LOCUS       NZ_CP028720            11044 bp    DNA     circular CON 21-NOV-2024
DEFINITION  Enterococcus faecalis strain CVM N48037F chromosome, complete
            genome.
ACCESSION   NZ_CP028720
VERSION     NZ_CP028720.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2997828)
  AUTHORS   Tyson,G.H., Sabo,J.L., Hoffmann,M., Hsu,C.H., Mukherjee,S.,
            Hernandez,J., Tillman,G., Wasilenko,J.L., Haro,J., Simmons,M.,
            Wilson Egbe,W., White,P.L., Dessai,U. and Mcdermott,P.F.
  TITLE     Novel linezolid resistance plasmids in Enterococcus from food
            animals in the USA
  JOURNAL   J Antimicrob Chemother 73 (12), 3254-3258 (2018)
   PUBMED   30272180
REFERENCE   2  (bases 1 to 2997828)
  AUTHORS   Tyson,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2018) CVM/OR, FDA, 8401 Muirkirk Rd, MOD2, Laurel,
            MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 603x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004332015.1-RS_2024_11_19
            Annotation Date                   :: 11/19/2024 19:35:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,931
            CDSs (with protein)               :: 2,931
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 30 of 50
            Pseudo Genes (incomplete)         :: 21 of 50
            Pseudo Genes (internal stop)      :: 15 of 50
            Pseudo Genes (multiple problems)  :: 12 of 50
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:04:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1445447
            Orig. end                         :: 1456491
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028720.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6044
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(392..408)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.43"
                     /tool="antismash"
     misc_feature    complement(746..759)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.95"
                     /tool="antismash"
     gene            2278..2478
                     /locus_tag="C7I32_RS07435"
     CDS             2278..2478
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405754.1"
                     /locus_tag="C7I32_RS07435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002365266.1"
                     /transl_table=11
                     /translation="MNVEEVLVSQKTPNAINEETELYGFGYGSVVENITVSKGGGGGGG
                     GGGGCCCCCCCCCGGATGLEE"
     misc_feature    2491..2502
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            2543..3373
                     /locus_tag="C7I32_RS07440"
     CDS             2543..3373
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04424"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379875.1"
                     /locus_tag="C7I32_RS07440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="McbB family protein"
                     /protein_id="WP_002365265.1"
                     /sec_met_domain="TIGR04424 (E-value: 2.6e-88, bitscore:
                     293.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKVNPFIWHDLSEKETFFQTKLSSIIITNKNLVRMLKDLEKNEV
                     INFEESLLSNYFRSNMINNVLVFLEENKLIKKLDIENNKVNEVLVYTDDSEFSELFDYV
                     FSEKFTISHQSSISHLESKNMDGKLLIVFLKAFNFKEYENIVNIAKEKNVVIKIIFSYN
                     HHIYFSNFYKREWYNPCPICFFSELESQLRGEITSDTANFQTIIDLLYTKSQNFAIEYP
                     LQKDDYFTIVYTIMRNLDSDLMKCDLDEVLSIDLNNHLVTKDIAYHWGYCDCYE"
     gene            3366..4022
                     /locus_tag="C7I32_RS07445"
     CDS             3366..4022
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401933.1"
                     /locus_tag="C7I32_RS07445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002401933.1"
                     /transl_table=11
                     /translation="MSDYYWNEYKNVLLDEAYLIDTLKYQQRVVNKQVEENNLDELSDV
                     LGLYNVKYSDIRLAVYPFDELPVFIKTLVSDLQKNYASFIYKVSMPILAITKKIGNVNT
                     GCYSFDFKNNFLILYETDVTKIREIVNDKLVLAFFLNIEDAICLYKKKAYTNGIKEIGY
                     VSKSSQLAISKEKNIIINEMLVPEQQLTNGLGINLRKCLLMEALILEVLTNDIHE"
     gene            4006..4983
                     /locus_tag="C7I32_RS07450"
     CDS             4006..4983
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365263.1"
                     /locus_tag="C7I32_RS07450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_010712829.1"
                     /transl_table=11
                     /translation="MIFTNKSSYGVKSDMFLSNINIGYVNFSRVKIKQGTTAGSAFSVD
                     ENKQLSLIKSYNEYLERLFLGIPLSTNKTISSVNLEKNIVSSRKYSEFGYGNHEFGFND
                     TTGTSSGKLSEVIIQKALTELIEKNEVFCFWYGLKGEKLKQDKEIHDLINDYNFISNMF
                     KIYVVRELSNYSTVIVMGFLEGKLLTSGVCCSITMEQSLILALKEAKGIEWQTFNNPLA
                     KANKFSDKLHKKILKKVKFMDETYKVITKDSIQATKYIETADWINHVEISVIGDDINRG
                     VKTIKCISKQLLNSVPIKINIERQKDKEIIKRYLIDYSIDCPIQ"
     gene            5001..6044
                     /locus_tag="C7I32_RS07455"
     CDS             5001..6044
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401935.1"
                     /locus_tag="C7I32_RS07455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_010711509.1"
                     /sec_met_domain="YcaO (E-value: 1.1e-06, bitscore: 26.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGFLRNKVNFVLNEDLFMSHHYNVYIEKYSKRPIKAYEVDLKNK
                     RQHILSTLGEYLERETLINENSPKEHTVIATNLIDGSIKQVNLDMILFKNSFVDSSGMA
                     SHKKSKPLIWTAYKEFFERQSYITNFLFHTKAQQLNIDKIEEIKKIDRYLTNYLDNVKY
                     YNISLSKNLYVVLAVGYGEKYKAVGLGTSQSARKAVIKSQKEILQYFATSHSKYKEGKV
                     SQGSGLVIDNKDIYHDYFDRLSSKEVKDLYAYLEGSEFIILNNEEKIEKVSHTFTIKNI
                     SNSLNMYPYITMFENNHAKNVKVVKVFDPKWFPNMAPRNFSKEIILNVENILKLKRKNF
                     SELLPFP"
     gene            6057..6737
                     /locus_tag="C7I32_RS07460"
     CDS             6057..6737
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405757.1"
                     /locus_tag="C7I32_RS07460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002365261.1"
                     /transl_table=11
                     /translation="MFISVKSYALFNIKMLLKDKIPLLWSIALPVITFFMNYRSVTNEW
                     ELAYWWVYIVVCSYVYGVGVYALQLKEYGSLKTIFSINNSPWAFFLGNLLTQIMFSFIS
                     VNLLNLIVVILLPLSFLRLFLYSFITIIACLPVAFFSYNLTLIKKVHANTINSIFTMLI
                     FGLFILLNTNIELNKYNPLSILSNVLIEHKNMDVFKYVTFSIFLVFISMYSIVKFEPIS
                     NERR"
     gene            6724..7314
                     /locus_tag="C7I32_RS07465"
     CDS             6724..7314
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394296.1"
                     /locus_tag="C7I32_RS07465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_002394296.1"
                     /transl_table=11
                     /translation="MKEGKAMIEIKDLNIPYVDKKISMNILKEYNLFTGDNGNGKTLLL
                     DYISGVKKAKRTVIKGNESIIYINQNIYFSDRLSGMDFLKFSYGLDGKKSIQSFYQLSD
                     KILKREDIDKLLKKQWGMLSGGEKKFLYALILMSLEREWYILDEPFAFVDKKRKKMLYK
                     IIDIKISEDKGIILTTHEEDEVVLKSVSKVFEI"
     gene            complement(7670..8110)
                     /locus_tag="C7I32_RS07470"
     CDS             complement(7670..8110)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394295.1"
                     /locus_tag="C7I32_RS07470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_002401936.1"
                     /transl_table=11
                     /translation="MTAYSIGEISKMFNIPVSTLRYYDKEGLFPEITRVSGIRKFSNNE
                     IEALRVIECLKKSGLEIKDIKQFMKWTKLGSETFKIRKELFERQKVSVEKELRQMEKVL
                     DMIKFKCWYYDEVLKQGNEVAVKSKIPNNLPKEVKLYYENSH"
     gene            8193..9197
                     /locus_tag="C7I32_RS07475"
     CDS             8193..9197
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365249.1"
                     /locus_tag="C7I32_RS07475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_373465613.1"
                     /sec_met_domain="PF00561 (E-value: 3.1e-05, bitscore: 21.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTIGGKARMLEDTTMIQKWDKVFQKSDKVNHRKVTFTNRFGIAL
                     VADLYEPKRQAEEFPAIAVCGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGI
                     PRNVASGDINTEDFQAAVDFLSVQKNVAQDKIGIIGICGWGGMALNAAAIDTRVKATVV
                     STMYDMSRVTANGYFDSIDVDGRYEMRQQLNAQRIEDFKNGDYARSGGVPDSLSESAPY
                     YIKDYFDYYKTDRGYHKRSLNSNEGWNMTSALSLLNTKFLSYSDEIFSAVLIIHGEKAH
                     SRYFAEDAFKKLQGDNKELLIIPKATHTDLYDKIDIIPFDKMTEFFYQNLEVR"
     gene            9211..9960
                     /locus_tag="C7I32_RS07480"
     CDS             9211..9960
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365247.1"
                     /locus_tag="C7I32_RS07480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_002401938.1"
                     /transl_table=11
                     /translation="MIEKQTAGRDVLGEFAPKFAELNDDVLFGQVWAREDKLSLKMRSI
                     VTITALISKGVIDSSLNYHLETARKNGVTKTEMAELLTHLSFYAGWPNAWAAFRLAKEV
                     YVEDHNKEEHGGLFGQGELNTAYSEYFIGQSYLKPLTDPKETVFIANVTFEPACRNNWH
                     IHHAKSGGGQILICVDGEGWYQEEGKEAQSLKPGDTITIPANVKHWHGAKKDRWFSHLA
                     IEVPGVETSNEWLEPVKNEFYDNLKEC"
     gene            9963..10550
                     /locus_tag="C7I32_RS07485"
     CDS             9963..10550
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156: Chloramphenicol acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401939.1"
                     /locus_tag="C7I32_RS07485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="WP_002401939.1"
                     /transl_table=11
                     /translation="MKVSIEDVINGYRSDAPVDLERDAYAEELYQEAIKIGIEMNTKYH
                     SEEELREIMSYLIGKKVDETFRIFPPFYTDFGKNITLGKNVFINSGTHFQDQGGIVIGD
                     GVFIGHNVVLATINHDLFPKNKRKNHYAPIVLKNNVWIGSNATITSGVTIGEWSVVAAG
                     AVVTKDVPPYTVVGGVPARVLKLIDKEEDGND"
ORIGIN
        1 gcagaaaaaa ttttaggaac tgataataga aaaatagtta gtaatacaac tatagatcca
       61 tataaaaaaa tagcgttttt attaattact tatccgaatg gaaaaaaatt tattggtagc
      121 ggtaatctag tctcttcaga tacagtattg acggcaggtc attgtcttta tgataaggat
      181 atggggggat ttgcaaaatc tattgcggta tatccagggt ataacggcaa ttacgctcct
      241 tttggtgtcg cctattcaaa gaaattaatg acagttagag catgggtgga ttcatctagc
      301 ccacagcatg atatcggtgc tataaaatta gacagaccta ttggaagtaa ggttggttgg
      361 tttggattga caacgaatcc aacatctcca attacactat ctgggtatca tggagattta
      421 aatcgaaaaa tggggacaga aacaggaaat atttcgagac ttacaactaa caatgtttac
      481 tatcaattag attcaacagg tggttctagc ggtagcgggg tatataataa taacaaacaa
      541 attctagcag tccatgctta tggatcaaat tatggaaatt atggtacaag aataaataca
      601 gataagctta atgtggtaaa atcgtggatt actttaaatc cagtagcccc aacaaacact
      661 actggtgtaa attataatag ccatgttgct gaattagggt ggcttgtcaa tgtcgctaat
      721 ggaggtactt caggaactac aggacaaaac agacgtatgg aagcaataaa aattaatatc
      781 aatgattcta aatttgcagg ttcaattcaa tacagaacgc atgttaaaga caaaggatgg
      841 ctgaattggg tttcgaatgg caccgtctca ggaactacag gtcagaagag acaaatggag
      901 gcaattcaga ttaaattaac aggtgagttg tcgagtgctt ataatattca gtatagaaca
      961 catcttcaaa ataagggttg gacaagttgg gtttcgaatg gtgctacctc gggaactaca
     1021 ggacaaaata gacaaatgga agcaattcaa attagattag ttaaaaagta aaaaagctac
     1081 tgtagttatt tagctaattt aaaaaaatga agggttagta tcttgttgta aactaggcac
     1141 aactaagagg tgttaacaac tggaatatta tttttgctgt taacaaaaat aaacatagtg
     1201 ctaagggaaa tatgtttgat gacataaagt taaacttatt taaagtcaga aagtattaaa
     1261 ataagtatct acgggtaatg ctgagaaagt attactcgtt ttttgtttgg cttgaaaatc
     1321 ttaggttgta ccgaggttca ggaaagccct cagagaggta taaaaagtgt ataagcgaca
     1381 agctgagata ggattcttga ctcctacatc aatcaataaa aaataagggt acctagtttc
     1441 gttgcttgtt taaaaagatt aagtagcaga ttagctaaca ttagaaaaaa ataggaagag
     1501 ataaaagaga ttatttaata attagtatgt aaaagtggta taagataaga ggtagcctat
     1561 ttagaaaact tatcggattg tggacggtaa aagagacttt catccgcaga gtaaatttga
     1621 ttagttttaa aggaataaaa agttttttaa tcataagcac tagaaaaacg ccccttattt
     1681 gatgtataat ttgcaaagac attgacctgt gaagatacag gaacagagag gaattaattg
     1741 agtgaagtat acagaaatta tgatttcagg tagtagtttc tgttgaaatg tattgagaat
     1801 actggttttt aatgtctttt ttcgtacagt aaataagttc aaaaagcaat gattgttagc
     1861 tcttgtgtta gcggttgttg ctttttaaat acttaaatat gttttgttat taaacaatat
     1921 gctttcctta tagtaagtat caattcataa aactaggtat tcacaattgt tattaaagag
     1981 gtaaaatccg aaaatatgcg aatatgcatt gtatattata attttaatgt gataaaataa
     2041 atattttaaa aaataatttt tgttagaatt ttcattgata taaaaataga aaaattaatg
     2101 atagttgttt tgttattaat ttttttgttt ttgttgtcta atcaaagact tttagatttt
     2161 tcagtataaa aatacgtttt ttaattagaa taagatgagt ttttgcttga aaaatgattg
     2221 gatagtggta aaataatttc atcaatattg ataaaattat aacggaggtg tattaaaatg
     2281 aatgttgaag aagttttagt gtcgcaaaaa accccaaatg caattaatga agagactgaa
     2341 ttatatgggt ttgggtatgg ttctgtcgtt gagaacatta ctgtctctaa aggtggaggc
     2401 ggtggtggag gcggcggcgg tggctgttgt tgttgttgct gctgttgttg tgggggtgcc
     2461 acaggattag aagaatagaa acttcataag gtaagtggac gcacttacct ctttacatac
     2521 tcaagtaaaa aggagtgaaa aaatgtataa agtaaaccct tttatatggc atgatcttag
     2581 tgaaaaagaa acattctttc aaacaaaact atcttcgata attattacta ataaaaattt
     2641 agttagaatg ttaaaagatt tagaaaaaaa tgaagtaata aattttgagg aatctctatt
     2701 atccaattat tttaggagca acatgattaa taatgtactg gtctttttag aagaaaataa
     2761 attaattaaa aagctagata tagaaaataa taaggttaat gaggttttgg tatatactga
     2821 tgattctgaa ttttctgaat tattcgatta cgtgttttcc gaaaaattta caattagtca
     2881 tcagagcagt atttcccact tagaatctaa aaatatggat ggtaaattgc ttatagtatt
     2941 tttaaaggct ttcaatttta aagaatatga aaatattgta aatatagcca aggaaaagaa
     3001 tgtagtcatt aaaataattt ttagctataa tcatcatatt tatttttcga atttctataa
     3061 aagggaatgg tacaatccat gtccaatatg ttttttttct gaactagaaa gccaattaag
     3121 aggagaaata acttctgata cagctaattt tcagacaatt atagatttac tttatacaaa
     3181 atcacaaaat tttgctatag aatacccgtt gcaaaaggat gattatttta cgatagttta
     3241 taccataatg aggaatttgg atagcgattt aatgaaatgt gacttagatg aagttttatc
     3301 tattgattta aataatcatt tagttacaaa agatattgct tatcattggg ggtattgtga
     3361 ttgctatgag tgattattat tggaatgaat ataagaatgt actattagat gaagcatatt
     3421 taattgatac gttaaaatat caacaaaggg tagtgaataa acaagtagaa gagaataatt
     3481 tagatgaatt gagcgatgtt ttaggtcttt ataatgtgaa atactcggat attaggttag
     3541 cggtatatcc ttttgatgag ttacctgtct ttataaaaac gttagttagt gatttacaaa
     3601 aaaattatgc ttcctttata tataaagtta gtatgccaat actagccata acaaaaaaaa
     3661 tagggaatgt aaatactggt tgttattctt ttgattttaa aaataatttt ttgattctat
     3721 atgaaactga tgtaactaag attagagaaa tagtaaatga taaactggta ttagcttttt
     3781 ttttaaacat agaggatgca atttgtttat ataaaaaaaa ggcgtacaca aatggtataa
     3841 aagaaattgg ttatgtttct aaaagcagtc aattagcgat tagtaaagaa aaaaacataa
     3901 taataaacga aatgctagta ccagagcaac aactgacaaa tggattaggt ataaacttga
     3961 gaaaatgttt gttaatggaa gctttaattt tagaggtgtt aactaatgat attcacgaat
     4021 aaaagttcct acggtgtgaa aagtgatatg tttttatcaa atataaatat tggatatgtg
     4081 aatttttcac gtgtcaagat taagcaagga acgacagcag gtagtgcttt ctcagttgac
     4141 gaaaataaac agttatcact tattaaaagt tataatgaat atttagaaag acttttctta
     4201 gggataccat taagtacaaa taaaactatt tcttctgtaa atttagaaaa aaatattgta
     4261 agtagcagaa aatattcaga atttggatat ggaaatcatg agtttggatt taatgatacg
     4321 acaggaactt catctggaaa actttcagaa gtaataatcc aaaaggcact aacagaattg
     4381 attgagaaaa atgaagtatt ttgtttttgg tatggtttga aaggggaaaa gttaaaacaa
     4441 gataaagaaa ttcatgactt aattaatgat tataatttta tttcaaatat gtttaaaatt
     4501 tatgtagttc gtgaacttag taattattca acagtgatcg tgatggggtt tttagaaggg
     4561 aaattactaa cctcaggtgt atgttgtagt attactatgg aacaatctct aattttggcc
     4621 ttgaaagaag ctaagggaat agagtggcaa acttttaata atcccttagc aaaagcaaat
     4681 aaattttctg ataaattgca taaaaaaatt ttaaaaaaag taaagtttat ggatgaaaca
     4741 tacaaagtta ttacaaaaga ttctatacaa gctacgaaat acattgaaac tgctgattgg
     4801 attaaccatg ttgagatatc cgtaattgga gatgacataa atagaggagt taaaacaata
     4861 aaatgtatat ccaaacagtt actaaattct gtaccaatta agataaacat tgaaagacaa
     4921 aaagataaag agattattaa gagatattta attgattatt ctattgattg tcctatccaa
     4981 tgatagaaag gagaatactt ttgaaaggtt ttttaagaaa taaggtgaat tttgttttaa
     5041 atgaggattt atttatgtca catcactata acgtatatat agaaaaatat tctaaaagac
     5101 ctatcaaagc ctatgaggtt gatttaaaga ataaaaggca gcatattctt tcaactttag
     5161 gagagtattt ggagcgagaa acactcatca acgaaaattc accaaaagag catacggtta
     5221 ttgctacgaa tttaattgat ggaagcatta aacaagttaa tcttgatatg attttgttta
     5281 agaatagttt tgttgattct tctggtatgg cctcgcataa aaaatcaaag ccattaatat
     5341 ggacagcgta taaagaattc tttgaaagac agagctatat tactaacttc ttgtttcata
     5401 ctaaggctca gcaattaaat atagataaga tagaagaaat aaaaaaaata gacagatatt
     5461 tgactaatta tttagacaat gtcaaatatt ataatatatc tttaagtaag aacctttacg
     5521 tggtacttgc cgttgggtat ggtgaaaaat acaaagcagt gggtttggga actagtcaat
     5581 cagcaagaaa agcagtaata aaatcacaaa aagaaatatt acaatatttt gctacatcgc
     5641 attcaaaata taaagaagga aaagttagtc aaggctctgg attagtaatt gacaataagg
     5701 acatttatca tgactatttt gatagattga gctcgaaaga agttaaagat ttatatgcat
     5761 atcttgaagg tagtgaattt attattctga ataatgaaga gaaaatagaa aaagttagtc
     5821 atacatttac tatcaaaaat ataagtaatt ctcttaatat gtatccgtat attacaatgt
     5881 ttgaaaataa tcatgctaag aatgtgaaag tagtgaaggt atttgaccca aagtggttcc
     5941 ccaacatggc accaagaaat ttttcgaaag aaataatatt aaatgtagag aatatattaa
     6001 aattaaaaag aaaaaatttt tcagaactgt taccatttcc gtaaggagga gtgaaaatgt
     6061 ttatctcagt taaatcatat gcattgttta atattaaaat gttactgaaa gataaaattc
     6121 ctttattgtg gtcaatagcc ttacctgtaa ttactttttt tatgaattat agaagtgtaa
     6181 caaatgaatg ggaattggca tattggtggg tatatattgt tgtttgttcc tatgtttatg
     6241 gagtaggagt ttacgcatta caattaaagg aatatggaag cttaaaaaca attttttcaa
     6301 taaataattc accttgggca ttttttttag gcaatctatt gacgcaaatt atgtttagct
     6361 ttatcagtgt aaacttactt aatttaattg tggttatttt actaccatta tcgtttctcc
     6421 gtcttttttt atatagcttt attactatta tagcgtgttt accagttgca ttttttagtt
     6481 acaatttaac tctaataaaa aaagtgcatg ctaatactat aaattcaata tttacaatgc
     6541 taatttttgg tttatttata ttgttaaata cgaatataga gttaaataaa tataaccctc
     6601 tgtcaatttt atctaatgtt ttaattgaac ataaaaatat ggatgtcttc aaatatgtta
     6661 ctttttcaat atttttagtt tttattagta tgtattctat agttaaattt gaaccgattt
     6721 caaatgaaag aaggtaaagc tatgatcgaa ataaaggatt taaatattcc ttatgtagat
     6781 aaaaaaataa gtatgaatat tttaaaagag tataatttat ttactggtga taatggtaac
     6841 ggaaaaacct tactattaga ttacatttca ggagtaaaaa aggcaaaaag aactgtaatt
     6901 aaggggaatg aaagtatcat atatattaat caaaacatat atttttctga tcgtttatca
     6961 ggaatggact ttctgaaatt tagttatggt ttggacggaa aaaaaagtat tcaatctttt
     7021 tatcaattaa gcgataaaat tcttaagcgt gaagatattg ataagttgct taaaaaacaa
     7081 tggggaatgc tttcaggagg ggaaaaaaag ttcctttatg ctcttatttt gatgtcttta
     7141 gaaagagaat ggtatatttt agatgaaccg tttgcttttg tagataagaa aagaaaaaaa
     7201 atgttatata agattataga tattaagata tcagaagata agggtattat tttaactact
     7261 catgaagaag atgaagtagt attaaaatca gttagcaagg tttttgaaat ataactgtaa
     7321 gaagtagtgt catcatttta gaatatttaa tgttgtacga taaaagaatg aattataata
     7381 agctcagact gtagtttaaa ttatagtaaa tataaaataa ttatgttttt gtatagtagt
     7441 ttttttggaa agtatagata atactggttt ttaatgtttt tgtttgtata gtaaataagt
     7501 tcaaaaagca acgattgtta gctctgctgc tagcagttgt tgctttttga acttatttaa
     7561 atagtgtagt ctatgaaaat taaagtgcta gtgacaaatc tactctacta atttaaataa
     7621 cgtttgtttt ttgtttagat taactcatta aatgtacaat cgtttcaaat taatgagagt
     7681 tttcgtaata aagttttacc tcttttggta agttatttgg aatttttgat ttaacagcca
     7741 cctcgttccc ttgcttaagg acttcatcgt agtaccagca tttaaattta atcatatcaa
     7801 gaactttttc catttgtcgc aattcttttt ctacgcttac tttttgtcgt tcaaatagtt
     7861 cttttcgaat tttgaaagtt tctgacccta attttgtcca tttcataaat tgtttaatat
     7921 ctttaatttc taatccagat ttcttcaaac actcaatcac tcttaaagct tcaatttcgt
     7981 tattagaaaa ttttcgaata ccggaaacac gcgttatttc aggaaataat ccttctttat
     8041 cataatagcg gagagttgaa actggaatgt taaacatttt tgatatttcg ccgattgaat
     8101 atgctgtcat agctcctcct gaaaaatttt tataatcaga cttgacctaa agttaagttt
     8161 aggtagtaag attgtattat taaaaaaatt aaatgtcaac tataggaggg aaagcaagga
     8221 tgctagaaga tactacaatg attcaaaaat gggataaggt attccaaaaa agcgataagg
     8281 ttaatcatcg taaagtgact tttactaatc gatttggaat agctctagtt gctgatttat
     8341 atgaaccaaa aagacaagcg gaagagtttc ctgcaattgc tgtgtgcggt ccgttcgggg
     8401 cagttaaaga acaatcatca ggactttatg ctcaaacaat ggcagaacgg ggctttttga
     8461 cgatagcttt tgatccgtca tttactggag aaagtggagg gatcccacgt aatgttgctt
     8521 ctggcgatat taacactgag gatttccaag cggctgttga tttcttgtcg gtccaaaaaa
     8581 atgttgctca ggataaaatt ggaattattg gtatttgcgg ttggggcggc atggcactaa
     8641 atgcagctgc aattgataca agagtaaaag caaccgttgt ttcgactatg tatgatatgt
     8701 ctcgtgtaac agctaatggt tattttgatt ctattgatgt agatggtcga tatgaaatgc
     8761 gtcaacaact caatgcacaa cgtattgaag atttcaaaaa tggggattat gctcgttcgg
     8821 gtggtgttcc agatagtttg tcagaaagtg cgccttatta tattaaagat tatttcgatt
     8881 attacaaaac agaccgcggc tatcataagc gatcgttgaa ctcaaatgaa ggttggaata
     8941 tgacgtctgc tctttcattg ttaaatacta aatttctgtc ttatagtgat gagattttta
     9001 gtgcggtgtt aattattcat ggtgaaaaag ctcattctcg atatttcgca gaagacgctt
     9061 ttaagaagtt gcaaggagat aacaaggagt tgctaattat tcctaaagca acacacactg
     9121 atctctacga caaaatagac atcattccat ttgataaaat gaccgaattt ttttatcaaa
     9181 atttggaagt aagataggag gaatttgtaa atgattgaga aacaaactgc tggaagagat
     9241 gttttaggtg aatttgcacc caaatttgct gagctgaatg atgatgtgtt gtttggtcaa
     9301 gtatgggcaa gagaagataa actttctttg aaaatgcgtt ctattgtgac aataacggcg
     9361 ctcatttcaa agggagttat cgattcatct ttgaactatc atctggaaac tgctcggaaa
     9421 aatggtgtaa ctaagacaga aatggctgaa ttattaacac atctttcatt ttatgcagga
     9481 tggccgaatg cttgggcagc gtttcgattg gcgaaagaag tgtatgtgga ggaccataac
     9541 aaagaagaac atgggggatt gtttggtcaa ggggaattaa acacagctta ttcagaatac
     9601 tttattggtc aaagttattt gaaaccattg actgatccaa aagaaacagt gtttattgcc
     9661 aatgtcacgt ttgaaccagc ttgccgtaat aattggcata ttcatcatgc gaaatcagga
     9721 ggaggccaaa tcttgatatg cgtagatggt gaaggttggt atcaagaaga aggaaaagaa
     9781 gcacaaagcc tgaagccagg ggataccatt acaatacctg ccaatgtaaa gcattggcac
     9841 ggtgcaaaga aagatcggtg gtttagccac ttagcgattg aagtgcctgg ggtagaaaca
     9901 tcaaacgaat ggctagagcc tgttaaaaat gaattttatg ataatctaaa ggagtgttaa
     9961 taatgaaagt aagtattgaa gatgttatta atgggtacag aagtgatgct cctgttgatt
    10021 tagagcgaga tgcgtatgct gaggagcttt atcaagaagc gataaaaatt ggaatagaaa
    10081 tgaataccaa gtatcattca gaagaggaat tacgagaaat tatgagttac ttgattggaa
    10141 aaaaagtaga tgaaactttt cgtatatttc caccttttta tacggacttt ggtaaaaaca
    10201 ttacacttgg caaaaatgtc tttataaact cagggacgca tttccaagac caaggtggga
    10261 ttgtgattgg tgatggtgta tttataggac ataatgtagt acttgctaca attaatcacg
    10321 acctatttcc aaagaataaa cgtaaaaatc attatgcgcc aattgtgtta aaaaataacg
    10381 tatggattgg ttcaaatgct acgattactt caggtgtaac gattggtgag tggtcagtag
    10441 ttgcagcagg tgcggtcgtc acaaaagatg ttccacccta cactgtagta ggtggcgttc
    10501 ccgctcgtgt gctaaaatta atagataagg aagaggatgg taatgattga taaaacaaca
    10561 aagtcaaaag cagatgagac tgataatatg aagaaacttt atcttatgcg acatggtgaa
    10621 acgctattta ataaattagg gaaaattcaa ggagcaagtg actcaccttt gactgaagta
    10681 ggaataaaac aagcaagaat tgcaaaagag tacttccaaa atcgtaatat taaattagat
    10741 gcttattatt cctctacaca agagcgtgca tcagatacac tagagataat tataggaaat
    10801 aaaaaatata agcgaatgaa ggaactaaaa gaatggaatt ttggtatatt tgaaggagaa
    10861 agagaatatt taaaccctaa aagacctatt ggggaaactt catatggtga ttcttttgtg
    10921 ccttatggcg gagagtcttc aaaagtggtg caggaaagaa taaacgaagg acttactaaa
    10981 attatggaac aagaaaacaa tcagaacgtt ttggcagtta gccatggggg agctatgtat
    11041 ctat
//
