LOCUS       NZ_SHOB01000009         9422 bp    DNA     linear   CON 23-JUN-2024
DEFINITION  Bacillus subtilis strain A1 NODE_9_length_9422_cov_295.888004, whole
            genome shotgun sequence.
ACCESSION   NZ_SHOB01000009
VERSION     NZ_SHOB01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Babalola,O., Ayangbenro,A.S. and Olanrewaju,O.S.
  TITLE     Draft genome sequence of three rhizospheric plant growth promoting
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   Babalola,O., Ayangbenro,A.S. and Olanrewaju,O.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2019) Biological Sciences, North-West University,
            South Africa, Corner Albert Luthuli and University Drive, Mafikeng,
            North West Private Bag X2046, South Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. DECEMBER-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 703.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004294565.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 03:44:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,243
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 103
            rRNAs                             :: 8, 6, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 6, 3 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 75 of 109
            Pseudo Genes (incomplete)         :: 50 of 109
            Pseudo Genes (internal stop)      :: 23 of 109
            Pseudo Genes (multiple problems)  :: 33 of 109
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:31:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9422
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SHOB01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9422
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:7830](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..7830)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..3363)
                     /gene="srfAB"
                     /locus_tag="ES060_RS21665"
                     /pseudo=""
     CDS             complement(<1..3363)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (12-415). E-value: 1.3e-115.
                     Score: 378.2. Matches aSDomain:
                     nrpspksdomains_ES060_RS21665_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (521-589). E-value: 8.7e-26. Score:
                     81.9. Matches aSDomain: nrpspksdomains_ES060_RS21665_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (605-892). E-value:
                     5e-106. Score: 346.0. Matches aSDomain:
                     nrpspksdomains_ES060_RS21665_Condensation_LCL.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="ES060_RS21665"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 1.5e-126, bitscore:
                     410.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.1e-18, bitscore:
                     53.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 5.7e-100, bitscore:
                     322.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQITEATFAALFEKQAQQTPDHSAVKAGGNLLTYRELDEQANQL
                     AHHLRAQGAGNEDIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSG
                     AKFAVVNERNMTAIGQYEGTIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKP
                     KGVQIEHRNLTNYVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQK
                     ETYTAPDEIAHYIKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPT
                     DVLAFRKMYGHTEFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLN
                     EALKLVPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSD
                     GTLAFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTSDQ
                     AIEKAELRKQLSLTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNAAQSGGKALAAP
                     ETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELEKSVPLKALFE
                     QPTVRQLAAYMEASAVSGGHQVLKPADKQDMYPLSSAQKRMYVLNQLDRQTISYNMPSV
                     LLMEGELDISRLRDSLNQLVNRHESLRTSFMEANGEPVQRIIEEAEVDLHLFEAKEDEA
                     DQKIKEFIRPFDLNDAPLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYK
                     GEQLPEPALHYKDFAVWQHEAEQKERMKEHEAYWMSVLSGELPELDLPLDYARPPVQSF
                     KGNTIRFRTGSETAKAVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRT
                     NADVQDMPGMFVNTLALRMEAKEQQTFAELLEQAKQTNLSALEHQEYPFEDLVNQLDLP
                     RDMSRNPLFNVMVTTENPDKEQLTLQNLSISPYEAHQGTSKFDLTLGGFTDENGIGLQL
                     EYATDLFAKETAEKWSEYVLRLLKAVADNPNQPLSSLSLVTETEKQALLEAWKGKSLPV
                     PTDKTVHQLFEETVQRHKDRPAVTYNGQSWMYGELNAKANRLARILMDCGISPDDRVGV
                     LTKPSLEMSAAVLG"
     source          1..9422
                     /collection_date="2015-09-20"
                     /db_xref="taxon:1423"
                     /geo_loc_name="South Africa: Mafikeng"
                     /host="Soil"
                     /isolation_source="Maize rhizosphere"
                     /lat_lon="25.8245 S 25.6066 E"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis"
                     /strain="A1"
                     /submitter_seqid="NODE_9_length_9422_cov_295.888004"
     region          1..9422
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9422
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(535..648)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0009"
                     /evalue="6.40E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="ES060_RS21665"
                     /protein_end="943"
                     /protein_start="905"
                     /score="57.1"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
     aSDomain        complement(688..1548)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21665_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.00E-106"
                     /label="ES060_RS21665_Condensation_LCL.1"
                     /locus_tag="ES060_RS21665"
                     /protein_end="892"
                     /protein_start="605"
                     /score="346.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="DMYPLSSAQKRMYVLNQLDRQTISYNMPSVLLMEGELDISRLRDS
                     LNQLVNRHESLRTSFMEANGEPVQRIIEEAEVDLHLFEAKEDEADQKIKEFIRPFDLND
                     APLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFA
                     VWQHEAEQKERMKEHEAYWMSVLSGELPELDLPLDYARPPVQSFKGNTIRFRTGSETAK
                     AVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTL
                     ALRMEA"
     CDS_motif       complement(697..786)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0008"
                     /evalue="1.20E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="ES060_RS21665"
                     /protein_end="889"
                     /protein_start="859"
                     /score="46.6"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADVQDMPGMFVNTLALR"
     CDS_motif       complement(1045..1077)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0007"
                     /evalue="1.40E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="ES060_RS21665"
                     /protein_end="773"
                     /protein_start="762"
                     /score="14.5"
                     /tool="antismash"
                     /translation="HYKDFAVWQHE"
     CDS_motif       complement(1108..1173)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0006"
                     /evalue="2.00E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="ES060_RS21665"
                     /protein_end="752"
                     /protein_start="730"
                     /score="29.9"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       complement(1369..1479)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0005"
                     /evalue="7.00E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="ES060_RS21665"
                     /protein_end="665"
                     /protein_start="628"
                     /score="53.8"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISRLRDSLNQLVNRHESLRTSF"
     aSDomain        complement(1597..1800)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21665_PCP.1"
                     /evalue="8.70E-26"
                     /label="ES060_RS21665_PCP.1"
                     /locus_tag="ES060_RS21665"
                     /protein_end="589"
                     /protein_start="521"
                     /score="81.9"
                     /tool="antismash"
                     /translation="EESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELE
                     KSVPLKALFEQPTVRQLAAYMEA"
     aSModule        complement(1597..4602)
                     /complete
                     /domains="nrpspksdomains_ES060_RS21670_Condensation_LCL.1"
                     /domains="nrpspksdomains_ES060_RS21665_AMP-binding.1"
                     /domains="nrpspksdomains_ES060_RS21665_PCP.1"
                     /locus_tags="ES060_RS21665"
                     /locus_tags="ES060_RS21670"
                     /monomer_pairings="Asp -> Asp"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2089..2154)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0004"
                     /evalue="3.80E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ES060_RS21665"
                     /protein_end="425"
                     /protein_start="403"
                     /score="38.1"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        complement(2119..3327)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21665_AMP-binding.1"
                     /evalue="1.30E-115"
                     /label="ES060_RS21665_AMP-binding.1"
                     /locus_tag="ES060_RS21665"
                     /protein_end="415"
                     /protein_start="12"
                     /score="378.2"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQAQQTPDHSAVKAGGNLLTYRELDEQANQLAHHLRAQGAGNE
                     DIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSGAKFAVVNERNMT
                     AIGQYEGTIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQKETYTAPDEIAHY
                     IKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPTDVLAFRKMYGHT
                     EFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLNEALKLVPPGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSDGTLAFIGRADDQ
                     VKIR"
     CDS_motif       complement(2236..2325)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0003"
                     /evalue="4.90E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="ES060_RS21665"
                     /protein_end="376"
                     /protein_start="346"
                     /score="50.8"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       complement(2854..2913)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0002"
                     /evalue="1.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="ES060_RS21665"
                     /protein_end="170"
                     /protein_start="150"
                     /score="33.3"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       complement(3100..3141)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21665_0001"
                     /evalue="3.90E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="ES060_RS21665"
                     /protein_end="88"
                     /protein_start="74"
                     /score="16.9"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     gene            complement(<3436..>4608)
                     /locus_tag="ES060_RS21670"
                     /pseudo=""
     CDS             complement(<3436..4608)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     6.2e-95. Score: 309.6. Matches aSDomain:
                     nrpspksdomains_ES060_RS21670_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="ES060_RS21670"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="condensation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 2.2e-86, bitscore:
                     278.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMID
                     GEVVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDV
                     FKGELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFKDETIRRWSRHFVNIIKAAAAN"
     CDS_motif       complement(3544..3591)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21670_0006"
                     /evalue="1.00E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="ES060_RS21670"
                     /protein_end="355"
                     /protein_start="339"
                     /score="-0.4"
                     /tool="antismash"
                     /translation="HHVAKFDLSLEAVERE"
     CDS_motif       complement(3655..3768)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21670_0005"
                     /evalue="7.60E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="ES060_RS21670"
                     /protein_end="318"
                     /protein_start="280"
                     /score="53.6"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        complement(3817..4602)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21670_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="6.20E-95"
                     /label="ES060_RS21670_Condensation_LCL.1"
                     /locus_tag="ES060_RS21670"
                     /protein_end="264"
                     /protein_start="2"
                     /score="309.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMIDGE
                     VVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLLID
                     MHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDVFK
                     GELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     CDS_motif       complement(3823..3912)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21670_0004"
                     /evalue="3.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="ES060_RS21670"
                     /protein_end="262"
                     /protein_start="232"
                     /score="51.7"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       complement(4159..4194)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21670_0003"
                     /evalue="4.10E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="ES060_RS21670"
                     /protein_end="150"
                     /protein_start="138"
                     /score="13.1"
                     /tool="antismash"
                     /translation="HYKDYAVWHKEQ"
     CDS_motif       complement(4225..4290)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21670_0002"
                     /evalue="2.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="ES060_RS21670"
                     /protein_end="128"
                     /protein_start="106"
                     /score="29.5"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       complement(4486..4599)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21670_0001"
                     /evalue="5.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="ES060_RS21670"
                     /protein_end="41"
                     /protein_start="3"
                     /score="57.4"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSF"
     gene            complement(4554..4694)
                     /gene="comS"
                     /locus_tag="ES060_RS21675"
     CDS             complement(4554..4694)
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="ES060_RS21675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_010886404.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECISSISLDKQTQATTSPLYFCWRE
                     K"
     aSDomain        complement(<4738..4920)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21680_PCP.1"
                     /evalue="8.40E-20"
                     /label="ES060_RS21680_PCP.1"
                     /locus_tag="ES060_RS21680"
                     /protein_end="1031"
                     /protein_start="970"
                     /score="62.8"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVRAGVTDNFFMIGGHSLKAMMMTAKIQEHFHK
                     EVPIKVLFEKPTIQEL"
     aSModule        complement(<4738..7812)
                     /complete
                     /domains="nrpspksdomains_ES060_RS21680_Condensation_DCL.1"
                     /domains="nrpspksdomains_ES060_RS21680_AMP-binding.1"
                     /domains="nrpspksdomains_ES060_RS21680_PCP.1"
                     /locus_tags="ES060_RS21680"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     gene            complement(<4738..7830)
                     /gene="srfAB"
                     /locus_tag="ES060_RS21680"
                     /pseudo=""
     CDS             complement(<4738..7830)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.4e-14.
                     Score: 45.0. Matches aSDomain:
                     nrpspksdomains_ES060_RS21680_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-305). E-value:
                     2.6e-115. Score: 376.6. Matches aSDomain:
                     nrpspksdomains_ES060_RS21680_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     1.2e-111. Score: 365.1. Matches aSDomain:
                     nrpspksdomains_ES060_RS21680_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1031). E-value: 8.4e-20. Score:
                     62.8. Matches aSDomain: nrpspksdomains_ES060_RS21680_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="ES060_RS21680"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 3.9e-92, bitscore:
                     296.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 9.3e-117, bitscore:
                     378.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.6e-15, bitscore:
                     43.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFD
                     LEIFEKSVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHI
                     ERYKQDVQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNY
                     EALRAGRTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDE
                     YVNKEYSFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGR
                     PSEINGIETMAGLFINTIPVRVKVERDAAFADIFTAVQQHAVEAERYDYVPLYEIQKRS
                     ALDGNLLNHLVAFENYPLDQELENGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFDSAFIERTAEHLTRMMEAAVDQPAAFVREYGLVGDEEQRQIVEVFNSTK
                     AELPEGMAVHQVFEEQAKRTPASTAVVYEGTELTYRELNAAANRLARKLVEQGLQKGET
                     AAIMNDRSVETVVGMLAVLKAGAAYVPLDPALPEDRLRFMAEDSSVRMVLIGNSYTGQA
                     HQLQVPILTLDIGFEEREAADNLSLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILRLV
                     KNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLRE
                     QRISTMWLTSPLFNQLAAKGAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYG
                     PTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYIMDEQQCLQPIGAPGELCVGGIGVAR
                     GYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELG
                     EIETKLNMAEHVTEAAVIIRKNKADENEICAYFTADREVTVSELRKTLSQSLPDYMVPA
                     HLIQMDSLPLTPNGKINKKELPAPQSEAVQPEYAAPETESEKKLAEIWEGILGVRAGVT
                     DNFFMIGGHSLKAMMMTAKIQEHFHKEVPIKVLFEKPTIQEL"
     CDS_motif       complement(5209..5274)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0010"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ES060_RS21680"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        complement(5239..6420)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21680_AMP-binding.1"
                     /evalue="1.20E-111"
                     /label="ES060_RS21680_AMP-binding.1"
                     /locus_tag="ES060_RS21680"
                     /protein_end="864"
                     /protein_start="470"
                     /score="365.1"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAKRTPASTAVVYEGTELTYRELNAAANRLARKLVEQGLQKG
                     ETAAIMNDRSVETVVGMLAVLKAGAAYVPLDPALPEDRLRFMAEDSSVRMVLIGNSYTG
                     QAHQLQVPILTLDIGFEEREAADNLSLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFL
                     REQRISTMWLTSPLFNQLAAKGAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYIMDEQQCLQPIGAPGELCVGGIGV
                     ARGYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       complement(5356..5445)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0009"
                     /evalue="2.80E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="ES060_RS21680"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.4"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGIGVARGYVNLPELTEKQF"
     CDS_motif       complement(5947..6006)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0008"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ES060_RS21680"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       complement(6193..6234)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0007"
                     /evalue="1.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="ES060_RS21680"
                     /protein_end="546"
                     /protein_start="532"
                     /score="21.1"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       complement(6760..6876)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0006"
                     /evalue="1.70E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="ES060_RS21680"
                     /protein_end="357"
                     /protein_start="318"
                     /score="55.8"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYP"
     aSDomain        complement(6916..7812)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21680_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="2.60E-115"
                     /label="ES060_RS21680_Condensation_DCL.1"
                     /locus_tag="ES060_RS21680"
                     /protein_end="305"
                     /protein_start="6"
                     /score="376.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFDLEIFEK
                     SVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHIERYKQD
                     VQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAG
                     RTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEY
                     SFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGRPSEING
                     IETMAGLFINTIPVRVKV"
     CDS_motif       complement(6925..7020)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0005"
                     /evalue="7.50E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="ES060_RS21680"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       complement(7258..7296)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0004"
                     /evalue="1.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="ES060_RS21680"
                     /protein_end="191"
                     /protein_start="178"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       complement(7342..7407)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0003"
                     /evalue="1.20E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="ES060_RS21680"
                     /protein_end="163"
                     /protein_start="141"
                     /score="37.0"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       complement(7624..7740)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0002"
                     /evalue="2.80E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="ES060_RS21680"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.3"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     aSDomain        complement(7744..7830)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21680_NRPS-COM_Nterm.1"
                     /evalue="1.40E-14"
                     /label="ES060_RS21680_NRPS-COM_Nterm.1"
                     /locus_tag="ES060_RS21680"
                     /protein_end="29"
                     /protein_start="0"
                     /score="45.0"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHS"
     CDS_motif       complement(7765..7803)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21680_0001"
                     /evalue="2.40E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="ES060_RS21680"
                     /protein_end="22"
                     /protein_start="9"
                     /score="16.9"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     CDS             complement(7843..>9418)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (1-49). E-value: 4.6e-18. Score:
                     57.2. Matches aSDomain: nrpspksdomains_ES060_RS21210_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (59-362). E-value:
                     7.5e-179. Score: 588.0. Matches aSDomain:
                     nrpspksdomains_ES060_RS21210_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (367-521). E-value: 5e-56.
                     Score: 180.8. Matches aSDomain:
                     nrpspksdomains_ES060_RS21210_TIGR01720.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="ES060_RS21210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_21210"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_250620090.1"
                     /sec_met_domain="PP-binding (E-value: 1.2e-14, bitscore:
                     43.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.4e-98, bitscore:
                     317.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TDNFFSLGGDSIKGIQMASRLNQHGWKLEMKDLFQHPTIEELTQY
                     VERAEGKQADQGPVEGEVILTPIQRWFFEKNFTNKHHWNQSVMLYAAKGFDPERVEKTL
                     QALIEHHDALRMVYREGQEDVVQYNRGLEASSAQLEIIQIEGQAADYEDRIEREAERLQ
                     SSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPV
                     LPQKTHSFAEYAERLQDFANSRAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKT
                     IEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVSVHLEGHGREEII
                     EDLTISRTVGWFTSMYPMVLDMKHADDLGYQLKQMKEDIRHVPNKGVGYGILRYLTAPE
                     HKEDVAFSIQPDVSFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIEN
                     GKLTMSLAYHSLEFHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDE
                     LDKLMEIF"
     gene            complement(7843..>9422)
                     /locus_tag="ES060_RS21210"
                     /old_locus_tag="ES060_21210"
     aSDomain        complement(7855..8316)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21210_TIGR01720.1"
                     /evalue="5.00E-56"
                     /label="ES060_RS21210_TIGR01720.1"
                     /locus_tag="ES060_RS21210"
                     /protein_end="521"
                     /protein_start="367"
                     /score="180.8"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLE
                     FHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     aSModule        complement(7855..9414)
                     /domains="nrpspksdomains_ES060_RS21210_PCP.1"
                     /domains="nrpspksdomains_ES060_RS21210_Epimerization.1"
                     /domains="nrpspksdomains_ES060_RS21210_TIGR01720.1"
                     /incomplete
                     /locus_tags="ES060_RS21210"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(8233..8292)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21210_0006"
                     /evalue="8.90E-10"
                     /label="NRPS-E6"
                     /locus_tag="ES060_RS21210"
                     /protein_end="395"
                     /protein_start="375"
                     /score="30.7"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        complement(8332..9240)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21210_Epimerization.1"
                     /evalue="7.50E-179"
                     /label="ES060_RS21210_Epimerization.1"
                     /locus_tag="ES060_RS21210"
                     /protein_end="362"
                     /protein_start="59"
                     /score="588.0"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVILTPIQRWFFEKNFTNKHHWNQSVMLYAAKGFDPERVEKTL
                     QALIEHHDALRMVYREGQEDVVQYNRGLEASSAQLEIIQIEGQAADYEDRIEREAERLQ
                     SSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPV
                     LPQKTHSFAEYAERLQDFANSRAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKT
                     IEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVSVHLEGHGREEII
                     EDLTISRTVGWFTSMYPMVLDM"
     CDS_motif       complement(8338..8385)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21210_0005"
                     /evalue="9.20E-09"
                     /label="NRPS-E5"
                     /locus_tag="ES060_RS21210"
                     /protein_end="360"
                     /protein_start="344"
                     /score="27.3"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     CDS_motif       complement(8794..8859)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21210_0004"
                     /evalue="1.70E-12"
                     /label="NRPS-E2"
                     /locus_tag="ES060_RS21210"
                     /protein_end="208"
                     /protein_start="186"
                     /score="39.4"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       complement(9061..9135)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21210_0003"
                     /evalue="1.90E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="ES060_RS21210"
                     /protein_end="119"
                     /protein_start="94"
                     /score="20.3"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       complement(9196..9225)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21210_0002"
                     /evalue="3.20E-05"
                     /label="NRPS-E1"
                     /locus_tag="ES060_RS21210"
                     /protein_end="74"
                     /protein_start="64"
                     /score="16.5"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     aSDomain        complement(9271..9414)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ES060_RS21210_PCP.1"
                     /evalue="4.60E-18"
                     /label="ES060_RS21210_PCP.1"
                     /locus_tag="ES060_RS21210"
                     /protein_end="49"
                     /protein_start="1"
                     /score="57.2"
                     /tool="antismash"
                     /translation="DNFFSLGGDSIKGIQMASRLNQHGWKLEMKDLFQHPTIEELTQYV
                     ERA"
     CDS_motif       complement(9382..9411)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ES060_RS21210_0001"
                     /evalue="5.60E-03"
                     /label="PCP_mC"
                     /locus_tag="ES060_RS21210"
                     /protein_end="12"
                     /protein_start="2"
                     /score="10.1"
                     /tool="antismash"
                     /translation="NFFSLGGDSI"
ORIGIN
        1 gccgagcacc gcggcggaca tttcaagcga cggcttcgtg agaacgccga cgcggtcatc
       61 cgggctgatg ccgcagtcca tcagaatccg cgcgagacgg tttgccttcg cattcagctc
      121 gccgtacatc caagactggc cgttgtatgt gacagccggg cggtctttgt ggcgctggac
      181 agtctcttcg aatagctgat gaaccgtttt gtctgtcggc acaggcagcg atttgccctt
      241 ccatgcctca agaagcgctt gcttttctgt ctcggtgacc agtgacaggc tggaaagcgg
      301 ctggttcggg ttatccgcaa cagcctttag tagccgcaga acgtattcgc tccatttttc
      361 agctgtttct tttgcgaaca gatctgtcgc atattcgagc tgcaagccaa tgccattttc
      421 atcagtaaat ccgcccagtg tcagatcaaa cttagaagtt ccctgatgag cctcataagg
      481 tgaaatgctc agattttgca atgtaagctg ttctttatca gggttttctg tcgtcaccat
      541 cacattaaac aatgggtttc ggctcatatc ccgagggaga tcaagctgat taaccagatc
      601 ttcaaacgga tactcctgat gctcaagcgc tgacaggttc gtctgctttg cttgctccaa
      661 aagctccgca aatgtttgct gttctttcgc ttccattctc agggcaagtg tattgacgaa
      721 cattcccggc atgtcctgaa catcagcatt cgtccggccg gcagtaacgg agccgatgac
      781 gatatcccgc tgtccggaaa ttttgcttaa aaagacgtgg aaaacagcat ggagcaccat
      841 gtgcaaggtc gttccggttt ccgcaagcag tttttctacc gcctttgccg tctcacttcc
      901 cgtacggaaa cggatcgtat ttcctttaaa gctttgcaca ggcggacggg cgtaatcgag
      961 cgggagatca agctctggca gctcgcctga aagaactgac atccagtacg cctcatgctc
     1021 cttcatccgt tctttttgct cagcttcatg ttgccaaacg gcgaaatctt tataatgaag
     1081 cgccggctcc ggaagctgct ctcctttgta aagcagcgcc aattctttta caaagatgcc
     1141 tcttgagacg ccgtctgcga tgatatgatg catatctaaa agcagcaaat gttttttcgc
     1201 ttctattcga agcaaagctg cgcgaatgag cggtgcgtcg tttaagtcga atggccggat
     1261 aaattcctta atcttttggt ccgcttcgtc ttctttggct tcaaacagat gaagatcaac
     1321 ctccgcctcc tcaatgatgc gctgaacagg ctcaccatta gcttccataa atgacgtccg
     1381 caatgattcg tgacggttca caagctgatt gagtgagtcg cgcaggcgcg aaatatcaag
     1441 ctctccttcc attagaagaa cagatggcat gttgtagctt atcgtctggc ggtcaagctg
     1501 attgagcacg tacatccgtt tctgtgcgga tgacaatgga tacatatcct gcttgtcagc
     1561 cggtttgagg acttgatggc cgcctgaaac ggcagacgcc tccatataag ccgccagctg
     1621 gcgaacagtt ggctgctcga acagtgcttt aagcggtacg cttttctcca attccgcctg
     1681 aatgttggca atcagcatca tcccttttaa tgaatggccg cctaaatcga aaaaattgtc
     1741 gtcaatgccg atggcttcta tgccaagcgt tttctgccaa atgcggcata agctttcttc
     1801 aagcgctgtc tccggtgcgg ccaatgcctt gcctccggat tgtgccgcgt tcggcttcgg
     1861 caaagcgttt ctgtcggttt ttccgtttgc cgtcagcgga atcgcgtcaa cctgcacaaa
     1921 gaaagcagga atcatatgag acggcagtgt tagggaaagc tgcttccgga gctctgcttt
     1981 ttcaatagct tgatccgacg tataatacgc gcaaagctct tgaagaccgc cctcggaaac
     2041 cgctaataca accgcttctt gaattccgct gaggctgagc atgaccgttt caatttcttt
     2101 cggctcaatg cggtagccgc ggattttcac ctgatcatca gcccggccga taaatgcaag
     2161 cgtaccgtca gaaagtcttc gtacgacatc tccggttttg tacatgaggc ttcccggcga
     2221 atatggattt tctacaaatc tctcggctgt cagctgaggc ctgttgagat accctctcgc
     2281 aagcccctgt cccgtgatat agagctgtcc gctcgctccc ggcggcacaa gcttcaatgc
     2341 ttcatttaag acaagcgcgc cggcattcgc aatcgggcgt ccgattgtcg ggcgtttcgc
     2401 aaatgcatcc ggctcagaca gatcaacccg cccggcgatg gcgccgatcg ttgcttctgt
     2461 cgggccgtaa tgattgataa attcggtatg tccatacatc ttacggaagg cgagaacatc
     2521 agtcgggatg attttttctc ctcccaagac gatcaagcgc aaggattcaa agttcgcatc
     2581 ttttgcaaaa ctggcggtgt tcactattgt atggaagaga gacggtgtca gtttgatata
     2641 agtgatccca tgctccttga tatagtgcgc tatctcatcc ggcgccgtat aggtttcctt
     2701 ctggacgata tggagctcgc ccccgcccag aagtacaggg aacatgctcg tatagccaag
     2761 gtcaaatgcg taagatgaaa gcaatacagt cttatcattt tccgtcaggc tcgcctcttc
     2821 actaaaccaa gagacatagt ttgtcagatt gcgatgctca atctgcacgc ccttcggctt
     2881 tccggtcgta ccggacgtat aaatgacgta tgcaagattg cgagacccgg aaatggatga
     2941 tgggcgctcc ttgctttcat ttctccattt accgtcatca aggctgacga ttgtcccttc
     3001 atattgccca atagccgtca tgtttctttc attcacgacc gcaaattttg ctccgctgtc
     3061 ctctaatgaa taacggattc gttcttcagg tgtatcagga tcaatcggaa ggaaagctgc
     3121 ccccgccttc atgacaccga gaatggatac catgacttca gctgaccggt ccataacaat
     3181 cgcgacaatg tcttcatttc ctgccccttg ggcacgaaga tgatgcgcca gctggttcgc
     3241 ctgttcatca agctcgcgat aggtcagcag atttccgcca gccttcaccg cagaatggtc
     3301 aggtgtttgc tgggcctgtt tttcaaaaag tgctgcaaag gttgcttcgg taatttgagt
     3361 catatgtctt tcttctagca aagcagccgt ttctgcagat gaaagcagat ctacatcaga
     3421 cagccgaacg ttcggattag ccgcggccgc tttgatgata ttgacaaagt ggcggctcca
     3481 gcggcggatc gtctcgtcct taaacaaggc agtcgcatag tcaaagctta ggccgatatc
     3541 ctcttcacgc tcgaccgctt ccaaggaaag atcaaatttc gcaacatgat gaagcatgga
     3601 atacgaggaa atcttcaaat ctcctaatct taatgaagga atctccatat tctgcatgtt
     3661 gaacatcacg ctgaacagcg gacttctgct tgtatccctt gtaagcggaa gcttttcaat
     3721 cagctcctca agcggatagc tctggtgctc gaatgcttga aggctggctg ttttgacctc
     3781 ttcaaggaat tgcgcgaagg ttttatctcc cgctggtgcc gttctgagtg ccaccgtgtt
     3841 gacaaacata cccggcacac cttgcagatc aggatgtgtt ctgccagctg tcggcgagcc
     3901 gacaatgata tcatcctgtg acgcgtactt ggaaaggagt acattgaacg ccgccagcaa
     3961 aaacatgtac attgtcgtat ctgtttctgc catgagcgat ttgatttgag ccgtgatttg
     4021 cttatcaagc ccaaacatca cgcgctctcc cgcaaagctc cgttcagctg gccgctcgaa
     4081 atccgcggga agatccagta ttggcagttc gcctttaaag acatcgagcc agtattcctc
     4141 atctttttga taattagttt gttctttgtg ccaaactgcg taatctttat agtgaatttg
     4201 tggcagttcc agatctgcgc cttgatatat tttggcaaga tcaccgatta gaatgcctgt
     4261 tgaacttccg tcagtaataa tatgatgcat atcaatgagc agcaggtggc gtttttcttc
     4321 cagctggaca agcttcgaac ggacgagcgg cgcgcggttt aattcaaacg gctgaacaaa
     4381 tgctttgatt atctcttccg cgtcttcttc ccgtcccttg gcagcctcca gctggaacga
     4441 tatgttttta tgaacggttt gcacaacctc tccgtcgatc atgtcaaacg atgtacggag
     4501 gatttcgtgc cggttgatta attgctgaat cgcgttttca agccggtctt tatctacttc
     4561 tccctccagc agaagtacag cggggacgtt gtagcttgtg tttgcttgtc caagctgatt
     4621 gaggatatac attctccgct gggccgggga tacaggataa tgctgctgat aagatgcttg
     4681 cctgatcggt tcaaacgtct gctcctcctt gctttcgttc tcttccaaat acagtgccag
     4741 ttcttgaata gtcggctttt caaaaagcac ttttatcgga acttccttat gaaaatgctc
     4801 ttgaattttc gccgtcatca tcatcgcttt caaagaatgg ccgccgatca taaagaagtt
     4861 atcggtaacg cctgctctga cgccgagtat tccttcccag atctccgcta atttcttttc
     4921 actctctgtt tctggtgctg cgtattccgg ctgcacggct tctgattgcg gtgcaggcag
     4981 ttctttttta ttgattttcc cgtttggcgt gagcggcagg ctgtccattt gaatcaggtg
     5041 ggcagggacc atatagtcag gcaaagactg agacagtgtt tttctcagct cgctcacagt
     5101 cacttcacga tccgccgtga agtacgcgca aatttcattt tcatccgctt tgttcttgcg
     5161 gatgatcacc gcggcttctg tcacatgctc agccatgttc agttttgttt caatttcgcc
     5221 aagctcaatt cggaagccgc gcaccttcac ttggttgtcg attctgccta aaaattcgat
     5281 attgccgtcc ggcagccatc ttgctaagtc accagtgcgg taaattctct cacccggtct
     5341 gaacgggtct tcgagaaatt gcttctccgt cagctcaggg agattgacat acccacgcgc
     5401 tacaccgatt ccgcctacgc aaagctcacc aggcgcgccg attggctgca ggcattgctg
     5461 ctcatccata atataggcag tggaatttcc gatcggcttc ccgattggga tagagccgcc
     5521 atactcgcgg tcgatcagaa aacttgttga aaacgtcgtg ttttctgtcg ggccgtatcc
     5581 gttccacaat gaaagcgacg gcgatgcctg ttttactttg ctgacaatat gcgggacaag
     5641 ggcatctccg ccgatgatta aatggcgcag tgtgccgaac atacccgcac cctttgcagc
     5701 aagctggttg aataaaggtg atgtcagcca catcgtcgag attctttgct cacgcaggaa
     5761 tgcagcaaat tgtttcgcat caagcagcgt ctcttttttg actggataaa gcgctgcgcc
     5821 attcagcagt gcgccgaata cctcaaacgt tccggcatca aagctgacag ctcccgtttg
     5881 cgccatgcgg tcctcttcag taacaggaac gtaccctgca tttttgacga ggcgcagaat
     5941 gcttttatgt tcgatcatga cgcctttcgg tttgcccgtt gaaccggatg tgtacatgat
     6001 gtacgccaaa tcagacgggg cggatggcag gctgagattg tcagccgctt cacgctcttc
     6061 aaaacctatg tccagtgtaa gaatcggcac ttgcagctga tgcgcctgcc ctgtataaga
     6121 atttccgatc aaaaccattc gaacggagct gtcctctgcc atgaaacgaa gacgatcctc
     6181 aggaagcgct ggatcaagcg gcacatatgc ggcacctgct tttaacacag ccagcatgcc
     6241 gacaacggtt tctactgagc gatcgttcat aatcgctgct gtttcccctt tttgaaggcc
     6301 ttgttcgaca agctttctcg ccagacggtt agcggctgca ttcagctcgc ggtacgtcag
     6361 ctcggttcct tcatatacga cggcagtgct cgccggcgtc cgtttcgctt gctcttcaaa
     6421 cacttggtga acagccattc cttcagggag ttcggctttc gtgctgttaa acacctcgac
     6481 aatttgccgc tgctcttcgt ctccaacaag cccgtactca cgcacaaaag cggccggctg
     6541 atcgacagct gcttccatca tgcgggtaag gtgctccgct gtccgttcga taaaagcgga
     6601 atcaaaggcg gctccgttat atttaatttt gacggtccat gttttgcccg gatacacaat
     6661 caggttgaaa tcgaagctcg tttgttcaaa tgcgctttct accttaattg aaaacccgag
     6721 gcggtcttcc atgctgccgt tttcaagctc ttgatcaagc ggataatttt caaacgcgac
     6781 cagatggttt aagagattgc catcaagagc tgagcgtttt tgaatctcat agagcggcac
     6841 gtaatcgtaa cgctctgcct ctactgcatg ctgctgaaca gctgtgaaaa tatcagcgaa
     6901 tgcagcatct cgttcaactt tcacccgcac cggaatggtg ttgataaaca gccccgccat
     6961 cgtttcaatt ccgttgattt cagacggcct tcctgagacg accgttccga agacaacatc
     7021 atccgtaaag ttgtatttgc tgagaacaat gccccaaacg gcctgaaata ggttaggccc
     7081 tgtcacttga tggagatttg cggtttgctg aatacgggca accaatgttt cgtcccatgt
     7141 aaaggaatat tctttattga cgtattcgtc tttttttaca ggcaggcggc ccgggagcac
     7201 gcttggctgt tcaaaacccg ccaagcgctc gctccagtag ctctccgctt cttcattgtc
     7261 ctgttttcca agccatttga tgtagtcgga gtaaggcttg ccttgaccgt ttgcaggtgt
     7321 ccttcctgct ctgagcgctt catagttttg gaacaggctt ttcatgagga cgcccatgct
     7381 ccagccgtcc atcataatat gatgattgct ccagacaaga tacagctgat ctgcatcaag
     7441 gcggaaaacc gctaccttga acaatatgtc ttttgccagg ttaaagcctt ggcgctgaac
     7501 gtcttgtttg taacgctcaa tgtgctcttt ctggcggttt tcgtctgcat gactgatatc
     7561 ctcataatgc acctttgttt tgcgttccgc taacacgacc tgacgcggtt tttgcagctg
     7621 ctgatgcaca aataccgtac ggagaatatc gtatgaccga atcagttcat tgacgctttt
     7681 ctcaaagatt tcaagatcaa aagcgccgtg aatcccaagc tctaattgtg tgaagtacga
     7741 ggaagaatgc ggatcaagca tcgcatgata cagcattccc tcctgcattg gtgtcagtgc
     7801 gtacaccttt tgaatcgatt ttttgctcat atgccacctc tattagaaaa tttccattaa
     7861 tttatccagc tcatccagcg tcaaatcgtc gtcaccaaga tcactcgggg tcagttccgt
     7921 accgtcttga gataggcaat gttcaatgat tctgagaaga tgcgctttaa agctgtcact
     7981 gaatgtttgt actgtttttt catgaaattc aagagaatga taggccagtg acatcgtcag
     8041 ttttccgttt tcgatatatc cgacaacatc cagcgcattc ggtttttctg tttcagggct
     8101 taatgactgt cctgatggca gctctgatct cgtaaacaaa cctgcatccg acatttcgtc
     8161 aaactgacct aaatagttga agctgacatc cggctgaatc gagaacgcca catcttcttt
     8221 atgttccggt gccgtcagat agcgcagaat gccgtatccg actcccttat tcggcacatg
     8281 tctgatatct tctttcattt gcttcagctg gtagcccaga tcatccgcat gcttcatatc
     8341 gagcaccatt gggtacatac ttgtaaacca gccgactgtg cgagaaatgg tcaggtcttc
     8401 tatgatttct tcacgtccat gcccctctaa atgaacactt actcgatctt gacccgtcca
     8461 ctccttcatt gccaatccga atgccgtcag caaaatatca ttcatttctg tgtgatatgc
     8521 ctcatgcacc tttgtggtga gctgttctgt ctcttcagca gtcagcgaga attcaattgt
     8581 ctttgtatgt ttcattcgct gatcaccaga ttcgcggtct ttcggaagct ttgccgcgac
     8641 tgcttgttct tcaagctgtc tccaatactc tttttctttc aaaaaggctc tggagttcgc
     8701 gaagtcttgc aatctctctg catactctgc aaatgaatgt gttttctgcg ggagaaccgg
     8761 ttcattgcct tgctcaagct gtgtatatac cgcggcgaaa tcctccagta aaatccgcca
     8821 cgacacgccg tcaaccacta agtggtgaat ggcaagaagc aagtgatctc cgtcttccgc
     8881 ttggaacaag cctgctttta acaagccgcc ttcctgcaag tcgatgctgc tttgcaaacg
     8941 ctccgcttct ctctctatac ggtcttcgta atctgcagct tggccctcaa tctggatgat
     9001 ctccaattga gcggaagagg cttcaagtcc tctgttgtat tgaacgacat cttcctgtcc
     9061 ctcacggtaa accatacgga gcgcgtcatg atgctcgatc agcgcctgca atgttttctc
     9121 cacccgttca gggtcaaagc ccttcgcggc gtaaagcatc accgattggt tccagtggtg
     9181 cttgttcgtg aagttctttt caaagaacca gcgctggatc ggcgtcagga tgacttcgcc
     9241 ctccaccggg ccttggtctg cctgtttgcc ttcggcacgc tctacgtact gggtgagctc
     9301 ttcgatcgtc ggatgctgga agagatcttt catttccagc ttccagccgt gctgattcaa
     9361 gcggctcgcc atttgaattc ctttgatgga atctccgcca agcgagaaga aattgtcggt
     9421 ga
//
