LOCUS       NZ_CP036170            21658 bp    DNA     circular CON 15-DEC-2024
DEFINITION  [Clostridium] scindens ATCC 35704 chromosome, complete genome.
ACCESSION   NZ_CP036170
VERSION     NZ_CP036170.1
KEYWORDS    RefSeq.
SOURCE      [Clostridium] scindens ATCC 35704
  ORGANISM  [Clostridium] scindens ATCC 35704
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3658040)
  AUTHORS   Devendran,S., Shrestha,R., Alves,J.M.P., Wolf,P.G., Ly,L.,
            Hernandez,A.G., Mendez-Garcia,C., Inboden,A., Wiley,J., Paul,O.,
            Allen,A., Springer,E., Wright,C.L., Fields,C.J., Daniel,S.L. and
            Ridlon,J.M.
  TITLE     Clostridium scindens ATCC 35704: Integration of Nutritional
            Requirements, the Complete Genome Sequence, and Global
            Transcriptional Responses to Bile Acids
  JOURNAL   Appl Environ Microbiol 85 (7), e00052-19 (2019)
   PUBMED   30737348
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658040)
  AUTHORS   Devendran,S. and Ridlon,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2019) Dept. of Parasitology, University of Sao
            Paulo, Av. Prof. Lineu Prestes, 1374, Sao Paulo, SP 05508000, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004295125.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 14:32:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,771
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,591
            CDSs (with protein)               :: 3,591
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 30 of 106
            Pseudo Genes (incomplete)         :: 86 of 106
            Pseudo Genes (internal stop)      :: 13 of 106
            Pseudo Genes (multiple problems)  :: 19 of 106
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:01:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3338912
            Orig. end                         :: 3360570
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036170.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21658
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ranthipeptide"
                     /region_number="4"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /tool="antismash"
     cand_cluster    1..21658
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /kind="single"
                     /product="ranthipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21658
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11658](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11658)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
     gene            complement(851..2308)
                     /db_xref="GeneID:62697593"
                     /gene="hemZ"
                     /locus_tag="HDCHBGLK_RS17140"
                     /old_locus_tag="HDCHBGLK_03413"
     CDS             complement(851..2308)
                     /EC_number="1.3.98.3"
                     /GO_function="GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697593"
                     /gene="hemZ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331580.1"
                     /locus_tag="HDCHBGLK_RS17140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03413"
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="WP_009248620.1"
                     /sec_met_domain="PF04055 (E-value: 7.6e-21, bitscore: 73.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRIICNSEAYTYNTYHMIKAFYPAAQAVCRVEEKASNYVTVLLE
                     NGRKITTGAADMPAEYSGIGKEAEVKRHIDVKLYHLLEEETGNSLSWGILTGVRPTKIA
                     MKKLDEGMDEKAFVSWFHEIYMVSKEKASLAWEIAGREKELLERLDYDNGYSLYVGIPF
                     CPTVCSYCSFSSGALADWESRVEDYLDALCKELKFIAQKSSLKKLNTIYIGGGTPTTLT
                     AGQLERLLCCIDENFSREHLLEYTVEAGRPDSITREKLEAIRRHGVTRISINPQSMQQK
                     TLDAIGRRHSVEQIITAYGMARELGFDNINMDIIAGLPGEDLHDMEDTLRQIGRLMPDS
                     LTVHSLAIKRAARMDMEDLKREDGKTNEVMSGMIRSAERLARQMGLAPYYLYRQKNIAG
                     NFENVGYAKVDKAGIYNILIMEEKQSIVAAGAGASTKIVLKGIIPMPGSRKKKMTRLMR
                     IENVKAIDAYIDRIDEMIERKGEWLWR"
     gene            complement(2321..2941)
                     /db_xref="GeneID:62697594"
                     /locus_tag="HDCHBGLK_RS17145"
                     /old_locus_tag="HDCHBGLK_03414"
     CDS             complement(2321..2941)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697594"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605091.1"
                     /locus_tag="HDCHBGLK_RS17145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03414"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_004605091.1"
                     /transl_table=11
                     /translation="MKVERFVTGIISTNCYLAVNEETRQAVVVDPAACPSYLLSHIKSE
                     GIKIEALLLTHAHFDHIMGIDGFVDEFHVPVYVYEEDADAMTDPKLNQSSVYTGGYTYE
                     GAKSIKEGQILKLAGYDFEVFHTPGHTKGGCCYYVRSEGVLFSGDTLFQNSIGRTDFPG
                     GSMSDLVRAVKEKLMVLPDETLVYPGHMGETTIGHEKTHNPFL"
     gene            complement(2947..5292)
                     /db_xref="GeneID:62697595"
                     /locus_tag="HDCHBGLK_RS17150"
                     /old_locus_tag="HDCHBGLK_03415"
     CDS             complement(2947..5292)
                     /EC_number="2.7.6.5"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248621.1"
                     /locus_tag="HDCHBGLK_RS17150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03415"
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="WP_004605090.1"
                     /transl_table=11
                     /translation="MSEKTTYEAIDGRIAPESLTADASAGLEVVDGHAVKAPGDYENPD
                     RLYEMLIARIRRYHPSTDVSMIEKAYQLAKKAHGGQCRKSGEPYIVHPLWVAIILADLE
                     MDKETIVSGMLHDVVEDTEVGEEDIKREFGEEVALLVDGVTKLGRLSYSSDKLEVQAEN
                     LRKMFLAMAKDIRVIIIKLADRLHNMRTLEFMTPAKQKEKAKETMDIYAPIAQRLGISK
                     IKTELDDLALKYSQPEVFFDLVNQINARKTEREEFVQQIVDEVSTHMKNASIKADVNGR
                     VKHFFSIYKKMVNQDKTVDQVYDLFAVRIIVDSVKDCYAALGVIHEMYTPIPGRFKDYI
                     AMPKPNMYQSLHTTLMSSVGQPFEIQIRTQEMHKTAEYGIAAHWKYKESNDGKKSVEAQ
                     EEEKLSWLRQILEWQRDMSDNREFLSLIKGDLDLFAEDVYCFTPQGDVKNLPNGSTPID
                     FAYAIHSAVGNKMVGARVNGKLVNIEYKIQNGDRIEILTSQNSRGPSRDWLGIVKSTQA
                     KNKINQWFKKEFKESNIIKGKEMIASYCKAKSINLSNIMNPKYQDVVQKKYGFKDWEAV
                     LAAIGHGGLKEGQVVNRLVEEYDKEHKQEITDEVVLERVAEASKNKVHIAKSKSGIVVK
                     GIDDMAVRFSRCCNPVPGDEIVGFVTRGRGLSIHRTDCVNMIHLTESERARLIDAEWES
                     DVAEKAGGQYLAEIKMYAHDRQGLLMEMSKIFTEGDVDVKSMNVRTSKQGTATIETGFI
                     VHGREELTRIVKKLRQVEGVIDIERTTG"
     gene            complement(5332..5856)
                     /db_xref="GeneID:62697596"
                     /locus_tag="HDCHBGLK_RS17155"
                     /old_locus_tag="HDCHBGLK_03416"
     CDS             complement(5332..5856)
                     /EC_number="2.4.2.7"
                     /GO_function="GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006168 - adenine salvage [Evidence IEA]"
                     /GO_process="GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427080.1"
                     /locus_tag="HDCHBGLK_RS17155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03416"
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="WP_004605089.1"
                     /transl_table=11
                     /translation="MKPIEEYVRSIPDFPESGIIFRDVTSVLQDADGLMLAIDLMQEKL
                     KDVEFDVVVGPESRGFIFGVPIAYNLHKPFIPIRKKGKLPCETVSIKYELEYGIAELEM
                     HRDAIKPGQKVVIIDDLIATGGTNEAMIKLVEGLGGEVVKTVFLMELEGLKGRDKLKGY
                     DVESVIAYPGK"
     misc_feature    5912..5926
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.79"
                     /tool="antismash"
     gene            complement(5946..7706)
                     /db_xref="GeneID:62697597"
                     /gene="recJ"
                     /locus_tag="HDCHBGLK_RS17160"
                     /old_locus_tag="HDCHBGLK_03417"
     CDS             complement(5946..7706)
                     /GO_function="GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697597"
                     /gene="recJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440042.1"
                     /locus_tag="HDCHBGLK_RS17160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03417"
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="WP_004605088.1"
                     /transl_table=11
                     /translation="MEQWVLLRKGADFEGIGKRFQISPRLACLIRNRDVIGDEAIAQYL
                     NGTIADLYDGMLMKDMDKAVDILREKIAEQKRIRVIGDYDIDGVNATYILLEGLEKLGA
                     EADSDIPSRMKDGYGLNIELIERAYQDGIDTIITCDNGIAAAEEIACGKRLGMTIVVTD
                     HHEVPFEEEEDGKKYLLPPADAVIDPKQPGCEYPFKGLCGAAIAYKLVEALWEATGGDA
                     EDLDYLIENVAIATVGDVMELENENRIFVKEGLQMLKRTHSPGLRSLIACTGVDKDRIG
                     AYHIGFVLGPCMNASGRLDTAKRTLNLLRAKSGKEADILAGDLKALNDSRKEMTDIAVE
                     QARQIVDTTKAGKDRVLVLYLPGCHESLAGIVAGRIRERYYRPAIILTDAEEGIKGSGR
                     SIEAYNMYEELSRCRDLLTKFGGHKLAAGMSLAKENIEELRRTLNDNCRLEPKDMAEKV
                     VIDMELPFSCVTEELVEELTLLEPFGKGNTKPVFAARNVELISGRILGKNRNVLKLQVK
                     DSSQTAREAVCFRGAEQMLSLLEERYGKEAVDLLMKGRGGQMKLSVTYYPDINEYMGKR
                     TVQIVITHYQ"
     gene            complement(7791..9965)
                     /db_xref="GeneID:62697598"
                     /locus_tag="HDCHBGLK_RS17165"
                     /old_locus_tag="HDCHBGLK_03418"
     CDS             complement(7791..9965)
                     /codon_start=1
                     /db_xref="GeneID:62697598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605087.1"
                     /locus_tag="HDCHBGLK_RS17165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03418"
                     /product="protein translocase subunit SecDF"
                     /protein_id="WP_004605087.1"
                     /transl_table=11
                     /translation="MKKNKGILSLILTVVLVVLLGFTVLVGFGKTGTGAMKNIKLGLDL
                     AGGVSITYQVKDDNPTEKEMSDTIYKLQKRVEQYSTEASVYQEGDDRINIEIPGVTDAN
                     AILDELGKPGSLEFRTEDGETVITGSDVKTATAKAGEDDMGNKEYSVELSLNKEGTKKF
                     ADATEANVGKTISIIYDGEAISSPRVQTAITGGQAYITGNFSYDEAENLASTIRIGGLK
                     LELEELRSNVVGAQLGEQAISTSLKAGAIGLAIVFIFMIFVYYLPGLASSLALVIYTEL
                     VLLILNAFNVTLTLPGIAGIILSIGMAVDANVIIFARVREEMSRGKSVKNALKTGFQKA
                     MSAIVDGNITTLIAAAVLWFKGSGTVKGFAQTLAIGIIVSMFTALVITRMIVYAFYAVG
                     LRKEKLYYRAKKERKTINFLGKKKWFFTVSTAVIAAGLIFMGVNSSKGNGAFAYSLEFE
                     GGTSTNVTFNEDYTIEEIDKEIVPVVEKVTGDHNVQTQKVAGTNQVIIKTVTLELDQRE
                     KLNQAMADNFGVDESKITAENISSTVSNEMRQDAVVAVIVATIFMLLYIWFRFKDIRFA
                     TSAVTALLHDVLVVLAFYAVARISVGNTFIACMLTIVGYSINATIVIFDRIREELHYQT
                     KSTDLAEVVNKSITMTLTRSIYTSLTTFIMVAVLYVMGVSSIREFALPLIVGIVCGAYS
                     SVCITGALWYVMKTKTGKKPAAQTSKKKKK"
     gene            complement(10001..11395)
                     /db_xref="GeneID:62697599"
                     /gene="scfB"
                     /locus_tag="HDCHBGLK_RS17170"
                     /old_locus_tag="HDCHBGLK_03419"
     CDS             complement(10001..11395)
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697599"
                     /gene="scfB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Ranthipeptide_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427083.1"
                     /locus_tag="HDCHBGLK_RS17170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03419"
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="WP_039909278.1"
                     /sec_met_domain="Ranthipeptide_rSAM_RRE (E-value: 1.8e-29,
                     bitscore: 99.7, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="PTHR43273 (E-value: 4e-75, bitscore:
                     250.9, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 1.4e-15, bitscore: 55.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 6.5e-13, bitscore: 47.2,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 4.4e-18, bitscore:
                     63.2, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIHQYQNNGYNIVMDVYSGAVHVVDELCYDVIAKLSKENEAHTAK
                     TLKEPCVLKGLKKELGQKYEEGEIEDALADVIELTEAGRLFAEDAYECMIEEVKKRKTV
                     VKALCLHIAHDCNLACKYCFAEEGEYHGRRALMSFEVGKKALDFLIANSGNRHNLEVDF
                     FGGEPLMNWQVVKDLVAYGREQEKIHDKHFRFTITTNGVLLNDEVQEFVNKEMDNVVLS
                     LDGRKDVNDRMRPFRNGKGSYDLIVPKFQKLAKSRNQERYYVRGTFTRDNLDFSNDILH
                     FADLGFKQMSIEPVVGEESDPYAIREEDIPVINEEYDKLAKIMIEREKEGKGFNFFHFM
                     IDLDGGPCVAKRLSGCGSGTEYLAVTPWGDLYPCHQFVGQVEFLMGNVDEGIVRPEIAD
                     DFRSCNVYSKDKCRDCFAKFYCSGGCMANSYNFHGTIHDTYDIGCEMQRKRVECAIMMK
                     AALAEE"
     aSDomain        complement(11063..11395)
                     /aSDomain="Ranthipeptide_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a
                     ranthipeptide/SCIFF cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_HDCHBGLK_RS17170_Ranthipeptide_rSAM_R
                     RE.1"
                     /evalue="1.30E-31"
                     /identifier="RREFam024.1"
                     /label="HDCHBGLK_RS17170"
                     /locus_tag="HDCHBGLK_RS17170"
                     /protein_end="111"
                     /protein_start="0"
                     /score="99.7"
                     /tool="antismash"
                     /translation="MIHQYQNNGYNIVMDVYSGAVHVVDELCYDVIAKLSKENEAHTAK
                     TLKEPCVLKGLKKELGQKYEEGEIEDALADVIELTEAGRLFAEDAYECMIEEVKKRKTV
                     VKALCLH"
     misc_feature    11361..11379
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.59"
                     /tool="antismash"
     gene            complement(11515..11658)
                     /db_xref="GeneID:62697600"
                     /gene="scfA"
                     /locus_tag="HDCHBGLK_RS17175"
                     /old_locus_tag="HDCHBGLK_03420"
     CDS             complement(11515..11658)
                     /codon_start=1
                     /db_xref="GeneID:62697600"
                     /gene="scfA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: SCIFF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR03973"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605085.1"
                     /locus_tag="HDCHBGLK_RS17175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03420"
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="WP_004605085.1"
                     /sec_met_domain="TIGR03973 (E-value: 1.9e-24, bitscore:
                     83.0, seeds: 29, tool: rule-based-clusters)"
                     /sec_met_domain="SCIFF (E-value: 3.7e-24, bitscore: 82.2,
                     seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKHVKTLNTQTMNSNLKKSGCGECQTSCQSACKTSCTVGNQTCEQ
                     KK"
     gene            11897..12916
                     /db_xref="GeneID:62697601"
                     /gene="gap"
                     /locus_tag="HDCHBGLK_RS17180"
                     /old_locus_tag="HDCHBGLK_03421"
     CDS             11897..12916
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697601"
                     /gene="gap"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605084.1"
                     /locus_tag="HDCHBGLK_RS17180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03421"
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="WP_004605084.1"
                     /transl_table=11
                     /translation="MAIQIGINGFGRIGRVAFRIAISQPERFHLCGINVRNANLDYMVY
                     MIRYDTIFGRFRGELGTYEKGITVNGKKIPVFSESDASCIPWESCGAEYIIDATGAFCT
                     TAKAMSHIKAGAKKVIISAPAKDDETPTFVMGINHTNYTPDMKVISNASCTTNCLAPIC
                     KVLEDNYGIEYGLMSTIHAATAKQKVVDSRSLKDWRTGRAVFGNLIPSTTGAAKAISLV
                     IPSLKDRMNGISYRVPTSDVSIVDLNVALKSPSSYEDICSKVHEASMTYLSGILDYVDD
                     EVVSSDFIGDSHASIFDAREGIQVNSHFFKLICFYDNEWGYTSQIFRLIEYMDQIHQQ"
     gene            complement(13067..13441)
                     /db_xref="GeneID:62697602"
                     /locus_tag="HDCHBGLK_RS17185"
                     /old_locus_tag="HDCHBGLK_03422"
     CDS             complement(13067..13441)
                     /codon_start=1
                     /db_xref="GeneID:62697602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605083.1"
                     /locus_tag="HDCHBGLK_RS17185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03422"
                     /product="TIGR04086 family membrane protein"
                     /protein_id="WP_004605083.1"
                     /transl_table=11
                     /translation="MERQVRKDSKVMWVLKALLASYIVTGILLLLLTMALYKMELNEKM
                     VSAAIVAIYVMATLIGGILIGKMAKVKRFIWGLGLGIAYFALLLLITLGVYHTLNGDGA
                     NLVTTFILCAGGGMVGGMIS"
     gene            complement(13495..14043)
                     /db_xref="GeneID:62697603"
                     /gene="spoVT"
                     /locus_tag="HDCHBGLK_RS17190"
                     /old_locus_tag="HDCHBGLK_03423"
     CDS             complement(13495..14043)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697603"
                     /gene="spoVT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605082.1"
                     /locus_tag="HDCHBGLK_RS17190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03423"
                     /product="stage V sporulation protein T"
                     /protein_id="WP_004605082.1"
                     /transl_table=11
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDREGEII
                     LKKYSPIGELSAFAKQYAESLAQTMGCLVCVCDMDQIIAAAGTGKKDLQDKYISRPLEK
                     QLGDRNQIMAALGDKKYVAITDSQEEEYEYEIICPIICEGDVIGGVILLSKDAKKKLGE
                     LEQKMAACAAGFLGKQMEQ"
     gene            14222..14668
                     /db_xref="GeneID:62697604"
                     /locus_tag="HDCHBGLK_RS17195"
                     /old_locus_tag="HDCHBGLK_03424"
     CDS             14222..14668
                     /codon_start=1
                     /db_xref="GeneID:62697604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248622.1"
                     /locus_tag="HDCHBGLK_RS17195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03424"
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="WP_009248622.1"
                     /transl_table=11
                     /translation="MSKIEEVRGAMVKAMKAGDKETKETLSMLLAALKNKAIDKREDLT
                     EAEEIQVILKEIKQTKETLDMTPADRTEIIDECNKRLSVLEQYAPKMMDESEIKDVIEA
                     TLKEIGLDAPTAKDKGRIMKVLMPKVKGMADGKLVNELLMTYMG"
     gene            complement(14902..15972)
                     /db_xref="GeneID:62697605"
                     /locus_tag="HDCHBGLK_RS17200"
                     /old_locus_tag="HDCHBGLK_03425"
     CDS             complement(14902..15972)
                     /GO_function="GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605079.1"
                     /locus_tag="HDCHBGLK_RS17200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03425"
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="WP_004605079.1"
                     /transl_table=11
                     /translation="MKKKILILAMAGMLAATSLTGCGSFKDDDVVAVVGDKEITADIAN
                     FYARYTQAQYETYYAGYLGEDMWNSEAMEGQTYEESVKDSVLKQLETMILLEEHMGDYE
                     VSLSDKEKNAIESTAKEFDEANALEDKEKISGSLKTVKRVMTLMAIQQKMTEAIQADAD
                     TEVSDEEAAQKSMQYVLFPYTTKDADGKSVDLSDEEKAKVKSKAEDFAEAAKTAEDFGA
                     FATEQGVEASTITFDKDSSTPSADLIAAADKLGEGEVTEMIEAENGCYVGKVTSLFDRE
                     ATDTKKQSIVNDRKTDLYTKTCEKWLKKADIKVNKSVWKKVDFNDLSVTMKVEDQEPYA
                     NDVKTDDQVDTDSEGQ"
     gene            complement(16024..19368)
                     /db_xref="GeneID:62697606"
                     /gene="mfd"
                     /locus_tag="HDCHBGLK_RS17205"
                     /old_locus_tag="HDCHBGLK_03426"
     CDS             complement(16024..19368)
                     /GO_function="GO:0003684 - damaged DNA binding [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697606"
                     /gene="mfd"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605078.1"
                     /locus_tag="HDCHBGLK_RS17205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03426"
                     /product="transcription-repair coupling factor"
                     /protein_id="WP_004605078.1"
                     /transl_table=11
                     /translation="MQALIRPLNELAEFEEISRDIRKGAGMIRVCGCVNSQKTHMMYAL
                     SDGCNYRVIACSSESKAKQVYEEYRFLDPNTHLYPAKDLLFYQADLRSKELVSQRMEVI
                     QAVAAGEKATVITSFDAFMDTLLPKDVIVEKTVRIANDSTFHLEEMQSRLVALGYDREV
                     QIEAPGQFAVRGGILDVYPLTEELPIRIELWGDEVDSIRTFDVETQRSIENLEEVSICP
                     AVEFPQEGEKGVSFLDYFPMEETILFLDEPVRLIEKGQGVEEEFLEAQKKRVESGYEVT
                     DAEVQLYHTEEILRKMNAYSSVGFFALDMKCRGLETKASFSLQTKSVNPYNSSFDMLTQ
                     DLKRLKRNGYRVVLLSGSRTRAKRLAEDLRDYNLSSYYSDELDREVAPGEIMTAYGHVA
                     YGYEYPMLKFTVISETDIFGKTKKKKKRKTYEGRKIQSFAELKVGDYVVHENHGLGIYQ
                     GIEKIEVDKISKDYMKISYAQGGNLYIPATQLDLIQKYASADAKKPKLNRLGTQEWTKT
                     KTRVRGAVREIAKDLVRLYAARQEQEGYVYGEDTVWQREFEEMFPFEETEDQLLAIEAV
                     KQDMMSHKIMDRLICGDVGYGKTEIAIRAAFKAVQEDKQVVYLVPTTILAQQHYNTFAQ
                     RMKDFPVRVDLMCRFRTPAQQKKTIEDTKKGLVDIVIGTHRVLSDDLKFKDLGLLIIDE
                     EQRFGVQHKEKIKKLKENVDVLTLTATPIPRTLHMSLIGIRDMSVLEEAPNDRMPIQTY
                     VMEYNDEMVREAIERESGRQGQVYYVYNRVEDIAEITGHIQKLVPDVTVEYAHGQMKEH
                     QLERIMYDFINGEIDVLVSTTIIETGLDISNVNTMIIHDADHLGLSQLYQLRGRVGRSN
                     RMAYAFLLYRRDKLLREVAEKRLAAIREFTDLGSGFKIAMRDLEIRGAGNLLGAEQHGH
                     MEAVGYDLYCKMLNEAVKHLKGEMEEETFNTTMDLNVDAYIPDSYIPNEYQKLDIYKRV
                     AAIENEEEMEDMLEELIDRFGDIPKKVETLLAVASLKAIAHSAYVTAVEQKGERFTFSM
                     YEKAKVQPQKIPGLLEQFKGDLAFKADAENPCFLYEKKSRNKKEKNTDVLAVVKNVLIG
                     IKGLIDQ"
     gene            complement(19372..19956)
                     /db_xref="GeneID:62697607"
                     /gene="pth"
                     /locus_tag="HDCHBGLK_RS17210"
                     /old_locus_tag="HDCHBGLK_03427"
     CDS             complement(19372..19956)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697607"
                     /gene="pth"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605077.1"
                     /locus_tag="HDCHBGLK_RS17210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03427"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_004605077.1"
                     /transl_table=11
                     /translation="MFIIAGLGNPTLQYEGTRHNAGFDVIDTLAGKYNISVDGRKNRAL
                     IGKGIIEGKKVILAKPQTYMNLSGESLGGLVDYYKVDEESEFLVVYDDISLDVGQLRIR
                     KKGSAGGHNGMKNIISHLGTEVFPRIKVGVGEKPKKYDLADYVLSRFSKEERAIMEEGC
                     QKAVEAVEMILRGEMEEAMNKFNRKVKPKEA"
     gene            20097..20531
                     /db_xref="GeneID:62697608"
                     /locus_tag="HDCHBGLK_RS17215"
                     /old_locus_tag="HDCHBGLK_03428"
     CDS             20097..20531
                     /codon_start=1
                     /db_xref="GeneID:62697608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605076.1"
                     /locus_tag="HDCHBGLK_RS17215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03428"
                     /product="hypothetical protein"
                     /protein_id="WP_004605076.1"
                     /transl_table=11
                     /translation="MSSKTKIVVLHMKEIIYTAVFAALAIILILLLVFMFLPKNKESKT
                     NEEKYMPGVYTSTVTLNNTALEVEVTVDETHINSIRFSNLDESVTTMFPLIQPTIEDIA
                     EQVYDSQSLDNIQLSEDSPYTSQIIVNAIDEALKKAETTK"
     gene            complement(20565..20828)
                     /db_xref="GeneID:62697609"
                     /locus_tag="HDCHBGLK_RS17220"
                     /old_locus_tag="HDCHBGLK_03429"
     CDS             complement(20565..20828)
                     /codon_start=1
                     /db_xref="GeneID:62697609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440037.1"
                     /locus_tag="HDCHBGLK_RS17220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03429"
                     /product="hypothetical protein"
                     /protein_id="WP_004605075.1"
                     /transl_table=11
                     /translation="MYEYDEECLKTFLKNQAQLFDEPVAETLEEAEAFLEDCMAAVVEN
                     IGEVKEFLEEEGLDVDSMSLEEIEEASEVFPLPGGRYLIVEG"
     gene            complement(20862..21509)
                     /db_xref="GeneID:62697610"
                     /locus_tag="HDCHBGLK_RS17225"
                     /old_locus_tag="HDCHBGLK_03430"
     CDS             complement(20862..21509)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:62697610"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248623.1"
                     /locus_tag="HDCHBGLK_RS17225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HDCHBGLK_03430"
                     /product="HAD family hydrolase"
                     /protein_id="WP_009248623.1"
                     /sec_met_domain="HAD_2 (E-value: 6e-40, bitscore: 135.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTCIFDLDGTLTDTLESLAFSVKETLKEMGLPQITTDECRSFVG
                     NGARYLMERSLEAAGDRELSRIEEGMEVYGRIFDENCTYLVTPYEGITGTLMQMKDKGI
                     KLAVLSNKPHRQTKKVVERIFGEDVFDCVQGQKEGIARKPDPAGIYRLLEEMQAGREEC
                     LYVGDSEVDVETGRNAGVRMVGVSWGFRSREVLRAAGAGTIIDSPEELMQFV"
ORIGIN
        1 cttaccagcg taggatcaaa acggatacca aactttgctt ccttggcttc ttccacgctt
       61 gagataatcg tctccatccc ctcagccgtc tctgatgaaa ggcagcagcc aagtttctct
      121 ttcagataac ggataccttc cacatccggc gttacctcgt cgaacagacc aagataggta
      181 tccacgcttt ctttgccata ccccagttcc tgcagttccc cggccacgcc ttcttttcca
      241 atcttatcca tcttgtcaag gacaatgaag acttcgtcat aatcttcttc tttaaaaccg
      301 ctgtaggcgg ccattgcctt caggatgttc ctgtcattga tgcatacggt aaaattctga
      361 aagttcagtt tccccagcat ggccgtggtg gcgagaatca attcgatctc tgccagtatg
      421 cccggctcgc ctaagatgtc gatatcgcac tgcataaact ggcggaacct gcccttctgc
      481 ggacggtcag ccctccatac atttccaatc tgcatcgcct tgaatggcga tggcagctcg
      541 ctcatatgat ttgcataata tcttgcaagg ggcacggtca agtcgtacct cagtcctccg
      601 tcaaccagat cattttcctc ttttgcctcg tctatcttca atttctcgcc gcgctttaag
      661 atcttgaata tcagtttttc attgtccccg ccctgcttgc tgcagagatt ctcgatatgc
      721 tccacgcaag gggtttccat tgatgagaat ccataagtct tgtaggtttc ttttatgagg
      781 tggatcacgt aatcacgaat ctccatttct tttggaagca tgtccttcat tccggtaacc
      841 ggtttcttct ttaacgccat agccattctc cttttcgttc tatcatttca tcaatccggt
      901 ctatataggc atcgatcgcc tttacattct cgatccgcat cagccgcgtc atctttttct
      961 ttctgctgcc aggcatgggt attatgcctt ttagtacgat cttcgtggat gcgcctgcgc
     1021 cagcggcaac tatggattgc ttttcctcca taataagtat attgtatatt cccgctttgt
     1081 caacctttgc atagccaaca ttttcgaaat tccccgcaat attcttctgg cgatacaaat
     1141 aatatggggc cagtcccatc tgccttgcca ggcgctctgc tgaccgaatc atgcctgaca
     1201 tgacttcatt tgtcttcccg tcctcccgct tcagatcctc catatccatc cttgccgccc
     1261 ttttgatggc aagggaatgc accgtcaggc tgtccggcat aagccggcca atctgcctca
     1321 gggtatcttc catatcatgg agatcctctc ccggaagacc tgcgataata tccatattaa
     1381 tattgtcaaa ccccagttcc ctcgccatgc catatgccgt tatgatctgc tctacgctgt
     1441 gcctgcgccc gatggcatcc aacgtctttt gctgcatgct ctgcggatta atggagatcc
     1501 gggtgacgcc gtgacgtctg atcgcttcta gcttctccct ggtaatgctg tccggccgtc
     1561 cggcctccac cgtatattcc agaagatgct cccttgagaa gttctcgtca atacagcaca
     1621 ggaggcgttc cagctgtccc gccgtaagcg tggtaggcgt gccccctccg atgtagatag
     1681 tattcagttt ctttaaggag gacttctgag cgataaactt caactctttg cacaaggcat
     1741 ccagataatc ctccaccctg ctttcccagt cggctaaggc cccggaacta aaggagcagt
     1801 agctgcacac ggtgggacag aacgggatgc ccacataaag gctgtaccca ttgtcatagt
     1861 ccagccgctc tagcagttcc ttctcccttc cggcaatctc ccatgccagg gaagccttct
     1921 ctttgcttac catatagatc tcgtgaaacc aggagacaaa tgccttctca tccatcccct
     1981 catccagctt cttcatggca atcttcgtag gcctgactcc ggtaagaatc ccccaggaaa
     2041 gagagttccc cgtctcttct tcaagcaggt gatataactt tacgtctata tgccgtttaa
     2101 cttctgcttc ttttcctatc cctgaatatt cggcgggcat atccgccgcg cctgtcgtga
     2161 tcttgcgccc attctccagc agtaccgtta cataattaga ggctttttcc tccacccggc
     2221 acacagcctg tgccgctgga taaaaagcct taatcatatg gtacgtatta taagtatatg
     2281 cctcgctgtt acatataatc cgaatcattc ttataccccg ctacagaaat ggattgtgcg
     2341 tcttctcatg cccaatcgta gtctcgccca tatgccccgg atatacaagg gtctcatccg
     2401 gaaggaccat cagtttctcc ttcacggccc gcaccaggtc ggacatgctt ccgcccggaa
     2461 aatccgtacg tccgatcgaa ttctggaaca gcgtatcccc gctgaataag actccttcgg
     2521 atctgacata atagcagcat cctcccttgg tatggcccgg cgtgtggaac acctcaaagt
     2581 catagccagc cagtttcaga atctgtcctt ccttgatgct cttagctcct tcataagtat
     2641 atcccccggt atagacagaa gactggttta acttcggatc cgtcatagca tccgcgtctt
     2701 cttcatagac atagaccggc acatggaatt cgtccacgaa tccgtcaatt cccataatat
     2761 ggtcaaaatg ggcatgcgtc agtagaagcg cctctatctt aattccttcc gatttgatgt
     2821 ggcttagaag ataggacgga caggcagccg gatcaaccac gaccgcctgt cttgtctctt
     2881 catttaccgc cagataacaa ttagtgctga taattccagt aacaaatctc tctactttca
     2941 tcttttctat cctgtcgtcc tttcaatatc aattacgccc tccacctgac gaagtttctt
     3001 cacaattctt gtcagttctt ccctgccatg cacgataaat cccgtctcaa tggtagcggt
     3061 tccctgcttg ctggtgcgta cattcatgga cttcacatcc acgtctcctt ccgtaaagat
     3121 ctttgacatc tccatcaaaa gcccttgcct gtcatgggca tacatcttga tctcagccag
     3181 atactgcccg ccggccttct ctgccacgtc gctttcccac tccgcatcaa tcaggcgggc
     3241 gcgctcggat tctgtcagat ggatcatgtt cacgcagtcc gtgcgatgga tggaaaggcc
     3301 tctgcctctg gtaacaaagc ccacaatctc atccccgggc accggattgc agcatctgga
     3361 gaaacgtact gccatatcgt caattccttt gaccacgatt ccactcttgg acttggcaat
     3421 atgcacctta ttcttggacg cttctgcaac ccgctccaga accacttcat ccgtgatttc
     3481 ctgcttgtgc tctttgtcat actcttccac aagccggttt accacctggc cttccttaag
     3541 ccctccgtgc ccaatggcag ccagcaccgc ttcccagtcc ttgaatccgt atttcttctg
     3601 caccacgtcc tgatatttcg gattcatgat attgctcaaa ttaatggatt tggctttgca
     3661 ataagaggca atcatctctt ttcccttgat gatattgctt tccttgaatt ctttcttaaa
     3721 ccattgatta atcttgttct ttgcctgggt gctcttgacg attcccagcc agtcccggct
     3781 cgggcctctg gagttctggg aggtcaggat ctcgatcctg tccccgttct gtatcttgta
     3841 ttcaatattc accagcttgc cgtttaccct ggcgcctacc atcttatttc ccactgcgct
     3901 gtggatggcg tatgcgaaat caatcggcgt agagccattg ggcagattct tcacatcgcc
     3961 ttggggcgtg aagcagtata catcctctgc aaacaggtcc agatcgccct tgatcaggct
     4021 taaaaactcc cgattatccg acatgtctct ctgccattcc agtatctggc gcagccagct
     4081 aagcttctct tcttcctgcg cctccacgct cttcttgcca tcattggatt ctttatattt
     4141 ccaatgggcc gcgataccgt actctgccgt cttatgcatt tcctgcgtcc tgatctggat
     4201 ctcaaaaggc tgccccacgg aactcatcag cgtcgtatgc aaggactgat acatattggg
     4261 cttgggcatc gcaatatagt ctttgaagcg tcccggtatc ggcgtataca tctcatggat
     4321 gacacctagc gccgcatagc agtcctttac ggagtccacg ataatccgca ccgcgaacag
     4381 gtcatagacc tgatccaccg tcttatcctg attaaccatc ttcttgtaaa tgctgaagaa
     4441 atgcttgacg cgcccgttca catccgcctt gatgctggcg ttcttcatgt gggtggacac
     4501 ttcatccacg atctgctgga cgaattcttc ccgctccgtc ttccttgcgt taatctgatt
     4561 tacaagatca aagaatactt ccggctgcga atacttaaga gccaggtcat ccagttccgt
     4621 cttaatcttg gatatgccga gccgctgggc aatcggcgca tagatatcca tggtctcttt
     4681 ggctttctcc ttctgcttgg ccggcgtcat aaactccagc gtccgcatgt tgtgaaggcg
     4741 gtcggcaagc ttgatgatga tgacccggat atcctttgcc attgccagga acatcttcct
     4801 taagttctct gcctgtacct ccaacttgtc ggaagaatag gaaagccttc ccagcttggt
     4861 gacgccgtct accagaagcg ccacttcctc gccaaattcc cgtttaatat cctcttcgcc
     4921 tacttccgta tcctcaacaa cgtcatgaag catgccggat acgatcgtct ctttatccat
     4981 ctccaggtca gccagtatga tggctaccca cagaggatgc acgatatacg gctctccgga
     5041 cttgcggcac tgccctccat gggctttttt cgcaagctgg tatgcctttt caatcataga
     5101 aacatccgta gaggggtgat accttcggat gcgggcaatc aacatttcat ataagcgatc
     5161 cggattttca tagtctcccg gcgctttaac ggcatgaccg tcaacgactt ccagtcctgc
     5221 ggaggcatct gccgtcagcg attctggcgc aatacgcccg tcaattgcct catatgtggt
     5281 tttttctgac atattaacgc ctccttcctg ttacatattt ttcttttgtt attatttacc
     5341 aggatatgca attacagact caacgtcata tccttttaat ttatctctgc ccttaagacc
     5401 ctccagctcc atcaaaaata cggtctttac gacttctcct cccaggcctt ccaccagttt
     5461 gatcatagcc tcattggttc cccccgtggc gatcagatca tcaataataa ccaccttctg
     5521 gccaggcttt atcgcgtcac ggtgcatctc caattcggca atgccatact ccagctcata
     5581 tttgattgaa accgtctcac atggaagttt tccctttttc cggattggaa tgaatggctt
     5641 atgaaggtta taggcaatgg gaactccaaa gataaatcct cgggactccg gccctacgac
     5701 cacatcgaac tccacatctt ttaatttttc ctgcataagg tctatggcaa gcatcagccc
     5761 gtcggcatcc tgaagcacgc tggtcacatc cctgaatata atacctgatt ccggaaaatc
     5821 cggaatactc cttacgtatt cttcaatcgg tttcatacac aatctctcca ttctcactaa
     5881 aataccttat aagtatagca ttagtctacg taattttcaa acaattctag acatttctcg
     5941 caccattatt ggtagtgcgt aatcacaatc tgaaccgtac gctttcccat atattcatta
     6001 atgtcgggat aataagtgac ggacagcttc atctggcctc ctcttccttt catcagaaga
     6061 tccacagcct ctttcccata ccgctcttcc agaagggaaa gcatctgctc tgctccccgg
     6121 aagcacacgg cctcccttgc cgtctgactg gaatctttta cctgaagttt cagtacattt
     6181 ctattcttgc caagaatccg cccgcttata agttctacat ttctggcggc aaagaccggc
     6241 tttgtattcc cctttccgaa aggttccaaa agagtcagtt cctccaccag ttcctctgtc
     6301 acgcaggaaa acggcaattc catatctatc actacttttt ctgccatgtc ttttggctcc
     6361 agccggcagt tatcattgag cgtccttcga agttcttcta tgttttcctt ggcaagggac
     6421 attcctgccg ccagcttatg tcctccgaac ttggtgagga gatccctgca gcggctgagt
     6481 tcttcataca tattatatgc ctctatggaa cggcccgagc ctttgatacc ctcttcggca
     6541 tccgtcagta tgatggcagg cctgtaatac cgctcccgga tccggcctgc cacgattccg
     6601 gccaggcttt catggcagcc gggcagatag aggaccagaa cccggtcttt ccctgccttc
     6661 gtcgtgtcca cgatctgtct tgcctgttcc accgcgatat ccgtcatctc tttgcggctg
     6721 tcattcagcg ccttcagatc ccctgccagt atgtccgcct cttttccgga tttcgcccgc
     6781 agcagattaa gcgtccgctt tgccgtatcc agccttccgc tggcattcat acaaggccca
     6841 agtacgaatc cgatatgata ggcgccgatt cgatccttat ccactcccgt gcacgcgatc
     6901 aaagagcgaa gtccgggact atgcgtccgc ttcagcatct gcaggccttc ctttacgaaa
     6961 atcctgtttt cgttttccag ttccatcaca tcgcccactg tagcgatcgc cacattttcg
     7021 atcagatagt ccagatcctc cgcgtcgccg cccgtggcct cccacagggc ttccaccagt
     7081 ttataggcga tggctgcccc gcacaaccct ttaaatggat attcgcaccc cggctgcttt
     7141 ggatcgatta cagcatctgc aggcggaagc agatattttt tcccgtcctc ttcttcctcg
     7201 aagggcacct cgtgatgatc cgtcaccacg atcgtcatcc caaggcgctt tccgcaggca
     7261 atctcctccg ccgccgcaat gccattgtcg caggtaatga tggtatctat tccatcctga
     7321 tacgcccgtt caatcaattc gatgttcagt ccatatccat ccttcatgcg gctgggtata
     7381 tcactgtctg cctcagcccc cagtttctcc aagccctcca gcagtatgta ggtggcattc
     7441 actccgtcaa tatcgtaatc tccgatgacc cggatccgct tctgctcggc aatcttctcc
     7501 cttaagatgt ccactgcctt gtccatatcc ttcatcagca tgccgtcata caggtcggca
     7561 attgtgccat tcaggtactg cgcgattgcc tcatcgccga tcacatccct gttacggatt
     7621 aagcaggcca gcctggggct gatctgaaac cttttcccaa tcccttcaaa atccgcgcct
     7681 tttcttagaa gcacccattg ttccatcctc tatcctccct atgtatcttg aaagtaaaaa
     7741 gcagccacgt catataaata tggctatggc tgcttcatcg tttcactcta ttatttcttt
     7801 ttcttcttgc ttgtctgggc agctggtttc ttgccagtct ttgtcttcat aacataccac
     7861 agggctcctg tgatgcacac ggacgaatat gctccgcata caatgcccac gatcagcggc
     7921 agcgcaaatt cacggatgga gcttactccc attacgtaca gcactgccac cataatgaat
     7981 gtggtcaggg aagtataaat acttctggta agcgtcattg taatactctt atttaccact
     8041 tctgcaagat ccgtggactt ggtctggtaa tgaagttcct cgcggatacg gtcaaatatg
     8101 acgatcgtcg cattaatgga atatcccacg atggtcagca tgcaggcgat aaatgtattc
     8161 cccaccgata ttctcgcaac cgcatagaat gcaagcacga ctaaaacgtc atgtaacaga
     8221 gcggttaccg cgctggttgc aaagcgtata tccttgaatc ggaaccagat atacagaagc
     8281 atgaatatag ttgcgacaat aaccgcgaca accgcgtcct gccgcatctc gttacttact
     8341 gtagaactga tattctctgc agtaatcttg gactcatcca cgccgaaatt atctgccata
     8401 gcctgattca gtttttcacg ctggtcaagt tccagcgtaa ccgtcttgat aatcacctgg
     8461 ttggtacctg ccaccttctg cgtctggaca ttgtgatcgc cggtcacttt ttctactaca
     8521 ggcacgatct ctttgtcaat ctcttcaatc gtgtaatctt cattaaatgt cacattggtt
     8581 gatgttccac cttcaaactc aaggctgtat gcaaatgccc cattcccctt ggatgaattc
     8641 acgcccatga agatcagtcc tgcggcgatg actgccgtag atacggtaaa gaaccatttc
     8701 ttctttccga ggaagttgat ggttttcctc tccttcttag cacgatagta tagtttttct
     8761 ttgcgaagcc ctaccgcata gaacgcatac acgatcatac gggtaataac aagggcggta
     8821 aacatggata cgataatacc gattgccaga gtctgggcaa atccctttac cgttcccgag
     8881 cccttgaacc agaggacagc agccgcgatc aaggtggtga tgttgccatc cacgattgct
     8941 gacatagcct tctggaaacc agtctttaat gcattcttca cgctctttcc gcgggacatc
     9001 tcctcacgca cccgggcgaa gatgatgacg tttgcatcca ccgccatacc gatactcagg
     9061 atgatacccg caatccccgg caaggtaagg gtcacgttaa acgcatttaa tataagaagc
     9121 acaagttccg tataaataac cagcgcaagg cttgaagcaa gtcccggcag ataatataca
     9181 aatatcataa agataaatac aatagcaagt ccgatcgcgc cggccttcag actggtgctg
     9241 attgcctgtt ctcccagctg ggcgcctacc acgttggagc gtagttcttc cagttccagc
     9301 ttcaatccgc cgatacggat ggtagatgcc aggttttccg cctcatcata cgagaaattt
     9361 cctgtgatat atgcctgccc tccggtaatc gccgtctgga ctctcgggct gctgatcgcc
     9421 tctccgtcat agatgatgga aatggtcttt cctacattgg cctctgtggc atccgcgaac
     9481 ttctttgtcc cttccttatt cagactcagt tccacggagt actccttatt gcccatatca
     9541 tcctcgccgg ccttggccgt agcggtcttg acatcggatc cggtaatgac cgtctctcca
     9601 tcttccgtcc tgaactcaag ggagcctggc tttccaagtt catccaggat cgcattggca
     9661 tccgtaacgc ccggaatctc aatgttaatc cggtcgtcgc cttcctggta cacgctcgct
     9721 tctgtgctgt actgttccac acgcttctga agcttataaa tggtgtcgct catctctttt
     9781 tctgtcggat tgtcatcctt aacctgatag gtaatgctga cgccgcctgc cagatccagg
     9841 ccaagcttga tgttcttcat tgcgccggtt ccagtcttgc caaaaccgac cagaaccgta
     9901 aagccaagca gcacgaccag cacgacggtc agaatcaggc tcagtatgcc tttgttcttc
     9961 ttcatggttt acttcctttc tgtctcttct taatcccttt ttattcttct gcaagcgccg
    10021 ctttcatcat aattgcgcat tccacgcgct tacgctgcat ctcgcagcca atatcatatg
    10081 tatcatggat ggtcccgtgg aaattgtaag aattcgccat acatccgccg ctgcaataga
    10141 acttggcaaa acagtccctg cacttatctt tggagtacac gttgcagctg cggaaatcat
    10201 ccgcaatctc cggccttacg attccttcgt ccacatttcc catcaggaat tctacctgtc
    10261 ccacgaactg atggcaagga tagagatccc cccaaggcgt cacggccaga tattccgtcc
    10321 cggagccgca tccggacagc cttttcgcta cacaaggccc accgtctaag tctatcataa
    10381 aatggaaaaa attaaaacct tttccttcct tttcacgttc aatcattatc tttgccagtt
    10441 tatcatattc ttcattaatc accgggatat cttcctcccg gatcgcgtag ggatcgcttt
    10501 cctctcccac caccggctct atggacatct gcttgaaccc cagatccgcg aaatgcagga
    10561 tatcgttgga aaaatccagg ttatcgcggg taaacgttcc ccgcacatag tatcgctcct
    10621 ggttcctgct ctttgccagt ttctggaact tgggaacgat caggtcataa cttccctttc
    10681 cattgcggaa gggacgcata cggtcattga catccttgcg gccgtccagg cttaagacta
    10741 cattgtccat ctccttgttc acgaactcct gcacctcgtc gtttaacaga acgccattgg
    10801 tggtaatcgt aaaccggaaa tgcttgtcgt ggatcttttc ctgctctctt ccataagcca
    10861 ccaggtcttt caccacctgc cagttcatca gcggttctcc tccgaagaaa tccacttcca
    10921 gattatgcct gttgccggaa ttggcaatca ggaaatccag cgctttcttt cccacttcga
    10981 aactcataag ggcacgtctg ccatggtact ccccttcttc cgcgaagcag tatttgcagg
    11041 ccagattgca gtcatgggca atatgaaggc ataatgcctt tactaccgtc tttcgcttct
    11101 tcacttcctc gatcatgcat tcataggcat cctcagcgaa tagccttcct gcttccgtca
    11161 gttcgattac gtccgccaga gcatcttcaa tctcgccctc ttcgtacttc tgtcccaact
    11221 ctttcttaag gcccttaagc acgcatggct cttttagagt cttagcggta tgggcttcat
    11281 tttctttgct taatttcgca atcacgtcat agcacagttc gtccactaca tggaccgcgc
    11341 cgctgtacac atccatgacg atattgtatc cattattctg gtattggtga atcaaggcta
    11401 tatacccctt tcctatatac attatcaggg gccgtcccct gcatccattc ttatgaacaa
    11461 caagaaacag cgacccttaa gaccgctgtc ccgagtattc catataatcc aaaactattt
    11521 cttctgttcg caagtctggt ttcctactgt acaggaagtc ttacatgctg actggcaaga
    11581 tgtctggcat tctccgcatc cgctcttctt taaattgcta ttcatagtct gtgtatttaa
    11641 tgtttttaca tgtttcatgc tctattcctc cttacctttg cttaacttat gttattatac
    11701 catttcatta ttattttcgc aatcatttta tatctcttgt taaaacttta attactcccc
    11761 attctatggc ctttttacgg tagttatgat acactatcgg tggattacag cgtgaacgcc
    11821 aggtactgct atctatagcc atagtctttt aaaaagcaat attgcaaatg tatgtcttat
    11881 aggaggtaat tgtacaatgg caattcaaat tggcatcaac ggattcggcc gcatcggcag
    11941 agtcgcattc cgtatcgcca tcagtcagcc tgaacgcttc cacttgtgtg gaatcaatgt
    12001 ccgtaacgca aatcttgatt atatggtata tatgatccgg tacgatacca tattcggccg
    12061 cttccgcggt gaacttggca cttacgagaa ggggattacc gtaaacggaa agaagatccc
    12121 tgtcttttcc gaaagcgatg cttcctgcat cccatgggaa agctgcggtg ccgaatatat
    12181 tatcgatgca accggcgctt tctgcactac tgcaaaggca atgtcccata tcaaggccgg
    12241 ggcaaaaaag gtcatcatct cggcgccagc caaggatgat gagaccccta cgttcgtcat
    12301 gggcatcaac cacaccaatt acacgccgga tatgaaggtc atatctaacg cttcttgcac
    12361 tacgaactgt ctggcgccca tctgcaaagt cctggaggat aattatggca tcgaatatgg
    12421 cctgatgtcc acaatccacg cggcaaccgc caagcagaaa gtagtcgaca gccggtcttt
    12481 gaaggattgg cgcaccggcc gggcagtttt tggcaatctg attccttcca caaccggagc
    12541 agcgaaagca atatccttgg tcatcccttc tctgaaggat cggatgaacg gaatctctta
    12601 tcgggttcct acctcggacg tctccatcgt cgatctaaac gtagcgctta agagcccctc
    12661 atcctacgag gatatttgca gcaaagtcca cgaggcctcc atgacatatt tgtccggcat
    12721 cctggattat gtagatgacg aagtcgtgtc ctctgatttt atcggagact cccacgcctc
    12781 catcttcgat gcacgggaag ggatacaggt taattcccat ttctttaagc tgatctgctt
    12841 ctatgataat gagtggggct ataccagcca gattttcaga ctcatagaat acatggacca
    12901 gattcatcag caataacaat ttcatatcgt ttctatggca gcgatagata gccgaagtta
    12961 ctgtaatcaa ctttctgtaa gggacatatg cattcgtaat tacgattatg aaccatacaa
    13021 aactatgtca aaagagacag acactttttt ggagtgtctg tctcttttac gatatcattc
    13081 cgccgaccat tccgccgccc gcgcataaga taaatgtggt cacgagattg gctccgtccc
    13141 cgttcagcgt atgatacacc cccagcgtga tcagcagaag cagggcaaaa tacgcgatgc
    13201 caagaccaag gccccatatg aaccgcttca ctttggccat cttgccaatc agaattcctc
    13261 cgatcaaggt cgccatgaca tagattgcta cgatcgccgc cgataccatc ttctcgttca
    13321 gctccatctt atatagcgcc atcgtaagca gcaaaagcaa tatccccgta acaatataag
    13381 aagccagcag cgccttcagc acccacataa cctttgagtc ttttcgaacc tgtctttcca
    13441 tcccatccct ccttgtctat cacaagttat gatggaactt ccaggtttat tccactattg
    13501 ttccatttgc tttcctaaaa atcctgcggc gcaggctgcc atcttctgtt ccagttctcc
    13561 cagtttcttc ttcgcgtcct tggatagcag gatgacgccg cctatcacat ctccttcgca
    13621 gatgatgggg cagatgatct catattcgta ttcttcttcc tggctgtcgg ttatggcaac
    13681 atacttctta tcgccaaggg cggccattat ctggtttctg tctcccagct gcttctccag
    13741 cggcctgctg atatatttat cctgcagatc ctttttgcca gtgcctgcag ctgcaatgat
    13801 ctgatccata tcgcagacgc ataccaggca gcccatcgtc tgagcaaggc tctctgcata
    13861 ctgttttgca aatgcagaga gttctccgat gggagaatac ttctttagta tgatttcccc
    13921 ctctctgtcc gtaaatattt ccagagggtc gccttccctg attctaagag ttcttcttat
    13981 ttcctttgga ataaccaccc ttcccagatc atctatcctt cttacgattc cagtagcttt
    14041 catataagtt ctcctccgtt tacaaatcta tccattttct tgtatggtgg tagtctttgt
    14101 aaaatcaagt tttttatacc tgcggccaaa ataaatgtca tttatatatc ctactttggg
    14161 ctggatattt aaagcacagt tctgtataat aataagaagt atgatattag gagggataaa
    14221 gatgagcaag attgaagaag tccgaggcgc tatggtgaaa gccatgaaag ccggagacaa
    14281 ggaaaccaag gagactctgt ccatgctgct ggccgccctt aagaacaagg ccattgacaa
    14341 gcgggaagac ttgacggaag cagaggaaat ccaggtgatc ttaaaagaaa taaaacagac
    14401 aaaggaaaca ctggatatga cccctgcgga tcgcaccgag attattgacg agtgcaacaa
    14461 gcgcctgtcc gtattggaac agtatgcccc gaagatgatg gacgagtcag agatcaagga
    14521 cgtgattgag gctactttaa aagaaatcgg cctggatgcg ccaaccgcta aagataaagg
    14581 aaggattatg aaagttctga tgccaaaggt aaaaggtatg gccgacggca aattggttaa
    14641 cgaacttcta atgacctata tgggataaaa ttatgaaacg gaaaagcaag cagaagctga
    14701 tttttcctgt ccaaattccc tggatcagat acattcaaga agcttcagga atttatcgag
    14761 acggattggt cctatgaatg gtagcatcgt gcagcagctt ttatactcac tggcaaaggc
    14821 tggagcggag gttgcattgc atcccccgct ccagccttcg ccgtccggta taaaaaagtt
    14881 ccagcactat atccaaaaac cttactgccc ctcgctatcc gtatccacct gatcatccgt
    14941 cttaacatca ttcgcatacg gctcctgatc ttctaccttc atagtaacgc taagatcatt
    15001 aaaatcaact tttttccaaa cactcttgtt gaccttgata tccgccttct taagccactt
    15061 ctcgcaggtc ttggtgtaga ggtccgtctt tctgtcattt acgatagact gcttcttggt
    15121 atccgtagcc tctctgtcaa acaggctggt aacctttcca acatagcatc cattctcagc
    15181 ctcgatcatc tctgtcactt cgccttctcc cagcttatca gccgctgcga tcaggtctgc
    15241 tgacggggtg gatgaatcct tgtcaaatgt tattgtgctt gcttccacgc cttgttctgt
    15301 ggcaaatgcg ccgaagtctt ccgccgtctt ggctgcttcc gcaaaatcct ctgccttgct
    15361 cttgaccttt gccttctctt catcagacag atctacggat ttgccatccg catccttagt
    15421 cgtataaggg aacagtacat actgcatgct cttctgggct gcctcttcat cggatacttc
    15481 cgtatcagcg tcggcctgaa ttgcctccgt catcttctgc tggatcgcca taagcgtcat
    15541 cacgcgcttc accgtcttta acgagccact gattttttct ttgtcttcca gggcgtttgc
    15601 ctcgtcaaac tcttttgcag tactctcaat ggcattcttt tccttgtcag acagagagac
    15661 ctcatagtct cccatatgct cttccagaag aatcatagtc tccagctgct ttagcacgga
    15721 gtcttttacg gattcttcgt aagtctggcc ttccatcgct tcggaattcc acatgtcttc
    15781 tcccagatat cctgcgtaat aggtctcata ctgggcctgt gtatatcttg catagaaatt
    15841 agcaatgtcg gctgttatct ctttgtcgcc taccacggca accacatcgt cgtctttaaa
    15901 cgatccgcag ccggtaaggg aagtcgctgc aagcatccct gccattgcga gtatcaatat
    15961 cttctttttc atgttcttgt ataccctcct tagtaccgtt aacttctcta ttatatcctt
    16021 tttttactgg tcaatcaatc ctttaattcc aattagcaca tttttcacaa ccgccagcac
    16081 atccgtattt ttctcttttt tattgcgact cttcttctca tataagaagc aaggattctc
    16141 tgcatctgcc ttaaaggcca gatccccttt aaactgctcc aggagtccgg gaatcttctg
    16201 gggctggact ttggcctttt catacatgga gaaggtgaat ctttctccct tctgctctac
    16261 tgccgtcacg taggcgctgt gagcaatcgc tttcaggctt gcgacagcca ggagagtctc
    16321 cacctttttg gggatatctc cgaaacggtc aatcagctct tccagcatgt cttccatctc
    16381 ttcttcattt tcaatggccg ccacccgctt atagatatcc agtttctgat actcgttggg
    16441 aatataggaa tccgggatat atgcatccac attcagatcc atcgtagtgt tgaaagtctc
    16501 ttcttccatc tcgcctttta aatgcttcac cgcctcatta agcattttgc agtataggtc
    16561 gtatcccacg gcttccatat ggccatgctg ctcagcgcct agaagattgc cggcccccct
    16621 gatttccaga tcacgcatgg caatcttgaa tcctgatccc aagtctgtga actctcggat
    16681 cgcagccagc cttttttccg caacttcccg gagcagttta tccctccgat aaagtagaaa
    16741 ggcataagcc attcggttag aacgccctac tcttccccgc agctgataga gctgggacag
    16801 gcccagatga tcggcatcat ggataatcat cgtatttaca ttggagatgt ccagcccggt
    16861 ctcaataatc gttgtggata ccagcacgtc tatctctccg ttgataaagt cgtacattat
    16921 cctctccagc tggtgctcct tcatctggcc gtgggcgtat tccaccgtga catccggaac
    16981 cagtttctgg atatggcctg ttatctctgc tatatcctcc actcggttat agacataata
    17041 tacctgcccc tgccttccgg attctctttc gatcgcttct cttaccatct cgtcattata
    17101 ctccatgacg taggtctgga tcggcatcct atcgttggga gcctcttcca gcacgctcat
    17161 atcccggatt ccgatcaggc tcatatgaag cgtccgcgga atcggcgttg ccgtcagagt
    17221 taatacatcc acattttcct tcagtttctt aatcttttcc ttatgctgga cgccgaaacg
    17281 ctgctcctca tcgatgatca gaagtcccag gtccttgaat ttcagatcgt cgcttaagac
    17341 tctgtgggtg ccaatcacaa tatccaccaa gcctttcttg gtatcctcga tggtcttctt
    17401 ctgctgagcc ggagtccgga atctgcacat cagatccacc cggaccggaa agtccttcat
    17461 cctctgggca aatgtattgt aatgctgctg ggccaggatc gtggtgggta ccagatagac
    17521 gacctgctta tcctcctgca ctgccttgaa ggccgcccgg atggcaatct ccgtctttcc
    17581 atatcctaca tccccgcaga ttaggcggtc cataatctta tgactcatca tatcctgctt
    17641 gacggcctcg atggcaagca gctggtcttc tgtctcttca aaggggaaca tttcctcaaa
    17701 ttccctctgc cagactgtat cctctccata tacgtagcct tcctgctcct gtcttgcggc
    17761 gtacagcctg accagatctt tggcaatctc ccttactgcc cctcggactc tggtcttggt
    17821 cttcgtccat tcctgcgtgc ccagacggtt taatttgggt ttcttggcat ccgcgcttgc
    17881 atatttctga atcaggtcca gctgggtagc cgggatatat agattgcctc cctgggcata
    17941 ggatatcttc atatagtcct tggatatctt atctacctcg atcttctcga tgccctggta
    18001 tatccccagg ccatggttct catgaaccac ataatctcca accttcagtt ccgcaaaact
    18061 ctgtatcttt cgcccttcat aggtcttacg cttcttcttt ttcttcgtct ttccgaagat
    18121 atccgtctcc gagatcaccg taaacttcag catcggatac tcatagccat atgccacatg
    18181 cccataagcc gtcataatct cgcccggcgc cacttcccgg tccaactcat cgctataata
    18241 actgctcaga ttataatccc ttagatcctc tgccagacgc ttcgccctgg tcctggatcc
    18301 ggatagaaga accaccctgt atccattcct tttcagacgc ttcaaatcct gcgtcagcat
    18361 gtcaaaactg ctgttataag gattgacgct tttcgtctga aggctgaagg aggcctttgt
    18421 ctccagtccc ctgcatttca tatccagggc aaaaaagccg acgctgctat aagcgttcat
    18481 ttttcttaag atctcttccg tatgatacag ctgcacttcc gcgtccgtga cttcgtatcc
    18541 gctttccacc ctctttttct gggcttccag aaactcttct tccaccccct gccctttttc
    18601 gatcaggcgg accggctcat ctaagaacag gatcgtctct tccattggaa aataatccaa
    18661 aaaggaaacc cccttctctc cttcctgcgg aaattccacc gccggacaga tcgacacctc
    18721 ttccagattc tcgatagatc tctgggtctc cacatcaaaa gtacggatcg aatccacttc
    18781 atctccccac agttctatcc ggatcggaag ttcttctgta aggggataga catccagtat
    18841 gccgcccctt accgcaaact ggccgggagc ctcaatctgc acttccctgt catagccaag
    18901 cgccaccagc ctggactgca tctcttccag atgaaaggta ctgtcatttg caatccgcac
    18961 cgtcttttca acaattacat ccttgggcag aagggtgtcc atgaacgcat cgaaactggt
    19021 aatcaccgta gccttttcgc ctgccgccac cgcctggatc acttccatcc tctgacttac
    19081 cagttccttg ctccttaagt ctgcctggta aaagagcagg tctttggccg gatacagatg
    19141 ggtattcgga tccaggaatc gatactcttc atagacttgt tttgccttgg attcacttga
    19201 acaagcgatg actctgtaat tacagccatc gctcaacgca tacatcatat gggttttctg
    19261 ggaattcacg catccgcaga ctcgtatcat ccccgcgcct tttcttatgt ccctgctaat
    19321 ctcttcaaat tctgctaatt cattcagtgg cctgattaag gcttgcatat cttaggcctc
    19381 cttaggcttt acttttctgt taaacttatt catggcctct tccatctctc ctctgaggat
    19441 catctccacc gcttccacag ccttctggca tccttcctcc atgatggcgc gctcttcttt
    19501 cgagaaacga ctgaggacat agtctgccag atcatatttc ttcggctttt ctcccacacc
    19561 gaccttgatt cttggaaata cttccgtccc aagatggctg ataatattct tcattccatt
    19621 atggccgccg gcgcttcctt ttttacggat tctaagttgt ccgacatcca gactgatatc
    19681 atcgtagaca accaaaaact cgctttcttc atcaacctta taataatcca ccaggccgcc
    19741 caggctctcg ccgctaagat tcatgtaggt ctgcggctta gccagaatta ctttcttccc
    19801 ctctatgatt ccttttccga tcagggctct attcttcctg ccgtctacag atatattata
    19861 tttgccggca agcgtatcta tgacatcgaa gccggcatta tgccgcgtgc cttcgtattg
    19921 taatgtagga tttccaagtc ccgctataat aaacattctt ctcacctcag tttctattcc
    19981 tttccattct acaagaagcc gtcctatttg tcacgtaaaa gaaatgaata atttgctgtc
    20041 ttttttcata cattatagaa agtaataaat atcatacaca atacaggagg gtctcaatga
    20101 gttccaaaac aaaaatcgtc gtattgcata tgaaggagat tatttacacg gcagtcttcg
    20161 ccgcactggc aatcatactt atcctgctgc tggtattcat gttccttcca aaaaataaag
    20221 agtccaaaac caacgaggag aaatacatgc caggagtata cacatccacc gtcacgctca
    20281 acaacaccgc ccttgaggtg gaagtgaccg tggatgagac acatatcaat tccatccgct
    20341 tctccaacct ggatgagtcg gtaactacca tgttccctct gatccagccg actatcgagg
    20401 acattgctga acaagtatat gactcccagt ctctggataa cattcagttg tccgaggata
    20461 gcccctatac ttcccagatt attgtaaatg ccattgacga ggcattgaaa aaagccgaaa
    20521 ctaccaagta gttgcggctt tttatatgtt cttcataatg attcctatcc ttctacgatc
    20581 agatatcttc cacccggaag cgggaatact tctgatgctt cctcaatctc ttcaagagac
    20641 atgctgtcta catccagacc ttcctcttct agaaactcct tcacttcgcc gatgttctcc
    20701 acgaccgctg ccatgcaatc ctccaggaat gcttccgcct cttccagcgt ctccgcaacc
    20761 ggctcatcaa acaactgcgc ctggtttttc aagaacgtct tcaggcactc ctcatcgtac
    20821 tcatacattt ctctaacctc ccttaatttc ttttccttgc ttcatacgaa ctgcatcagt
    20881 tcttccggac tgtctatgat agttcccgcg cctgcagccc tcaatacttc tcttgaccgg
    20941 aacccccagg atacgcccac catgcgaacc cctgcgtttc tgcctgtctc cacgtccact
    21001 tcggaatccc ccacatacag gcattcttcc ctgcccgcct gcatctcctc cagcagtcta
    21061 tagattccgg caggatctgg ctttctcgca attccttcct tctggccctg cacgcagtca
    21121 aacacatcct cgccaaagat acgctctacc actttcttcg tctgccgatg gggcttgttg
    21181 gagagaaccg ccagctttat tcccttgtct ttcatctgca taagcgttcc ggtgatgcct
    21241 tcatagggtg ttacaagata tgtacagttt tcatcaaata tacgtccgta tacctccatt
    21301 ccttcctcaa ttcttgaaag ttccctatct ccagctgcct ccagggaacg ttccatcaga
    21361 tatctcgcgc cgttgcctac aaaactgcgg cactcatcgg tagtgatctg aggaagtccc
    21421 atctctttta acgtctcttt caccgaaaaa gccaaggatt ccagtgtatc ggtcaaagtt
    21481 ccgtcaagat caaatataca agttttcata atacatatat cctccatgca ataatcatag
    21541 tatgactata ggaaaaattc aaggggaatt tcgtaatcca ttccccttaa gtgagatatt
    21601 ggcgcatcag ccgcagcgca ttcttttcca ggcggcttac ctgagcctgg gaaatctt
//
