LOCUS       NZ_SHOB01000005        22954 bp    DNA     linear   CON 23-JUN-2024
DEFINITION  Bacillus subtilis strain A1 NODE_5_length_204591_cov_292.348604,
            whole genome shotgun sequence.
ACCESSION   NZ_SHOB01000005
VERSION     NZ_SHOB01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 204591)
  AUTHORS   Babalola,O., Ayangbenro,A.S. and Olanrewaju,O.S.
  TITLE     Draft genome sequence of three rhizospheric plant growth promoting
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 204591)
  AUTHORS   Babalola,O., Ayangbenro,A.S. and Olanrewaju,O.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2019) Biological Sciences, North-West University,
            South Africa, Corner Albert Luthuli and University Drive, Mafikeng,
            North West Private Bag X2046, South Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. DECEMBER-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 703.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004294565.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 03:44:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,243
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 4,031
            CDSs (with protein)               :: 4,031
            Genes (RNA)                       :: 103
            rRNAs                             :: 8, 6, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 6, 3 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 75 of 109
            Pseudo Genes (incomplete)         :: 50 of 109
            Pseudo Genes (internal stop)      :: 23 of 109
            Pseudo Genes (multiple problems)  :: 33 of 109
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:31:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 153997
            Orig. end                         :: 176951
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SHOB01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22954
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="sactipeptide"
                     /product="ranthipeptide"
                     /region_number="2"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /tool="antismash"
     cand_cluster    1..22954
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /kind="interleaved"
                     /product="sactipeptide"
                     /product="ranthipeptide"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..22954
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12954](-)"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12954)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     misc_feature    complement(88..102)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(548..561)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.64"
                     /tool="antismash"
     misc_feature    complement(567..581)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.38"
                     /tool="antismash"
     gene            complement(642..1826)
                     /gene="ybdO"
                     /locus_tag="ES060_RS20130"
                     /old_locus_tag="ES060_20130"
     CDS             complement(642..1826)
                     /codon_start=1
                     /gene="ybdO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388087.1"
                     /locus_tag="ES060_RS20130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20130"
                     /product="DUF4885 domain-containing protein"
                     /protein_id="WP_130573422.1"
                     /transl_table=11
                     /translation="MRLNRQFIRTQLMAQNILSKNAPAKRENATENPAAVLEKAYSRLK
                     SQSSTGGINQFNYSKTSVSGNSGAFSKVYQSANDRTVTDTGEETVIQSQNPYESESDIR
                     IKILDEKYSRMNAINKTKSDPLGYIKDKYQNSKSPYFRSDLSAAERQAAYDNETEWLFK
                     GKAQNYNLQDAAFRNVTFHGEVEAENEKVYQRGQVNQQLQVLLNRNHIYIPEGTELTFT
                     ITPIDYQVRVSGTDDQDLIKQIEQVLQSGDNSKELFLHIMKSQSSDSTQFSEEAYQKYQ
                     AAREMYEVTGYHLKDLEVIDGRYVTPDGRDLIDVYKEELEKDPVQKQTASYAISYYRSE
                     LSKIAEAGYNAIPDFILSIDYSNGSLRDVGQSKSYGTGDTGWLEALKRQTGVNY"
     protocluster    1488..22720
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[11487:12720](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(11488..12720)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="2"
     misc_feature    complement(1864..1878)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     misc_feature    complement(1892..1901)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            1958..2815
                     /locus_tag="ES060_RS20135"
                     /old_locus_tag="ES060_20135"
     CDS             1958..2815
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388086.1"
                     /locus_tag="ES060_RS20135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20135"
                     /product="hypothetical protein"
                     /protein_id="WP_024571601.1"
                     /transl_table=11
                     /translation="MVKKWLIPFAVMLSVLSTFTYSASAVGVTAITGNNSFDTAMPIGY
                     WKYKNIDTTILEAGQDEAYFTFTANKGEKVYVRSTYQSAYTGMKIEIYDKNRIPVSQGT
                     EVINPNTFSSFIYANADAQNTTDTFYVKVSRGTYTGNMYFTLSIEDRIKSGSGTFQFSG
                     VAENKGNTSLSPSGSDSSVIKVDLTNQSGIPRDAIVTRVQTTATQTPSQGNTRHLIMTS
                     ENNEWRRALVNSSTSGSYDISLSDQLSVAKVWSFKYNTLATARSTMSNVKAKIDYEYDV
                     TKQF"
     gene            2901..3671
                     /gene="pkrD"
                     /locus_tag="ES060_RS20140"
                     /old_locus_tag="ES060_20140"
     CDS             2901..3671
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="pkrD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388085.1"
                     /locus_tag="ES060_RS20140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20140"
                     /product="protein kinase"
                     /protein_id="WP_024571602.1"
                     /sec_met_domain="Pkinase (E-value: 1.2e-33, bitscore:
                     110.2, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALKLLKKLLFDRPLKNGVILNHQYKIEECLGMGGYGLVYLCTDI
                     LTQTPYVLKQLRPTKAKKEKEKVRFQQEIKLLKSIHHPQIPGFIDEFIIDGQAYYVMQF
                     IEGENIEELLFFRKQPFTELMALQLISQLLEIIEYLHDRLIFHSDIRTPNIIINDGRLC
                     LIDFGLAKQLTPEEMEEIKVRKQDDFFDLGETLLFLLYSQYKGKKKKNGTWLEELTLTK
                     EVTLLLKRLLGIEEEYQHTASIREDLNRAIQSVT"
     gene            complement(3656..3919)
                     /locus_tag="ES060_RS20145"
                     /old_locus_tag="ES060_20145"
     CDS             complement(3656..3919)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003097672.1"
                     /locus_tag="ES060_RS20145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20145"
                     /product="hypothetical protein"
                     /protein_id="WP_024571603.1"
                     /transl_table=11
                     /translation="MNSICLKKSSKRLSTSQAVLVTDIADTGITAAVGADIIAATAAVR
                     AKEDTTTVMAAVIVTNAGATAAVNPKKVLVPLLGQGFFYVTD"
     gene            complement(3978..4964)
                     /gene="ybdK"
                     /locus_tag="ES060_RS20150"
                     /old_locus_tag="ES060_20150"
     CDS             complement(3978..4964)
                     /codon_start=1
                     /gene="ybdK"
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234894.1"
                     /locus_tag="ES060_RS20150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20150"
                     /product="two-component system sensor histidine kinase
                     YbdK"
                     /protein_id="WP_169072855.1"
                     /transl_table=11
                     /translation="MKLKTKYQLLLFTAVISVPMLLLAVSVLMSVIYDSMFKTMNHGMP
                     FHRSFAYPAMIVVFLISLLLLAFLFSKSIHSLLHKINLLNQTIRHLASDQRVPDKIEVK
                     RADEIGELIKSVNLLIERTTYRELELRQQEEIKKELLQKLRHDINTPLTALRLQLFYLE
                     DQCHGQAVFESLYQQIEYISELTNEFNLYSAETLESSYIVNEEVRLNELLETAVKKWDY
                     LYSMSGIELHYKPADQDVIWMSNALWMQRLFDNIFQNTLRHSKAEKMEVTIEHGDVFIR
                     DDGIGFDRNESSEGLGLKIIEDTCRLLAITYELHTNDNGTGFLFSKE"
     gene            complement(4961..5632)
                     /gene="ybdJ"
                     /locus_tag="ES060_RS20155"
                     /old_locus_tag="ES060_20155"
     CDS             complement(4961..5632)
                     /codon_start=1
                     /gene="ybdJ"
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388082.1"
                     /locus_tag="ES060_RS20155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20155"
                     /product="two-component system response regulator YbdJ"
                     /protein_id="WP_015382624.1"
                     /transl_table=11
                     /translation="MKGYHILIVEDDVMIGDLLQKILQREGYRVIWKTDGADVLSVIQK
                     VDLVIMDVMLPGEDGYQMSAKIKELGLGIPVIFLSARNDMDSKLQGLQIGEDYMVKPFD
                     PRELLLRMRNMLEHHYGTFTQIKHLYIDAVTKKVFNESLHDEVLFTAIERKIFFYLYEN
                     RDSILTKEHFFEYLWQLEDRNPNIVNVHIKKIRAKINDQAGEMIENIYGEGYRLNTVVK
                     K"
     gene            complement(5634..6611)
                     /gene="ybdG"
                     /locus_tag="ES060_RS20160"
                     /old_locus_tag="ES060_20160"
     CDS             complement(5634..6611)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybdG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388081.2"
                     /locus_tag="ES060_RS20160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20160"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_130573423.1"
                     /sec_met_domain="PF00561 (E-value: 1.4e-07, bitscore: 25.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTLWKVLKIVFVSLAALVLLVSVSVFIYHHFQLNKEAALLKEKG
                     TLVEVDGKKMNVYQEGSGKDTFVFMSGSGIAAPAYEMKGLYSKFSKENKIAVVDRAGYG
                     YSEVSHDDRDIDTVLEQTRKALMKSGNKPPYILMPHSISGIEAMYWVQKYPKEIKAIIA
                     MDIGLPQQYVTYKLSGVDRLKVRGFHLLTSIGFHRFIPSAVYNPEVIRQSFLTDEEKEI
                     YKAINFKQFFNADMEHELLQSYQNGSKSVNLPAPKETPVLILDAVSDQNRHSKYAIQNR
                     KDYEAFAAQFNTADIKELRGTHSIYLYQPDQIYKLSMEFMRKVR"
     gene            complement(6860..7285)
                     /gene="skfH"
                     /locus_tag="ES060_RS20165"
                     /old_locus_tag="ES060_20165"
     CDS             complement(6860..7285)
                     /codon_start=1
                     /gene="skfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388080.2"
                     /locus_tag="ES060_RS20165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20165"
                     /product="sporulation killing factor biosynthesis/export
                     protein SkfH"
                     /protein_id="WP_003246390.1"
                     /transl_table=11
                     /translation="MKDEQMLTEWPSHLPWLNQSQNDFTFPSDTYLLLYFWSMSCPNCH
                     QLTDKVLQDIKDMNVKVIGVHVPYIEEEKSMEVVLTYALDRGLAIPIVLDQNYEIVTTC
                     HVQGIPSFCLLSQYGQIITKTMGDVGWDKMLKKIAGL"
     misc_feature    complement(7252..7267)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.76"
                     /tool="antismash"
     gene            complement(7290..7805)
                     /gene="skfG"
                     /locus_tag="ES060_RS20170"
                     /old_locus_tag="ES060_20170"
     CDS             complement(7290..7805)
                     /codon_start=1
                     /gene="skfG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388079.2"
                     /locus_tag="ES060_RS20170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20170"
                     /product="sporulation killing factor biosynthesis/export
                     protein SkfG"
                     /protein_id="WP_003234897.1"
                     /transl_table=11
                     /translation="MNSNGDKLSLSVQNLANTNEITIVQAIGELKKSGKDAIPVLVEAL
                     KEEGSLCNIAAAVLGEFGEDASEAAEELSCLLKSHAEDTRMAAAISLMRIGKPSLPFVI
                     KIAQESEGQSCFWASWCIAWIDPSCIEPKMYKCLKYEHEHPSGIVAPFAAEEALGKLIA
                     FQLKDKED"
     gene            complement(7852..9267)
                     /gene="skfF"
                     /locus_tag="ES060_RS20175"
                     /old_locus_tag="ES060_20175"
     CDS             complement(7852..9267)
                     /codon_start=1
                     /gene="skfF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019257319.1"
                     /locus_tag="ES060_RS20175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20175"
                     /product="sporulation killing factor system integral
                     membrane protein"
                     /protein_id="WP_130573424.1"
                     /transl_table=11
                     /translation="MNNIISFLWLQSKRRFVSQGQEKKMPFLIMLLFVGAIGFQVSFVS
                     RSTTWDMSIAGWVLTGVFILYTAFGLFSNRLPSQMADIIWLYGTATSFSKVVYSVLFFS
                     VTWKALLWIISAIFGDVLIVLLSGDHINLLGRSIIFVGLFFIAEVWLMSVSCARTVKKM
                     KRVYVLVFLLMLGIYSICLYHFFFLQHSSGIWESIARFISGVGLVFDTLSPLYVVVFIG
                     IITVSFMTIAFTSRQVEMKESLVKEAEFWEEFQERQFGSGQIIQKPKTTWWGLQGLNGI
                     WSFLWLELLLFKKYLFFHSIHTVMLSGVFYVVIFMYPEWFYLLFFLIVSAVMLSSYYSG
                     IVRHSQSGTLHLFPGALWKKIIILELTNTVWLYILYCVSITFMAVGNLVYWYIYGLGIY
                     IWFMTIRLFAFTHTNRNDIKLSLPQYYKSFFMALGLSGICLYVIHLLTADWYTLVVVVC
                     IGSLSWCLFYRFR"
     gene            complement(9260..9982)
                     /gene="skfE"
                     /locus_tag="ES060_RS20180"
                     /old_locus_tag="ES060_20180"
     CDS             complement(9260..9982)
                     /codon_start=1
                     /gene="skfE"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388077.1"
                     /locus_tag="ES060_RS20180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20180"
                     /product="sporulation killing factor ABC transporter
                     ATP-binding protein SkfE"
                     /protein_id="WP_017694958.1"
                     /transl_table=11
                     /translation="MQLMQVQNLSKCYRNGDGVEHLSFSIQRGEIVALLGPNGAGKTTT
                     IRCLTGLYKPDKGDILIEGSPPGHINVQKKVALIPDQPYLYPALTAAEHIQFRARGYHP
                     GKKDVKERVYHALKEVHLEEKANQLCGQLSRGQKQRVVLAGAIVQDALLYILDEPTVGL
                     DIPSKQWLSNWLKTKTDQGCSAFVSTHSLEFVLETADRVILIRDGKLMQDLYVPQFEEE
                     QAEWRKEVIRLLGEWSDE"
     gene            complement(10001..11491)
                     /gene="skfC"
                     /locus_tag="ES060_RS20185"
                     /old_locus_tag="ES060_20185"
     CDS             complement(10001..11491)
                     /codon_start=1
                     /gene="skfC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: skfc"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003097671.1"
                     /locus_tag="ES060_RS20185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20185"
                     /product="sporulation killing factor biosynthesis protein
                     SkfC"
                     /protein_id="WP_130573425.1"
                     /sec_met_domain="skfc (E-value: 2.4e-288, bitscore: 950.6,
                     seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="Abi (E-value: 4.4e-14, bitscore: 46.3,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSLSLVFWSILAVVGLLLFIKFKPPTIASLLLSKDEAKEISIQF
                     IKEFVGIDVENWDFYSVYWYDHDTVNKLHHLGILKKNRKVLYDVGLVESWRVRFVHKNQ
                     SFVVGVNANREITFFYADVPKKSLSEKFEQVSPGTLKQRLMASPDGLWSRANMTGTGKK
                     EEDFREVSTYWYIAEAGDIRLKVTVELQGGRISYIGTEQEILTDQMSKVIRDEQVESTF
                     GVSGMLGSALAMILAILILVFMDVQTSIIFSLVLGLLIIICQSLTLKEDIQLTIVNAYD
                     ARMSVKTVSLLGILSTLLTGLLTGFVVFICSLAGNALAGDFGWKTFEQPIVQIFYGIGA
                     GLISLGVTSLLFNLLEKKHYLRISPELSNRTVFLSGFTFRQGLNMSIQSSIGEEVIYRL
                     LMIPVIWWMSGNILISIIVSSFLWAVMHQVTGYDPRWIRWLHLFIFGCFLGVLFIKFGF
                     ICVLVAHFIHNLVLVCMPLWQFKLQKHMHHDQPKHTSL"
     gene            complement(11488..12720)
                     /gene="skfB"
                     /locus_tag="ES060_RS20190"
                     /old_locus_tag="ES060_20190"
     CDS             complement(11488..12720)
                     /codon_start=1
                     /gene="skfB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Other_Sactipeptide_rSAM_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388073.2"
                     /locus_tag="ES060_RS20190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20190"
                     /product="sporulation killing factor system radical SAM
                     maturase"
                     /protein_id="WP_003234902.1"
                     /sec_met_domain="Other_Sactipeptide_rSAM_RRE (E-value:
                     1.2e-15, bitscore: 51.4, seeds: 13, tool:
                     rule-based-clusters)"
                     /sec_met_domain="PTHR43273 (E-value: 3.5e-32, bitscore:
                     105.3, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.2e-22, bitscore: 74.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 2.2e-07, bitscore: 24.8,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.3e-06, bitscore: 21.7,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYDRVKDFDLPELAVHLQPHGAVMIDRKSMFYFRLSGRGAQLAF
                     LLSKNKNLHKTARIWEIMKKEEMSADQLKEELSAHPFTEAWTEGLLDQPLHVSGSLDSY
                     LPISCTLQLTNACNLSCSFCYASSGKPYPEELSSEQWILVMQKLAAHGVADITLTGGEA
                     KLIKGFKELVVVASSLFTNVNVFSNGLNWRDEEVELLSHLGNVSVQISIDGMDNTHDQL
                     RGRKGGFKESMNTIKKLSEANIPVIVAMTINESNADEVSDVVEQCANAGAFIFRAGKTL
                     SVGRATEGFKALDIDFEEMVQIQLREARHKWGDRLNIIDWEHEESSFTTDFCTPGYLAW
                     YIRADGYVTPCQLEDLPLGHILEDSMADIGSPARLLQLKCEAKNCKCIGKIELSEPDLP
                     FQKEVKAGIQE"
     aSDomain        complement(12418..12720)
                     /aSDomain="Other_Sactipeptide_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a sactipeptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_ES060_RS20190_Other_Sactipeptide_rSAM
                     _RRE.1"
                     /evalue="1.70E-16"
                     /identifier="RREFam021.1"
                     /label="ES060_RS20190"
                     /locus_tag="ES060_RS20190"
                     /protein_end="101"
                     /protein_start="0"
                     /score="51.4"
                     /tool="antismash"
                     /translation="MSYDRVKDFDLPELAVHLQPHGAVMIDRKSMFYFRLSGRGAQLAF
                     LLSKNKNLHKTARIWEIMKKEEMSADQLKEELSAHPFTEAWTEGLLDQPLHVSGSL"
     gene            complement(12787..12954)
                     /gene="skfA"
                     /locus_tag="ES060_RS20195"
                     /old_locus_tag="ES060_20195"
     CDS             complement(12787..12954)
                     /codon_start=1
                     /gene="skfA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: TIGR04404"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388072.1"
                     /locus_tag="ES060_RS20195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20195"
                     /product="sporulation killing factor"
                     /protein_id="WP_009966416.1"
                     /sec_met_domain="TIGR04404 (E-value: 2.1e-38, bitscore:
                     123.7, seeds: 3, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRNQKEWESVSKKGLMKPGGTSIVKAAGCMGCWASKSIAMTRVC
                     ALPHPAMRAI"
     gene            complement(13426..>13768)
                     /locus_tag="ES060_RS21655"
                     /old_locus_tag="ES060_20200"
                     /pseudo=""
     CDS             complement(13426..>13768)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003223659.1"
                     /locus_tag="ES060_RS21655"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="ES060_20200"
                     /product="glutamine--fructose-6-phosphate aminotransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="VCVEGALKLKGIS"
     gene            complement(13869..15041)
                     /gene="ybcL"
                     /locus_tag="ES060_RS20205"
                     /old_locus_tag="ES060_20205"
     CDS             complement(13869..15041)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybcL"
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388070.1"
                     /locus_tag="ES060_RS20205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20205"
                     /product="MFS transporter"
                     /protein_id="WP_130573426.1"
                     /transl_table=11
                     /translation="MSNTWKIYILAIVSFLVGTSEYIISGILDQIAHTLGITLAAAGQL
                     ITIFSLVYALSTPVLMALTASMDRRKLMMYALGLFVFGNVLAFVLPGYGWFIAARIIMA
                     MGAGVVVVTALTIAAKIASEGKQGSAIATVVMGFTASLIIGVPLGRMIAVALGWKSVFG
                     AIALLGLIAMVVIFFTLPHTEGDKPVPLLQQLALFKKRKVAMGLSITFFWLGGYSVAYT
                     YLSPYLLNISGINGKLLSGVLLIFGIASLVGSKFGGYSTDKWGVPFTLVGGMTLHIVTL
                     ILLSLVTHSYIGVLVILILWSFAAWSTGPTQQFHLATIEPEMSGVLLSMNQSMMQFAMA
                     VGAGIGGVFVENVSLASITWVGALGVMIAIITSLTIFHSQPKQTLKDINQ"
     gene            complement(15168..15470)
                     /gene="ybzH"
                     /locus_tag="ES060_RS20215"
                     /old_locus_tag="ES060_20215"
     CDS             complement(15168..15470)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="ybzH"
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003097670.1"
                     /locus_tag="ES060_RS20215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20215"
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_003234907.1"
                     /transl_table=11
                     /translation="MEPIEVFKALSNESRLQILQWLKEPDRHFAPHEGIDMNTIGVCVS
                     QITDKLKMTQSTASQYLTILLRAGLIKAERIGKYTYYKRDEEAIGKLADFLKTEI"
     gene            complement(15945..16307)
                     /gene="ybcI"
                     /locus_tag="ES060_RS20220"
                     /old_locus_tag="ES060_20220"
     CDS             complement(15945..16307)
                     /codon_start=1
                     /gene="ybcI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019257313.1"
                     /locus_tag="ES060_RS20220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20220"
                     /product="DUF2294 domain-containing protein"
                     /protein_id="WP_015715144.1"
                     /transl_table=11
                     /translation="MKKSKGSIESEISKSITQWEKDYLGRGSVSVKTDILRDMIIVNLK
                     GILTPAEYVVCGSKEGMLSIKQTRSELVESGIEGLKDIILKITGEKVKSFHTDLSSRTG
                     ERVMVFKLCNDLEKNL"
     gene            complement(16364..16654)
                     /gene="ybcH"
                     /locus_tag="ES060_RS20225"
                     /old_locus_tag="ES060_20225"
     CDS             complement(16364..16654)
                     /codon_start=1
                     /gene="ybcH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388068.1"
                     /locus_tag="ES060_RS20225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20225"
                     /product="DUF6407 family protein"
                     /protein_id="WP_014475635.1"
                     /transl_table=11
                     /translation="MSANLTDFVTKTIEEMNSFDRENMECMKKLIRKAIDFYHLKSYEE
                     VEETHSGNVRFLHVHSMMEENMLSKMIVVTRNGKTDLDIEGVYEGYVVREY"
     gene            complement(16718..17245)
                     /gene="ybcF"
                     /locus_tag="ES060_RS20230"
                     /old_locus_tag="ES060_20230"
     CDS             complement(16718..17245)
                     /codon_start=1
                     /gene="ybcF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388067.1"
                     /locus_tag="ES060_RS20230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20230"
                     /product="carbonic anhydrase"
                     /protein_id="WP_130573427.1"
                     /transl_table=11
                     /translation="MNVNQNKKVLFLTDIENGLEPILQEATNTSAENMLTIQSYGAGIS
                     HPYGEIMRSVIFAIYQEDVEEIFVVGTKDKKTSAGNELTQLETMKDKIQTLDYLFQNCK
                     PEFLGGTVDEWLNENSSDTIEKSVDMIRHHPLVPSHVKVRGLLVNHKDGKPSIMEGPDA
                     KTGQAIPDHCLS"
     gene            complement(17322..19937)
                     /gene="mpsB"
                     /locus_tag="ES060_RS20235"
                     /old_locus_tag="ES060_20235"
     CDS             complement(17322..19937)
                     /codon_start=1
                     /gene="mpsB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388065.2"
                     /locus_tag="ES060_RS20235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20235"
                     /product="YbcC family protein"
                     /protein_id="WP_130573428.1"
                     /transl_table=11
                     /translation="MGITSVLTKDNVKKIDTDIDVQERDLNVFITSASRVIAPLWPIST
                     FAARNPWMGLENQSFDQVASWLKNTRDVDIYPSASMIRSAKNKGEIDEDFVEMGLQRWL
                     DSHSYHIPRDVAERFCHAALKLDPLPSDLLSSHELEKLVGECSGLDHIENVFMQPLSSY
                     IENQDGERLVNILDHHVIKWSKLYLDDSQAGWTMPNREEGFYRAWQHLIQYDPALSKKQ
                     RERVKDWPKEAHLALQEALFALEIPESEIQTYLEGHLLSLPGWAGMMLWRSQQSSHEHA
                     LLTEYLAVRISMEWALIKPYLPLTNERSKKKISIAPLIAAWIHWGGLTLEEWSQMTAIE
                     QNEYLSFAYSFDEKLRKKLWLEAWEQTYTDRLSQKIISKQRETGGKKSALAQLAFCIDV
                     RSEPFRRQLEKEGPFETIGIAGFFGVPIATCELGSKHSHASLPIIQKPQNKIKEFADED
                     VFKKYNQRKQAIHSLSHTFKTMKQNALSSLLLPELSGPWLTLQMAARSFVPRKAGRFIR
                     NLREAWLRKPDTKLSLHHDTTEAEIPVGFTDEEKVNYARQALKMMGLTENIAPLVVICG
                     HGSQSTNNPYSAALDCGACGGAAGGFNARVLAALCNLSEVREALLAEGIQIPEDTVFAA
                     AEHNTTVDELHWLYVPELSEAAQEAFEQIEAVMPKVRHHVNAERLDQLPNFQSKLKNPK
                     AEANRFAEDWSEIRPEWGLARNAAFIIGKRELTQDCDLEGRAFLHNYDWKQDESGELLA
                     NIIAGPGTVAQWINLQYYASTVAPHYYGSGNKATQTVTAGLGVMQGNASDLLAGLPWQS
                     VMESDHEAYHSPLRLLILIQAPREYVERLLNHDSAFLQKVQNGWVRLASLDPEGCWESW
                     "
     gene            complement(19952..21469)
                     /gene="mpsA"
                     /locus_tag="ES060_RS20240"
                     /old_locus_tag="ES060_20240"
     CDS             complement(19952..21469)
                     /EC_number="1.6.5.9"
                     /codon_start=1
                     /gene="mpsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388064.1"
                     /locus_tag="ES060_RS20240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20240"
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="WP_130573429.1"
                     /transl_table=11
                     /translation="MLVSLSLSSLLTLFFIMLMASGISGLLFLHPRVPLSFVRIHIGIL
                     ALPLLVSLLILANSGVSGNVGPWHLDSLACLMTFFVLAIGFIIQRFSVRYLLGDRSYRK
                     YFTLFTFTTGAASMTWLSGDLRLMVLFWGATLVGLTLLIRLNSAWQVASEAAKVSGRLF
                     LLSWFSLFFAMMWLFHATGQWQLSLVVTNESLAGLGEWERTGIQLLIVLAVIIPAAQWP
                     FQRWLVESIVAPTPVSAIMHAGLVNAGGIILTRFSPLFHGGIASIVLLLLAGISVLIGT
                     GISLVQVDYKRQLVGSTIGQMGFMLIQCALGAYIAAIIHLILHGLFKATLFLQAGSAVG
                     RHEVSARTNERTSYLWVMAGRILSLVIGVAFWLAAPGEGYQFISALILGWSLSVSWTQL
                     VAFGEGRIGRIAGLTVLGGAALVYFIIHNLFYKWLHTTIFQSVQPPMSAVMIVVCLLLF
                     GSALGTWVARHRSSVFFAVLYLWLVRLGEAKPKSVESHPDYLKQYIS"
     gene            complement(21988..22530)
                     /gene="adaB"
                     /locus_tag="ES060_RS20245"
                     /old_locus_tag="ES060_20245"
     CDS             complement(21988..22530)
                     /codon_start=1
                     /gene="adaB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388063.1"
                     /locus_tag="ES060_RS20245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ES060_20245"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_130573430.1"
                     /transl_table=11
                     /translation="METKNKPTLYWSLLMFKDWNFYIASTLKGLVFVGSQNKPIEELFE
                     WARKRFPGSLLVEDDDKLEPYAVEITQYLEGKRKNFTVPVEYAGTQFQLAVWNALCEIP
                     YGQTKSYSDIANEINKPAAVRAVGAAIGANPVLITVPCHRVIGKNGSLTGYRGGFEMKT
                     LLLDLEKRASSEMDVPH"
ORIGIN
        1 agcgataatt tctgacatgc cgacgataat ccactggaac cagttgctcc atgctgttat
       61 ataacctgcc atcggatgaa tatattgatg gccgaacgtc gcaaaagaac ctgtgctcgg
      121 ctcgacatac agcatttcac ccatcgcacg cataataaaa aagataaaaa ttccgcatat
      181 tgcataagcc agaaggacag atgggccagt ccatgaaatc gtgctggcag aacccataaa
      241 caagccaaca ccgattgtac cgcccaaggc gatcatctga atatggcgcg cgcccaggcc
      301 cctctttaat tctttatttg ccactcctct tcctcctcct aacaaaatac tcataaatga
      361 gaaaacagca gacctacttg ccttcacatg aatcagtgat aacaatcaga aaattcaaac
      421 ttaaaattgt tatataatct cctaaattac ttatttcatc ataataaatt tctctggaaa
      481 atacaattgt ttttttcagg taaacacctt tgttcagagt tttgaatcgt tcgcttcttt
      541 taaaatcttt tatataaaaa taataaaatt gtttgaatat tgaaattaat acacccctta
      601 gattctcata aaaaataagc tgttctacat gaacagctta cttaataatt cacacccgtt
      661 tgacgcttta atgcttcaag ccacccggta tctccggtcc catagctctt gctttgtccc
      721 acatctctca gagatccatt gctgtaatca attgatagga tgaaatccgg aatggcatta
      781 tacccggctt ccgcgatttt actgagctct gaacgatagt aagatatagc ataagaagcc
      841 gtttgcttct gcactggatc tttttcaagc tcttccttat atacatctat taagtcacgt
      901 ccatcaggcg tgacgtaccg tccatcaatg acttctaaat ccttcaaatg gtagccagtg
      961 acttcgtaca tctcacgagc tgcctgatac ttttgatagg cctcttcgga aaattgtgtt
     1021 gaatcactgc tttggctttt catgatgtgc aaaaatagtt ctttgctgtt gtctccagat
     1081 tgcagcactt gctcaatttg cttgatcaga tcctgatcgt ctgtcccgct cacccgcacc
     1141 tgatagtcaa tcggtgtgat tgtaaacgtc agctctgtcc cctctggaat ataaatatgg
     1201 tttcgattca acagcacttg cagctgctgg ttaacctgtc cccgctgata caccttttca
     1261 ttctcagctt ccacttcccc atgaaatgtg acattccgaa acgcagcatc ctgaaggtta
     1321 tagttttggg ccttgccttt gaatagccat tctgtttcat tatcgtaagc ggcctgtctt
     1381 tctgcagctg acagatcact tctgaaataa ggagactttg aattttggta cttatcctta
     1441 atatagccta atggatcaga ctttgtttta ttaatcgcat tcatcctgct gtatttttca
     1501 tctagtattt tgattctgat atcactctca gattcatagg ggttttggga ttgaatcact
     1561 gtttcttccc ctgtgtctgt cactgttcga tcattcgctg attgatacac tttgctgaac
     1621 gccccgctgt ttcccgacac gctggtttta gaataattga actgattgat tcccccagtt
     1681 gaagattgtg acttaagcct gctgtatgcc ttttccagaa ctgccgctgg attttcagtc
     1741 gcattctccc gtttggcagg cgcattcttg ctcaatatgt tctgtgccat cagctgagtg
     1801 cggataaatt gccgattcag acgcattgaa ttccctcccg agtactctct cttattcttt
     1861 tttatcggca aagatgatgt atatattaac ctcaaaaaac atttatttac acctaaatcg
     1921 ttattatgta tgatggaaat aaaaggaggt tgtttgcatg gtgaaaaaat ggcttattcc
     1981 attcgctgtt atgctgtctg tcttatctac ctttacgtat tcggcatcag ctgtcggagt
     2041 cactgctatc acaggaaaca attcatttga taccgcaatg ccgatcggtt attggaaata
     2101 taaaaatatc gatacaacaa tactggaagc tggtcaagat gaagcctatt tcacttttac
     2161 ggctaataag ggagaaaaag tatacgtgag gagtacatac cagagcgcgt atacagggat
     2221 gaaaattgaa atttacgata agaatagaat acctgtttct caagggacag aagtcattaa
     2281 ccctaatacg ttttcttctt ttatttatgc aaatgcagac gcccaaaaca cgacagatac
     2341 cttttatgtg aaagtgtcac gaggcaccta taccggaaac atgtatttta cactttcaat
     2401 tgaagaccga atcaaatcag gcagcggaac gttccaattc agcggcgttg ctgaaaataa
     2461 aggaaataca agccttagcc cctcaggttc tgactccagc gtgatcaagg ttgatttaac
     2521 aaaccaatct gggataccgc gtgatgccat tgtaaccaga gttcagacaa ccgccactca
     2581 aacaccaagc cagggaaata cacgccacct gatcatgacg agcgaaaaca acgaatggcg
     2641 ccgtgctctt gtaaacagct cgacttcagg gtcttatgat atctctttgt cagatcaact
     2701 cagtgtggca aaggtatgga gttttaaata taatacatta gcgacagcca gatcgacgat
     2761 gagcaacgta aaagcaaaaa tagattatga atacgacgtg acaaagcagt tttaacttat
     2821 accgagccgg ttatccggct cttttttatt ctcagtctag aaggtaaact atagtcagta
     2881 caaccaaggg aagtgcagac atggcattaa aacttctaaa aaaactgcta tttgaccgcc
     2941 ctctaaagaa cggcgttatc ttaaatcatc aatacaaaat cgaagagtgc ctcggtatgg
     3001 gaggctacgg tttggtttat ttgtgcaccg atatcctaac tcaaacacct tatgtcttaa
     3061 aacagcttcg gccgacaaaa gcgaaaaagg aaaaagaaaa agtccggttc cagcaggaaa
     3121 taaaacttct caaaagcatt caccacccgc aaattccggg cttcatagat gagttcatca
     3181 tagacggaca ggcttattat gtcatgcagt tcattgaggg agagaatata gaagaattat
     3241 tattttttcg aaaacagccg ttcacagagc ttatggcgtt acaactgatt tcgcagctgc
     3301 ttgagattat agaatacctg catgatcgcc ttatcttcca cagcgacatc agaacgccta
     3361 atatcatcat caatgacggc aggctctgcc tcatcgattt cggccttgcc aaacaattga
     3421 ctcctgaaga aatggaggaa ataaaggtac gaaaacagga tgacttcttc gatctcggag
     3481 aaacgctgtt attcctgctg tactctcaat ataaaggaaa aaagaagaaa aacggcactt
     3541 ggctggagga attaacactg acaaaggaag taaccttgct gctgaaaaga ttattgggca
     3601 ttgaggaaga gtatcagcac accgcttcca ttcgcgagga tctaaaccgg gccattcaat
     3661 cggtcacata aaaaaaccct tgtcccaaaa gcggaacaag aacttttttt ggattaactg
     3721 ctgctgtagc tccggcgttt gtaacgatga ctgccgccat aacggtcgta gtgtcttctt
     3781 ttgcccgaac tgctgctgta gctgcgataa tgtctgcgcc gactgctgct gtaatgccgg
     3841 tgtctgcgat atcggtcact agaactgctt gagaggtact taatcgtttt gatgattttt
     3901 ttaaacatat gctgttcacc ccatttcttt ctccctatta tataaatttt ataggagaaa
     3961 gaaaaacatc agcggggtca ctcttttgaa aacaagaacc ccgttccgtt atcatttgta
     4021 tgcagctcat aagtaatcgc aagcagcctg catgtatcct caataatctt taacccaagt
     4081 ccctcactgc tctcattccg atcaaatcca ataccgtcat cacgaataaa aacatcgcca
     4141 tgttcaatcg tgacttccat cttttcagct tttgaatgcc ttaacgtatt ttgaaaaata
     4201 ttatcaaaca gcctttgcat ccataacgcg ttgctcatcc atatcacatc ttgatctgcc
     4261 ggcttatagt gcagctcaat cccactcata ctgtataaat aatcccactt tttcaccgct
     4321 gtttctaata gctcgtttag acgcacttct tcatttacaa tataagagct ttccagcgtc
     4381 tcagcggaat atagattgaa ttcattggtt aattccgaga tatattcgat ttgctgatac
     4441 aatgattcga atacagcctg accatgacat tggtcttcca aataaaataa ctgcagcctg
     4501 agagccgtta aaggtgtatt aatgtcatgc cgcagttttt gcaaaagctc ctttttgatt
     4561 tcctcttgct gtctcagctc cagttcacga tatgtcgtcc gttcaattaa caaattgacc
     4621 gacttgatca gttccccgat ttcatccgca cgcttcactt caattttatc gggcaccctt
     4681 tgatcactcg ccaaatgccg aatggtttga tttaatagat tgattttatg caacaaagaa
     4741 tgaatcgact ttgaaaataa aaaagctaac aataagagcg atatcaaaaa tacaacgatc
     4801 attgccgggt acgcaaacga cctgtgaaag ggcataccat gattcatcgt tttaaacatg
     4861 ctgtcataaa tcacagacat caaaacactg accgccagca atagcatcgg aacactaatg
     4921 acggccgtaa acaataacaa ctgatatttt gtcttgagct tcatttcttc acaacggtat
     4981 tcagccgata cccttctcca tatatatttt caatcatctc acccgcttga tcattgattt
     5041 tagctctgat ttttttaata tgcacattga caatattcgg gtttctatct tcgagctgcc
     5101 atagatattc aaagaaatgt tcctttgtca ggatactgtc tctattttca tataaataaa
     5161 agaaaatttt ccgctcaatc gcagtaaaca atacctcatc atgcaggctt tcattgaaca
     5221 cttttttcgt tactgcatca atatacaaat gtttgatttg cgtaaaggtg ccataatgat
     5281 gctcaagcat attccgcatt cttaatagca gctctctcgg atcaaagggc tttaccatat
     5341 aatcctcgcc gatctgcaaa ccttgaagct tgctgtccat gtcattgcgg gctgagagaa
     5401 aaataaccgg aatgcccagc cccagctctt tgatttttgc agacatttga tacccgtctt
     5461 cacccggcag catcacatcc ataatgacca aatccacctt ctgaatcacc gaaagcacat
     5521 ccgccccatc tgttttccat atcacacgat atccctcacg ctgcaaaatc ttttgcagca
     5581 aatcaccaat catcacatcg tcttcaacga ttaaaatatg ataacccttc atcctagcga
     5641 acctttctca taaactccat ggacagttta tatatttgat caggctgata taaataaata
     5701 ctgtgtgttc ccctcagttc ctttatatcg gcagtattga attgagccgc aaacgcttca
     5761 tagtcttttc ggttttgtat agcatacttt gaatgtctat tttggtcaga gaccgcatct
     5821 aaaatcaaga cgggagtttc ctttggcgcc ggcagattca cagatttgct gccgttttgg
     5881 taagactgca aaagctcatg ctccatatct gcattaaaaa attgcttaaa gttaatggct
     5941 ttatagattt ctttttcttc atcagttaaa aacgactgcc gaatcacctc aggattatat
     6001 acagcggaag gtataaaccg atgaaaacca atcgaggtta acaggtggaa ccctctcact
     6061 ttcaatcggt caactccgct caatttatac gtgacgtact gctggggcaa tccaatatcc
     6121 atcgcaataa tggccttgat ttccttagga tatttctgta cccaatacat cgcttcaatc
     6181 ccggatatcg aatgaggcat taaaatataa ggaggcttat ttccgctttt cataagcgct
     6241 ttcctcgtct gttccaatac cgtatcaata tctctgtcat cgtgagacac ttcactgtat
     6301 ccataacctg cccgatctac aacagcaatc ttattttctt ttgaaaactt gctgtacagc
     6361 cccttcattt cataagcagg cgcagcaatc cccgaaccgg acataaacac aaacgtatcc
     6421 ttcccgcttc cctcttgata cacattcatc tttttaccat caacttcgac taatgtgcct
     6481 ttttctttaa ggagtgccgc ctccttattt agctggaaat ggtgataaat aaataccgag
     6541 acggatacaa gcaaaaccaa agcagccaag ctgacaaaaa caattttgag gactttccat
     6601 aatgttttca tatgatgctc ctttcacttg ataccgaaag gatacaatat gaaaataaat
     6661 ttctgattaa ctttggaaca gttttatttt cacatttaac tttgctctta cggaaaggtg
     6721 tacatttgga gcatagcaga acatttgatg aatttaccta ctaataaaaa aatatttcat
     6781 taaaaaacct tcctgcttat aggaaggacc gtttcatcat aatattcaac cgtttgtcgc
     6841 acatacaaga tggaaaattt cacaagcctg caatcttttt taacatctta tcccaaccaa
     6901 catctcccat cgttttagta atgatctgac cgtattgact taatagacaa aagctgggga
     6961 tgccttgtac gtgacaagtt gtgacgatct catagttttg gtctaataca atcggaatag
     7021 ctagtcccct atcaagagca tacgtcaaga caacctccat agatttctct tcttctatat
     7081 aggggacatg tactccgatt actttcacat tcatatcctt tatatcttga aggactttgt
     7141 ctgttaattg gtgacagttt ggacagctca ttgaccaaaa atatagaaga agatatgtgt
     7201 cacttggaaa tgtaaaatca ttctgtgatt gattcaacca tggtaaatga cttggccatt
     7261 cagtcaacat ttgttcatct ttcatccttt cagtcctcct tatctttcag ctgaaaagca
     7321 atcagctttc ctaacgcctc ttcagccgca aacggagcaa ctattcctga aggatgttca
     7381 tgctcgtatt ttagacattt atacatctta ggttcaatgc aagacggatc aatccatgca
     7441 atacaccaag atgcccaaaa gcacgattgc ccttcacttt cttgagcgat tttgatgaca
     7501 aagggcagac tgggcttccc gattctcatt aatgaaatcg ccgctgccat tcttgtatct
     7561 tcggcatgac tttttaataa acatgatagc tcctccgctg cttcactcgc gtcttcccca
     7621 aactcaccta aaacagctgc agcaatatta cacaaagagc cttcctcttt taaggcctca
     7681 accaaaactg gtattgcatc ttttcccgat ttctttagct cacctatcgc ttgaacaatt
     7741 gtgatctcat ttgtattcgc taaattctga acagataacg acaatttatc accatttgaa
     7801 ttcataaaag agcccctctc ctcgttttaa aacaataact ctacataacc actatctgaa
     7861 acgataaaac aggcaccaac ttaggctccc tatacagacg accaccacta atgtatacca
     7921 gtcagcagtc aataaatgaa tgacatacaa acaaatgccg ctcaggccta atgccataaa
     7981 aaatgacttg taatattgag gcaatgaaag cttaatatcg tttcggtttg tatgggtaaa
     8041 agcaaaaagc cttattgtca tgaaccatat atatatccct aagccatata tataccaata
     8101 aactaaattc ccaactgcca taaaagtaat agagacacag taaagaatat acaaccagac
     8161 tgtattcgtc agctctagaa taatgatttt tttccaaagg gcaccgggga ataaatgaag
     8221 ggtgcctgat tgagaatgtc tgacaattcc tgaataatag gaacttaaca tgactgccga
     8281 gacgataaga aagaaaagaa gataaaacca ttctggatac atgaaaatga cgacataaaa
     8341 gacgccactg agcatgaccg tatgaatgct atgaaaaaat aaatattttt taaaaagcaa
     8401 cagttccaac cacagaaaag accaaatgcc atttagacct tgcaagcccc accaagtcgt
     8461 ttttggtttc tgtataattt gacctgaacc aaattgacgt tcttgaaatt cctcccagaa
     8521 ttcagcttct ttcaccaacg attccttcat ttctacctga cggctcgtaa aagcaattgt
     8581 cataaaagag actgtgatga tcccaataaa tacaaccaca tacaacggtg acaatgtatc
     8641 aaacactaat ccaacgccac taataaaacg ggcaatgctc tcccatatcc cagatgaatg
     8701 ttggagaaag aagaagtgat aaagacatat ggagtaaatg cctaacatta gaagaaagac
     8761 taaaacgtat acccttttca ttttcttcac tgttctggca caagagaccg acatcaacca
     8821 cacttcagcg ataaaaaaga gccctacaaa aattatggat cggcctaata aattgatatg
     8881 atcaccagac agaagcacaa ttaatacatc accgaatatg gctgagatga tccaaagcaa
     8941 cgccttccaa gtgacgctga aaaataaaac actatacaca acttttgaaa aagatgtggc
     9001 agtgccataa agccatataa tatctgccat ttgacttggt aatcgatttg aaaaaagtcc
     9061 aaatgccgta taaaggataa aaacacctgt aagtacccaa ccagcaatac tcatatccca
     9121 tgtcgtagat ctagaaacaa aacttacttg aaatccgatg gccccaacaa aaaggagcat
     9181 gatcaaaaaa ggcatttttt tttcctgacc ttgactgaca aaacgccttt ttgactgaag
     9241 ccataaaaaa gaaatgatat tattcatctg accattcccc tagaagacga atcacttctt
     9301 ttctccattc cgcttgttct tcttcaaatt gaggaacata caaatcctgc atgagtttgc
     9361 cgtctctaat taatatcacc ctgtctgctg tctctaaaac aaactctaga ctatgagttg
     9421 atacaaatgc tgaacaccct tgatcagttt tagtttttaa ccagttagag agccactgtt
     9481 ttgatgggat atcaaggccc acagtcggtt cgtctaatat atacagcaaa gcgtcttgaa
     9541 caatcgcccc cgccaggaca acccgctgct tttggcctct tgacagctga ccacacaatt
     9601 ggtttgcttt ctcctccaag tgcacttcct tcaatgcatg ataaaccctt tcttttacat
     9661 cttttttacc tggatgatat cctcttgccc taaactgaat atgttcagca gcggttaaag
     9721 cgggatacag atatggctga tcaggaataa gcgcgacttt tttttgaaca tttatatgtc
     9781 ctggaggaga accctcaatg aggatatctc ctttatccgg cttatagaga cctgtcaaac
     9841 atcgaattgt cgttgttttt ccagctccat ttggccccaa tagcgccacg atttctcctc
     9901 gttgaattga gaaagacaaa tgttcaaccc catcaccatt tcgatagcat ttgcttaaat
     9961 tttgtacttg catcaattgc atcaaatctt ctcctaatca ttagagtgaa gtatgctttg
    10021 gctgatcatg atgcatatgt ttctgaagct tgaactgcca cagcggcata cagacgagta
    10081 ctaaattatg aatgaaatga gctactaata cacaaataaa accaaatttg atgaagagaa
    10141 ctcccagaaa gcaaccgaat ataaatagat gcagccaacg tatccacctt gggtcatatc
    10201 cagttacttg gtgcatcacc gcccataaaa aggaagatac aataatggag atgaggatat
    10261 ttccactcat ccaccaaatg actggaatca ttaatagccg atagatgacc tcttctccaa
    10321 ttgaactttg tatgctcata ttcaatcctt gcctaaaggt aaaacctgat agaaagacag
    10381 ttcggttaga aagctcaggt gaaattcgta aataatgctt tttctccaat aagttaaaca
    10441 gcagagaagt cactcctaaa ctaatgagcc ctgctcctat tccatagaaa atctgaacta
    10501 ttggttgttc aaacgttttc catccaaaat caccagcaag cgcatttcct gccaatgaac
    10561 atataaatac tacaaatcct gttaataatc ctgtaagaag tgtagacaaa atacctaata
    10621 aactgaccgt tttgacactc attcttgcat cataagcatt tacaattgtt aattgaatat
    10681 cttctttcag cgtcaatgac tggcatatta taatcaacaa acccagaaca agactgaaga
    10741 ttatgcttgt ttgcacatcc ataaatacaa ggatgagaat cgcaaggatc atcgctaaag
    10801 ctgaacccag cataccagat actccgaatg tcgattccac ttgttcatct cgaatgactt
    10861 tactcatttg atctgttagt atttcttgtt cagtaccaat ataagaaatt cggccgccct
    10921 gtaattcaac agtcaccttg agccgaatat ctcccgcttc cgctatgtac caataagtac
    10981 tgacctcgcg aaaatcctcc tcttttttac cagtaccagt catatttgct ctagaccaaa
    11041 gtccatcagg tgaagccatt aacctctgct tgagtgtccc tggagaaact tgttcgaact
    11101 tctccgaaag ggactttttc ggaacatccg cataaaaaaa agtgatttct cgattggcat
    11161 tgacaccaac tacaaatgat tgattcttgt gaacgaaacg gactctccat gattcgacca
    11221 acccaacatc atataaaacc tttctatttt tcttaagtat gcctaagtga tgaagtttat
    11281 ttactgtatc gtggtcatac caatataccg aataaaaatc ccagttctct acatctatcc
    11341 caacaaactc ttttataaat tgaatgctta tttcttttgc ctcatctttg cttaagagta
    11401 gtgaagcaat tgttgggggt ttgaatttaa taaataacag taatccaaca actgctaaaa
    11461 tactccagaa caccaatgat agactattca ttcctgaatc cctgccttga cttctttttg
    11521 aaaaggcaag tccggttcag atagctcaat tttccctata catttgcaat ttttagcttc
    11581 acattttaac tgaagcaaac gagcaggtga accaatgtca gccatgctat cttctaaaat
    11641 atgccctagc ggcaaatctt ctaattggca aggagttacg tatccatctg ctcttatata
    11701 ccaagcaaga tatcctggtg tacaaaaatc tgttgtgaac gaactctctt cgtgctccca
    11761 atcaattatg ttcaggcgat ctccccattt atgacgtgct tccctaagct gtatttgaac
    11821 catttcttca aaatcaatat ccaaagcctt aaagccttct gtcgcacgtc caacagataa
    11881 cgtttttcct gcacgaaaga taaaagcacc cgcattggca cattgctcca ccacatctga
    11941 tacttcatca gcattggact cattaatggt catagcgaca atcactggaa tgtttgcttc
    12001 tgataatttt ttaatggtat tcatactttc tttaaagccg ccttttctgc ctcttaattg
    12061 gtcatgtgta ttatccatac catcaatact aatctgtaca gaaacattgc ctaagtgact
    12121 aagtaattca acttcttcat ctcgccagtt caatccatta ctaaatacat tcacattggt
    12181 aaacaacgaa cttgcaacaa cgactaattc tttaaaccct ttgatcagct ttgcttcacc
    12241 cccggtcagc gtaatatcag caactccatg ggctgctaac ttttgcataa ccaatatcca
    12301 ttgttcacta gatagttcct caggatatgg tttacctgag ctggcatagc aaaacgaaca
    12361 gcttaaatta caagcatttg tcaactgcaa ggtacaacta ataggtaaat atgaatctag
    12421 cgaacccgaa acgtgtaaag gctgatctaa cagcccttcc gtccaagcct ccgtaaatgg
    12481 atgggcacta agctcttctt ttaattggtc agcactcatc tcttcttttt tcataatctc
    12541 ccaaatacgt gccgttttat gaaggttttt gttttttgat aacagaaacg ccaactgtgc
    12601 tccacgtcca ctgagtctga aataaaacat actttttcta tcaatcatta cagcaccatg
    12661 aggctgtaaa tgaaccgcta actctggcaa atcaaaatct ttaacacgat catatgacat
    12721 ataaaatccc ccttaagaga aaataaggag tataagcaaa tacgctcaac tccctattct
    12781 caaatgttaa atagctctca tagcaggatg cggaagtgca caaacacgtg tcatagcaat
    12841 actcttcgag gcccaacagc ccatgcagcc agcagccttc acaatcgaag tacctcccgg
    12901 cttcataagt ccttttttac taacagattc ccattctttt tggtttcttt tcataagtaa
    12961 acctcctctc aatttttgca tagagtctat tgacatagct cccatgcgat acgataatac
    13021 cagattgcaa ttgtcgaagt aagggggatt ttgtatatta tcctaaaaat tacattaatt
    13081 agtataaata gaatatttta accctttaga aatagaaaag ctgacaagct atcctctcga
    13141 gaaaaacagg agctcacccc taatttttca cttatgtttt gaatattttc ttgttctata
    13201 aacaaaacgt caaaaagacg atccattctg ttataaaaat tctgatgcct aatagattga
    13261 caaacaatct cttcgaggaa aataaatcaa gagattattt tcaaataaac tttagtgatt
    13321 aagaaagcaa tgactttagt taaatgaatg aagtctcctg taatttaaat caaatatcta
    13381 tactaaaaac cccttcggct aaaatatcca aaggggttaa acttcttact ccacagtaac
    13441 actcttcaca aggttacgct gcttatcgac atcacaaccg agatgcaggg cagcattagt
    13501 aagcaatcag ctgcaaccga caacagaaac caacagagta agcgttgggt tactaccaca
    13561 gtacgaatct gttatacgca ttgtctaggc ctttcagcga gatgatgcaa gtgtttactc
    13621 cccaagtggc aacttccttg acgtttacgc ggatgttcag gtttacgttc tcttgtgttt
    13681 ccagtgttcg atcaaggcaa tcgtaccgta cttcagcggc aaagccttaa gaaatccctt
    13741 tcagcttcag tgcgccttcg acacatacag aacgatgtcg gcccttggaa ggctcatgca
    13801 tggactgact ttgaacgctt ataaaaaaca cccgcttgta taacgagcgg atgttagatc
    13861 tttcgaaatt attgattgat atcttttaac gtctgtttcg gttgtgaatg aaaaatcgtc
    13921 aatgatgtaa taattgcaat cataacccct aacgcaccaa cccaggtaat cgaggccaat
    13981 gatacgtttt ccacaaaaac ccctcctata cctgcgccga ccgccatggc gaattgcatc
    14041 attgactgat tcatgctaag caaaacacct gacatttccg gttctattgt agccaggtga
    14101 aattgctgtg tcggaccggt ggaccatgcg gcaaacgacc ataatatgag aatcaccaac
    14161 actccgatat aggaatgagt aacaagtgac agaagaatca gtgtgacgat atgcaacgtc
    14221 atcccgccaa cgagtgtaaa gggcactccc catttatcgg tgctatatcc cccaaatttt
    14281 gatccaacca aactggcaat tccaaatata agcaaaacac cgctaagcag cttgccgttt
    14341 atacctgaga tgttcaagag atacggtgac aaataagtgt aagcaacaga atatcccccg
    14401 agccagaaga aagtgattga taatcccata gccactttcc gttttttgaa aagagcaagc
    14461 tgttgaagca aaggcacagg cttatcccct tcagtatgcg gaagagtaaa gaaaataacg
    14521 accattgcga tcaatcccaa caaagcaatt gctccaaata cagacttcca gcctaatgct
    14581 actgctatca ttcttccaag cggaacacca atgattaaag aagcggtaaa tcccatcacg
    14641 accgtagcga tggcacttcc ctgcttccct tccgatgcaa ttttagcggc aatcgttaag
    14701 gcggtgacaa ccaccacacc tgctcccatc gccataatga tccgcgctgc aataaaccat
    14761 ccataaccag gcagtacaaa agccaggaca ttaccgaaca caaacaaacc tagggcatac
    14821 atcatcaatt tgcgtctatc catacttgct gtcaacgcca taagtacggg tgtagaaaga
    14881 gcatatacaa gtgagaaaat ggtaataagc tggcccgcgg cagctaaagt gatcccgaga
    14941 gtatgagcaa tttgatccaa aattccggaa atgatgtact ctgaggttcc aactaaaaag
    15001 ctgacaatgg ctaaaatata aattttccaa gtgttagaca taaaacattc actccttaat
    15061 tattaataaa catatcacga attatcgata tgttattata aaaaataaac acatcgtaat
    15121 atgtcgatat gtatggtgta aaaataaaca tcttttgatg tttattttta tatctctgtt
    15181 ttaagaaagt cagcaagttt cccaatggct tcttcatctc ttttgtaata tgtgtacttt
    15241 ccaatacgct ccgccttaat caagccggct cttaaaagga tggtaagata ttgagaagct
    15301 gtcgattgcg tcattttcaa tttgtctgtt atttgactga cacatacccc gattgtgttc
    15361 atatcaatcc cttcatgggg tgcaaaatga cgatcgggct ccttcagcca ttgtaaaatt
    15421 tgcagccttg attcatttga taaggctttg aatacttcaa ttggttccat atcgattatt
    15481 atatcgttat attccgatat gtcaatccgt tttttggata tatttaatta aaggtttatc
    15541 tctgactaat tccgtgaaat gaaactgaag acagcatatc aattttgttc accgcttaaa
    15601 tttatcatca agctttacga aattggtctc ataatcatgt cactaaacac ttggaggaac
    15661 ccagcaggtg acaaggatgt tttcaattaa aatgaattta atattgttca agtgactaat
    15721 caccagcatc aaattcatgt atttaaaata gatgtaaaaa aacatccgct cataacaaca
    15781 gcggatgtta gatctttcga caggaataac aagtcagtgt actatgaatt gacatctctt
    15841 tatgtccgct taggttatga ataaaaaata ttacaactta tgaataacca gaatgccttc
    15901 agccctctgc tggcttgaat atcagtgatc ataaaacatt tacattaaag atttttctct
    15961 agatcattac ataatttaaa taccatcact cgttcacctg tccgggagct taaatcagta
    16021 tgaaaacttt tcactttttc tccggttatt tttaagataa tatccttaag gccttctata
    16081 cctgattcga ctaactccga acgagtttgt ttgatagata acataccctc ttttgatcca
    16141 caaactacat attcggctgg tgttaaaatc ccttttaaat tgacgatgat catgtcccgc
    16201 agtatatcag tcttaacaga tacagacccc cgcccaagat aatccttctc ccattgagta
    16261 atggacttac tgatctctga ttcaatagaa cctttcgatt ttttcatatc taatacatct
    16321 cctgagcaaa aaaattgcat attctatttg tggtttcgtc tcattaatat tctcttacaa
    16381 catatccttc atatacacct tcaatatcca aatcagtttt accgtttctt gtgaccacta
    16441 tcattttgga taacatattt tcttccatca tagagtggac atgcaaaaat cgaacatttc
    16501 ctgaatgggt ttcctcaact tcttcataag actttagatg ataaaaatca attgcttttc
    16561 taattagttt tttcatacat tccatatttt cacgatcaaa cgagttcatt tcctctattg
    16621 ttttcgtgac aaaatcagtt aaattagcgc tcatacaatc tccttagaac tgctttctcc
    16681 atatataggt gcgtttagaa gttgaacctg catttcatca tgacaagcaa tgatcaggta
    16741 tggcttgacc tgttttagca tcaggaccct ccataattga gggttttcca tccttatgat
    16801 taacaagtaa acctcgaact ttaacatgtg acggcactag tggatgatgt cgaatcatat
    16861 caacactttt ttcaatggtg tcactgctat tttcatttag ccattcatcg accgtaccac
    16921 ctaaaaattc aggcttgcaa ttttgaaaga gataatccaa tgtttgtatt ttatctttca
    16981 ttgtttcaag ttgagttagt tcattacctg cggaagtctt cttatctttt gttcccacta
    17041 caaaaatctc ctcaacatct tcctggtaaa tcgcaaaaat aacagacctc atgatttctc
    17101 cataaggatg tgagataccg gcaccatagc tttgtatagt caacatattc tctgcagaag
    17161 tgttagtcgc ttcttgtaaa ataggctcca aaccgttttc aatatccgtc aaaaataata
    17221 cttttttatt ttggtttaca ttcatacagc acacctcttc cttatgttta tcatttttgt
    17281 attgagtaac aaatccattt gataaatgct tagattcatc attaccagct ttcccaacat
    17341 ccttctggat caagactagc cagtcggacc catccatttt gaactttttg cagaaatgct
    17401 gagtcatgat ttaataaccg ttctacatat tctctaggtg cttggatgag tatcaataaa
    17461 cgaagaggag aatgataggc ctcgtgatct gattccatga cggattgcca aggcagtccg
    17521 gctaacaagt cgcttgcatt cccctgcata acgccgagac ctgcagtaac ggtttgagtt
    17581 gctttatttc cgctgccata ataatgaggt gctaccgttg atgcgtaata ttgtaaatta
    17641 atccattggg ctacagtacc aggtcctgca ataatatttg ctaggagttc accgctttca
    17701 tcctgcttcc aatcataatt atggagaaag gctctgcctt ccaaatcaca atcctgagtt
    17761 agttcacgtt taccgataat aaaagcggca ttacgagcca gtccccattc cggacgtatc
    17821 tcactccaat cttccgcaaa tcggtttgcc tcagccttcg gattttttag cttcgattgg
    17881 aaattcggta attggtccag acgttctgcg tttacatgat gtctcacttt cggcataaca
    17941 gcttcaattt gttcaaatgc ttcttgtgca gcttcggaaa gctctggtac ataaagccaa
    18001 tgtaattcat ctactgttgt attatgttcg gctgctgcaa aaaccgtatc ctcaggaatt
    18061 tgaattcctt cagctaaaag agcttctcgt acctctgata gattacataa agcagctaaa
    18121 actctagcat tgaatccacc ggccgctcca ccgcaggcac cacaatcaag ggctgcggaa
    18181 taaggattat tagtgctttg actgccatgc ccgcagatca cgaccaaggg tgcgatgttt
    18241 tctgttaacc ccatcatttt taatgcttgg cgagcatagt tcactttttc ttcatcagta
    18301 aaacccacag gtatttctgc ctctgtagta tcatgatgaa gcgagagttt tgtatcaggt
    18361 ttgcgcaacc aagcctcacg gagattacga atgaaacgac ccgcctttct tggcacaaag
    18421 ctacgggctg ccatttgcag agtaagccaa ggtccgctta gctcaggcag tagtaagctc
    18481 gaaagcgcat tctgcttcat cgttttaaag gtatggctta aagaatgaat tgcttgcttc
    18541 cgttgattgt attttttgaa tacatcctca tctgcgaact cttttatttt attttgcggt
    18601 ttttgtatga tcggtaagga agcatgactg tgtttactgc caagttcaca agttgcaatc
    18661 ggcaccccga agaaaccagc aataccaatc gtttcaaacg ggccttcttt ttctagttga
    18721 cgacgaaaag gttctgatcg tacatcgatg cagaatgcta attgagctaa tgcagatttt
    18781 ttaccgccgg tttcacgttg tttcgagatg atcttctggc ttaatcgatc agtgtacgtt
    18841 tgctcccaag cttctaacca aagtttcttg cgaagtttct catcaaagct gtaggcaaat
    18901 gataaatatt cgttttgttc aatggccgtc atctgtgacc attcttctaa tgtaagccca
    18961 ccccaatgaa tccaagctgc tataagggga gcaattgaaa tttttttctt agatcgttca
    19021 ttagtcaaag gtaagtatgg ctttataaga gcccactcca tggatattcg aaccgctaaa
    19081 tattctgtta ggagtgcatg ttcgtggctc gattgctggg agcgccaaag catcatccct
    19141 gcccatccag gtaatgaaag taaatgacct tcaagataag tctggatttc tgactctggg
    19201 atttctagtg cgaataaggc ttcctgtaaa gccaaatgtg cctctttcgg ccaatccttt
    19261 accctttctc gctgcttttt gctaagtgct ggatcatatt gaatgagatg ctgccaagca
    19321 cgatagaaac cttcctcacg attaggcatt gtccagcctg cttgagagtc atcaagatat
    19381 aatttactcc acttaatgac atgatgatct agtatattga ctagcctttc accatcttga
    19441 ttctctatat acgaacttag tggctgcata aacacattct cgatatgatc caatccacta
    19501 cattcaccta ctaatttctc cagctcatgt gatgataaaa ggtcggaagg caggggatct
    19561 aactttaatg cagcatgaca aaatcgctct gctacgtccc gtgggatgtg ataggaatgt
    19621 gaatcaagcc aacgctgcag ccccatttcc acaaaatctt catcaatctc acctttattc
    19681 tttgctgaac ggatcataga cgcactaggg tatatatcaa catcgcgagt gtttttcaac
    19741 caacttgcaa cctgatcaaa agattggttt tcaagtccca tccacggatt acgtgcagca
    19801 aatgtagaaa taggccaaag tggcgcaatc actcggctgg cagatgtaat gaaaacgtta
    19861 agatcccttt cttgaacatc aatatctgta tctatctttt ttacattatc tttcgttaat
    19921 acggatgtaa tgcccatctc agattagctc cttatgaaat atattgttta aggtagtctg
    19981 ggtgactctc tactgacttt ggttttgctt cacctaatcg gactaaccaa agatagagta
    20041 ccgcgaaaaa aacggaagaa cgatgacgag caacccacgt acctaaagca ctgccaaaga
    20101 gtaagagaca tacgacaatc atgacagctg acattggagg ttgaacactt tgaaaaatgg
    20161 ttgtatgcag ccacttatag aaaaggttat gaatgataaa atatacgagg gctgctcctc
    20221 ctaaaacagt taagccggca attcggccaa ttcggccctc tccaaaagcg acaagctgag
    20281 tccaagaaac ggacaatgac caccctaaga tcagcgcact aatgaattga tatccctctc
    20341 caggagcagc gagccaaaaa gcaacgccta taactaagga taaaatccga ccagccatga
    20401 cccataaata ggatgttctt tcattagtac gagccgatac ttcatgacgc cctactgctg
    20461 aaccagcttg tagaaacaac gtagccttga ataaaccatg taaaataaga tgaataatgg
    20521 ctgctatata ggcgcctaat gcacactgaa tgagcataaa tcccatttgt ccaatcgtgg
    20581 agcctactaa ttggcgttta taatcaactt ggactaaact aataccagtt ccaatcaata
    20641 cagaaatgcc agcaagcaga agtaagacaa tcgaagcgat accaccatga aatagaggtg
    20701 aaaatcgagt tagtattatt cctcctgcat ttactaaacc tgcatgcata atcgcagaaa
    20761 caggggttgg agcaacaata gactcaacca accatctttg gaatggccat tgagctgcag
    20821 gaatgatcac cgccagtaca atcaatagct gaatccctgt tctctcccat tctcccagcc
    20881 cagctaaact ttcatttgtt acaaccaatg ataactgcca ttgacctgtg gcatgaaaaa
    20941 gccacatcat cgcaaaaaac aaagaaaacc aacttaacaa aaataagcgg ccagaaacct
    21001 tggctgcttc actagccact tgccaagcac tgtttaacct aataagtaag gttaaaccaa
    21061 caagagttgc tccccaaaat agaaccatca agcggagatc gccacttaac catgtcattg
    21121 aagcagcacc tgttgtgaaa gtaaaaagcg taaaatattt tcgataagat cgatccccca
    21181 gtaagtaacg tacagaaaaa cgctgaatga tgaatccaat tgcaagaaca aagaaagtca
    21241 ttaaacaagc taaggaatct aagtgccaag ggccaacatt tccactcaca ccactattgg
    21301 caagaatcag taaggaaacc aaaagtggta aagccaagat cccaatatga atgcgaacaa
    21361 aactcaaggg tactcgcgga tgtaaaaaca atagtccact tatcccagaa gccataagca
    21421 taataaaaaa taatgttaat aatgatgaca aactcagcga aactaacatt ctaaattctc
    21481 ccttttttaa aactcattat tgttcaaaag acctttcaat cagatcatat ttgtctatta
    21541 tttatttcaa tttttattta tctctatttt tttccaacaa aaaagccgac aacttcaaaa
    21601 tttcaatgat tatgttagat cattgaatcc tgaaaattgt cggcttcact tcaactaact
    21661 tcagcgaagt tttttgaagt gctaccttat tattggtctt tactttatgc tatgttttca
    21721 tttcctttaa tatagtcctg attatcaaat aaatcgactc atgttctctt ccttttttat
    21781 taggatactt tttcatcctg tcaatattta aaattaaata tgaaaagaat gctaaaggcc
    21841 tacatatttt aaaaggaaaa ataaacatct gcatcgatta ttaagttacg tataagatat
    21901 gctaaatttt aaaagttgtc aagtttatca gtaaactggt ctttactttc ctctaaaata
    21961 atccaaccca ttatagatga ttcactttca atgaggaaca tccatttcag atgaagctcg
    22021 cttttccaga tcgaggagca gtgtcttcat ttcgaatccg ccccgatagc cagttaatga
    22081 gccattcttt cctattacac gatggcacgg tacagtaatt aataccggat tagccccaat
    22141 agccgcacct acagcacgaa cagctgctgg tttatttatt tcatttgcaa tgtcggaata
    22201 ggatttcgtc tgtccataag gaatttcaca aagtgcattc cagactgcta gctgaaattg
    22261 cgtaccggcg tactcaactg gaacagtaaa gtttttccgc tttccttcta gatattgagt
    22321 gatttcaacg gcatagggtt caagcttatc atcatcttca acaagaagac ttcccggaaa
    22381 gcgtttccta gcccattcga acaattcctc gattggtttg ttctgtgaac ctacaaacac
    22441 aagccccttt aaagttgaag caatgtaaaa attccaatcc ttgaacatta gtaaagacca
    22501 ataaagggtt ggtttattct ttgtttccat tgtatgtttc ctccatttta ctcatttgac
    22561 gaaattgtgc tggtgtctgt ccagttttct ttttaaataa agtaataaaa taaggcgcgt
    22621 tagctatacc cacacatata gcgatatctc caatcgattt atttgtctga atcaaatact
    22681 ttttagccgc gtgtactcta acttgttgta tatactcaac cagcgtaatg cctttaatct
    22741 ttttaaatgt tcgatgcata tgatacggac tcccatgaca aatatctgct aacgattcta
    22801 gcgttaattt ttctgtgaaa tttttatcaa tgtattcagt aattaaatca acccactcgc
    22861 tatcaggcat tttttcatta gtgggcttgc aacgtttaca agggcgaaaa tttgcgcgga
    22921 gagcttcttc tgcgtttgga aaaatacata catt
//
