LOCUS       NZ_SNXZ01000009        10819 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Labedaea rhizosphaerae strain DSM 45361 Ga0310470_109, whole genome
            shotgun sequence.
ACCESSION   NZ_SNXZ01000009
VERSION     NZ_SNXZ01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Labedaea rhizosphaerae
  ORGANISM  Labedaea rhizosphaerae
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Labedaea.
REFERENCE   1  (bases 1 to 207576)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 207576)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004362825.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 13:11:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,820
            CDSs (total)                      :: 7,753
            Genes (coding)                    :: 7,690
            CDSs (with protein)               :: 7,690
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 11 of 63
            Pseudo Genes (incomplete)         :: 55 of 63
            Pseudo Genes (internal stop)      :: 3 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:41:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 68817
            Orig. end                         :: 79636
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SNXZ01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10819
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10819
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10819
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5819](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5819)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            530..1039
                     /locus_tag="EV186_RS35270"
                     /old_locus_tag="EV186_10969"
     CDS             530..1039
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /locus_tag="EV186_RS35270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10969"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_208115944.1"
                     /transl_table=11
                     /translation="MHASFVEAMAEFLAECGGDPERTTMVGNELVDDRWRTPEGFADYV
                     ASVRADAVRPRQAGWMTQTTWWWCAGSTYLGRIDLRHGLTEALRTEGGHIGYDVRPSAR
                     CRGDATAMVAAVLPHARAMGIDAVLLTCNAGNLASRRVIEANGGELEAERGNRCRYWIT
                     LKSRCG"
     gene            1066..1596
                     /locus_tag="EV186_RS35275"
                     /old_locus_tag="EV186_10970"
     CDS             1066..1596
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /locus_tag="EV186_RS35275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10970"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_133853872.1"
                     /transl_table=11
                     /translation="MPRLRLPSLDSHASFVAAMAEFLAEGRGAPDDDTMVGAELRDGRW
                     RSAEGFAEFVAMLHEDEVRPPRAGWVTCTTWWWCEGSTYLGRIALRHELTEQLRTVGGH
                     IGYDVRPSARRQGHATAMLAAVLPHARERGIDELLLTCDPDNVGSQRVIEANGGVFEGE
                     REGKFRYWISVAG"
     gene            complement(1612..2802)
                     /gene="dinB"
                     /locus_tag="EV186_RS35280"
                     /old_locus_tag="EV186_10971"
     CDS             complement(1612..2802)
                     /EC_number="2.7.7.7"
                     /GO_function="GO:0003684 - damaged DNA binding [Evidence
                     IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="dinB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333441.1"
                     /locus_tag="EV186_RS35280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10971"
                     /product="DNA polymerase IV"
                     /protein_id="WP_133853873.1"
                     /transl_table=11
                     /translation="MYAEGPILHADLDSFYASVEQRDDPTLRSRPVIVGGGVVLAASYE
                     AKACGVRTAMGGRQALRLCPGAIVVPPRMKAYSEASKAVFKVFEQTTPLVEGLSIDEAF
                     LDVGGLAKIAGSPSTIAARLRAQVAREVGLPITVGVARTKFLAKVASRVAKPDGLLVVP
                     HDGELEFLHPLPVGHLWGVGKVTEEKLRERGITTVRQVAELGESYLVSVLGRGVGRHLF
                     ALAHNRDPRPVQVGRRRRSMGAQRALGSRPRQPAELDDILTGLIDKLARRLRGANRVCR
                     TVTLRLRFHDFTRATRSHTMPAPTAGTAMILPVARLLLAAALPMIAERGITLIGASLSN
                     LDNAEPLQLVLPFDDMSPPVLDTTLDAVRDKFGGASITRAGLLGQDPGIEMPMLPD"
     misc_feature    2801..2816
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.96"
                     /tool="antismash"
     misc_feature    complement(2802..2815)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.98"
                     /tool="antismash"
     gene            complement(2840..3037)
                     /locus_tag="EV186_RS35285"
                     /old_locus_tag="EV186_10972"
     CDS             complement(2840..3037)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EV186_RS35285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EV186_10972"
                     /product="hypothetical protein"
                     /protein_id="WP_133853874.1"
                     /transl_table=11
                     /translation="MNAPAGTTRHELPRARSADAADSAQPEPIDPKMAEAAELLASTER
                     ATAYATAAATLLAKAERAAR"
     misc_feature    complement(3030..3041)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     misc_feature    3089..3104
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.22"
                     /tool="antismash"
     gene            complement(3210..3854)
                     /locus_tag="EV186_RS35290"
                     /old_locus_tag="EV186_10973"
     CDS             complement(3210..3854)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334287.1"
                     /locus_tag="EV186_RS35290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10973"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_133853875.1"
                     /transl_table=11
                     /translation="MSGAVVVGAGPGIGRAVAARFAREGLPVAVIARRARTVRDTAATL
                     REFAVPVVELVADSTDEAGLRAALDKAAAELGPPDVLVYNAAVIQADGVGDLTARGHLD
                     AYAVNVVGAHTAAAHVAPWMAERGHGTIIVTGGMPAPVPGYVSLSIGKAAVRTLLRLVA
                     EQFGPSGVHVATVTVRGAVAPGTDYDPDDIAEHYWRLHAQPAGAWEFEVVH"
     gene            3884..5011
                     /locus_tag="EV186_RS35295"
                     /old_locus_tag="EV186_10974"
     CDS             3884..5011
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (4-364). E-value: 2.3e-82.
                     Score: 268.8. Matches aSDomain:
                     nrpspksdomains_EV186_RS35295_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456494.1"
                     /locus_tag="EV186_RS35295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10974"
                     /product="cysteine desulfurase family protein"
                     /protein_id="WP_133853876.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.6e-79, bitscore:
                     261.1, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 6.1e-07, bitscore:
                     22.5, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 6.4e-09,
                     bitscore: 29.1, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGPIYLDNNATTPVDARVAEAILPFLTGHFGNPSSTHAYGEPAR
                     TAVARAREQVAALLGCGDDEVVFTSGGSESDVLAIRGAALGRGDGRRQVITQATEHPAV
                     LAACDALRRLHGFEITVLPVDEHGLVDPTEAEAAISDRTALVTIMHANNETGTIQPIRR
                     LAALAHARGALFHTDAAQSIGKIVVDVADLDVDLLTVAGHKAYAPKGVGALYVRSGVTL
                     EPLVHGGGQERGLRGGTENVALIAGLGEAARLCAAALPRETERLRALRDRLWTRLSEAV
                     PAELNGHPIERLPNTLNVSLTGTVGADVLAGAPEVAAATGSACHEGVDEPSPVLAAMGL
                     PVERAKAALRLTVGRTTTAADVDAAAVALLRSATR"
     aSDomain        3896..4975
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EV186_RS35295_Aminotran_5.1"
                     /evalue="2.30E-82"
                     /label="EV186_RS35295_Aminotran_5.1"
                     /locus_tag="EV186_RS35295"
                     /protein_end="364"
                     /protein_start="4"
                     /score="268.8"
                     /tool="antismash"
                     /translation="IYLDNNATTPVDARVAEAILPFLTGHFGNPSSTHAYGEPARTAVA
                     RAREQVAALLGCGDDEVVFTSGGSESDVLAIRGAALGRGDGRRQVITQATEHPAVLAAC
                     DALRRLHGFEITVLPVDEHGLVDPTEAEAAISDRTALVTIMHANNETGTIQPIRRLAAL
                     AHARGALFHTDAAQSIGKIVVDVADLDVDLLTVAGHKAYAPKGVGALYVRSGVTLEPLV
                     HGGGQERGLRGGTENVALIAGLGEAARLCAAALPRETERLRALRDRLWTRLSEAVPAEL
                     NGHPIERLPNTLNVSLTGTVGADVLAGAPEVAAATGSACHEGVDEPSPVLAAMGLPVER
                     AKAALRLTVGRTTTAADVDA"
     gene            complement(5001..5819)
                     /locus_tag="EV186_RS35300"
                     /old_locus_tag="EV186_10975"
     CDS             complement(5001..5819)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228354.1"
                     /locus_tag="EV186_RS35300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10975"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_133853986.1"
                     /sec_met_domain="Linocin_M18 (E-value: 4.6e-80, bitscore:
                     262.0, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNHLMRELAPITGAAWEQIDDEARSRLAPNLAARRLVDVSGPHGW
                     DFSSVNLGRVRPLDQTTAGETGEGVRLRLRHVLALAEVRVPFTVSRVELENAERGADDL
                     DLDDLGRAARELALLENRAVFHGWAGADITGLVDSSTHRGLPLGADPTEYPQVAAKAVD
                     TLRNAGIGGPYALAIGPAGYERIVESTEHGGLLVLDHLRRILGGGKVVRSPGLTGALVV
                     SLDEGHFQLHLGQDISVGYSHCDADTAHLYLEESFTFRVVEPDAVVALTE"
     gene            complement(5899..6468)
                     /locus_tag="EV186_RS35305"
                     /old_locus_tag="EV186_10976"
     CDS             complement(5899..6468)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240685.1"
                     /locus_tag="EV186_RS35305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10976"
                     /product="hemerythrin domain-containing protein"
                     /protein_id="WP_133853877.1"
                     /transl_table=11
                     /translation="MPDITKLILDDHEWFRRQFAAMDDTTDPAELARIWAPLAALLDVH
                     AVAEEEIFYPALIRRGDDAEAETLDAIGDHNDIRDGVHEAALHPVGSTAWHAAVRKTRV
                     ANSEHMAEEEDDGLADFRRHADPGLRDELGERFLAFKRDHEGARDLDVSDKDPQEYVDE
                     VEAEAGVAPERGPDPSLGIGSLKEQQ"
     misc_feature    6510..6524
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.66"
                     /tool="antismash"
     gene            complement(6523..6786)
                     /locus_tag="EV186_RS35310"
                     /old_locus_tag="EV186_10977"
     CDS             complement(6523..6786)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680658.1"
                     /locus_tag="EV186_RS35310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10977"
                     /product="hypothetical protein"
                     /protein_id="WP_243754480.1"
                     /transl_table=11
                     /translation="MPDDRDNDDSTDPERDELDELGGLDDDETEVEVPEEIHTVCHLCG
                     GAGMVADPKFAVVGGVARTVDNGRQCPECESVGHFPGIVPPL"
     gene            6860..7234
                     /locus_tag="EV186_RS35315"
                     /old_locus_tag="EV186_10978"
     CDS             6860..7234
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470985.1"
                     /locus_tag="EV186_RS35315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10978"
                     /product="VOC family protein"
                     /protein_id="WP_133853878.1"
                     /transl_table=11
                     /translation="MACRISELVLDCRDPQRLAEFWCEVLGFVVLGTEDDGSIEIGPPE
                     GAGGLQPTLVLSPTADPKAGKLPLHIDVNATDRDQEAELRRLLALGARPADVGQTGEES
                     WHCLADPEGNEFCLLRTRLD"
     gene            complement(7292..7744)
                     /locus_tag="EV186_RS35320"
                     /old_locus_tag="EV186_10979"
     CDS             complement(7292..7744)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333205.1"
                     /locus_tag="EV186_RS35320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10979"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_133853879.1"
                     /transl_table=11
                     /translation="METAAFLLSQLGAHASSQFLARAAELDLTGPHVGVLRAIADDPGT
                     SQQALAQRLDILPSRVVAFVDDLEDRGLVRRERHPSDRRSHALRLTPTGEQVREQLRVL
                     GAEHNDRLLAPLSERERVTLTELLAKVAAAQGLRPGVHPGFRHLGR"
     gene            7794..9377
                     /gene="lnt"
                     /locus_tag="EV186_RS35325"
                     /old_locus_tag="EV186_10980"
     CDS             7794..9377
                     /EC_number="2.3.1.269"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016410 - N-acyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006497 - protein lipidation [Evidence
                     IEA]"
                     /GO_process="GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lnt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123: polyprenol-monophosphomannose synthase ppm1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680856.1"
                     /locus_tag="EV186_RS35325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10980"
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="WP_243754481.1"
                     /transl_table=11
                     /translation="MIAPTEADAEPADVPAPRRRDRLRPMLVPALLSVLGGVLAYLAEP
                     PRTVWWLAPIGLALLGWVLRGRRAKAGFGYGFLFGLGYLVPLLAWTGVFVGPVAWLPLS
                     AVCALLLALACAAISVVSKLPAWPLWAALLWVAGETFRAVFPFGGFPWPKLAFGQPEGI
                     YLPLAAVGGTPLIAFAVTLTGFGLAAFVRGLADGERTVGMLVARALAVMIPVAAAFASA
                     PLVGTDAQAGTVTVAAVQGNVPRAGLDFNAQRRAVLDNHVRRTEQLAADVKAGRVPKPD
                     IVIWPENSSDIDPLRNPDAYSEINQAAIDVGAPIAVGAVLVGDDGNPRNTVLLWEPGKG
                     PVDRYTKRQLQPFGETMPMRSFFRLFTSAVDRAGNFVAGDKPVVFTMGNARVALDTCYE
                     VAFDGVVRDSIAGSTILAVPTNNATFGRTEMTYQQLAMDRVRAVEHGRAVVVAATSGVS
                     ALVRPDGSVIEQTELFTPAALVERLPLRTATTLSDRLGATPEWVMDAAGLLALAAAVAL
                     GRRRSASPQE"
     gene            9438..10196
                     /locus_tag="EV186_RS35330"
                     /old_locus_tag="EV186_10981"
     CDS             9438..10196
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123: polyprenol-monophosphomannose synthase ppm1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673462.1"
                     /locus_tag="EV186_RS35330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_10981"
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="WP_133853880.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.7e-31,
                     bitscore: 103.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEQPSTAVEPVLVVIPTYNERENLGPITRRLRDALPKAHVLVVD
                     DGSPDGTGELADELAAKDELVHVMHRTSKAGLGAAYVAGFDWALERDYAVIVEMDADGS
                     HAPEDLPRMLAALEDADLVIGSRYVPGGHTVNWPASRKLISWGGNFYSRMALGAKVKDI
                     TAGFRAYRRQVLEKINLGNVASAGYCFQIDLAWRTIEAGFTVVEVPITFTERQVGDSKM
                     SGSIVREALLRVTKWGLRRRGTQLRNLVRR"
ORIGIN
        1 acgagtcgat cgcgacccgg cagtaccaga acggccgcac cgaggcgatg cgcgtggtga
       61 cgccggaggt cgtggccttc gtcgacgcga tgacctccga tgcgtccgcc gacgaacgca
      121 aggccgcttt ccttgccgcg gcggcgaagc acgtcgcgcg ggccaaggag tgccagatcg
      181 gtgccgcgcc ggagcagcac ctgtgggagc tgcagctgat cgcgcggcgc cggggttcgc
      241 aggagcccgc gctgtactcc tcgcccggct ggacgatcat gcgcgacgac tacctgagca
      301 cgagttcggc gccgtcgcag cacatccagt acttcgggtt cggctcgacc agcgcgaagt
      361 gcatcggggt ggcgtacgtg ctgctgccgg agcggttcca cctgtacctg gccacgcccc
      421 gctcggtggc cgaccagatg acggagttcg ccgagcagct gcgggtcgcg gtcgcccagt
      481 tgcgggagct gctgtcgtag cgccttcgtt gcgcctgccc tcggtcgacg tgcacgcgtc
      541 gttcgtggaa gcgatggcgg agttcctcgc cgagtgcggc ggcgaccccg agcgcaccac
      601 gatggtcggc aacgagctgg tcgacgaccg gtggcggaca cctgaggggt tcgccgacta
      661 cgtcgcgtcg gtgcgggccg atgcggtgcg gccacggcag gccgggtgga tgacgcaaac
      721 gacctggtgg tggtgcgcgg gctcgaccta cctcggccgc atcgacctgc ggcacgggct
      781 gaccgaagca ctgcggaccg agggcggcca catcggctac gacgtgcggc cctcggctcg
      841 ctgccggggt gacgcgaccg cgatggtggc ggcggtgctg ccgcatgcgc gcgcgatggg
      901 catcgatgcg gtgctgctca cctgcaacgc cggcaacctg gcctcgcgcc gggtgatcga
      961 ggccaacggc ggcgaactgg aggccgagcg cggcaaccgg tgccgctact ggatcacgct
     1021 gaaatccagg tgtgggtgag ccgggcgcct gctgtactcg gtcgcatgcc gcgcctgcgc
     1081 ctgccttccc tcgacagcca cgcctccttc gtcgccgcca tggcggagtt cctcgccgag
     1141 ggccgcggcg cgcccgacga cgacaccatg gtcggtgccg agctgcgcga cgggcggtgg
     1201 cgcagcgccg agggcttcgc ggagttcgtc gcgatgctgc acgaggacga ggtgcggcca
     1261 ccgcgggcgg gctgggtgac gtgcacgacc tggtggtggt gcgagggctc gacctacctc
     1321 ggccggatcg cgttgcggca cgagctgacc gagcagctgc gcaccgtcgg cgggcacatc
     1381 ggctacgacg tccggccgag cgcccgcagg caaggccacg cgacggcgat gctggcggcg
     1441 gtgctgccgc acgcacgcga gcggggcatc gacgaactgc tgttgacgtg cgacccggac
     1501 aacgtcggct cacagcgggt catcgaagcc aacggcggtg tgttcgaggg cgagcgcgaa
     1561 ggcaagttcc gctactggat cagcgtcgcc gggtgaagcc gggcctgctg cctaatccgg
     1621 gagcatgggc atctcgatgc ccgggtcctg gccgagcagc ccggcccggg tgatcgacgc
     1681 gccgccgaac ttgtcgcgga cggcgtccag ggtggtatcg agcacgggcg gcgacatgtc
     1741 gtcgaagggc agcacgagtt gcaggggttc ggcgttgtcc aggttcgaca acgacgcgcc
     1801 gatcagcgtg atgccccgct cggcgatcat cggcagtgcc gcggccagca gcaggcgtgc
     1861 caccggcagg atcatcgccg taccggccgt cggggcgggc atggtgtgcg agcgggtggc
     1921 ccgggtgaag tcgtggaagc gcagccgcag cgtcaccgtg cggcacacgc ggttggcgcc
     1981 gcgcaggcgg cgggccagct tgtcgatcag cccggtcagg atgtcgtcca gttcggccgg
     2041 ctggcggggt cggctgccca gcgcgcgctg cgctcccatc gaccggcgtc gcctgcctac
     2101 ctggaccggg cgcgggtcgc ggttgtgcgc gagcgcgaac aggtgccggc ccacaccgcg
     2161 gcccagcacc gagaccaggt aggactcgcc gagttcggcc acctgccgca cggtggtgat
     2221 cccgcgttcg cgcagtttct cctcggtgac cttgccgacg ccccacaggt gcccgaccgg
     2281 caacgggtgc aggaactcca gttcaccgtc gtgcggcacg accagcaggc cgtcgggttt
     2341 ggcgacccgg ctggccacct tcgcgaggaa cttggtgcgc gccacgccga cggtgatggg
     2401 caacccgacc tcgcgtgcga cctgcgcccg cagccgcgcc gcgatcgtcg acggcgatcc
     2461 ggcgatcttc gccaggccgc ccacgtcgag gaacgcctcg tcgatggaca agccttcgac
     2521 caacggcgtg gtctgctcga acaccttgaa gacggccttg ctcgcttcgc tgtacgcctt
     2581 catccgcggc ggcacgacga tcgcgcccgg gcacagccgc agcgcctgcc gcccgcccat
     2641 ggccgtgcgc acgccacagg ccttggcctc gtagctcgcc gcgaggacca caccgccgcc
     2701 gacgatcacc gggcgggagc gcagggtggg gtcgtcacgc tgctcgaccg acgcgtagaa
     2761 cgagtcgagg tcggcgtgca ggatcggacc ctccgcgtac acgaacacat gttcgcatac
     2821 cggggtgaca aaagagggct cagcgagcgg cgcgttcggc cttcgcgagc agggtcgcgg
     2881 ccgcggtcgc gtatgcggtg gcacgctcgg tcgaggcgag cagttcggcg gcctcggcca
     2941 tcttcggatc gatcggttcg ggctgagcgg agtcggcggc gtcggcggat ctggcccgcg
     3001 gcaactcatg ccgtgtcgtg cctgccggtg cgttcatgta ggtgacgcta ccaatgggca
     3061 cggacaacac ctatccgcgg cgtgtcggaa cgatcatgtt catcaggaag agtgagccgc
     3121 ctggcccgcc ggtccccggc aactggccta gtgcaccttg ccccgggagg cgccggcggg
     3181 cccgcgcatc cggtggctgc atcgatactt cagtgcacga cctcgaattc ccacgcgccg
     3241 gccggctggg cgtgcaggcg ccagtagtgc tcggcgatgt cgtcggggtc gtagtcggtg
     3301 ccgggcgcga ccgcgccgcg gacggtgacg gtcgcgacgt gcacgccgga cggcccgaac
     3361 tgctcagcca ccaagcgaag cagggtccgc acggcggcct tgccgatcga gaggctcacg
     3421 tagccgggca ccggcgcggg catcccgccg gtgacgatga tcgtgccgtg cccgcgctcg
     3481 gccatccacg gcgcgacgtg cgcggccgcg gtgtgcgcgc cgacgacgtt gaccgcgtag
     3541 gcgtccaggt ggcctcgggc ggtcaggtca ccgacaccgt cggcctggat gaccgccgcg
     3601 ttgtagacca gcacgtccgg cgggccgagt tcggcggcgg ccttgtcgag cgcggcccgc
     3661 agcccggcct cgtccgtgct gtccgcgacg agttccacca ccggcacggc gaactcccgc
     3721 agcgtggccg ccgtgtcccg cacggtccgc gcgcgtcgcg cgatcaccgc gaccggcaac
     3781 ccctcgcggg cgaagcgcgc ggccaccgcc cgcccgatcc ctggtcccgc cccgacgacc
     3841 acagccccgg acatccctgc tccgttcccg ctacgttcac cgcatgagcg gcccgatcta
     3901 cctcgacaac aacgcgacga cgccggtcga tgcccgggtg gccgaagcga tcttgccgtt
     3961 cctgaccgga cacttcggca acccgtcgag cacccacgcc tacggcgaac cggcccgcac
     4021 cgccgtggcc cgggctcgcg agcaggtcgc cgcgctgctc ggctgcggcg acgacgaggt
     4081 cgtgttcacc tcgggcggct cggaaagcga cgtcctcgcc atccgcggcg cggcgctcgg
     4141 ccggggcgat ggccgtcggc aggtgatcac gcaggcgacc gaacacccgg cggtgctcgc
     4201 cgcgtgcgac gccctgcgcc gcctgcacgg tttcgagatc accgtgctgc ccgtggacga
     4261 gcacggtctg gtcgacccga ccgaggcgga ggcggccatc tccgaccgca cggcgctggt
     4321 cacgatcatg cacgcgaaca acgagaccgg gaccatccag cccatccgcc gcctcgccgc
     4381 gctggcccac gcccgtggcg cgctgttcca caccgacgcc gcgcagagca tcggcaagat
     4441 cgtcgtggac gtcgctgacc tcgacgtcga cctgctgacc gtcgccgggc acaaggccta
     4501 cgcacccaaa ggggtcggcg cgctctacgt gcgctcggga gtcacgctcg aaccgctcgt
     4561 gcacggcggc gggcaggaac gcggcctgcg tggcggcacg gagaacgtgg cgctgatcgc
     4621 cggactcggc gaagcggcgc ggttgtgcgc ggcggccctg ccgagggaaa ccgagcggct
     4681 gcgcgccctg cgggaccggc tctggacacg gctgagcgag gccgttcccg cggagctcaa
     4741 cggtcacccc atcgagcgac tgcccaacac cctcaacgtc agcttgacgg gcacggtcgg
     4801 cgccgacgtc ctcgccggcg cccccgaggt cgccgcggcc accgggtcgg cgtgccacga
     4861 aggcgtggac gagccttcac ctgtgctggc cgcgatgggg ttgccggtcg agcgggcgaa
     4921 ggcggcgctg cggctgaccg tgggccggac caccacggcg gccgacgtgg acgcggccgc
     4981 cgtggcgttg ctcaggtccg ctactcggtg agcgcgacga cggcgtcggg ctcgacgacc
     5041 cggaaggtga agctctcctc gaggtagagg tgcgcggtgt cggcgtcgca gtggctgtag
     5101 cccacggaga tgtcctggcc caggtgcagc tggaagtgcc cctcgtcgag gctcaccacg
     5161 agcgcgccgg tcagcccggg ggagcgcacg accttgccgc cgccgaggat gcggcgcagg
     5221 tggtcgagca ccagcagccc gccgtgctcg gtgctctcca cgatccgctc gtacccggcg
     5281 ggcccgatgg ccagcgcgta cgggccaccg atgcccgcgt tgcgcagcgt gtcgacggcc
     5341 ttggccgcca cctgcgggta ctccgtcggg tccgcgccca gcggcaggcc ccggtgggtg
     5401 gagctgtcga cgaggccggt gatgtcggcc cccgcccacc cgtggaagac cgcgcggttc
     5461 tccagcagcg ccagctcacg ggcggcccgg ccgaggtcgt cgaggtcgag gtcgtccgcg
     5521 ccgcgctcgg cgttctccag ctcgacccgg gacaccgtga acggcacccg gacctcggcc
     5581 agcgccagca cgtgccgcaa ccgcagccgc acgccctcac cggtctcacc cgcggtggtc
     5641 tggtcgagcg ggcgcacccg gccgaggttc accgacgaga agtcccagcc gtgcggaccg
     5701 gagacgtcca cgagccggcg ggcagcgagg ttgggcgcga gccgggaccg ggcctcgtcg
     5761 tcgatctgct cccaggccgc cccggtgatc ggggcgagct cgcgcatcag gtggttcatg
     5821 ctcacgccca ccccctcgct gacgctccgg gccggcgtga gccggctcgc tgtgtccatg
     5881 gttcgctcgc aagctcgctc actgctgctc cttcaggctg ccgatgccca gtgacgggtc
     5941 cgggccacgc tcgggcgcca cgccggcctc tgcttcgact tcgtcgacgt actcttgcgg
     6001 atccttgtcc gacacgtcga gatcacgcgc cccttcgtgg tcccgcttga acgccaggaa
     6061 ccgctcgccc agctcgtcac gcaggccggg gtcggcgtgc ctgcggaagt ccgcgagccc
     6121 gtcgtcctcc tcctcggcca tgtgctcgct gttggcgacc ctggtcttgc gcacggcggc
     6181 gtgccacgcg gtgctgccca ccgggtgcag cgccgcctcg tgcacgccat cgcggatgtc
     6241 gttgtgatcg ccgatggcat cgagcgtctc ggcctcggcg tcatcaccgc ggcggatcag
     6301 cgccgggtag aagatctctt cctcggccac ggcgtggacg tcgagcaacg cggcgagcgg
     6361 cgcccagatc cgggccagct cggcgggatc ggtcgtgtcg tccatcgcgg cgaactggcg
     6421 ccggaaccac tcgtggtcgt cgaggatgag cttggtgatg tccggcatgg cacgaacgct
     6481 aaggcctcgc ccggcccggc gcagccgcgg ggccgaccgg tcctacagcg gcggcacgat
     6541 gcccgggaag tgcccgacgc tctcgcactc cgggcactgc ctgccgttgt cgacggtgcg
     6601 cgccaccccg cccaccacgg cgaacttggg gtcggcgacc atcccggcgc ccccgcacag
     6661 gtgacagacg gtgtggatct cctcgggcac ctcgacctcg gtctcgtcgt cgtcgaggcc
     6721 acccagctcg tcgagttcgt cgcgttccgg gtcggtgctg tcgtcgttgt cgcggtcgtc
     6781 gggcacgaac cgagcctaac gcccggcccg agacgaaacc cccgggcgca cgtcgcggcc
     6841 gggccgtagg gtcgtgatca tggcctgccg catttccgag ctggtgctcg actgccggga
     6901 cccgcagcgg ctcgcggagt tctggtgcga ggtgctgggg ttcgtcgtgc tcggcaccga
     6961 ggacgacggc tcgatcgaga tcgggccgcc cgaaggggcc ggcggcctgc agccgacgct
     7021 ggtcctgagc ccgacggccg accccaaggc aggcaagctg ccgctgcaca tcgacgtcaa
     7081 cgccaccgac cgcgaccagg aggccgaact gaggcggctg ctggcgctcg gggcgcgccc
     7141 ggccgacgtc ggccagaccg gcgaggagtc ctggcactgc ctcgccgacc cagaaggcaa
     7201 cgagttctgc ctgctgcgca cccgcctgga ctaggcgctg tcctgtggat ctcggcgcga
     7261 agaccgcgac cacggatccg caggacagtg cctagcggcc caggtggcgg aaccccgggt
     7321 gaacgcctgg gcgcagtccc tgcgcggccg cgaccttggc cagcagctcc gtcaacgtca
     7381 cgcgttcccg ctcgctcagc ggggccagca gccggtcgtt gtgctcggcg cccagcacac
     7441 gcagctgctc gcggacctgc tcgccggtcg gcgtcagccg cagcgcgtgc gagcgccggt
     7501 cactggggtg gcgctcgcgg cggaccaggc cgcggtcctc gaggtcgtcg acgaaggcga
     7561 ccacccggct cggcaggatg tccagccgct gcgcgagggc ctgctggctg gtgccggggt
     7621 cgtccgcgat cgcccgcagc acgccgacgt gcgggccggt gaggtccagt tcagcggcgc
     7681 gggcgaggaa ctgcgagctg gcgtgcgcgc cgagctggga cagcaggaac gcggccgtct
     7741 ccatgacccg agatgctaac cgtgcgcaca cagcgcccgg ctacgctgct gacgtgatcg
     7801 cccccaccga agcggacgcc gaaccggcgg acgttcccgc gccacgcagg cgagaccggc
     7861 tgcgcccgat gctggtcccg gcactgctct cggtgctcgg tggcgtgctg gcctacctgg
     7921 ccgaaccgcc acgcaccgtc tggtggctcg cgccgatcgg cctggcgttg ctcggctggg
     7981 tgttgcgcgg gcggcgcgcg aaggccgggt tcggctacgg cttcctgttc gggctcggct
     8041 acctggtgcc gctgctggcg tggaccggtg tgttcgtcgg accggtggcg tggctgccgc
     8101 tctccgcggt gtgtgcgttg ttgctggccc tggcgtgcgc ggcgatctcg gtggtgagca
     8161 agctgccggc gtggccgctg tgggcggcgt tgctgtgggt ggcgggggag acgttccgcg
     8221 cggtgttccc gttcggcggg ttcccgtggc cgaagctggc gttcgggcag cccgagggga
     8281 tctacctgcc gctggccgcg gtcggcggca cgccgttgat cgcgttcgcg gtgacgctga
     8341 ccgggttcgg gctggccgcg ttcgtgcgcg gtctcgccga cggtgaacgg accgtgggca
     8401 tgctggtggc ccgggcgctg gccgtgatga tcccggtcgc cgccgcgttc gcctcggcgc
     8461 cgctggtcgg caccgacgcc caggccggca cggtgaccgt ggccgcggtg cagggcaacg
     8521 tgccgcgcgc gggtctcgac ttcaacgccc agcgccgggc cgtgctggac aaccacgtgc
     8581 gccgcaccga gcagctggcc gcggacgtga aggccgggcg ggtgcccaag cccgacatcg
     8641 tgatctggcc ggagaactcc tccgacatcg acccgctgcg caaccccgac gcctacagcg
     8701 agatcaacca ggccgcgatc gacgtcggcg cgcccatcgc ggtcggggcc gtgctggtcg
     8761 gtgacgacgg caacccgcgc aacacggtgc tgctgtggga gccgggcaag ggcccggtcg
     8821 accggtacac caagcgccag ctgcagccct tcggcgagac gatgccgatg cgctcgttct
     8881 tccgcctgtt cacctcggcg gtggaccggg cgggcaactt cgtggccggc gacaagcccg
     8941 tggtgttcac catgggcaac gcccgggtgg cgttggacac gtgttacgag gtggccttcg
     9001 acggcgtggt gcgcgattcc atcgcgggct cgaccatcct cgccgtgccc acgaacaacg
     9061 ccaccttcgg ccgcacggag atgacatacc agcagctcgc gatggaccgc gtgcgtgcgg
     9121 tcgagcacgg gcgcgccgtg gtcgtcgcgg ccaccagcgg cgtgtccgcg ttggtgcgcc
     9181 cggacggttc ggtgatcgag cagacggagc tgttcacccc ggcagcgctg gtcgagcggc
     9241 tgccgctacg gacggcgact acgctgtcgg atcgactcgg tgcgacgccg gagtgggtga
     9301 tggatgccgc cgggctcctc gcgctcgccg ccgcggtcgc gttggggcgc cgcaggagcg
     9361 cctcaccgca ggagtgaacg atccccttgg tcgggggccg gcgcgggcag gcgccggtga
     9421 ggaggagaga cctacgcatg gcagagcagc cgagcaccgc agtggaaccg gtgctggtgg
     9481 tgatcccgac gtacaacgag cgggagaacc tgggaccgat cacacggcgt ttgcgcgacg
     9541 cactgccgaa ggcgcatgtg ctcgtggtgg acgacggcag ccccgacggc acgggtgagc
     9601 tggccgacga gctggccgcg aaggacgagc tggtgcacgt catgcaccgc acgtcgaagg
     9661 ccggcctggg cgcggcctac gtcgccggct tcgactgggc gctggaacgc gactacgcgg
     9721 tgatcgtgga gatggacgcc gacggctcgc acgccccgga ggacctgccg cggatgctgg
     9781 cggcgctcga ggacgccgat ctcgtgatcg gctcgcgcta cgtgcctggc gggcacacgg
     9841 tgaactggcc ggccagtcgc aagctcatct cctggggcgg gaacttctac tcgcggatgg
     9901 cgctgggcgc gaaggtcaag gacatcacgg ccgggttccg ggcctaccgg cggcaggtcc
     9961 tggagaagat caacctcggg aacgtggctt cggccgggta ctgcttccag atcgacctgg
    10021 cctggcggac gatcgaggcc gggttcaccg tggtcgaggt gccgatcacg ttcaccgagc
    10081 ggcaggtcgg cgactcgaag atgagcgggt cgatcgtgcg cgaggcgctg ctgcgagtga
    10141 cgaagtgggg gttgcggcgg cgggggactc agctgcgcaa cctcgttcgt cgctgacgct
    10201 tctggccacc atcccaacct tcggcgcctg gccagttcag ggcccggatg ctcaacctcg
    10261 tccgggcgga cgaggttgag ggggaggtct ggcttcgggg tgggcttggt ctgccatgga
    10321 accccaaatg catggggttg tcgggctgtg tgcccggagg ctctttctgg cgaccacgac
    10381 catggctgac cgggcttgtc tcgtggactc gccccggcgc cggagcgagg caagcccttg
    10441 aagggcggcc atgcgcgcga acgaagagag cgccatatac acagcaagaa ccccctggct
    10501 gggggttggc cagggggttc tcggtcttgg gggtgtctca ggcgctgcgg ctgcggcggc
    10561 cacggagctc ggccagccgt tcggtgagga gatcttccag ttcggggatc gtgcggcgct
    10621 cgagcagcat gtcccagtgg gtgcgcggtg gcttgctctt cttcggctcg gggtccgagc
    10681 cgtcgatcat cttggattcg aggccgtgga ggcggcattc ccagatcggc gggacctcgg
    10741 cgtcatcgga gaagggcacc tcgaagtcgt ggtccttcgg gcacgcgtat cggaccgtcc
    10801 ggcgtggggc aagatcgtg
//
