LOCUS       NZ_CP031167            10846 bp    DNA     circular CON 06-MAY-2024
DEFINITION  Stenotrophomonas sp. ASS1 chromosome, complete genome.
ACCESSION   NZ_CP031167
VERSION     NZ_CP031167.1
KEYWORDS    RefSeq.
SOURCE      Stenotrophomonas sp. ASS1
  ORGANISM  Stenotrophomonas sp. ASS1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Stenotrophomonas.
REFERENCE   1  (bases 1 to 4564481)
  AUTHORS   Elufisan,T.O., Guo,X., Rodriguez-Luna,I.C., Sanchez-Varela,A.,
            Villabos-Lopez,M.A. and Bustos,P.
  TITLE     Whole genome sequence analysis of hydrocarbon degrading
            Stenotrophomonas species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4564481)
  AUTHORS   Elufisan,T.O., Guo,X., Rodriguez-Luna,I.C., Sanchez-Varela,A.,
            Villabos-Lopez,M.A. and Bustos,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2018) Laboratorio de Biotecnologia Genomica, Cbg
            Instituto Politecnico Nacional, Boulevard del Maestro Esq Elias Pina
            la Escalera, Reynosa 88710, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-OCT-2017
            Assembly Method                   :: SPAdes v. 3.11.1
            Assembly Name                     :: ASS1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004346925.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 02:43:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,162
            CDSs (total)                      :: 4,082
            Genes (coding)                    :: 4,060
            CDSs (with protein)               :: 4,060
            Genes (RNA)                       :: 80
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 8 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:30:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1138861
            Orig. end                         :: 1149707
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031167.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Temidayo Oluyomi Elufisan.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10846
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10846
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10846
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5846](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5846)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            235..1773
                     /locus_tag="MG068_RS05280"
                     /old_locus_tag="MG068_05250"
     CDS             235..1773
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1273: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036331.1"
                     /locus_tag="MG068_RS05280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05250"
                     /product="TerC family protein"
                     /protein_id="WP_132809516.1"
                     /transl_table=11
                     /translation="MEWLADPSIWMGLATLVVLEIVLGIDNLVFIAILADKLPPHQRDR
                     ARVIGLALALLMRLVLLAALAWIMKLTEPLLTLFDHSFSGRDLILLGGGLFLLFKGTME
                     LHERLEGREHHEDGKKVYASFAMVVAQIVVLDAVFSLDSVITAVGMVDNLGVMYAAVTI
                     AMVLMLVASKPLTRFVNKHPTVVVLCLGFLLMIGFSLVAEGLGYKIPKGYLYAAIAFSI
                     LVEAFNQWVRFNRERNERRQPFRQRTADAVLRMLGARPANGHHDEEASEQVDAEERLQP
                     AEHEMIRSVLGLADRPVSSVMTVRADVQWIDLARGQEDVVARLVASPHTRLLVGDGDLD
                     SLRGVVQSRDLLADLLQGRPLQLEGNLREPQYVLSSASALQALELIRQHPVPLAVAVDE
                     YGSVEGLVTANDLLAAIAGDLVDTQDERYGVEAQGENQWEADGALTLDDLQRLAGVSLP
                     RSADYMTISGLVLEQLGRLPDIGDTVEIADVRITVLAMEKRRIARLRVERIGLS"
     gene            1907..3043
                     /locus_tag="MG068_RS05285"
                     /old_locus_tag="MG068_05255"
     CDS             1907..3043
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Metallophos_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004147841.1"
                     /locus_tag="MG068_RS05285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05255"
                     /product="metallophosphoesterase"
                     /protein_id="WP_049461802.1"
                     /sec_met_domain="Metallophos_2 (E-value: 1e-08, bitscore:
                     33.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVAVLSTIGLLMGLYVAWRLFWPLRMPIWMKVVLSLLLVSVAVQL
                     RIVAMFWGTMASPEIPKLAIASLATGSTAVLLLALFLLVLDAGLLVSRILRRPRAVSAL
                     RAPKLRPAAGVLALLVSAYGVSQGMAVPKPRHIEVSIAGLPAGFDGYRVLQLTDIHASR
                     LLTGDWVRRVVEESNALKPDLIVITGDLIDGSVEARRDDARPLADLQAPDGVIAITGNH
                     EYYAQYQEWMQAFRALHMQVLENSHLQVRRGDAALTIAGVTDPVAARYGLPLPDLEAAL
                     AGADPGAPVILLDHRPRNAREAAARGVKLQLSGHTHGGQIIGMDQLVKRANGGFVSGRY
                     EVDGMTLYVSNGAGLWAGFPARIGVPSEITLITLRRAP"
     gene            3116..3520
                     /locus_tag="MG068_RS05290"
                     /old_locus_tag="MG068_05260"
     CDS             3116..3520
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510383.1"
                     /locus_tag="MG068_RS05290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05260"
                     /product="hypothetical protein"
                     /protein_id="WP_132809518.1"
                     /transl_table=11
                     /translation="MSHPRAPMERLIRANADEINRLRQAIREAAGACWRGPEAMQRHVA
                     ACAEYNQRYEQLAFPGGYANALKQLAERDPDTVDVVLTFLEVRPYFFRSGYMWKTLLKR
                     VQRAPMGVKQQARMQKILDAYAAYRATRDG"
     misc_feature    complement(3138..3152)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.77"
                     /tool="antismash"
     gene            complement(3584..4222)
                     /locus_tag="MG068_RS05295"
                     /old_locus_tag="MG068_05265"
     CDS             complement(3584..4222)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408497.1"
                     /locus_tag="MG068_RS05295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05265"
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="WP_049422757.1"
                     /transl_table=11
                     /translation="MAASNTPFLHGWRLFVSVAVVLIAFALAAFALYPDAAEGSRAAIR
                     VTARTSFLLFLAAFTASSFASLLPSPFTRFLLRERRIIGLSFAFSHLLHAIAITSFGIL
                     NPAFWPARSALANLPGTVGYIAILALAITSHRGLARRMGPTAWRRLHVTGMWIIAAVFT
                     YSYFKRVPGNFWYAVPSALLFTAFVVRWIAKRAQALRRSAHARPPLRAA"
     gene            complement(4274..5008)
                     /locus_tag="MG068_RS05300"
                     /old_locus_tag="MG068_05270"
     CDS             complement(4274..5008)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646320.1"
                     /locus_tag="MG068_RS05300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05270"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_049461799.1"
                     /transl_table=11
                     /translation="MAESLATLQRRWADHVRDPAKTAPDGIEARRMAVYRRLCIDSLDT
                     LLASSLPRLQQQLGTSRWRDIVEHYYASHICHTPLFPRIAGEFAAWLAVQDTLALPGWA
                     AELAHYESTQQSLHIETRDGGRPLQRAPVGNDVLAVSPLVRVLGYQWPVHEDGALEPVL
                     STAPTLLVIQRLADFSLQTEELSPPAYALLSVFGDDSASVDEALQLLSETHGEAADALR
                     IACVPVLGELCAAGVLVALPAF"
     gene            complement(5001..5846)
                     /locus_tag="MG068_RS05305"
                     /old_locus_tag="MG068_05275"
     CDS             complement(5001..5846)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646321.1"
                     /locus_tag="MG068_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05275"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_049422759.1"
                     /sec_met_domain="DUF692 (E-value: 2.8e-119, bitscore:
                     395.2, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLPLPSNAAGLGLRRGLIDELLAMPAGAIDFLEVSPDNWIGVGG
                     AHGAALRRLSERHPLTCHGLSLSLGGPDPLDRTLLAQTRAFLDLHQVQLYSEHLSYCAA
                     GGHVYDLLPLPFTAEAVHHIAGRIAQVQDMLGRRIAVENISYYAVAGADMSEIDFINAV
                     LAEADCDLLLDVNNVFVNACNNGYDAFEFLARVPAHRVASLHVAGHFDEDDGFKIDTHG
                     APVKGVVWALLREAYARVGVRPTLLERDFNFPPLAELLAEVGQIRQAQAEARWPEVAHG
                     "
     gene            complement(5903..6379)
                     /locus_tag="MG068_RS05310"
                     /old_locus_tag="MG068_05280"
     CDS             complement(5903..6379)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412584.1"
                     /locus_tag="MG068_RS05310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05280"
                     /product="EF-hand domain-containing protein"
                     /protein_id="WP_132809519.1"
                     /transl_table=11
                     /translation="MSVSTKNTPSSRLPRIGAIGIALAGGLLLAGQAAAIQPLAVSAMA
                     MSAAAEGKCGEGKCGANKTATGKPAGKAKVAEGQCGEGKCGDASFARTDRDHDGRVSRA
                     EFNAVAAKRAAEFDRIDTDHDGYISEQEAHDYLRSVYTANGKPMPKGLFSRVAD"
     gene            complement(6480..7124)
                     /locus_tag="MG068_RS05315"
                     /old_locus_tag="MG068_05285"
     CDS             complement(6480..7124)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479418.1"
                     /locus_tag="MG068_RS05315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05285"
                     /product="DoxX family protein"
                     /protein_id="WP_132809521.1"
                     /transl_table=11
                     /translation="MISTASSVYTPRLDAVGRWLSPLALRTLLAWEFFESGREKLGGQN
                     WFADLEGRFPFPFSTLPASLNWQLATWLELVGAVMLLLGLATRSVAYVFWVLTVVAIAA
                     VHWPDQWNGLGELWQGYAITDQGYGNFKLPLLFLAMLLPLILNGGGALSVDRLLAGSQH
                     APVGNDGLGWGVALIALLLPVAALLPGIGFGGALLGGVLLVGYVLRRRRNA"
     misc_feature    complement(7228..7246)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.64"
                     /tool="antismash"
     gene            7467..8261
                     /locus_tag="MG068_RS05320"
                     /old_locus_tag="MG068_05290"
     CDS             7467..8261
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408502.1"
                     /locus_tag="MG068_RS05320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05290"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_132809522.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.3e-14, bitscore:
                     53.2, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTTLLAAALALAGAAAPAFAAQVDAGAPPTIILVHGAFADGSS
                     WSKVISTLHDWKLPAVAVQNPLTSLADDVAATRRAIAAAPGKVVLVGHSWGGTVITEAG
                     DDPKVQALVYVAAFAPDAGQSSAQQGEGFPVGPGLARLQERDGYLTLPADAIAQDFAPD
                     VMKKSAALLYSTQVPLKASALGEVVNVAAWRSKPSWYVLSRDDRMLSPQLQAATARRIG
                     AQLQSIGSSHVSLLSHPAQVADSILEAAGVKPAELPLAEQGG"
     gene            8265..8810
                     /locus_tag="MG068_RS05325"
                     /old_locus_tag="MG068_05295"
     CDS             8265..8810
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1241: lipoprotein
                     dsbF"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415663.1"
                     /locus_tag="MG068_RS05325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05295"
                     /product="redoxin family protein"
                     /protein_id="WP_132809524.1"
                     /transl_table=11
                     /translation="MAALRGYALPLLLGLLGGAALLPAWPSAEAGAQPLDAAPKAGFGD
                     GGPWHNSPPMTLKQLQGQVVLVEFWTYTCSNCLNVAPYVHQWHARYASQGLRVIGVHTP
                     EFAYEGLPGNVRKAIARLDITWPVVQDNQYRIWNAWGNRFWPALYLLDRQGRVVYRHYG
                     EGDYARTESEIQRQLASP"
     gene            8869..9609
                     /locus_tag="MG068_RS05330"
                     /old_locus_tag="MG068_05300"
     CDS             8869..9609
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659345.1"
                     /locus_tag="MG068_RS05330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MG068_05300"
                     /product="response regulator transcription factor"
                     /protein_id="WP_049461794.1"
                     /transl_table=11
                     /translation="MNPVPHILVVDDDSDIRQMLADYLQRNGLRVSQADGGRAMRALMD
                     THAVDLVVLDVMMPGEDGLSLCRNLRAGKHRAVPVVLLTARDDETDRIIGLEMGADDYV
                     TKPFSSRELLARINAVIRRTRMLPPNLQVSEAGRQLAFGAWRLDTTARHLLDAQDTAYP
                     LSGAEFRLLRVFLDHANRVLSRDQLLSLTQGRDAELFDRSIDLLVSRVRQRLGDDAREP
                     TYIKTVRSEGYVFSVPVQLLGPDE"
     misc_feature    complement(10405..10419)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    complement(10698..10712)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 aggaacgcgg catgaccctg tccgagctgg ccgggcgcat cgacatcacc ctggccaatc
       61 tgtcgatcct gaagaccggc aaggcgcgcg ccatccgctt ttccaccctt gatgcgatct
      121 gccgcgagct gcagtgcacg cccggcgacc tgctcgggca cgacccggcg caggcacagg
      181 acgctgactg agcggtcgct cttttccctt acctactgac gaaacggacc tgaaatggaa
      241 tggttggctg acccctcgat ctggatgggc cttgcaaccc tggtcgtgct ggagatcgtt
      301 ctcggcatcg acaacctggt gttcatcgcg atccttgccg acaagctacc gccgcaccag
      361 cgtgaccgcg cgcgggtaat cggcctggcg ctggccttgc tgatgcggct ggtgctgctg
      421 gcggcgttgg cgtggatcat gaaactgacc gagccgctgc tgacgctgtt cgaccacagc
      481 ttctccgggc gtgacctgat cctgctgggc ggtggtctgt tcctgctgtt caagggcacg
      541 atggaactgc acgagcggct ggagggccgc gagcatcacg aggatggcaa gaaggtctac
      601 gccagcttcg cgatggtggt ggctcagatc gtggtgctcg acgcggtgtt ctcgctggac
      661 tcggtgatca ccgcggtcgg catggtcgac aacctgggcg tgatgtacgc cgcggtgacc
      721 atcgcgatgg tgctgatgct ggtggccagc aagccgctga cccgcttcgt gaacaagcat
      781 cccacggtgg tggtgctgtg cctgggcttc ctgttgatga tcggtttcag cctggtggcc
      841 gaaggcctgg gctacaagat cccgaagggt tatctgtacg cggcgatcgc cttctcgatc
      901 ctggttgaag ccttcaacca gtgggtgcgc ttcaatcgcg agcgcaacga gcgtcgccag
      961 ccattccgtc aacgtactgc cgacgccgta ctgaggatgc tcggcgcgcg gccggccaac
     1021 ggccatcacg acgaagaggc cagcgagcag gtggatgctg aagagcgcct gcagccggcc
     1081 gagcacgaga tgatccgcag cgtgctgggc ctggctgatc gcccggtgtc gagcgtgatg
     1141 accgtgcgtg ccgacgtgca gtggattgac ctggcacgtg ggcaggagga tgtggtggca
     1201 cgcctggtgg catcgccgca cacccgcctg ctggtgggtg atggcgacct ggacagcctg
     1261 cgcggcgtgg tgcagagccg cgaccttctg gccgacctgc tgcagggcag gccgctgcag
     1321 ctggaaggca acctgcgcga gccgcagtac gtgctgtcca gcgccagcgc actgcaggcg
     1381 ctggaactga tccgccagca cccggtgccg ttggcggtag cggtggacga gtacggcagt
     1441 gtggaaggtc tggtgaccgc caacgatctg ctggcggcca ttgccggtga cctggtcgac
     1501 acccaggacg agcgctatgg cgtggaggcg cagggcgaga accagtggga ggccgacggt
     1561 gcgctgaccc tggacgacct gcagcgcctg gccggcgtgt cgctgccgcg cagtgccgac
     1621 tacatgacca tctccggact ggtactggag cagttgggtc gcctgccgga catcggtgac
     1681 acggtggaga ttgcggatgt acgcatcacc gtactggcaa tggagaagcg tcgcatcgcc
     1741 cgcctgcggg tggagcgcat cgggctttcg tagagtcgag cctgctcgac tggccctctg
     1801 tagagtcgag ccatgctcga ctgcttcctg agcagccggg tacgggcccg gctctacaca
     1861 acactgccgg caaccgtcac catggcgggc ccgctttctt tccaggatgg ttgcagtgct
     1921 gagtacgatc ggtttgttga tgggcctgta tgtggcctgg cgcctgttct ggccactgcg
     1981 catgcccatc tggatgaagg ttgtgctgtc gctgctgctg gtgagcgttg ccgtgcagct
     2041 gcggatcgtc gccatgttct ggggcacgat ggcctcgccg gaaatcccga agctggccat
     2101 cgcctcgttg gccacgggtt cgactgccgt actgctgctg gcgctgttcc tgctagtgct
     2161 ggacgcgggc ctgttggtgt cgcgcatcct gcgcaggcca cgtgctgtat cggcgttgcg
     2221 cgcgccgaag ctgcgcccgg cggcgggtgt gctggcgctg ctggtcagcg cctatggcgt
     2281 cagccaggga atggcggtgc ccaagcccag gcacatcgag gtgagcatcg ccgggttgcc
     2341 ggccgggttc gacggctatc gcgtgctgca gctgaccgac atccatgcca gccgcctgct
     2401 gaccggcgac tgggtgcgca gggtggtgga ggagagcaac gcactgaagc cggacctgat
     2461 cgtgatcact ggcgatctga tcgacggcag tgtcgaggcc cgtcgcgacg acgcgcgtcc
     2521 gttggccgac ctgcaggcgc cggatggcgt cattgccatc accggcaatc acgagtacta
     2581 cgcgcagtac caggagtgga tgcaggcgtt ccgcgcactg cacatgcagg tgctggagaa
     2641 cagccacctg caggtgcggc gcggcgatgc ggcgctgacc atcgccggtg tcaccgatcc
     2701 ggtggccgca cgctacggtc tgccgctgcc ggacctggag gccgcgctgg ccggtgctga
     2761 tccgggcgcg ccggtgatcc tgctcgacca tcgcccgcgc aacgcacgcg aggcggccgc
     2821 gcgtggggta aagctgcagt tgtccgggca tacacatggc ggccagatca tcggcatgga
     2881 ccagctggtg aagcgcgcca atggcggttt tgtgtccggc cgctacgagg tggatggcat
     2941 gacactgtac gtgagcaatg gcgccggcct gtgggccggt ttcccggcac gcatcggcgt
     3001 tccttcggag atcaccctga tcaccctgcg ccgcgccccg tgacctgaag tggggtcaga
     3061 gccctttgcc tgcgggaaat ggatctgacc cctgacaaac caaatggaag gaaccatgtc
     3121 ccatccccgc gcacccatgg aacgtcttat ccgcgccaat gcggacgaga tcaatcggct
     3181 gcgccaagcc atccgcgaag ccgctggcgc gtgctggcgt ggcccggaag caatgcagcg
     3241 gcacgtggca gcctgcgcgg aatacaacca gcgctatgaa cagcttgctt tcccgggggg
     3301 ctacgccaac gcgctgaagc aacttgccga acgcgatcca gatacggtgg atgtcgtgct
     3361 gaccttcctt gaggtgcgcc cgtacttctt ccgttccgga tatatgtgga agacgctgct
     3421 gaagcgcgtg cagcgggcac cgatgggtgt gaagcagcag gcccggatgc agaagatcct
     3481 cgatgcctac gctgcctatc gcgccacacg tgacggctga tggggacgac atgatggggt
     3541 cagatccctt cgcctttggc aaagggatct gacccccggc ccgtcaggcc gctcgcagcg
     3601 gcggccgcgc atgcgcactt cgacgcagcg cttgcgcccg cttggcgatc cagcgcacca
     3661 cgaaggcggt gaacagcagc gccgacggca ccgcatacca gaagttgccc ggcacgcgct
     3721 tgaagtacga gtaggtgaaa accgccgcga tgatccacat gccggtgacg tgcaggcgac
     3781 gccaggccgt gggtcccatg cgccgggcga gcccccgatg cgaggtgatg gccagcgcca
     3841 ggatggcgat gtagcccacc gtgcccggga ggttggccag cgctgagcgt gctggccaga
     3901 atgccgggtt gaggatgccg aaactggtga tggcgatggc atgcagcagg tgcgagaacg
     3961 cgaaggacag gccgatgatg cgccgttcgc gcagcaggaa ccgggtgaac gggctgggca
     4021 gcaggctggc gaacgacgat gccgtgaacg ctgccaggaa caacaggaac gatgtgcgtg
     4081 cagtgacccg gatcgcggct cggctgcctt cggccgcatc gggatacagg gcgaaggccg
     4141 ccagcgcgaa ggcgatcagg accaccgcca cggacacgaa caggcgccag ccgtgcagga
     4201 agggagtatt ggatgcagcc atggtgagac ctcgaagtag gggggcggta gtgccggccg
     4261 ctggccggca atctcagaaa gcaggcaatg cgaccagcac gcccgccgcg cacagctcgc
     4321 ccagaacagg cacgcaggca atgcgcaatg cgtccgctgc ctcgccatgt gtttcggaca
     4381 gcagctgcag tgcttcatcc accgacgcgc tgtcgtcacc gaacaccgac agcagcgcgt
     4441 aggccggcgg tgacagttcc tcggtctgca ggctgaaatc ggcgaggcgc tggatcacca
     4501 gcagcgtggg agccgtgctc aacaccggct ccagcgcccc gtcctcatgt actggccact
     4561 ggtagccaag cacccgcacc aacggcgaaa ccgccagcac atcattgcct acgggagcgc
     4621 gctgcagcgg ccgcccgccg tcacgcgttt caatgtgcag cgactgctgc gtgctctcgt
     4681 agtgcgccag ttctgctgcc cagcccggca gtgccagcgt gtcctgcact gccagccagg
     4741 ccgcgaactc gccggcgatc cgtgggaaca acggggtgtg acagatgtgg ctggcgtagt
     4801 agtgctccac gatgtcgcgc cagcgcgacg tgcccaactg ttgctgcagt cgcggcaggc
     4861 tgctggccag cagggtgtcc aagctgtcga tgcacaggcg gcggtagacc gccatccgcc
     4921 gcgcctcgat gccatcgggt gcggtctttg cagggtcgcg cacatgatcg gcccagcgcc
     4981 gttgcagggt cgccagcgac tcagccatgg gccacctcgg gccaacgtgc ctcggcctgc
     5041 gcctggcgga tctgcccgac ctcggccagc aattcggcaa gcggggggaa gttgaagtcg
     5101 cgttcgagca gcgtggggcg aacgcccacc cgcgcatagg cctcgcgcaa cagtgcccag
     5161 accacgcctt tcaccggtgc accatgggta tcgatcttga agccatcgtc ttcatcgaag
     5221 tggcccgcca catgcagcga ggcgacgcgg tgggccggca cgcgggccag gaattcgaag
     5281 gcgtcataac cattgttgca ggcgttgacg aaaacgttgt tgacgtccag cagcaggtcg
     5341 cagtcggctt cggcgagcac ggcgttgatg aagtcgatct cgctcatgtc cgcgccggca
     5401 acggcgtagt aggagatgtt ctccacggcg atgcgtcggc ccagcatgtc ctgcacctgg
     5461 gcgatgcgac cggcgatatg gtgaacggcc tcggcggtga acggcagcgg cagcaggtcg
     5521 tagacgtggc caccggcagc acagtagctc aggtgttcgc tgtacagctg cacctggtgc
     5581 aggtcgagga aggcacgcgt ctgggccagc agggtgcggt ccagcggatc aggaccgccc
     5641 aaagaaagcg acaggccgtg gcaggtcagg gggtggcgtt cgctcaacct gcgcagcgct
     5701 gcaccgtgtg caccgccgac gccgatccag ttgtcgggcg aaacttcgag gaagtcgatg
     5761 gcaccggccg gcatggccag cagttcgtcg atcaggccac ggcgcaggcc aagaccggcg
     5821 gcattggaag gcaagggcag ggtcatgacg gatgctccag ggagagggcg ccgcctcgga
     5881 ggcggcgccg gttgaacgtc gatcaatcgg cgacgcggga gaacaggccc ttcggcatcg
     5941 gcttgccgtt ggcggtgtag acgctgcgca ggtagtcgtg tgcctcctgc tcgctgatgt
     6001 agccgtcgtg gtcggtgtcg atgcggtcga actcggcggc acgcttggcg gccacggcgt
     6061 tgaactcggc acgcgagacc cggccatcgt ggtcgcggtc ggtgcgggca aacgaggcgt
     6121 cgccgcactt gccttcgcca cactggccct cggccacctt ggccttgccg gcgggcttgc
     6181 cggtggcggt cttgttggct ccgcacttgc cttcaccgca cttgccttcg gcagcagcgc
     6241 tcatggccat ggcactgacc gccagcggct ggatcgccgc ggcctggccg gccagcagca
     6301 ggccaccggc cagggcgatg ccgatggcac cgatgcgcgg caggcgggag gagggggtgt
     6361 tcttggtcga gacggacatg gtggtgactc cttgggaaag cacgcggggc gtgcgggatg
     6421 gacgccgctt gaaggcgacg gaaacgaagg gaagaagcgc ggtgaggggg cgaagcggat
     6481 caggcgttgc ggcgacggcg cagtacgtaa cccaccagca gcacaccgcc gagcagggcg
     6541 ccgccgaagc cgatgccggg cagcagcgcg gcaaccggca gcagcagggc gatgagggcg
     6601 acgccccaac ccaagccgtc attgccaacg ggggcgtgct gcgagccggc cagcagtcgg
     6661 tccacgctga gtgcgccgcc gccgttgagg atcagtggta gcagcatggc caggaacagc
     6721 agcggcagct tgaagttgcc gtagccctgg tcggtgatcg catagccctg ccagagttcg
     6781 cccaggccgt tccattggtc gggccagtgc acggcggcga tggcgaccac ggtcagcacc
     6841 cagaacacat aagccaccga gcgcgtggcc aggccgagca gcagcatcac cgcacccacc
     6901 agttccagcc aggtggccag ctgccagttc agtgatgcgg gcagggtgga gaacgggaac
     6961 gggaagcgac cttccaggtc ggcgaaccag ttctggccgc ccagcttttc gcggccggat
     7021 tcaaagaact cccaggccag cagggtgcgc agcgccaatg gcgaaagcca gcggccgacg
     7081 gcgtccaggc ggggggtgta cacggaagag gcggtgctga tcatggtggt gtcctgcggg
     7141 ccagtggtcg atggaggcca tttcaggcgg cgcaggtatc ggcaacgtgt gccgttgccc
     7201 ggggttttgt cagcgactgt ggccgcggcc ggggtttcgt acagtcagat acaaagacga
     7261 tgcgttggtg cacatcgggg ccttgcacgc ccgcgtgcgg ggagcgatat ccacccgtta
     7321 gcgcccagcc aggaccggcg cccgaatgta tcccactgta gaaacccgcc tgcgccctac
     7381 agtccggtac aactccgtgc cgcggcgaaa cagcgccgat acaccggcag gcggaaatgg
     7441 ccgctcccaa caccggagtg ccactcatga tccgtaccac gttgcttgcc gccgctctcg
     7501 cccttgccgg cgccgccgcc ccggccttcg ctgcccaggt cgacgccggc gcgccgccca
     7561 ccatcatcct ggtgcacggc gcgttcgccg atggctccag ctggagcaag gtcatcagta
     7621 ccctgcatga ctggaagctg ccggcggtgg ccgtacagaa tccgctcact tcgctggccg
     7681 acgatgttgc cgccacccgc cgtgcgattg ccgctgcacc cggcaaggtg gtgctggtcg
     7741 gccacagctg gggcggcacg gtcatcaccg aagcgggcga tgacccgaag gtgcaggcgc
     7801 tggtgtacgt ggccgcgttc gcaccggatg ccgggcagtc atcggcacag cagggcgagg
     7861 gcttcccggt cggtccgggc ctggcccgac tgcaggagag ggatggctac ctgaccttgc
     7921 ctgccgatgc gatcgcccag gacttcgcgc cggacgtgat gaagaagtcg gctgcgctgc
     7981 tgtacagcac ccaggtcccg ttgaaggcca gcgcgctggg cgaggtggtg aacgttgcgg
     8041 cgtggcgcag caagccgagc tggtacgtgc tcagccgcga tgaccgcatg ctgtcgccgc
     8101 agctgcaggc cgccaccgca cgccgcatcg gcgcacagct gcagtcgatc ggcagcagcc
     8161 acgtgtcgct gctttcgcat ccggcgcagg tggccgacag catcctggag gcggcaggcg
     8221 tgaagccggc cgaactgccg ctggccgagc aggggggctg agcgatggcc gcactgcgag
     8281 ggtatgcact gcccctgctg ctgggcctgc tgggtggagc ggcgttgctg ccggcctggc
     8341 ccagcgccga ggccggcgcg cagccactcg acgcagcacc gaaggcgggg ttcggcgatg
     8401 gcgggccctg gcacaacagc ccaccgatga cactgaagca gttgcagggg caggtggtac
     8461 tggtcgagtt ctggacctac acctgcagca actgcctcaa cgtggcgcca tacgtccacc
     8521 agtggcatgc acgctacgcg tcgcagggcc tgagggtgat cggcgtgcat acgcccgagt
     8581 tcgcctatga agggctgccc ggcaacgtgc gcaaggccat cgcacggttg gatatcacct
     8641 ggccggtggt gcaggacaac cagtaccgga tctggaacgc gtggggcaac cgcttctggc
     8701 ccgcgctgta cctgctcgac cggcaggggc gggtggtcta ccgccattac ggcgagggcg
     8761 actacgcacg caccgaaagc gagatccagc gccagctggc cagcccctga gccgcgctac
     8821 catggccgcg ccgcgcggcg ctgtccccct gccttcatcg agaacgcaat gaaccctgta
     8881 ccgcacatcc tggtcgtcga cgatgacagc gatatccgcc agatgctggc cgactacctg
     8941 caacgcaacg gcctgcgcgt gagccaggcc gacggtggcc gggccatgcg cgcgctgatg
     9001 gatacccatg ccgtggatct ggtggtgctg gacgtgatga tgccaggcga ggacggcctg
     9061 agcctgtgcc gcaacctgcg cgccggcaag catcgtgcgg tgccggtggt gctgctgacc
     9121 gcccgcgacg acgagaccga ccgcatcatc ggcctggaaa tgggcgctga cgactacgtg
     9181 accaagccct tttcctcgcg cgaactgctg gcacgcatca acgcggtgat ccgtcgcacc
     9241 cggatgctgc cgccgaacct gcaggtgagc gaagccggtc gccaactggc cttcggtgcc
     9301 tggcgcctgg ataccaccgc gcgccatctg ctggatgcgc aggacaccgc gtatccgctc
     9361 agtggtgccg agttccgcct gttgcgcgtg ttcctcgatc acgccaatcg ggtgctcagc
     9421 cgtgaccagc tgctcagcct cacccagggc cgcgatgccg aactgttcga ccgctcgatc
     9481 gacctgttgg tcagccgcgt gcggcagcgc ctgggtgacg acgcacgcga gccgacctac
     9541 atcaagaccg tgcgcagcga aggctatgtg ttcagcgtgc cggtgcagtt gctggggccg
     9601 gacgaatgaa caccaccgca cgccgtccac gctggccgcg cacgctgtcg gcgcggctgc
     9661 tgctggtgtt gctgggcgga ctggccctgg cccacgcact gtccttcggc ctgctgttct
     9721 tcgaacgcta ccagtccacc cgcagcatga tgctgcgcaa cctcgacgag gatgtggcgg
     9781 tgagtgtcgc gctgctggag cacctgcctg tggcgcagcg tgaggcctgg gtgccgcggc
     9841 tggagcggcg cacctaccgc tacctgctgc gcccggcttc ggccgggccg gggctgcaga
     9901 ccgatcgtgc acgccaggtc acggcgatca tcgacgacag cctgcagcac cgctatccgt
     9961 tgcaggcgcg ccaggtggct cgcctgccgg agcggttcga ggtggaactg cgcctgcacg
    10021 acggcacacc cttgaccatt gaagtcactc cgtcgggcct gccgctggca cgttggctgc
    10081 ccgccgtgct gctggtgcag ctggcgctgc tgctggtgtg cgcgtggttg gcggtgcgcc
    10141 tggccatgcg cccgctgcag cagctgtcgc atgcggtgga gcatctgcag ccgggcaagg
    10201 acgggccggc actgcctgaa gatggtcctg cggaagtagg cggtgcggcg gccgcactca
    10261 acgcgctgca ggcacgcatt cgtggccatg tcagtgagcg cctgcagatc ctggccgcga
    10321 tctcgcacga cctgcagacc cccatcacgc gcatgaagct ccgggtggag accctgcctg
    10381 aagacagcac ccagcagcgg ctgctggccg acctcgacca cctggggcaa ctggtgcgtg
    10441 agggcgtggc ctatgcgcgc agcagccacg tcgccagtgg cgcgccggtg tcgatggacc
    10501 tgggcgcgtt cctggccagt gtggtcggcg attacgagga catgggcaag ccggtcagtg
    10561 gtggtgcgcc cgctggactg gtcgtgcaga cctggccgca gccgctgcgc cgggtggtcg
    10621 gcaatctggt cgacaacgcg ttgcgctacg ccggtgccgc cgagatcgag gcgggccgcg
    10681 atgaggccgg aagggtgtgg atcggaattt ccgaccgtgg ccccggcatt cccgagggcc
    10741 agctgcaggc agtgctggcg ccgttccacc ggctggaaag ctcgcgcaat cgcgataccg
    10801 gcgggaccgg cctgggcctg gccatcgcgg tgcagctggc gcagtc
//
