GCF_004345665.1 - 15 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_SLWR01000001.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	NI-siderophore	567,935	601,088	High	speibonoxamine/desoxy-desferrioxamine D1/desferrioxamine D1/desferrioxamine B/didesoxy-desferrioxamine D1/desoxy-desferrioxamine B/didesoxy-desferrioxamine B	other:other
Region 1.2	terpene-precursor	937,574	958,644
Region 1.3	NRPS,T1PKS	1,256,173	1,298,168

NZ_SLWR01000002.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 2.1	lanthipeptide-class-iv	355,884	378,541
Region 2.2	lanthipeptide-class-iii	438,995	461,535	Medium	catenulipeptin	ribosomal:RiPP:Lanthipeptide

NZ_SLWR01000003.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	hydrogen-cyanide	45,243	58,163
Region 3.2	lanthipeptide-class-ii	76,629	99,343
Region 3.3	lanthipeptide-class-iii	103,791	126,358	Medium	catenulipeptin	ribosomal:RiPP:Lanthipeptide
Region 3.4	lincosamides,arylpolyene	548,028	594,475

NZ_SLWR01000006.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	azole-containing-RiPP	440,088	462,735

NZ_SLWR01000008.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	NI-siderophore	78,447	108,206	Low	bisucaberin B	other:other

NZ_SLWR01000009.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	T1PKS,hglE-KS	127,980	179,216

NZ_SLWR01000011.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 11.1	NAPAA	343,545	377,474	High	ε-Poly-L-lysine	NRPS:Type I

NZ_SLWR01000017.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 17.1	terpene	26,604	51,200

NZ_SLWR01000019.1 (Kribbella antiqua)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 19.1	T3PKS	16,804	57,850	High	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone/2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	PKS:Type III

No secondary metabolite regions were found in these records:: NZ_SLWR01000004.1 (Kribbella antiqua); NZ_SLWR01000005.1 (Kribbella antiqua); NZ_SLWR01000007.1 (Kribbella antiqua); NZ_SLWR01000010.1 (Kribbella antiqua); NZ_SLWR01000012.1 (Kribbella antiqua); NZ_SLWR01000013.1 (Kribbella antiqua); NZ_SLWR01000014.1 (Kribbella antiqua); NZ_SLWR01000015.1 (Kribbella antiqua); NZ_SLWR01000016.1 (Kribbella antiqua); NZ_SLWR01000018.1 (Kribbella antiqua); NZ_SLWR01000020.1 (Kribbella antiqua); NZ_SLWR01000021.1 (Kribbella antiqua); NZ_SLWR01000022.1 (Kribbella antiqua); NZ_SLWR01000023.1 (Kribbella antiqua); NZ_SLWR01000024.1 (Kribbella antiqua); NZ_SLWR01000025.1 (Kribbella antiqua); NZ_SLWR01000026.1 (Kribbella antiqua); NZ_SLWR01000027.1 (Kribbella antiqua); NZ_SLWR01000028.1 (Kribbella antiqua)

Compact view

NZ_SLWR01000001.1 - Region 1 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 567,935 - 601,088 nt. (total: 33,154 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

EV646_RS02880: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Transferases: kinase methyltransferase

EV646_RS02850: methyltransferase

smcogs: SMCOG1089: methyltransferase

EV646_RS02860: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Hydrolases: amidase hydrolase

EV646_RS02870: amidase

smcogs: SMCOG1105: amidase

EV646_RS02925: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: decarboxylase

EV646_RS02895: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.73	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001478.5	0.62	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.60	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002469.2	0.59	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0001531.5	0.58	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002305.2	0.56	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0001453.5	0.56	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000940.5	0.56	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0000244.4	0.54	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0002690.2	0.53	other (other)	FW0622	Verrucosispora sp. FIM060022

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.73	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001478.5	0.62	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.60	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002469.2	0.59	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0001531.5	0.58	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002305.2	0.56	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0001453.5	0.56	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000940.5	0.56	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0000244.4	0.54	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0002690.2	0.53	other (other)	FW0622	Verrucosispora sp. FIM060022

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.28 out of 26.33

Confidence: medium

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 24.24 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.98 out of 24.78

Confidence: medium

Additional weak hits (4):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.32 out of 26.12

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.23 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.06 out of 27.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.69 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response

NZ_SLWR01000001.1 - Region 2 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 937,574 - 958,644 nt. (total: 21,071 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

EV646_RS04545: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

EV646_RS04595: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

Transferases: phosphorylase

EV646_RS04590: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

Ligases: synthetase

EV646_RS04565: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.44	terpene	carotenoid	Rhodobacter sphaeroides
BGC0001364.3	0.43	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0000664.5	0.43	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.43	terpene	isorenieratene	Streptomyces argillaceus
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000633.3	0.42	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.42	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000648.3	0.40	terpene	carotenoid	Myxococcus xanthus
BGC0000644.3	0.40	terpene	carotenoid	Dietzia sp. CQ4
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.44	terpene	carotenoid	Rhodobacter sphaeroides
BGC0001364.3	0.43	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0000664.5	0.43	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.43	terpene	isorenieratene	Streptomyces argillaceus
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000633.3	0.42	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.42	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000648.3	0.40	terpene	carotenoid	Myxococcus xanthus
BGC0000644.3	0.40	terpene	carotenoid	Dietzia sp. CQ4
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

EV646_RS04565: PT_FPPS_like

Domain 1 (44 - 349): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.35 out of 20.47

Confidence: strong

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 25.27 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.07 out of 24.78

Confidence: medium

Additional weak hits (6):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 25.75

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.59 out of 36.54

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.36 out of 30.97

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.42 out of 36.54

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.08 out of 21.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.10 out of 20.97

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response
VqsM	Regulator of quorum-sensing signalling systems

NZ_SLWR01000001.1 - Region 3 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 1,256,173 - 1,298,168 nt. (total: 41,996 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: reductase

EV646_RS06050: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

Transferases: transketolase

EV646_RS06060: transketolase

smcogs: SMCOG1204: transketolase

Hydrolases: glucosidase

EV646_RS05985: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Isomerases: mutase

EV646_RS05970: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: synthase

EV646_RS06040: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.31	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000889.5	0.26	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0001810.3	0.25	PKS	trehangelin	Polymorphospora rubra
BGC0000264.5	0.23	PKS	resistomycin, resistoflavine	Streptomyces resistomycificus
BGC0000503.5	0.23	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000679.5	0.23	terpene	diazepinomicin	Micromonospora sp. M42
BGC0000197.5	0.23	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0001510.5	0.22	other (other)	anisomycin	Streptomyces hygrospinosus
BGC0000528.5	0.22	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.22	ribosomal	cebulantin	Saccharopolyspora cebuensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.46	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000889.5	0.42	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0001810.3	0.41	PKS	trehangelin	Polymorphospora rubra
BGC0000264.5	0.39	PKS	resistomycin, resistoflavine	Streptomyces resistomycificus
BGC0000503.5	0.39	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000679.5	0.39	terpene	diazepinomicin	Micromonospora sp. M42
BGC0000197.5	0.38	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0001510.5	0.38	other (other)	anisomycin	Streptomyces hygrospinosus
BGC0000528.5	0.38	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.37	ribosomal	cebulantin	Saccharopolyspora cebuensis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.36 out of 20.97

Confidence: medium

Additional weak hits (11):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.13 out of 39.62

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.55 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.64 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.03 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.88 out of 28.14

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.37 out of 30.22

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.59 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.77 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.68 out of 27.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.27 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.67 out of 26.06

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 1256172 - 1298168:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

EV646_RS06040: X - mal - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (44..477): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGTS--AFLLYLWSVSGGEVAAWGMTETVTGITF
Nearest Stachelhaus code(s):: Ppa VALFVGAAGK (50% 8Å match); 3clLeu GILWVGAGGK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

PKS_AT (2164..2448): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 120.4
Methylmalonyl-CoA: 61.4
inactive: 56.6
Methoxymalonyl-CoA: 45.6
CHC-CoA: 32.4
Propionyl-CoA: 32.2
Ethylmalonyl-CoA: 30.4
Benzoyl-CoA: 23.5
2-Methylbutyryl-CoA: 23.3
fatty_acid: 22.1
trans-1,2-CPDA: 21.2
Isobutyryl-CoA: 18.5
Acetyl-CoA: 17.9
3-Methylbutyryl-CoA: 15.5

AMP-binding (3066..3458): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LHYTFDVHVQETFMVAGGEFNEYGPVEASIVTTI
Nearest Stachelhaus code(s):: Trp or Pro DVYFVGGICK (53% 8Å match); Trp DVAVVGEVVK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A

NZ_SLWR01000002.1 - Region 1 - lanthipeptide-class-iv

Download region GenBank file

Download region SVG

Location: 355,884 - 378,541 nt. (total: 22,658 nt) Show pHMM detection rules used

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

EV646_RS07670: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase dehydratase

EV646_RS07685: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

EV646_RS07700: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002699.2	0.37	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0001151.5	0.35	other (non-nrp beta-lactam)	valclavam, (-)-2-(2-hydroxyethyl)clavam	Streptomyces antibioticus
BGC0000046.4	0.35	PKS	depudecin	Alternaria brassicicola
BGC0002180.2	0.34	PKS	(+)-asperlin, (2Z,4Z,6E)-octa-2,4,6-trienoic acid	Aspergillus nidulans FGSC A4
BGC0001959.4	0.34	other (other)	taylorflavin A, taylorflavin B	Streptomyces hiroshimensis
BGC0000678.5	0.33	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002641.2	0.32	NRPS	bacillothiazol A, bacillothiazol B, bacillothiazol C, bacillothiazol D, bacillothiazol E, bacillothiazol F, bacillothiazol G, bacillothiazol H, bacillothiazol I, bacillothiazol J, bacillothiazol K, bacillothiazol L, bacillothiazol M, bacillothiazol N	Bacillus velezensis FZB42
BGC0001582.4	0.32	NRPS	ecumicin	Nonomuraea sp. MJM5123
BGC0001521.3	0.32	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0001366.5	0.31	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002699.2	0.37	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0001151.5	0.35	other (non-nrp beta-lactam)	valclavam, (-)-2-(2-hydroxyethyl)clavam	Streptomyces antibioticus
BGC0000046.4	0.35	PKS	depudecin	Alternaria brassicicola
BGC0002180.2	0.34	PKS	(+)-asperlin, (2Z,4Z,6E)-octa-2,4,6-trienoic acid	Aspergillus nidulans FGSC A4
BGC0001959.4	0.34	other (other)	taylorflavin A, taylorflavin B	Streptomyces hiroshimensis
BGC0000678.5	0.33	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002641.2	0.32	NRPS	bacillothiazol A, bacillothiazol B, bacillothiazol C, bacillothiazol D, bacillothiazol E, bacillothiazol F, bacillothiazol G, bacillothiazol H, bacillothiazol I, bacillothiazol J, bacillothiazol K, bacillothiazol L, bacillothiazol M, bacillothiazol N	Bacillus velezensis FZB42
BGC0001582.4	0.32	NRPS	ecumicin	Nonomuraea sp. MJM5123
BGC0001521.3	0.32	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0001366.5	0.31	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

Additional weak hits (4):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.62 out of 18.57

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.41 out of 26.33

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.33 out of 25.75

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_SLWR01000002.1 - Region 2 - lanthipeptide-class-iii

Download region GenBank file

Download region SVG

Location: 438,995 - 461,535 nt. (total: 22,541 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000513.3	0.65	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000496.5	0.63	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.61	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.58	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.57	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.54	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000518.3	0.48	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000546.3	0.46	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0001210.5	0.43	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002064.2	0.41	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000513.3	0.65	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000496.5	0.63	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.61	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.58	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.57	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.54	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000518.3	0.48	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000546.3	0.46	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0001210.5	0.43	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002064.2	0.41	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EV646_RS08065

EV646_RS08070

Leader:

MALLDLQGLEAPGGHGGGGGG

Core:

GSTLTVLTCGSQRPSNLSVVLCH

Core with dehydrations:

GDhaDhbLDhbVLDhbCysGDhaQRPDhaNLDhaVVLCysH

antiSMASH-DB 4.0 matches for EV646_RS08070

100.0%

NZ_SLWR01000002.1:

Lanthipeptides: EV646_RS08070_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVLTCGSQRPSNLSVVLCH

1

GSTLTVLTCGSQRPSNLSVVLCH

23

80.0%

NC_013729.1and 1 other:

NZ_CP114065.1 Lanthipeptides: OX958_RS04450_lanthipeptide

Lanthipeptides: KFLA_RS37165_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVL CGSQRPSNLS++LCH

3

GSTLTVLGCGSQRPSNLSLLLCH

25

79.2%

NZ_JACHIN010000017.1:

Lanthipeptides: HNR40_RS44190_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVLTC S +PSNLSV LCH

2

GSTLTVLTCHSGKPSNLSVALCH

24

with 68 more sequence matches

Only the first 10 are displayed here.

78.3%

NZ_JACHJP010000003.1and 2 others:

NZ_JACHJP010000003.1 Lanthipeptides: FHS44_RS18405_lanthipeptideNZ_JACHJP010000003.1 Lanthipeptides: FHS44_RS18400_lanthipeptide

Lanthipeptides: FHS44_RS18410_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVL C S PSNLS+VLCH

1

GSTLTVLGCESHTPSNLSLVLCH

23

78.3%

NZ_KI911782.1:

Lanthipeptides: JIAGA_RS34345_lanthipeptide

3

TLTVLTCGSQRPSNLSVVLCH

23

TLTVLTCGS +PSNLSV LCH

1

TLTVLTCGSVKPSNLSVALCH

21

75.0%

NZ_CP017717.1and 2 others:

NZ_FNDJ01000002.1 Lanthipeptides: BLS95_RS05705_lanthipeptideNZ_FNVT01000002.1 Lanthipeptides: C4V41_RS07345_lanthipeptide

Lanthipeptides: BKM31_RS59280_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVL+C S +PSNLSV LCH

2

GSTLTVLSCHSGKPSNLSVALCH

24

75.0%

NZ_CP084058.1:

Lanthipeptides: LCN96_RS39235_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVL+C S PSNLSV LCH

2

GSTLTVLSCHSGAPSNLSVALCH

24

75.0%

NZ_JACDUR010000006.1and 2 others:

NZ_JPMW01000001.1 Lanthipeptides: EV45_RS41010_lanthipeptideNZ_JPMW01000001.1 Lanthipeptides: EV45_RS41005_lanthipeptide

Lanthipeptides: HNR30_RS31115_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLT LTC S +PSNLSV LCH

2

GSTLTALTCNSGKPSNLSVALCH

24

75.0%

NZ_JAHFZZ010000011.1:

Lanthipeptides: KLK06_RS32640_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLT LTC S PSNLSV LCH

2

GSTLTALTCQSGHPSNLSVALCH

24

75.0%

NZ_JAIWLT010000005.1and 1 other:

NZ_JAIWLU010000020.1 Lanthipeptides: LDL05_RS29535_lanthipeptide

Lanthipeptides: LDL08_RS14700_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVL C S +PSNLSV LCH

2

GSTLTVLGCHSGKPSNLSVALCH

24

75.0%

NZ_JAKRKC020000001.1:

Lanthipeptides: MF672_RS25655_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GS+LTV TCGS PSNLSV LCH

2

GSSLTVATCGSHTPSNLSVALCH

24

73.1%

NZ_BBYJ01000032.1and 1 other:

NZ_BBYM01000040.1 Lanthipeptides: AWQ70_RS39425_lanthipeptide

Lanthipeptides: AWQ71_RS42200_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVLTC S RPSNLS+ LCH

4

GSTLTVLTCQSGRPSNLSIALCH

26

73.1%

NZ_SLWN01000005.1:

Lanthipeptides: EV652_RS16625_lanthipeptide

1

GSTLTVLTCGSQRPSNLSVVLCH

23

GSTLTVL C SQRPSNLS++LCH

4

GSTLTVLGCASQRPSNLSLLLCH

26

MIBiG 4.0 matches for EV646_RS08070

47.8%

BGC0000553.3 (SSGG_RS09925):

Lanthipeptide: SRO15-2212

1

GSTLTVLTCGSQRPSNLSVVLC

22

GS +++L C S+LSVVLC

2

GSQVSLLVC---EYSSLSVVLC

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.36 out of 19.26

Confidence: medium

Additional weak hits (3):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.21 out of 26.33

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.09 out of 30.98

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.94 out of 19.52

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

EV646_RS08070 - Class III

Cleavage pHMM score: 10.60

RODEO score: 0

Monoisotopic mass: 2245.2 Da

Molecular weight: 2246.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2264.6 Da N = 1; 2282.6 Da N = 2; 2300.6 Da N = 3; 2318.7 Da N = 4; 2336.7 Da N = 5

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_SLWR01000003.1 - Region 1 - hydrogen-cyanide

Download region GenBank file

Download region SVG

Location: 45,243 - 58,163 nt. (total: 12,921 nt) Show pHMM detection rules used

hydrogen-cyanide: (Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

EV646_RS09685: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

EV646_RS09690: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002345.2	0.50	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001778.5	0.30	other (other)	showdomycin	Streptomyces showdoensis
BGC0002285.2	0.30	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001386.5	0.29	PKS	JBIR-76, JBIR-77	Streptomyces sp. RI-77
BGC0000903.3	0.29	other (other)	fluorometabolite	Streptomyces cattleya
BGC0000897.5	0.29	other (other)	dehydrophos	Streptomyces luridus
BGC0002621.2	0.29	other (other)	angustmycin A, angustmycin B, angustmycin C, aristeromycin, arabinofuranosyladenine	Streptomyces angustmyceticus
BGC0000883.5	0.25	other (other)	arginomycin	Streptomyces arginensis
BGC0000409.5	0.25	NRPS	porothramycin A	Streptomyces albus subsp. albus
BGC0002340.3	0.24	NRPS, other (other)	pyrroloformamide A, pyrroloformamide B, pyrroloformamide D, pyrroloformamide C	Streptomyces sp. CB02980

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002345.2	0.50	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001778.5	0.30	other (other)	showdomycin	Streptomyces showdoensis
BGC0002285.2	0.30	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001386.5	0.29	PKS	JBIR-76, JBIR-77	Streptomyces sp. RI-77
BGC0000903.3	0.29	other (other)	fluorometabolite	Streptomyces cattleya
BGC0000897.5	0.29	other (other)	dehydrophos	Streptomyces luridus
BGC0002621.2	0.29	other (other)	angustmycin A, angustmycin B, angustmycin C, aristeromycin, arabinofuranosyladenine	Streptomyces angustmyceticus
BGC0000883.5	0.25	other (other)	arginomycin	Streptomyces arginensis
BGC0000409.5	0.25	NRPS	porothramycin A	Streptomyces albus subsp. albus
BGC0002340.3	0.24	NRPS, other (other)	pyrroloformamide A, pyrroloformamide B, pyrroloformamide D, pyrroloformamide C	Streptomyces sp. CB02980

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.20 out of 30.22

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_SLWR01000003.1 - Region 2 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 76,629 - 99,343 nt. (total: 22,715 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

EV646_RS09840: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

EV646_RS09845: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

EV646_RS09870: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Hydrolases: deacetylase

EV646_RS09855: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

Lyases: cyclase

EV646_RS09835: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002704.2	0.47	ribosomal	archalan β	Halorussus salinus
BGC0002703.2	0.44	ribosomal	archalan α	Halorussus salinus
BGC0000554.4	0.43	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0001407.5	0.41	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0000500.5	0.41	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0001229.4	0.41	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0001210.5	0.40	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002362.3	0.39	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002704.2	0.47	ribosomal	archalan β	Halorussus salinus
BGC0002703.2	0.44	ribosomal	archalan α	Halorussus salinus
BGC0000554.4	0.43	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0001407.5	0.41	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0000500.5	0.41	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0001229.4	0.41	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0001210.5	0.40	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002362.3	0.39	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EV646_RS09835

allorf_089384_089611

Leader:

MAACASAQLSDRPARPDRPAQSLVVRRYASARGEPHRPALGGVRGA

Core:

QYLHHPDRVCRRSQPCRDRSYRQPHIRWG

Core with dehydrations:

QYLHHPDRVCysRRDhaQPCysRDRDhaYRQPHIRWG

antiSMASH-DB 4.0 matches for allorf_089384_089611

100.0%

NZ_SLWR01000003.1:

Lanthipeptides: allorf_089384_089611_lanthipeptide

1

QYLHHPDRVCRRSQPCRDRSYRQPHIRWG

29

QYLHHPDRVCRRSQPCRDRSYRQPHIRWG

1

QYLHHPDRVCRRSQPCRDRSYRQPHIRWG

29

24.1%

NZ_KQ061217.1:

Lanthipeptides: allorf_2990622_2990774_lanthipeptide

11

RRSQPCRDR

19

RR+ PCRDR

6

RRTHPCRDR

14

20.7%

NZ_JAGGOV010000059.1:

Lanthipeptides: allorf_095707_095805_lanthipeptide

6

PDRVCRRS

13

PDRVC R+

3

PDRVCGRT

10

with 1 more sequence matches

18.6%

NZ_CP049945.1:

Lanthipeptides: allorf_6834514_6834684_lanthipeptide

4

HHPDRVCRRSQPCRDRSY

21

P +VCRR+ P +DR

14

QRPAKVCRRAVPVQDRPL

31

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.97 out of 24.78

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.64 out of 25.88

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.19 out of 29.25

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_089384_089611 - Class II: Cleavage pHMM score: -4.30; RODEO score: 0; Monoisotopic mass: 3665.8 Da; Molecular weight: 3668.1 Da; Number of bridges: 2

TFBS definitions

Regulator	Description

NZ_SLWR01000003.1 - Region 3 - lanthipeptide-class-iii

Download region GenBank file

Download region SVG

Location: 103,791 - 126,358 nt. (total: 22,568 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000496.5	0.67	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000513.3	0.64	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.58	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0002337.2	0.56	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0000563.4	0.52	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.52	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000518.3	0.51	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000496.5	0.67	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000513.3	0.64	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.58	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0002337.2	0.56	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0000563.4	0.52	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.52	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000518.3	0.51	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EV646_RS09950

EV646_RS09945

Leader:

MALLDLQGLETPGYGGGH

Core:

HHPGGSTLTVLGCASQTPSNLSLLLCH

Core with dehydrations:

HHPGGDhaDhbLDhbVLGCysADhaQDhbPDhaNLDhaLLLCysH

antiSMASH-DB 4.0 matches for EV646_RS09945

100.0%

NZ_SLWR01000003.1:

Lanthipeptides: EV646_RS09945_lanthipeptide

1

HHPGGSTLTVLGCASQTPSNLSLLLCH

27

HHPGGSTLTVLGCASQTPSNLSLLLCH

1

HHPGGSTLTVLGCASQTPSNLSLLLCH

27

92.6%

NZ_SOCE01000001.1:

Lanthipeptides: EV138_RS20105_lanthipeptide

1

HHPGGSTLTVLGCASQTPSNLSLLLCH

27

HH GGSTLTVLGCAS TPSNLSLLLCH

1

HHGGGSTLTVLGCASHTPSNLSLLLCH

27

88.9%

NZ_CP043661.1:

Lanthipeptides: F1D05_RS17325_lanthipeptide

2

HPGGSTLTVLGCASQTPSNLSLLLCH

27

H GGSTLTVLGCAS TPSNLSLLLCH

1

HGGGSTLTVLGCASHTPSNLSLLLCH

26

with 71 more sequence matches

Only the first 10 are displayed here.

88.9%

NZ_SHKR01000011.1and 6 others:

NZ_SNWS01000006.1 Lanthipeptides: EV651_RS15130_lanthipeptideNZ_SODF01000002.1 Lanthipeptides: EV650_RS20180_lanthipeptideNZ_SODP01000004.1 Lanthipeptides: EV653_RS35635_lanthipeptideNZ_SODT01000012.1 Lanthipeptides: EV647_RS27100_lanthipeptideNZ_SODU01000003.1 Lanthipeptides: EV137_RS31660_lanthipeptideNZ_SZPZ01000003.1 Lanthipeptides: FDA38_RS24670_lanthipeptide

Lanthipeptides: EV645_RS10765_lanthipeptide

4

GGSTLTVLGCASQTPSNLSLLLCH

27

GGSTLTVLGCASQTPSNLSLLLCH

2

GGSTLTVLGCASQTPSNLSLLLCH

25

88.9%

NZ_SLWN01000005.1:

Lanthipeptides: EV652_RS16625_lanthipeptide

2

HPGGSTLTVLGCASQTPSNLSLLLCH

27

H GGSTLTVLGCASQ PSNLSLLLCH

1

HHGGSTLTVLGCASQRPSNLSLLLCH

26

85.2%

NZ_CP114065.1:

Lanthipeptides: OX958_RS04450_lanthipeptide

2

HPGGSTLTVLGCASQTPSNLSLLLCH

27

H GGSTLTVLGC SQ PSNLSLLLCH

1

HHGGSTLTVLGCGSQRPSNLSLLLCH

26

85.2%

NZ_JACDUR010000003.1and 1 other:

NZ_JACDUR010000003.1 Lanthipeptides: HNR30_RS17930_lanthipeptide

Lanthipeptides: HNR30_RS17930_lanthipeptide

2

HPGGSTLTVLGCASQTPSNLSLLLCH

27

H GGSTLTVLGC S TPSNLSLLLCH

2

HGGGSTLTVLGCNSYTPSNLSLLLCH

27

85.2%

NZ_JACHWA010000004.1:

Lanthipeptides: FHS43_RS13815_lanthipeptide

2

HPGGSTLTVLGCASQTPSNLSLLLCH

27

H GGSTLTVLGCAS PSNLSLLLCH

2

HGGGSTLTVLGCASAKPSNLSLLLCH

27

81.5%

NC_013729.1:

Lanthipeptides: KFLA_RS37165_lanthipeptide

4

GGSTLTVLGCASQTPSNLSLLLCH

27

GGSTLTVLGC SQ PSNLSLLLCH

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

81.5%

NZ_CP029711.1:

Lanthipeptides: DKM19_RS31645_lanthipeptide

4

GGSTLTVLGCASQTPSNLSLLLCH

27

GGSTLTVLGCAS TPSNLSL+LCH

3

GGSTLTVLGCASVTPSNLSLILCH

26

81.5%

NZ_CP029711.1:

Lanthipeptides: DKM19_RS31650_lanthipeptide

4

GGSTLTVLGCASQTPSNLSLLLCH

27

GGSTLTVLGCAS TPSNLSL+LCH

2

GGSTLTVLGCASVTPSNLSLVLCH

25

81.5%

NZ_PVZV01000008.1:

Lanthipeptides: C2865_RS30430_lanthipeptide

4

GGSTLTVLGCASQTPSNLSLLLCH

27

GGSTLTVLGCAS PSNLSLLLCH

2

GGSTLTVLGCASAKPSNLSLLLCH

25

81.5%

NZ_SNWQ01000001.1:

Lanthipeptides: EV643_RS00390_lanthipeptide

2

HPGGSTLTVLGCASQTPSNLSLLLCH

27

H GGSTLTVLGC S PSNLSLLLCH

1

HHGGSTLTVLGCGSHRPSNLSLLLCH

26

MIBiG 4.0 matches for EV646_RS09945

17.6%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

3

PGGSTLTVLGCASQTPS

19

PG T ++GC +T +

9

PGCKTGALMGCNMKTAT

25

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.62 out of 19.52

Confidence: medium

Additional weak hits (4):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.74 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.64 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.40 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.87 out of 20.01

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

EV646_RS09945 - Class III

Cleavage pHMM score: 5.70

RODEO score: 0

Monoisotopic mass: 2616.4 Da

Molecular weight: 2618.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2636.0 Da N = 1; 2654.0 Da N = 2; 2672.0 Da N = 3; 2690.1 Da N = 4; 2708.1 Da N = 5

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NZ_SLWR01000003.1 - Region 4 - arylpolyene,lincosamides

Download region GenBank file

Download region SVG

Location: 548,028 - 594,475 nt. (total: 46,448 nt) Show pHMM detection rules used

Region on contig edge.

lincosamides: (SIS_2 and (GFO_IDH_MocA or GFO_IDH_MocA_C3) and PP-binding)
arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

EV646_RS12125: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

EV646_RS12245: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: PPTase

EV646_RS12305: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: hydrolase

EV646_RS12285: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase synthase

EV646_RS12300: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EV646_RS12320: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

EV646_RS12330: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EV646_RS12335: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

EV646_RS12360: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Translocases: transporter

EV646_RS12265: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.47	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.46	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.46	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.46	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002711.2	0.32	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001736.3	0.31	NRPS	pepteridine A, pepteridine B	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000196.4	0.30	PKS	AQ-256, AQ-270a, AQ-284a	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000225.5	0.30	PKS	frenolicin B	Streptomyces roseofulvus
BGC0000231.5	0.29	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000272.3	0.29	PKS	spore pigment	Streptomyces collinus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.57	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.56	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.56	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.56	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002711.2	0.47	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001736.3	0.46	NRPS	pepteridine A, pepteridine B	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000196.4	0.45	PKS	AQ-256, AQ-270a, AQ-284a	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000225.5	0.45	PKS	frenolicin B	Streptomyces roseofulvus
BGC0000231.5	0.44	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002652.2	0.44	other (other), saccharide	cytosaminomycin F, cytosaminomycin G, streptcytosine P, plicacetin, 40551-F, 12F-plicacetin, streptcytosine D, streptcytosine E	Streptomyces sp. SHP22-7

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

Additional weak hits (5):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.12 out of 27.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.81 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 22.18 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.37 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.23 out of 31.73

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

EV646_RS12300: X

AMP-binding (52..382): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ERYPLGLVGGLGVLTTGGEANMYGMTEVGVIGTD
Nearest Stachelhaus code(s):: nohBza GLGLVGAIIK (53% 8Å match)
Stachelhaus code match:: 60% (weak)

EV646_RS12330: X

AMP-binding (46..423): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AHLLHTATILMQYGFNTGD-----------IVTR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_SLWR01000006.1 - Region 1 - azole-containing-RiPP

Download region GenBank file

Download region SVG

Location: 440,088 - 462,735 nt. (total: 22,648 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

EV646_RS19875: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

EV646_RS19965: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Ligases: synthase

EV646_RS19890: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001485.4	0.39	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0002213.3	0.38	PKS	HEx-pks16 polyketide	Punctularia strigosozonata HHB-11173 SS5
BGC0001146.5	0.38	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0000606.5	0.37	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001587.5	0.37	other (other)	ethylenediaminesuccinic acid hydroxyarginine (EDHA)	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001640.3	0.37	ribosomal	kocurin	Kocuria rosea
BGC0000603.5	0.36	ribosomal	cyclothiazomycin	Streptomyces hygroscopicus
BGC0000943.5	0.36	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0001145.5	0.36	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0001366.5	0.36	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001485.4	0.39	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0002213.3	0.38	PKS	HEx-pks16 polyketide	Punctularia strigosozonata HHB-11173 SS5
BGC0001146.5	0.38	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0000606.5	0.37	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001587.5	0.37	other (other)	ethylenediaminesuccinic acid hydroxyarginine (EDHA)	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001640.3	0.37	ribosomal	kocurin	Kocuria rosea
BGC0000603.5	0.36	ribosomal	cyclothiazomycin	Streptomyces hygroscopicus
BGC0000943.5	0.36	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0001145.5	0.36	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0001366.5	0.36	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.52 out of 26.85

Confidence: medium

Additional weak hits (5):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.39 out of 36.53

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.19 out of 32.74

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.72 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.62 out of 35.63

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

TFBS Finder

Azole RiPPs

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

Core peptide predictions

No core peptides found.

NZ_SLWR01000008.1 - Region 1 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 78,447 - 108,206 nt. (total: 29,760 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

EV646_RS22720: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

EV646_RS22785: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: hydrolase

EV646_RS22840: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase

EV646_RS22775: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001531.5	0.64	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0001478.5	0.64	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.62	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.57	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002464.3	0.55	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0001572.3	0.53	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0001453.5	0.53	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000940.5	0.53	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0002305.2	0.52	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001531.5	0.64	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0001478.5	0.64	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.62	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.57	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002464.3	0.55	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0001572.3	0.53	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0001453.5	0.53	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000940.5	0.53	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0002305.2	0.52	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.86 out of 25.31

Confidence: medium

Additional weak hits (10):

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.26 out of 32.93

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.07 out of 20.97

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.96 out of 26.33

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.85 out of 30.97

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.55 out of 30.25

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 26.40 out of 36.53

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.94 out of 20.01

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.94 out of 29.38

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.90 out of 26.85

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor

NZ_SLWR01000009.1 - Region 1 - T1PKS,hglE-KS

Download region GenBank file

Download region SVG

Location: 127,980 - 179,216 nt. (total: 51,237 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
hglE-KS: (hglE or hglD)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase glycosyltransferase halogenase

EV646_RS25195: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

EV646_RS25285: halogenase

smcogs: SMCOG1119: halogenase

EV646_RS25295: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: hydrolase

EV646_RS25200: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

EV646_RS25255: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: synthase

EV646_RS25210: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

EV646_RS25215: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transporter

EV646_RS25135: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.59	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.59	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.58	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.58	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002154.2	0.57	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001164.3	0.54	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.53	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002192.3	0.51	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.49	PKS	ACT-Toxin II	Alternaria alternata
BGC0000863.3	0.40	other (other)	eicosapentaenoic acid	Pseudoalteromonas sp. DS-12

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002154.2	0.62	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001164.3	0.60	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002192.3	0.59	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.58	PKS	ACT-Toxin II	Alternaria alternata
BGC0000863.3	0.52	other (other)	eicosapentaenoic acid	Pseudoalteromonas sp. DS-12

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 25.67 out of 30.22

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.83 out of 24.78

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 21.07 out of 25.31

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (9):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.83 out of 21.55

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.80 out of 31.73

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.50 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.99 out of 29.65

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.63 out of 25.75

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.24 out of 29.65

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.97 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 127979 - 179216:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Acetyl-CoA)
Non-colinear ordering:: EV646_RS25215 + EV646_RS25210 + EV646_RS25205

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 127979 - 172371:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Acetyl-CoA)
Non-colinear ordering:: EV646_RS25215 + EV646_RS25210 + EV646_RS25205

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 132373 - 179216:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Acetyl-CoA)
Non-colinear ordering:: EV646_RS25215 + EV646_RS25210 + EV646_RS25205

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

EV646_RS25210: Acetyl-CoA

PKS_AT (602..812): Acetyl-CoA

ATSignature: (unknown)

No matches above 50%

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 14.0
inactive: 11.4
Methylmalonyl-CoA: 10.2
Propionyl-CoA: 7.9
Malonyl-CoA: 2.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

EV646_RS25215: mal

PKS_AT (1099..1388): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 76.0
Methylmalonyl-CoA: 34.0
Methoxymalonyl-CoA: 32.7
inactive: 25.0
Propionyl-CoA: 13.3
Isobutyryl-CoA: 12.9
Benzoyl-CoA: 10.8
fatty_acid: 9.6
CHC-CoA: 9.5
2-Methylbutyryl-CoA: 9.3
Ethylmalonyl-CoA: 9.2
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CatR	H2O2-responsive repressor
ClgR	Caseinolytic protease gene regulator
LexA variant 2	Repressor of DNA damage response

NZ_SLWR01000011.1 - Region 1 - NAPAA

Download region GenBank file

Download region SVG

Location: 343,545 - 377,474 nt. (total: 33,930 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EV646_RS30075: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EV646_RS30085: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acetyltransferase glycosyltransferase

EV646_RS29960: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

EV646_RS30065: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Isomerases: mutase

EV646_RS30055: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase

EV646_RS30000: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0002276.2	0.61	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001833.4	0.59	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0002276.2	0.61	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001833.4	0.59	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 31.36 out of 36.53

Confidence: medium

Additional weak hits (4):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.11 out of 25.88

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.16 out of 21.55

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 343544 - 377474:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

EV646_RS30000: X

AMP-binding (19..420): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIFGGEWNTYGPTEATVVACG
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (71% 8Å match); Gln or Tyr DAESVGTVVK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor

NZ_SLWR01000017.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 26,604 - 51,200 nt. (total: 24,597 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EV646_RS35615: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Transferases: sulfhydrylase

EV646_RS35665: O-succinylhomoserine sulfhydrylase

smcogs: SMCOG1168: O-succinylhomoserine sulfhydrylase

Hydrolases: amidase hydrolase

EV646_RS35595: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

EV646_RS35605: amidase

smcogs: SMCOG1105: amidase

EV646_RS35610: isochorismatase

smcogs: SMCOG1027: isochorismatase

EV646_RS35670: amidase

smcogs: SMCOG1105: amidase

Lyases: cyclase

EV646_RS35625: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: synthetase

EV646_RS35635: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002397.2	0.58	terpene	astallatene	Arabidopsis thaliana
BGC0000657.5	0.58	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000658.3	0.56	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002400.2	0.54	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.48	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000678.5	0.44	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001940.3	0.44	PKS	pyxidicycline A, pyxidicycline B	Pyxidicoccus fallax
BGC0001156.5	0.43	terpene	platencin	Streptomyces platensis
BGC0000666.5	0.43	terpene	xiamycin A	Streptomyces sp. SCSIO 02999

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002397.2	0.58	terpene	astallatene	Arabidopsis thaliana
BGC0000657.5	0.58	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000658.3	0.56	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002400.2	0.54	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.48	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000678.5	0.44	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001940.3	0.44	PKS	pyxidicycline A, pyxidicycline B	Pyxidicoccus fallax
BGC0001156.5	0.43	terpene	platencin	Streptomyces platensis
BGC0000666.5	0.43	terpene	xiamycin A	Streptomyces sp. SCSIO 02999

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

EV646_RS35625: T1TS + T1TS

Domain 1 (2 - 329): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (334 - 696): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

EV646_RS35635: PT_FPPS_like

Domain 1 (56 - 336): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.27 out of 26.85

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 24.63 out of 25.88

Confidence: strong

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.42 out of 19.52

Confidence: strong

Additional weak hits (2):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.17 out of 29.38

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
PsrA	Oleic acid responsive regulator
RutR	Regulator of the pyrimidine and purine metabolism

NZ_SLWR01000019.1 - Region 1 - T3PKS

Download region GenBank file

Download region SVG

Location: 16,804 - 57,850 nt. (total: 41,047 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase

EV646_RS37045: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

EV646_RS37055: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EV646_RS37085: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

EV646_RS37105: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

EV646_RS37115: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

EV646_RS37130: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: methyltransferase

EV646_RS37100: Methylation

MITE: MITE0000155Methylation (identity: 68.50%)
smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

Ligases: synthase

EV646_RS37095: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.81	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001831.3	0.53	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001077.4	0.47	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0002362.3	0.45	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0001647.3	0.45	PKS	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0001964.2	0.44	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0000866.5	0.41	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000888.5	0.41	other (other)	bacilysin	Bacillus sp. CS93
BGC0002034.3	0.40	other (other)	perquinoline A, perquinoline B, perquinoline C	Streptomyces sp. IB2014 016-6

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.81	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001831.3	0.53	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001077.4	0.47	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0002362.3	0.45	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0001647.3	0.45	PKS	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0001964.2	0.44	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0000866.5	0.41	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000888.5	0.41	other (other)	bacilysin	Bacillus sp. CS93
BGC0002034.3	0.40	other (other)	perquinoline A, perquinoline B, perquinoline C	Streptomyces sp. IB2014 016-6

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.63 out of 24.78

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.83 out of 20.47

Confidence: medium

Additional weak hits (7):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.53 out of 30.25

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.14 out of 19.26

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.82 out of 25.88

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.67 out of 26.33

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.67 out of 23.43

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.84 out of 29.65

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response
VqsM	Regulator of quorum-sensing signalling systems