LOCUS       NZ_SNXZ01000006        21435 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Labedaea rhizosphaerae strain DSM 45361 Ga0310470_106, whole genome
            shotgun sequence.
ACCESSION   NZ_SNXZ01000006
VERSION     NZ_SNXZ01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Labedaea rhizosphaerae
  ORGANISM  Labedaea rhizosphaerae
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Labedaea.
REFERENCE   1  (bases 1 to 498874)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 498874)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004362825.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 13:11:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,820
            CDSs (total)                      :: 7,753
            Genes (coding)                    :: 7,690
            CDSs (with protein)               :: 7,690
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 11 of 63
            Pseudo Genes (incomplete)         :: 55 of 63
            Pseudo Genes (internal stop)      :: 3 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:41:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 363148
            Orig. end                         :: 384583
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SNXZ01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21435
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ranthipeptide"
                     /region_number="2"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /tool="antismash"
     cand_cluster    1..21435
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /kind="single"
                     /product="ranthipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21435
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11435](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11435)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
     gene            75..212
                     /locus_tag="EV186_RS39135"
     CDS             75..212
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EV186_RS39135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_166659399.1"
                     /transl_table=11
                     /translation="MVGLPLRDVHTGSVWVPVRPPTVPEHAPAVLVRAADIIDISRADQ
                     "
     gene            complement(193..861)
                     /gene="mftE"
                     /locus_tag="EV186_RS30710"
                     /old_locus_tag="EV186_106371"
     CDS             complement(193..861)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152862.1"
                     /locus_tag="EV186_RS30710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106371"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_243754371.1"
                     /transl_table=11
                     /translation="MRLDSTASVHGTLVVPVGSTEQHGPHLPFTVDTAIAAALAERLAA
                     EVSDVVIAPPVAYGASGEHAGFPGTLSIGVEATRVVLVELIRSADAFDGVVLVSGHGGN
                     AVPVRAACALAAREGRRVCVWSPTGPADDSHAGWTETSVMLALRPDAVRMDLAAPGVTT
                     PLPELMDRLRKDGVAGVSKSGVLGDPTKASAETGERILRQWTADLVRTVTAWRATDRRG
                     "
     gene            complement(858..2159)
                     /gene="mftF"
                     /locus_tag="EV186_RS30715"
                     /old_locus_tag="EV186_106372"
     CDS             complement(858..2159)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152863.1"
                     /locus_tag="EV186_RS30715"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106372"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_133852956.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.2e-15,
                     bitscore: 52.7, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLRLTRDGGTRVLRGGRVLVGGAPLRMLRLSKTGADLVSRWFA
                     GVLVDDTHRALARRLVQAGIAHPSYRDTGWSTADVTVVVPVRDHPVGVPGAVIVDDGSI
                     IPVSGAAVRHPQPRGPAAARNAGWRIATTELVAFVDADVRLTPGWLDPILAHFADPDVA
                     AVAPRVRSRAGTGLLARYEAVASSLDLGGEPAQVRPGGVVSYVPSATLVVRRSVLDRLG
                     GFDESMRFGEDVDLIWRMVDAGLSVRYEPTSTVEHAPRASWAGWVRQRFEYGTSAAPLA
                     VRHGRAVAPVRMSVWSALSWLAVAAGRPRTGIALALATAALLPVKLRRAGLPRGELVRL
                     AVTGHWYAGRLLADATTRSWWPISLALPWFRRGRWVLAAALARHLPGQGGIDPARWVLL
                     RAADDLAYGAGVWWGAVRDRTVRPLLPDFTEWRP"
     gene            complement(2156..2938)
                     /locus_tag="EV186_RS30720"
                     /old_locus_tag="EV186_106373"
     CDS             complement(2156..2938)
                     /NRPS_PKS="Domain: PKS_KR (3-177). E-value: 4.5e-08. Score:
                     25.2. Matches aSDomain:
                     nrpspksdomains_EV186_RS30720_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310355.1"
                     /locus_tag="EV186_RS30720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106373"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_133852957.1"
                     /transl_table=11
                     /translation="MSRVAVVTGAARGIGAAVVTRLAGAGWRVVAVDRCADLPGVGYPL
                     GTRADLDAVVAPWPSTVRAVVADVRSPHALVDAVALAERDFGGLDAAVAAAAVMAGGRP
                     LWETTDAEWDALFDTGVHGVLNLARAAVPALLRRPEPRAGRFVALASAAAHTGLWHLAG
                     YNAAKHAVVGLVRGLAADLRGTGVTATAVSPGSTDTAMLAATADLYGLSDVGEFATHQL
                     VDRLLTPDEVAAAVCWLCSAESGAVTGSVLHADGGFLA"
     aSDomain        complement(2408..2929)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EV186_RS30720_PKS_KR.1"
                     /evalue="4.50E-08"
                     /label="EV186_RS30720_PKS_KR.1"
                     /locus_tag="EV186_RS30720"
                     /protein_end="177"
                     /protein_start="3"
                     /score="25.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAVVTGAARGIGAAVVTRLAGAGWRVVAVDRCADLPGVGYPLGTR
                     ADLDAVVAPWPSTVRAVVADVRSPHALVDAVALAERDFGGLDAAVAAAAVMAGGRPLWE
                     TTDAEWDALFDTGVHGVLNLARAAVPALLRRPEPRAGRFVALASAAAHTGLWHLAGYNA
                     AKHAVVGLVRG"
     CDS_motif       complement(2861..2923)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EV186_RS30720_0001"
                     /evalue="4.00E-02"
                     /label="PKSI-KR_m1"
                     /locus_tag="EV186_RS30720"
                     /protein_end="26"
                     /protein_start="5"
                     /score="6.7"
                     /tool="antismash"
                     /translation="VVTGAARGIGAAVVTRLAGAG"
     gene            complement(2935..3741)
                     /locus_tag="EV186_RS30725"
                     /old_locus_tag="EV186_106374"
     CDS             complement(2935..3741)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04503.1"
                     /locus_tag="EV186_RS30725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106374"
                     /product="mycofactocin-associated electron transfer
                     flavoprotein beta subunit"
                     /protein_id="WP_166659400.1"
                     /transl_table=11
                     /translation="MLVIAALRWVDPRPGVDPLTGAVHTDPKASGPSAADECALAHALR
                     IAGPGRCVAVTAGPPAAEGLLRDALAAGATDALRIDDAGANTAVALTAGIRTLGPPDVI
                     VCGDHSLDHGTGATPALLAHAFGAAQALGLVELSMDGELTGLRRLDGGRRERLAIPLPA
                     VCSVEPTGLRPPRASLPAVLAAQAAAIPVVTVDSGPSMVQAGEQKPYRPRPTHLGAPAG
                     DAHHRVLALTGALTERTPPRVVRPASAAEAADELLDFLRKHGFLEP"
     gene            complement(3746..4036)
                     /locus_tag="EV186_RS39140"
                     /old_locus_tag="EV186_106375"
     CDS             complement(3746..4036)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310359.1"
                     /locus_tag="EV186_RS39140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106375"
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="WP_166659401.1"
                     /transl_table=11
                     /translation="MTYPERDFDDRMSTVEFRVGDRPHITVDQDVCRSCTTQACVHACP
                     ANLFVPTAEGGILFNYEQCFECGTCYQVCNQEGAITWTYPDGGHGVVFRKG"
     gene            complement(4033..5310)
                     /locus_tag="EV186_RS30730"
                     /old_locus_tag="EV186_106376"
     CDS             complement(4033..5310)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1119: halogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152867.1"
                     /locus_tag="EV186_RS30730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106376"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_133852959.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.3e-08, bitscore:
                     29.4, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 7.7e-10, bitscore: 33.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEFDVVVVGAGPAGSAAALEVARAGLSVALVERGPFPGAKNVFG
                     GVVYGRVLDELIPRWWERMPVQRWVTRRSTMVLTPTQALTVDLRAQDWGQAPYNGATAH
                     RSELDSWLAEQAVAAGATLITSTVVTGRSATGVRTDRPDGDIAAKVVIACDGVNSFLAK
                     EAGLFKAREHNTLGVKETLALPRAVIDERFGLTGDEGADFEIIGCTGDIPGGGFLYTNK
                     ESVGIGVVLELDGLTAAKVRPEELIAELKAHPAIAPLVRGGELVEYSAHLIPEGGYRTM
                     PELVTDGMLIAGDAAAMCLAAGIWLEGVNFALGAGMYAGRAAAAAIAKGDTSKAGLAAY
                     RTMLENSFVLGDHRRLRDMPGLVLSERVQRRYPGLVCDLAQGMFEVDNAQPKPGLRKLL
                     REARRTHGVRVRDLLRDGITAMRSLS"
     gene            complement(5303..6214)
                     /locus_tag="EV186_RS30735"
                     /old_locus_tag="EV186_106377"
     CDS             complement(5303..6214)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152868.1"
                     /locus_tag="EV186_RS30735"
                     /note="Built with help from friend_finder, bracket5, and
                     grep mycofactocin; Built with help from friend_finder,
                     bracket5, and grep mycofactocin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="EV186_106377"
                     /product="mycofactocin-associated electron transfer
                     flavoprotein alpha subunit"
                     /protein_id="WP_243754372.1"
                     /transl_table=11
                     /translation="MIGAPGRPLALLVVRDGEPPLGADEAVAEAAGAALVVGSGPSATG
                     LVSARRLWLAETGSFAPGALAAALSRLTTCGPIILPASPDGRDLAPRLAAVLGRPLLAN
                     AVRVHDHGAEVTRWGGQVTVDLTAAGPFVATLVPGVRGSDPVGAPPAVEHVDIKVERGD
                     DVEVLETLEADPATADLAEAPRILGAGAGLGSRGHTALLGKVAAALGASLGATRVVTDA
                     GWAPYERQIGTTGVAVDPELYVAFGVSGAAQHLGGLGSPRAVVSVNTDPSCPMTAMADL
                     GIVTDAPGLLDELAERLGVERD"
     gene            complement(6211..8169)
                     /locus_tag="EV186_RS30740"
                     /old_locus_tag="EV186_106378"
     CDS             complement(6211..8169)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152869.1"
                     /locus_tag="EV186_RS30740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106378"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="WP_243754373.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.2e-12, bitscore:
                     41.2, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRLRYLFSPLRVGPLVLRNRIVFAAHLTNYAVDGLPSEQHAAYY
                     AARAAGGAGLIITEEHSTHPTDWPYEKLIHGHHPQVVPGYRRITEAVHRHDTPILAQLN
                     HNGGQASSMYSRLPVWAPSAVPDPMFREVPKAIDTREIAEVVAGYATVAGHCAEGGFDG
                     VELQCSHSSIVRGFLSPTTNVRTDRYGGPLANRARILLEIVAAVRAAIGPDRVLGVRIC
                     GDELIEGGTTIDEAVEVARMVEASGQVDYLNTSIGVATASLFMIEASMAVPPGYALFIA
                     NAMRKAVRLPVVGVGRIKDPVQAERALAAGHCDLVGVVRGQIADADFAAKARSGHGTEI
                     RTCLSCNQECVGRMGLNRWLGCIENPRTGREAIDLPMPRRRDLRVLVVGGGPGGLQAAS
                     TAAQRGHHVTLFERAEDVGGQVAVAARVPGRAEFLDVVRNLRAECQRYGVDVKTGTEAT
                     ASLLLAESPDAVVLATGARPLRPYWAGSSARVVDVRDVLEQRCAPSGRVLVVDDLGFHQ
                     ATSVAELLADRGCSVRISTSGMVVGQDLGITLDMEMFCVRAHAKGIELTTDEVITGVAE
                     GVPAEGVTVQLLKHTTSETFDLPVDWVVCATHQEPEDELWHALRDAPFAVHRVGDCVTP
                     RRAHAAVVEGHRAALAL"
     gene            complement(8166..10004)
                     /locus_tag="EV186_RS30745"
                     /old_locus_tag="EV186_106379"
     CDS             complement(8166..10004)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152870.1"
                     /locus_tag="EV186_RS30745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106379"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 1"
                     /protein_id="WP_133852960.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.7e-09, bitscore:
                     31.6, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITDPVTLAGRQAPSRVLFGPHETNLGRGRAFSDRHVAYYARRAR
                     GGAGVIVTETASVHPSDWPYERAPLAAECGPGWAAIASACQGSLVLASLGHSGSQGSPA
                     FGQQALWAPSRVPDVASRELPMAMEQAEIDALIAGFGAATKLAMASGVDGVELEAGQFS
                     LLRQFCSGLTNQRDDRYGDKSTLLHEVIAVVRASVGDGVLGLRLSCDELAPWAGITPDQ
                     GASLAASVADAVDYLVVVRGSAMGTSATRPDFHTPPGFNIELCRQVRAAVAGRCPVVLQ
                     GSVIDPAMAEEALDGVAELVEMTRAQIAEPDLVALWRAGHGARIRPCVLCNQRCRVRDN
                     RNPIVSCVGEPASGHETEDPPITPAAAPRDVLVVGGGPAGLEAARVLALRGHRVELVER
                     SDVLGGMLRHAPGRFAALADWLAAEIRRLGVTVRTGATADPGDRLVVRAVGSVPGPRAY
                     DVAEGAVVVEAVDVLSGAELPPGAVAVLDPVGDAVGVAVAERLAGAGRSVAIVSQDQVI
                     GTQLALTGDLADANARLQRAGVRLVKRSKPRAVRPGSVLVEDVFTAEQSNVPAEVGVHC
                     GHRLPADADGVTAGDCVAPRTVYEAILEGRRAGALP"
     gene            complement(10001..11140)
                     /gene="mftC"
                     /locus_tag="EV186_RS30750"
                     /old_locus_tag="EV186_106380"
     CDS             complement(10001..11140)
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076819.1"
                     /locus_tag="EV186_RS30750"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="EV186_106380"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_133852961.1"
                     /sec_met_domain="PTHR43273 (E-value: 1.4e-37, bitscore:
                     124.4, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 3.3e-07, bitscore: 25.7,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 3.7e-23, bitscore: 77.7,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 6.5e-09, bitscore: 30.5,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 5e-14, bitscore: 47.5,
                     seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.2e-07, bitscore: 26.3,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLVDQFKKGLSAPICLTWEWTYACNLSCTHCLSSSGRRDPKELT
                     TAEIKAVIDELQRMQVFYVNIGGGEPTVRPDFWELMHYAVTHDVGVKFSTNGLRITPLR
                     ARQLVATDYVDVQISLDGATREVNDAVRGPGSYDTAIAAMANLADAGFAGFKVSVVMTR
                     QNVPQLGEFKAIADRYGAQLRITRLRPSGRGADVWDDLHPTQGQQRQIYDWLVRHGDNV
                     LTGDSFFHLNALGSTSLPGLNLCGAGRVVCLIDPVGDVYACPFAIHDAFLAGNVREAGF
                     GRVWRESPLFAQLREPQSAGACASCGEYDACQGGCMAAKFFTGLPLAGPDPECVKGNAP
                     TTGLAIPAQGKDHSHRKRVALGMPSVPAKPCDPSPLAVR"
     gene            complement(11160..11435)
                     /gene="mftB"
                     /locus_tag="EV186_RS30755"
                     /old_locus_tag="EV186_106381"
     CDS             complement(11160..11435)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229645.1"
                     /locus_tag="EV186_RS30755"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="EV186_106381"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_133852962.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 9.8e-27,
                     bitscore: 88.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTDSPGFDPAAPYRLNPRVALRPEPFGALAYHFGTRKLSFLKAP
                     ALVELVRDLEAHPTVLAAVDHLAAPEKDRYLRALATLAETDMIVPA"
     aSDomain        complement(11172..11402)
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_EV186_RS30755_Mycofactocin_RRE.1"
                     /evalue="5.40E-28"
                     /identifier="RREFam016.1"
                     /label="EV186_RS30755"
                     /locus_tag="EV186_RS30755"
                     /protein_end="88"
                     /protein_start="11"
                     /score="88.1"
                     /tool="antismash"
                     /translation="APYRLNPRVALRPEPFGALAYHFGTRKLSFLKAPALVELVRDLEA
                     HPTVLAAVDHLAAPEKDRYLRALATLAETDMI"
     gene            complement(11425..11505)
                     /gene="mftA"
                     /locus_tag="EV186_RS30760"
     CDS             complement(11425..11505)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947188.1"
                     /locus_tag="EV186_RS30760"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_133853125.1"
                     /transl_table=11
                     /translation="MDEEPVEVEEELLVEDVSIDGMCGVY"
     gene            11579..12193
                     /gene="mftR"
                     /locus_tag="EV186_RS30765"
                     /old_locus_tag="EV186_106382"
     CDS             11579..12193
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="mftR"
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421041.1"
                     /locus_tag="EV186_RS30765"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from the
                     terminal Val-Tyr dipeptide of the precursor peptide MftA,
                     through a radical SAM enzyme-mediated process.; MftR, the
                     mycofactocin system transcriptional regulator, is an
                     uncharacterized TetR family DNA-binding transcription
                     factor. Its role is inferred by context. It occurs as part
                     of the biosynthesis locus for mycofactocin, a partially
                     characterized electron carrier derived from the terminal
                     Val-Tyr dipeptide of the precursor peptide MftA, through a
                     radical SAM enzyme-mediated process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="EV186_106382"
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="WP_243754374.1"
                     /transl_table=11
                     /translation="MYGNDSATPARGGRKPVTSRAELEHVAFDLFAAQGFEATTVDDIA
                     RAAGIGRRTFFRYFPSKNDIPWGAFDEQLDQLRTDLRAYPVDQPLMDVVRAALVDFNRV
                     PAAEVPWHRRRMALILQVPALQAHSTLRYANWRQVVAEFVAERLDQPVDALGPQTIAYA
                     TLGVALASYEQWLRDESQDLAALMAAAMTGLAESFTGVRTT"
     gene            complement(12332..12730)
                     /locus_tag="EV186_RS30770"
                     /old_locus_tag="EV186_106383"
     CDS             complement(12332..12730)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /locus_tag="EV186_RS30770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106383"
                     /product="DUF1707 domain-containing protein"
                     /protein_id="WP_133852964.1"
                     /transl_table=11
                     /translation="MAGSANIRVGSAERESALHALAEHLTADRLDVAEYEQRCAAVTAA
                     RTRADLLAVFDDLPVPHPKWDPEELEPATAPEQSPLIDNSPARLSRPIVLALGAIMIVA
                     AVVVAAVTTTWWALLPALLIIIVMFLTN"
     gene            complement(12762..13766)
                     /locus_tag="EV186_RS30775"
                     /old_locus_tag="EV186_106384"
     CDS             complement(12762..13766)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /locus_tag="EV186_RS30775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106384"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_133852965.1"
                     /transl_table=11
                     /translation="MPLTKIALEEHFLDPRAAADVLADPDKLQRLSDGGGVMPEYYRPI
                     LGLLGDFDDQRLSTMDKFGIECAVLSLTAPGIQLVEGTADAIAMARDANDLLAEQVRKH
                     PDRYRGFASVALQDPAAAAEELRRCVGELGFVGAMVNGYTATGDGGDAYLDDERFTGFW
                     EAVADLGVPVYLHPRPAPPTDRHAIAGHPQLVGATWGFGVETATHTLRLIFSGLFDRFP
                     GVQLVLGHLGEGLPAQLWRTQYCYDLNPFDKRIEKTLPEYFADNIHVTTSGNFSDQALI
                     TALLTVGGDRVLFSVDYPYADTELATTWLDRAPISESDRRKIAGGNARRLFGL"
     gene            complement(13788..14327)
                     /locus_tag="EV186_RS30780"
                     /old_locus_tag="EV186_106385"
     CDS             complement(13788..14327)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818701.1"
                     /locus_tag="EV186_RS30780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106385"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_133852966.1"
                     /transl_table=11
                     /translation="MTEVSFTLFDTPIGRCSIAWGANGVVGVGLPEPTDLRAEVRMRRR
                     WPDVRRDKPPQPLADAITRIAALLGGEKSDLSDVEVDWSEVTEFDRSVYEVARTVPPGK
                     TITYGEIAKQLGDPLAARAVGQAMGNNPYPLVVPCHRVLAAGGKLGGFSATGGVETKRR
                     ILVIEGALEPNLFDEF"
     gene            14443..15312
                     /locus_tag="EV186_RS30785"
                     /old_locus_tag="EV186_106386"
     CDS             14443..15312
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447755.1"
                     /locus_tag="EV186_RS30785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106386"
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="WP_133852967.1"
                     /transl_table=11
                     /translation="MGEVSRVGVVGSGLMGSGIAEVCARAGLDVLVNEVNQDASDAGRA
                     RIEKSLARGVRSGKLAEDDRDAALARLSFTTDLGDFADRDIVIEAVAEHEATKVDVFRT
                     LDKVVRREDALFASNTSSIPIMKLGMATSRPAQVIGVHFFNPVPVLPLVELVPSLLTSD
                     ETRAAAEEFVTGPLGKQVIQSQDRAGFVVNALLIPYLLSAIRMMESGFASAEDIDQGIV
                     LGLAHPMGPLRLTDLIGLDTTKAIAESMYAEFKEPLYSPPPLLLRMVDAGLLGKKSGRG
                     FYDYEVAK"
     gene            15309..16460
                     /locus_tag="EV186_RS30790"
                     /old_locus_tag="EV186_106387"
     CDS             15309..16460
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643504.1"
                     /locus_tag="EV186_RS30790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106387"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_133852968.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.9e-30, bitscore:
                     99.8, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 8.1e-31, bitscore:
                     101.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.6e-50, bitscore:
                     165.5, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLYQLGEEHEELRAAIRALVDKEIAPHAAAVDEDARFPEEARK
                     ALVAADMHAVHIPEFYGGQGADAVAACIVIEEVARACASSSLIPAVNKLGTVPLLLSAS
                     EELKSQVLPGIAKGEMASYALSEREAGSDTASMRTRARLDGDHWVLNGTKAWITNAGES
                     TWYTVMAVTDPNAAKPANGISAFVVHKDDPGFSVGPKERKLGIKGSPTREIHFEDCTIP
                     ADRIVGEPGTGLKTALATLDHTRPTIGAQAVGIAQGALEASIAYVKERKQFGKAIAEFQ
                     GLQFMLADMGMKVEAARHLVYAAAAKAERGDPGAGFTASAAKTFASDTAMQVTTDAVQL
                     FGGAGYTRDFPVERMMRDAKITQIYEGTNQIQRVVMARQLLKG"
     misc_feature    complement(16474..16488)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.82"
                     /tool="antismash"
     gene            16584..18557
                     /locus_tag="EV186_RS30795"
                     /old_locus_tag="EV186_106388"
     CDS             16584..18557
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662006.1"
                     /locus_tag="EV186_RS30795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106388"
                     /product="S53 family peptidase"
                     /protein_id="WP_243754375.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 6.6e-09, bitscore:
                     30.4, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGQYRKRWVAGATATLLAAMTVVLGHDASAAPSDRRVLAGSVPPW
                     AAAANRASNAPDTDSVGFRVYLGWQNQSGAEALAARVSTPGSADYGKFVSPAQFRAQFA
                     PSPSDASAVQQWLRKSGFDISTTPGNNRYVQAEGTVAQAKAAFGAGFGEYRVAGKTLRA
                     PDGALSVPADLPASVTSVVGLDESAALTVPSAPPSPVFVNAAPCSAYWNEKNTATTLTP
                     NGTKAPDAYGHPNPWAPCGYTPAQLRSAYGMTSSAANGSGQTVAIIDAYASPTIVQDVN
                     TYSSLHGLPALNGSNFSQVTPPGVFKRPQNSHQDPQGWYGEETLDVEAVHGMAPGAKIV
                     YVGSPNNYQDLDAAMNHVVDQHLASIVTNSYGFSTELLPRGYVKPLNDTLIEAAATGVG
                     VYFSSGDNGDETAGDPANAASATPDWPAVSPWVTAVGGTAIAIGASGNYLFEAGWETGR
                     SNLVNGAWSPTPPGNYTSGSGGGTSRLFTQPAYQAGVVPNAIATANGARPQPMRVVPDV
                     ASIADPNTGMLVGQTQTFPDGHAAYSEYRIGGTSLASPVFAGMVAVAQQQAGRTFGFAN
                     PLFYRNAGTSAFHDIVPPAAPVAVARNDFANTVDSSEGYVVSLRTLDFDANLTIHTRPG
                     YDDVTGIGSPNGAAWLSALAAG"
     gene            complement(18754..19683)
                     /locus_tag="EV186_RS30800"
                     /old_locus_tag="EV186_106389"
     CDS             complement(18754..19683)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EV186_RS30800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EV186_106389"
                     /product="hypothetical protein"
                     /protein_id="WP_133852969.1"
                     /transl_table=11
                     /translation="MEYQLTALLVPPPDTAELDPLQRHGVAALLDEQLDMLAGIEGPDG
                     VEIEPLDHRIDVTTTGAHIEWLVEAPALAFAEEGAKHVLDELLAQTELLARWQVQRCEV
                     TASDAALEAAMSDEPPQDEEEDELTEADLEEHLDQLADAGGALRAFEFDDLAEEHAQLL
                     AGALITSAEILVGELFGDIAALEDGDTTVDEHEELAVLPLLPERYRDRYTAHFARQFLI
                     TMVIVGYRLTQPAWIPPGTIAESLALHLLRMEAATQLAVHEDLPTEEMLAAFDAVAVHE
                     VSHEHLYKPHPESVDEDDAAADPDLWWD"
     gene            complement(19719..21116)
                     /locus_tag="EV186_RS30805"
                     /old_locus_tag="EV186_106390"
     CDS             complement(19719..21116)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804009.1"
                     /locus_tag="EV186_RS30805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV186_106390"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_133852970.1"
                     /transl_table=11
                     /translation="MTGVDPDRLRECLAVLAEVELLPTDHPDAVVVRRATSRMYKVARQ
                     RKKAAKRAAITEADREVIARTATGSPDRIDDETKGIPLVSTVPGASAGEYQRARSCYTC
                     KKRYTQVDAFYHQLCPPCAARNRDRRDARVDLTGRRALLTGGRAKIGMYIALRLLRDGA
                     HTTITTRFPKDAIRRFSAMDDSAEWLHRLRVVGIDLRDPAQVVALADSVAEQGPLDILI
                     NNAAQTVRRSSGAYAELIAAESGPLPAGELPEMITFGRTAEEHPIALASVPVTELALVA
                     KSASPDRVAAGTAIDAGGLVPDLAATNSWVDRVHEVDPTELLEVQLCNQTAPFILVSRL
                     RAAMLASPARRKYVVNVSAMEGQFSRAYKGAGHPHTNMAKAALNMLTRTSAFDLVDDGI
                     LMTAVDTGWITDERPHPTKMRLADEGFHAPLDLVDGAARVYDPIVRGEAGEDLYGCFLK
                     DFEPAPW"
     misc_feature    21422..21435
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.64"
                     /tool="antismash"
ORIGIN
        1 gtacggtcac ctacacgaca cgtgcgggtg cccgtcgcag acatccgccg accgcgccga
       61 tcaccaccgc ggtcgtggtg gggctgcccc tgcgcgacgt ccacaccgga tcggtctggg
      121 tcccggtccg tccgccgacc gtgcccgagc acgcgccggc ggtgctggtg cgtgccgccg
      181 acatcatcga catcagccgc gccgatcagt agcccgccag gcggtgaccg tccggaccag
      241 gtcggcggtc cactggcgca ggatgcgttc cccggtctcg gcgctggcct tcgtcgggtc
      301 gccgaggacg ccggacttgc tcacgcccgc gacaccgtcc ttgcgcaggc ggtccatcaa
      361 ctcgggcaag ggcgtggtca cccctggcgc ggccaggtcc atccgcaccg cgtccggccg
      421 cagggccagc atcaccgacg tctccgtcca gcccgcgtgg ctgtcgtcgg cgggccccgt
      481 cggtgaccac acgcataccc gcctgccttc gcgcgcggcg agcgcacacg cggcacgcac
      541 gggtaccgcg ttgccgccgt ggccggaaac cagcaccaca ccgtcgaaag cgtcggccga
      601 gcggatcagc tccaccaaca ccacccgggt cgcctcgacg ccgatggaca acgtcccggg
      661 gaacccggcg tgctcgccgc tcgccccgta ggccaccggc ggtgcgatga ccacatcgga
      721 cacttccgcc gcgagccgct cggcgagggc cgccgcgatc gcggtgtcca cagtgaacgg
      781 gaggtgcggg ccgtgctgct cggtcgagcc caccgggacc acgagcgtgc cgtgcaccga
      841 cgccgtcgag tccagcctca cggccgccac tccgtgaagt ccggcagcag cggccgcacc
      901 gtgcgatcgc gcaccgcgcc ccaccacacc ccggcgccgt aggccaggtc gtcggccgcg
      961 cgcagcagca cccagcgcgc cgggtcgatg ccgccctggc cgggaaggtg ccgggccaag
     1021 gctgctgcca gcacccaccg gccacgccgg aaccacggca acgccaagga aatcggccac
     1081 cacgagcgcg ttgtggcatc ggcgagcagg cggccggcgt accagtggcc cgtcaccgcg
     1141 agccggacca gttcgccgcg gggcagcccg gcccggcgca gtttgaccgg caacagcgcg
     1201 gctgttgcca acgccagcgc gattcccgtg cgtggccgtc cggcagcgac cgccagccac
     1261 gacagcgcgc tccacaccga cattcgcacc ggcgccaccg ctcgcccgtg ccgcacggcc
     1321 agcggtgctg ccgatgtccc gtactcgaac cgctgccgca cccagccggc ccagctggcc
     1381 cgcggcgcgt gctccacagt ggacgtgggc tcgtagcgca ccgacaaccc ggcatcgacc
     1441 atgcgccaga tcaggtcgac gtcctcgccg aaccgcatcg actcgtcgaa gccgcccagc
     1501 cggtccagca ccgatcgccg caccaccagg gtcgccgacg gcacgtagct gaccacccca
     1561 ccgggccgca cctgagccgg ctcaccgccg aggtcgaggc tggacgcgac cgcctcgtac
     1621 cgggcgagca agcccgtgcc cgcgcggctg cgcacgcgcg gcgccacggc ggcgacgtcc
     1681 ggatcggcga agtgcgccag gatcgggtcg agccagccgg gcgtgagccg cacatcggca
     1741 tccacgaagg cgaccagctc ggtggtggcg atccgccacc cggcgttgcg tgccgcagcc
     1801 gggccgcggg gttgcgggtg ccggacggcg gcaccggaca ccgggatgat cgacccgtcg
     1861 tccacgatca ccgcgccggg cacgcccacc gggtggtcgc gcaccggcac gaccacggtg
     1921 acgtccgcgg tgctccaccc ggtgtcgcga taggacggat gagcgatccc ggcctggacc
     1981 agccgccggg ccagcgcccg gtgcgtgtcg tcgaccagca cacccgcgaa ccaccggctc
     2041 accaggtcgg cgcccgtctt ggacaggcgg agcatgcgca ggggagcgcc accgacgagc
     2101 acccggccgc cgcgcagcac ccgggtgccg ccgtcacggg tcaaccgcag cgtggtcatg
     2161 ccaggaaccc gccgtcggcg tgcagcacgc tgccggtgac cgcgccggac tccgcggagc
     2221 acagccagca caccgcggcg gccacttcgt cgggcgtcag caagcgatcc acgagctggt
     2281 gcgtggcgaa ctcgcccaca tcggacagtc catagaggtc ggcggtcgcg gccagcatgg
     2341 cggtgtcggt cgaacccggt gacacggcgg tcgcggtcac gccggtgccc cgcaagtccg
     2401 cggccaggcc gcggaccagc ccgaccacgg cgtgcttggc cgcgttgtag ccggcgaggt
     2461 gccacaggcc ggtgtgtgcg gccgccgacg ccagggccac gaaccgcccg gcccgcggct
     2521 ccgggcggcg caacaaggcg ggtaccgcgg cccgtgccag gttcagcacg ccgtggaccc
     2581 cggtgtcgaa caacgcgtcc cactcggcgt cggtggtctc ccacaacggg cggccaccgg
     2641 ccatgacggc agccgccgcc acggccgcgt ccaggccgcc gaagtcgcgt tcggccaagg
     2701 ccacggcgtc gaccaaagcg tgcggggaac gcacgtcggc gaccaccgcg cgcaccgtcg
     2761 acggccaggg cgcgaccacg gcgtccaggt ccgcccgggt gccgagcggg taacccacgc
     2821 cgggcagatc ggcgcagcgg tcgacggcca cgacccgcca ccccgcaccg gccaggcggg
     2881 tgaccacggc ggcgccgatc ccccgcgccg ccccggtcac caccgcgacc cggctcatgg
     2941 ctcaaggaaa ccatgcttgc gcaggaagtc gagcagttcg tcggcggctt cggcagccga
     3001 cgccgggcgc acgacgcgag gtggcgtccg ctcggtcagc gccccggtca acgcgagcac
     3061 ccggtggtgg gcgtcgccgg ccggtgcgcc gaggtgggtc gggcggggcc ggtagggctt
     3121 ctgctcgcct gcctggacca ttgacggtcc actgtcgacg gtcacaacgg ggattgccgc
     3181 ggcctgggcg gcgagcaccg cgggcagcga ggcccgcggc ggccgcagcc cggtcggctc
     3241 caccgaacac accgcgggca gtgggatggc cagccgctcg cggcgcccac cgtcgagccg
     3301 gcgcaggccg gtgagctcgc catccatgga cagttcgacc agcccgaggg cctgagcggc
     3361 accgaaggcg tgggcgagca gggccggcgt cgcgccggtg ccgtggtcga gcgagtggtc
     3421 gccgcagacg atcacgtccg gcgggccgag cgtgcggatg cccgcggtca gcgcgaccgc
     3481 cgtgttggcg ccggcgtcgt cgatgcgcag ggcgtcggtg gccccggcgg cgagcgcgtc
     3541 acgcagcagc ccttcggccg ccggcggccc ggccgtgacg gcgacgcacc ggcccggccc
     3601 ggcaatgcgc agggcgtggg cgagcgcgca ctcgtcggcc gcgctcgggc cgctggcctt
     3661 cgggtcggtg tgcacggcgc ccgtcagcgg gtccacgccc gggcgcggat cgacccagcg
     3721 cagggcggcg atcaccagca ccggctcacc ccttccggaa cacgacgccg tggccgccgt
     3781 ccgggtaggt ccaggtgatc gcgccctcct ggttgcacac ctggtagcac gtgccgcact
     3841 cgaagcactg ctcgtagttg aacaggatgc cgccctcggc cgtcggcacg aagaggttcg
     3901 ccgggcacgc gtgcacgcag gcctgcgtgg tgcacgagcg gcacacgtcc tggtcgacgg
     3961 tgatgtgcgg ccggtcgccg acccggaact ccacagtgga catacggtcg tcgaagtcgc
     4021 gctccgggta ggtcatgaca gcgacctcat cgcggtgatc ccgtccctga gcaggtcgcg
     4081 cacgcgcacg ccgtgggtcc gccgtgcctc gcgcagcagc ttgcgcagcc cgggcttcgg
     4141 ctgcgcgttg tccacttcga acatcccctg cgcgaggtcg cagaccaggc cggggtagcg
     4201 ccgttgcacc cgttcggaca gcacgaggcc gggcatgtcg cgcagcctgc ggtgatcacc
     4261 gaggacgaag gaattctcga gcatggtgcg gtaggcggcg agtccggcct tggacgtgtc
     4321 gcccttggcg atcgccgccg cggcggcccg gccggcgtac atgcccgcgc cgagggcgaa
     4381 gttcacgccc tccagccaga tccccgcggc gaggcacatc gcggccgcat cccccgcgat
     4441 gagcatgccg tcggtcacca actcgggcat cgtgcggtag ccgccctcgg ggatgaggtg
     4501 cgccgagtac tcgaccagct cgccgccgcg caccagcggc gcgatggcgg ggtgcgcctt
     4561 cagctcggcg atcagctcct cgggacgcac cttggcggcc gtgagcccgt cgagttcgag
     4621 cacgacgccg atcccgacgg attccttgtt ggtgtacagg aatccgccgc cggggatgtc
     4681 gccggtgcag ccgatgatct cgaagtccgc gccctcgtca cccgtcaacc cgaaccgctc
     4741 gtcgatcacg gcccgcggca gcgcgagcgt ctccttcacg ccgagcgtgt tgtgctcccg
     4801 agccttgaag agcccggctt ccttggccag gaaggaattc accccgtcgc aggcgatcac
     4861 gaccttggcc gcgatgtcgc cgtccggccg gtcggtccgc accccggtcg ccgaccggcc
     4921 ggtgaccacg gtggacgtga tgagcgtggc gcccgcggcc acggcctgct cggccagcca
     4981 actgtccagt tcggaccgat gggcggtggc gccgttgtag ggggcctggc cccagtcctg
     5041 tgcccgcagg tcgacggtca acgcctgggt cggcgtcagg accatggtgg agcggcgggt
     5101 gacccagcgt tggacgggca tccgttccca ccagcggggg atgagttcgt cgagcacccg
     5161 gccgtagacg accccgccga agacgttctt ggccccgggg aacggcccac gctcgaccaa
     5221 cgccaccgag agcccggccc gcgccacctc cagcgcggcc gccgagccgg ccggacccgc
     5281 gccgaccacc accacgtcga actcagtcac gctcgacccc cagccgttcg gccagctcgt
     5341 cgagcagccc cggcgcgtcg gtgacgatgc cgagatcggc catggcggtc atggggcagg
     5401 acggatcggt gttcacgctg accacggcgc ggggcgagcc gagcccgccg aggtgctgcg
     5461 ccgcgccgga caccccgaac gccacgtaga gctcgggatc gaccgcgacg ccggtggtgc
     5521 cgatctgccg ttcgtagggc gcccacccgg cgtcggtgac cacgcgcgtg gcccccagcg
     5581 acgcgccgag cgcggccgcg accttgccca gcaacgcggt gtggccgcgg ctgccgagtc
     5641 cggcaccggc cccgaggatg cgcggcgcct cggcgagatc ggcggtggcc ggatcggctt
     5701 cgagcgtctc gagtacctcg acgtcgtcgc cccgctcgac cttgatgtcc acatgttcga
     5761 cggccggtgg tgcgccgacc ggatcgctcc cacgcacgcc gggcaccaac gtggccacga
     5821 agggcccggc ggcggtcaag tcgacggtga cctgcccgcc ccaccgcgtg acctcggcgc
     5881 cgtggtcgtg cacgcgcacc gcattggcca gcaggggccg tccgagcacg gcggccagcc
     5941 tcggcgccag gtcgcgaccg tcgggggagg ccggcaggat gatcggcccg cacgtcgtga
     6001 gcctgctcaa cgccgcggcc aatgccccgg gcgcgaacga gccggtctcc gcgagccaca
     6061 accggcgagc cgagaccagc ccggtggcgc tcggcccgga cccgaccacc agcgccgcgc
     6121 cggcggcctc ggccaccgcc tcgtccgcac ccagcggcgg ctccccgtcg cgaacgacga
     6181 gaagggcgag tggccggccg ggggcgccga tcacaaggcc aacgccgctc ggtggccttc
     6241 gacgaccgcg gcatgggcgc gcctcggcgt cacgcagtcg ccgacccggt ggacggcgaa
     6301 cggggcatca cgcagggcgt gccacagttc gtcctccggc tcctggtggg tcgcgcagac
     6361 cacccagtcc accggcaagt cgaaggtctc gctcgtcgtg tgcttgagca actgtacggt
     6421 cacaccctcg gccggcacac cctcggccac gccggtgatc acctcgtcgg tggtcagctc
     6481 gatgcccttc gcgtgggcgc ggacgcagaa catctccatg tccagcgtga tccccaggtc
     6541 ctggccgacg accatcccgg acgtgctgat ccgcaccgag cagccacggt cggccagcaa
     6601 ctccgccacg ctggtcgcct ggtggaagcc caggtcgtcc accacgagca cgcgtccgga
     6661 cggggcgcag cgctgctcga gcacgtcccg cacatccacg acgcgcgcac tcgacccggc
     6721 ccagtacggg cgcagcgggc gagcgcccgt cgccagcacg acggcatccg gggactcggc
     6781 cagcagcagc gacgccgtcg cctcggtgcc ggtcttcacg tccacgccgt agcgctggca
     6841 ttccgcgcgc agattccgca ccacgtcgag gaattcggcc cggccgggga cgcgcgcggc
     6901 caccgcgacc tgcccgccga catcctcggc ccgttcgaac aacgtcacgt ggtgcccgcg
     6961 ttgcgcggcc gtcgacgccg cctgcaggcc acccggacca ccgccgacga cgagcacccg
     7021 gagatctcgg cgccggggca tcggcaggtc gatggcctcg cgcccggtgc gggggttctc
     7081 gatgcagccg agccagcggt tcaggcccat ccggccgacg cattcctggt tgcaggacaa
     7141 gcaagtccgg atctcggtgc cgtgcccgga ccgcgccttg gccgcgaagt ccgcgtccgc
     7201 gatctgcccg cgcacgaccc ccaccaggtc gcagtgcccg gccgcgagcg cgcgctcggc
     7261 ctgcacggga tccttgatcc ggccgacgcc gacgaccggc aggcgcacgg ccttgcgcat
     7321 ggcgttggcg atgaacaagg catagccggg cgggacggcc atggacgcct cgatcatgaa
     7381 caggctcgcg gtagcgacac cgatcgacgt gttcaggtag tcgacctgcc cgctggcctc
     7441 aaccatgcgc gcgacctcga cggcctcgtc gatcgtcgtg ccaccctcga tcagttcgtc
     7501 accgcagatg cgcacgccca gaacgcggtc cggcccaatc gccgcgcgca ccgcggccac
     7561 gatctccagc aggatgcgcg cccggttggc cagcggaccg ccgtagcggt cggtgcgcac
     7621 attcgttgtg ggagaaagga agccacgcac gatcgacgag tgcgagcact gcagttccac
     7681 gccgtcgaag ccgccttcgg cgcaatgccc ggcgaccgtc gcgtagccgg cgaccacctc
     7741 ggcgatctcg cgggtgtcga tggccttggg cacctcgcgg aacatcgggt ccggcaccgc
     7801 gctcggcgcc cacaccggca accgcgagta catcgacgag gcctgcccgc cgttgtggtt
     7861 gagctgggcc aggatcggcg tgtcgtgccg gtgtaccgcc tcggtgatcc gccggtaacc
     7921 ggggacgacc tgcgggtggt ggccgtggat cagcttctcg tacggccagt ccgtcgggtg
     7981 ggtcgagtgc tcctcggtga tgatcaaccc ggcgcctccg gccgcgcggg ccgcgtagta
     8041 ggccgcgtgc tgctcggacg gcaggccgtc gaccgcgtag ttggtcaggt gcgcggcgaa
     8101 cacgatccgg ttgcgcagca ccagcgggcc cacccgcaac ggcgagaaca ggtaacgcaa
     8161 gcggctcacg gcaacgcacc ggcccgcctg ccttcgagga tcgcctcgta gacggtcctc
     8221 ggcgcaacgc agtcacccgc ggtgacgccg tcggcgtcgg cgggcaatcg atgtccacag
     8281 tggaccccaa cctcggcggg cacgttgctc tgctcggccg tgaacacgtc ctccaccagt
     8341 accgaaccag ggcgcacggc ccggggcttg ctgcgcttca ccaggcgcac acccgcgcgc
     8401 tgcagccgcg cgttcgcgtc ggccagatca ccggtgagcg cgagctgggt gccgatcacc
     8461 tggtcctggc tgacgatcgc gaccgaacgt ccagcccccg caaggcgttc ggccaccgcc
     8521 acgcccaccg cgtcgccgac cgggtcgagc accgcgaccg cgcccggcgg caactccgca
     8581 ccgctcagca cgtccaccgc ttccaccacg accgcgccct cggccacgtc gtaagcccgt
     8641 gggccgggca ccgacccgac cgctcgcacc accagccggt cgcccgggtc cgcggtggcg
     8701 ccggtccgca cggtgacgcc cagccgccgg atctcggccg ccaaccagtc ggcgagcgcg
     8761 gcgaaccggc ccggcgcgtg ccgcagcatc ccgccgagca cgtccgaacg ctcgaccagt
     8821 tccacccggt gcccccgcaa cgcgagcacc cgagccgcct ccaggccggc cggtccaccg
     8881 cccaccacca gcacgtctcg cggggcggcg gccggcgtga tcggcgggtc ctcggtctcg
     8941 tggccgctcg ccggttcgcc gacgcagctg acgatcgggt tgcggttgtc gcgcacccgg
     9001 caccgctggt tgcacagcac gcacggccgg atccgcgcgc cgtgcccggc ccgccacaac
     9061 gcgaccaggt ccggctcggc gatctgcgcc cgggtcatct cgacgagctc ggccaccccg
     9121 tcgagggctt cttcggccat cgcggggtcg atcacgctgc cctgcaacac aaccgggcac
     9181 cgaccggcca ccgcggcccg gacctggcgg cacagttcga tgttgaaccc cggcggcgtg
     9241 tggaagtccg ggcgggtcgc cgacgtgccc atggccgagc cacgaacgac gaccaagtaa
     9301 tcgacggcat cggccacgga agccgccagc gaggcgccct ggtccggcgt gatcccggcc
     9361 cacggtgcca gctcgtcgca cgagagccgc aaccccagca cgccgtcgcc taccgacgcc
     9421 cgcaccacgg cgatcacctc gtgcagcagg gtggatttgt cgccatatcg atcatcgcgc
     9481 tggttggtca gccccgaaca gaactggcgc agcagggaga actggcccgc ttcgagctcc
     9541 acaccgtcca caccggacgc catggccagc ttggtcgccg cgccgaaccc ggcgatcaac
     9601 gcgtcgattt ccgcctgttc catggccatc ggcagctcac ggctcgccac gtccggcacc
     9661 cgcgagggcg cccacaacgc ctgctgcccg aacgccggcg agccttgcga cccgctgtgc
     9721 cccaacgacg ccagcacaag cgaaccctgg caggccgagg cgatggccgc ccagcccggt
     9781 ccgcactcgg cggccagcgg cgcccgctcg tacggccagt cggacgggtg cacgcttgcc
     9841 gtctcggtca cgatcacccc ggcaccaccg cgggcgcgtc gcgcgtagta ggcgacatgc
     9901 cggtcggaaa aggcgcggcc acgcccgagg ttcgtctcat gtggaccgaa gagcacccgg
     9961 ctgggggcct gccgaccggc cagcgtcacc ggatcggtga tcaccgcacc gccagcgggc
    10021 tggggtcgca cggtttcgcc ggcacgctgg gcatcccgag cgcgacccgc ttgcggtgcg
    10081 agtggtcctt gccctgagcc gggatggcga ggccggtcgt cggcgcgttt cccttgacgc
    10141 actcgggatc cggcccggcg agcggcaacc cggtgaagaa cttcgccgcc atgcacccgc
    10201 cctggcacgc gtcgtactcc ccgcagctcg cgcacgcgcc ggccgactgc ggctcgcgca
    10261 actgcgcgaa gagcggcgat tcccgccaca cgcggccgaa gccggcctca cgcacgttgc
    10321 ccgcgaggaa cgcgtcgtgg atcgcgaacg ggcacgcgta cacgtcaccg accgggtcga
    10381 tcaggcacac gacccggccc gcgccgcaca agttcaagcc tggcaacgaa gtcgaaccca
    10441 gggcgttgag gtggaagaac gagtcgccgg tgagcacgtt gtcgccgtgg cggaccagcc
    10501 agtcgtagat ctggcgctgc tggccctgtg tcgggtgcag gtcgtcccac acgtcggcgc
    10561 cgcgcccaga cggccgcagc cgggtgatgc gcagctgcgc gccgtagcgg tcggcgatgg
    10621 ccttgaactc gccgagctgc ggcacgttct gcctggtcat caccacggag accttgaaac
    10681 ccgcgaaccc ggcgtcggcg aggttcgcca tcgcggcgat cgccgtgtcg taggagccgg
    10741 gcccgcgcac ggcgtcgttg acctcgcggg tcgcgccgtc cagggagatc tgcacgtcga
    10801 cgtagtcggt cgcgacgagc tggcgcgccc gcagcggcgt gatgcgcagg ccgttggtgg
    10861 agaacttcac gccgacgtcg tgggtgacgg cgtagtgcat gagttcccag aagtccggcc
    10921 gcacggtcgg ctcgccgccg ccgatgttga cgtagaagac ctgcatccgc tgcagctcgt
    10981 cgatcaccgc cttgatctcg gcggtggtga gctccttggg gtcacgccgc ccggacgacg
    11041 acaggcagtg cgtgcacgag aggttgcagg cgtaggtcca ttcccaggtc aggcagatcg
    11101 gggcggacaa gcccttcttg aattggtcga ccagcttcat ccgcgcgtcc cttcgcaggt
    11161 caggcgggaa cgatcatgtc cgtctcggcg agcgtcgcga gggcccgcag gtagcggtcc
    11221 ttctcgggtg cggcgaggtg atcgaccgcg gccagcaccg tgggatgcgc ttcgaggtcg
    11281 cggacgagct cgaccagcgc gggagccttg agaaagctga gcttgcgcgt gccgaagtgg
    11341 taggcgagcg cgccgaaggg ttccggtcgc agcgccaccc tcgggttcag ccggtagggc
    11401 gccgccgggt cgaaccccgg agagtcagta gacaccgcac atgccgtcga tcgacacgtc
    11461 ctcgacgagg agttcctctt cgacctcgac cggttcctcg tccatggcat gctcctcagc
    11521 cagtgatcgg cgacacaccg ctagagtaat ggcactcggt gccgaatgga aggctcggtt
    11581 gtacggaaac gattccgcga cgcccgcgcg cggcggccgc aaaccggtga ccagccgcgc
    11641 cgaactggag cacgtcgcgt tcgacctgtt cgccgcgcag ggtttcgagg cgaccacggt
    11701 ggacgacatc gcgcgcgcgg ccggcatcgg gcggcgcacg ttcttccggt acttcccgtc
    11761 gaagaacgac atcccgtggg gcgccttcga cgagcaactg gaccaactgc gcaccgactt
    11821 gcgggcctac ccggtggacc agccgctgat ggacgtggtg cgggccgcgc tcgtcgactt
    11881 caaccgtgtg cccgcggccg aggtgccgtg gcaccggcgg cggatggcgt tgatcctgca
    11941 ggtgccggcg ttgcaggcgc actcgacgct gcggtacgcg aactggcggc aggtggtcgc
    12001 cgagttcgtg gccgaacggc tcgaccagcc tgtggacgcg ctcgggccgc agaccatcgc
    12061 gtacgcgacg ctcggcgtgg cgctcgcgtc gtacgagcag tggctgcgcg acgagtcgca
    12121 ggacctggcg gcgctgatgg cggcggcgat gaccgggttg gcggagagct tcaccggcgt
    12181 ccgcaccacc tgaccccggc ccgggcgtgt cccacgctca ggccgggtgt gtcccacatt
    12241 cgggtcgggt gtgtcccacc ttcaacgtgg gactcggggg accggaacgg gggacacgcg
    12301 gggccggaac gggggacacg ccccggggtg gtcagttggt caggaacatc acgatgatga
    12361 tcagcagggc cgggagcagg gcccaccacg tcgtcgtcac ggcggcgacc accacggccg
    12421 cgacgatcat gatcgcgccg agggcgagca cgatggggcg gctcagccgg gccgggctgt
    12481 tgtcgatcag cggggactgc tccggcgcgg tggccggttc gagctcctcg ggatcccact
    12541 tcgggtgcgg gaccggcagg tcgtcgaaaa ccgccagcag atcggcgcga gtgcgggcgg
    12601 ccgtcaccgc ggcgcagcgt tgctcgtact cggccacgtc cagccggtcc gccgtgaggt
    12661 gctcggccag ggcatgcaat gcgctttctc gctccgcgct gccgacccgg atgttggcgc
    12721 tgcctgccat ggtcacgatc ctacggaatg cactcgcgcc gtcacagccc gaacaacctt
    12781 cgcgcgttgc cgccggcgat cttgcgccgg tcgctctcgc tgatcggtgc gcggtcgagc
    12841 cacgtggtcg cgagttccgt gtcggcgtac gggtagtcga cggagaacag gacccggtcg
    12901 ccgccgacgg tcagcagcgc ggtgatcaac gcctggtcgc tgaagttgcc gctcgtggtg
    12961 acgtggatgt tgtcggcgaa gtactccggc agggttttct cgatgcgctt gtcgaacggg
    13021 ttgaggtcgt agcagtactg cgtgcgccag agctgcgccg gcaggccctc gcccaggtgg
    13081 ccgagcacca actgcacgcc ggggaaccgg tcgaacagac cgctgaagat caaccgcagc
    13141 gtgtgtgtgg ccgtctcgac gccgaagccc caggtcgcgc cgaccagctg cgggtggccg
    13201 gcgatcgcgt gccggtcggt cggcggcgcc gggcgcgggt gcaggtagac cggcacgccc
    13261 aggtcggcga ccgcctccca gaagccggtg aaccgctcgt cgtcgaggta ggcgtcgccg
    13321 ccgtcaccgg tggccgtgta gccgttgacc atggcgccga cgaacccgag ttcgcccacg
    13381 cagcggcgca actcctcggc ggccgcggcc gggtcctgca acgccaccga ggcgaagccg
    13441 cggtagcggt cggggtgctt gcgcacctgc tcggcgagca ggtcgttggc gtcgcgggcc
    13501 atcgcgatcg cgtcggccgt gccctcgacg agctggatgc cgggggcggt cagcgacagc
    13561 accgcgcact cgatgccgaa cttgtccatt gtggacaacc gctggtcgtc gaagtcgccg
    13621 agcaggccca ggatcgggcg gtagtactcg ggcatgacgc cgccgccgtc ggagaggcgc
    13681 tggagcttgt cggggtcggc gaggacgtcg gcggcggccc gcggatcgag gaagtgctcc
    13741 tcgagcgcga tcttggtcag cggcatgcca ccagtgaacc gcgttactca gaactcgtcg
    13801 aacaggttcg gctccagggc gccctcgatc accaggatcc ggcgcttggt ctcgacgcca
    13861 ccggtcgcgg agaacccgcc cagcttgccg cccgcggcca gcacccggtg gcacggcacg
    13921 accagcgggt acgggttgtt gcccatcgcc tgaccgaccg cccgcgcggc cagcggatca
    13981 ccgagctgct tggcgatctc gccgtaggtg atcgtcttgc cgggcggcac ggtgcgggcg
    14041 acctcgtaga cggagcggtc gaactcggtc acctcggacc agtcgacctc gacgtcgctc
    14101 aggtcgctct tctctccacc cagcagcgcc gcgatccgcg tgatggcgtc ggccagcggc
    14161 tgcggcggct tgtcccggcg gacgtcgggc cagcgccgac gcatgcgcac ctcggcgcgc
    14221 aggtcggtcg gctcgggcag gccgaccccc accacgccgt tcgcacccca ggcgatggag
    14281 cagcgcccga tcggggtgtc gaacaatgtg aagctgacct cggtcatggt gaagagtgtg
    14341 caccggggca ccgacagaac atgagatgcc cgacaagcgc ttggtttacc gatagtagga
    14401 cgacaaacta tccgcattcg gtcgtcgtgg agggaactgc acatgggtga ggtcagcagg
    14461 gtcggtgttg tcggctcggg cctgatggga tcggggatcg ccgaggtctg cgcgcgagcc
    14521 gggctcgacg tgctggtcaa cgaggtgaac caggacgcat ccgacgcggg ccgggcccgc
    14581 atcgagaagt cgctggcccg cggcgtgcgc agcggcaaac tggccgagga cgaccgggac
    14641 gccgcgctgg cccgcctttc gttcaccacc gacctcggtg acttcgccga ccgcgacatc
    14701 gtgatcgagg ccgtcgccga gcacgaggcc acgaaggtcg acgtgttccg caccctggac
    14761 aaggtggtgc gccgcgagga cgccttgttc gcgtcgaaca cgtcgtccat cccgatcatg
    14821 aagctgggca tggccacgag ccggcccgcg caggtcatcg gcgtgcactt cttcaacccc
    14881 gtgccggtgc tgcccctggt ggagctggtg ccgtcgctgc tgaccagcga cgagacccgc
    14941 gcggccgcgg aggaattcgt caccgggccg ctgggcaagc aggtcatcca gtcccaggac
    15001 cgggccggct tcgtggtcaa cgcgctgctc atcccgtacc tgctgtcggc gatccggatg
    15061 atggagtcgg gcttcgcctc ggccgaggac atcgaccagg gcatcgtgct gggcctggcg
    15121 cacccgatgg gcccgctgcg cctgaccgac ctgatcggcc tcgacacgac caaggcgatc
    15181 gccgagtcga tgtacgcgga gttcaaggag ccgctgtact ccccgccgcc gctgctgctg
    15241 cgcatggtgg acgcgggtct gctcggcaag aagtctggcc gggggttcta cgactacgag
    15301 gtggcgaagt gacgggtctg taccagctcg gcgaggagca cgaggagctg cgcgcggcca
    15361 tccgggcgtt ggtggacaag gagatcgcgc cgcacgcggc cgcggtcgac gaggacgccc
    15421 ggttccccga ggaggcgcgc aaggcgctgg tggccgcgga catgcacgcg gtgcacatcc
    15481 cggagttcta cggcggccag ggtgccgatg ccgtcgccgc gtgcatcgtg atcgaggagg
    15541 tcgcgcgggc gtgcgcgtcc tcgtcgctga tccccgcggt gaacaagctg gggacggtgc
    15601 cgttgctgct gtccgcgtcc gaggagctga agtcccaggt gctgccgggc atcgcgaagg
    15661 gcgagatggc ctcgtacgcg ttgtccgagc gcgaagccgg gtcggacacg gcgtcgatgc
    15721 gcacgcgggc gcgtctcgac ggcgaccact gggtcctcaa cggcacgaag gcgtggatca
    15781 ccaatgccgg tgaatccacg tggtacaccg tgatggccgt gacggacccg aatgccgcaa
    15841 aaccggcaaa cgggatttcc gctttcgtcg tgcacaagga cgacccgggt ttttccgtcg
    15901 gaccgaagga acgcaaactc ggcatcaagg gctcgccgac ccgcgagatc catttcgagg
    15961 actgcacgat tcccgccgac cgcatcgtcg gcgaaccggg caccgggctg aagaccgcgc
    16021 tggcgacgct ggaccacacg cgtccgacca tcggcgccca ggccgtgggc atcgcgcagg
    16081 gtgcgctcga ggcgtcgatc gcgtacgtca aggaacgcaa gcagttcggc aaggcgatcg
    16141 ccgagttcca gggcttgcag ttcatgctcg cggacatggg gatgaaggtc gaggcggctc
    16201 gccacctggt ctacgccgcg gcggccaagg cggagcgtgg cgacccgggc gccgggttca
    16261 cggccagcgc ggcgaagacg ttcgcgtcgg acaccgccat gcaggtgacc acggatgccg
    16321 tgcagctctt cggcggtgcc ggatacacgc gtgacttccc ggtggaacgc atgatgcggg
    16381 acgcgaagat cactcaaatc tacgaaggca cgaaccagat ccagcgggta gtgatggcgc
    16441 ggcagctact caaaggatga gcgaagcgat cgaatcgaaa tacttgatcg atactgaacc
    16501 ctggtaaccg ggcacgaccc cgcttacgtt tccctgcgaa cgtcggggac cttttggctc
    16561 gcgcatcgtg cggaggcagg gaaatgggtc agtacagaaa acgctgggtg gcgggggcga
    16621 cggcgacgtt gctggccgcg atgacggtcg tgctcgggca cgacgcctcc gcggccccgt
    16681 ccgaccggcg agtgctcgcg ggcagcgtcc cgccgtgggc ggcggcggcc aaccgggcga
    16741 gcaacgcgcc ggacaccgac agcgtcggct tccgggtcta cctgggctgg cagaaccagt
    16801 cgggcgccga ggcactcgcc gcgcgggtgt cgacgccggg cagcgcggac tacggcaagt
    16861 tcgtctcgcc cgcccagttc cgggcgcagt tcgcgccgtc gccgtcggac gccagcgcgg
    16921 tgcagcagtg gctgcgcaag tccggcttcg acatcagcac cacgccgggc aacaaccggt
    16981 acgtgcaggc cgagggcacc gtggcccagg cgaaggcggc cttcggcgcc ggcttcggtg
    17041 agtaccgggt ggccggcaag acgctgcgcg cgccggacgg tgccctgtcc gtgcccgccg
    17101 acctgcccgc ctcggtcacc tcggtggtcg gcctcgacga gtcggccgcg ctgaccgtgc
    17161 cgagcgcgcc gccgtcgccg gtgttcgtca acgccgcacc ctgctcggcc tactggaacg
    17221 agaagaacac ggcgaccacg ctcaccccga acggcaccaa ggcacccgac gcctacggcc
    17281 accccaaccc gtgggctccg tgcggttaca cgcccgcgca actgcgcagc gcctacggca
    17341 tgaccagctc ggccgccaac ggcagcggcc agaccgtggc catcatcgac gcctacgcct
    17401 cgccgaccat cgtgcaggac gtcaacacct actcgtcgct gcacgggctg cccgcgctga
    17461 acggctcgaa cttcagccag gtcaccccgc ccggcgtgtt caagcgcccg cagaacagcc
    17521 accaggaccc gcagggctgg tacggcgagg agaccctcga cgtcgaggcc gtgcacggca
    17581 tggcgcccgg cgcgaagatc gtctacgtcg gctcgccgaa caactaccag gacctcgacg
    17641 cggccatgaa ccacgtcgtg gaccagcacc tggcgtcgat cgtgaccaac tcctacggct
    17701 tctccaccga gctcctgccg cgcggctacg tcaagccgct caacgacacg ctcatcgagg
    17761 ccgcggccac gggtgtcggc gtctacttct cctcgggtga caacggcgac gagaccgcgg
    17821 gcgacccggc caacgccgcg tcggccaccc cggactggcc ggccgtcagc ccgtgggtca
    17881 ccgcggtcgg cggcaccgcc atcgccatcg gcgccagtgg caactacctg ttcgaggccg
    17941 gctgggaaac cggccgctcg aacctggtga acggcgcctg gtcgccgacc ccgcccggca
    18001 actacaccag tggttccggc ggtggcacga gccgcctctt cacccagccc gcgtaccagg
    18061 cgggtgtggt gcccaacgcg atcgcgacgg cgaacggcgc ccggccgcag ccgatgcgcg
    18121 tcgtgccgga cgtcgcctcg atcgccgacc cgaacaccgg gatgctcgtc ggccagacgc
    18181 agacgttccc ggacgggcac gcggcgtaca gcgagtaccg catcggtggc accagcctgg
    18241 cctcgccggt gttcgcgggc atggtcgcgg tcgcgcagca gcaggcgggc cgcacgttcg
    18301 gcttcgccaa cccgctgttc taccgcaacg cgggcacgtc ggcgttccac gacatcgtgc
    18361 cgccggcggc accggtcgcc gtggcgcgca acgacttcgc gaacacggtc gactcgtccg
    18421 agggctacgt cgtcagcctg cggacgctcg acttcgacgc gaacctcacc atccacaccc
    18481 ggcccgggta cgacgacgtg accgggatcg gctcgcccaa cggagcggcc tggctgagcg
    18541 ccttggcggc cggctgagat acatcggcac aacggcacaa cggcacaacg gcaacggccc
    18601 cacacgcccg cctggcgtgt ggggccgttg ccgtgacggc tcgctgacgt cttgggactg
    18661 gctgaagctg gtcttaccgg ccgccgccga cgccgaacgg tgggactacc tcgtgaactc
    18721 cacgacccgc cgcgaagcgg agcaggcgcc tgcctagtcc caccagaggt cggggtcggc
    18781 cgccgcgtca tcctcgtcca cggactccgg gtgcggcttg tacaggtgct cgtggctgac
    18841 ctcgtgcacg gccacggcgt cgaaggcggc cagcatctcc tcggtgggca ggtcctcgtg
    18901 caccgccagc tgcgtggccg cctccatccg cagcaggtgc agcgcgagcg actcggcgat
    18961 cgtgcccggc gggatccacg ccggctgggt caggcggtag ccgacgatca ccatcgtgat
    19021 caggaactgg cgcgcgaagt gggcggtgta ccggtcgcgg tagcgctcgg gcagcagcgg
    19081 gagcacggcc agttcctcgt gctcgtcgac ggtcgtgtcg ccgtcctcga gggccgcgat
    19141 gtcgccgaac aactcgccga ccaggatctc cgccgacgtg atcagcgcgc ccgccagcag
    19201 ctgcgcgtgc tcctcggcga ggtcgtcgaa ctcgaacgca cgcagcgcgc cgcccgcgtc
    19261 ggccagctgg tcgaggtgtt cttcgaggtc ggcctcggtc agctcgtcct cttcctcgtc
    19321 ctgcggcggc tcgtcggaca tggccgcctc cagggcggcg tcgctcgcgg tgacctcgca
    19381 gcgctgcacc tgccagcggg ccagcagttc ggtctgcgcg agcagctcgt cgagcacgtg
    19441 cttcgcgcct tcctcggcga acgcgagggc gggcgcctcc accagccact cgatgtgggc
    19501 gccggtggtg gtgacgtcga tgcggtggtc gaggggttcg atctccaccc cgtccgggcc
    19561 ctcgatgccc gcgagcatgt cgagctgctc gtcgagcagg gcggcgacgc cgtggcgttg
    19621 caacgggtcg agctccgcgg tgtcgggcgg cggaacgaga agggcggtca gctggtactc
    19681 cactcgcagg agtttcgcat cgcccgctcg cgcgccggtc accagggtgc gggctcgaaa
    19741 tccttcagga agcagccgta caggtcctcg cccgcctcgc cgcgcacgat cgggtcgtac
    19801 acccgcgcgg cgccgtccac gaggtccagc ggcgcgtgga aaccctcgtc ggccagccgc
    19861 atcttcgtcg ggtgcggccg ttcgtcggtg atccagccgg tgtccacggc ggtcatcagg
    19921 atgccgtcgt cgaccaggtc gaacgcgctc gtgcgggtca gcatgttgag cgcggccttg
    19981 gccatgttcg tgtgcgggtg cccggcgccc ttgtaagccc ggctgaactg gccttccatg
    20041 gccgagacgt tcaccacgta cttgcgccgc gccgggctcg ccagcatggc cgcgcgcagc
    20101 cgcgagacca ggatgaacgg cgcggtctgg ttgcacagct gcacttccag cagttcggtc
    20161 gggtcgacct cgtgcacccg gtccacccag ctgttcgtcg ccgcgagatc gggcacgaga
    20221 ccgcccgcgt cgatggccgt gcccgcggcg acgcggtccg gcgacgcgga tttggccacc
    20281 agagcgagtt cggtgacggg caccgacgcc aacgcgatcg ggtgctcctc ggcggttcgg
    20341 ccgaacgtga tcatctccgg cagctcaccc gcgggcaatg gtccggactc ggcggcgatc
    20401 agctccgcgt aggcgccgga gctgcgccgg acggtctgcg cggcgttgtt gatcaggatg
    20461 tccagtgggc cctgctcggc cacggagtcg gcgagcgcga cgacctgggc ggggtcgcgc
    20521 aggtcgatgc cgaccacgcg cagccggtgc agccactcgg cgctgtcgtc catcgcgctg
    20581 aagcggcgga tggcgtcctt gggaaaccgg gtggtgatcg tggtgtgcgc gccgtcgcgc
    20641 agcagccgca acgcgatgta catgccgatc ttggcgcggc cgccggtgag cagcgcgcgc
    20701 cggcccgtga ggtccacacg ggcgtcgcgg cggtcgcggt tgcgggcggc gcacggcggg
    20761 cacagctggt ggtagaacgc gtcgacctgg gtgtagcgct tcttgcaggt gtagcaagag
    20821 cgcgcgcgct ggtactctcc cgcgctcgcc ccagggaccg tggacaccag cgggatgccc
    20881 ttggtctcgt cgtcgatccg atcgggtgaa cccgttgcgg tgcgggcaat cacctcgcgg
    20941 tcggcttccg tgatggccgc gcgcttggcc gccttcttgc gctggcgcgc gaccttgtac
    21001 atccgcgagg tggcccggcg gaccacgacg gcgtcggggt ggtccgtggg cagcagctcg
    21061 acttcggcca gcaccgcgag gcactcacgc aagcggtcgg ggtcgacacc ggtcatgagt
    21121 gcagcctatg aggtcgcgta gcctggtggt cgttgcggtg agtacacgtg gttgggctga
    21181 ggtgatcctg gtggcgaccg gcaaggtgct gcggttcgac gacacgcggg ggtacgggtt
    21241 catcgccccg gacgacggcg gcgatgacgt gttcatgcac gccaacgacc tgttggacga
    21301 gaagtacctg ttccgcgcgg gtgtccgggt cgagttcgac atctccgacg gggaccgcgg
    21361 gctgaaggcg tcggaggtcc gggtggtcga ccgcgatacg gtgacccgag acgtccgtga
    21421 cgacacgctg tgtga
//
