GCF_004346925.1 - 7 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP031167.1 (Stenotrophomonas sp. ASS1)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	RiPP-like	1,138,862	1,149,707
Region 2	RiPP-like	1,421,745	1,432,620
Region 3	lanthipeptide-class-ii	1,653,959	1,676,856
Region 4	lassopeptide	2,298,572	2,321,126
Region 5	NRP-metallophore,NRPS	2,535,197	2,583,564	High	2,3-dihydroxybenzoylserine	NRPS:Type I
Region 6	terpene-precursor	3,194,290	3,215,168
Region 7	arylpolyene	4,378,897	4,422,499	Low	APE Vf	other:other

NZ_CP031167.1 - Region 1 - RiPP-like

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Location: 1,138,862 - 1,149,707 nt. (total: 10,846 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.37	PKS	curacin A	Moorea producens 19L
BGC0001484.3	0.33	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0002000.4	0.32	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000527.5	0.31	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002571.2	0.31	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0001789.3	0.31	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.31	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.31	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000537.3	0.31	ribosomal	nisin U	Streptococcus uberis
BGC0002047.2	0.30	PKS	brasiliquinone A, brasiliquinone B	Nocardia brasiliensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.37	PKS	curacin A	Moorea producens 19L
BGC0001484.3	0.33	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0002000.4	0.32	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000527.5	0.31	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002571.2	0.31	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0001789.3	0.31	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.31	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.31	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000537.3	0.31	ribosomal	nisin U	Streptococcus uberis
BGC0002047.2	0.30	PKS	brasiliquinone A, brasiliquinone B	Nocardia brasiliensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.77 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.47 out of 23.79

Confidence: medium

Additional weak hits (1):

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.64 out of 32.93

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP031167.1 - Region 2 - RiPP-like

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Location: 1,421,745 - 1,432,620 nt. (total: 10,876 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001962.3	0.27	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0000188.5	0.23	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001650.4	0.23	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0000452.5	0.21	NRPS	tyrocidine	Brevibacillus brevis NBRC 100599
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus
BGC0001283.3	0.18	PKS	Bisenarsan	Streptomyces lividans 1326

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001962.3	0.27	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0000188.5	0.23	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001650.4	0.23	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0000452.5	0.21	NRPS	tyrocidine	Brevibacillus brevis NBRC 100599
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus
BGC0001283.3	0.18	PKS	Bisenarsan	Streptomyces lividans 1326

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.19 out of 19.26

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.64 out of 26.85

Confidence: medium

Additional weak hits (4):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.25 out of 27.78

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.36 out of 29.38

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP031167.1 - Region 3 - lanthipeptide-class-ii

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Location: 1,653,959 - 1,676,856 nt. (total: 22,898 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

MG068_RS07820: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.50	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002346.3	0.45	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.45	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002703.2	0.44	ribosomal	archalan α	Halorussus salinus
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002704.2	0.41	ribosomal	archalan β	Halorussus salinus
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002698.2	0.39	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001576.3	0.39	ribosomal	divamide A	Prochloron didemni

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.50	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002346.3	0.45	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.45	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002703.2	0.44	ribosomal	archalan α	Halorussus salinus
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002704.2	0.41	ribosomal	archalan β	Halorussus salinus
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002698.2	0.39	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001576.3	0.39	ribosomal	divamide A	Prochloron didemni

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for MG068_RS07820

MG068_RS07815

Leader:

MDLNDDVVSGWLNGSDDVDGCANPAGSLFIYGAAATEAALTGASDTMITGG

Core:

TNECTTTVSVINHCLCC

Core with dehydrations:

DhbNECysDhbDhbDhbVDhaVINHCysLCysCys

antiSMASH-DB 4.0 matches for MG068_RS07815

100.0%

NZ_CP011305.1and 19 others:

NZ_CP031058.1 Lanthipeptides: DUW70_RS15590_lanthipeptideNZ_CP031167.1 Lanthipeptides: MG068_RS07815_lanthipeptideNZ_CP040436.1 Lanthipeptides: FEO91_RS08085_lanthipeptideNZ_CP046588.1 Lanthipeptides: GOC52_RS08100_lanthipeptideNZ_CP060259.1 Lanthipeptides: H7F32_RS08235_lanthipeptideNZ_CP071784.1 Lanthipeptides: J3U96_RS13765_lanthipeptideNZ_CP077679.1 Lanthipeptides: KS456_RS07940_lanthipeptideNZ_CP096975.1 Lanthipeptides: M1L61_RS08375_lanthipeptideNZ_CP104071.1 Lanthipeptides: K7565_RS07800_lanthipeptideNZ_CP104287.1 Lanthipeptides: K7567_RS07875_lanthipeptideNZ_CP104324.1 Lanthipeptides: K7569_RS08320_lanthipeptideNZ_JACLBK010000002.1 Lanthipeptides: ID844_RS05155_lanthipeptideNZ_JAEDVP010000027.1 Lanthipeptides: JAK38_RS15550_lanthipeptideNZ_JAEDVW010000007.1 Lanthipeptides: JAK53_RS03160_lanthipeptideNZ_JAEDWD010000009.1 Lanthipeptides: JAK45_RS02000_lanthipeptideNZ_JAFLWT010000006.1 Lanthipeptides: J1F00_RS06425_lanthipeptideNZ_JAQQGI010000014.1 Lanthipeptides: PQS91_RS16925_lanthipeptideNZ_KB907502.1 Lanthipeptides: G370_RS0114170_lanthipeptideNZ_RHPZ01000014.1 Lanthipeptides: EGJ03_RS16365_lanthipeptide

Lanthipeptides: YH67_RS07965_lanthipeptide

1

TNECTTTVSVINHCLCC

17

TNECTTTVSVINHCLCC

1

TNECTTTVSVINHCLCC

17

24.1%

NZ_CP089924.1:

Lanthipeptides: LVB77_RS02735_lanthipeptide

4

CTTTVSVINHC

14

CT+ SV NHC

8

CTSGSSVSNHC

18

22.9%

NZ_CP011010.1and 27 others:

NZ_CP015612.1 Lanthipeptides: A7326_RS08120_lanthipeptideNZ_CP027562.1 Lanthipeptides: C6Y55_RS08375_lanthipeptideNZ_CP028358.1 Lanthipeptides: C9J74_RS08630_lanthipeptideNZ_CP040435.1 Lanthipeptides: FEO92_RS08800_lanthipeptideNZ_CP040438.1 Lanthipeptides: FEO89_RS08350_lanthipeptideNZ_CP040439.1 Lanthipeptides: FEO88_RS08780_lanthipeptideNZ_CP043578.1 Lanthipeptides: F0Q13_RS08255_lanthipeptideNZ_CP047310.1 Lanthipeptides: GS396_RS07720_lanthipeptideNZ_CP056088.1 Lanthipeptides: HUZ96_RS08220_lanthipeptideNZ_CP067993.1 Lanthipeptides: JJL50_RS07700_lanthipeptideNZ_CP090418.1 Lanthipeptides: LYZ64_RS08310_lanthipeptideNZ_CP104169.1 Lanthipeptides: NDR96_RS06465_lanthipeptideNZ_CP109812.1 Lanthipeptides: FQS62_RS08595_lanthipeptideNZ_DS999412.1 Lanthipeptides: SSKA14_RS15505_lanthipeptideNZ_JABDRV010000001.1 Lanthipeptides: HRL60_RS01085_lanthipeptideNZ_JAEDUZ010000008.1 Lanthipeptides: JAK52_RS03215_lanthipeptideNZ_JAEDVS010000025.1 Lanthipeptides: JAK50_RS15965_lanthipeptideNZ_JAEDWK010000010.1 Lanthipeptides: JAK51_RS04155_lanthipeptideNZ_JAJNMF010000022.1 Lanthipeptides: LQ423_RS11465_lanthipeptideNZ_JVHP01000063.1 Lanthipeptides: ADI44_RS21105_lanthipeptideNZ_JVPM01000038.1 Lanthipeptides: ADH50_RS19075_lanthipeptideNZ_JVUI01000059.1 Lanthipeptides: ADG58_RS15825_lanthipeptideNZ_LR134301.1 Lanthipeptides: EL200_RS07985_lanthipeptideNZ_OBEE01000002.1 Lanthipeptides: CRO53_RS02030_lanthipeptideNZ_OU943334.1 Lanthipeptides: LNN95_RS09005_lanthipeptideNZ_RHQE01000015.1 Lanthipeptides: EGJ89_RS13150_lanthipeptideNZ_SOCO01000001.1 Lanthipeptides: N440_RS05690_lanthipeptide

Lanthipeptides: VN11_RS08230_lanthipeptide

8

VSVINHCLCC

17

+SV+NHCLCC

26

MSVVNHCLCC

35

with 1 more sequence matches

13.8%

NZ_CP007597.1:

Lanthipeptides: DX03_RS09815_lanthipeptide

8

VSVINHCLCC

17

+SVINHC+CC

49

MSVINHCMCC

58

allorf_1655407_1655520

Leader:

MALTVLPWMALPALFTVSVLAA

Core:

NSTPTCCAASAQPMI

Core with dehydrations:

NDhaDhbPDhbCysCysAADhaAQPMI

antiSMASH-DB 4.0 matches for allorf_1655407_1655520

100.0%

NZ_CP031167.1:

Lanthipeptides: allorf_1655407_1655520_lanthipeptide

1

NSTPTCCAASAQPMI

15

NSTPTCCAASAQPMI

1

NSTPTCCAASAQPMI

15

21.7%

NZ_AP017635.1:

Lanthipeptides: allorf_1003827_1003931_lanthipeptide

7

CAASAQPMI

15

C ASA P++

13

CVASADPLV

21

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 27.17 out of 32.74

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

Additional weak hits (8):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.98 out of 39.62

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.46 out of 32.74

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.90 out of 30.22

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.79 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.47 out of 29.65

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.77 out of 23.43

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.18 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.00 out of 24.78

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

MG068_RS07815 - Class II

Cleavage pHMM score: -4.40

RODEO score: 0

Monoisotopic mass: 1749.8 Da

Molecular weight: 1751.0 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 1769.0 Da N = 1

allorf_1655407_1655520 - Class II

Cleavage pHMM score: -4.30

RODEO score: 0

Monoisotopic mass: 1421.6 Da

Molecular weight: 1422.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1440.7 Da N = 1; 1458.7 Da N = 2

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
FuR variant 2	Ferric Uptake Regulator

NZ_CP031167.1 - Region 4 - lassopeptide

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Location: 2,298,572 - 2,321,126 nt. (total: 22,555 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

MG068_RS10865: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

MG068_RS10895: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000577.3	0.55	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0001786.3	0.53	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000580.3	0.51	ribosomal	xanthomonin II, xanthomonin I	Xanthomonas gardneri ATCC 19865
BGC0000573.3	0.50	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0001779.5	0.46	ribosomal	siamycin	Streptomyces nodosus
BGC0001889.2	0.44	ribosomal	fusilassin	Thermobifida fusca YX
BGC0000623.3	0.43	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0002825.2	0.43	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0002015.4	0.42	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001645.3	0.42	ribosomal	lagmysin	Streptomyces sp. NRRL S-118

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000577.3	0.55	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0001786.3	0.53	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000580.3	0.51	ribosomal	xanthomonin II, xanthomonin I	Xanthomonas gardneri ATCC 19865
BGC0000573.3	0.50	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0001779.5	0.46	ribosomal	siamycin	Streptomyces nodosus
BGC0001889.2	0.44	ribosomal	fusilassin	Thermobifida fusca YX
BGC0000623.3	0.43	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0002825.2	0.43	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0002015.4	0.42	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001645.3	0.42	ribosomal	lagmysin	Streptomyces sp. NRRL S-118

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for MG068_RS21080

MG068_RS21080

Leader:

MNELIELGVVAGQQNPEDQGMGGELIELGSVVSETK

Full core:

AHNHGTLWDGVFPTTSP

Putative macrolactam:

AHNHGTLWD

antiSMASH-DB 4.0 matches for MG068_RS21080

100.0%

NC_011071.1and 41 others:

NZ_AP021867.1 Lassopeptides: GJU96_RS20605_lassopeptideNZ_CP011010.1 Lassopeptides: VN11_RS22530_lassopeptideNZ_CP011305.1 Lassopeptides: YH67_RS21020_lassopeptideNZ_CP015612.1 Lassopeptides: A7326_RS21600_lassopeptideNZ_CP027562.1 Lassopeptides: C6Y55_RS23185_lassopeptideNZ_CP029773.1 Lassopeptides: DM611_RS22725_lassopeptideNZ_CP031058.1 Lassopeptides: DUW70_RS11975_lassopeptideNZ_CP031167.1 Lassopeptides: MG068_RS21080_lassopeptideNZ_CP037858.1 Lassopeptides: EZ304_RS20935_lassopeptideNZ_CP040432.1 Lassopeptides: FEO95_RS22840_lassopeptideNZ_CP040438.1 Lassopeptides: FEO89_RS21625_lassopeptideNZ_CP040439.1 Lassopeptides: FEO88_RS23220_lassopeptideNZ_CP043578.1 Lassopeptides: F0Q13_RS11365_lassopeptideNZ_CP046588.1 Lassopeptides: GOC52_RS10870_lassopeptideNZ_CP047310.1 Lassopeptides: GS396_RS20180_lassopeptideNZ_CP060259.1 Lassopeptides: H7F32_RS11180_lassopeptideNZ_CP067993.1 Lassopeptides: JJL50_RS10815_lassopeptideNZ_CP077679.1 Lassopeptides: KS456_RS10490_lassopeptideNZ_CP080573.1 Lassopeptides: K1516_RS11235_lassopeptideNZ_CP090418.1 Lassopeptides: LYZ64_RS11980_lassopeptideNZ_CP096975.1 Lassopeptides: M1L61_RS12085_lassopeptideNZ_CP104169.1 Lassopeptides: NDR96_RS09175_lassopeptideNZ_CP106759.1 Lassopeptides: N8888_RS09665_lassopeptideNZ_CP120400.1 Lassopeptides: PVE91_RS09960_lassopeptideNZ_JABDRV010000002.1 Lassopeptides: HRL60_RS08285_lassopeptideNZ_JADCQA010000003.1 Lassopeptides: IMX13_RS03920_lassopeptideNZ_JAEDVS010000016.1 Lassopeptides: JAK50_RS09365_lassopeptideNZ_JAEDVW010000040.1 Lassopeptides: JAK53_RS10000_lassopeptideNZ_JAEDWA010000005.1 Lassopeptides: JAK63_RS01795_lassopeptideNZ_JAEDWK010000019.1 Lassopeptides: JAK51_RS11395_lassopeptideNZ_JAEDWU010000002.1 Lassopeptides: JAK37_RS06750_lassopeptideNZ_JAKJQX010000001.1 Lassopeptides: MT466_RS00850_lassopeptideNZ_JAQQGI010000001.1 Lassopeptides: PQS91_RS01155_lassopeptideNZ_JVHP01000037.1 Lassopeptides: ADI44_RS25485_lassopeptideNZ_JVPM01000019.1 Lassopeptides: ADH50_RS20965_lassopeptideNZ_JVUI01000038.1 Lassopeptides: ADG58_RS23065_lassopeptideNZ_OBEE01000001.1 Lassopeptides: CRO53_RS21815_lassopeptideNZ_OU943334.1 Lassopeptides: LNN95_RS11880_lassopeptideNZ_RHPZ01000001.1 Lassopeptides: EGJ03_RS21120_lassopeptideNZ_RHQE01000001.1 Lassopeptides: EGJ89_RS22430_lassopeptideNZ_SOCO01000001.1 Lassopeptides: N440_RS20340_lassopeptide

Lassopeptides: SMAL_RS21110_lassopeptide

1

AHNHGTLWDGVFPTTSP

17

AHNHGTLWDGVFPTTSP

1

AHNHGTLWDGVFPTTSP

17

88.2%

NZ_CP058243.1:

Lassopeptides: AD14011_RS10295_lassopeptide

1

AHNHGTLWDGVFPTTSP

17

AH HGTLWDG+FPTTSP

1

AHGHGTLWDGIFPTTSP

17

61.1%

NZ_CP049017.1:

Lassopeptides: G4Q83_RS07645_lassopeptide

1

AHNHGTLWDGVF-PTTSP

17

AHNHGT + F TTSP

1

AHNHGTFLEASFIATTSP

18

with 5 more sequence matches

29.2%

NZ_CP033362.1:

Lassopeptides: EB232_RS01185_lassopeptide

6

TLWDGVFPTTSP

17

T DGVFP +P

6

TALDGVFPIHTP

17

22.2%

NZ_CP044492.1and 1 other:

NZ_CP066294.2 Lanthipeptides: IGS65_RS08185_lanthipeptide

Lanthipeptides: FSA31_RS00605_lanthipeptide

8

WDGVFPTTS

16

W GV PT S

4

WQGVVPTVS

12

22.2%

NZ_CP049223.1and 4 others:

NZ_CP049225.1 Lanthipeptides: G6Z85_RS06555_lanthipeptideNZ_CP049228.1 Lanthipeptides: G6Z83_RS06555_lanthipeptideNZ_JAKWZB010000003.1 Lanthipeptides: MMO78_RS01405_lanthipeptideNZ_PNGO01000002.1 Lanthipeptides: CJ224_RS02730_lanthipeptide

Lanthipeptides: G6Z86_RS06445_lanthipeptide

8

WDGVFPTTS

16

W GV PT S

4

WQGVLPTVS

12

22.2%

NZ_FNUH01000004.1and 1 other:

NZ_FOGM01000011.1 Lanthipeptides: BMX20_RS07220_lanthipeptide

Lanthipeptides: BLU50_RS05815_lanthipeptide

7

LWDGVFPTTS

16

W GV PT S

3

FWQGVVPTVS

12

13.9%

NZ_CP015883.1and 1 other:

NZ_JAASJP010000011.1 Lanthipeptides: allorf_43013_43183_lanthipeptide

Lanthipeptides: allorf_0205278_0205448_lanthipeptide

3

NHGTLWDGVF

12

N+G +W+ VF

16

NNGCMWNTVF

25

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.73 out of 23.79

Confidence: medium

Additional weak hits (9):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.37 out of 36.54

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.39 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 23.30 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.21 out of 33.14

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 26.30 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.01 out of 33.14

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.34 out of 35.63

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.10 out of 30.11

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

MG068_RS21080 - Class II: Cleavage pHMM score: 10.60; RODEO score: 22; Monoisotopic mass: 1817.8 Da; Molecular weight: 1818.9 Da; Number of bridges: 0

RRE predictions

: RRE type: Lasso_Fused_RRE; Location: 6 - 77; Score: 47.5

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_CP031167.1 - Region 5 - NRP-metallophore,NRPS

Download region GenBank file

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Location: 2,535,197 - 2,583,564 nt. (total: 48,368 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

MG068_RS11960: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MG068_RS12000: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

MG068_RS12020: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

Hydrolases: hydrolase

MG068_RS11975: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: isomerase

MG068_RS11985: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

MG068_RS11965: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MG068_RS11980: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002689.2	1.45	NRPS	2,3-dihydroxybenzoylserine	Stenotrophomonas maltophilia K279a
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000343.5	0.63	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002689.2	0.90	NRPS	2,3-dihydroxybenzoylserine	Stenotrophomonas maltophilia K279a
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000343.5	0.65	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.12 out of 23.79

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.67 out of 20.01

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.02 out of 25.31

Confidence: medium

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 33.52 out of 39.62

Confidence: medium

Additional weak hits (24):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.89 out of 21.55

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.81 out of 26.06

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.98 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.08 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.16 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.59 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 26.18 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.71 out of 31.73

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 23.91 out of 30.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.38 out of 27.78

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.32 out of 26.12

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.43 out of 32.93

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 22.33 out of 31.73

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.47 out of 39.62

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.58 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.91 out of 29.38

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.59 out of 24.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.56 out of 28.14

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.92 out of 39.62

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.05 out of 39.62

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.08 out of 26.85

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 23.00 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 5, location 2535196 - 2583564:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MG068_RS11965: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (457..845): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWELHFFTSGEHNLYGPTEAAVDVSY
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

MG068_RS11980: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (39..443): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SASLFPMSSPGGGLQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PMPAQGVVNK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
CatR	H2O2-responsive repressor
IolR	Regulator of the inositol catabolism
MntR	Manganese transport regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP031167.1 - Region 6 - terpene-precursor

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Location: 3,194,290 - 3,215,168 nt. (total: 20,879 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

MG068_RS15000: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000643.3	0.33	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.32	terpene	carotenoid	Brevundimonas sp. SD212
BGC0000640.3	0.32	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000630.3	0.31	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000641.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC413

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000643.3	0.33	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.32	terpene	carotenoid	Brevundimonas sp. SD212
BGC0000640.3	0.32	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000630.3	0.31	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000641.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC413

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

MG068_RS15000: PT_FPPS_like

Domain 1 (32 - 292): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

Additional weak hits (6):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.46 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.63 out of 18.57

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.42 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.54 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.56 out of 29.25

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein

NZ_CP031167.1 - Region 7 - arylpolyene

Download region GenBank file

Download region SVG

Location: 4,378,897 - 4,422,499 nt. (total: 43,603 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

MG068_RS20230: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

MG068_RS20300: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase halogenase

MG068_RS20320: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

MG068_RS20375: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: hydrolase

MG068_RS20250: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase synthase

MG068_RS20295: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MG068_RS20310: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MG068_RS20350: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

MG068_RS20355: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002008.3	0.63	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0000836.5	0.62	other (other)	APE Ec	Escherichia coli CFT073
BGC0002276.2	0.60	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001160.5	0.59	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001895.4	0.50	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0002000.4	0.49	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0001723.5	0.49	PKS	oviedomycin	Streptomyces antibioticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002008.3	0.63	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0000836.5	0.62	other (other)	APE Ec	Escherichia coli CFT073
BGC0002276.2	0.60	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001160.5	0.59	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001895.4	0.50	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0002000.4	0.49	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0001723.5	0.49	PKS	oviedomycin	Streptomyces antibioticus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.83 out of 20.47

Confidence: medium

Additional weak hits (10):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.07 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.17 out of 27.55

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.23 out of 27.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.00 out of 23.43

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.60 out of 29.25

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.52 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

MG068_RS20355: X

AMP-binding (53..300): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: THQ-----LFRWVAFSSGGTEVYGSSETGGIAWR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems