LOCUS       NZ_SIJR01000006        20639 bp    DNA     linear   CON 04-JUN-2024
DEFINITION  Rhizobium leguminosarum strain SM4 map unlocalized plasmid pSM4_Rh07
            p_SM4_Rh07, whole genome shotgun sequence.
ACCESSION   NZ_SIJR01000006
VERSION     NZ_SIJR01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhizobium leguminosarum
  ORGANISM  Rhizobium leguminosarum
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
REFERENCE   1  (bases 1 to 196965)
  AUTHORS   Cavassim,M.I.A., Moeskjaer,S., Moslemi,C., Fields,B., Bachmann,A.,
            Vilhjalmsson,B., Schierup,M.H., Young,J.P.W. and Andersen,S.U.
  TITLE     The genomic architecture of introgression among sibling species of
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 196965)
  AUTHORS   Cavassim,M.I.A. and Young,J.P.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2019) Biology, University of York, Heslington,
            York YO10 5DD, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.7; Jigome v. 1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCF_000023185.1
            Genome Coverage                   :: 39.9959x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004307035.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 04:40:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,389
            CDSs (total)                      :: 7,324
            Genes (coding)                    :: 7,141
            CDSs (with protein)               :: 7,141
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 89 of 183
            Pseudo Genes (incomplete)         :: 135 of 183
            Pseudo Genes (internal stop)      :: 23 of 183
            Pseudo Genes (multiple problems)  :: 56 of 183
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 100124
            Orig. end                         :: 120763
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SIJR01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20639
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="1"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20639
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20639
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10639](+)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10639
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     gene            complement(331..1470)
                     /locus_tag="ELG62_RS36275"
                     /old_locus_tag="ELG62_36280"
     CDS             complement(331..1470)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518564.1"
                     /locus_tag="ELG62_RS36275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36280"
                     /product="RHE_PE00001 family protein"
                     /protein_id="WP_130854263.1"
                     /transl_table=11
                     /translation="MISMAYNLAKIGMTALMRPAFDAAVALTRLDERIARSSVGQGWIE
                     RSQFTDACASLWIDGELVHLEDLVLHDATKDIRTPTHELTIAGDVLRTRRRIAGQSPDW
                     ALSADGIRSLRKTSEITAADTDEAATADVIRRPVTTDPEGEGEDVEDIENLPGVDYAAI
                     DAVLARSEAAIENATGPGRARAAEKDPMIYDLDWDEDSRLDEWRGVLRQAENLPAVLQA
                     IVALDAWNELSVLQHAPWLGRLLCASILRQVGITTGANLAAINLGLKTIPVDRRRHRDR
                     ETRLLAIAHGFLAAAEIGMKEHDRLALARTLMERKLDGRRASSKLPELVDMVMAKPLVS
                     AGMVAKTLEVTPQAARRIVLELGLREMTGRGRFRAWGML"
     gene            1661..2812
                     /locus_tag="ELG62_RS36280"
                     /old_locus_tag="ELG62_36285"
     CDS             1661..2812
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046788.1"
                     /locus_tag="ELG62_RS36280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36285"
                     /product="site-specific integrase"
                     /protein_id="WP_130784871.1"
                     /transl_table=11
                     /translation="MAKPKTSLTAVERRAEELDTIAAVLPLERRDELAELLTDHDVETL
                     RHLVNQGMGDNTLRALTSDLAYLEAWGLAATGQSLPWPAPEALLLKFVAHHLWDPQHRE
                     TDPDHGMPADVDEDLRSQGFLRSAGPHAPSTVRRRLANWSTLSKWRGFEGAFASPALKS
                     AIRLAVRAVPRTRRRKSAKAVTGDVLAKLLATCTTDSLRDLRDRAILMVAFASGGRRRS
                     EIAGLRSEQLTVEPPIPGDDGPSLPSLAIHLGRTKTTSGDEDDVVYLTGRPVDALNAWM
                     VAAKIESGSVFRAIGRWGTVSKRAIDPQSVNAIIKQRVELAGLDREEFSAHGLRSGYLT
                     EAANRGIPLPEAMEQSLHRSVQQASSYYNSATRREGQASRLLA"
     gene            2831..3382
                     /locus_tag="ELG62_RS36285"
                     /old_locus_tag="ELG62_36290"
     CDS             2831..3382
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /locus_tag="ELG62_RS36285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36290"
                     /product="DUF418 domain-containing protein"
                     /protein_id="WP_245502829.1"
                     /transl_table=11
                     /translation="MAIARLRLNTQAMHWRLGLAGIALAMLGYGVSWLALLFLPDVPTA
                     LRESPGLLWDLDCPWSLSVAAPHSETTFSILGNTGCAMIVLAACWLAMDSLPRLRKMFW
                     PVVAVGSMSLTVYVLHTVGIAYLLRVMGIDSAGEDGSLSMLLSFVVATSTFAVLWLRAF
                     QRGPMEVLMGRITDLARHIL"
     gene            complement(3702..4091)
                     /locus_tag="ELG62_RS36290"
                     /old_locus_tag="ELG62_36295"
     CDS             complement(3702..4091)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ELG62_RS36290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ELG62_36295"
                     /product="hypothetical protein"
                     /protein_id="WP_130784873.1"
                     /transl_table=11
                     /translation="MCRIYPTWAVTTFVALLFSANTINIGGDLAAMGEAAELVPGPGRQ
                     VFPLLVAVSSRILQVVLSLFTGPASRFRTVIPQLALTEAKALFGTTNIRAIQPGYHRHM
                     AARHACTWQDRQPTPYAIRRWKTGL"
     gene            complement(4706..6010)
                     /gene="repC"
                     /locus_tag="ELG62_RS36295"
                     /old_locus_tag="ELG62_36300"
     CDS             complement(4706..6010)
                     /codon_start=1
                     /gene="repC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003595757.1"
                     /locus_tag="ELG62_RS36295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36300"
                     /product="plasmid replication protein RepC"
                     /protein_id="WP_130854264.1"
                     /transl_table=11
                     /translation="MHTGSVTPFGRRSMTLGMLANQVMAREIKPDQSVDKWKLFRALCE
                     AKALLGIGDRALAVLNSLLTFYPKNELAQENGLVVFPSNIQLSLRTHGMAEQTVRRHLA
                     ALVEAGLLVRKDSPNGKRYVRRDSAGEIDEAFGFSLAPLLARAEEIEQLAAAVMAERLH
                     VQRLRERITLCRRDIAKLIEAAVEEEIPGDWQGLYREFRDLIEGLPRSPTTAQLELLLD
                     ELTALRTDILNRLENQVKSTKQRGNANYIERHIQNSNPESISELEPSFETKQGATAEPD
                     NDRGSLTAAKGRGETGHPFAQKEEEQSRRPGVRNDGGGLKSFPLGLVLQACPEILAYGP
                     DGLIRNWRDLMAAAVIVRSMLGVSPSAYEEAANVMGPENAATVMACILERGGHINSAGG
                     YLRGLTRRSEKGEFAIGPMLMALLKANGQGRLKVG"
     gene            complement(6166..7188)
                     /gene="repB"
                     /locus_tag="ELG62_RS36300"
                     /old_locus_tag="ELG62_36305"
     CDS             complement(6166..7188)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene="repB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009669.1"
                     /locus_tag="ELG62_RS36300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36305"
                     /product="plasmid partitioning protein RepB"
                     /protein_id="WP_128408794.1"
                     /transl_table=11
                     /translation="MSRKHILGVSTDAPETSSADYRAAKNRSMPLLGVARKERDPATKL
                     TANIGNALREQNDRLSRAEEIERRLAEGQVVIELDASSIEPSFVQDRMPGDIEGLVASI
                     REQGQQVPILVRPHPDQPARYQIAFGHRRLRAVSELGIPVKAVVRELTDEQLVVAQGQE
                     NNQREDLTFIEKARFAHHLNRQFSREIVIAAMSIDKSNLSKMLLLVDALPSELIDAIGA
                     APGVGRPSWQQLAELIEKAPSPADVSKYAMSEEVQALPSAERFKAVILSLKPSRIARGL
                     PEVMATPDGTRIAQVMQSKVKLEITIDRKAMPDFAKFVLEHVPALYQAYKVENQLKHGE
                     "
     gene            complement(7185..8399)
                     /gene="repA"
                     /locus_tag="ELG62_RS36305"
                     /old_locus_tag="ELG62_36310"
     CDS             complement(7185..8399)
                     /codon_start=1
                     /gene="repA"
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538730.1"
                     /locus_tag="ELG62_RS36305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36310"
                     /product="plasmid partitioning protein RepA"
                     /protein_id="WP_130784921.1"
                     /transl_table=11
                     /translation="MAKAAEKEVPVIEGLTALMERHANALSSQLQAHHLKVFPPHSEKG
                     IRTFGPSEASKLLGVGESYLRQTASEMPELNLSMSPGGRRMFSIEDIHAIRKHMDRVGR
                     GNRRYLPHRRNGERLQVISVMNFKGGSGKTTTAAHLAQYLAMRGYRVLAIDLDPQASLS
                     ALFGSQPETDVGPNQTLYGAIRYDEEQVPIEQVVRATYIPDLHLIPGNLELMEFEHDTP
                     RALMKRKEGDTLFYGRISQVIEDIADNYDVVVIDCPPQLGYLTLSALTAATSILVTVHP
                     QMLDVMSMNQFLAMTSNLLREIENAGAEFKFNWMRYLITRFEPSDGPQNQMVGYLRSIF
                     GENVLNFPMLKTTAVSDAGLTNQTLFEIERGQFTRSTYDRALEAMNAVNDEIETLIKKA
                     WGRPT"
     misc_feature    8501..8518
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            complement(9311..9523)
                     /locus_tag="ELG62_RS38380"
     CDS             complement(9311..9523)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ELG62_RS38380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_245458276.1"
                     /transl_table=11
                     /translation="MFAPAVVVPPEKPEPKKARQALSPRKASPEAAINLQIDGATLRIA
                     SGTDDATIVSRYPGVEGSMLVRRVW"
     gene            10001..10639
                     /locus_tag="ELG62_RS36320"
                     /old_locus_tag="ELG62_36325"
     CDS             10001..10639
                     /EC_number="2.3.1.184"
                     /GO_function="GO:0061579 - N-acyl homoserine lactone
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482021.1"
                     /locus_tag="ELG62_RS36320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36325"
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="WP_063473646.1"
                     /sec_met_domain="Autoind_synth (E-value: 2.6e-53, bitscore:
                     173.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQILALSTPRTTLEAHLLHIHYQLRARIFSDRLGWEVDVMAGCES
                     DRFDALRPTYVLAVAETGELAGCARLLPALGPTMVADVFPSLLPDGQLKGHAAMVESSR
                     FCVDTRITEGRGDGSVHEATLTMFAGIIEWCIASGYTEIVTVTDLRFERILARVGWPLQ
                     RLGEPKKIGVTMAVAGTLAADADTFLRLCPSNYHSEFAPCQPGGLRRNP"
     gene            10636..11613
                     /gene="trbB"
                     /locus_tag="ELG62_RS36325"
                     /old_locus_tag="ELG62_36330"
     CDS             10636..11613
                     /codon_start=1
                     /gene="trbB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004129936.1"
                     /locus_tag="ELG62_RS36325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36330"
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="WP_130784875.1"
                     /transl_table=11
                     /translation="MNQLRSHPRLVRKLQEALGDQLCVALDDANVVEIMLNPDGRLFIE
                     RLGHGVAPAGEMSSAAAEMVIGTVAHALQSEVGTEQPIISGELPIGGHRFEGLLPPVVT
                     KPAFIIRRRASRLIPLEDYVRTGVMTEYHAAAIRSAISTRLNIIISGGTGSGKTTLANA
                     VIDEIVKSAPEDRLVILEDTAEIQCAAENAVLLHTSDSIDMARLLKSTMRLRPDRIVVG
                     EVRDGAALTLLKAWNTGHPGGVATIHSNTAMSALRRLEQLTAEASQQPMHEVIGEAVDL
                     VISIERTPRGRLVRDIIQVERFINGRYEIESDQFTEEQEARHVA"
     gene            11603..11986
                     /locus_tag="ELG62_RS36330"
                     /old_locus_tag="ELG62_36335"
     CDS             11603..11986
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014858032.1"
                     /locus_tag="ELG62_RS36330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36335"
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_128408797.1"
                     /transl_table=11
                     /translation="MSREHTLIAAALWAAPIVFASVAPALASSGGSLPWEGPLQQIQES
                     ITGPVAGAIALAAVAIAGGMLIFGGELNDFARRLVYVVLVAGILLGATNIVGLFGATGA
                     SIGLTDAQITSIGANGGGEGDHG"
     gene            11979..12278
                     /locus_tag="ELG62_RS36335"
                     /old_locus_tag="ELG62_36340"
     CDS             11979..12278
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984785.1"
                     /locus_tag="ELG62_RS36335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36340"
                     /product="conjugal transfer protein TrbD"
                     /protein_id="WP_027681723.1"
                     /transl_table=11
                     /translation="MAESLSGLRRNRIHRALSRPNLLMGADRELVLITGLAAVILIFVV
                     LTAYSALFGVAVWVVIVGLLRMMAKSDPLMRQVYIRHISYKPYYKATTSPWRRY"
     misc_feature    12279..12288
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            12289..14745
                     /locus_tag="ELG62_RS36340"
                     /old_locus_tag="ELG62_36345"
     CDS             12289..14745
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048158.1"
                     /locus_tag="ELG62_RS36340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36345"
                     /product="conjugal transfer protein TrbE"
                     /protein_id="WP_130788633.1"
                     /transl_table=11
                     /translation="MVALKRFRVTGPSFADLVPYAGLVDNGVLLLKDGSLMAGWYFAGP
                     DSESATDLERNELSRQINAVLSRLGSGWMIQVEAIRIPTVDYPSEDRCHFPDPVTLAID
                     AERRAHFAREQGHFESKHALVLTYRPLESKKTALSKYIYSDEESRKKSYADTVLFVFKN
                     AVRELEQYFANTLSIRRMETRETVERGGERIARYDELLQFARFCTTGESHPIRLPDVPM
                     YLDWIATAELEHGLTPKVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFIFL
                     DAEEARQKLERTRKKWQQKVRPFFDQIFQTQSRSVDQDAMSMVVETEDAIAQASSQLVA
                     YGYYTPVVVLFDSDREALQEKAEAVRRLIQAEGFGARIETLNATDAYLGSLPGNWYCNI
                     REPLINTSNLADLIPLNSVWSGSPISPCPFYPPNAPPLMQVASGSTAFRLNLHVDDVGH
                     TLIFGPTGSGKSTLLALIAAQFRRYENAQIFAFDKGSSLLPLTLAAGGDHYEIGGDNAE
                     EGRALAFCPLSELKSDADRAWATEWIEMLVGLQGVTITPDHRNAISRQIGLMASASGRS
                     LSDFVSGVQLREIKDALHHYTVDGPMGQLLDAEEDGLTLGAFQTFEIEQLMNMGERNLV
                     PVLTYLFRRIEKRLDGSPSLIVLDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQS
                     ISDAERSGIIDVLKESCPTKICLPNGAARESGTREFYERIGFNERQIEIVATAMPKREY
                     YVATSEGRRLFNMSLGPVALSFVGASGKEDLKRIRALKSEHGRDWPIHWLETRGVHDAA
                     SLLKYV"
     gene            14717..15520
                     /gene="trbJ"
                     /locus_tag="ELG62_RS36345"
                     /old_locus_tag="ELG62_36350"
     CDS             14717..15520
                     /GO_process="GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trbJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018068565.1"
                     /locus_tag="ELG62_RS36345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36350"
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="WP_128408799.1"
                     /transl_table=11
                     /translation="MPHRYSSTYRWFACLAAATLTIVTAGPVQAGTATGAATEWTQLAN
                     NAQLVDLMKSSGIQVDNQLTQISQLAEQIQNQLKIYENMVQNTAQLPDHVWGQVESDLN
                     QLRSIVDQGQGIAFSMGNADDVLQQRFQSYADLKTNLPDNATFSSTYQSWSNTNRDTIA
                     SSLKAASLTADQFDSEEDTMSSLRSMSETADGQMKALQVGHEIAAQQVAQMQKLRGLVS
                     QQMTMMGTWLQTEQTDKDLAQARREKFFNAEVKSVPEGQKMEPRW"
     gene            15517..15717
                     /gene="trbK"
                     /locus_tag="ELG62_RS36350"
                     /old_locus_tag="ELG62_36355"
     CDS             15517..15717
                     /GO_process="GO:0009292 - horizontal gene transfer
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trbK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970259.1"
                     /locus_tag="ELG62_RS36350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36355"
                     /product="entry exclusion protein TrbK"
                     /protein_id="WP_128408800.1"
                     /transl_table=11
                     /translation="MSRSVLIALLLAVAAASSAATVLIVNSRNTGIPALTEEQRAVREK
                     FFGSDKDLPPIKEGQEMRPRW"
     gene            15711..16892
                     /gene="trbL"
                     /locus_tag="ELG62_RS36355"
                     /old_locus_tag="ELG62_36360"
     CDS             15711..16892
                     /GO_process="GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trbL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097607.1"
                     /locus_tag="ELG62_RS36355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36360"
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="WP_130784876.1"
                     /transl_table=11
                     /translation="MVNVTVARSFLITGLFFLAYAVPAFAQEGQVLTELENQVSSAAKG
                     WETTIMEAAKSLFWILATIEIGIAAVWLAIQSASLDSWFAELVRRIMFIGFFAFVLSQG
                     PTFARAVVDSLFQIGAGAGSASPAEVFDAGIRVASQMSEQAQFGVFEDNALAIAAVLAM
                     GIVVICLSLVAAIFVSVMVEMYVGLLAGMIMLGLGGSSFTKDFAIRYLVYAFGVGMKLM
                     ALVMIAKIGSNVLLGLAQAPTASSDQFVTTLAIAGISVVVFIIAMYVPNIIQGVVQGAS
                     VSGGMEAIRHGGQVASFAAGAGFLAAGAAGAGFAAAQAARAGGSSVAGAALRGVGASFS
                     SGAQAAGAAAKEKAIGSPGAYAGSILGLANAKLDEQRAGHSGPKPPPERNDKP"
     gene            16913..17575
                     /locus_tag="ELG62_RS36360"
                     /old_locus_tag="ELG62_36365"
     CDS             16913..17575
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482029.1"
                     /locus_tag="ELG62_RS36360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36365"
                     /product="conjugal transfer protein TrbF"
                     /protein_id="WP_128408802.1"
                     /transl_table=11
                     /translation="MAANRAPENPYLAARQEWSERYGSYVKAAAAWRIVGVLSLVMAVI
                     GFSYAMYLSTQVRLVPYIVEVDKLGTAVTAGFPEQIEYADVRVVRATLGNFVTSFRSIT
                     PDAVVQKQYIDRTYALLRTSDPSTEKVNAWFRGNSPFEKAKTSTVAIEVNNIVALSNQT
                     YQIDWTEYERDRKGKETGTRRFRGIATVTLTAPQDEATIRLNPIGLYVRDFDWTAQL"
     misc_feature    complement(17588..17601)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.7"
                     /tool="antismash"
     misc_feature    complement(17593..17608)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.3"
                     /tool="antismash"
     gene            17593..18405
                     /gene="trbG"
                     /locus_tag="ELG62_RS36365"
                     /old_locus_tag="ELG62_36370"
     CDS             17593..18405
                     /GO_process="GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trbG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427455.1"
                     /locus_tag="ELG62_RS36365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36370"
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="WP_130784877.1"
                     /transl_table=11
                     /translation="MKKTGLIAAAGCIAGLLLAAGAQAQSMTSNEVKGTNLSRKWRGTP
                     GLVTTGPDGKVIFLFGETQPSVVCSPLQVCDIELQGGEIVRDVLVGDTVRWKVEPATSG
                     AAGGQAIHLIVKPSEPGLLTSMVVTTSRRTYHIQLKSHPSQYMARIGFEYPEDVSTKLA
                     DINARLETGCIPGTAPDKLNFSYSISGGASWKPKRVYSDGVKTYIQFPKSISGQDAPVL
                     FVVSGGQNRIVNYRMKNDMMTIDYAIDRAILVSGVGWRQQKITLRRGG"
     gene            18409..18849
                     /gene="trbH"
                     /locus_tag="ELG62_RS36370"
                     /old_locus_tag="ELG62_36375"
     CDS             18409..18849
                     /codon_start=1
                     /gene="trbH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048152.1"
                     /locus_tag="ELG62_RS36370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36375"
                     /product="conjugal transfer protein TrbH"
                     /protein_id="WP_128408804.1"
                     /transl_table=11
                     /translation="MRKLFAFFIAVALLSSCQTADDALTTSSTPVAVTGPAASAIAGDM
                     ASRLAEQIGPAGATTTIKMETDPSEFATALKAALKGWGYTVVTDGKVAKDVKPVELAYA
                     IEGFDGEVLVQVTTPSIALGRAYTPTAAGATPASPLSIMQRN"
     gene            18861..20159
                     /gene="trbI"
                     /locus_tag="ELG62_RS36375"
                     /old_locus_tag="ELG62_36380"
     CDS             18861..20159
                     /codon_start=1
                     /gene="trbI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018516723.1"
                     /locus_tag="ELG62_RS36375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_36380"
                     /product="IncP-type conjugal transfer protein TrbI"
                     /protein_id="WP_130784878.1"
                     /transl_table=11
                     /translation="MVQSLQLGTSSQADDHQGMRRLNRLPIIVAIIFIVLFVGVVVIGL
                     SLRGLSFNRGDIESASNSPATSFGDQLKRGVTDGIIGDPEKQEVFQPTPLVMEKEEKHG
                     PVVERRPEERQDRRQRLESEEEWKARLKREQDEQYIREAQRQRMARLQARSTALDSPLK
                     VDMSDVEKSANSTNDTSRQPTNVAANSASDLFAGAMKSGLMGLNVDQNGQTSKEDFFNQ
                     DIKDLGYLPNQVVRQISPYELKRGSVIPATLITGLNSDLPGRITAQVSQNVFDSTTGYW
                     LLIPQGAKLFGRYDSKVLFGQERVLVVWTDLIFPNGSTLQIGGMAGTDAEGYGGFKDEV
                     DRHLWRTFGSAALVAIIGTGIDMSMPESSTLATQDTASDAAQRKFAESFGRVAEETISK
                     NLNVPPTVRIRPGYKFNVMVDQDIVFPSVYGDR"
ORIGIN
        1 agatccgtac tcataaatca atgcggaaag gatgatactg tcttgcggat cgagctgcgt
       61 tgcctgccga tacgagatct catccttggg gacgatgaaa cgccggtggg acccgtgcgg
      121 caagtcctta agctgtctac ttgccaccag atcagcaaac tcgctcgagc gctcgcatag
      181 tgcatcaatt tcaaggaggc gcgggaatag atcaccatcg gcgtgctctg caatgaactt
      241 tatcgcccac tctatcgacg gctttgaaag cgacatgtac ctgtccttgc tacatagaag
      301 atcactatta cagcgaagtt tgactttccg tcaaagcata ccccacgccc gaaacctccc
      361 cctccccgtc atctccctca agccgagttc caaaacgatc cgccgcgccg cctgcggcgt
      421 cacttcgagc gtcttggcca ccattccggc cgacacgagc ggttttgcca tcaccatatc
      481 aaccagctcc ggcagttttg acgaggcccg gcgtccgtcc agctttcgct ccatcagtgt
      541 tcgagccagc gccagcctgt catgctcctt catcccgatc tcggcagccg ccaaaaaacc
      601 gtgggcgatg gcgagcagtc gggtctcgcg gtctcgatgc cggcgccgat cgacaggaat
      661 ggttttcagg ccgagattga tcgcagccag attggcgccc gtggtgatgc cgacctggcg
      721 caggatcgaa gcgcacaaca gcctgccaag ccagggcgcg tgctgaagga cggaaagctc
      781 gttccaggca tcgagggcga caatcgcctg cagcactgcc ggcaggtttt ccgcctgtcg
      841 cagcacgccg cgccattcgt cgagcctgga atcctcgtcc cagtcgagat cgtaaatcat
      901 tggatctttt tcggcagcac gggcgcgacc gggccctgtg gcgttctcga tcgccgcctc
      961 cgatcgggcg agcactgcat cgatggcggc atagtcgacg cccggaagat tttcgatgtc
     1021 ctcgacatct tccccctccc cttccggatc ggtcgtgacc gggcgccgga tgacgtcggc
     1081 ggtcgccgcc tcgtctgtgt cggccgccgt gatctcggag gttttccgca gcgagcggat
     1141 gccgtcggca gagagcgccc aatcggggga ttggccggcg atccgccgac gcgtgcgcag
     1201 gacgtcgccc gcgattgtca gctcatgggt gggcgtgcga atatccttcg tggcgtcgtg
     1261 gaggacgagg tcttcgagat ggacgagttc gccgtcgatc cacagtgagg cgcaggcgtc
     1321 ggtgaattgg gatcgttcga tccatccctg gccgaccgac gagcgggcga tccgctcgtc
     1381 cagacgtgta agcgcgacag ccgcgtcgaa agccggccgc atcagggctg tcatgccgat
     1441 tttcgcgaga ttgtaagcca ttgaaatcat ggtaaatgat ttgttatagg aactctatat
     1501 gaatattgcg gttcctcaca tggtatgatt gagcgttggt gcgctaacca tcgataagta
     1561 gcgtttatcg atggtagatt gatttcaggg tgcaaatcac cgagaatcgc agcagatcac
     1621 ggtcctggag accgctgaaa cccgctattg tggagcctcc gtggccaaac ccaagacatc
     1681 actgaccgcc gtcgagcggc gcgccgaaga gctcgacacc atcgccgccg tgctgccgct
     1741 ggaacgccgc gacgaactcg ccgaactgct caccgaccac gacgtcgaga cgctgcgcca
     1801 tctcgtcaac cagggcatgg gcgacaacac gctgcgggcg ctgacttccg accttgccta
     1861 tctggaagcc tggggactgg cggcgaccgg acaatccctg ccctggccgg cacccgaggc
     1921 gctgcttttg aaattcgtcg cgcatcacct ctgggatccg cagcaccgcg agaccgatcc
     1981 ggatcacggc atgccggccg acgtcgacga agaccttcgg agccagggtt ttctcagatc
     2041 cgcaggcccg cacgcgccat cgacggtgcg ccggcggctg gcgaactggt cgacgctgag
     2101 caaatggcgc ggttttgaag gcgccttcgc ctcccctgcc ctcaaatcag ccattcgcct
     2161 cgccgttcgc gccgtccccc ggacgcggcg tcgcaagagc gccaaggccg tcaccggcga
     2221 cgtgctggca aaactgctgg caacctgcac gaccgacagt ctgcgcgatc tccgtgaccg
     2281 tgcaatcctg atggttgcct ttgcctccgg tggtcggcgg cgcagcgaga tcgccggcct
     2341 gcgcagcgag cagctgaccg tcgagccgcc aatcccgggg gacgatggcc cttccctccc
     2401 ctctctcgcc atccatctcg gacgcaccaa gacgaccagc ggcgacgagg acgacgttgt
     2461 ctatctgacc ggtcggccgg tggatgcgct gaatgcctgg atggtggccg ccaagatcga
     2521 gagcggcagc gtgtttcggg cgatcgggcg atgggggacg gtttcgaaac gggcaatcga
     2581 tccgcagtcg gtcaacgcga tcatcaagca gcgggtggaa ctggctggat tagaccgtga
     2641 ggagttttcc gcgcacgggt tgcggtccgg atatctgact gaagcagcta accgcggcat
     2701 ccccctcccc gaggcaatgg aacagtcgct tcatcgatca gtgcagcagg catcaagtta
     2761 ctataacagc gcaacccggc gggaaggtca ggcgtcgcgt cttctcgcct aatcgcacat
     2821 gatcacaggc atggccatcg cgcgccttcg ccttaatacg caagcaatgc attggcggct
     2881 tggtctagcg gggatcgcgc tagccatgtt gggctatgga gtgtcttggc tcgcgctgct
     2941 ttttctgcct gatgtaccca ccgctttgcg ggagtctccc gggttgttgt gggatctcga
     3001 ctgtccctgg tcgctatcgg ttgccgcacc tcacagcgag acgacgttct cgatcctggg
     3061 caacaccggt tgcgcaatga ttgtcttggc ggcttgctgg cttgctatgg attcgctccc
     3121 gcgtttgcga aagatgtttt ggccagtagt cgcggtcggc tcgatgtcgt tgacggtcta
     3181 tgtgctgcac actgtgggaa tagcctatct cctgcgcgtt atgggaatag attcggctgg
     3241 agaagacggg agcctgtcta tgctccttag cttcgtcgtg gctacaagca ctttcgcggt
     3301 gctttggctg cgcgccttcc aacgaggacc gatggaagta ctgatgggta ggatcacaga
     3361 tctcgcgcgt cacatcctct aagcaccacg tcgccccgac tccgatctac ccttgtaggg
     3421 tgctgtgata ccgacctgcc ttcgtgcccg acgcgcgcag tgacggtcaa gtgggccgac
     3481 gatcaatcgc gacgcgacct gcccttgacc gtcacgagta cagcgccaga gtgttctggt
     3541 cggaggcgcc ttaaaccgtg ccgacattag attccgggga ggagctcgcc accagcaggc
     3601 gcgcgcccgg cttagatcga ttggcgaaaa gccgaccgcg aagccgacga gaacggcgag
     3661 cccgacaact gtgtagagcc caaccgcctc cgcggcttgt gctacagtcc ggttttccac
     3721 cggcggatcg catatggcgt aggctgccga tcctgccagg tacaggcatg gcgagcagcc
     3781 atgtgccgat gatacccggg ctgaatagcg cgaatattag tggtgccgaa gagcgccttc
     3841 gcttccgtca gcgcgagctg cgggatcacc gttcgaaacc gcgacgccgg tccagtgaaa
     3901 agggacagca ccacctggag gatccgggaa cttaccgcga ccagcagtgg gaatacctgg
     3961 cggcccggac ccggtaccaa ctccgccgcc tcgcccatag ctgcgagatc gccgccgatg
     4021 ttgatcgtgt ttgcgctgaa cagcagcgcc acgaaggttg ttacggccca ggtcggatag
     4081 atccggcaca tgctgcccgc aattccgcgt cccgtgcgac ccgtcctggc tctaccgcag
     4141 cgagtagagg aacaacatcg tctatagcat gtcaagcaag cgcccacctg cgagcaggta
     4201 gctatcccgc tgggattatc gtcggcttcg ccaatgatcc gccctgaccc aacttctcga
     4261 acagtgtact gcgcggctgt acccgcctat gccccacaac caatgggctc cgagtttcga
     4321 cccaattcga ctgggatgac ggagattcgt tgagacaaag ctgacaaatc tcagtgggat
     4381 gggcctgtct ctgcctcaaa tgctatgcgc gcatagcctc ggtggggagg gctggcgatc
     4441 aggcccacaa ggagcccgcg gcgaagggac gcgtcttcgc ggactctgaa gtctcaattt
     4501 ggctctctcg cgagctgaat gtttgcgctc tgcctcgagg cgagacttgt tccacgcggc
     4561 gcatatgtaa ctccgacata aactaacttc gaaaaaccat taaattcccg taacttaggt
     4621 cggtggtcag ttgaccaccg aagaaaacta gtctcggcgt cgcgaaaact tttgagtcgc
     4681 actatcaatg acgatgagca aagctttacc cgaccttcaa cctgccctgt ccgttcgcct
     4741 tcagcaacgc catcagcatc ggcccaatcg cgaattcgcc cttttcgctt cgccgcgtca
     4801 ggccacgaag atagccaccg gccgaattaa tgtggccgcc tcgctccagg atgcatgcca
     4861 ttacggtcgc ggcgttttcc ggacccatca cattggcggc ctcttcgtag gccgatggac
     4921 tgacgcccag catcgatcga acgatgacgg ctgcggccat cagatcgcgc cagttgcgga
     4981 ttaagccatc tggcccatag gcaaggattt ccgggcaggc ttgcagaacc aggccgagcg
     5041 ggaaggactt cagtccgcca ccatcattgc ggacccccgg ccgtcgactt tgctcttcct
     5101 ccttctgcgc gaagggatgg cctgtctcac cccttccctt cgcggccgtc agagaccctc
     5161 ggtcgttgtc tggctcagct gtcgcgccct gcttcgtttc gaagcttggt tcaagttcag
     5221 aaatagattc gggatttgaa ttctgtatgt gacgctcgat ataattggca ttgccgcgct
     5281 gttttgttga tttaacctgg ttttccagcc ggttaagaat atctgtgcgc aaggccgtca
     5341 attcatccag caacagttca agctgagctg tcgtcggcga tcgcggcagg ccttcgatca
     5401 ggtcccggaa ttcgcggtat aggccttgcc aatccccagg gatctcttct tcaacggccg
     5461 cttcgatcag ttttgcaatg tcacggcggc aaagggtgat gcgttcacgc agacgctgga
     5521 catgcagccg ctcggccatc actgcggccg caagctgttc gatctcctcg gcacgagcaa
     5581 gcagtggcgc gagcgagaag ccaaaagctt catcgatctc ccctgcgctg tccctgcgga
     5641 catagcgctt gccattgggg ctatccttac gcaccagaag acctgcctcg acgagggcgg
     5701 caagatggcg ccgaacggtc tgttcggcca tgccatgcgt tctgagcgaa agctggatat
     5761 tcgacgggaa gacgaccaag ccgttttcct gggccagttc attcttcgga taaaaggtca
     5821 aaagcgagtt gagcacggca agcgcccgat cgccgatgcc aagcagcgcc tttgcctcgc
     5881 atagcgcgcg aaacaatttc cacttatcga ccgactgatc gggtttgatc tcccttgcca
     5941 tgacctgatt tgcaagcatg ccaagcgtca tcgaccgccg cccgaagggc gttacacttc
     6001 ccgtgtgcat tgtcttcacc tctcaaaaag gcaaaagaaa tccactcacc aacacggcgc
     6061 taaaatctct tgacgaggat tcgaggaaat gcgattctat tcttgctaca gagacagaag
     6121 ggcttccgcg acggcgacgt tgagggggct cttttctttt gcggtttatt ctccgtgttt
     6181 cagttggttc tcaaccttgt acgcttgata cagcgctggc acatgctcga gcacgaactt
     6241 cgcaaaatca ggcatcgcct tcctgtcaat cgtgatttcc agtttgacct tgctctgcat
     6301 cacctgtgca attctggtgc catctggggt ggccatgacc tcaggtaatc cgcgcgcaat
     6361 tcgactgggc ttcaaactaa ggatgaccgc cttgaaccgt tctgccgatg gcagcgcttg
     6421 aacttcctcc gacatcgcat atttcgacac gtcggccgga gaaggcgctt tctcgatcag
     6481 ttccgcaagt tgttgccaac tcggccgccc aacaccagga gccgcaccaa tagcatcgat
     6541 cagttcagag gggagggcgt ccacgagcag aagcatcttc gacaaattgc tcttgtcgat
     6601 cgacatcgcg gcgatgacaa tctctcttga aaactgccta ttcaggtgat gtgcgaagcg
     6661 cgccttttcg atgaaggtaa gatcttcgcg ctgattgttt tcctgaccct gcgctacgac
     6721 cagttgctcg tctgtcagtt cgcgaacaac cgccttgacc gggattccga gctctgaaac
     6781 tgcgcgcagc cggcggtggc cgaaagcaat ctgatatcgg gcgggctggt ccggatgagg
     6841 tcgtacaagg atagggacct gctgtccttg ctcgcggatc gaagcaacga gcccttcgat
     6901 gtccccaggc atacgatcct gcacgaaaga cggctcaatt gacgaggcat ccaactctat
     6961 cactacctgc ccttcagcaa gacgccgctc gatctcttcg gcacggctaa gacgatcgtt
     7021 ttgctctcgc agcgcgtttc cgatgttcgc tgtgagcttc gttgcgggat cgcgctcctt
     7081 ccttgccacc ccgagaagcg gcatggatcg attctttgcg gccctgtagt ctgcggacga
     7141 cgtttcgggc gcgtctgttg agacgccaag gatgtgcttc cggctcatgt gggtctaccc
     7201 catgcttttt tgatcagtgt ttcgatttcg tcgttgacgg cattcatcgc ctccaacgct
     7261 cggtcatagg tcgaacgggt gaactggcca cgctcgattt caaatagggt ctggtttgtc
     7321 agaccagcat ccgaaaccgc ggtggtttta agcattggaa aatttagaac attttcgccg
     7381 aaaatagacc gcagataacc gaccatctga ttttgcggtc catcgctcgg ttcgaaacgg
     7441 gttatcaagt agcgcatcca attaaacttg aactctgcgc cagcgttctc gatttcgcgc
     7501 agaaggtttg atgtcattgc cagaaactgg ttcatcgaca tcacgtccag catctgagga
     7561 tggacagtga ctaggatcga tgttgcggca gtcagagccg aaagcgtgag gtaaccaagt
     7621 tggggagggc agtcgatcac cacgacgtcg tagttgtcag cgatatcttc gatcacctga
     7681 ctgatacgac cgtaaaagag cgtgtcgcct tccttgcgct tcatcagcgc acgcggcgtg
     7741 tcgtgctcga attccatcag ctcaagattg cccgggatca ggtggagatc agggatatac
     7801 gttgctcgga caacctgctc gataggaacc tgctcttcat cgtatctgat ggcgccgtaa
     7861 agcgtctggt tcggaccaac gtcggtttcc ggttggctgc cgaagagcgc ggaaagactc
     7921 gcctggggat ccagatctat ggccaggacg cgatatccgc gcatagcgag atactgtgcc
     7981 aaatgcgcgg cggtggtggt cttgccagat ccacctttga aattcatgac agatataact
     8041 tggagccgct ctccgtttcg acgatgcggc aggtagcgac ggttcccgcg tccgacccgg
     8101 tccatatgct ttcgaatcgc atggatatct tcaattgaga acattcgcct gccaccgggg
     8161 ctcatgctaa gattcaactc tggcatctcc gacgcggtct ggcgcaaata ggactcgcca
     8221 acgcccagca gcttagacgc ctcggacggc ccgaatgttc gaatgccctt ctcggaatgc
     8281 ggtgggaaga ccttaagatg atgtgcttga agttggctgg agagagcgtt ggcatgacgc
     8341 tccatcaagg ctgtcaatcc ttcaattaca ggtacttctt tttcggcggc cttcgccata
     8401 tcaaaaccca tttgttgcca ctggcggtat ttttcgcgaa actggtaaag ttccgccacc
     8461 ggcaataagc gccgattcga atttttgagc aagggctttt aggttaacga aaggttaacg
     8521 cgaaacccag gtacacgcca aacacgagaa cttttacgtt ctttatctat ggtttagcaa
     8581 ggccgatatc ccggtaagtt catgatctgt tttactgtga atcctgcaac cttccctgtc
     8641 gtttcggcgc atagggagag catgacccac cgttcgcacg gcggagactc catccgcctc
     8701 gaagagctta gccggttcgt acaacatttg agcgtatgcg ctgcgtacgg ggctcatttg
     8761 ctccgctccc tggattgggg gttcgcctca cctgcagtcg tcgatcgcga agtctgcgac
     8821 acaaagatcg ggggacggca cccccgcgcc tgcatgcgtt cccagacttc tttggcatgg
     8881 cgctgaatgc gcttcgaagt ggtttccatg tgattcttca caacgttaca gggtggctct
     8941 gatttccatc gtgacggttt ttataccaca tgatggatat attccgccgc tgtgtaaacg
     9001 tcttgcgtcg acgagtcaaa tacaaaggac atttcccaag ctatgaaaca ccaaatccat
     9061 cggcgcaggc cggtcgtggc cctcaattgg ccttgccaag ttgtcgcttg ggacggtaac
     9121 gcatttccag tccaatcccg cgaacagcac tgagaactgt gctgccgtca gatgcatcgc
     9181 gccgtcctgc actcgaggcc aatggaactc gccatcctca agccgcttgg caacccggca
     9241 cacgccgctg ccatcccaga agatcagcat gcattgcgga agtcgactgg attagtggcg
     9301 atcatcacct ttaccacacc cgacgtacca acattgagcc ttcaacgccc ggatagcgcg
     9361 agacaatcgt tgcgtcatcg gtgccagagg cgatgcgcaa cgtggctcca tcaatttgca
     9421 agttgatagc cgcctccgga gaagcctttc ttggcgaaag tgcctgccgc gccttctttg
     9481 gctccggctt ctcgggcggc acgacgaccg ctggcgcaaa catcggacct tcccagcaca
     9541 gaatctccgc tggctttcac ggcgagccgc cgccatctga acaattgctt aggcgtcaac
     9601 ccatgccggc gtgccacaga gcacgccgtc acgccaaggc tcgcaactct ccgcaataat
     9661 ccgcgccttc tgatcgtcgc cccattctcg acgacagcaa ttgccagtga acacctcaat
     9721 ccggcgtacc agctcacctt ggatttaagc gtaagctcgg gaatcgacac atgtttaaac
     9781 cctctcacga ggctgaacat caatgatcaa gcgatgagcc agacgatggg ggatcaggat
     9841 agtgtttacc ggcattctcc aggggcgata tggctgcatt cgacaaattt ggaatatgtc
     9901 gaagcgcgac cctctcccag cgagtttgta ggattctaca acttcccctc aattccagtc
     9961 tgtgctgatt tccagccgcc caacaatgac ggagaaaatc atgcagattc tcgcgctctc
    10021 gacaccacgg acgaccctag aggcgcatct cctacacatc cactaccaac ttcgtgctcg
    10081 gatcttttcc gatcgcctgg gttgggaagt ggacgtaatg gccgggtgcg agtccgatcg
    10141 tttcgacgcg cttcggccga cctatgtgct cgcagtcgca gagacgggcg aattggcggg
    10201 gtgcgcgagg cttcttcctg cgctcggacc gacaatggtg gccgacgttt tcccgtcgct
    10261 gctccccgac ggccaactca aggggcatgc cgcgatggtt gagagttctc gcttctgcgt
    10321 cgacacgaga attacggaag ggaggggcga cggttcggtt catgaagcga cgctcaccat
    10381 gttcgctggc atcatcgaat ggtgcatcgc tagtggctat acggagattg tcacggtgac
    10441 cgatcttagg tttgagcgaa tcctcgctcg cgtcggatgg ccgctgcagc gtttgggcga
    10501 acccaagaag attggcgtga cgatggccgt cgcggggaca ctggccgccg acgcagacac
    10561 cttcctcagg ctttgcccct ccaactacca ttctgaattc gccccctgtc agccaggcgg
    10621 cctaaggaga aatccgtgaa ccagcttcgc tctcaccctc ggctcgtccg caaacttcag
    10681 gaggcgctcg gcgatcagct ttgtgttgcc ctggacgacg cgaacgtagt cgaaatcatg
    10741 ctcaatccgg acggcagatt attcattgaa cggctaggtc acggtgttgc gcctgcgggc
    10801 gagatgtcat ccgctgcagc ggagatggtg atcggtacag tggcgcacgc gcttcagtca
    10861 gaggtcggca cggaacagcc aatcatctcc ggggagctgc caatcggtgg ccaccgcttc
    10921 gagggactgt tgccccccgt tgtcaccaag cctgccttca taatccgtcg cagggcgtcg
    10981 cgcctcattc cgctcgaaga ctatgttcgc accggcgtga tgacagaata ccacgccgca
    11041 gcgatacgca gtgccatttc cacgaggctg aacatcatca tttctggggg gacgggctcg
    11101 ggcaagacga cgcttgcgaa tgcagtgatt gacgagatcg tcaaatctgc gccggaggat
    11161 cgtctcgtca ttcttgagga taccgcggaa atccaatgcg cggccgagaa cgccgttctc
    11221 ctccacacca gcgattcgat cgacatggcc cggctcttga agagcactat gcgcttgcgc
    11281 cccgaccgga tagtcgttgg cgaagttcgc gacggcgcgg ccctgacgtt gctcaaggcc
    11341 tggaacaccg gccacccggg cggcgtggcg accattcact cgaacaccgc catgtcggcg
    11401 ctgcgtcggc ttgaacagct gaccgccgag gcaagccagc agccgatgca cgaggtgatc
    11461 ggagaggccg tcgacctggt catctcgatc gagcggacgc cgcgtggccg gcttgttcgc
    11521 gacatcatcc aagtcgagcg gttcatcaac ggacggtacg agatcgaatc cgatcagttc
    11581 accgaagaac aggaggcgcg ccatgtcgcg tgagcatacc ctcatcgccg ccgcgctctg
    11641 ggccgcgcca atcgtatttg cctcggtcgc gccggcgctt gccagctccg gcggcagcct
    11701 cccatgggaa ggaccgctgc aacagatcca ggagtcgatc acaggcccgg tcgcgggtgc
    11761 aatcgcgctg gcagccgtgg ccatagccgg cggcatgctc atcttcggcg gcgagctcaa
    11821 cgatttcgcg cggcgacttg tgtatgtcgt tctcgtcgcc ggcatcctgc tcggcgctac
    11881 caacatcgtc ggcctgttcg gtgcgaccgg cgcttcgatc gggctaaccg acgcgcagat
    11941 cacgtcaatt ggtgcgaacg gaggagggga gggagatcat ggctgaatcc ctttccggcc
    12001 tgcggcgcaa tcgcatccac cgcgcgctgt cgcgcccgaa cctgctgatg ggcgccgacc
    12061 gggagctggt tctgatcacc ggccttgcgg ccgtgatcct gatcttcgtc gtgcttacgg
    12121 cctattcggc actcttcggc gtcgctgtct gggtcgtgat cgtcgggctg ctcaggatga
    12181 tggcgaagtc cgacccactg atgcggcaag tctatatcag gcacatttcc tacaagccct
    12241 attacaaggc gaccacttcg ccgtggcgac ggtactgaga aggcggccat ggtagctctc
    12301 aaacgcttcc gggtgaccgg cccgtccttc gccgatctcg ttccttatgc cggcctggtc
    12361 gacaatggtg tcctcctcct gaaggacgga agcctgatgg caggctggta cttcgctggc
    12421 ccggactccg aaagcgcgac cgacctcgag cgcaacgagc tgtcgaggca gatcaatgcc
    12481 gttctgtctc ggctcggaag cggctggatg atccaggtcg aggccatccg tattccgaca
    12541 gtcgactatc catcagaaga tcgatgccat ttccccgacc cggtaaccct cgcaatcgat
    12601 gccgagcgtc gggcgcattt cgcgcgcgag caggggcatt tcgagagcaa gcatgcgcta
    12661 gtcctgacct accggcccct cgagtccaag aagactgctc tcagcaaata catctattcg
    12721 gacgaggaaa gtcggaagaa gtcctacgcg gacacggtgc tattcgtgtt caagaacgcg
    12781 gtgcgagagc ttgagcagta ttttgccaac actctttcga tccggcgaat ggaaacccgc
    12841 gaaacggtcg aaaggggagg ggagcgaatc gcccgatacg acgagctgct gcagttcgcg
    12901 cggttctgca ccaccgggga aagccatcca atccggcttc ccgatgttcc catgtatctc
    12961 gactggatcg ccactgcgga gcttgagcac ggactgacgc caaaggtcga aaaccgtttc
    13021 ctcggcgtcg ttgcgatcga cggtctgccg gccgaaagct ggcccggtat tctgaacagc
    13081 ctagacctga tgccgctgac ctatcggtgg tcatcgcgtt tcattttcct tgatgccgag
    13141 gaggcccggc aaaagctcga acgcacccgc aagaagtggc agcaaaaggt ccggccgttc
    13201 ttcgaccaga tattccagac acaaagccgg tcagtggatc aggacgccat gagcatggtg
    13261 gtcgagactg aggatgcgat cgcgcaggcc tcgtcgcagc tggttgctta tggctattac
    13321 acgccagtcg tcgtgctgtt cgacagcgat cgcgaggcac tccaggagaa agcggaagcg
    13381 gtccgccggc tgatccaggc ggaaggtttc ggggcgcgga ttgaaacgct caacgccact
    13441 gacgcctatc tcggcagcct gccgggcaac tggtattgca acatccgtga gccgctgatc
    13501 aacaccagca atctcgccga cttgatcccg ctgaactcgg tctggtccgg aagcccgatc
    13561 tccccatgcc cgttttaccc accgaatgcc ccgcccttga tgcaggttgc gagcggatcg
    13621 acggcgttcc gtctgaacct gcatgtcgat gatgtcggcc acacactgat cttcggtccg
    13681 accggttcgg gtaagtcgac gcttctggct ctgatcgctg cgcagtttcg ccggtacgaa
    13741 aatgcgcaga tcttcgcctt tgacaaaggc agttcacttc tgcccctgac gctcgcagcc
    13801 ggcggcgatc actatgagat cggcggcgac aatgccgaag aggggagggc gttggccttt
    13861 tgcccattgt ccgaacttaa aagtgatgcc gaccgggctt gggcgacgga atggatcgag
    13921 atgctggtcg gcctgcaggg cgtcaccatc accccagatc atcgcaacgc catctctcgg
    13981 cagatcggcc tgatggcgag tgcatccggt cgctcgctct cggatttcgt cagtggcgtg
    14041 cagcttcgcg agatcaagga cgcgctgcat cactataccg tcgatggccc aatgggccag
    14101 ctcctcgatg cggaagaaga cggcctcaca ctcggcgcct tccagacctt cgagatcgag
    14161 cagctgatga atatgggcga gcgcaatctc gtgccagtgc tgacctacct gttccgccgg
    14221 atcgaaaaac gtttggatgg gtcgccgagc ctgatcgtgc tcgacgaggc gtggctgatg
    14281 ctcggccacc ctgtattccg cgacaagatt cgcgagtggc tcaaggtgct ccgcaaggcg
    14341 aattgcgccg tcgttctcgc gacccaatca atctccgatg cagaacgatc cgggattatc
    14401 gacgtgctga aggaatcctg ccctaccaag atttgccttc cgaacggcgc cgcccgcgag
    14461 tctgggacgc gtgaattcta cgagcgcatc ggcttcaacg agcggcagat cgagatcgtc
    14521 gcgaccgcca tgccgaagcg cgaatactac gtcgccacgt cagaaggccg gcggctcttc
    14581 aacatgtcgc ttgggccagt cgcgctgagc tttgtcggcg cgtcgggcaa agaggacctc
    14641 aaacgcatcc gcgcactgaa atccgaacat ggccgcgact ggccgatcca ctggcttgaa
    14701 acgagaggag ttcacgatgc cgcatcgcta ctcaagtacg tatagatggt ttgcatgcct
    14761 agcggccgcc acgctcacga tcgtaaccgc aggtccggtg caagccggga cagccaccgg
    14821 cgctgcgacg gaatggacgc agctcgccaa caatgcgcag ctcgtagatc tcatgaaaag
    14881 ctccggcatc caggtcgaca atcagctgac tcagatcagc cagcttgcag agcagatcca
    14941 gaaccagctg aagatctacg agaacatggt gcaaaacact gcgcagcttc ctgaccatgt
    15001 ctggggtcag gtcgaaagcg atctcaacca gctgcgcagc atcgtcgacc agggacaggg
    15061 catcgccttt tcgatgggga atgcggatga cgttcttcag cagcggtttc agagttatgc
    15121 cgatctcaag acgaatttgc ctgacaacgc gacgttctcc tcgacctacc agtcctggtc
    15181 gaacaccaac cgcgacacga tcgccagctc gctgaaagcg gcgagcctca cggccgacca
    15241 gttcgatagc gaggaagaca cgatgtcctc gctgcggtcg atgtccgaaa cagctgacgg
    15301 gcagatgaag gctctgcaag tcgggcacga gatcgcagcg caacaggtcg cacaaatgca
    15361 gaagcttcgc ggtctcgtct cccaacagat gacaatgatg ggaacctggc ttcagacgga
    15421 acagaccgac aaagacctgg cgcaggcgcg gcgggaaaag ttcttcaacg ccgaggtcaa
    15481 aagcgttccg gaaggccaga aaatggaacc gcgatggtga gccgctccgt tctgattgcc
    15541 ttgctgttgg ctgtcgccgc tgcatcgtct gcagcgacag tgctgatcgt caattcccga
    15601 aacaccggaa tacctgcgct caccgaggag cagcgcgccg tccgggaaaa attcttcggc
    15661 tccgacaagg atctgccgcc gatcaaggaa ggccaggaga tgcgcccgag atggtgaatg
    15721 taactgttgc gcgttctttc ctgattacag gtctcttttt cctcgcctat gctgttcccg
    15781 cattcgcgca ggaggggcag gtcctcacgg aactggaaaa tcaggtctcc tccgctgcga
    15841 aggggtggga gaccaccatc atggaggcgg cgaaatccct attctggatt ctcgcaacga
    15901 tcgagatcgg aattgcggcc gtctggctgg cgatccaatc ggcctcgctg gacagctggt
    15961 tcgccgaact ggtgcggcgg atcatgttca tcgggttctt cgcattcgtt ctgagccaag
    16021 gtccgacctt tgcgcgagcg gtggttgata gtcttttcca gattggcgcc ggtgctggtt
    16081 cagcgtcgcc cgcggaagtg ttcgatgcag gcatccgggt cgcctcgcag atgtcggagc
    16141 aggcacagtt cggggtgttc gaagacaatg cgcttgcgat cgcggcggtg ttggcgatgg
    16201 gcatcgtcgt tatctgcctc tcactggtcg cagcgatttt cgtgtcggtc atggttgaga
    16261 tgtatgtcgg cctgctcgcc ggcatgatca tgctcggact gggcggctct tccttcacga
    16321 aggactttgc cattcgctac ctcgtctatg ccttcggcgt cggcatgaag ctgatggcct
    16381 tagtgatgat cgccaagatc ggatcgaacg tcctgcttgg ccttgcccaa gcgcccaccg
    16441 cgtcgtccga tcagttcgtc acgaccttgg ccatcgccgg tatctccgtc gtcgtcttca
    16501 tcatcgcgat gtatgtcccg aacatcatcc agggcgttgt ccagggcgca tcggtgtccg
    16561 gaggaatgga agcgatccgc cacggcgggc aagtggcgtc ttttgcggcc ggtgctggct
    16621 tcctcgccgc cggcgctgcc ggcgcgggat ttgctgcggc tcaagccgca cgagctggcg
    16681 gttcatccgt tgcaggtgct gctcttcgcg gcgtgggcgc gagcttctct tccggcgcgc
    16741 aagcagccgg agctgccgcg aaggaaaagg caattggctc tcccggcgct tatgccgggt
    16801 ccattctcgg actggccaat gcgaagctcg atgaacagcg cgccggtcat agcggaccga
    16861 aacctcctcc cgaacgcaac gacaaaccat aatcgagaca agggaagaat aaatggcagc
    16921 gaaccgcgcc ccggaaaacc cgtatcttgc cgcccgccag gaatggagcg aacgctatgg
    16981 ctcctatgtg aaggccgcag ccgcatggcg cattgttggt gtcctcagcc tggtcatggc
    17041 cgtcatcggc ttcagctacg cgatgtatct gagcacgcaa gtgaggctcg tgccttacat
    17101 cgtcgaggtc gacaagctcg gaaccgcagt cacggcaggc tttccggagc agatcgagta
    17161 tgccgatgtc cgcgtggtgc gcgccacact cggtaacttc gtcacgagct ttcgctcgat
    17221 cacgccggat gcggtggtgc agaagcaata tatcgaccgc acctatgcgc ttcttcgcac
    17281 ctccgacccg tcgacggaga aggtcaacgc ctggttccga ggcaattctc cgttcgagaa
    17341 ggcgaagact tcgacggttg ccatcgaggt caacaacatc gtggcgctct cgaaccagac
    17401 ctatcagatc gactggacgg aatacgagcg ggaccgcaag ggcaaggaaa ccggcacgcg
    17461 gcggttccgc gggatcgcaa cggtgacgct caccgcgcca caggatgagg cgacgatccg
    17521 cctcaatccg atcggcctct acgtccgaga ttttgactgg acggcacagc tttaagggca
    17581 gggattcttt caatgaaaaa aacgggattg atagcagccg ccggctgcat agccggactc
    17641 ttgcttgcgg cgggcgcgca ggcgcaaagc atgacgagca acgaggtgaa gggaacaaat
    17701 ctttccagga agtggcgtgg cacgccggga ctggtcacga caggtccgga cggaaaggtg
    17761 atcttcctgt tcggcgaaac gcaaccttcc gtcgtctgct cgccgcttca ggtctgcgac
    17821 atcgaacttc agggtggcga gatcgttcgc gacgtcctcg tcggcgacac cgtgcgctgg
    17881 aaggtggagc ctgccacttc gggggccgca ggcggacagg ccatccatct gatcgtcaaa
    17941 ccgtcggagc caggccttct cacctcgatg gtcgtgacca catcacggcg cacctatcat
    18001 atccagctca agtcccatcc cagccagtac atggcccgca ttggtttcga atatccggaa
    18061 gacgtgtcga ccaagctcgc cgacatcaac gcccgtctcg aaaccggctg cattcctggc
    18121 accgcgccgg acaagctgaa cttttcctat tcaatcagcg gcggtgcttc gtggaaaccg
    18181 aagcgggtct attcggacgg ggtgaagacc tacatccagt tcccgaaatc gatctccgga
    18241 caagacgcgc cggtgctctt cgtcgtctcc ggcggtcaaa atcgtatcgt caattaccgg
    18301 atgaagaacg acatgatgac tatcgactat gcgatcgaca gggcaatcct cgtttccgga
    18361 gtcggttggc ggcagcagaa gattaccctt cggcggggag gctgaaccat gcggaagcta
    18421 ttcgcattct tcatcgcggt cgcgctgctc tccagctgcc aaacggccga cgacgcgctc
    18481 accaccagct cgacccctgt ggccgtcacc ggaccggctg caagcgccat cgccggcgac
    18541 atggcaagtc gtctggctga acagatcggc ccggcagggg ctacgaccac gatcaaaatg
    18601 gagacggacc cgtcagagtt cgcgaccgcc cttaaggcgg ccctgaaagg gtggggctac
    18661 acggtcgtca cggacggcaa agtcgccaaa gacgtcaagc cggtcgagct tgcttatgca
    18721 atcgagggct tcgacggaga ggtgctagtg caggtcacga cgccttccat cgctcttggc
    18781 cgcgcttaca cgccgacggc ggccggcgcg acgccggcca gtcccctttc gatcatgcag
    18841 cgcaactgac ggagtttaat atggtccagt cgctccagct tggcacctcc agccaagccg
    18901 acgatcacca gggcatgcgg cggctcaacc gcctgccgat catcgtcgcc attattttca
    18961 tcgtgctgtt cgtcggcgtc gttgtgattg gtctgtcgct gcgcgggctt tccttcaatc
    19021 gtggcgacat cgagagcgct tccaacagcc ctgcgaccag tttcggcgac cagcttaagc
    19081 ggggtgtcac ggacggcatt atcggtgacc cagaaaagca ggaggtgttt cagccaacac
    19141 ccctcgtcat ggagaaagag gaaaagcacg ggccggtcgt cgagcgccga ccagaggagc
    19201 gacaagaccg tcgacaaagg ctcgaatccg aagaggagtg gaaggctcgg ctgaagcgag
    19261 agcaggatga gcaatacatc cgggaagcgc agcgccagcg aatggcgcgt cttcaggctc
    19321 gttccacggc gctcgattcg ccgctgaagg tcgacatgtc cgatgtcgag aaatccgcaa
    19381 actccaccaa cgacaccagc cgtcagccga caaatgtcgc agcaaacagt gcatcagacc
    19441 tttttgccgg cgccatgaaa tccggcctga tgggcctgaa cgtcgatcag aacgggcaga
    19501 cctcgaaaga agatttcttc aatcaggaca tcaaggatct cggctacctg ccgaaccagg
    19561 tcgtgcggca aatctcgccc tacgaattga agcgcggctc agtcattccc gccaccttga
    19621 tcacaggcct taattccgat ctaccagggc gcattaccgc tcaggtcagc cagaatgtct
    19681 tcgacagcac gaccggctat tggcttctga tcccacaggg tgcaaagctc tttggccgct
    19741 acgactccaa agtgttattc ggccaggaac gtgttctcgt cgtctggacg gacctcattt
    19801 tcccgaatgg gtctaccctt caaatcggcg gcatggctgg cacggacgcc gaaggatatg
    19861 ggggcttcaa ggatgaggtc gaccgccatc tctggaggac tttcggttcg gcggcgctgg
    19921 tggcaatcat cgggaccggg atcgatatgt cgatgcccga aagttcgacg ctggcgacgc
    19981 aggacaccgc ctcggatgca gcacagcgaa aatttgccga atcattcggt cgagtagcgg
    20041 aagagacgat ctcgaagaat ctgaacgttc cgccgacggt tcggatccgg ccaggctaca
    20101 agttcaacgt catggtcgat caggatattg tttttccatc tgtctatggc gaccgctaac
    20161 cagttaagga cctcatattt tcgattgtag ctgaacgtcg caagtgcatt ccgcgctgcg
    20221 gcgcaggtgt gactaaaaat ctctacaaaa atcatcttct ttagatcaga aagagttgaa
    20281 tacttggcag aggtcaatag acctaagcga tgcaacgaac gtggatctgc tacgcgtaga
    20341 agcaactgcc gtatcgtcca atatcccatg tggttccggc tggccgaacg gtggcaaccg
    20401 gtacctcgcg gcgtcagaac ggcgcgacga gtgggaggct tgcactgtcg aaaaaggaga
    20461 aatgttagtg gacggtgacc ttcgctctct catcgatatg acagaagctg cgcatgatga
    20521 gcgtatgatc aaaagtgctt tgaagacatt tgcgcacgca tgcggcctcg accgttttgc
    20581 ttatctgcaa actgagggtt tggaaatccg cacattcaac acctatccgg aggaatggc
//
