GCF_004323735.1 - 14 region(s)

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Location: 419,298 - 441,553 nt. (total: 22,256 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

EKD16_RS01975: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	terpene	Similarity score	Type	Compound(s)	Organism
BGC0001181.4		0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001746.3		0.75	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0001181.4		0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001746.3		0.75	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

EKD16_RS01975: T1TS + T1TS

Domain 1 (2 - 329): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (331 - 707): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (7):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.09 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.87 out of 29.65

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.72 out of 26.85

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.42 out of 36.53

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.78 out of 29.65

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.10 out of 20.01

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.94 out of 24.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_CP036455.1 - Region 2 - ectoine

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Location: 564,648 - 575,064 nt. (total: 10,417 nt) Show pHMM detection rules used

ectoine: ectoine_synt

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

EKD16_RS02550: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000853.3	0.81	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.81	other (ectoine)	ectoine	Streptomyces sp.
BGC0000852.5	0.70	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.66	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.55	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.55	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.55	other (other)	ectoine	Methylomicrobium kenyense
BGC0000854.3	0.54	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0000857.3	0.51	other (other)	ectoine	Methylophaga alcalica
BGC0000860.3	0.50	other (other)	ectoine	Methylarcula marina

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000853.3	0.81	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.81	other (ectoine)	ectoine	Streptomyces sp.
BGC0000852.5	0.70	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.66	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.55	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.55	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.55	other (other)	ectoine	Methylomicrobium kenyense
BGC0000854.3	0.54	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0000857.3	0.51	other (other)	ectoine	Methylophaga alcalica
BGC0000860.3	0.50	other (other)	ectoine	Methylarcula marina

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.78 out of 29.72

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.86 out of 18.57

Confidence: medium

Additional weak hits (7):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.53 out of 27.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.99 out of 21.55

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.17 out of 32.74

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.82 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.82 out of 30.25

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.00 out of 32.38

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
NtrC	Nitrogen regulatory protein C
ToxT	Regulator of MSHA biosynthesis

NZ_CP036455.1 - Region 3 - thioamide-NRP

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Location: 879,527 - 929,094 nt. (total: 49,568 nt) Show pHMM detection rules used

thioamide-NRP: (ATP-grasp and AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKD16_RS03915: dehydrogenase

smcogs: SMCOG1072: dehydrogenase

EKD16_RS03930: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

EKD16_RS03935: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

EKD16_RS03975: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Hydrolases: hydrolase

EKD16_RS03850: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

EKD16_RS03855: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

EKD16_RS03895: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

EKD16_RS04010: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: decarboxylase

EKD16_RS03955: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase synthase

EKD16_RS03925: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EKD16_RS03945: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

EKD16_RS03985: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002499.2	0.52	NRPS, PKS	enteromycin	Streptomyces achromogenes subsp. streptozoticus
BGC0000917.3	0.49	other (other)	molybdenum cofactor	Rhodobacter capsulatus
BGC0001137.5	0.48	other (other)	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0002405.2	0.48	saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0000585.5	0.47	ribosomal	microcin C7	Escherichia coli
BGC0001162.3	0.47	PKS	1-heptadecene	Moorea producens JHB
BGC0001161.5	0.47	PKS	1-heptadecene	Moorea bouillonii PNG
BGC0001163.3	0.47	PKS	1-heptadecene	Moorea producens 3L

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002499.2	0.52	NRPS, PKS	enteromycin	Streptomyces achromogenes subsp. streptozoticus
BGC0000917.3	0.49	other (other)	molybdenum cofactor	Rhodobacter capsulatus
BGC0001137.5	0.48	other (other)	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0002405.2	0.48	saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0000585.5	0.47	ribosomal	microcin C7	Escherichia coli
BGC0001162.3	0.47	PKS	1-heptadecene	Moorea producens JHB
BGC0001161.5	0.47	PKS	1-heptadecene	Moorea bouillonii PNG
BGC0001163.3	0.47	PKS	1-heptadecene	Moorea producens 3L

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 30.11 out of 30.11

Confidence: strong

Additional weak hits (12):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.85 out of 21.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.01 out of 20.97

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.05 out of 27.55

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.54 out of 24.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.88 out of 20.01

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.76 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.90 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.24 out of 26.12

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.03 out of 26.06

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.87 out of 20.01

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

EKD16_RS03925: NH2

CAL_domain (38..472): NH2

minowa: NH2

Prediction, score:

NH2: 140.3
Acetyl-CoA: 60.1
fatty_acid: 32.1
AHBA: 14.3
shikimic_acid: 0.0

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CelR	Cellobiose uptake repressor

NZ_CP036455.1 - Region 4 - azole-containing-RiPP,lanthipeptide-class-ii

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Location: 1,419,328 - 1,474,736 nt. (total: 55,409 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

EKD16_RS06125: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: glycosyltransferase methyltransferase

EKD16_RS06120: methyltransferase

smcogs: SMCOG1089: methyltransferase

EKD16_RS06175: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

EKD16_RS06190: methyltransferase

smcogs: SMCOG1248: methyltransferase

Lyases: cyclase dehydratase

EKD16_RS06085: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

EKD16_RS06195: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001753.3	0.55	ribosomal	radamycin, globimycin	Streptomyces globisporus subsp. globisporus
BGC0000528.5	0.35	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002311.3	0.34	ribosomal	cutimycin	Cutibacterium acnes KPA171202
BGC0001640.3	0.26	ribosomal	kocurin	Kocuria rosea
BGC0001472.5	0.25	ribosomal	berninamycin A	Streptomyces bernensis
BGC0000548.4	0.24	ribosomal	salivaricin A	Streptococcus salivarius
BGC0002825.2	0.23	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0001789.3	0.22	ribosomal	suicin 90-1330	Streptococcus suis
BGC0002115.3	0.22	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0002116.3	0.22	ribosomal	darobactin A	Photorhabdus khanii HGB 1456

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001753.3	0.81	ribosomal	radamycin, globimycin	Streptomyces globisporus subsp. globisporus
BGC0002311.3	0.67	ribosomal	cutimycin	Cutibacterium acnes KPA171202
BGC0000528.5	0.61	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001472.5	0.59	ribosomal	berninamycin A	Streptomyces bernensis
BGC0002363.3	0.54	ribosomal	berninamycin K, berninamycin J, berninamycin A, berninamycin B	Streptomyces sp.
BGC0001201.3	0.52	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0002316.2	0.51	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001145.5	0.51	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0001707.5	0.50	ribosomal	nosiheptide	Streptomyces sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EKD16_RS06195

EKD16_RS25235

Leader:

MSTALLERTETEIDPAGATADDLHVDNLISESHDIYAA

Core:

SSGWVCTLTIECGTLVCACQ

Core with dehydrations:

DhaDhaGWVCysDhbLDhbIECysGDhbLVCysACysQ

antiSMASH-DB 4.0 matches for EKD16_RS25235

100.0%

BGC0002110.3 (QKV49893.1):

Lanthipeptides: EKD16_RS25235_lanthipeptide

1

SSGWVCTLTIECGTLVCACQ

20

SSGWVCTLTIECGTLVCACQ

1

SSGWVCTLTIECGTLVCACQ

20

95.0%

NZ_JAODUN010000003.1:

Lanthipeptides: BXY51_RS10560_lanthipeptide

1

SSGWVCTLTIECGTLVCAC

19

SSGWVCTLTIECGTLVCAC

1

SSGWVCTLTIECGTLVCAC

19

95.0%

NZ_KB895893.1and 1 other:

NZ_KB900614.1 Lanthipeptides: SALPAC6_RS0114155_lanthipeptide

Lanthipeptides: C569_RS0129325_lanthipeptide

1

SSGWVCTLTIECGTLVCACQ

20

SSGWVCTLTIECGT+VCACQ

1

SSGWVCTLTIECGTVVCACQ

20

with 104 more sequence matches

Only the first 10 are displayed here.

90.0%

NZ_JAGPXN010000019.1:

Lanthipeptides: KBX06_RS22045_lanthipeptide

1

SSGWVCTLTIECGTLVCACQ

20

SSGWVCTLTIECGT+VCAC+

1

SSGWVCTLTIECGTVVCACR

20

81.0%

NZ_CP015515.1and 1 other:

NZ_PSUO01000011.1 Lanthipeptides: C5C18_RS08595_lanthipeptide

Lanthipeptides: A6122_RS12855_lanthipeptide

1

SSGWVCTLTIECGTLVCACQ

20

SSGW+CTLTIECGT+VCAC+

2

SSGWMCTLTIECGTVVCACR

21

76.2%

NC_009480.1and 10 others:

NZ_CP033724.1 Lanthipeptides: EFE39_RS10220_lanthipeptideNZ_CP047051.1 Lanthipeptides: GRD74_RS10365_lanthipeptideNZ_CP047054.1 Lanthipeptides: GRD61_RS10460_lanthipeptideNZ_CP053860.1 Lanthipeptides: DQP62_RS10825_lanthipeptideNZ_CP076352.1 Lanthipeptides: KN218_RS09900_lanthipeptideNZ_CP085137.1 Lanthipeptides: LHJ48_RS10290_lanthipeptideNZ_CP085140.1 Lanthipeptides: LHJ47_RS10305_lanthipeptideNZ_CP095274.1 Lanthipeptides: MU580_RS10460_lanthipeptideNZ_PSTW01000024.1 Lanthipeptides: C5C03_RS09675_lanthipeptideNZ_SISU01000002.1 Lanthipeptides: EOG37_RS09890_lanthipeptide

Lanthipeptides: CMM_RS10575_lanthipeptide

1

SSGWVCTLTIECGTLVCACQ

20

SSGW+CTLTIECGT++CAC+

2

SSGWLCTLTIECGTIICACR

21

76.2%

NZ_AUBN01000022.1:

Lanthipeptides: G448_RS16025_lanthipeptide

1

SSGWVCTLTIECGTLVCACQ

20

SSGW CTLTIECGT +CAC+

2

SSGWFCTLTIECGTFICACR

21

76.2%

NZ_CP028130.1:

Lanthipeptides: C7V51_RS14690_lanthipeptide

1

SSGWVCTLTIECGTLVCACQ

20

SSGW+CTLTIECGT++CAC+

2

SSGWMCTLTIECGTIICACR

21

76.2%

NZ_CP061274.1and 1 other:

NZ_JADKRR010000001.1 Lanthipeptides: ITJ40_RS10085_lanthipeptide

Lanthipeptides: H9X71_RS09850_lanthipeptide

1

SSGWVCTLTIECGTLVCACQ

20

SSGW+CTLTIECGT +CAC+

2

SSGWLCTLTIECGTFICACR

21

45.5%

NZ_CP034669.1:

Lanthipeptides: EJ065_RS32040_lanthipeptide

1

SSGWVCTLTIECG

13

S G+VCTLT ECG

9

SCGYVCTLTTECG

21

40.0%

NZ_CP022413.2and 1 other:

NZ_JAQEYW010000009.1 Lanthipeptides: PG422_RS09400_lanthipeptide

Lanthipeptides: CGC63_RS01420_lanthipeptide

2

SGWVCTLTIECGTL

15

SG VCT+++ECG++

1

SGVVCTISVECGSI

14

40.0%

NZ_JALMDK010000017.1:

Lanthipeptides: allorf_059288_059443_lanthipeptide

1

SSGWVCTLTIEC

12

S G++CTLT EC

2

SGGYICTLTWEC

13

40.0%

NZ_LWKZ01000001.1:

Lanthipeptides: A6A06_RS41385_lanthipeptide

1

SSGWVCTLTIEC

12

SSG+ CT+T EC

2

SSGYFCTITTEC

13

MIBiG 4.0 matches for EKD16_RS25235

26.7%

Lanthipeptide: birimositide

1

SSGWVCTLTIEC

12

+ GW CTLT EC

15

NGGW-CTLTKEC

25

25.0%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

1

SSGWVCTLTIEC

12

+ G CTLT+EC

12

NKGAYCTLTVEC

23

12.8%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

6

CTLTIEC

12

CTL+ EC

28

CTLSKEC

34

allorf_1457494_1457619

Leader:

MLVCRAAVGLRKGGSGGG

Core:

WGSDGRPRARCLGSYLWVLYLCS

Core with dehydrations:

WGDhaDGRPRARCysLGDhaYLWVLYLCysDha

antiSMASH-DB 4.0 matches for allorf_1457494_1457619

100.0%

Lanthipeptides: allorf_1457494_1457619_lanthipeptide

1

WGSDGRPRARCLGSYLWVLYLCS

23

WGSDGRPRARCLGSYLWVLYLCS

1

WGSDGRPRARCLGSYLWVLYLCS

23

20.0%

NZ_JADKRR010000001.1:

Lanthipeptides: allorf_2147275_2147397_lanthipeptide

6

RPRARCLG

13

RPR+RC+

1

RPRSRCIS

8

14.3%

NC_015434.1:

Lanthipeptides: VAB18032_RS15325_lanthipeptide

11

CLGSYLWV

18

CLG++ WV

22

CLGTFCWV

29

allorf_1457522_1457644

Leader:

MPGGTAAPIMSRGTKPTSRTRDNGHAGA

Core:

RRTPTRRRCRLS

Core with dehydrations:

RRDhbPDhbRRRCysRLDha

antiSMASH-DB 4.0 matches for allorf_1457522_1457644

100.0%

Lanthipeptides: allorf_1457522_1457644_lanthipeptide

1

RRTPTRRRCRLS

12

RRTPTRRRCRLS

1

RRTPTRRRCRLS

12

21.1%

NZ_CP029711.1and 1 other:

NZ_CP029711.1 Lanthipeptides: allorf_06103854_06104042_lanthipeptide

Lanthipeptides: allorf_06103854_06104042_lanthipeptide

3

TPTRRRCRLS

12

PTRRR RLS

14

NPTRRRERLS

23

allorf_1460897_1461118

Leader:

MLVHSSAARLFRMFLRGGVTCQGA

Core:

ASGHIGTRRTYDLGGRCYFAGQRLSRPARRRRSGEFDGGSTLTGSDVPT

Core with dehydrations:

ADhaGHIGDhbRRDhbYDLGGRCysYFAGQRLDhaRPARRRRDhaGEFDGGDhaDhbLDhbGDhaDVPDhb

antiSMASH-DB 4.0 matches for allorf_1460897_1461118

100.0%

Lanthipeptides: allorf_1460897_1461118_lanthipeptide

1

ASGHIGTRRTYDLGGRCYFAGQRLSRPARRRRSGEFDGGSTLTGSDVPT

49

ASGHIGTRRTYDLGGRCYFAGQRLSRPARRRRSGEFDGGSTLTGSDVPT

1

ASGHIGTRRTYDLGGRCYFAGQRLSRPARRRRSGEFDGGSTLTGSDVPT

49

14.3%

NC_017943.1:

Lanthipeptides: allorf_132319_132471_lanthipeptide

16

RCYFAGQRLSRPAR

29

RC+ Q LSR R

10

RCFLVIQLLSRSLR

23

14.3%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: allorf_141750_141899_lanthipeptide

Lanthipeptides: allorf_141750_141899_lanthipeptide

14

GGRCYFAGQRLSRPARRRRSGEF

36

GG C++ G + P R +G+

6

GGSCWWPGGSYNLPLRLSVAGDI

28

with 1 more sequence matches

14.3%

NZ_JANVAG010000003.1:

Lanthipeptides: allorf_433065_433280_lanthipeptide

16

RCYFAGQRLSRPARR

30

RC++ R+SR RR

10

RCHYTTPRISRRTRR

24

allorf_1461105_1461197

Leader:

MCRPKLPTGHSVIAG

Core:

RVAGECAYPSEIRGR

Core with dehydrations:

RVAGECysAYPDhaEIRGR

antiSMASH-DB 4.0 matches for allorf_1461105_1461197

100.0%

Lanthipeptides: allorf_1461105_1461197_lanthipeptide

1

RVAGECAYPSEIRGR

15

RVAGECAYPSEIRGR

1

RVAGECAYPSEIRGR

15

allorf_1461411_1461479

Leader:

MRTVGGA

Core:

GPRCTRTAHPSTQTG

Core with dehydrations:

GPRCysDhbRDhbAHPDhaDhbQDhbG

antiSMASH-DB 4.0 matches for allorf_1461411_1461479

100.0%

Lanthipeptides: allorf_1461411_1461479_lanthipeptide

1

GPRCTRTAHPSTQTG

15

GPRCTRTAHPSTQTG

1

GPRCTRTAHPSTQTG

15

23.1%

NZ_RYYV01000004.1:

Lanthipeptides: allorf_104699_104878_lanthipeptide

3

RCTRTAH

9

RC RTAH

2

RCLRTAH

8

allorf_1469834_1469980

Leader:

MGTVPIPLSMVSARPGGTGCCGGLRGRVAGS

Core:

GRGCCCGAAPSRKAARF

Core with dehydrations:

GRGCysCysCysGAAPDhaRKAARF

antiSMASH-DB 4.0 matches for allorf_1469834_1469980

100.0%

Lanthipeptides: allorf_1469834_1469980_lanthipeptide

1

GRGCCCGAAPSRKAARF

17

GRGCCCGAAPSRKAARF

1

GRGCCCGAAPSRKAARF

17

41.2%

NZ_BMQJ01000023.1:

Lanthipeptides: allorf_56466_56594_lanthipeptide

2

RGCCCGAAP

10

RG CC AAP

3

RGECCTAAP

11

20.6%

NZ_CP110813.1:

Lanthipeptides: allorf_3260520_3260750_lanthipeptide

2

RGCCCGAAPSRKAAR

16

RGC CG A + R

3

RGCLCGTAAGDRTHR

17

allorf_1473969_1474079

Leader:

MITGSLCAPEGDAESGA

Core:

ESVHPRCRPTGRVDFFRGP

Core with dehydrations:

EDhaVHPRCysRPDhbGRVDFFRGP

antiSMASH-DB 4.0 matches for allorf_1473969_1474079

100.0%

Lanthipeptides: allorf_1473969_1474079_lanthipeptide

1

ESVHPRCRPTGRVDFFRGP

19

ESVHPRCRPTGRVDFFRGP

1

ESVHPRCRPTGRVDFFRGP

19

18.8%

NZ_OCTY01000002.1:

Lanthipeptides: allorf_0873354_0873482_lanthipeptide

6

RCRPTGR

12

+CRPTGR

19

QCRPTGR

25

15.9%

NZ_ADVG01000001.1:

Lanthipeptides: allorf_1943256_1943423_lanthipeptide

2

SVHPRCRPTGRV

13

S HPRC+P V

27

SGHPRCKPGAAV

38

with 1 more sequence matches

15.2%

NZ_CP031749.1:

Lanthipeptides: allorf_5891878_5892102_lanthipeptide

1

ESVHPRCRPTGRVDFFR

17

E++HP T +FFR

10

ENLHPLRFATSLQNFFR

26

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.00 out of 20.47

Confidence: strong

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 24.69 out of 29.51

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

Additional weak hits (17):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.93 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.93 out of 29.38

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.74 out of 30.22

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.37 out of 30.22

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.27 out of 29.65

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.80 out of 23.43

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.91 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.78 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.11 out of 28.14

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.06 out of 33.73

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.91 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.79 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.65 out of 24.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.73 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.08 out of 29.65

Confidence: weak

(Thi)azol(in)e containing peptide predictions

(Thi)azol(in)e containing peptide(s)

EKD16_RS06070

Leader:

MDATNELDLNALEISDLIEDLNHSEETLSQVMGA

Core:

SCVGCACTCSSTSSSS

Core with dehydrations:

DhaCysVGCysACysDhbCysDhaDhaDhbDhaDhaDhaDha

antiSMASH-DB 4.0 matches for EKD16_RS06070

100.0%

Thiopeptides: EKD16_RS06070_thiopeptide

1

SCVGCACTCSSTSSSS

16

SCVGCACTCSSTSSSS

1

SCVGCACTCSSTSSSS

16

EKD16_RS06075

Leader:

MDATNELDLNALEISDLIEDSSYNDETLSQVMAA

Core:

SCTTTGCTTSSSSSSSS

Core with dehydrations:

DhaCysDhbDhbDhbGCysDhbDhbDhaDhaDhaDhaDhaDhaDhaDha

antiSMASH-DB 4.0 matches for EKD16_RS06075

100.0%

NZ_BMSO01000004.1and 2 others:

NZ_CP011497.1 Thiopeptides: ABB07_RS15875_thiopeptideNZ_CP036455.1 Thiopeptides: EKD16_RS06075_thiopeptide

Thiopeptides: IE184_RS11975_thiopeptide

1

SCTTTGCTTSSSSSSSS

17

SCTTTGCTTSSSSSSSS

1

SCTTTGCTTSSSSSSSS

17

41.2%

NC_018524.1:

Thiopeptides: B005_RS25790_thiopeptide

1

SCTTTGC

7

SCTTTGC

1

SCTTTGC

7

MIBiG 4.0 matches for EKD16_RS06075

13.5%

BGC0001229.4 (SBI_06989)and 1 other:

BGC0002110.3 (QKV49894.1) Lanthipeptide: birimositide

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

2

CTTTGCT

8

C TT CT

28

CPTTKCT

34

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Types:

Type I: Featuring genes encoding indolic acid side ring (series e)
Type II: Featuring the gene coding for quinaldic acid moiety formation (series a, b, and c)
Type III: No genes for synthesizing L-Trp derivatives (series d)

Lanthipeptides

RREFinder

TFBS Finder

Azole RiPPs

Core peptide predictions

EKD16_RS25235 - Class II

Cleavage pHMM score: -0.00

RODEO score: 0

Monoisotopic mass: 1982.9 Da

Molecular weight: 1984.3 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2002.3 Da N = 1

allorf_1457494_1457619 - Class II

Cleavage pHMM score: -7.10

RODEO score: 0

Monoisotopic mass: 2603.3 Da

Molecular weight: 2605.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2623.0 Da N = 1

allorf_1457522_1457644 - Class II

Cleavage pHMM score: -3.90

RODEO score: 0

Monoisotopic mass: 1502.9 Da

Molecular weight: 1503.8 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1521.8 Da N = 1; 1539.8 Da N = 2

allorf_1460897_1461118 - Class II

Cleavage pHMM score: -4.40

RODEO score: 0

Monoisotopic mass: 5117.6 Da

Molecular weight: 5120.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5138.6 Da N = 1; 5156.6 Da N = 2; 5174.6 Da N = 3; 5192.6 Da N = 4; 5210.7 Da N = 5; 5228.7 Da N = 6; 5246.7 Da N = 7; 5264.7 Da N = 8; 5282.7 Da N = 9

allorf_1461105_1461197 - Class II

Cleavage pHMM score: -4.70

RODEO score: 0

Monoisotopic mass: 1644.8 Da

Molecular weight: 1645.8 Da

Number of bridges: 1

allorf_1461411_1461479 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 1478.7 Da

Molecular weight: 1479.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1497.6 Da N = 1; 1515.6 Da N = 2; 1533.7 Da N = 3; 1551.7 Da N = 4

allorf_1469834_1469980 - Class II

Cleavage pHMM score: -5.60

RODEO score: 0

Monoisotopic mass: 1691.8 Da

Molecular weight: 1693.0 Da

Number of bridges: 1

allorf_1473969_1474079 - Class II

Cleavage pHMM score: -1.80

RODEO score: 0

Monoisotopic mass: 2176.1 Da

Molecular weight: 2177.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2195.5 Da N = 1

RRE predictions

: RRE type: Thiopeptide_F_RRE; Location: 12 - 90; Score: 41.8; RRE type: Thiopeptide_F_RRE; Location: 21 - 103; Score: 26.9

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
DosR	Hypoxia stress response regulator
VqsM	Regulator of quorum-sensing signalling systems

Core peptide predictions

EKD16_RS06070

Putative Type III

Cleavage pHMM score: 24.80

RODEO score: 29

Monoisotopic mass: 1468.5 Da

Molecular weight: 1469.6 Da

Predicted peptide features:
Central ring: pyridine trisubstituted

Tail reaction: dealkylation of C-Terminal residue; amidation

Alternative weights: (assuming N unmodified Ser/Thr residues); 1487.6 Da N = 1; 1505.6 Da N = 2; 1523.7 Da N = 3; 1541.7 Da N = 4; 1559.7 Da N = 5; 1577.7 Da N = 6; 1595.7 Da N = 7; 1613.8 Da N = 8; 1631.8 Da N = 9

EKD16_RS06075

Putative Type III

Cleavage pHMM score: 18.10

RODEO score: 31

Monoisotopic mass: 1569.6 Da

Molecular weight: 1570.6 Da

Predicted peptide features:
Central ring: pyridine trisubstituted

Macrocycle: 35-member

Tail reaction: dealkylation of C-Terminal residue; amidation

Alternative weights: (assuming N unmodified Ser/Thr residues); 1588.6 Da N = 1; 1606.6 Da N = 2; 1624.6 Da N = 3; 1642.6 Da N = 4; 1660.7 Da N = 5; 1678.7 Da N = 6; 1696.7 Da N = 7; 1714.7 Da N = 8; 1732.7 Da N = 9; 1750.8 Da N = 10; 1768.8 Da N = 11; 1786.8 Da N = 12; 1804.8 Da N = 13; 1822.8 Da N = 14

NZ_CP036455.1 - Region 5 - terpene

Download region SVG

Location: 1,905,144 - 1,934,887 nt. (total: 29,744 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKD16_RS08255: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: methyltransferase

EKD16_RS08275: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: synthetase

EKD16_RS08250: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.72	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.64	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.63	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.61	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.61	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000648.3	0.53	terpene	carotenoid	Myxococcus xanthus
BGC0000640.3	0.50	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000637.3	0.49	terpene	carotenoid	Corynebacterium glutamicum
BGC0000641.3	0.49	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.49	terpene	carotenoid	Enterobacteriaceae bacterium DC260

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.72	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.64	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.63	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.61	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.61	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000648.3	0.53	terpene	carotenoid	Myxococcus xanthus
BGC0000640.3	0.50	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000637.3	0.49	terpene	carotenoid	Corynebacterium glutamicum
BGC0000641.3	0.49	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.49	terpene	carotenoid	Enterobacteriaceae bacterium DC260

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

EKD16_RS08250: PT_FPPS_like

Domain 1 (86 - 400): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

EKD16_RS08260: PT_phytoene_like

Domain 1 (17 - 320): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → PSPP; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

EKD16_RS08280: Lycopene_cycl

Domain 1 (5 - 381): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.35 out of 20.47

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.02 out of 25.31

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.99 out of 20.97

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (21):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.50 out of 25.75

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.23 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.37 out of 29.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.95 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.07 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.42 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.86 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.19 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.40 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.01 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.20 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.50 out of 28.14

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.70 out of 30.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.05 out of 30.97

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.51 out of 20.97

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.87 out of 36.53

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.02 out of 26.85

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.22 out of 26.12

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.95 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.80 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.89 out of 29.65

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CatR	H2O2-responsive repressor
CcpA	Catabolite control protein A
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP036455.1 - Region 6 - terpene-precursor

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Location: 2,283,698 - 2,304,768 nt. (total: 21,071 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

EKD16_RS09895: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

Transferases: carbamoyltransferase phosphorylase

EKD16_RS09905: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

EKD16_RS09950: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

Ligases: ligase synthase synthetase

EKD16_RS09910: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EKD16_RS09925: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

EKD16_RS09960: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002286.3	0.56	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002405.2	0.46	saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000649.3	0.42	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000664.5	0.41	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000597.4	0.40	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.40	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0001529.5	0.40	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0002343.3	0.39	other (other), ribosomal	ammosester A, ammosester B, ammosester C, ammosamaic acid cogener	Streptomyces uncialis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002286.3	0.56	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002405.2	0.46	saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000649.3	0.42	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000664.5	0.41	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000597.4	0.40	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.40	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0001529.5	0.40	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0002343.3	0.39	other (other), ribosomal	ammosester A, ammosester B, ammosester C, ammosamaic acid cogener	Streptomyces uncialis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

EKD16_RS09925: PT_FPPS_like

Domain 1 (49 - 348): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.54 out of 24.78

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.83 out of 20.47

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (13):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.17 out of 26.06

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.14 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.45 out of 20.01

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.84 out of 30.98

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.59 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.65 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.96 out of 24.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.34 out of 28.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.71 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.83 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP036455.1 - Region 7 - NRP-metallophore,NRPS

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Location: 3,239,084 - 3,309,121 nt. (total: 70,038 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

EKD16_RS14010: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

EKD16_RS14030: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: PPTase acetyltransferase halogenase

EKD16_RS13915: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

EKD16_RS14000: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

EKD16_RS14115: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: hydrolase

EKD16_RS14015: isochorismatase

smcogs: SMCOG1027: isochorismatase

Lyases: hydratase

EKD16_RS14075: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Isomerases: isomerase

EKD16_RS14025: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

EKD16_RS13905: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

EKD16_RS13985: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EKD16_RS13995: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EKD16_RS14020: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002017.3	0.97	NRPS	potensibactin	Nocardiopsis potens DSM 45234
BGC0002341.2	0.95	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001833.4	0.91	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002608.2	0.90	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002135.2	0.86	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.85	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001133.4	0.84	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.83	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001185.5	0.83	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0001128.4	0.83	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002017.3	0.78	NRPS	potensibactin	Nocardiopsis potens DSM 45234
BGC0002341.2	0.77	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001133.4	0.73	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001185.5	0.73	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.29 out of 36.53

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.54 out of 20.01

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.85 out of 20.97

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 28.73 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 28.71 out of 36.53

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.54 out of 24.78

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 22.19 out of 27.04

Confidence: medium

Additional weak hits (22):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.20 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.95 out of 31.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.58 out of 36.54

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.75 out of 29.65

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.88 out of 25.75

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.53 out of 21.84

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.57 out of 39.62

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.68 out of 39.62

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.85 out of 35.63

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.21 out of 32.93

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.86 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.12 out of 36.53

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.09 out of 26.85

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.76 out of 21.84

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.41 out of 28.19

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 21.07 out of 32.38

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.71 out of 31.73

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.49 out of 28.19

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.93 out of 27.04

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.00 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.07 out of 28.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 9, location 3239083 - 3309121:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Gly - Ser - D-Ser) + (X - OH-Orn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKD16_RS13985: X - OH-Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (591..993): X

nrpys: Ac-OH-Orn, OH-Orn

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTAGFDVAFWEMGLLAGTEFNCYGPTEATVNATL
Nearest Stachelhaus code(s):: Ac-OH-Orn DVWALGAVNK (74% 8Å match); OH-Orn DVWALGAVNK (71% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1661..2070): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSHWFDAAWQPWEVGVGGEVNLYGPTETTVDALA
Nearest Stachelhaus code(s):: OH-Orn DAQEGGLVDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

EKD16_RS13995: Gly - Gly - Ser - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (493..893): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FDMTFDISALELQALVGGEVNLYGPTETTIWSTA
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1552..1948): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FDMTFDISALELQALVGGEVNLYGPTETTIWSTA
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2624..3030): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFASGEANLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3696..4101): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFASGEANLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

EKD16_RS14020: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (31..447): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SASLFPMSSPGGAVQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PMPAQGVVNK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
CcpA	Catabolite control protein A
DasR	N-acetylglucosamine dependent repressor
DmdR1	Iron(II)-dependent repressor
LexA variant 2	Repressor of DNA damage response

NZ_CP036455.1 - Region 8 - terpene-precursor

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Location: 3,429,254 - 3,450,294 nt. (total: 21,041 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: transketolase

EKD16_RS14645: transketolase

smcogs: SMCOG1204: transketolase

Ligases: ligase synthase synthetase

EKD16_RS14620: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

EKD16_RS14630: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

EKD16_RS14650: carboxylate-amine ligase

smcogs: SMCOG1165: carboxylate-amine ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002162.3	0.50	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0000657.5	0.46	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000665.5	0.43	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000666.5	0.41	terpene	xiamycin A	Streptomyces sp. SCSIO 02999
BGC0000663.5	0.39	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0001075.5	0.38	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0001156.5	0.38	terpene	platencin	Streptomyces platensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002162.3	0.50	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0000657.5	0.46	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000665.5	0.43	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000666.5	0.41	terpene	xiamycin A	Streptomyces sp. SCSIO 02999
BGC0000663.5	0.39	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0001075.5	0.38	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0001156.5	0.38	terpene	platencin	Streptomyces platensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

EKD16_RS14630: PT_FPPS_like

Domain 1 (59 - 344): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.86 out of 18.57

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.34 out of 20.97

Confidence: medium

Additional weak hits (17):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.97 out of 26.06

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.52 out of 27.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.32 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.03 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.79 out of 19.52

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.37 out of 30.22

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.70 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.93 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.76 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.86 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.77 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.00 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.22 out of 29.38

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.02 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.26 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
ToxT	Regulator of MSHA biosynthesis
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP036455.1 - Region 9 - T1PKS

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Location: 3,470,183 - 3,532,533 nt. (total: 62,351 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKD16_RS14860: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

EKD16_RS14870: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

EKD16_RS14940: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: aminotransferase kinase

EKD16_RS14790: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

EKD16_RS14945: glycerol kinase

smcogs: SMCOG1210: glycerol kinase

Ligases: synthase

EKD16_RS14865: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Translocases: transport

EKD16_RS14855: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.63	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.63	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.45 out of 29.72

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.42 out of 20.47

Confidence: medium

Additional weak hits (28):

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.54 out of 19.26

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.41 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.63 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.61 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.32 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.66 out of 26.06

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.80 out of 19.26

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.19 out of 20.97

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.29 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.27 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.93 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.24 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.60 out of 26.06

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.06 out of 36.54

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.00 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.47 out of 27.78

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.54 out of 19.26

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.33 out of 20.97

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.60 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.10 out of 30.11

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.32 out of 26.06

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.81 out of 29.65

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.86 out of 19.52

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.73 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.23 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 16.88 out of 30.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.52 out of 29.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.41 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 11, location 3470182 - 3532533:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-redmal) + (Me-ohmal) + (pk)
Non-colinear ordering:: EKD16_RS14870 + EKD16_RS14865 + EKD16_RS14860

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKD16_RS14860: pk

PKS_AT (596..897): pk

ATSignature: Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 180.8
Methylmalonyl-CoA: 178.7
Methoxymalonyl-CoA: 139.9
2-Methylbutyryl-CoA: 116.7
trans-1,2-CPDA: 116.4
Propionyl-CoA: 92.7
CHC-CoA: 86.1
Malonyl-CoA: 81.3
Isobutyryl-CoA: 64.6
Benzoyl-CoA: 53.1
Acetyl-CoA: 33.7
fatty_acid: 30.1
3-Methylbutyryl-CoA: 19.6
inactive: 19.5

EKD16_RS14865: mmal

PKS_AT (592..899): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.5
Ethylmalonyl-CoA: 186.4
Methoxymalonyl-CoA: 163.4
2-Methylbutyryl-CoA: 146.3
trans-1,2-CPDA: 105.5
Propionyl-CoA: 97.4
Malonyl-CoA: 95.1
Isobutyryl-CoA: 76.2
CHC-CoA: 70.8
Benzoyl-CoA: 47.9
Acetyl-CoA: 44.6
inactive: 37.3
3-Methylbutyryl-CoA: 27.8
fatty_acid: 10.5

EKD16_RS14870: mal - mmal

PKS_AT (546..838): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 50.2
Methoxymalonyl-CoA: 22.8
Isobutyryl-CoA: 15.5
Acetyl-CoA: 9.3
Methylmalonyl-CoA: 7.6
Benzoyl-CoA: 4.0
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
CHC-CoA: -1.4
Propionyl-CoA: -1.5

PKS_AT (1602..1899): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.6
Ethylmalonyl-CoA: 163.8
Methoxymalonyl-CoA: 146.8
2-Methylbutyryl-CoA: 120.5
Malonyl-CoA: 97.9
trans-1,2-CPDA: 96.8
Propionyl-CoA: 85.5
CHC-CoA: 82.5
Isobutyryl-CoA: 79.6
Benzoyl-CoA: 51.1
Acetyl-CoA: 33.4
inactive: 27.6
3-Methylbutyryl-CoA: 22.1
fatty_acid: 10.9

TFBS definitions

Regulator	Description
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP036455.1 - Region 10 - T1PKS,T3PKS

Download region SVG

Location: 3,544,203 - 3,647,129 nt. (total: 102,927 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase

EKD16_RS15100: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EKD16_RS15125: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

EKD16_RS15210: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

EKD16_RS15280: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EKD16_RS15305: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Transferases: acylase aminotransferase glycosyltransferase sulfotransferase transferase

EKD16_RS15110: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

EKD16_RS15115: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

EKD16_RS15130: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

EKD16_RS15175: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

EKD16_RS15185: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

EKD16_RS15190: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

EKD16_RS15195: Sulfotransferase domain

extras: Sulfotransfer_4: Sulfotransferase domain

Hydrolases: esterase hydrolase

EKD16_RS15060: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

EKD16_RS15095: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

EKD16_RS15170: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

EKD16_RS15105: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

EKD16_RS15225: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

EKD16_RS15285: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

EKD16_RS26150: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.47	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.46	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.45	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000898.5	0.42	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000790.3	0.39	saccharide	O-antigen	Escherichia coli
BGC0000041.3	0.38	PKS	oronofacic acid	Pseudomonas syringae
BGC0001817.5	0.38	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000794.5	0.37	saccharide	S-layer glycan	Geobacillus stearothermophilus
BGC0000827.3	0.35	other (other)	staurosporine	Salinispora arenicola CNS-205
BGC0000773.3	0.35	saccharide	lipopolysaccharide	Escherichia coli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000018.5	0.65	PKS	angolamycin	Streptomyces eurythermus
BGC0000113.5	0.64	PKS	niddamycin	Streptomyces caelestis
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000067.5	0.63	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.23 out of 20.97

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.21 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.48 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.00 out of 23.79

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.91 out of 28.19

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.13 out of 28.19

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 24.69 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 26.43 out of 30.11

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (38):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.07 out of 27.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.37 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.79 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.96 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.76 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.59 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.92 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.59 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.72 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.37 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.87 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.80 out of 29.38

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.03 out of 36.54

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.45 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.43 out of 28.14

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.51 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.99 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.93 out of 27.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.59 out of 18.57

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.37 out of 30.97

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.88 out of 25.75

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 21.71 out of 33.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.93 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.63 out of 29.38

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.00 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.13 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.56 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.60 out of 31.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.66 out of 29.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.15 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.48 out of 29.38

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.15 out of 20.97

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.80 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.65 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.11 out of 28.19

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.32 out of 24.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.83 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 12, location 3544202 - 3647129:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ccemal - ohmal) + (pk) + (Me-ohmal) + (Me-ohmal - ccmal) + (ccmal)
Non-colinear ordering:: EKD16_RS26150 + EKD16_RS15115 + EKD16_RS15110 + EKD16_RS15105 + EKD16_RS15100

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 13, location 3544202 - 3622894:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ccemal - ohmal) + (pk) + (Me-ohmal) + (Me-ohmal - ccmal) + (ccmal)
Non-colinear ordering:: EKD16_RS26150 + EKD16_RS15115 + EKD16_RS15110 + EKD16_RS15105 + EKD16_RS15100

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKD16_RS15100: mal

PKS_AT (568..866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.9
inactive: 94.5
Methoxymalonyl-CoA: 57.7
Methylmalonyl-CoA: 57.6
Propionyl-CoA: 28.6
Ethylmalonyl-CoA: 25.8
Isobutyryl-CoA: 23.2
fatty_acid: 21.9
Benzoyl-CoA: 15.8
trans-1,2-CPDA: 15.6
Acetyl-CoA: 13.7
CHC-CoA: 10.5
2-Methylbutyryl-CoA: 8.2
3-Methylbutyryl-CoA: 0.0

EKD16_RS15105: mmal - mal

PKS_AT (562..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.5
Ethylmalonyl-CoA: 195.7
Methoxymalonyl-CoA: 167.2
2-Methylbutyryl-CoA: 119.9
trans-1,2-CPDA: 116.6
CHC-CoA: 93.2
Propionyl-CoA: 90.9
Malonyl-CoA: 90.9
Isobutyryl-CoA: 63.2
Benzoyl-CoA: 50.2
Acetyl-CoA: 39.4
fatty_acid: 37.4
3-Methylbutyryl-CoA: 34.0
inactive: 17.1

PKS_AT (2036..2334): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 193.4
inactive: 86.3
Methoxymalonyl-CoA: 54.9
Methylmalonyl-CoA: 53.4
Propionyl-CoA: 28.0
Isobutyryl-CoA: 21.6
Ethylmalonyl-CoA: 21.2
Acetyl-CoA: 16.4
fatty_acid: 15.2
2-Methylbutyryl-CoA: 12.9
trans-1,2-CPDA: 11.9
Benzoyl-CoA: 9.3
CHC-CoA: 8.6
3-Methylbutyryl-CoA: 0.0

EKD16_RS15110: mmal

PKS_AT (603..902): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 185.3
Ethylmalonyl-CoA: 182.3
Methoxymalonyl-CoA: 146.4
2-Methylbutyryl-CoA: 133.2
trans-1,2-CPDA: 113.5
Propionyl-CoA: 94.2
CHC-CoA: 90.7
Malonyl-CoA: 85.2
Benzoyl-CoA: 63.2
Isobutyryl-CoA: 51.7
Acetyl-CoA: 46.0
fatty_acid: 26.2
inactive: 21.3
3-Methylbutyryl-CoA: 13.7

EKD16_RS15115: pk

PKS_AT (567..860): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 220.2
Methylmalonyl-CoA: 214.4
Methoxymalonyl-CoA: 168.4
2-Methylbutyryl-CoA: 141.2
trans-1,2-CPDA: 128.1
Propionyl-CoA: 116.2
Malonyl-CoA: 115.1
CHC-CoA: 112.5
Isobutyryl-CoA: 77.9
Benzoyl-CoA: 77.7
Acetyl-CoA: 70.5
inactive: 36.3
fatty_acid: 28.4
3-Methylbutyryl-CoA: 20.1

EKD16_RS26150: mal - emal - mal

PKS_AT (528..826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.3
inactive: 106.5
Methoxymalonyl-CoA: 54.3
Methylmalonyl-CoA: 49.3
Isobutyryl-CoA: 35.1
Propionyl-CoA: 24.8
Ethylmalonyl-CoA: 19.0
Acetyl-CoA: 16.6
Benzoyl-CoA: 12.2
2-Methylbutyryl-CoA: 5.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1557..1854): emal

ATSignature: Ethylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 188.2
Methylmalonyl-CoA: 177.2
Methoxymalonyl-CoA: 162.4
trans-1,2-CPDA: 132.5
2-Methylbutyryl-CoA: 131.0
Propionyl-CoA: 106.9
CHC-CoA: 95.2
Malonyl-CoA: 90.3
Isobutyryl-CoA: 75.0
Benzoyl-CoA: 65.4
Acetyl-CoA: 54.0
3-Methylbutyryl-CoA: 30.3
fatty_acid: 25.8
inactive: 22.0

PKS_AT (3266..3560): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 196.2
inactive: 89.1
Methoxymalonyl-CoA: 61.6
Methylmalonyl-CoA: 43.0
Propionyl-CoA: 29.1
Acetyl-CoA: 15.8
Isobutyryl-CoA: 11.9
fatty_acid: 10.6
2-Methylbutyryl-CoA: 8.6
CHC-CoA: 6.3
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.3

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CcpA	Catabolite control protein A
CelR	Cellobiose uptake repressor
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

NZ_CP036455.1 - Region 11 - ectoine

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Location: 3,655,290 - 3,665,664 nt. (total: 10,375 nt) Show pHMM detection rules used

ectoine: ectoine_synt

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKD16_RS15390: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Ligases: ligase

EKD16_RS15365: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001778.5	0.54	other (other)	showdomycin	Streptomyces showdoensis
BGC0000858.3	0.48	other (other)	ectoine	Methylobacter marinus
BGC0001073.5	0.45	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0000131.5	0.44	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0002020.3	0.44	NRPS, saccharide	photolose, oligosaccharide 1 (GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc), oligosaccharide 2 (GlcNAc-β(1-4)-GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc), oligosaccharide 3 (GlcNAc-β(1-4)-GlcNAc-β(1-4)-GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc), oligosaccharide 4 (GlcN-β(1-4)-GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc), oligosaccharide 5 (6-phosphoGlcN-β(1-4)-GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc)	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002097.3	0.43	NRPS, PKS, saccharide	kosinostatin	Micromonospora sp. 28ISP2-46
BGC0001895.4	0.41	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0002000.4	0.41	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0001463.4	0.41	other (other)	2,3,4,5-tetrabromopyrrole	Pseudoalteromonas piscicida
BGC0001464.3	0.41	other (other)	tetrabromopyrrole	Pseudoalteromonas sp. PS5

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001778.5	0.54	other (other)	showdomycin	Streptomyces showdoensis
BGC0000858.3	0.48	other (other)	ectoine	Methylobacter marinus
BGC0001073.5	0.45	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0000131.5	0.44	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0002020.3	0.44	NRPS, saccharide	photolose, oligosaccharide 1 (GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc), oligosaccharide 2 (GlcNAc-β(1-4)-GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc), oligosaccharide 3 (GlcNAc-β(1-4)-GlcNAc-β(1-4)-GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc), oligosaccharide 4 (GlcN-β(1-4)-GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc), oligosaccharide 5 (6-phosphoGlcN-β(1-4)-GlcNAc-β(1-4)-1,6-anhydro-β-GlcNAc)	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002097.3	0.43	NRPS, PKS, saccharide	kosinostatin	Micromonospora sp. 28ISP2-46
BGC0001895.4	0.41	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0002000.4	0.41	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0001463.4	0.41	other (other)	2,3,4,5-tetrabromopyrrole	Pseudoalteromonas piscicida
BGC0001464.3	0.41	other (other)	tetrabromopyrrole	Pseudoalteromonas sp. PS5

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.37 out of 20.47

Confidence: strong

Additional weak hits (4):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.33 out of 20.01

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.67 out of 20.01

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.73 out of 29.25

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.22 out of 32.93

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP036455.1 - Region 12 - NRPS-like,RiPP-like,lanthipeptide-class-iii

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Location: 4,265,866 - 4,314,656 nt. (total: 48,791 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: back translocase hydrolase

EKD16_RS17955: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

EKD16_RS18030: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: isomerase

EKD16_RS18035: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

EKD16_RS18040: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	1.10	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000513.3	0.58	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.53	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.53	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000496.5	0.48	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000518.3	0.43	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002700.2	0.39	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002115.3	0.39	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0000546.3	0.36	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0000553.3	0.35	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.81	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000513.3	0.65	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.60	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000496.5	0.58	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000518.3	0.57	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000936.5	0.52	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002700.2	0.52	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000546.3	0.50	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0001444.5	0.49	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EKD16_RS17980

EKD16_RS17975

Leader:

MSLLDLQSLETTTEAHGEVS

Core:

AGSEASLLLCGDSSLSLTTCN

Core with dehydrations:

AGDhaEADhaLLLCysGDDhaDhaLDhaLDhbDhbCysN

antiSMASH-DB 4.0 matches for EKD16_RS17975

100.0%

BGC0000553.3 (SSGG_RS09925):

Lanthipeptides: EKD16_RS17975_lanthipeptide

1

AGSEASLLLCGDSSLSLTTCN

21

AGSEASLLLCGDSSLSLTTCN

1

AGSEASLLLCGDSSLSLTTCN

21

85.7%

NC_018750.1and 75 others:

NC_018750.1 Lanthipeptides: SVEN_RS32110_lanthipeptideNZ_BBNO01000003.1 Lanthipeptides: TU31_RS37940_lanthipeptideNZ_BMSV01000002.1 Lanthipeptides: IE106_RS06150_lanthipeptideNZ_BMTO01000007.1 Lanthipeptides: IE136_RS15615_lanthipeptideNZ_BMTP01000009.1 Lanthipeptides: IE109_RS18470_lanthipeptideNZ_BMUN01000001.1 Lanthipeptides: IE238_RS00740_lanthipeptideNZ_CP007699.2 Lanthipeptides: T261_RS39185_lanthipeptideNZ_CP009754.1 Lanthipeptides: NI25_RS05015_lanthipeptideNZ_CP015866.1 Lanthipeptides: Spa2297_RS27800_lanthipeptideNZ_CP017157.1 Lanthipeptides: SL103_RS15895_lanthipeptideNZ_CP019457.1 Lanthipeptides: BXT88_RS03410_lanthipeptideNZ_CP019724.1 Lanthipeptides: B1H29_RS35355_lanthipeptideNZ_CP019779.1 Lanthipeptides: STRMOE7_RS03775_lanthipeptideNZ_CP023691.1 Lanthipeptides: CP981_RS03050_lanthipeptideNZ_CP023700.1 Lanthipeptides: CP969_RS04700_lanthipeptideNZ_CP027299.1 Lanthipeptides: C1N79_RS34970_lanthipeptideNZ_CP029042.1 Lanthipeptides: DDE74_RS03525_lanthipeptideNZ_CP029195.1 Lanthipeptides: DEJ45_RS04995_lanthipeptideNZ_CP029195.1 Lanthipeptides: DEJ45_RS04995_lanthipeptideNZ_CP029196.1 Lanthipeptides: DEJ44_RS30395_lanthipeptideNZ_CP029196.1 Lanthipeptides: DEJ44_RS30395_lanthipeptideNZ_CP029541.1 Lanthipeptides: DKG71_RS05245_lanthipeptideNZ_CP034279.1 Lanthipeptides: EIZ62_RS28660_lanthipeptideNZ_CP034353.1 Lanthipeptides: D1J63_RS04900_lanthipeptideNZ_CP039123.1 Lanthipeptides: E5N77_RS35250_lanthipeptideNZ_CP043317.1 Lanthipeptides: FYC74_RS09720_lanthipeptideNZ_CP046905.1 Lanthipeptides: GPZ77_RS32930_lanthipeptideNZ_CP047140.1 Lanthipeptides: GLX30_RS11420_lanthipeptideNZ_CP050976.1 Lanthipeptides: HEP86_RS04470_lanthipeptideNZ_CP054920.1 Lanthipeptides: HUT15_RS04615_lanthipeptideNZ_CP060825.1 Lanthipeptides: IAG43_RS32355_lanthipeptideNZ_CP074111.1 Lanthipeptides: KE639_RS31630_lanthipeptideNZ_CP084681.1 Lanthipeptides: KI385_RS00885_lanthipeptideNZ_CP085300.1 Lanthipeptides: LJA35_RS05220_lanthipeptideNZ_CP086217.1 Lanthipeptides: LJ741_RS03435_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS09690_lanthipeptideNZ_CP098437.1 Lanthipeptides: NCG97_RS34740_lanthipeptideNZ_CP099468.1 Lanthipeptides: NFX46_RS16770_lanthipeptideNZ_CP101137.1 Lanthipeptides: M6G08_RS24535_lanthipeptideNZ_CP102332.1 Lanthipeptides: NRO40_RS01140_lanthipeptideNZ_CP102609.1 Lanthipeptides: NUT86_RS03980_lanthipeptideNZ_CP104697.1 Lanthipeptides: N6Q81_RS33560_lanthipeptideNZ_CP114202.1 Lanthipeptides: STRLI_RS35870_lanthipeptideNZ_CP114203.1 Lanthipeptides: STRNI_RS37360_lanthipeptideNZ_CP114205.1 Lanthipeptides: STRTU_RS33380_lanthipeptideNZ_JACHJE010000006.1 Lanthipeptides: FHS32_RS15850_lanthipeptideNZ_JACSCH020000032.1 Lanthipeptides: E0F75_RS38565_lanthipeptideNZ_JACSCH020000032.1 Lanthipeptides: E0F75_RS38565_lanthipeptideNZ_JACSCH020000032.1 Lanthipeptides: E0F75_RS38565_lanthipeptideNZ_JACWAA010000001.1 Lanthipeptides: ICA23_RS00800_lanthipeptideNZ_JADMLH010000003.1 Lanthipeptides: IT780_RS09875_lanthipeptideNZ_JAFEJA010000002.1 Lanthipeptides: JE024_RS34830_lanthipeptideNZ_JAGPYC010000033.1 Lanthipeptides: KBZ00_RS31440_lanthipeptideNZ_JAHLXF010000022.1 Lanthipeptides: KQY13_RS30555_lanthipeptideNZ_JAIHBQ010000001.1 Lanthipeptides: K4902_RS04515_lanthipeptideNZ_JAJJMU010000026.1 Lanthipeptides: LOC25_RS31275_lanthipeptideNZ_JAJNOJ010000002.1 Lanthipeptides: MBT42_RS06255_lanthipeptideNZ_JAJNOJ010000002.1 Lanthipeptides: MBT42_RS06255_lanthipeptideNZ_JAMGSL010000004.1 Lanthipeptides: M5362_RS09920_lanthipeptideNZ_JAMGSL010000004.1 Lanthipeptides: M5362_RS09920_lanthipeptideNZ_JAPKNL010000004.1 Lanthipeptides: OSI21_RS07750_lanthipeptideNZ_JH993790.1 Lanthipeptides: STRVIR_RS0131905_lanthipeptideNZ_KN039948.1 Lanthipeptides: FM21_RS29525_lanthipeptideNZ_LIPF01000004.1 Lanthipeptides: AMK11_RS16660_lanthipeptideNZ_LIVV01000002.1 Lanthipeptides: AMK27_RS08700_lanthipeptideNZ_LIVV01000002.1 Lanthipeptides: AMK27_RS08700_lanthipeptideNZ_LIVX01000004.1 Lanthipeptides: AMK29_RS15650_lanthipeptideNZ_LIWB01000010.1 Lanthipeptides: AMK33_RS24360_lanthipeptideNZ_LIYH01000004.1 Lanthipeptides: AMK26_RS17765_lanthipeptideNZ_MPOH02000012.1 Lanthipeptides: BM536_RS17150_lanthipeptideNZ_NBCN01000003.1 Lanthipeptides: BKD26_RS06570_lanthipeptideNZ_NNBP01000011.1 Lanthipeptides: CG747_RS19290_lanthipeptideNZ_NNBP01000011.1 Lanthipeptides: CG747_RS19290_lanthipeptideNZ_RAIF01000032.1 Lanthipeptides: EBH09_RS35480_lanthipeptideNZ_SOPG01000008.1 Lanthipeptides: E0I08_RS17090_lanthipeptide

Lanthipeptides: SVEN_RS32110_lanthipeptide

2

GSEASLLLCGDSSLSLTTCN

21

GS ASLLLCGDSSLS+TTCN

2

GSRASLLLCGDSSLSVTTCN

21

85.7%

NZ_BBOK01000011.1and 26 others:

NZ_BEWD01000001.1 Lanthipeptides: Sru02f_RS01660_lanthipeptideNZ_BMSL01000016.1 Lanthipeptides: IE215_RS23330_lanthipeptideNZ_BMUC01000023.1 Lanthipeptides: IE172_RS32210_lanthipeptideNZ_BMVQ01000005.1 Lanthipeptides: IE283_RS17035_lanthipeptideNZ_BMWI01000025.1 Lanthipeptides: IE253_RS33945_lanthipeptideNZ_CM002280.1 Lanthipeptides: M877_RS44575_lanthipeptideNZ_CP012382.1 Lanthipeptides: SAM23877_RS30085_lanthipeptideNZ_CP012949.1 Lanthipeptides: SAM40697_RS29010_lanthipeptideNZ_CP018047.1 Lanthipeptides: BBN63_RS03065_lanthipeptideNZ_CP042324.1 Lanthipeptides: FQ762_RS34495_lanthipeptideNZ_CP043959.1 Lanthipeptides: F3L20_RS19105_lanthipeptideNZ_CP045547.1 Lanthipeptides: GEV49_RS05730_lanthipeptideNZ_CP048397.1 Lanthipeptides: SSPS47_RS31455_lanthipeptideNZ_CP054939.1 Lanthipeptides: HUT14_RS29815_lanthipeptideNZ_CP094918.1 Lanthipeptides: MTR79_RS16715_lanthipeptideNZ_GG657756.1 Lanthipeptides: SSPG_RS05020_lanthipeptideNZ_JAAIFS010000001.1 Lanthipeptides: GUR47_RS02255_lanthipeptideNZ_KZ195574.1 Lanthipeptides: B9W62_RS05695_lanthipeptideNZ_LT629768.1 Lanthipeptides: BLU60_RS34110_lanthipeptideNZ_MQUH01000025.1 Lanthipeptides: BSZ07_RS33280_lanthipeptideNZ_RAII01000001.1 Lanthipeptides: EBH12_RS10360_lanthipeptideNZ_RSEG01000006.1 Lanthipeptides: EIA57_RS12710_lanthipeptideNZ_SOAV01000001.1 Lanthipeptides: EV562_RS04520_lanthipeptideNZ_VIUV01000020.1 Lanthipeptides: FB570_RS34060_lanthipeptideNZ_WHZR01000001.1 Lanthipeptides: STPH1_RS04005_lanthipeptideNZ_WXVT01000001.1 Lanthipeptides: STPH2_RS03995_lanthipeptide

Lanthipeptides: APK71_RS25550_lanthipeptide

2

GSEASLLLCGDSSLSLTTCN

21

GS ASLLLCGDSSLS+TTCN

2

GSRASLLLCGDSSLSITTCN

21

with 75 more sequence matches

Only the first 10 are displayed here.

85.7%

NZ_BMTU01000009.1and 1 other:

NZ_JACHJI010000005.1 Lanthipeptides: FHS37_RS15430_lanthipeptide

Lanthipeptides: IE118_RS22890_lanthipeptide

1

AGSEASLLLCGDSSLSLTTCN

21

+GS ASLLLCGDSSLS+TTCN

1

SGSRASLLLCGDSSLSVTTCN

21

85.7%

NZ_FOET01000001.1and 2 others:

NZ_FOSG01000005.1 Lanthipeptides: BM237_RS08435_lanthipeptideNZ_PDKQ02000044.1 Lanthipeptides: BFM75_RS16855_lanthipeptide

Lanthipeptides: BMY40_RS02540_lanthipeptide

1

AGSEASLLLCGDSSLSLTTCN

21

GSEASLLLCGDSSLS+TTC

1

GGSEASLLLCGDSSLSVTTCR

21

85.7%

NZ_GG657758.1and 1 other:

NZ_JACHEM010000005.1 Lanthipeptides: HNQ79_RS11445_lanthipeptide

Lanthipeptides: SSRG_RS03855_lanthipeptide

1

AGSEASLLLCGDSSLSLTTCN

21

+GS ASLLLCGDSSLS+TTCN

1

SGSRASLLLCGDSSLSITTCN

21

85.7%

NZ_QGGR01000008.1:

Lanthipeptides: BC793_RS19230_lanthipeptide

1

AGSEASLLLCGDSSLSLTTCN

21

GS+ASLLLCGDSSLS+TTCN

1

GGSQASLLLCGDSSLSVTTCN

21

81.0%

NZ_BNCA01000002.1and 1 other:

NZ_CP049782.1 Lanthipeptides: G7Z12_RS34445_lanthipeptide

Lanthipeptides: IE314_RS02760_lanthipeptide

2

GSEASLLLCGDSSLSLTTCN

21

GS ASLLLCGDSSLS++TCN

2

GSRASLLLCGDSSLSVSTCN

21

81.0%

NZ_CP048835.1:

Lanthipeptides: C8250_RS00390_lanthipeptide

1

AGSEASLLLCGDSSLSLTTCN

21

GS ASLLLCG+SSLS+TTCN

1

GGSRASLLLCGNSSLSVTTCN

21

81.0%

NZ_JAEIOJ010000010.1:

Lanthipeptides: IMZ35_RS23775_lanthipeptide

5

ASLLLCGDSSLSLTTCN

21

ASLLLCGDSSLSLTTCN

1

ASLLLCGDSSLSLTTCN

17

81.0%

NZ_KI911536.1:

Lanthipeptides: B042_RS0117070_lanthipeptide

1

AGSEASLLLCGDSSLSLTTC

20

A S ASLLLCGDSSLS+TTC

1

ATSTASLLLCGDSSLSVTTC

20

81.0%

NZ_VWVV01000017.1and 1 other:

NZ_VWVW01000007.1 Lanthipeptides: FYW96_RS09550_lanthipeptide

Lanthipeptides: FYW95_RS16780_lanthipeptide

3

SEASLLLCGDSSLSLTTCN

21

S ASLLLCGDSSLSLTTC

2

SAASLLLCGDSSLSLTTCR

20

78.3%

NZ_CP102509.1:

Lanthipeptides: JIW86_RS37035_lanthipeptide

1

AGSEASLLLCGDSSLSLTTCN

21

GS ASLLLCGDSSLSLTTC+

3

GGSRASLLLCGDSSLSLTTCH

23

MIBiG 4.0 matches for EKD16_RS17975

50.0%

Lanthipeptide: SRO15-2212

2

GSEASLLLCGDSSLSLTTCN

21

GS+ SLL+C SSLS+ C

2

GSQVSLLVCEYSSLSVVLCT

21

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.67 out of 20.97

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.45 out of 27.78

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.76 out of 30.11

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.54 out of 20.01

Confidence: medium

Additional weak hits (33):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.40 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.71 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.82 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.05 out of 28.14

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.76 out of 18.57

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.69 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.38 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.30 out of 26.06

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.19 out of 30.97

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.73 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.26 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.05 out of 28.14

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.15 out of 25.75

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.84 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.23 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.78 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.99 out of 18.57

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.85 out of 21.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.63 out of 31.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.01 out of 30.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 20.18 out of 30.97

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 22.65 out of 32.93

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.27 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.38 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.81 out of 28.14

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.34 out of 29.25

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.77 out of 39.62

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.35 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.18 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.32 out of 29.25

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.53 out of 33.14

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.87 out of 32.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.55 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

RREFinder

TFBS Finder

Core peptide predictions

EKD16_RS17975 - Class III

Cleavage pHMM score: 14.60

RODEO score: 0

Monoisotopic mass: 1914.9 Da

Molecular weight: 1916.1 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1934.1 Da N = 1; 1952.1 Da N = 2; 1970.2 Da N = 3; 1988.2 Da N = 4; 2006.2 Da N = 5

Predicted core structure(s)

For candidate cluster 16, location 4265865 - 4314656:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 18, location 4271257 - 4314656:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKD16_RS18040: X

AMP-binding (41..473): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RAVGVEYTVVDAMILVDGV--------GTVCATF
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

RRE predictions

: RRE type: Goadsporin_CD_RRE; Location: 14 - 112; Score: 62.7; RRE type: NHLP_CD_RRE; Location: 13 - 96; Score: 53.4; RRE type: Cyanobactin_D_RRE; Location: 14 - 89; Score: 42.5

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
ArgR	Regulator of arginine biosynthesis genes
CcpA	Catabolite control protein A
CelR	Cellobiose uptake repressor
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria

NZ_CP036455.1 - Region 13 - lanthipeptide-class-i

Download region SVG

Location: 5,041,451 - 5,066,613 nt. (total: 25,163 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

EKD16_RS21345: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

EKD16_RS21350: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Hydrolases: hydrolase

EKD16_RS21330: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

EKD16_RS21415: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Lyases: cyclase dehydratase

EKD16_RS21375: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

EKD16_RS21380: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002631.2	0.53	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000560.5	0.47	ribosomal	subtilomycin	Bacillus subtilis
BGC0002635.2	0.46	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0002103.2	0.45	other (other)	pulcherriminic acid	Bacillus subtilis subsp. subtilis str. 168
BGC0002316.2	0.44	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001483.4	0.43	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0001223.5	0.42	other (other)	hydroxysporine	uncultured bacterium
BGC0001727.3	0.42	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000520.5	0.42	ribosomal	lacticin 3147 A1, lacticin 3147 A2	Lactococcus lactis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002631.2	0.53	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000560.5	0.47	ribosomal	subtilomycin	Bacillus subtilis
BGC0002635.2	0.46	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0002103.2	0.45	other (other)	pulcherriminic acid	Bacillus subtilis subsp. subtilis str. 168
BGC0002316.2	0.44	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001483.4	0.43	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0001223.5	0.42	other (other)	hydroxysporine	uncultured bacterium
BGC0001727.3	0.42	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000520.5	0.42	ribosomal	lacticin 3147 A1, lacticin 3147 A2	Lactococcus lactis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EKD16_RS21375

EKD16_RS21370

Leader:

MSTSTIDQPAELQQITDPGNETDPFGLDITFIENTPATE

Core:

TELLCSTGDNCGSSCPSACTTS

Core with dehydrations:

DhbELLCysDhaDhbGDNCysGDhaDhaCysPDhaACysDhbDhbDha

antiSMASH-DB 4.0 matches for EKD16_RS21370

100.0%

Lanthipeptides: EKD16_RS21370_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

TELLCSTGDNCGSSCPSACTTS

1

TELLCSTGDNCGSSCPSACTTS

22

95.5%

NZ_KI866523.1and 2 others:

NZ_VYTZ01000014.1 Lanthipeptides: F5972_RS30195_lanthipeptideNZ_VYTZ01000014.1 Lanthipeptides: F5972_RS30195_lanthipeptide

Lanthipeptides: MPTA5024_RS18085_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

T LLCSTGDNCGSSCPSACTTS

1

TVLLCSTGDNCGSSCPSACTTS

22

90.9%

NZ_CP029042.1:

Lanthipeptides: DDE74_RS17120_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

T L+CSTGDNCGSSCPSACTTS

1

TVLMCSTGDNCGSSCPSACTTS

22

with 176 more sequence matches

Only the first 10 are displayed here.

90.9%

NZ_CP045178.1:

Lanthipeptides: GBW32_RS14380_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

T L+CSTGDNCGSSCPSACTTS

1

TMLMCSTGDNCGSSCPSACTTS

22

86.4%

NZ_BBYG01000011.1and 23 others:

NZ_BBZE01000004.1 Lanthipeptides: AOQ56_RS00075_lanthipeptideNZ_BMUG01000008.1 Lanthipeptides: IE264_RS24565_lanthipeptideNZ_CP013219.1 Lanthipeptides: SHL15_RS30830_lanthipeptideNZ_CP021978.1 Lanthipeptides: CEB94_RS33350_lanthipeptideNZ_CP029342.1 Lanthipeptides: B9S64_RS02555_lanthipeptideNZ_CP030073.1 Lanthipeptides: DN051_RS07240_lanthipeptideNZ_CP041610.2 Lanthipeptides: FNV62_RS40125_lanthipeptideNZ_CP041613.2 Lanthipeptides: FNV68_RS42745_lanthipeptideNZ_CP051006.1 Lanthipeptides: HEP81_RS07390_lanthipeptideNZ_CP074102.1 Lanthipeptides: SMIR_RS04425_lanthipeptideNZ_CP101397.1 Lanthipeptides: NLU04_RS20150_lanthipeptideNZ_CP113910.1 Lanthipeptides: HUV60_RS04250_lanthipeptideNZ_FKJI03000004.1 Lanthipeptides: A7P67_RS09720_lanthipeptideNZ_JACHCX010000047.1 Lanthipeptides: HDC93_RS37275_lanthipeptideNZ_KQ948477.1 Lanthipeptides: AQJ27_RS43755_lanthipeptideNZ_KQ948550.1 Lanthipeptides: AQJ30_RS07460_lanthipeptideNZ_LIVY01000006.1 Lanthipeptides: AMK30_RS21645_lanthipeptideNZ_LMDL01000002.1 Lanthipeptides: ASD08_RS03885_lanthipeptideNZ_RAII01000001.1 Lanthipeptides: EBH12_RS06225_lanthipeptideNZ_SHKS01000004.1 Lanthipeptides: EV567_RS16240_lanthipeptideNZ_SHKU01000001.1 Lanthipeptides: EV284_RS02155_lanthipeptideNZ_SZNQ01000001.1 Lanthipeptides: E4U91_RS28815_lanthipeptideNZ_VIUV01000003.1 Lanthipeptides: FB570_RS08885_lanthipeptide

Lanthipeptides: AOQ37_RS11080_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

T L+CSTGD CGSSCPSACTTS

1

TVLMCSTGDTCGSSCPSACTTS

22

86.4%

NZ_JAHLXF010000013.1:

Lanthipeptides: KQY13_RS21225_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGDNCGSSCPSACTTS

1

LMCSTGDNCGSSCPSACTTS

20

86.4%

NZ_ML123117.1:

Lanthipeptides: EES46_RS09865_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

T L+C TGDNCGSSCPSACTTS

1

TVLMCGTGDNCGSSCPSACTTS

22

81.8%

NZ_BMSO01000039.1and 5 others:

NZ_BMTU01000002.1 Lanthipeptides: IE118_RS05930_lanthipeptideNZ_BMUK01000002.1 Lanthipeptides: IE276_RS05195_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS30040_lanthipeptideNZ_KB907208.1 Lanthipeptides: G339_RS0101560_lanthipeptideNZ_KB907208.1 Lanthipeptides: G339_RS0101560_lanthipeptide

Lanthipeptides: IE184_RS41715_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGD CGSSCPSACTTS

1

LMCSTGDTCGSSCPSACTTS

20

81.8%

NZ_BMVW01000003.1and 2 others:

NZ_CP011799.1 Lanthipeptides: QR97_RS02045_lanthipeptideNZ_JAEVFI010000002.1 Lanthipeptides: JJV70_RS02680_lanthipeptide

Lanthipeptides: IE242_RS12765_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

T L+CSTGD CGSSCPSAC TS

1

TVLMCSTGDTCGSSCPSACVTS

22

81.8%

NZ_CP050976.1:

Lanthipeptides: HEP86_RS36805_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

T L+C+TGD CGSSCPSACTTS

1

TVLMCTTGDTCGSSCPSACTTS

22

81.8%

NZ_FOQY01000053.1:

Lanthipeptides: BM085_RS43985_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+CSTGDNCG+SCPSACTTS

1

LMCSTGDNCGTSCPSACTTS

20

77.3%

NZ_CP070379.1:

Lanthipeptides: JVE63_RS10125_lanthipeptide

1

TELLCSTGDNCGSSCPSACTTS

22

T L C+TGD CGSSCPSAC TS

1

TVLTCTTGDTCGSSCPSACVTS

22

77.3%

NZ_FNCN01000004.1:

Lanthipeptides: BLQ73_RS05220_lanthipeptide

3

LLCSTGDNCGSSCPSACTTS

22

L+C TGD CGSSCPSACTTS

1

LMCGTGDTCGSSCPSACTTS

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.32 out of 24.78

Confidence: medium

Additional weak hits (2):

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.86 out of 27.18

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.61 out of 29.65

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

EKD16_RS21370 - Class I

Cleavage pHMM score: -6.20

RODEO score: 24

Monoisotopic mass: 1989.8 Da

Molecular weight: 1991.1 Da

Number of bridges: 4

Additional modifications: OH

Alternative weights: (assuming N unmodified Ser/Thr residues); 2009.1 Da N = 1; 2027.2 Da N = 2; 2045.2 Da N = 3; 2063.2 Da N = 4; 2081.2 Da N = 5

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 141 - 225; Score: 65.7

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response

NZ_CP036455.1 - Region 14 - T1PKS

Download region SVG

Location: 5,566,607 - 5,614,151 nt. (total: 47,545 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKD16_RS23810: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EKD16_RS23840: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Lyases: dehydratase

EKD16_RS23875: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: racemase

EKD16_RS23885: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002192.3	0.60	PKS	FR901512	fungal sp. No.14919
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.59	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002192.3	0.60	PKS	FR901512	fungal sp. No.14919
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.59	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 29.02 out of 30.11

Confidence: strong

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.61 out of 23.43

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.53 out of 23.43

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.39 out of 29.72

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.57 out of 20.97

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.88 out of 20.97

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.36 out of 20.97

Confidence: medium

Additional weak hits (26):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.35 out of 32.74

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.38 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.48 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.40 out of 31.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.62 out of 36.54

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.66 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.86 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.56 out of 27.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.42 out of 24.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.85 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.97 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.92 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.84 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.10 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.91 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.91 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.04 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.35 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.12 out of 29.38

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.81 out of 33.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.22 out of 24.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.10 out of 28.14

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.43 out of 20.01

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.89 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 21, location 5566606 - 5614151:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKD16_RS23840: pk

PKS_AT (564..859): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Propionyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 93.8
Methoxymalonyl-CoA: 79.4
Malonyl-CoA: 73.3
Ethylmalonyl-CoA: 52.9
Propionyl-CoA: 37.0
Isobutyryl-CoA: 29.8
inactive: 28.3
fatty_acid: 26.1
Benzoyl-CoA: 22.4
trans-1,2-CPDA: 21.6
CHC-CoA: 20.6
2-Methylbutyryl-CoA: 20.6
Acetyl-CoA: 20.4
3-Methylbutyryl-CoA: 8.2