LOCUS       NZ_SIJR01000001        10387 bp    DNA     linear   CON 04-JUN-2024
DEFINITION  Rhizobium leguminosarum strain SM4 chrom_SM4, whole genome shotgun
            sequence.
ACCESSION   NZ_SIJR01000001
VERSION     NZ_SIJR01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhizobium leguminosarum
  ORGANISM  Rhizobium leguminosarum
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
REFERENCE   1  (bases 1 to 5107752)
  AUTHORS   Cavassim,M.I.A., Moeskjaer,S., Moslemi,C., Fields,B., Bachmann,A.,
            Vilhjalmsson,B., Schierup,M.H., Young,J.P.W. and Andersen,S.U.
  TITLE     The genomic architecture of introgression among sibling species of
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5107752)
  AUTHORS   Cavassim,M.I.A. and Young,J.P.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2019) Biology, University of York, Heslington,
            York YO10 5DD, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.7; Jigome v. 1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCF_000023185.1
            Genome Coverage                   :: 39.9959x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004307035.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 04:40:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,389
            CDSs (total)                      :: 7,324
            Genes (coding)                    :: 7,141
            CDSs (with protein)               :: 7,141
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 89 of 183
            Pseudo Genes (incomplete)         :: 135 of 183
            Pseudo Genes (internal stop)      :: 23 of 183
            Pseudo Genes (multiple problems)  :: 56 of 183
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2886455
            Orig. end                         :: 2896842
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SIJR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10387
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="6"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10387
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10387
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5387](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5387)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            226..399
                     /locus_tag="ELG62_RS37690"
     CDS             226..399
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540992.1"
                     /locus_tag="ELG62_RS37690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_012758079.1"
                     /transl_table=11
                     /translation="MSKTNARQLQKRAEQQVKNTSAGGHLGGTYFGQQPDGKTASSTNN
                     DSAKARPNDGSK"
     gene            578..1510
                     /locus_tag="ELG62_RS14665"
                     /old_locus_tag="ELG62_14670"
     CDS             578..1510
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988809.1"
                     /locus_tag="ELG62_RS14665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14670"
                     /product="hypothetical protein"
                     /protein_id="WP_028740985.1"
                     /transl_table=11
                     /translation="MASYSIDDAIRELAPALGKAPAGAVSGDWTATTMQAGHSSRTGGY
                     LDAEGKHVPEDSRHPLDIIADVVEKLDASALQRFNKVVIRWKKPKFPFMQAKITLETSY
                     DQTIVPRGPDDPIYETAAATRRVFWQSRGTLHEDFAVERGTANIHAQTKWFGPHRRILA
                     IHAPERLILATDGLSTPWAGISEPENGVECELFMEFDAATLDAAGIENWANLLINIGDL
                     VADGYRVARDVEKHGAILFCRLTEDYHPMTRIMLSRNPGRIDRLPFGSVPLIRATPIAE
                     TEIEGQDLSYDWGAAAARNALAKRGLGID"
     gene            complement(1557..2000)
                     /locus_tag="ELG62_RS14670"
                     /old_locus_tag="ELG62_14675"
     CDS             complement(1557..2000)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (4-141). E-value:
                     6.1e-08. Score: 25.1. Matches aSDomain:
                     nrpspksdomains_ELG62_RS14670_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242667.1"
                     /locus_tag="ELG62_RS14670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14675"
                     /product="SRPBCC family protein"
                     /protein_id="WP_025395108.1"
                     /transl_table=11
                     /translation="MPSTIRLHRVLATSPEKVYRAFLEADALAKWLPPNGFLCTVHHSE
                     PVVGGTFRMSFRNFTTGNSHAFGGEYLELVPGERLRYTDKFEDPNLPGEMEVTVTLKKV
                     SVGTEVDIVQAGVPDVIPPEACYLGWQESLRNLARVVEPDIQE"
     aSDomain        complement(1578..1988)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ELG62_RS14670_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="6.10E-08"
                     /label="ELG62_RS14670_Polyketide_cyc2.1"
                     /locus_tag="ELG62_RS14670"
                     /protein_end="141"
                     /protein_start="4"
                     /score="25.1"
                     /tool="antismash"
                     /translation="IRLHRVLATSPEKVYRAFLEADALAKWLPPNGFLCTVHHSEPVVG
                     GTFRMSFRNFTTGNSHAFGGEYLELVPGERLRYTDKFEDPNLPGEMEVTVTLKKVSVGT
                     EVDIVQAGVPDVIPPEACYLGWQESLRNLARVV"
     misc_feature    2089..2102
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.21"
                     /tool="antismash"
     gene            complement(2118..>2216)
                     /locus_tag="ELG62_RS38160"
                     /old_locus_tag="ELG62_14680"
                     /pseudo=""
     CDS             complement(2118..>2216)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242668.1"
                     /locus_tag="ELG62_RS38160"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14680"
                     /product="aldehyde-activating protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="REEIEPGGEMFIRRRLTCARPLDLPLFDGMPG"
     misc_feature    complement(2244..2253)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 16.86"
                     /tool="antismash"
     misc_feature    complement(2418..2432)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            complement(2506..2712)
                     /locus_tag="ELG62_RS14680"
                     /old_locus_tag="ELG62_14685"
     CDS             complement(2506..2712)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485702.1"
                     /locus_tag="ELG62_RS14680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14685"
                     /product="hypothetical protein"
                     /protein_id="WP_130787295.1"
                     /transl_table=11
                     /translation="MPDDEKILIESITSPHHTERVNKAKYMAMRHALLAALPEGAPGLT
                     VAEANIAILPHLSEIEFPGCARA"
     gene            complement(2705..3244)
                     /locus_tag="ELG62_RS14685"
                     /old_locus_tag="ELG62_14690"
     CDS             complement(2705..3244)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017994570.1"
                     /locus_tag="ELG62_RS14685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14690"
                     /product="hypothetical protein"
                     /protein_id="WP_011652592.1"
                     /transl_table=11
                     /translation="MNNKTTHLTCACGTFHLEVRGAPIISAECHCKSCSDAAGRLESLP
                     MPEAIRTIHGGTRYVLYRKDRISLLAGTENLREFRLGPNASTRRVLASCCNTPIFLEFK
                     GGHWLSLYGNLWKQDELPPLQLRTMTQNVPDRASVPDDVPSGALATAGFYGRLLTAWIA
                     MGFKVPKLTVKGAIDA"
     misc_feature    complement(3308..3321)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.82"
                     /tool="antismash"
     misc_feature    complement(3361..3375)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 20.1"
                     /tool="antismash"
     gene            3368..4036
                     /locus_tag="ELG62_RS14690"
                     /old_locus_tag="ELG62_14695"
     CDS             3368..4036
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988813.1"
                     /locus_tag="ELG62_RS14690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14695"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_130787296.1"
                     /transl_table=11
                     /translation="MLIPGMEADLAERRAGTRSERKQAAIVGAAAEVFLSTGYAGASMD
                     EIASRSGVSKQTVYKHFSSKEALFVAVLTQMMGEADTAVHTGLPQVENRAQLEAYLLDY
                     AVRQLTIVLTPGLMQLRRLVIAEAQRFPELAKMLYARGPARALEVMGSAFEQLAGKKLL
                     QFSDAMVAASQFNWLVMADPVNRVMMLGDAAIPTKQQIYRHAEAAVATFLAAFLHPEKR
                     "
     gene            4264..4761
                     /locus_tag="ELG62_RS14695"
                     /old_locus_tag="ELG62_14700"
     CDS             4264..4761
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017994572.1"
                     /locus_tag="ELG62_RS14695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14700"
                     /product="DUF4168 domain-containing protein"
                     /protein_id="WP_130853099.1"
                     /transl_table=11
                     /translation="MNMITRYTSVAALSATVFSLLAFSPASAVDMAQAQQQPAQAQPPA
                     QGQTPMQGQTGGNGTAAPVSDQKIEAFAVAYLQVDKVRQEYSAKIGATKDEAAKQQLQE
                     EAKKQMVDTVQASPDISVEEYSSILTAAQSDPALAKKVLEKIGTPPLGQQQPQQGQQQP
                     QQ"
     gene            complement(5001..5387)
                     /locus_tag="ELG62_RS14700"
                     /old_locus_tag="ELG62_14705"
     CDS             complement(5001..5387)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007632871.1"
                     /locus_tag="ELG62_RS14700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14705"
                     /product="ectoine synthase"
                     /protein_id="WP_011652595.1"
                     /sec_met_domain="ectoine_synt (E-value: 1e-32, bitscore:
                     111.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVRNLKEVELTERFVDWGNGTSHRLLTKDDGMGFTVCHTVVRAN
                     SEALLQYRNHLEACYCIKGEGEVEDMDGNVFSIRAGDIYVLDKHDRHYLRGGKDQDLVL
                     VSIFNPPLTGTERHDLNNKSGSQY"
     misc_feature    complement(5509..5524)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 20.61"
                     /tool="antismash"
     gene            5517..6395
                     /locus_tag="ELG62_RS14705"
                     /old_locus_tag="ELG62_14710"
     CDS             5517..6395
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050612.1"
                     /locus_tag="ELG62_RS14705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14710"
                     /product="LysR substrate-binding domain-containing protein"
                     /protein_id="WP_028740982.1"
                     /transl_table=11
                     /translation="MQLPPLNALRAFEATARLMSLSKAGDELHVTHAAISHQIKHLETW
                     LGRKLLQKSGRGITLTAAGGEYYRAVSGSLAAIAHATGNMRRDHDMRAITVGCIPSIAS
                     RWLVPALPSFQESDPDLDVRIVYARAEERFDDESLDVLITMGEDLSGGRESRLLFSRRN
                     QPVASRHYLAKRNWTIGDVSLAGADLLHDESVDGWKEWFRKAGQKAPEPLRGPMFQDFN
                     MLATAVIAGHGVALCPVEVFRREIERGDLLVLSDIATAENQGYYVISNSWAKKPVRRFI
                     DWFMTECGSGA"
     misc_feature    6549..6563
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: medium, score: 21.73"
                     /tool="antismash"
     misc_feature    6605..6619
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.88"
                     /tool="antismash"
     gene            6619..8274
                     /locus_tag="ELG62_RS14710"
                     /old_locus_tag="ELG62_14715"
     CDS             6619..8274
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025: diguanylate cyclase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017961068.1"
                     /locus_tag="ELG62_RS14710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14715"
                     /product="EAL domain-containing protein"
                     /protein_id="WP_011652597.1"
                     /transl_table=11
                     /translation="MRKIHAAPSALGQTEAKASSSIDRLLFFDRDFNPVENLLGNELPA
                     SVKPAASFWEHFPELDKSAITLALRSLGDSAQPLQISGDLGAPGSHGLTIYRIGDLFAV
                     VRSEESIDDERATLLHLATHDPLTGLPNRRQFSEDLAVLLRETAGSDETLSLMQLDLDD
                     FKPVNDTLGHPAGDKLLQLAASRIQECLTRDDRAYRLAGDEFTVISRGPGHPAKGHQLA
                     EALVDAFKKPFTIDGIALFVGTSIGISAAPPDGTTPEQLMKASDVALYAAKKDGRGRAR
                     PFDPSMLELLEQRELLRRSLRMALVQQQFFIEYQPIAEGGCIVGFEALIRWHHPLLGKI
                     PPTAFIPMAEADGMMPEIGAWVLEQACREAMKWPQNYIVAVNLSAAEFLTSGLTDRVSQ
                     TLDLIGLPPDRLELEITESVLLERTVNNIDTLNTLNVLGIRISLDDFGTEYSSLSYLKT
                     FPFDTIKIDKYFITDLESDLKSQAIVRCIVNLAHDLDMQVTAEGVETPGQAEWLRSVGC
                     DRLQGYLISRPLPVQAIGEFITRADTSVSPALPQ"
     gene            8350..8859
                     /locus_tag="ELG62_RS14715"
                     /old_locus_tag="ELG62_14720"
     CDS             8350..8859
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517465.1"
                     /locus_tag="ELG62_RS14715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14720"
                     /product="hypothetical protein"
                     /protein_id="WP_041936384.1"
                     /transl_table=11
                     /translation="MEQEAASFDDHAFSPMVSLELNVAMFKSRWQFCDKITEYLSDILG
                     QGHSDPARYSNFLSVATNELIELAFRSTSELGRISFSLYRNATFNRLRITFPCEEASSR
                     EHTSARASEHQPVPGAGSGYVVMKSDGTVLLSDLAAIFRATIRIEKDGRQGIHIIADFP
                     SIEDFQ"
     gene            8856..10028
                     /locus_tag="ELG62_RS14720"
                     /old_locus_tag="ELG62_14725"
     CDS             8856..10028
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494684.1"
                     /locus_tag="ELG62_RS14720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ELG62_14725"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_130853100.1"
                     /transl_table=11
                     /translation="MSLLPLPFTVHFDPTNQELLLSGKMRPESAAEITDALKLVQQSLE
                     KYSGVVYVNVKRLTHINMTAFAALADILAASSRIRPERKIVIITSSVVAWSTSLFQTLA
                     ASQPSLSVEVYDSAFYPGQSFVEDETFIPILRTQTKMTWRHERELLPRHGLRSGLVIAD
                     ICCGIGDFAALVQREFKPARLVALDHSVRSLEYARRVAADFDLQGIEYTYGDASQMLLR
                     DNQFDFVTCRHSLQIFNRPEMLLRELYRICKPGGRVYITNEKNSHCLGEPHAESIKWTY
                     EEVAKLFKHFDMDVEMGPKSRHLLADAGFDNIKVDSFIVTNLDGDPQDFADIIEAWENV
                     YAGEMAVRRGDSSDFIRRFRQGFKDHVFAALHPKGYAGWPIWAASGRKPL"
ORIGIN
        1 taggcctgtt gcgcaacgaa ataatccgcc ttcgccatcc cgatcttgtt caagagcgcg
       61 aaggcgtgcg ctgcaggtgc tgcgagcgcc atgcccgtta tgatgatggc gaggaatctc
      121 atggtggata aaccggccgc gaggcgatgg gttcctgcag aaaatatgga acagtgtcgt
      181 ccgccatcgg ttgatcaaag gtccacctca aacatggagc cgaaaatgag caagaccaac
      241 gctcgtcaac ttcagaaacg cgccgaacag caggtgaaga ataccagcgc cggcggacat
      301 ctcggcggca catatttcgg gcagcaaccg gacggaaaaa ccgcctcgtc gaccaacaac
      361 gatagcgcca aggcgcggcc gaatgacgga agtaaatagg cgccgcgggc gcgcttcacc
      421 ccactccacc taagcactcc gcgcccggga acctgggcgc gcgcctcttc gcgggcgatc
      481 cacctcgcta ttgatcggcg acgcctgcgc gctagcttct gctcgcggcc gatcggccgc
      541 tggggatacg atctttggat gagggcggag gggcgacgtg gcatcctatt cgattgacga
      601 tgcgatccgg gaacttgccc cggctctcgg caaggcgccg gccggcgcgg tcagcggcga
      661 ttggaccgcc accacgatgc aggcggggca ttcttccagg acgggcggct acctcgatgc
      721 cgagggaaag catgtcccgg aggattccag gcatccgctc gacatcatcg cggatgtcgt
      781 cgaaaagctc gatgcatccg cgctgcagcg cttcaacaag gtggtgatcc gctggaagaa
      841 gccgaaattt ccgttcatgc aggcgaagat cacgctcgaa accagttatg accagacgat
      901 cgtgccgcgc ggccccgacg acccgatcta cgagactgcg gccgctacgc ggcgcgtctt
      961 ctggcagagc cgcggcacgc tgcacgaaga ctttgccgtc gagcgcggaa cggccaacat
     1021 tcacgcccag acgaaatggt tcggcccgca tcgccgcatc ctcgccatcc atgcgccgga
     1081 aaggctgata cttgccaccg acgggctgtc gaccccctgg gccggcatat ccgagccgga
     1141 aaacggcgtc gaatgcgagc ttttcatgga attcgatgcc gcgacactgg atgcagccgg
     1201 gatcgaaaac tgggcgaacc tgttgatcaa catcggagac ctcgtggcag acggctatcg
     1261 tgtcgcccgc gatgtcgaga agcacggcgc catcctgttc tgccggctga ccgaggatta
     1321 ccacccgatg acccgcatca tgctgagccg caaccccggg cgcatcgacc gcctgccttt
     1381 cggctccgtg ccgctgatcc gggcaacgcc gatcgccgaa accgaaatcg aagggcagga
     1441 cctttcctac gactggggcg ctgcggccgc ccgcaacgcc ctggccaaac gcggcttggg
     1501 gattgattga tgtcgccgat gccgggcggt tatcgtccgg ccggcacggt cctgcgctat
     1561 tcctggatgt cgggttcgac gaccctggcg aggtttcgca gtgactcctg ccagccgaga
     1621 tagcaagcct cgggcggaat gacgtcaggc acgcctgcct gcacgatatc cacctcggtg
     1681 ccgaccgaaa cctttttcag cgtcacggta acttccattt cgccgggcag gttggggtcc
     1741 tcgaatttgt cggtgtagcg caaacgttcg ccggggacga gctcgagata ttcgccgccg
     1801 aaggcatggc tattgcccgt cgtgaagttg cggaaggaca ttctgaacgt gccgccgaca
     1861 accggctccg aatgatgcac ggtgcagagg aagccgttcg gcggaagcca cttggcaagc
     1921 gcgtctgcct ccaggaatgc gcgatagact ttttccgggc tggtcgccag aacgcggtgc
     1981 aggcgtatgg tactcggcat ggtcgtgatc cttttgaatg gacggaatgg acggaatgga
     2041 cgcacctagg acgggcgagg catggccgat ccgacaccgg atcgaatttt tcttcaagaa
     2101 aaattgcctg gcgtagttca accgggcata ccgtcgaaca gcggaagatc gagcggcctt
     2161 gcgcaggtca gccggcgccg gatgaacatc tcgccccccg gctcgatctc ctcgcgcacc
     2221 gacgcggcaa ctctcagctt agctcaaaaa atggacgttt agtgcgacat ccattcgctg
     2281 ggctgagccg acagacgtct caagggcatt tgaaaagcct gaccggcttt cccggcccgc
     2341 gctggatgat gcgcttggct tccagatcca gttgaacggc cttcaaccac catccggccc
     2401 tagaacagga tgattttagg gcgggtcggc ctaaaatctg aatcctgttc tacgttatat
     2461 agttagagca tgatgtcgtc cgaaaaccgc tcacactttt cggcatcatg ctctagcgca
     2521 gccggggaat tcgatctcgg agagatgcgg caggatggcg atgtttgcct ctgctacggt
     2581 cagtccgggt gccccctcag gcagtgcggc cagcagagcg tggcgcattg ccatgtattt
     2641 ggccttgttg acccgctccg tatggtgcgg cgaggttatg ctttcaatga ggattttttc
     2701 gtcatcaggc atcgatcgct cctttgacag tgagtttggg tactttgaaa cccatcgcaa
     2761 tccacgcggt gagaagtctg ccatagaagc cggccgtcgc cagtgcgccg gaaggcacgt
     2821 catcgggcac actcgcgcga tcgggcacat tctgggtcat cgtgcgcaat tggaggggtg
     2881 gaagctcgtc ctgcttccag agattgccgt agaggctaag ccagtggccg cccttgaact
     2941 ccagaaaaat gggcgtgttg cagcaggacg ccaggacacg tcgcgttgaa gcattggggc
     3001 cgaggcggaa ttcccgcaga ttttcggttc cggccaaaag gctgatccga tccttccgat
     3061 aaagcacgta gcgcgtaccg ccatggatcg tccggatcgc ctccggcatc ggaagcgact
     3121 ccagcctgcc agccgcgtca ctacaactct tgcagtgaca ttccgcgctg atgatcgggg
     3181 cgccgcgcac ttcgaggtga aaggttccgc aggcacacgt cagatgggtt gtcttgttgt
     3241 tcatgacatt gctcgctgtt gaggatcgct gaatagccgc aaaatcaaac tagaccgttt
     3301 agttccgtta taagaactag acggttcagt ttgatcgtgt caagtagggt tggagcggag
     3361 atcgagaatg ttgataccgg gtatggaagc cgacctggcc gagcggcggg ccggcactcg
     3421 ttcggagcgc aaacaggcgg cgatcgtcgg tgccgcggcc gaagtctttc tgagcaccgg
     3481 ttatgccgga gccagcatgg acgaaatcgc cagccggtcc ggcgtatcca agcagacggt
     3541 ctacaagcac ttctcaagca aggaggcgct gttcgtcgcg gtgctcaccc agatgatggg
     3601 agaggcagat accgccgttc acaccggcct cccccaggtg gagaaccgcg ctcagctgga
     3661 agcctatctc cttgactacg ccgtccgcca actcaccatc gtcctgacgc cggggctgat
     3721 gcaattgcgc cggctcgtga ttgcggaggc gcaacgcttc ccggaactcg ccaagatgct
     3781 ttacgcgcgc ggccctgcac gggcgctcga ggtcatgggc agcgctttcg aacagctagc
     3841 cggcaagaag ctgctgcagt tctccgatgc aatggtagcc gcatcacagt tcaactggct
     3901 ggtgatggcc gaccccgtca accgggtcat gatgctgggc gatgcggcca ttccgaccaa
     3961 gcagcagatt taccgccacg ccgaggcggc cgtcgccacg ttcctggcgg catttctgca
     4021 tccagagaag cgctgaacgc cgtcgaaggt ccgtgcctca cagcggcggt gactggaatt
     4081 ttccgcacgt gccaacaggc cggcggtcac aaagccgccg ctcaatcgtc agcaaatcgc
     4141 cccgttaccg ccgcaccgtg ccctcttaaa ggttccatcg tcgcgacctc gcccggctct
     4201 tgagtggctg agcgatcgac cgaggcggtc ttgctgccaa tcaaatcatg cacttgaaga
     4261 ggaatgaata tgatcactcg ttacacatct gtcgcagcac tgagcgcgac agtcttcagt
     4321 ctgcttgctt tcagcccggc atccgctgtc gacatggccc aggcgcaaca gcagccggca
     4381 caagcgcaac cgccggcgca aggccaaaca ccgatgcagg ggcagaccgg cggcaacggt
     4441 acggccgccc ccgtcagcga tcagaaaatc gaggccttcg ccgttgccta tctgcaggtc
     4501 gacaaggtga gacaggaata ttcggccaag atcggcgcga cgaaggacga ggccgcgaag
     4561 caacagctgc aggaagaggc caaaaagcag atggtcgata ccgtccaagc ttctcccgac
     4621 atctccgtcg aagagtactc gtcgatcctg acggcggcgc agagcgaccc ggctctggcc
     4681 aagaaggttc tggaaaagat cggcacgccg ccgctgggcc agcagcagcc acagcagggc
     4741 cagcagcagc cgcagcaata ggctacgcgg gcgtagtaag tcgccacgaa taggcagcgg
     4801 acgtcttgcc gttcgcgccg gaagcagcgc ccgccatttg ccgggctctg ctttccacct
     4861 aagaagtcga cgaaatgatc ggccagaaca ggatgatttt aggccgggta ggcctaaaat
     4921 ctgaatcctg ttctacattc tatagttaga gcatgatttc gtccgaaaac cgctcacact
     4981 tttcggcatc atgctctggt tcagtactgc gacccgctct tattgttcag gtcgtgccgt
     5041 tcggtgcccg tcagcggcgg gttgaagatg ctgacgagaa cgagatcctg atccttgccg
     5101 ccgcgcagat aatggcggtc gtgcttgtcg agcacgtaga tatcgccggc gcggatcgaa
     5161 aagacattgc cgtccatatc ctccacttcg ccctcgccct tgatgcagta gcatgcctcc
     5221 agatgattgc ggtattgaag caaggcctcg ctattggcgc gaacgacagt atggcacacc
     5281 gtaaagccca tgccgtcgtc tttggtcagc aggcggtggc ttgtgccgtt gccccaatcg
     5341 acgaaacgct cggtgagctc tacctcctta agatttctga cgaacacgtc aattcccctg
     5401 ataaattatt gaagatgcag tgatgattca ttttcatcgc ccgcaacaat tagctattga
     5461 aatcactggc attttcaaat gatgatttct gcgtcatctg tgaggaaatt ttacaaatgc
     5521 aattgccgcc gttaaacgct ttgcgcgctt tcgaagccac ggcccgcctg atgagccttt
     5581 cgaaagcggg cgacgagctg cacgtcactc atgcagcgat cagccatcag atcaagcatc
     5641 tggaaacctg gctcggccgc aagctgctgc agaaatcggg ccggggcatt acgctgaccg
     5701 cagccggcgg cgaatattac cgggcagtct caggatcgct ggcggcgatc gcacatgcga
     5761 ccggcaatat gcgacgcgac cacgacatgc gcgccatcac cgtcggctgc attccctcga
     5821 tcgcatctcg ctggctcgtc ccggccctgc cctccttcca ggagagtgac cccgatctcg
     5881 acgtcaggat cgtctatgcc cgcgccgaag aacgcttcga cgacgagagc ctcgacgtgc
     5941 tgatcaccat gggcgaggat ctgagcggcg gcagggaaag ccgcctgctc ttctcccgcc
     6001 gcaaccagcc tgtcgccagc cgccattacc ttgccaaacg caactggacg atcggcgacg
     6061 tctcgctcgc cggcgccgat ctgctgcacg acgaatccgt cgatggttgg aaagaatggt
     6121 tccgcaaggc cggccagaaa gcgccggagc cgctccgagg cccgatgttc caggatttca
     6181 acatgctggc gacggccgta atcgccggcc atggcgtggc gctctgccct gtcgaggtgt
     6241 tccgccgcga gatcgaacgc ggcgatctgc tggtgctttc cgacatcgcg acggcagaga
     6301 accagggcta ttacgtcata tcgaacagct gggcgaaaaa gccggtccgc cgcttcatcg
     6361 actggttcat gacagagtgc ggatcgggcg catgagatcc cctgcccgct gctcatcgaa
     6421 gggtcgccgc cgagacgatg gatgtaaatg ggtcgcggca ataaagacgt tgcataagcc
     6481 catccgagcg cgagttgggc agaagttggg gattgttaat cagaaatgcc taatgtaaat
     6541 cttggcgtgc acaagttgtg cgggagctga tttgctacaa tatctggtta tgacatgcga
     6601 ctagggaggg cctcggacat gcggaaaatt catgcggccc cctcggcgct cggacaaaca
     6661 gaggcaaaag cttcgagctc gatcgaccgg cttttgttct ttgaccgcga tttcaatccc
     6721 gtcgagaatt tgctgggcaa tgagctgcct gcttctgtca aaccagctgc gtcgttttgg
     6781 gagcattttc cagaattgga caaatcggcg attacgctcg ccctccgttc attgggtgac
     6841 agtgctcagc ccttgcaaat atcgggggat cttggcgcgc cagggtcgca cggactgacg
     6901 atctatcgga tcggagatct gtttgccgtg gttcggtccg aggaatctat cgacgatgag
     6961 cgcgcaaccc ttttgcattt ggccacccac gatccgctca ctggactgcc gaaccgacgc
     7021 cagttcagcg aggaccttgc tgtgttgtta cgggagaccg caggctcgga tgagactctg
     7081 tccctgatgc aactcgatct tgacgatttc aaacctgtta acgacacgct tgggcatccg
     7141 gccggcgaca agcttcttca gctcgctgca agccgcattc aagagtgcct taccagggac
     7201 gacagagcct atcgactggc gggtgacgaa ttcaccgtca tatcaagggg cccgggacat
     7261 cccgccaaag ggcaccagct agcggaagct ttggttgatg cattcaaaaa gcccttcacg
     7321 atagatggca tcgcgctatt tgtcggtacc agcatcggca tatctgctgc tccaccggac
     7381 ggtacaactc cggagcagct gatgaaggcg tccgacgtcg cactctacgc cgcgaagaag
     7441 gacgggcgcg gtcgcgcaag gccgtttgat ccctcaatgc ttgagttgct ggaacaacgc
     7501 gaactgctgc gccgcagcct tcgcatggcc ttggtccaac aacaattttt tattgagtac
     7561 caacccatcg ccgagggcgg ctgcattgtc ggcttcgaag cattgatcag atggcaccac
     7621 cccctccttg gaaaaatccc gccaacggca tttattccga tggccgaggc cgatggaatg
     7681 atgccggaaa taggtgcatg ggttttggag caggcgtgtc gcgaggcaat gaagtggccg
     7741 cagaactata tcgtcgctgt caatttgtca gcggctgaat tcttgacaag cggcctcacc
     7801 gatagggtct cccagacgct ggacttgatc gggttgccgc ccgaccgtct ggagcttgag
     7861 ataaccgaaa gcgtgctgct tgagcggacc gtcaacaata tcgacaccct gaatactctg
     7921 aacgtgctag ggatacgaat ctcgcttgat gatttcggga ccgaatattc ttcgctgagc
     7981 tatctgaaga cattcccgtt cgacacgatc aaaatcgaca aatactttat caccgacctc
     8041 gaaagcgatc tgaaaagcca ggcaatcgtc cgctgcatcg ttaaccttgc acatgacctc
     8101 gacatgcagg tgacagccga aggggtagag acgccaggtc aggcggaatg gttgcgcagt
     8161 gtgggctgcg acagacttca aggctatttg atcagcaggc cgcttccggt ccaggcgatt
     8221 ggcgagttca tcacccgggc ggacacatcc gtaagcccag cgctaccaca gtgaacaacc
     8281 gattttcgcc gtgcctttag ctatcgcatg catcaacctg acgccccagg caaaagaagg
     8341 gagggtttat tggaacagga agcagctagc ttcgatgacc atgcattttc gccgatggta
     8401 tcgctggaac tgaacgttgc gatgttcaaa tcccgatggc agttttgcga caagattacg
     8461 gaatatctct cagacattct cggccaaggt cacagcgatc ccgctcgata ttccaatttt
     8521 ctttccgtcg cgacgaacga gctcatcgaa cttgccttcc gttcgacgag cgagctggga
     8581 aggatcagtt tcagtctcta ccggaatgcg acattcaatc ggctgagaat tacgtttccg
     8641 tgcgaggaag catctagccg agagcacacg agtgcaagag caagcgaaca ccagcctgtc
     8701 cccggcgccg gctctggata cgtggtgatg aagtccgacg ggacggtttt gcttagtgat
     8761 ttggcagcaa tattccgcgc cacgatccga attgagaaag acggcagaca agggattcac
     8821 atcattgcgg atttcccctc gatcgaggac ttccaatgag cctgcttccc ctacccttca
     8881 ctgttcattt tgatccgaca aatcaggagc tgttgctgtc tggcaagatg cgccccgaaa
     8941 gcgccgctga aatcaccgac gccctcaagc tggtgcagca aagtttggaa aaatacagcg
     9001 gggtcgttta cgtcaatgtc aaacgtctga cgcacatcaa catgactgcc ttcgcggccc
     9061 tggctgatat actcgcggca agttcccgga tcagaccgga acggaagatc gtcatcatta
     9121 cgtcgagcgt ggtggcatgg tcgacctcgt tattccaaac gctggcggca tcacaaccaa
     9181 gcctgtcggt tgaagtctac gattcggcgt tttatccggg gcagagcttt gtcgaggatg
     9241 agactttcat tcccattctg cgtacccaga cgaaaatgac atggagacac gaacgcgaac
     9301 tgttgcctcg ccacggcctg cgcagcggtc ttgtcatcgc agatatctgt tgcggaattg
     9361 gtgatttcgc cgccctggtt caacgggaat ttaaaccggc acgccttgtc gcgctggatc
     9421 attccgtgcg cagcctcgaa tatgcgcgca gggtcgcggc cgacttcgat ctgcagggaa
     9481 tcgagtacac ctacggtgac gcttcccaaa tgctgctgcg agacaatcaa tttgactttg
     9541 tcacctgccg acattcgctg cagattttca accgtcccga gatgctgctc agggagcttt
     9601 accgcatctg caagcctggc ggccgtgtct acatcaccaa cgaaaaaaac tcgcattgtc
     9661 tgggcgagcc acatgctgaa tccataaaat ggacttacga agaagtcgcc aaactgttca
     9721 agcacttcga catggatgtc gagatggggc cgaaaagccg ccacctcctc gccgacgcgg
     9781 gcttcgataa catcaaggtc gattccttta tagtcacgaa tcttgacggg gatccccagg
     9841 acttcgcaga catcatcgaa gcttgggaaa atgtctacgc gggtgagatg gccgttcgac
     9901 ggggcgactc atcagatttc attcgtaggt tccggcaagg attcaaagat catgtctttg
     9961 ctgctcttca ccccaagggt tatgcaggct ggccgatctg ggcagcttcc ggccgaaagc
    10021 cactgtgatg gacagaagct catcgaaaag accggcaaga ctaggttcgt ttcgggcaaa
    10081 gttcatcttg gttgtcggtg gagctgtcct ctttgatctg ctggtcagcg gcggactggc
    10141 actttggaat gttcagaggc tgtcgcgcga cgccaccctc gaagtcggac agggtctgga
    10201 agcagcaagc caggaataca tccgcacata tgccgattcc accgcagccc aggtgagttt
    10261 gctcctgcgg caggttcata gcgacgtcgg caccctggca ggtgtgctcc aggcgcaaat
    10321 cgacgatccc gagcgtaatt ccgatgtcgg cgcggcgatc gcgcgcacat cgcctggtag
    10381 cgtcagc
//
