAILIAGMFLVSCQNQSDERILYVAPDGDDRNPGTMD
                     QPFVSLQMAAEKATAGTTVFVREGTYVEPLLIQHSGTEQEPIRFEAYQGEDVFLSGKGM
                     EDDLDDVALIEIDGHSYITVDGFTIGDVTTNHVDKTVVGIFITGASQHIEIVNNTIRGV
                     STTAVGGNAHGIAVYGNEAITAIDINQNTLTDLKLGLSEALVVNGDVSQFTITNNHVYE
                     TDNIGIDVIGYEGIAIDQSKDYARQGVIQGNVVHHNSSYENPSYDKDYSAGGIYVDGGS
                     DVLIQGNTVYQNDIGIEATSEHKGKYANRIDILNNEVFDNRYTGIAIGGYDSERGGTRN
                     TVIAGNIVSHNDRVGLEGAQLLVQYYTFANRIEQNHFSASDTGLFISSEYDENDGMFFS
                     ENEYVVQEGVQARWLWGNKDAHSLKDFQRLTGSEHAAVFRSF"
     gene            complement(8423..8692)
                     /locus_tag="E2L03_RS15035"
                     /old_locus_tag="E2L03_15035"
     CDS             complement(8423..8692)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="E2L03_RS15035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="E2L03_15035"
                     /product="hypothetical protein"
                     /protein_id="WP_055735216.1"
                     /transl_table=11
                     /translation="MKTSKGHITIVFILFAIGGSVLTGIAGVGLLYLARWILHDQLFES
                     ISYVGAFFVAALPGFIGSLYWAYFFIKKEKRETKHLDDGHRHNE"
     gene            8730..8861
                     /locus_tag="E2L03_RS20865"
     CDS             8730..8861
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="E2L03_RS20865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_269083155.1"
                     /transl_table=11
                     /translation="MIVKLHLPSKHIEPNQEWNDDWMLSTHIVRYAFRRYPSITVMR"
     gene            complement(9125..9589)
                     /locus_tag="E2L03_RS15040"
                     /old_locus_tag="E2L03_15040"
     CDS             complement(9125..9589)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="E2L03_RS15040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15040"
                     /product="sensor histidine kinase KdpD"
                     /protein_id="WP_241742220.1"
                     /transl_table=11
                     /translation="MRLRSQQMPLQLKETRMEPFVRELVIDVLNEPAYKEASITFTGKA
                     PQLTRQVDQHLLKRGLLNFLYNALIHNKEGVSVHVTVTSTFIFIEDNGEGIQGDVHQIF
                     DRYYRSSNTTNINGTGLGMAIARDIMEAHGATVELTSQIGKGTSIKISFD"
     gene            10001..10219
                     /gene="gccF"
                     /locus_tag="E2L03_RS15045"
                     /old_locus_tag="E2L03_15045"
     CDS             10001..10219
                     /codon_start=1
                     /gene="gccF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     glycocin: glycocin"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033417.1"
                     /locus_tag="E2L03_RS15045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15045"
                     /product="glycocin F family RiPP peptide"
                     /protein_id="WP_038479632.1"
                     /sec_met_domain="glycocin (E-value: 6.6e-09, bitscore:
                     31.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKLKVALTDEEIDSYTSEGKGPFDVGGAAWCTYYIALCSTGRDS
                     GTCGWIFEQCGVGGGGGGGGCGTCVCR"
     gene            10344..11612
                     /locus_tag="E2L03_RS15050"
                     /old_locus_tag="E2L03_15050"
     CDS             10344..11612
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /locus_tag="E2L03_RS15050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15050"
                     /product="glycosyltransferase"
                     /protein_id="WP_166740326.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 4.7e-24,
                     bitscore: 81.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYDNDAKEKFFTNLKLYSSNITEKIESLRSHSIEPSQLKQFSLS
                     VIVICKDEERCIERCLNAINRNIGVNDEVIIIDTGSTDHTLNILNKYRAQSNFFIFEKE
                     WNNDFAEIRNFGIHKASKDWIVFIDADETVENDSFANLKSHLSIIDLLNVEAVCCPAIV
                     NTGGHVVQTVRRIIPNCQSIFYFGAVHEEPRPDDHSELFYLAFEDIVFHHDGYVEAVST
                     SKEKQKRNMALLYPMIDKEPLSPRWHYLLCRDGKGALNEDFYRDTLLKVITLCGDDELN
                     KEYKVRAVSDLIEFYLGAGDIDQAANNLEMLKELAPHMTDTLYWDILVQVIIFEYNYHQ
                     FIQRIIDYKKTHKELDYGSLNSNGFHLDHLLSRLFFNIREYGSSFAILKKLENAQYGEY
                     KAQYEELHKTLKNFLQAGEGNEK"
     gene            11602..13752
                     /locus_tag="E2L03_RS15055"
                     /old_locus_tag="E2L03_15055"
     CDS             11602..13752
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="E2L03_RS15055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15055"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_134259536.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.3e-26, bitscore:
                     86.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSKYFTKQLEENDCGPASVSMLLKYMWGTEITLAQLKILLFTNK
                     NGTTFLGMKKGLEKMGVESNVFKCVSSLEALNELDYPCITQISGGGQENHFVTLFKLTK
                     KHVYIGNPLKNQIEKVKIDTFLSMWIPFVLEIHKVTDNKKMMAYSSMESRKNNSIFKSL
                     YKIKKLIILSWVLSIIVYSLTVYLAGMFSTYFDVIIPNTAVGLIVSVTVIYLLAVVIQF
                     IIGYFNSILSIKSNNAVDKDLVENLVSNYFAQSYTFTEKFVSGELITRFTNISDIRTRY
                     LFFVQTLPLDALAIIITFVILFRINFYLSLLAFIPIIIFFLLLFLSKNRYENLSYDLFE
                     QQEYLNTELIESVDNIESIKNYNIAKRMKEKMIKRLDELYAIREKFMSFDQFQNLTKDS
                     IIKLFNICFFAFGSYLVINDNLASGMLLMFNSLVSNVFNPFVNLTTIQATLHQGRVATL
                     RYEDIVNSVVESSENDTKLENKIDTIRIRDLSYSFNPSSHVLHNISFEIKQGEKIALVG
                     SSGSGKSTLAKLLAGYYKPEKGEILVNHLNINEISQESLLKKILYIPQDIQIFNDTILN
                     NILLYRNIDFEDVQKVSKLVGFDEIVEMMPDGYGTVIGSKGVELSMGQQQMLNITRYIL
                     SPPDVLILDEITNGLDVVRRNRIKQILINSTITVIFITHDLDLATSCDKIYSLNEEGMN
                     VITSKSDENLAQTIMNSLEKGR"
     gene            13779..14186
                     /locus_tag="E2L03_RS15060"
                     /old_locus_tag="E2L03_15060"
     CDS             13779..14186
                     /GO_process="GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /locus_tag="E2L03_RS15060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15060"
                     /product="co-chaperone YbbN"
                     /protein_id="WP_148305169.1"
                     /transl_table=11
                     /translation="MAAILTMVFIGFVSVSNLVATEASLYKTVTSDLLKEKQENQDSFI
                     AYFYQKNCAPCQQVRPIINDYIEETQNEVLAVDINEDENKNLLIEGFDINSTPIVIFVD
                     SGKEQERFSSVFGEEEFKERASKVYSNDGVN"
     regulatory      14366..14480
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            14598..16373
                     /gene="thiC"
                     /locus_tag="E2L03_RS15065"
                     /old_locus_tag="E2L03_15065"
     CDS             14598..16373
                     /EC_number="4.1.99.17"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009335527.1"
                     /locus_tag="E2L03_RS15065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15065"
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="WP_276338674.1"
                     /transl_table=11
                     /translation="MTTSEVNEKRVSFMSSFEGSEKVYVQGSRPDLRVPMREVQLEPTI
                     GIDGKEESNDAIRLYDTSGPYTDKTYNVNLKMGLPRIREEWIKERGDTEHYMGRAVQPS
                     DNGFQNRDATDKMAPKSEHKTRKSKPGHFVTQLHYARNGIITPEMEFVAIREGVESEFV
                     RDEVAKGRAIIPANINHPESEPMIIGRNFHVKINANIGNSAVSSSIEAEVEKMTWATRW
                     GADTIMDLSTGKHIHTTREWIIRNSPVPVGTVPIYQALEKVNGIAQDLTWAVFRDTLIE
                     QAEQGVDYVTIHAGVLLRYIPLTITRTTGIVSRGGSIMAQWCLHHHKESFLYTHFDEIC
                     DILKAYDISLSLGDGLRPGSIADANDEAQFAELETLGELTKRAWKYDVQVMVEGPGHVP
                     MHLIKENMDKQLALCEEAPFYTLGPLTTDIGPGYDHITSAIGAALIGWYGTAMLCYVTP
                     KEHLGLPNREDVREGVITYKIAAHAADLAKGHKGAQKRDNALSKARFEFRWRDQFHLSL
                     DPERAVEYHDETLPAESAKSAHFCSMCGPKFCSMRISHDIRKHATERDVRTKAEIDEGL
                     KAKAEEFKQAGGSLY"
     gene            16426..16566
                     /locus_tag="E2L03_RS20535"
     CDS             16426..16566
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="E2L03_RS20535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158318523.1"
                     /transl_table=11
                     /translation="MSQVQQRLSELQNEKQLLQSECDHKFSGNTHFIRCHICGRVEVLY
                     Y"
     gene            complement(16615..17751)
                     /locus_tag="E2L03_RS15070"
                     /old_locus_tag="E2L03_15070"
     CDS             complement(16615..17751)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012117058.1"
                     /locus_tag="E2L03_RS15070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15070"
                     /product="exosporium glycoprotein BclB-related protein"
                     /protein_id="WP_367613692.1"
                     /transl_table=11
                     /translation="MSCNHCHRFHCSCSTVQKFNRPQVNGVTGPTGPTGPTGPGGGGSG
                     ITGPTGPTGATGATGIGLTGPTGPTGATGDPGLTGATGATGVTGATGIGLTGVTGPTGA
                     TGDPGLTGATGPTGATGIGLTGVTGPTGATGDPGLTGATGPTGATGIGLTGVTGPTGAT
                     GIGLTGPTGPTGATGIGLTGPTGATGATGIGLTGATGPTGATGAAGSTAVIPFASGIVP
                     SVISTIAGGLVGTVNLVGFGSSSTGVTLGAFTVADVGEFSFRAPRAGTIDSLSVSFTST
                     VGVTLPIGTGAITATVYKATGAILGSTPFLPTTATVTLTPAVTGLITIGDTFTGTVTGL
                     AEPVLAGDLLLLVLQLASPDITFITVLTGNTSAGLSIT"
     misc_feature    complement(17740..17758)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.16"
                     /tool="antismash"
     misc_feature    complement(17741..17757)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 23.65"
                     /tool="antismash"
     gene            18047..18262
                     /locus_tag="E2L03_RS15075"
                     /old_locus_tag="E2L03_15075"
     CDS             18047..18262
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008970.1"
                     /locus_tag="E2L03_RS15075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15075"
                     /product="hypothetical protein"
                     /protein_id="WP_243839534.1"
                     /transl_table=11
                     /translation="MAQDKERISQVIEVSISGKKIMGVGSAGLNEWGNLLGKYDSKERA
                     MEVLEDIHRTIANNSQFAITYTMPTD"
     gene            18295..18705
                     /locus_tag="E2L03_RS15080"
                     /old_locus_tag="E2L03_15080"
     CDS             18295..18705
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487570.1"
                     /locus_tag="E2L03_RS15080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_15080"
                     /product="DUF4181 domain-containing protein"
                     /protein_id="WP_134259538.1"
                     /transl_table=11
                     /translation="MNTESVFGMEFLIISLAVVVLLLWFFQIVMRKWFQVEKKPFFSYN
                     HVNRKHAKWDYGIRISFMVVLIVNLLLTLNMQNWLIEPWYLLIIFLILLESVRAIFEKK
                     YADNKNEYKLTISQLLFSVFLIGIFIFIMINF"
     misc_feature    complement(18713..18722)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.87"
                     /tool="antismash"
     misc_feature    18756..18765
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 15.96"
                     /tool="antismash"
     gene            18772..20121
                     /locus_tag="E2L03_RS15085"
                     /old_locus_tag="E2L03_15085"
     CDS             18772..20121
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="E2L03_RS15085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="E2L03_15085"
                     /product="hypothetical protein"
                     /protein_id="WP_134259539.1"
                     /transl_table=11
                     /translation="MIHLTSALQIEQQIIDIGDGGEYESITEAIGELITSGATRSNSSV
                     TLNLLKGFVLKEQIHIENLNLDWMTIVSEDSIVPIQASAISETLIESRRPAFLGRNQAT
                     LPTIGVLFEYDTQTARCDGITVAFGSKVRLLPGAGVNYADRGLGAYYHSEAFCYMEGLT
                     EGGGGIGAGDVTGVSFRYCSNRAFMASFGSKINCARSILDYCSGDNAVYAIWGSFCDIY
                     QSNVSHATNTAVHSRDGSKVNARETNVSHSNRGFHASTGSTINASTSSAKNCTIGYHAT
                     GASTINAQESDASGASWEGFFAEKGSIINADRSLANDCREGFSARFNSIINADTSSAQR
                     ARNHGFLANETSSIQARNCVANQAMINGFLATRSSTINARGSNARSCNGNGFRAEEGSS
                     LNANSGDATGSGNEGLDVFRGSFISAQNSIGSLRLPERLNQLTREGAIFR"
ORIGIN
        1 ggtggcttac tccaatttta gtacaaatct tgcaggttat gtggtagagg aattgacagg
       61 tatggctttt gctgattatg tggagcaatt gattttaacg cctttagaaa tggagcactc
      121 cacatttgat gtgcgctatg atcgtattcc tggtttaatg gacgagaagg cagttggata
      181 cacgaagaaa ggtacggaat gggtcgctaa accaactgct tatatcaacg ataatccggc
      241 tggtggtttg acgacaacga caaatgacct tgcttatttt atgctggcac acttaaatag
      301 agaacaatct ggctcgtacg tattgtttaa cgaaccacaa acattggata cgatgcacca
      361 gacattatac acgcctcatc ttgatatggc aggaaatgcc cacgggtttt gggagcgtca
      421 agcaggggat tatcgtgtct tagagcatgg tggcaataca gatgcgttta gcgcacttct
      481 ttccatcgta cctgatgaac agtttggcat tgccgttatg acaaatatgg atggtgagat
      541 ggcaggtgcg agaagagaac taacggatgt tttgattggg aagtcgacag aaacgccaca
      601 agcgacagat ggtatgaatc atagtgaagc agtagagggt cgctaccgtt ctgcacgctc
      661 tgttgaaaca gggccaatga aaatcatgcc ttttctctct gatggtgact ctgttatttc
      721 tgctaatcaa gagggtggga tcacgttatc cattgctgct ctcggtgtag aactagacta
      781 tgtagaaacg gctccatatt tgtttgaaaa agtgacagaa gagtcgtctt tacttgataa
      841 tgcaggcatg aatacaactc acttatactt taaaacgaac gatgctggtg aggttgagca
      901 attatcatat ggtgtcattg cagatgaatt accgatatcg tggttagcgt caccttatgt
      961 cgcttatgga ataacaggag gcgctgcact gttttcactt gttggtcttg tatggggaat
     1021 agttggcttt atacgaaaaa agaagcacca aaccctcatc acttctttgc ataaaggggc
     1081 aatagcgtgg tctttcgttg gcgttctgac tcttggtctt tttggcgcca ttgttgttca
     1141 attggcaagc aatccgtttc agccttggtc taatttcacc ttctacgtga tcggattttg
     1201 gctccttcta ttcgcatttc tcgcggtgct ttatctaatt gttaaagggt ggcacaagca
     1261 aaaaagcttc tacttaataa aggctttcat tgtgctacta ggctgtttat tggcaagttt
     1321 gttttacttg ttattctttc ttaactttct ctatctctaa aggatcaaga ggcttggaca
     1381 aaagtagata gacagaccaa aacgacgaac attcctgaaa ataggaacgt tcgtcgtttg
     1441 tttatatgaa aatgagcaga aggattctcc tgtagcggtg ctactcagca tattttagac
     1501 tattcgtctt ttcaactact acttttcgtt atgtcccagc ctctttttaa tgacgttaaa
     1561 cgagtcgatt caaaaagcta cttgcatcgc tattgaattt ccacataata ccgaacttgt
     1621 ctatcagcat gccgaaactg gatgaccaag gaacagaaga aatgggcatc agtatcttcc
     1681 caccttttga aagttcctca aaataacgtt tatttgtttc cacgtctcca ttaatcaagc
     1741 ttatgatcac gttcgttcct tttgtgacgt cgcctatgac gctttgcata gaaggaagta
     1801 cgtctgagat catcaggatg ttttcggaaa acgtaatctg agattccatg atcatatcct
     1861 gttcttcacc actaagtgga gcagaagggt cttgcgggat agaaccaaag ttcaccttct
     1921 taacggttcc ttgtaatgca tgttgataaa aatcgagtgc ctcttgtgcg tgcccactaa
     1981 attgtaaata tgcgattgcg ttcattttgt ctaacctcct cattggtcta ttcgtagtat
     2041 actagacagt aggtgacaac tatctgtcgt atataggagg gtgttatgca aaaagttgaa
     2101 agacttcttt ccatcattat gatactgcta aaaaaagagg tggtgtctgc atcagaattt
     2161 agtcaactct ttcaagtgac aaaacgtaca attcaaaggg atgtggaagc gttaagctat
     2221 gcaaacatac ccatttatgc aaagcatggc ccaagtggag ggtatgcgtt aatggaagaa
     2281 tataaatttg ataagcggtt ggtgaatcag aaagacattg aacatatcct tgtggcgtta
     2341 gatgggtttg aacagctgac aacaaacagc gatatccagt tgacaatcca gaaaataaag
     2401 gggatgagcc ctacaaatct agcttcaaag atggatgtaa cgttttatca atcgattggt
     2461 agaagcgaaa caaatgaaga gctgtcttat tggatgaagg cgattgagca ccattggtta
     2521 ctggcgtttg attatgtaga tcaacaaggg cgaattagcc ataggctcgt tgaaccatat
     2581 aaggttcaac tgagggagat gcactggtat gtcgtgggct tttgcctaga gcgtaacgat
     2641 tttcgaacat ttaagttgac gcgaataaca gatatggaaa aaaggggatt tttcacacca
     2701 agagaacaga aaatgccgca acaacaaagg acgacacaag aacctgttaa accttctatg
     2761 acgagtgtga agatcgagct ggatattcgt gttcgagatc aattcatcga acggtttggg
     2821 cgagaagctg tagtaaagaa gacagaaagg acgtacagtg caatcatccc acttcctgaa
     2881 agtccatatg catttcaatt tcttgcaggc tttggtaaca agataaagat tatagctcct
     2941 agccattatc gtagtcacta cacgtctttt ttaaaagaag cgttagcatt gtatgaatga
     3001 tgtcagttta tttgtaatta agaagccgtc cattgtcatg ccattggcca tatagcttgt
     3061 tttcttttgt gttcgttaaa aatttgcttt ttagttccag catttatcaa ccccatcttt
     3121 ttgtctgaat aagtgtgatc tagttatcat ctttcttccc tttacgttgt taaaagccat
     3181 ggggccattg cccatagctt tctgtcatac aggtttattc aattctaagc gattaatcgt
     3241 ttttatagat acccatgcga tgatcagtcc aataacggct agtgctaacg ggatgtaaaa
     3301 gatgttaatt aaagagaatt ggggccctaa agaagaactc gttacgatta cgattaaaac
     3361 cgctgaaaga atggtagcag gcgttgagaa ctgacgcatt ccgaagtaaa gaggaacgag
     3421 agacgtacca gctgctgcta cgttaaaacc aattaaaagg agtaattctt gtgaaagttg
     3481 ttcctgagta acgtgtaagt tggcagaaaa gccaatgaac gcgttaagac ctgtaaagct
     3541 agctacaata aacagcgttg atagcgtcat cgttagaaag gttatactaa agataagaat
     3601 cagtttggca gttactagtt tctttcgact aattggatag gaaaataaaa tcagactggt
     3661 tccatgttta aattcatcaa tgacgatctt tgagatgaga acggcaccaa agataataaa
     3721 tgtccctctt attaacacgc cggtaaacat aaaaaattca tcacttgttt ggaaaataat
     3781 atcattttca agtttttcaa taacagtgaa gaagtagagc atggcaagaa tacttgcatt
     3841 tgcaatgagc gttcctttta cataccaacc gattttatgt ttttttaatt caagcttcat
     3901 taaacgatac atcaacaaat cctcctttac tggtgatttt tacgtaaacc gattttttta
     3961 ataaagtatt cttctaatgt cgtttgcttt gtttggatag aatgaatctc gatgtcttct
     4021 agaattaacg cttttgaaat gctggcatga gacatctctg atgaaaaaat gcgaatcctt
     4081 tggttttcga tgattttaaa gtttgttatg tttagttgat catgtaagag aaccgctgct
     4141 ttttttggat cgtttgtaac gagttcaatg tactctgtat tttgttcttt cagcgtttcc
     4201 attgatgttt cttctattaa tgtcccgtct ttaatcatac caatcgtgtc cgcaatttgt
     4261 tcgatttcac caatgatatg actggaaata aaaagggtca tgccatattc tttgttcagt
     4321 ttttttaaca atcttctcat agattgtatc ccctcagggt caagtccatt aatgggttca
     4381 tcaaggatga ccaattccgg ctttgtgagg atggctcttg cgacacataa gcgctgtctc
     4441 atacctagtg aaaattgatg aggcctttta tgagcctccc tagaaaggtc gacccactct
     4501 agtacgtcag aaatcgtctg tttgttatgg tagcccatgt aagcgcaatg tagctctagg
     4561 ttttcccgag cagttaattt ctcataaaag attgggtgtt caatcatgct gccaattcgt
     4621 tgtaagtaac gaaatgaatc ttctcttact ggggaaccaa atagctcaat ctcgccactg
     4681 gttggtttaa tcgcgttgat aagcatcttc atgattgttg attttcctgc cccattagga
     4741 ccaagaaagc cgtaaatttc ccctttttta atcgagaacg acacatccga tacaacggta
     4801 tgtccatcgt atcgttttgt aagacctttc gtttgaacaa tatagtccat gttattcctc
     4861 ctttagttcc tatactctta ctgtaacgat tgactgtttc ttttctctat cgtaaacctc
     4921 acaaaaatct caagaaaaaa aagactagcc atgtacacgt tttaatttgc aaataaagga
     4981 ggttttctca tgagggatgg acgttaccga aatggttcct tgcatttgtt cgatcaactt
     5041 cttggtaatg gttaagccta atccacttcc ttgaaaatct ttatttctgg attcctccaa
     5101 tgtatacatt cgttcaaaaa tcgactgttg atcggattca cgaatgccaa ccccttgatc
     5161 atatacggca atcgtaacga tggtctcatc tgcgtatatg tgtaatccaa taacgttacc
     5221 atctgacccg tatcgaagtg catttgacac taaattagct aaaactcgat gtaacgcttc
     5281 ctcattgcct agagcaaaaa tgggtgaatc aggtatgtca atctgaacgt ctaatccttt
     5341 cgtttcgatt aattcgtaaa acataagaat cgactgtttg cagatttctg ttaaattaat
     5401 ggtacctaat ggtacatgct tgtctcctgc ttcaagtttc gctaaatcaa aaaaagaatc
     5461 gatcagcata gtcatttcat ctgttttttc gtgaatgtga gaaagaatac gctgacgctc
     5521 ttgactaggc atgtctattt ttgcattgag catttctgca taacctgaaa tcaccgttaa
     5581 aggggttttc aaatcatgag aaatattgga gagcatacgc ttcatcgatt gttctgttcg
     5641 aataaattgg cgagcatgtt cccggttttg atcattgagt ttattgatcg aaacgagtag
     5701 gtctgccatt tgcttatgat ctgttccgat tagtacagga ccacttgctt gaccatttac
     5761 aatatcggtt agctgctttt ttatggaacg cagctgtttt tgaaaggtac gcttagtttg
     5821 gatctggatg aggcatccac atagaagaac gatgctaaag atggatagaa agacagtcat
     5881 ttaacatcac ctaatttata gccaataccc caaaccgttt taatatacat tggtttagcg
     5941 gggtcatctt caattttttc acgcagtcgg cttatatgaa cgttaataac atgatcgtct
     6001 ccataatagt gatcctccca aacggaataa tagatttgtt cttttgtcat gactttttta
     6061 gggttctcaa cgaacagctt taagagtttc cattcttttg aggtgagatg gatcacttga
     6121 tcttttttct tgacgataaa cgaatgaaga tccaattcaa tttcgtgtat tcgtacaatt
     6181 gctggaacgt cgggtagggt agccttttta tactgggtcg ttcggcgaat ggctgctttc
     6241 actcttgcta gtagttcaat catggaaaaa ggcttcgtga tgtaatcatc cgcaccaaaa
     6301 cctagaccga ctgctttgtc ggactcgctg tcttttgcag atagaataat aacagggacg
     6361 taactcgttt tcctaagcgt aagtaggaaa tccatgccat ttaattcagg taacattaag
     6421 tcaagtaaga ctaaatcata tgtgtcatca gcaaatcgtt gaattgcttc ttgcccagta
     6481 aaagcaggca caactgtaaa gccttcgttt gtaaaatagg tttgaaccat ctcactaatg
     6541 gccacatcat cttctacaag tagtaatcta ttcattctgg caccgtcttt ctcattataa
     6601 aataaagctc ttctcattaa accacatctt agcaactatt atcgtatagc acgcgatctt
     6661 cacaatcaac ttcttagaat gagagacatt cataaaaaaa gaatcatgag ccttttcaca
     6721 gcgtggtata atgcaaagat cattatgtag atagtagcag agagggacga aatgaattga
     6781 agccacaagc ctttattaaa ggagctattc ttatagcagg gatgttttta gtaagttgtc
     6841 agaatcaatc tgatgagcgt attctttatg ttgcaccaga tggtgatgat cgaaaccctg
     6901 gtacaatgga tcagccattt gtgagcctac agatggcagc tgagaaagcg acagctggta
     6961 caacggtttt cgttcgagag gggacctatg tagaacctct tctcattcag catagcggaa
     7021 cggaacaaga gccaatacgt tttgaagcgt atcaaggaga agacgttttt ctaagtggta
     7081 agggaatgga agacgattta gacgatgtgg ctttaattga aatagatggt catagctata
     7141 ttacagttga tggatttaca atcggagacg tcacgacaaa tcatgttgac aaaacggtag
     7201 taggtatatt cattactggt gcgagtcagc atattgagat cgtgaacaat acaatccgag
     7261 gggtctcaac aaccgctgtt ggaggaaacg ctcatggcat tgctgtttac ggaaatgaag
     7321 cgattacagc tattgatatt aaccagaata cgctaacaga cctaaagtta gggttaagtg
     7381 aggcattagt tgtgaacggg gatgtcagtc aatttactat taccaataac catgtttatg
     7441 aaacagataa cattggaatt gacgttattg gctatgaagg catcgccatt gaccaatcga
     7501 aagattatgc gcgtcagggt gttattcaag ggaatgtggt tcatcataat tcgagctatg
     7561 agaatccaag ctatgacaag gattatagtg ctggcggaat ttatgttgat ggcggaagtg
     7621 acgtattgat ccaagggaac acggtttatc agaacgatat tggaatagaa gcgacgtctg
     7681 aacataaagg caagtatgct aatcgtattg acatcttgaa taacgaggta tttgacaatc
     7741 gctatactgg tattgctata ggcgggtatg actctgaacg tggggggact aggaacacag
     7801 ttattgccgg aaatatcgtg tcacataacg atagagtcgg tttagagggc gcgcaactat
     7861 tagtgcaata ttatacgttc gcaaatcgaa ttgaacaaaa tcattttagt gccagtgata
     7921 caggtctttt tattagtagc gaatacgatg aaaatgatgg gatgttcttt agtgaaaatg
     7981 aatatgtcgt acaagaagga gtgcaggcta gatggttgtg gggaaataag gatgcgcact
     8041 cattgaagga tttccaacgg ctgactggca gtgagcacgc tgcggttttt cgttcctttt
     8101 aacggtaaga cttggaaggg gaaggaataa ataaaaaggc tgggacataa cgaaaagtag
     8161 tagttgaaaa gacgaatagt ctaaaatatg ctgagtagca ccgctacagg agaatccttc
     8221 gctttccggg gacacggcct cagcctcctc cgtggaaaac cgccactcca gagtcttcag
     8281 acacgtgctg atcccccagg agtcttcgga ttctccttcc gctcattttc atataaaaca
     8341 aacgacgaac gttcctattt tcaggaatgt tcgtcatttt ggtctgtcta tctacttttg
     8401 tcccagcctc ttttacgtgt catcattcgt tatgacgatg cccatcatct aagtgtttgg
     8461 tttctcgttt ttcttttttt atgaaaaaat aagcccaata tagagatcca ataaagccgg
     8521 gtaaggctgc tacaaaaaag gctcctacat atgaaataga ttcgaatagt tgatcatgca
     8581 aaatccatcg agctaaatag agcagtccaa ctccagcaat accagttaga acggagccac
     8641 ctatagcaaa tagaatgaat acgatggtta tgtgtccttt gcttgttttc atgtacaatc
     8701 tcttcctttg cgtcaatatt ctaggatgta tgattgttaa actacattta cctagtaagc
     8761 acatagaacc gaatcaggaa tggaacgacg actggatgct gtctactcac attgttcgtt
     8821 atgcgttccg acggtatcca tccataaccg ttatgagata aaataaaact aaataattat
     8881 tggtaatcgt tcgtttattt agcgagtaca tacctgttga atagggttga tgtttatacg
     8941 ccgtttttca gggaattata aacctattcc attcaatagg agtgatcaat atgttggtta
     9001 ggagaatcct tgcttcggct ataacaacgg tggcaatagc aggtgtgaat cgtatcgtgc
     9061 gccgagtcat tttaaagaaa taaaaagatc cggccttgta attaactagg ccggatcttt
     9121 ttttctagtc aaacgaaatt ttgatgctcg ttccttttcc tatttgactt gttaattcaa
     9181 ccgttgctcc gtgtgcctcc ataatatctc gtgcgattgc catacccaaa cccgttccgt
     9241 taatgttggt tgtattacta ctccgataat accgatcaaa aatctgatgc acatcgcctt
     9301 gtatgccttc accgttatct tcaatgaaaa taaatgtaga agtgacagtg acatgaactg
     9361 aaaccccttc cttattgtga atcaaagcat tgtacagaaa gttaagtagc ccccgtttta
     9421 gaaggtgttg atcgacctga cgtgtgagtt gaggtgcttt tcctgtaaac gtaatggatg
     9481 cttctttata agctggttca tttaatacat caatgactag ctcacgaaca aacggctcca
     9541 ttcgtgtttc ttttagctgt aaaggcattt gttgactacg taaacgcatc gtcaagttaa
     9601 aatcttcaat taactgttga atgtaacttg attgacgttc aataacttcc gcgtagtcga
     9661 cgacgaagta acatcttatg acgaagtaaa gtgggaagac attcctgctg aggcacaaga
     9721 caaattgtta caggtgccat aaagtttgat tctcgaagaa aagaagcgta aagagtccag
     9781 ggtgcaaaga tgcaccctgt ttttgttata tgtatctaat taaataggga tgctactcat
     9841 cacctttaaa aaagaagaaa aatagcgttt cattcccatt atgacgggga agaagtttaa
     9901 cgaagtgctc acttccgctt ttattataat ttttagtaaa caatggtaaa ccaatatatg
     9961 agaagattca atcttatcat tattgaagga ggaagaatat atgtcaaaat taaaagttgc
    10021 tttaacagat gaagaaattg attcgtatac gagcgaagga aaaggtccat ttgatgtagg
    10081 tggtgcggct tggtgcacct actatattgc actttgttct actggaagag atagtggcac
    10141 gtgtggttgg atttttgaac aatgtggtgt tggcggaggc ggtggaggag gaggttgtgg
    10201 tacttgcgta tgtagataga gtcaccaaac ctgaatcatc ccattaaata tattggttta
    10261 ccattgttcc tgaaaattat aaagaagttg tatggttcta ctctcaaatg ataggtgcat
    10321 aaaaagagaa caggagatga atgatgaagt atgataatga cgcaaaagaa aagtttttta
    10381 ctaatttgaa gttatactcc tcgaacatta ctgaaaaaat tgagtcttta agatcgcatt
    10441 caatcgaacc ttcacaatta aaacaattta gtctatccgt aattgttata tgtaaagatg
    10501 aagagagatg tattgagaga tgtttaaacg caattaatcg taatattggg gttaacgatg
    10561 aagttataat tattgacact ggatcgacgg atcatacgct gaacatcttg aataagtatc
    10621 gagcgcagtc gaattttttt atctttgaaa aggaatggaa caatgatttc gctgaaataa
    10681 gaaacttcgg aatacataaa gcttcaaaag attggatcgt ctttattgac gctgatgaaa
    10741 cagttgaaaa tgactcattt gctaatttga agtcccatct ttctattata gatttattaa
    10801 atgtagaggc ggtttgttgt cctgccattg taaatactgg tggccatgtg gttcaaacgg
    10861 ttagaagaat cataccaaat tgtcagtcca ttttttattt cggcgctgtt catgaagagc
    10921 ctcgacctga tgatcattct gagttattct atttagcttt tgaagacatt gttttccatc
    10981 acgatgggta tgtggaggcc gtctctacta gtaaagaaaa acaaaaacgg aatatggctt
    11041 tactttatcc tatgattgat aaagaaccat tgagtcctag atggcattac ttgctttgta
    11101 gagacggaaa aggtgcttta aatgaagatt tttatagaga cacgctctta aaggtaataa
    11161 cattatgcgg cgatgacgaa ttaaacaaag aatataaagt aagagcagta agcgacctaa
    11221 ttgaatttta cttaggtgct ggtgatattg atcaggcagc taataactta gaaatgttaa
    11281 aagaattagc accccatatg actgacactt tatattggga tatcttagta caagtcatta
    11341 tttttgaata caattaccac cagtttatac agcgcattat agactataaa aagactcata
    11401 aagaactaga ttatggaagt ctaaattcaa atgggtttca tcttgatcat cttctgtcta
    11461 gactcttctt taatattaga gaatatggaa gtagttttgc gatcttaaaa aaacttgaaa
    11521 acgctcagta tggagagtat aaagcacagt atgaggaact acataaaact ttaaaaaatt
    11581 ttttacaagc tggagaggga aatgaaaagt aaatacttca caaaacaatt agaagaaaat
    11641 gattgtggcc ctgcctcagt gtctatgtta ttaaagtaca tgtggggaac agagattaca
    11701 ctggcacaat taaagatttt actattcaca aataaaaatg ggacgacttt tttaggtatg
    11761 aaaaaaggac ttgaaaaaat gggagtcgaa tcaaatgtct ttaaatgtgt atcttcttta
    11821 gaagcgctaa atgaattgga ttatccatgc attacacaga ttagtggggg aggacaagag
    11881 aatcactttg ttactttatt taagttaaca aaaaaacatg tttatatagg caacccatta
    11941 aaaaatcaaa tagagaaagt taagatagat acgttccttt ctatgtggat tccttttgtt
    12001 cttgaaattc ataaggtaac agataataag aagatgatgg catattcatc aatggaatca
    12061 aggaaaaaca atagtatttt taagagctta tataaaataa aaaagcttat tatcttgtca
    12121 tgggttttat ccatcattgt ctattcccta acagtctact tggcaggaat gttctcgaca
    12181 tattttgacg ttattatacc aaatacggct gttggactca ttgttagtgt tacagtcatc
    12241 tatcttttag ctgtagtcat tcaattcatt attggttatt tcaattctat tcttagtatt
    12301 aaatcaaaca atgctgtaga taaagatttg gtagagaatc tagtatcaaa ctattttgca
    12361 cagagctata cgtttactga aaagtttgta agcggagagt tgataacaag gtttacgaac
    12421 attagcgata taagaactcg atacttgttc tttgtgcaaa cgttgccttt agatgcttta
    12481 gcgatcatta tcacgtttgt catattattt aggattaact tttatttgtc cttgttggca
    12541 tttataccga ttatcatctt ctttttactg ttatttttgt cgaaaaatcg atatgaaaat
    12601 ttaagctatg atttatttga acaacaagaa taccttaata ctgaactgat agaatcagtg
    12661 gataatatcg agagtatcaa aaattataac attgcgaaac gcatgaaaga gaaaatgatt
    12721 aagaggttag acgaactgta tgcgataaga gaaaaattta tgtcttttga tcaatttcaa
    12781 aacttaacta aggattctat cataaagctt tttaatattt gtttttttgc atttggttct
    12841 tacctagtta tcaatgataa tttagcatca ggtatgttat taatgtttaa ctctttggta
    12901 tctaacgttt ttaatccgtt tgttaactta acgactatac aagctacttt acaccaagga
    12961 agagtagcca ctttaaggta tgaggatata gtcaattctg tggtagagag ctcagaaaat
    13021 gatacgaaac tagaaaacaa aattgataca ataaggatac gagatttatc ttattcgttt
    13081 aatccaagta gccatgtttt acataatatc agcttcgaaa tcaaacaagg tgaaaagatt
    13141 gcactagtag gaagcagtgg ttcaggaaaa tcgacgttag ctaaattgct tgcaggttat
    13201 tacaaacctg aaaaaggtga aattctagtt aatcatttga atattaatga gatcagccaa
    13261 gagtctcttc taaagaaaat tttatatatc cctcaggata ttcagatatt taatgacacc
    13321 atattgaaca acattttgct ctatagaaat atcgattttg aagatgttca aaaggtatca
    13381 aaattggtcg gttttgatga aatcgttgaa atgatgcccg atggttatgg tacagtgatt
    13441 ggctcaaagg gcgttgaatt atctatggga caacagcaaa tgttaaatat aacgcgatat
    13501 atcctctcgc cgcctgacgt tttaatatta gatgaaataa caaatggatt ggatgtagta
    13561 agaagaaata gaattaaaca aattttgata aactctacaa taacggtcat ttttataact
    13621 catgatttag acttggcaac ttcgtgcgat aaaatctatt cgctaaacga ggaaggaatg
    13681 aacgtaataa cgtctaaatc agacgaaaat ttggcacaaa cgattatgaa ttctcttgaa
    13741 aagggtaggt gattaaagtg aaaaaaataa cggtcatttt ggctgcaatc ttaacaatgg
    13801 tatttattgg tttcgtctct gtaagtaatt tagtagcaac tgaagcatcg ttatataaaa
    13861 cagtaacctc agatctacta aaagaaaagc aagagaatca agatagcttt atagcatatt
    13921 tctatcagaa gaactgcgca ccatgccaac aagtaagacc aattattaat gattatattg
    13981 aggaaacaca aaatgaagta ttagcagttg atataaatga ggatgaaaat aagaatctcc
    14041 taatagaggg ttttgatatt aatagtacgc caatagtcat ttttgttgat tcaggaaaag
    14101 agcaagaaag attttcgtct gtttttggag aggaagagtt taaagagaga gcaagtaaag
    14161 tttatagcaa tgacggggtt aactaaagac tatttcacaa ttaagtgatc gttgaacatg
    14221 aaagtattga ataaggaatg aaagccagta ggatgttagg ctaaaattct attggctatg
    14281 aaaaagtctc tttgctgtaa aaggtttacg gataattgat tgacatttcc atttattgca
    14341 actacaatga taactgaatt caataatgac taactagggg agtccaatga gttgggctga
    14401 gagggaagcg cgatgaagct tcttgactct ttggacctga tctggatcat gccagcgtag
    14461 ggaagttaac ggttacactc tttacgtacc gtcttctatt tatgtatgtg atgctaggtt
    14521 caaaatggaa cctagctttt tggcgtggac aaaggttcga tctttgaatt ccatatttgg
    14581 aaaaaggaga gagcgacgtg acgacatcag aagtaaatga gaaacgagta agctttatgt
    14641 ccagttttga gggaagcgaa aaagtgtatg tgcaaggctc acgaccagat ttacgtgtac
    14701 cgatgaggga ggttcaactt gagcctacca ttgggataga tggcaaagaa gagtctaatg
    14761 atgccatacg cctttatgat acgagtgggc cttataccga taagacgtat aacgtaaact
    14821 taaaaatggg cttacctcga atccgagaag aatggattaa ggaaagaggc gacacagagc
    14881 actatatggg gagagcggtt caaccaagtg acaatggatt tcagaacaga gatgctaccg
    14941 ataagatggc gccaaaaagt gaacataaga cacgaaagtc aaaaccaggt cattttgtaa
    15001 cacagcttca ctatgcgaga aatgggatca ttacgcctga aatggaattt gtggcaataa
    15061 gagaaggcgt ggaatcagaa tttgtcagag atgaggtggc gaaagggcgt gccatcatcc
    15121 ctgcaaacat taaccatcca gaatcagagc caatgattat cggtcggaat tttcatgtga
    15181 aaatcaatgc gaatatcggt aattcggcag tgtcgtcatc gattgaagcc gaagtcgaaa
    15241 aaatgacttg ggccactcgg tggggagctg acacgattat ggatctatcg actggaaagc
    15301 atattcatac cactcgagaa tggatcattc gaaactcccc tgttccagta ggcactgttc
    15361 caatttacca agcacttgaa aaagtaaacg gcattgctca agatttaaca tgggctgttt
    15421 ttcgagatac gctcattgag caagcagaac aaggtgttga ttatgtcacc attcatgcag
    15481 gtgtcttact gcgctatatc cctttaacca tcactcgaac aacaggtatt gtttcacgag
    15541 gtggttccat tatggcccag tggtgtttac accatcataa agagagtttt ttgtacaccc
    15601 attttgatga gatttgcgat attctaaaag cttatgacat aagtctctca ctcggtgacg
    15661 gtttacgtcc tggttccatt gccgatgcga atgacgaagc tcaatttgca gaacttgaaa
    15721 cgttagggga attgacaaaa cgtgcgtgga aatatgatgt gcaggtaatg gtggaaggac
    15781 ctggtcatgt gcctatgcat ttaataaagg aaaatatgga caagcagctc gccctttgtg
    15841 aagaagctcc tttttacaca ctaggtcctc taacaacgga tattggccct gggtatgatc
    15901 acattacgtc tgcaattgga gcagcgctaa ttggatggta cggaacggca atgctttgct
    15961 atgtaacgcc aaaagaacat cttgggctac ccaatcgtga ggatgtccga gaaggggtga
    16021 tcacctataa aattgcggct catgctgcag atttagcgaa aggtcataaa ggtgctcaga
    16081 aacgagataa tgcattatcg aaagcacgct ttgaatttag gtggcgtgat caatttcatc
    16141 tttccctcga tccggagcga gcggtggagt atcatgatga aacgttgcca gcagaaagtg
    16201 caaaatcagc tcatttttgc tcgatgtgtg gcccgaaatt ttgtagcatg agaatttctc
    16261 acgacattcg taaacacgct actgaaagag acgtacgaac gaaagcagaa atcgatgaag
    16321 gattaaaagc aaaagcagag gaatttaaac aggctggagg aagtctttat taataaagta
    16381 aaggatgagc tcgttaaaaa gagaatgtgc cgggtggaac gagagttgag tcaagtacaa
    16441 caacgattaa gtgaactaca gaatgaaaaa cagcttctgc aatcggaatg cgaccacaaa
    16501 ttttctggaa acactcattt tattcgctgt cacatatgcg ggagagtaga agtgctttat
    16561 tattaattaa ccaaaataga aagcgattcg catgagcgaa ccgctttttt atctttacgt
    16621 gattgataaa cctgcgcttg tgttaccagt taagaccgta ataaacgtaa tatctggaga
    16681 tgctaattgt aagacgagta atagtaagtc tccagcaagt acaggttcag ctaagccggt
    16741 tacggttcca gtgaacgtgt caccaattgt gattagacca gttacagcag gtgttaaagt
    16801 tactgtagca gttgttggca agaatggtgt tgatccaaga atagcacccg ttgctttgta
    16861 aacagttgcg gttatggcac ctgtaccaat aggtaaggtt acacctactg ttgaggtaaa
    16921 ggacacagat aatgaatcaa ttgtgccagc tcgcggtgct ctaaaagaga attctcctac
    16981 atctgcaact gtgaaagcac caagtgtgac gccagtagaa ctgcttccga agccaaccaa
    17041 gttgaccgtc cctactaagc caccagcaat tgttgaaata acagatggta caatccctga
    17101 tgcgaatggg atgacggcag tagaaccagc ggctccagta gcgccagtag gtccagtagc
    17161 gccagtaagt ccgattccag tagcaccagt agccccagtc ggcccagtaa gcccgatccc
    17221 ggtagcgcca gtaggtccag tcggtccagt aagcccaatt ccagtagcgc cagtcggccc
    17281 agtcacgcca gtaagcccaa ttccagtagc gccagtcggt ccagtagcgc cagtaagccc
    17341 aggatctcca gtagcgccag tcggcccagt cacgccagta agcccaattc cggtagcgcc
    17401 agtaggtcca gtagcgccag taagcccagg atctccagtg gcaccggtag gtccagtcac
    17461 accagtaagc ccaatcccag tagccccagt gaccccagta gctccagtag cgccagtaag
    17521 cccagggtct ccagtggcgc cggtcggccc agtcggtcca gtaagcccaa tcccggtagc
    17581 accagtggca ccagtcggtc cagtaggccc ggtaatacca gagccgccac cacctggtcc
    17641 agtaggtccg gttggtccag taggtccagt gacaccattt acctgaggtc tgttaaattt
    17701 ttgtacagtt gaacaagaac agtgaaaacg atggcagtga ttgcaagaca ttttcaatcc
    17761 acctccttct ttaagataac ctccacgcat aaatggcttt ttaaagtgta ttgaatagtg
    17821 agttaaaaat ggaggtgtac tatcctatga aacagaaaag tgttttgctt gtgcttttgt
    17881 tgtattttat cattcgaact caaagcccca ttaacgcttt aaactatagg gtcatccagt
    17941 tttatcagtt ttattgttga ataatgaaac atcgagaaca ttgaggacgt aaatacggat
    18001 agaagaaaat aataagagga aagagttgag cgtgatgttg tggataatgg cacaggacaa
    18061 agagagaatc agtcaggtta ttgaggtttc gatttctgga aagaaaatta tgggtgttgg
    18121 tagtgctggg ttaaatgagt ggggcaattt attaggaaaa tacgattcta aagaacgggc
    18181 gatggaagtg cttgaagaca ttcatcgtac aatcgctaat aatagtcaat tcgctataac
    18241 gtatacaatg ccaacagatt aagagtgttc tgaaatgaaa ggagggctag tttgatgaat
    18301 acagagtctg ttttcggaat ggagtttctt attattagct tagctgtagt ggtgctgtta
    18361 ctgtggttct ttcagattgt catgagaaag tggtttcaag tggaaaagaa accatttttc
    18421 tcatataacc atgtgaaccg aaagcatgca aaatgggatt atggtattcg gatctctttt
    18481 atggtcgtac taattgttaa tttgctactg actctcaaca tgcaaaactg gttaattgag
    18541 ccttggtatc tacttattat ttttttaatt ctgttagagt cggtacgggc tatttttgaa
    18601 aaaaagtatg cagataataa aaacgaatac aaattaacaa taagccaact tcttttttcc
    18661 gtttttctaa taggaatatt tatttttatt atgataaatt tttagaataa tcgttcgcct
    18721 tctctttcta gattatgaag tccatatatg aaaaggaagg ggaagaaaat aatgatccat
    18781 ttaacaagtg cgcttcagat tgaacagcag atcattgata ttggtgacgg aggggaatat
    18841 gagagcatta cagaagcgat tggagaactc attacttctg gtgcaactcg cagtaattca
    18901 agtgttactc ttaatctttt aaagggtttt gtgttaaaag agcagattca tatcgaaaat
    18961 ttaaatctcg attggatgac aattgttagt gaggattcaa ttgtgccaat tcaagcatca
    19021 gcgatttcag aaacattaat tgaatcaagg agacctgcct ttttgggacg aaaccaagct
    19081 acattgccaa cgataggggt tctgtttgaa tatgacacgc agactgctcg gtgtgatggc
    19141 attactgtag ctttcggatc gaaagtccga ttacttccag gagctggtgt gaattatgct
    19201 gatcgagggc tcggtgctta ttaccattca gaggcattct gctatatgga aggcttaaca
    19261 gagggtggcg ggggaatcgg agcaggcgat gtaacaggtg tatcctttcg atattgctcc
    19321 aatcgtgctt ttatggcgtc ttttggttcg aaaattaatt gtgctcgctc tatccttgat
    19381 tattgtagtg gcgacaatgc tgtttatgcg atatggggat ctttttgcga catctatcaa
    19441 tcaaatgtga gtcatgcaac gaatactgct gttcatagta gagatggctc gaaagtgaat
    19501 gcgcgtgaga caaatgtatc acattctaat agaggcttcc acgcttctac tggatcgacc
    19561 attaacgcta gtacatcatc tgcaaaaaat tgtacaattg gctatcatgc tacaggggca
    19621 agtaccatca atgctcaaga aagtgatgca agtggagcga gttgggaagg tttttttgcg
    19681 gaaaaaggaa gtataatcaa cgctgatcgc tcccttgcga atgattgtcg agaaggtttt
    19741 agtgcccgtt ttaattcaat aatcaatgca gacacttcta gtgcccagcg cgcgagaaat
    19801 catggattcc ttgctaacga aacgtctagc attcaagcaa gaaactgtgt agccaatcaa
    19861 gcgatgataa atggattcct tgcaacaaga tcttcaacca taaatgctcg tggttctaat
    19921 gcgcgatcat gtaacggaaa tggattcaga gcagaggaag gatccagtct aaatgcaaat
    19981 tcaggagatg ctacaggtag tggcaatgaa ggtttggatg tttttcgggg atcctttatt
    20041 tcggctcaaa attcgatcgg tagccttcga ttaccagaaa gattaaatca gcttacacga
    20101 gaaggggcca tctttcgtta acgtatacat tgagttaaag aaagaggctg ggacaaaagt
    20161 agatagacag accaaaatga cgaacattcc tgaaaatagg aacgttcgtc gtttgtttt
//
