LOCUS       NZ_CP036402            10357 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Egibacter rhizosphaerae strain EGI 80759 chromosome, complete
            genome.
ACCESSION   NZ_CP036402
VERSION     NZ_CP036402.1
KEYWORDS    RefSeq.
SOURCE      Egibacter rhizosphaerae
  ORGANISM  Egibacter rhizosphaerae
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae; Egibacter.
REFERENCE   1  (bases 1 to 4637169)
  AUTHORS   Chen,D.-D., Tian,Y., Jiao,J.-Y., Zhang,X.-T., Zhang,Y.-G., Zhang,Y.,
            Xiao,M., Shu,W.-S. and Li,W.-J.
  TITLE     Egibacter rhizosphaerae EGI 80759T
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4637169)
  AUTHORS   Chen,D.-D., Tian,Y., Jiao,J.-Y., Zhang,X.-T., Zhang,Y.-G., Zhang,Y.,
            Xiao,M., Shu,W.-S. and Li,W.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2019) School of Life Sciences, Sun Yat-Sen
            University, 135 Xingang West Road, Guangzhou 510275, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.52x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004322855.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 04:33:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,252
            CDSs (total)                      :: 4,199
            Genes (coding)                    :: 4,154
            CDSs (with protein)               :: 4,154
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 36 of 45
            Pseudo Genes (internal stop)      :: 3 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:53:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2443281
            Orig. end                         :: 2453638
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036402.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10357
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10357
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10357
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5357](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5357)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(134..679)
                     /locus_tag="ER308_RS11420"
                     /old_locus_tag="ER308_11420"
     CDS             complement(134..679)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /locus_tag="ER308_RS11420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ER308_11420"
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_205745575.1"
                     /transl_table=11
                     /translation="MLPTSRRAVESMLAMAELGQAELVAELGAGTGSHTRAILSQLGAR
                     ARLVAFEIDPAMAATVRERLPDPRLEVVTASAEELEHHLDGQRPQVVVSALPFTSLPAG
                     AGTAILDRTARTLAPGGVLLVLQYSPLITSELTRRFGWVRRKMCLRNVPPAFLFACRDP
                     RPADDGQDAPGLASGPSS"
     misc_feature    complement(636..650)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.6"
                     /tool="antismash"
     misc_feature    813..823
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    866..881
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.85"
                     /tool="antismash"
     gene            1056..1985
                     /locus_tag="ER308_RS11425"
                     /old_locus_tag="ER308_11425"
     CDS             1056..1985
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (12-239). E-value: 8.2e-37.
                     Score: 118.9. Matches aSDomain:
                     nrpspksdomains_ER308_RS11425_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /locus_tag="ER308_RS11425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ER308_11425"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_205745576.1"
                     /sec_met_domain="ADH_N (E-value: 2.9e-09, bitscore: 34.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.2e-11, bitscore:
                     41.9, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAVADDYGAPDVLEVREVDDPKVGPDYALVRVHAASINPVDYK
                     IVEGGLDAAFPVIWPLITGWDVAGEVVGVGPAVRHVEPGQAVFGYARKDFVGAGTWAEQ
                     VAVPARGIAPAPRQLDAVAASCLPLAGMTAWQALVEDLEVGRGDTVLIHAATGGVGHLA
                     TQIALARGARVVGTCREANHDVLRELGGEPVTYGEGLADRVREVAPDGVDAVADFSPQG
                     SLAASDPVLRSPGRVVSVRDPQAAYERGGTYVFVRPDVDHLRSLAELADAGRLAPRVQS
                     VHELADVRAAVAEASEGTVRGKVVLRVE"
     aSDomain        1092..1772
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ER308_RS11425_PKS_ER.1"
                     /evalue="8.20E-37"
                     /label="ER308_RS11425_PKS_ER.1"
                     /locus_tag="ER308_RS11425"
                     /protein_end="239"
                     /protein_start="12"
                     /score="118.9"
                     /tool="antismash"
                     /translation="DVLEVREVDDPKVGPDYALVRVHAASINPVDYKIVEGGLDAAFPV
                     IWPLITGWDVAGEVVGVGPAVRHVEPGQAVFGYARKDFVGAGTWAEQVAVPARGIAPAP
                     RQLDAVAASCLPLAGMTAWQALVEDLEVGRGDTVLIHAATGGVGHLATQIALARGARVV
                     GTCREANHDVLRELGGEPVTYGEGLADRVREVAPDGVDAVADFSPQGSLAASDPVLRSP
                     GRVVS"
     CDS_motif       1494..1556
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ER308_RS11425_0001"
                     /evalue="1.10E-07"
                     /label="PKSI-ER_m5"
                     /locus_tag="ER308_RS11425"
                     /protein_end="167"
                     /protein_start="146"
                     /score="24.2"
                     /tool="antismash"
                     /translation="GDTVLIHAATGGVGHLATQIA"
     gene            complement(2083..2781)
                     /locus_tag="ER308_RS11430"
                     /old_locus_tag="ER308_11430"
     CDS             complement(2083..2781)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="ER308_RS11430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ER308_11430"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_131155111.1"
                     /transl_table=11
                     /translation="MITINGLAKSFGPRTLWSNLDCRVADGEMLAVTGPSGAGKSTLLN
                     CIGLLERPTHGQILCRGADITRFGPRARRRFRRDVLGYLFQSYALIETATVAENLRVTM
                     APKGQRRAVLRDALDHVGLAGHENERIHHLSGGEQQRVAVARLLVKQPSVILADEPTGA
                     LDRANAAVVMDALREMSAAGAAVLIASHTPSIWSRCDAVLELSAPRPVDEQPGASAGSR
                     PGRGVQAART"
     misc_feature    2768..2781
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            complement(2795..4888)
                     /locus_tag="ER308_RS11435"
                     /old_locus_tag="ER308_11435"
     CDS             complement(2795..4888)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ER308_RS11435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ER308_11435"
                     /product="hypothetical protein"
                     /protein_id="WP_131155112.1"
                     /transl_table=11
                     /translation="MVLLRYRTIKLTHLVLLAASSLFAFLSLQDLDDVRVLGSDGDVWI
                     TGSDGSAETAQVARAVSSFARAHGVSVVREEADPAEPDTVTHLYVAAGNPESAPASWLA
                     EGHVPFGHRMDVHVHAFEAISQGDARGVYHVYGSEEAAAALQDELTGLGLSGEVVAPFD
                     LRQRFLYQAGAGAPLGLLFAVVALGAALTVGASVLLGARDYAVLRLHGTSFPRMLWHEA
                     ARVLPFWVFAAAGVVVVTSIVLALYNGFARAGLFLAVAAALAGVLSAVAAVAHVGALVA
                     ASSTKALRALKGGIPTKPSMVGVYLVRLCAALLVLLLSESAVESWQRTSQHEGSQEELA
                     ALGEDATYVALPGSRTRQAAEAMSEEVGEWLRRADERDQIIASYQWPMAQVLPEVGLPE
                     RRVLRVNETFLRQQPILDASGQRHAPDRDNEAVRVLIPESLQDHADAIVERVPELVDPE
                     QAGAPVSDVEPAWSRDGQTVATLQSEQNTNSLGRVLVDDPVVIVVPNGSSVTSPANYTA
                     MATSDGIIFPDRDDALSALGEEVSAEHVAGLAPVGHRAAAEHADAMHERRINVLSLTTA
                     VLVLFVTGLAVAGIYGRHGAQAIFAKHVSGWSFWATHRKLFLLDALVASALVTWVGQSI
                     WATINRVEQFAERGVPPPPNLPQADWWQLAAAAGVAVVATTLLMATTGLIHRRIVAEHA
                     ADA"
     misc_feature    4986..4999
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            complement(5001..5357)
                     /locus_tag="ER308_RS11440"
                     /old_locus_tag="ER308_11440"
     CDS             complement(5001..5357)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="ER308_RS11440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ER308_11440"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_131155113.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 3.5e-13,
                     bitscore: 47.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKSLARNLLAVVAAGAFVIGGAGTAMADSGEVEMDPDSRVAPAD
                     NSELVGGGLWQYGTNWFSGYSNYLHEENCHGSSAQSGDNFQRDHGVPAGLWATADVERD
                     GVNTIRAFWTNSCG"
     gene            complement(5563..5697)
                     /locus_tag="ER308_RS22805"
     CDS             complement(5563..5697)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ER308_RS22805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_276319841.1"
                     /transl_table=11
                     /translation="MVLTPLIEPVSPADGAATQGAPTAVMAEDATSGKGGGNWPFFDD"
     misc_feature    5991..6011
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.46"
                     /tool="antismash"
     gene            6072..7451
                     /locus_tag="ER308_RS11445"
                     /old_locus_tag="ER308_11445"
     CDS             6072..7451
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /locus_tag="ER308_RS11445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ER308_11445"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_131155114.1"
                     /transl_table=11
                     /translation="MQRPGEVGVRGLEQPYLGQRLRRLRIERFLTQRQVAGQAMTASYV
                     SLVEAGRRTPTLDAVVAMAHALGVSLGDLLSVEGLADLGLATGGITEQAPHARDAPLDT
                     LIHEWLADDAMQVRDHATAAAILTGAFDKARDRGDHLAAIATGRRLQGALAATNQHQRR
                     AELLTDLLDLVVDQRSVDLEVLVRSDLAGALRDIDDIDRALACTRAALARLPASSLADT
                     SAYLRLMTVHVSVLVEADDHDELPGALSQLGDAVDKVDSAELRGRAHWVASRGHAVLGN
                     AGHASQHLAQAHALLSPASMPLLEWLRFCRSAAMVALEATDTDTARRWVEDADAAAAAL
                     GVEAERREVDVLRARCLLQAGHAEAARDFLAKALEGTEPAPDAEHAGSLPHLTTQLWLL
                     HAQAAHECGDTTHAAQLLRQAADHAESKGHYETALRALRVRDALNSEPPDTSVLDVSEF
                     L"
     misc_feature    7236..7238
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7399..8571)
                     /locus_tag="ER308_RS11450"
                     /old_locus_tag="ER308_11450"
     CDS             complement(7399..8571)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /locus_tag="ER308_RS11450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ER308_11450"
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="WP_205745577.1"
                     /sec_met_domain="GST_C (E-value: 2.5e-05, bitscore: 22.4,
                     seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEQRGTTATVDDGRFERDTNYLSDRILDDPQARWPVEPSRYRLV
                     VARACPWANRAVIVRRLLGLEDALPMGIAAPTHDWRSWKFDLDPGEVDPVLGIHRLREA
                     FEAREPGYDRGITVPAIVDVPSGQVVTNDFRQITVDLSTQWRAHHRSGAPDLYPEHRRD
                     EIEGVAELVYRDVNNGVYEAGFAGSQAAYEDAYQRLFDRLDWLSDRLADQRYLVGDHIT
                     EADIRLFTTLARFDASTTTTSAATDRSWRSCRSCGPTPATCSRPPDSATPPTSCRSSST
                     TTSCTPASTPRGSCRRAPTCTDGRSPTAARNSAALPSGRARPRRRRRRTSACPPSTTRP
                     CARWPHQPWGEWPTPPTHSSGLTTSIALGVGALLTGTPTHRAPRCQAARS"
     misc_feature    complement(8561..8576)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.6"
                     /tool="antismash"
     gene            complement(8689..9261)
                     /locus_tag="ER308_RS11455"
                     /old_locus_tag="ER308_11455"
     CDS             complement(8689..9261)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /locus_tag="ER308_RS11455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ER308_11455"
                     /product="DUF2269 family protein"
                     /protein_id="WP_165492020.1"
                     /transl_table=11
                     /translation="MIDLTSLFLHVLAAIGLVAGGLAQVMAGSRLRTAESGSELLAWTR
                     FTRTAGWLVAASAALSLLTGGHLAGAVWGGEAGGFANPFITLGLVGLIVLVPIGPAVGE
                     RRFRRLAEAAAELGESTASERLHRAARSPALWGPVHSLVGVGVGQVALMVYKPGWLVGA
                     LVLVLTFAAGWVAGSAVAARSAPAAHG"
     misc_feature    9247..9262
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            complement(9501..9749)
                     /locus_tag="ER308_RS11460"
                     /old_locus_tag="ER308_11460"
     CDS             complement(9501..9749)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ER308_RS11460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ER308_11460"
                     /product="hypothetical protein"
                     /protein_id="WP_205745578.1"
                     /transl_table=11
                     /translation="MSGVQVGTFEQPDETIPVEKATAERINVSGVSVWRFVFEPGWRYT
                     QHFEPDPCTAPHAAYIVSGTLHVAMDDGSTAEGGPGR"
     misc_feature    complement(10084..10098)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 cggaagaacg ccatcacgcc agccgccagc ccgaccaggg gccagccgac gcgccgtccc
       61 gaccggacca gcagggcgcc ggcagccagc gcggggacgg cgtagcgaca cgccagcggc
      121 caggtgcgcc ggctcatgag gagggtccac tggcgaggcc gggtgcatct tgcccgtcgt
      181 cggcgggtcg cggatcgcga caggcgaaga ggaacgcggg cggcacgttg cgcaggcaca
      241 tcttgcgacg cacccagccg aagcggcggg tcagctccga ggtgatcaga ggggagtact
      301 gcaggaccag cagcacaccg cccggggcca gtgtccgcgc cgtgcggtcg aggatcgcgg
      361 tgccggcgcc cgcgggcagc gacgtgaacg gcaacgccga gaccactacc tggggacgtt
      421 gaccgtcgag gtggtgttcc agctcctcgg ccgaggccgt gaccacctcc agtcgggggt
      481 cgggcaaacg ctcgcgcacc gtcgcggcca tcgcggggtc gatctcgaag gccaccaggc
      541 gcgcccgggc ccccagctgc gacaggatgg cccgcgtgtg cgagcccgtg cccgctccga
      601 gctcggccac cagctcggcc tgtccgagct cggccatcgc cagcatggat tccaccgcac
      661 ggcgcgacgt cgggagcacc gctccgactc gtcgggggtg cgcggcgaag gagcgtagga
      721 acagcagctt gtcggacaga tcggacccag cgctcggggt ggttgcactt ccagcgtgac
      781 agggtgccac caggaatctc atcatcgtgc catgccatgc caccgaggtg cagccgcatc
      841 ctggccgacg cgacccgctc accgtctttc gacatggaaa ccggtccggc ctgctcgtgc
      901 ccgctgatcg ttcggggtct cggcagcgct gcgcagaatg ccacctcccc cgagtcgcgc
      961 ctcacggcca cgggccgagt gtcggcagcg ctgggcaggg atgccacccg ctttcagtgg
     1021 ggacgctgcg cccgtgcccg atgaggaggt gccgtgtgcg cgctgccgtc gcggacgact
     1081 acggagctcc cgacgtcctc gaggtccgcg aggtcgacga cccgaaggtg gggccggact
     1141 acgcgctcgt gcgcgtccac gccgcgagca tcaacccggt cgactacaag atcgtggagg
     1201 gcggcctcga cgcggcgttc ccggtgatct ggccgctgat caccgggtgg gacgtcgccg
     1261 gggaggtcgt cggggtgggc cccgccgtgc gccacgtcga gccaggccag gccgtgttcg
     1321 gctacgcccg caaggacttc gtgggcgcgg gcacgtgggc cgagcaggtc gcggtgccgg
     1381 cccgtggaat cgcgcccgcg ccgcggcagc tcgacgcagt cgcggcgagc tgcctgccgc
     1441 tggcgggcat gacggcctgg caggcgctgg tcgaggatct cgaggtcggc cgtggcgaca
     1501 ccgtgctcat ccacgccgcg accggcgggg tgggccacct cgcgacgcag atcgccctcg
     1561 ctcgaggcgc ccgtgtggtc gggacctgcc gcgaggccaa ccacgacgtt cttcgcgagc
     1621 tcgggggcga accggtgacc tacggtgagg ggctcgccga ccgcgttcga gaggtcgcgc
     1681 ccgacggggt cgacgccgtc gcggacttct caccgcaggg gtcgctggcg gccagcgacc
     1741 ccgtgctgcg cagcccgggg cgggtcgtga gcgtccgcga cccgcaggct gcgtacgagc
     1801 gcggcggcac gtacgtgttc gtccgcccgg acgtcgacca cctgcgctcg ctcgccgaac
     1861 tcgccgatgc tggccggctc gcgccccgcg tccagtcggt tcacgagctc gccgacgtca
     1921 gggccgccgt cgccgaggcc tcggagggaa ccgtccgcgg gaaggtcgtc ctgcgcgtcg
     1981 agtagaccag cgcgccgaga cggtctgcgg agcgaacgtt ggcggcgcct cagtcctgac
     2041 ccccagctct tcgcggtact gcagtcgaag aacagcctct ccctaggttc gagccgcctg
     2101 aacgccccgc ccaggccgcg agcccgcgct ggcgccgggt tgctcgtcca cgggccgcgg
     2161 ggccgagagc tcgagtacgg catcgcatcg gctccagatc gagggcgtgt ggctggcgat
     2221 gagcacggcg gcgccggcag cgctcatctc ccgtagcgcg tccatcacga cggcggcgtt
     2281 ggcgcggtcg agggcaccgg tcggctcgtc ggccaggatc accgacggct gcttgaccaa
     2341 cagccgggcg accgcgacgc gctgctgttc accgccgctg aggtgatgga tccgctcgtt
     2401 ctcgtgtccc gccagcccca cgtggtcgag cgcgtcgcgc aagaccgcgc gtcgctgccc
     2461 cttgggagcc atcgtgaccc tgaggttctc ggcgaccgtg gcggtctcga tgagcgcgta
     2521 gctctggaac agatagccta ggacgtcgcg tcggaaccga cgtcgtgctc gcgggccgaa
     2581 gcgggtgatg tcggcgccgc ggcacaggat ctggccgtgg gtgggccgct cgagcaggcc
     2641 gatgcagttg agcagggtcg acttgcccgc tccgctgggg ccggtcacgg ccagcatctc
     2701 accgtcagcg acacggcagt ccaggttgga ccacagtgtg cgcgggccga aagacttcgc
     2761 caggccattg atcgtgatca tggacctcac cccctcaggc gtcggctgcg tgctcggcga
     2821 cgatgcgtcg gtggatgagg cctgtggtgg ccatcaacag ggtcgtcgcg accaccgcga
     2881 cgccggcagc ggcagcgagc tgccaccagt cggcttgtgg gaggttgggc ggcggtggga
     2941 caccgcgctc ggcaaactgc tcgacccggt tgatcgtcgc ccagatgctc tggccgaccc
     3001 acgtcacgag cgcgctcgcg acgagcgcat caagaaggaa cagtttccgg tgggtggccc
     3061 agaaggacca gccgctgacg tgcttggcga agatggcctg ggcgccgtga cggccgtaga
     3121 tgccagccac tgcgagcccg gtgacgaaca gcacgagcac cgccgtcgtc aggctcagca
     3181 cgttgatccg gcgctcgtgc atcgcgtccg catgctcggc ggcggcgcga tggcccaccg
     3241 gggcgaggcc ggcgacgtgc tcagctgaca cctcctcccc aagcgcggac aacgcgtcgt
     3301 cacggtccgg gaagatgatc ccgtcactgg tcgccatcgc cgtgtaattg gctggcgagg
     3361 tcaccgacga cccgttggga accacgatga ccacgggatc gtcgaccagc accctgccga
     3421 gactgttggt gttctgctcg gactggaggg tcgccacggt ctggccatcc ctcgaccacg
     3481 ctggctcgac gtcggacact ggggctcccg cctgctcggg atcgacgagc tccggcacgc
     3541 gctcaacgat ggcgtcggcg tggtcctgca gcgactccgg gatcaggacc cgcaccgcct
     3601 cgttgtcccg atcgggggcg tggcgctgcc cggaggcatc caggatgggc tgctgccgga
     3661 ggaacgtctc gttgacgcgg agcacgcggc gctctggaag gccgacctct ggcaagactt
     3721 gcgccatggg ccactgatac gaggcgatga tctgatcccg ctcgtcggcc cggcggagcc
     3781 actcgccgac ctcctcggac atggcctctg ctgcctgccg cgtgcgactg ccgggcagcg
     3841 ccacgtaggt cgcgtcctct cccagcgccg cgagctcctc ctgactgccc tcatgttgtg
     3901 aggtccgctg ccaggactcc acagcgctct cgctcaacag cagcaccagc agtgccgcgc
     3961 agagccgcac cagatagacg cccaccatgg acggcttggt cgggataccc cccttcaagg
     4021 cacgcagcgc tttcgtgctg ctcgccgcga cgagcgcccc cacatgcgcg accgcggcga
     4081 ccgcgctcaa cacaccggcc agagcggccg cgacggcgag gaacaagcca gcgcgcgcga
     4141 acccgttgta gagagccaac acgatcgacg tcacaaccac cacgccggcg gcggcgaaca
     4201 cccagaacgg cagcacccga gccgcctcat gccacagcat tcgcggaaac gacgtcccat
     4261 gcagacgcag caccgcgtag tccctcgcgc ccaacagcac gctggccccg accgtcaacg
     4321 ctgccccgag ggccaccacc gcgaacagca gcccgagggg agccccggcc ccggcctggt
     4381 acaggaaccg ttgcctgagg tcgaacggcg ccaccacctc tccactgagt cccaggccag
     4441 tgagctcatc ctgcagggcg gcggcggcct cctcggaccc gtagacgtgg tagacgccgc
     4501 gggcatcacc ttggctgatg gcctcgaacg cgtgcacgtg gacgtccatg cggtggccga
     4561 agggaacgtg gccctctgcc agccaggacg ccggcgccga ctcgggattg cctgccgcga
     4621 cataaaggtg cgtcaccgtg tcgggctcag ccgggtcggc ttcctcgcgc accaccgaga
     4681 ccccgtgcgc tcgagcgaat gagctgacag ccctggctac ctgcgcagtc tccgcggagc
     4741 cgtcgctgcc ggtgatccag acgtcgccgt cgctgccgag gacgcggacg tcgtcgaggt
     4801 cttgcaacga caggaacgcg aagaggctcg acgcggcgag caacaccaga tgggtgagct
     4861 tgatggtgcg gtatcgcaga aggaccacct ctctggatag ccgccggcgg ccatggccgc
     4921 aagggtgacg ccagctcgaa caactccgga cgccacccct gcgaccctat cggtcttcgc
     4981 gccgaacgat acagatcaaa tcaaccacaa ctgttcgtcc agaaggcgcg aatggtgttc
     5041 acaccgtccc gctctacgtc ggcggttgcc cagagaccag ccggcacccc atggtcgcgc
     5101 tggaaattgt ctcccgactg cgcggacgat ccgtgacagt tctcctcgtg gagatagttc
     5161 gagtaccccg agaaccagtt cgtgccatac tgccagaggc cgccccccac tagctcgctg
     5221 ttgtccgcgg gggcgaccct gctatcgggg tccatctcga cttcgccgct gtcggccatc
     5281 gcggttccgg cgcccccgat cacaaaagct ccagcagcga cgactgcaag gaggttgcgg
     5341 gcgagtgatt tagacataaa gccctccgat tctcgtccag cttctgctca tggtcgactc
     5401 tcttttgctc agtactcgac gtggtgctgg gcacggacta tgtcatttgc caccccctgt
     5461 gtggattcgt cgatgcggcg gacaagacac cccaatcggc gcctcccatg catcagggct
     5521 gtcggcttct gcggatccgt cgagacatcg aggcgcgggc ggtcagtcgt caaagaaggg
     5581 ccagttgccg ccgcccttgc cggacgtggc gtcctccgcc atgaccgcgg ttggcgctcc
     5641 ctgggtggcc gccccgtccg ccgggctcac gggctcgatc agtggcgtca gcaccatcgc
     5701 gaaggatgag gcggctgccg cgacgcttcg gggaatccgg actgtcatgt catgccctct
     5761 ctcttggcgt gactggcgtt atacctcgag agtctggacg tcgacacaac ccgtgtcaac
     5821 cccgtcagta accgcggtca tctccgtcgc atggaagcct cgcaaccacc gacgtatcgg
     5881 cgcgcaccgt cacccatctg tgtacttgag cagcgactga actcctcgcg tagacgcgac
     5941 gatgtgccgt gtgcatggcg cgacctcacc acagttgtgt ttgtgttcac gaagatgaca
     6001 gaggctaggt tgcggtgtag gcttactgtg gttgtagtgc ggatcggtcc ccatgggtcg
     6061 gagctcttga ggtgcagcgt ccgggtgagg taggcgtcag ggggctcgag cagccctacc
     6121 tgggacagcg gctccggcgg ctgcggatcg aacgcttcct gacgcagcgg caggtcgcgg
     6181 ggcaggccat gaccgccagc tacgtgtcgc tcgtcgaggc ggggcggcgc acgccgacgc
     6241 tcgacgcggt ggtggcgatg gctcatgcgc tcggcgtgtc gctgggtgac ctgctcagcg
     6301 tggaagggct ggccgacctt ggcctggcca cggggggaat cactgaacag gccccgcacg
     6361 cccgcgacgc cccgctggac acgctcatcc acgagtggct ggctgacgac gccatgcagg
     6421 tgcgcgacca tgcgaccgcg gcggccatat tgacgggggc cttcgacaag gcccgcgacc
     6481 gcggcgacca cctggcggcg atcgcgacgg gccgacgtct ccagggggcg ctggccgcta
     6541 ccaaccagca tcagcggcgc gcagagctgc tgaccgacct gctcgacctc gtcgtcgacc
     6601 agcgctcggt ggacctggag gtgctggtgc gcagcgacct cgccggcgcg ttgcgcgaca
     6661 tcgacgacat cgaccgagcg ttggcgtgca ctcgtgcggc cctggcccgg ctgccggcct
     6721 cgtcgctggc cgacacctcg gcgtacctgc gcttgatgac ggtgcacgtg tcggtcctgg
     6781 tcgaggccga cgaccacgac gaattgccag gcgcgctgtc ccaactgggc gacgccgtcg
     6841 acaaggtcga cagcgcggag ctgaggggcc gcgcccactg ggtcgccagc cgcggccatg
     6901 ccgtgctggg caacgccggc catgccagcc agcatctggc gcaggcgcat gccttgctgt
     6961 cgcccgcctc catgccgctg cttgagtggc tgcggttctg tcgctccgcg gcgatggtcg
     7021 cgctcgaggc caccgacacc gacacggcgc ggcgatgggt tgaggacgcg gacgctgccg
     7081 ccgcggcact gggcgtcgag gcggagcgtc gcgaggtcga cgtgctcagg gcccggtgcc
     7141 tgctgcaggc cggacacgcc gaagcggccc gcgacttcct cgccaaggcg ctcgagggca
     7201 ccgagcccgc gccggacgcg gagcacgccg ggtcgttacc ccacctgacc acacagttgt
     7261 ggctcctcca cgcgcaggcc gcccacgagt gcggtgacac gacccacgct gcccaactgc
     7321 tgcgccaagc cgccgaccac gcggagtcca aaggccacta cgagacggca ctgcgcgcgc
     7381 ttcgcgtccg cgacgcgctc aactccgagc cgcctgacac ctcggtgctc gatgtgtcgg
     7441 agttcctgtg aggagggcgc cgaccccgag ggcaatcgag gtggttaggc cactcgagtg
     7501 cgtgggcggc gtcggccact caccccacgg ctgatggggc caccgcgcgc agggccgggt
     7561 tgtgctcggc gggcacgcgc tcgtcctccg gcggcgccgg cgggggcgtg ccctccccga
     7621 agggagtgcc gccgagttcc tcgcggccgt ggggctgcgc ccatccgtgc aggtcggggc
     7681 ccgccggcac gatccccgag gggttgaggc cggtgtgcac gaggtagtag tgctgcttga
     7741 tctgcacgaa gtcggtggtg tcgccgaatc cgggggtctg gaacaggtcg cgggcgtagg
     7801 cccacaggac cggcagctcc gccagcttcg atcggttgca gcggaagtgg ttgtggtaga
     7861 cgcgtcgaag cgcgcgaggg tcgtgaacag ccggatgtcg gcctcggtga tgtggtcgcc
     7921 gacgaggtac cgctggtcgg ccaggcgatc cgagagccag tcgaggcggt cgaacagccg
     7981 ttggtaggcg tcctcgtagg cggcctgact gccggcgaac cccgcctcgt acacaccgtt
     8041 gttgacgtcg cgataaacca actccgccac gccctcgatc tcgtcccggc ggtgctcggg
     8101 gtacagatcg ggcgcgcccg accggtggtg ggcgcgccac tgggtcgaca ggtccacggt
     8161 gatctggcgg aagtcgttcg tgacgacctg cccggagggg acgtcgacga tggcgggcac
     8221 ggtgatgccc cggtcgtagc cgggttcacg cgcctcgaag gcctcgcgca gccggtggat
     8281 gccgaggacc ggatcgacct ccccgggatc gaggtcgaac ttccagctgc gccagtcgtg
     8341 ggtgggggcg gcgatgccca tcggcagtgc gtcctccagc cccagcaggc ggcgcacgat
     8401 caccgcgcgg ttggcccacg ggcacgcgcg tgccaccacg agccggtagc ggctgggctc
     8461 gaccggccac cgcgcctggg ggtcgtcgag gatgcggtcg ctgaggtagt tcgtgtcgcg
     8521 ctcgaagcga ccgtcgtcga ccgtggcggt cgtgccgcgt tgttcgctca tcgcctcgag
     8581 cctagccgtc ggcggactcg accatgcatg ccgacccacc atgaccgtga ccgcgcctgg
     8641 cctgctgccg ggcacaggag cgcgccacga gcgccgagtc cacggtgttc atccgtgagc
     8701 agcgggagcg gaccgcgcgg cgacggcgct gccggctacc cacccggccg cgaacgtcag
     8761 caccagcacg agggcgccca cgagccaccc cggcttgtag accatcaggg cgacctggcc
     8821 gacgccgacc ccgacgagcg agtggacggg cccccagagc gcggggctgc gggcggctcg
     8881 gtgcaaccgc tcggaagcgg tcgactcccc gagttcggcg gccgcctcgg cgagccggcg
     8941 gaatcgtcgc tcgccgacgg cgggaccgat gggcacgagc acgatcaggc cgacgaggcc
     9001 gagggtgatg aacggattcg cgaacccgcc ggcctcgcca ccccagaccg cgccggcgag
     9061 gtgcccgccg gtcagcagcg acagggctgc ggaggccgcg acgagccacc ccgccgtgcg
     9121 ggtgaagcgc gtccaggcga ggagctcgct cccgctttcg gcggtgcgca gacgtgaccc
     9181 ggccatcacc tgggcgagtc ccccggccac gaggccgatg gccgccagca cgtgcaggaa
     9241 cagcgaggtg aggtcgatca cggggtcctc caagcgcctg gacacgctcc ggacggggaa
     9301 ggacgggcgc gccggcttcc cgatccgagc aggttcgcgc cgagcctacc ggggcgcgga
     9361 ccacccgacc gcctggacgc cggggcgcgg acctcgggct cagccgtcca gcgccgacca
     9421 gtcgacgctc tgggcgaagt cgatgaacac gcagggttcg tcaccgaccg tccacgcgtc
     9481 gtgccccggc gcgatgacca tcacctcccc gggccgccct ccgccgtgct gccgtcgtcc
     9541 atcgccacgt gcaaggtgcc ggagacgatg taggccgcgt gcggagccgt gcacgggtct
     9601 ggctcgaagt gctgcgtgta ccgccagccc ggttcgaaca cgaaccgcca cacgctgacg
     9661 ccgctgacgt tgatccgctc cgccgtcgcc ttctcgacag gaatggtctc gtctggttgc
     9721 tcgaacgtgc cgacctgcac cccgctcatc tgcgcctcct caccgaactc gttcgccccg
     9781 gccacccgtg gtcttgcccg ggtggccctc gcgggagtgt cgcagcctct ccccggttga
     9841 ccagggtcgc acggggctct ggtgccccgg gcccctgtcg gcccggatgg cgcaccgggg
     9901 cgtcggtggc gggtccaaga gcgtcaggcc cttaccgccg ccgcatgggg cgggccagct
     9961 gctggagagg aggcgaggaa gccgatcagc tcctcggcga ccgcgtgggg ctcgatgacg
    10021 ggggcaccgt gtccggcacc ggcgatctcg cggacctcgc tctcggcggt gtgtgccgcg
    10081 acgtggccga ccgcgtcggt gaaccagcgc aggggggtgc ggctgccgtg cagcagcagg
    10141 accggggcgg tgatctcggc gagggccgcg ggactggtcg ggctgaacgc ctcgggctgg
    10201 gactcgagct ggatctcctg cagcagggtc ggggcgtacg tcgcgacgtc ttcgacgtaa
    10261 cccgcggcct ccaacgtggc catctcttcg tcgttggctg gcaggtagca ggtgaatcgg
    10321 tcggtcaggt gcggcagcaa cgcggcccag tcgaggt
//
