LOCUS       NZ_SNUX01000002        10396 bp    DNA     linear   CON 22-JUL-2024
DEFINITION  Shouchella lehensis strain DSM 19099 Scaffold2, whole genome shotgun
            sequence.
ACCESSION   NZ_SNUX01000002
VERSION     NZ_SNUX01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Shouchella lehensis
  ORGANISM  Shouchella lehensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Shouchella.
REFERENCE   1  (bases 1 to 968217)
  AUTHORS   Liu,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2019) Microbiology, Fujian Academy of Agricultural
            Sciences, Wusi Road, Fuzhou, Fujian 350003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-MAR-2019
            Assembly Method                   :: SMRT Link v. 5.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004375095.1-RS_2024_07_22
            Annotation Date                   :: 07/22/2024 02:06:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,132
            CDSs (total)                      :: 4,031
            Genes (coding)                    :: 4,012
            CDSs (with protein)               :: 4,012
            Genes (RNA)                       :: 101
            rRNAs                             :: 9, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:25:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 292783
            Orig. end                         :: 303179
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SNUX01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5396
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(629..642)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.31"
                     /tool="antismash"
     gene            complement(863..2314)
                     /locus_tag="E2L03_RS07600"
                     /old_locus_tag="E2L03_07600"
     CDS             complement(863..2314)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /locus_tag="E2L03_RS07600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_07600"
                     /product="spore germination protein"
                     /protein_id="WP_166740220.1"
                     /transl_table=11
                     /translation="MRTLTAHLKQNVHLIQQRFHTKDAIRSLSVTIGLDIHAELLYLPN
                     TVDEQKIHSFLLAPIQQLVKQSVTYDQLGSIFKDGEPSVAYTVEECVDGLVNGRALLMF
                     NKEEGYLFNCSNWRVRSVEVPLSSSVYEGPASGLTEDISVNLNLLRNYYRSPNLVIETL
                     SIGSEAKKEISILSVHGVSNPAIVEEVKARLNNINVSQAIINQLATDALEGDGLLFPRT
                     MSIDRPDACAMALAAGRVVILVEGSPLALLAPSIFYHFFQNQDDYLSDFGKFGSRPLRY
                     SYFFIATFTPAIIVAIERFHLDLIPRDLHEQLIKTHDTLAPFTIELLFVILLMQVIMDG
                     SYRLPGNVIFAVTFIGTMLISDVATDVNLFHPVTIVIIGICYISSFPVLHRGLLSPIFF
                     MRLSFIILAHFFGFAGLFLGTTVLLILMAKLHSIGTPYLYPFLPFQPDKWKDTLFRGGL
                     QRVINHPNPLPFNRHQAKANRKQHI"
     misc_feature    complement(2401..2417)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.11"
                     /tool="antismash"
     gene            2459..2893
                     /locus_tag="E2L03_RS07605"
                     /old_locus_tag="E2L03_07605"
     CDS             2459..2893
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010198556.1"
                     /locus_tag="E2L03_RS07605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_07605"
                     /product="hypothetical protein"
                     /protein_id="WP_038476319.1"
                     /transl_table=11
                     /translation="MQDHRFISSFFPPGGGGGSIGPFPGQGGPGYPPGPPGSGSFPGFP
                     TPGGGSSQGPQPGQGGGEPPGPPPGNPPQLQSVSAFAVDPGAIRGCLFRYTYLRLNNGE
                     QFWFYPTFVGRHSVAGYRWFIFRWVYFGIDTRRISAFQCV"
     misc_feature    complement(2911..2922)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.25"
                     /tool="antismash"
     misc_feature    complement(3088..3102)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.52"
                     /tool="antismash"
     gene            3184..3678
                     /gene="ectA"
                     /locus_tag="E2L03_RS07610"
                     /old_locus_tag="E2L03_07610"
     CDS             3184..3678
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245195.1"
                     /locus_tag="E2L03_RS07610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_07610"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_035392952.1"
                     /transl_table=11
                     /translation="MAQTPVLTLEKPTVEDGASMWQLAETSTLDSNSVYKYIMMCEYFD
                     ETCIVARENDEVVGFITGFIPPKQADTLFIWQVGVAESQRGKGLALDLLERLIERDACK
                     NVRYVEATVTPSNKASQSLFKKLARSQGTECNVFDCFGEELFPDDQHEEEKTFRIGPIK
                     G"
     gene            3698..4981
                     /gene="ectB"
                     /locus_tag="E2L03_RS07615"
                     /old_locus_tag="E2L03_07615"
     CDS             3698..4981
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (26-362). E-value: 8.5e-82.
                     Score: 266.8. Matches aSDomain:
                     nrpspksdomains_E2L03_RS07615_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957573.1"
                     /locus_tag="E2L03_RS07615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_07615"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_038476324.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.5e-78, bitscore:
                     258.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTDMKIFEEKESEVRSYCRSFPAVFTKAKGSTMWDEEGNEYID
                     FFAGAGALNYGHNDEKMKQALVDYIMSDGITHSLDMATSPKASFLTAFNKIILEPRGLN
                     YKVMFPGPTGTNTVEAALKLARKVTGRTDIISFTNGFHGMTIGSLSVTGNAFKRKGAGI
                     PLTNVVTMPYDNFLTDELDTLDYLEQFLTEGGSGVDIPAAVILETVQGEGGINAARTEW
                     LQRLERLCKEHEILMIIDDVQAGIGRTGTFFSFEEAGLTPDIVCLSKSIGGYGLPFALT
                     LFKPELDIWEPGEHNGTFRGNNHAFITATVALDYWKTPDFQDSIQRKAELVHHFLTTIV
                     EKYPELKGTVKGRGLMKGIRCEDCSLAGSIAAEAFKRGLIMETAGFDDEVFKLFPAVTI
                     PEETLKAGLEIIEESVRAALGIKEPILN"
     aSDomain        3776..4783
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_E2L03_RS07615_Aminotran_3.1"
                     /evalue="8.50E-82"
                     /label="E2L03_RS07615_Aminotran_3.1"
                     /locus_tag="E2L03_RS07615"
                     /protein_end="362"
                     /protein_start="26"
                     /score="266.8"
                     /tool="antismash"
                     /translation="FTKAKGSTMWDEEGNEYIDFFAGAGALNYGHNDEKMKQALVDYIM
                     SDGITHSLDMATSPKASFLTAFNKIILEPRGLNYKVMFPGPTGTNTVEAALKLARKVTG
                     RTDIISFTNGFHGMTIGSLSVTGNAFKRKGAGIPLTNVVTMPYDNFLTDELDTLDYLEQ
                     FLTEGGSGVDIPAAVILETVQGEGGINAARTEWLQRLERLCKEHEILMIIDDVQAGIGR
                     TGTFFSFEEAGLTPDIVCLSKSIGGYGLPFALTLFKPELDIWEPGEHNGTFRGNNHAFI
                     TATVALDYWKTPDFQDSIQRKAELVHHFLTTIVEKYPELKGTVKGRGLMKGIRCE"
     gene            5001..5396
                     /locus_tag="E2L03_RS07620"
                     /old_locus_tag="E2L03_07620"
     CDS             5001..5396
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322942.1"
                     /locus_tag="E2L03_RS07620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_07620"
                     /product="ectoine synthase"
                     /protein_id="WP_038476327.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.9e-48, bitscore:
                     158.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVIKLEDVLNTERDVDGGNWRSQRLVVEKDGVGYSVHDTQIRAG
                     TETHLWYKHHLESVYIIEGEGEVETVKDGKVWPVKQFECYVLDEHDEHYLRAKTDMRMV
                     CVFNPPTTGEEIHDEDGAYQLPEHMKK"
     gene            complement(5434..6342)
                     /locus_tag="E2L03_RS07625"
                     /old_locus_tag="E2L03_07625"
     CDS             complement(5434..6342)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="E2L03_RS07625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="E2L03_07625"
                     /product="hypothetical protein"
                     /protein_id="WP_134258855.1"
                     /transl_table=11
                     /translation="MQRFLFLFSLCLCITGCTIPADTGGDTLTSVNDESFTGFVIDRTD
                     IGSLVSHEGGLTMFSNIPVAHGVGDRVRIHYRDVLDSFPSQADGSQATVVSSPKPDGAT
                     YSEKEAIQQALELARAFPSSDAMSAYPFVTYTHYVKEADVWEITIQIYGEEEHLIEIGS
                     NGVSVDHCEHSSLTDYSPACFTGYVIENRLISTENDLITFTNLIRSAEIGDRVSVEYTD
                     VKESYPASATAVSSKVVSPPKPDGATYSEKEAIQQALLYYNEQNRQPESDPVAFPVIHF
                     CTYHDETDTWTITINDVDIEI"
     misc_feature    complement(6469..6482)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.11"
                     /tool="antismash"
     gene            6561..7319
                     /locus_tag="E2L03_RS07630"
                     /old_locus_tag="E2L03_07630"
     CDS             6561..7319
                     /GO_function="GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /locus_tag="E2L03_RS07630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_07630"
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="WP_134258856.1"
                     /transl_table=11
                     /translation="MTAIYGHRGAMGVFPENTLPSFEEALSNGVDGIELDVQLTKDHQF
                     VVMHDPTVDRTTNGTGHVKDKTVKELKELSAGIPFTHLDNYCEADWKGITVPTLAEALE
                     LMAPYQIEVNIELKPNLSETEGIEKLLLDSVRPFQHALTLNYSSFHLPLIQRLRQLDKN
                     IELAWLVKHEIPYPADYLATNRINLYHLSKKLALKERSTFDEEGLVQQSRIWTVNEPEE
                     VKGLLKKKVKAIMTDFPEMALRIRKQQGNI"
     gene            7438..8172
                     /locus_tag="E2L03_RS07635"
                     /old_locus_tag="E2L03_07635"
     CDS             7438..8172
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="E2L03_RS07635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="E2L03_07635"
                     /product="hypothetical protein"
                     /protein_id="WP_134258857.1"
                     /transl_table=11
                     /translation="MDRVTSIEKIKIEKQAQRVKLIRTGKVTTLALGLACCANNVYTSR
                     LTSTGTTESKIHIQTKTKHHSNYLYSMYENDRLDQKIKVFGFKNTTDQVSDKWHFINEI
                     PELTNQLIAVNVVKERARIMMRRKYTSVQQKNGYKENTGESDQVGSKQTEDIQKTSAVE
                     EKDEKFNQEQPNQENRLNKYGKVVYVEQNRFAIETATGDKWFVVNDKKKINMLKPNIAV
                     SIYFHELDGKNCVTDLFITHKP"
     gene            complement(8305..9159)
                     /locus_tag="E2L03_RS07640"
                     /old_locus_tag="E2L03_07640"
     CDS             complement(8305..9159)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="E2L03_RS07640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="E2L03_07640"
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="WP_134258858.1"
                     /transl_table=11
                     /translation="MFTFSIPLAEPFNFNDAITSHGWWMLAPNVWDSKKARFYRTFPTR
                     NNDTIVGCIYMEASCLQIVTEVELTETDQDDILHYVSWMFRVNERFEPFYTLCQTHNEL
                     TDLPMKRKGRLLRSPTLFEDIVKVLLTTNTRWNQTIQMAEKLTYHLGEKVHGFGREFYS
                     FPTPQTILSAGEAFLMEHVRTGYRSHYILDAAHKAVNNPARYLQPSALNHFREIKGIGP
                     YAMNTLAMIVGRYDGLPVDSEYKKHVIGTYFNGIAPSKSELESVYDKWGDYKYLAYWFD
                     HYK"
     gene            complement(9162..9626)
                     /locus_tag="E2L03_RS07645"
                     /old_locus_tag="E2L03_07645"
     CDS             complement(9162..9626)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /locus_tag="E2L03_RS07645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="E2L03_07645"
                     /product="DinB family protein"
                     /protein_id="WP_055737110.1"
                     /transl_table=11
                     /translation="MSVKELILLQLGVIHNQKSWFVPLTHALENVSEHEAKWTPNDESN
                     SIWGIVNHLIFYNFRYLERFKNSHTTFEPVDSIAHTFERETTSWAETRNHIDQLLSEWR
                     EAVQKSELEDFSEAVTEYLTLLTLHNAYHIGQIVTIRKQQGTWPTELGVR"
ORIGIN
        1 agcatacaac actccactga cctttcattg attcgtctgt aacctcaacg aattcaccat
       61 ttctatatgc ttgtgctgag aatggttgta cttgtttacc gataagtgac atgaacaatt
      121 cctcctaatg ttaaatctaa gcatgtgtgc ttaaattgta ttaattctaa ttatgtacta
      181 attatcgaat aaagatcttc gtttgtcaat gcctttctcg aaagtttgac aaacgacaca
      241 gaatgtttct atttagcaat atgaaaggag tagttaactg aattttttat taataaaata
      301 aattccttct acgacagtgt agtaagcgct tactttttaa atgagaaaaa gcttatgtaa
      361 atgagagaaa gtaatagaaa gaaatcactg ttttttaaat ttaaaactgt gtttgttttc
      421 gttcgtgatg aagttgcact atcttttaaa atggttgacc gccttaggag aagagaaatc
      481 agaagaatgt tttttcaaat gtgtatcgct tttctcaata ggcatagata aggtgaaacg
      541 aggaatataa aaaaatgaga ctaggacaaa agtagataga cagaccaaaa tgacgaacat
      601 tcctgaaaat aggaacgttc gtcgtttgtt ttatttgaaa atgaacggaa ggagaacccg
      661 aagactcctg gggatcagca cgtgtctaga gactctggag tggtggtttt ccacggagga
      721 ggctgaggcc gtgtccccgg aaagcgaagg attctcctgt agtggttcta cgcagtatat
      781 tttagactat tcgtcttttc aaatacgact tttcgttatg tcctagcctc gtttttaatg
      841 gaggttgttt taaacttaca cattaaatat gttgctttcg atttgcttta gcttgatgac
      901 gattaaaagg aagtggattt ggatgattta tgactctttg taagcctcct cgaaaaaggg
      961 tatccttcca tttatcaggt tgaaagggaa gaaaaggata caaataaggt gtaccgatgg
     1021 aatgaagttt agccattagg atcaataaaa ccgtcgttcc taaaaacagt cctgcaaatc
     1081 caaaaaagtg agcaagaatg atgaacgata agcgcataaa gaaaatgggt gaaagcagcc
     1141 ctctatggag aactggaaaa cttgatatgt agcaaattcc gataatgacg atggtgaccg
     1201 gatgaaataa attaacatca gttgccacat cactaattaa catcgttccg ataaatgtaa
     1261 cggcaaagat aacattaccc ggcaatcgat aggaaccatc catgataact tgcatgagta
     1321 aaatgacgaa tagcaattca atcgtaaaag gagcaagagt atcatgggtc ttaattaatt
     1381 gttcatgtaa atctcttgga atgaggtcta aatgaaaccg ttcaatagcc acaataatag
     1441 ctggagtaaa cgtagcaata aaaaagtacg aataacgtaa tggtcttgac ccaaatttgc
     1501 caaagtctga taaatagtca tcttgatttt ggaaaaaatg ataaaaaata cttggtgcaa
     1561 gtaaagcaag aggggaacct tctactaaaa taacaactcg accggcagct agagccattg
     1621 cgcacgcatc aggccggtct atagacatcg ttcgcggaaa taataaacca tcgccttcta
     1681 acgcatctgt agcgagttga ttgataatgg cctgactaac attgatattg tttaatcgtg
     1741 ctttaacttc ttcaacgata gctgggtttg atacaccatg aactgatagg atggagattt
     1801 ctttttttgc ttcagaacca atagaaaggg tttctatgac aagattaggg cttcggtaat
     1861 aatttcttaa caaatttaga ttcactgaaa tgtcttctgt caaaccggaa gctggacctt
     1921 cgtaaacgct gctgctgagc ggaacttcta cacttctaac acgccaattt gaacagttaa
     1981 ataaatagcc ttcctcttta ttgaacataa gaagggctct cccgttaacg agaccatcta
     2041 cacattcttc aacggtataa gcaaccgatg gttctccatc tttaaagata cttcctaatt
     2101 gatcatatgt aaccgactgc ttaacgagct gttgaatcgg agctagtaaa aaagagtgaa
     2161 ttttttgctc atccactgta ttaggaagat agaggagctc agcatgaata tctaatccga
     2221 tggttacaga cagtgatcgg atcgcatcct tagtgtggaa acgttgttga ataaggtgca
     2281 cattttgttt taagtgagcc gttaatgtcc tcatgcgaat tcgccctttt tgactaagat
     2341 acatgtagtt tgtgtcaagt tagttgattt atacagttca ctgacataag gcaaaatcga
     2401 tgcaccattt ataggcaaat gcgtagaatg tatcaatgtc aatagggagg ttttaagtat
     2461 gcaagatcat cgcttcattt cgtctttctt cccacctggt ggtggaggtg gatcgattgg
     2521 cccctttcct gggcaaggtg gtccaggata tcccccggga cctccaggga gtggatcatt
     2581 cccggggttt ccaacgccag gaggtggttc ttctcaagga ccccaaccag gtcaaggtgg
     2641 aggagagccg ccaggtcccc cgccaggaaa tccgcctcag cttcaatcgg ttagcgcatt
     2701 tgctgtagat ccaggggcaa ttagaggatg tttgttccgg tacacgtact tgcgcttaaa
     2761 taatggcgaa caattttggt tttatccaac ctttgtagga agacattcag tcgctggtta
     2821 ccggtggttt attttcaggt gggtttactt tggaatcgat acacgacgaa ttagtgcttt
     2881 tcaatgtgtg taaaaaggaa agaaagaaga gaaatcattt tctcttcttt ttattttgaa
     2941 aataggaggg attgagacaa aggaggagga tatggaagaa aatctctctt agcttactct
     3001 taacgtcgat cactcctttt attgatacat aatctctact aatcctgttt gcgaaaatgt
     3061 gaccagcatc ctatcattaa aaaggattga aaatgttgat catttacaat ttgtggtttt
     3121 gattaaagag aagcgggtaa aagaaaggcg aacaccgctg tgaaaaaaat agaggaggcg
     3181 catatggcac agacaccagt attaaccctc gaaaaaccga ctgttgaaga tggagcttcg
     3241 atgtggcaat tagcggaaac atcaacgctt gattccaact cagtgtataa gtatatcatg
     3301 atgtgtgaat attttgacga aacgtgtatt gtagcccgtg aaaacgatga agtagttggc
     3361 tttattactg gttttattcc gcctaaacaa gcagacaccc tctttatttg gcaagttggt
     3421 gttgctgaat cacaacgagg caaagggctt gctttagatt tattagaacg actaatcgaa
     3481 cgagacgctt gtaaaaatgt aagatatgtt gaggcaactg taacgccttc aaacaaagca
     3541 tctcaatctc tttttaaaaa gctagcacgt agtcaaggaa ctgaatgtaa tgtgtttgat
     3601 tgttttggtg aggagctatt cccggatgat caacatgaag aagagaaaac gtttcgcatt
     3661 ggaccaatta agggttaaga atacaaggag gaaaaccgtg aataaaacag acatgaagat
     3721 ttttgaagaa aaagaatctg aagtaagaag ctattgcaga agtttcccag ctgtatttac
     3781 aaaagccaaa ggatcaacca tgtgggatga agagggaaat gagtatattg atttttttgc
     3841 aggcgctggt gcattaaact atggtcataa tgatgaaaaa atgaaacagg ccctcgttga
     3901 ctatatcatg agtgacggaa ttacccattc attagatatg gcaacatcac cgaaagcaag
     3961 ctttttaacg gcctttaata aaatcatatt agaacctaga gggttaaact ataaagtaat
     4021 gtttcctgga ccaacaggga caaatactgt ggaagcagct cttaagttgg ctcgtaaagt
     4081 aacaggacga acagatatta ttagcttcac aaatggattt cacggaatga caattggttc
     4141 actctcggtg actggtaatg cgtttaagcg taaaggtgct ggaataccat tgactaatgt
     4201 tgtaacgatg ccgtatgaca attttctaac ggatgaatta gatacacttg attatctcga
     4261 gcaatttttg actgaaggtg gatcaggagt tgacatacct gcagcagtaa ttcttgaaac
     4321 ggtacaaggc gaaggtggta ttaatgccgc acgtacggaa tggttacagc gattagaacg
     4381 attatgtaag gaacacgaga tattaatgat tattgatgat gtgcaagctg gtattggtcg
     4441 aacagggaca ttttttagct ttgaagaagc cggattgaca cctgatattg tatgtctgtc
     4501 caaatcaatt ggtgggtatg gtcttccttt tgctttaaca ttgtttaaac cggaattaga
     4561 tatttgggag ccgggtgagc ataacgggac ttttagagga aacaatcatg cctttatcac
     4621 agcaacagta gctttagatt attggaaaac accagacttt caagattcaa ttcagagaaa
     4681 agctgaactc gtgcatcact tcttaacaac cattgtggaa aagtatccgg aattaaaagg
     4741 tactgtaaaa ggtagaggtc ttatgaaagg aattcgttgt gaagactgtt cattggccgg
     4801 atcgattgct gcagaagcgt ttaaaagagg cttaatcatg gaaacagcag ggtttgacga
     4861 tgaagtgttc aaactgtttc cggcagtgac aattcctgaa gagacgttaa aagcaggatt
     4921 agagattatt gaagagagtg tgcgagcagc tttagggatt aaagaaccta ttttaaatta
     4981 acagctagga ggtcctacaa atgaaagtaa tcaaacttga agatgtttta aatacagagc
     5041 gagacgtaga cggtggtaac tggcgcagtc aacggctcgt cgtagagaaa gatggcgtgg
     5101 gctactctgt gcatgataca caaattcgtg ctggcacgga aacacactta tggtataagc
     5161 atcaccttga atcagtttat attatagaag gtgaaggcga agttgaaacc gttaaagatg
     5221 gtaaggtatg gccagtaaag caattcgaat gctatgtgtt agatgaacat gacgaacatt
     5281 acttacgagc aaagacagat atgagaatgg tttgtgtatt taatccacca actacaggcg
     5341 aagaaataca cgatgaagac ggcgcttatc aattaccaga gcatatgaag aagtaaatga
     5401 aacgaggaat tttcctcgtt tcttttttca tgtttatatt tcaatgtcca catcgtttat
     5461 cgttatggtc cacgtgtctg tttcatcgtg gtaagtacag aagtgaataa ctggaaaagc
     5521 aactgggtcc gattcaggtt gacggttctg ttcattataa tagaggagtg cttgctgaat
     5581 ggcttctttt tcagaatagg tggcaccatc cggctttggt ggagagacga ctttagatga
     5641 aacagcggta gcagaagctg ggtaactttc ttttacgtcc gtatattcaa cggatacgcg
     5701 atcgccaatt tcagctgaac gtataaggtt agtaaatgta attaaatcat tttcagtact
     5761 gataagccgg ttttcgatta cataaccagt aaagcaggca ggggaatagt ccgttaatga
     5821 agagtgctcg caatgatcca cggaaactcc attgctaccg atttcgatta aatgttcttc
     5881 ctctccataa atttgaatgg ttatttccca aacatctgct tcttttacat agtgtgtgta
     5941 tgtaacaaac ggataagcgc tcattgcatc acttgatgga aacgctcttg ctaattcaag
     6001 cgcttgctga atagcttctt tttcagaata ggtggcgcca tctggctttg gtgaagagac
     6061 gactgtcgct tgtgagccat ctgcttgaga gggaaaggag tctaaaacgt cgcgataatg
     6121 gattcgtacg cgatcaccaa ccccatgggc gacaggaatg ttcgaaaaca tcgtcaaccc
     6181 tccttcgtga gaaacgagtg aaccaatatc ggttctgtcg ataacaaatc cagtaaagga
     6241 ttcatcgtta acagaggtaa gcgtatcgcc accggtatcg gctggaatag tacaaccagt
     6301 tatgcacaag cataaactaa atagaaatag aaatcgttgc accttttttc ctcctttttc
     6361 acttatgtga acaaatggtt gcacacacaa cagaaatgaa tattctaaat actagtgtaa
     6421 agcataattc caatgcgtga aagcgatgtt gcagagtcat ttgtaaagtt ttacttaaaa
     6481 atgaacacat agtgtgaagg tgaaagtatc aaacggtata ataggagtga gaggtttttg
     6541 ttagaaagga atgattccct ttgacagcta tttacggaca tagaggggcg atgggtgttt
     6601 ttccagaaaa tacattgcca agttttgaag aagcgttatc aaatggtgta gacggtattg
     6661 aattagatgt tcagctgaca aaagatcatc aattcgtcgt catgcatgac ccgactgtag
     6721 acagaactac gaatggaact ggtcatgtaa aggataagac cgttaaggaa ttaaaagaac
     6781 tcagtgcagg tatacccttt actcatttag acaattattg tgaagcagat tggaaaggta
     6841 taaccgttcc aactcttgca gaggcgctag aattaatggc tccctatcaa atagaagtga
     6901 acattgagtt aaagccgaat ttgtctgaaa cagagggaat tgagaaatta ttgcttgata
     6961 gtgtgaggcc gttccaacat gcattaacac ttaattattc gtcgttccat ttgcctctta
     7021 ttcaacgtct ccgtcaattg gataagaata ttgaattggc gtggcttgta aagcatgaga
     7081 tcccttatcc agctgactat ttagcaacca atcgcattaa cttataccac ttgtctaaga
     7141 aactggcatt aaaagagcgt tctacttttg atgaggaagg gttagtgcaa cagtcacgta
     7201 tttggacagt aaatgagcct gaagaagtga aaggattgct taaaaaaaag gtgaaagcca
     7261 ttatgacaga ttttccagag atggcgttac gaatcagaaa gcagcagggc aatatctagc
     7321 tgttttcttt cttatgatta ggagttagac tttttgacta tgtctataag ctattgaaac
     7381 aggtttcata aaacgctatg cttaaaaagg gattgtaagg agggcttttt tacgaagatg
     7441 gatcgtgtaa ccagtataga aaaaataaaa atagagaaac aagctcaacg agtaaaactt
     7501 atccgaacag gtaaggttac cactcttgca ctaggtctag cttgttgtgc gaataatgtt
     7561 tacacttccc gccttactag tacaggcacc accgaaagta aaatacatat tcaaacaaaa
     7621 acgaaacatc attctaatta cttatattcc atgtatgaga atgaccggtt agatcagaaa
     7681 attaaagtat tcggttttaa gaataccact gatcaggttt cagataaatg gcactttata
     7741 aacgaaatac cagaattaac aaatcaattg attgctgtca atgtggtaaa agaaagagct
     7801 cgtataatga tgcgacgtaa gtacacttca gtacaacaaa agaacggcta taaggagaat
     7861 acgggtgaaa gcgatcaagt gggtagtaag caaacggaag acatacaaaa aacgtcagca
     7921 gttgaagaga aggacgagaa atttaatcaa gaacaaccga atcaagagaa ccgtctgaac
     7981 aagtatggaa aagttgtcta cgtagagcaa aataggtttg cgattgagac tgcaactggc
     8041 gataagtggt ttgttgttaa tgacaaaaag aaaataaata tgctgaagcc gaatattgct
     8101 gttagtatct actttcatga attagacgga aagaactgtg ttaccgactt gtttattaca
     8161 cataaaccat agaagaaatt gatttatact aaatccattc tctatcatac agttctatga
     8221 cgtttgttta gcatgtaacg tcgattcaga cgcaatcact gtaaataata gaaaaagatt
     8281 cgtgcatcag cacgaatctt tttcttattt atagtggtca aaccaatagg ctaaatactt
     8341 atagtcgccc catttgtcat agacgctctc aagctcgctt ttagaggggg cgattccgtt
     8401 aaaatacgtt ccaatcacgt gctttttgta ctcagaatca acaggcaaac cgtcataacg
     8461 accgacgatc atagcgaggg tgttcatggc ataagggccg attcctttta tctctcgaaa
     8521 gtgattgaga gcggatggtt gcaaatagcg ggcgggattg tttactgctt tatgagcagc
     8581 atccagaata tagtgacttc gataacctgt acgtacgtgc tccattaaaa aagcttctcc
     8641 tgctgaaaga atggtttgag gtgttggaaa agaataaaat tctcggccaa atccatgcac
     8701 tttctctcct aaatgataag taagtttttc tgccatttgg attgtttggt tccagcgagt
     8761 gtttgttgtt aaaagtactt tcacgatgtc ttcaaataga gtaggcgagc gtagcaatcg
     8821 accttttctt ttcataggta aatcggttag ctcattatgc gtttgacata atgtataaaa
     8881 aggctcaaac cgttcattca ctcggaacat ccatgaaaca taatggagta tgtcgtcttg
     8941 gtcggtttca gttaattcta cttctgttac aatttgtaaa caagaagcct ccatatagat
     9001 gcaaccaacg attgtatcat tgtttcttgt aggaaaggta cgataaaacc gtgctttctt
     9061 tgaatcccat acattcggag ctagcatcca ccatccgtgg gaagtaatcg catcattaaa
     9121 gttaaagggt tctgctaaag gaatggagaa tgtaaacacg attatcgaac tcctaattca
     9181 gtaggccaag ttccttgttg ttttcggatg gttacaattt ggccgatgtg ataggcattg
     9241 tgtagagtga gaagtgttaa atattcagta acagcttcgc taaaatcttc taattcggat
     9301 ttctgaacag cttcccgcca ttctgaaagc aattggtcaa tatggttcct tgtttcagcc
     9361 caggacgtgg tttctctttc aaatgtatga gcaatggagt caacaggttc aaatgtcgta
     9421 tgactgtttt tgaagcgttc taaataacgg aagttataaa aaatgagatg gtttacaatt
     9481 ccccaaatgc tgtttgattc atcattaggt gtccacttgg cttcatgttc agatacattt
     9541 tctaacgcat gggtcaacgg gacaaaccaa cttttttgat tatgaatgac acctaattgc
     9601 aagagaatta actcttttac actcatacaa ataacctcct cttgagtgga tgttaaagcg
     9661 tttccaaaag ttttttgcta ttatagcatg gatagaaaga tattgaacag gatgaaaggt
     9721 tcgattcgag cttgaaaaag atgtaaacga tgaagaagga ggaatgcaat ttggatttac
     9781 atttaaaagg aaaagtggtc attgtactcg ctgccagtaa aggtctagga agagggattg
     9841 cgcgccagtt tgcagaagaa ggagcgaaag tggtgttgtc gagtagacga cagaacgaac
     9901 tgattcaagt agtagatgat ataaaaaaag aaacaaataa tgctcatatc gtgcctcatg
     9961 tatgcgatat aacaaaagca gaagacattc aagagctagt caattttacc attaaaaagt
    10021 gggggagaat tgatgtatta attaacaatt caggtggacc tcctccagga ggcattgatc
    10081 aatttactga tgaagagtgg caacgtgcat acgagttgaa tctattaagc tatgttcgat
    10141 cgattcgtgc agtagtgccg caaatgcgca ttcaaggagg gggacatata gccaatgtag
    10201 cgtcctcttc gattaaacaa accctcgatg gattgttgct ctcgaatacg ttccgagcag
    10261 gggttgcagg actgtcaaaa agcttggcac aagagttagg gaaagatggc attcttatta
    10321 atactatcgg tcctgggcgt atagcaacag accgtttgct tgaactagat gaaacgaaag
    10381 ctgccaaagg aaatct
//
