LOCUS       NZ_SHKU01000009        10405 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. BK022 Ga0307664_109, whole genome shotgun sequence.
ACCESSION   NZ_SHKU01000009
VERSION     NZ_SHKU01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. BK022
  ORGANISM  Streptomyces sp. BK022
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 138648)
  AUTHORS   Kalluri,U.
  TITLE     Understanding the reciprocal impacts of modified plant cell wall and
            associated microbiome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 138648)
  AUTHORS   Kalluri,U., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 215.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004217355.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 02:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,482
            CDSs (total)                      :: 6,401
            Genes (coding)                    :: 6,280
            CDSs (with protein)               :: 6,280
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 49 of 121
            Pseudo Genes (incomplete)         :: 92 of 121
            Pseudo Genes (internal stop)      :: 16 of 121
            Pseudo Genes (multiple problems)  :: 29 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:05:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 77875
            Orig. end                         :: 88280
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SHKU01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    132..146
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     misc_feature    complement(246..260)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.0"
                     /tool="antismash"
     gene            complement(623..1465)
                     /locus_tag="EV284_RS31460"
                     /old_locus_tag="EV284_6293"
     CDS             complement(623..1465)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007497778.1"
                     /locus_tag="EV284_RS31460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6293"
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="WP_130340230.1"
                     /transl_table=11
                     /translation="MKTLLRRRPRVLAAITATAAIALVAGCSSSGGGGGKTTVKGVHLA
                     NAGALTVCTHLPYPPFQSVIDGKVQGFDVSLIDLVAKDLGVKQKIVDTPFENFKTGAFL
                     NSGQCDLAAAGMTITDERKKNVDFSDPYFEATQAVLADKKSGIKSLADLKGKKLGAQAQ
                     TTGEDLAKSKGLDPVSFHSSDAVLNGLRTGQVQAVVIDYPVVQGWLKDKANASAFQVVE
                     NINTGEQYGFTVKKGNTALRTAINKALAHAKSDGEYKKIYEKWIGPYDPSAASPSAGS"
     gene            1668..2768
                     /locus_tag="EV284_RS31465"
                     /old_locus_tag="EV284_6294"
     CDS             1668..2768
                     /NRPS_PKS="Domain: Aminotran_5 (16-311). E-value: 1.3e-29.
                     Score: 95.2. Matches aSDomain:
                     nrpspksdomains_EV284_RS31465_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="EV284_RS31465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6294"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_130340232.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.6e-25, bitscore:
                     80.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLPPLTAARFAAIEDRVARLLDTRQDVLVTQGEALLPLE
                     GAIRAAAGPGTVALNVITGPYGQTFGDWLRDSGATVHDLAVPFDSAVTADQVRAAFAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEAVREQGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWERMAANPDAPRHSYLSLLDWKERWIDGGRKALPHAP
                     AQLEMLALDACMERIEADGLDTVMARHRDLARATRAGALALGGGLEPYVREERDAAPVA
                     TTLRVPAGTVASELVARALESDPGLPLAAGGGALAKEMIRVNHYGPDATPQVVRRALVA
                     LGTALAGQGLAVDPEAAVAAVEAFAL"
     aSDomain        1716..2600
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EV284_RS31465_Aminotran_5.1"
                     /evalue="1.30E-29"
                     /label="EV284_RS31465_Aminotran_5.1"
                     /locus_tag="EV284_RS31465"
                     /protein_end="311"
                     /protein_start="16"
                     /score="95.2"
                     /tool="antismash"
                     /translation="AAIEDRVARLLDTRQDVLVTQGEALLPLEGAIRAAAGPGTVALNV
                     ITGPYGQTFGDWLRDSGATVHDLAVPFDSAVTADQVRAAFAEHPEIDFVSLVHAEAATG
                     NTNPVAEIGEAVREQGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSAI
                     SVSERAWERMAANPDAPRHSYLSLLDWKERWIDGGRKALPHAPAQLEMLALDACMERIE
                     ADGLDTVMARHRDLARATRAGALALGGGLEPYVREERDAAPVATTLRVPAGTVASELVA
                     RALESDPGLPLAAG"
     misc_feature    2952..2967
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2989..3002)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     misc_feature    3049..3061
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.63"
                     /tool="antismash"
     gene            3086..3604
                     /gene="ectA"
                     /locus_tag="EV284_RS31470"
                     /old_locus_tag="EV284_6295"
     CDS             3086..3604
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545812.1"
                     /locus_tag="EV284_RS31470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6295"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_130340234.1"
                     /transl_table=11
                     /translation="MTSASADLLIDRPAVTDGAALWRLARDSKTLDLNSSYSYLLWCRD
                     FAGTSAVARGADGEPVGFVTGYVRPDRPHTLLVWQVAVDGSQRGRGIAAALLDGLTARL
                     ADEQGVTDVETTITPGNTASERLFTSFAERHGAGLTREVLFPRELFPDGPHDPEVLYRI
                     GPLTHVPAH"
     gene            3628..4899
                     /gene="ectB"
                     /locus_tag="EV284_RS31475"
                     /old_locus_tag="EV284_6296"
     CDS             3628..4899
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 1.7e-72.
                     Score: 236.2. Matches aSDomain:
                     nrpspksdomains_EV284_RS31475_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="EV284_RS31475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6296"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_130340236.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.3e-78, bitscore:
                     255.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPVVFDQARGSRMYDEDGHAY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLDRDGVTHGLDMSTTAKRTFLQTFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALKELCERQDMLLIVDDIQMGCGRTGPFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALETYWADGAAMEKQTRTRGEQVEREL
                     ISITEENLADVKEYRGRGLVWGLEFHHGDRASRVAKRAFELGLLIETSGPQGEVVKLLP
                     ALTITPEELDEGLSILARAVRETA"
     aSDomain        3715..4725
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EV284_RS31475_Aminotran_3.1"
                     /evalue="1.70E-72"
                     /label="EV284_RS31475_Aminotran_3.1"
                     /locus_tag="EV284_RS31475"
                     /protein_end="366"
                     /protein_start="29"
                     /score="236.2"
                     /tool="antismash"
                     /translation="DQARGSRMYDEDGHAYLDFFAGAGSLNYGHNNPVLKRALIDYLDR
                     DGVTHGLDMSTTAKRTFLQTFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKGR
                     EAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFDGTVPDFLWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALKELCERQDMLLIVDDIQMGCGRT
                     GPFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFVT
                     ATAALETYWADGAAMEKQTRTRGEQVERELISITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="EV284_RS31480"
                     /old_locus_tag="EV284_6297"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="EV284_RS31480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6297"
                     /product="ectoine synthase"
                     /protein_id="WP_130340238.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.1e-55, bitscore:
                     180.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFRDIEGTDRHVKAKSGTWESKRIVLAKERVGFSVHETVLY
                     AGTETSMWYANHIEAVVCTEGEAELTDHEDGQVYTITPGTMYLLNGHERHTLRVKEDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5411..6310
                     /gene="thpD"
                     /locus_tag="EV284_RS31485"
                     /old_locus_tag="EV284_6298"
     CDS             5411..6310
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="EV284_RS31485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6298"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_130340240.1"
                     /sec_met_domain="PhyH (E-value: 7.5e-41, bitscore: 133.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLTTTPTDLYPSRGATEVRVPRQDPVVWGSPDTPGPIRAAELE
                     GFERDGFLTVDQLITPAEVQVYRRELDRLVGDPAIRADERSIVEPQSGEIRSVFEVHRL
                     SELFANLVHDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     HMRTVSVSIALTENHDTNGGLMIMPGSHKTYLGCAGATPKDNYKKSLQMQDAGTPSDEA
                     LTEMAGEHGIRLFTGAAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFA
                     APVRRPEFIGARDFTPVR"
     gene            complement(6388..7449)
                     /locus_tag="EV284_RS31490"
                     /old_locus_tag="EV284_6299"
     CDS             complement(6388..7449)
                     /NRPS_PKS="Domain: Aminotran_5 (46-347). E-value: 1.3e-22.
                     Score: 72.1. Matches aSDomain:
                     nrpspksdomains_EV284_RS31490_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671936.1"
                     /locus_tag="EV284_RS31490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6299"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_130340242.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.3e-18, bitscore:
                     59.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METTETFESLVRAEFAPVGTFLNTASNGLLPARAVTALHESALLR
                     AQGQPLTALWDEVEVCRAAYARLAGVPVERVALGASVAAHSAVIAASLPSGAEVLTAED
                     DFASVPTPFHVRGDLKVRAVPVERIAESVRPGTALVAVSAAQSADGKVADLPALREAAA
                     GHGARTYVDFSQAAGWLPMEAGEWDFTASVSYKWLLGPHGVAFLVLPEDFGGLTPLLAS
                     WVAGERPWDSCYGPVAELAHSARRFDLSPALFCYPALRRSLELIEELGVPEIHAHDTAL
                     ADRYRAGLAALGHEALPAPGSAIVSVPGLGSRQPELGRAGIEVSDRAGNLRASFHLYNT
                     EADVDRLLDALAG"
     aSDomain        complement(6409..7311)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EV284_RS31490_Aminotran_5.1"
                     /evalue="1.30E-22"
                     /label="EV284_RS31490_Aminotran_5.1"
                     /locus_tag="EV284_RS31490"
                     /protein_end="347"
                     /protein_start="46"
                     /score="72.1"
                     /tool="antismash"
                     /translation="QGQPLTALWDEVEVCRAAYARLAGVPVERVALGASVAAHSAVIAA
                     SLPSGAEVLTAEDDFASVPTPFHVRGDLKVRAVPVERIAESVRPGTALVAVSAAQSADG
                     KVADLPALREAAAGHGARTYVDFSQAAGWLPMEAGEWDFTASVSYKWLLGPHGVAFLVL
                     PEDFGGLTPLLASWVAGERPWDSCYGPVAELAHSARRFDLSPALFCYPALRRSLELIEE
                     LGVPEIHAHDTALADRYRAGLAALGHEALPAPGSAIVSVPGLGSRQPELGRAGIEVSDR
                     AGNLRASFHLYNTEADVDRL"
     gene            complement(7526..8245)
                     /locus_tag="EV284_RS31495"
                     /old_locus_tag="EV284_6300"
     CDS             complement(7526..8245)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381151.1"
                     /locus_tag="EV284_RS31495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6300"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_130340244.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDEVEVVHRSFELDP
                     GRAKGDVQPVLDMLTRKYGMSAAQAEAGEDNLGAQAAAEGLDYRTRGRDHGSTFDLHRL
                     LHFAKEQGRQEALLDRLYRANFADEQSVFTGGDDHLVELAAEAGLDADATRAVLADPTA
                     YADEVRADEREAAQLGANGVPFFVLDRTYGVSGAQPAEVFTQALAQAWGDRSPLRTVTG
                     DDAGTCGPDGCAVPQQG"
     gene            complement(8320..8823)
                     /locus_tag="EV284_RS31500"
                     /old_locus_tag="EV284_6301"
     CDS             complement(8320..8823)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067468.1"
                     /locus_tag="EV284_RS31500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6301"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_207229184.1"
                     /transl_table=11
                     /translation="MISDETADALVIRPAEPGEAEIIAALHARARATYYPDGLPPSDFD
                     WTDAWRGSIERPDGQVLCGVRDGEIVAVASFRTPEGEPVDTVKLYQFHVSPDHWRAGLG
                     AALHAACVERWLADRVRAAVLDVHVDNRRAQAFYARQGWVPDPAKPPAEDDHHLFLRWT
                     PPGE"
     misc_feature    complement(8817..8831)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 20.16"
                     /tool="antismash"
     gene            complement(8863..9471)
                     /locus_tag="EV284_RS31505"
                     /old_locus_tag="EV284_6302"
     CDS             complement(8863..9471)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="EV284_RS31505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_6302"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_130340246.1"
                     /transl_table=11
                     /translation="MDIELPELPFPLRTYGPDGHWSYEKGVLTGWAGPRQDRFVTPTGE
                     ALDPAADAPRLLGSPEGDFQLIARVTVGFNAAFDAGVLYVHVGDRNWAKLCLENSPDVP
                     TICTVVTRGHSDDANSFTVPGSSVWLRVSRTGRALAFHASRDGERWTFVRLFTLGDEKE
                     TGAALIGFMTQSPLGEGCVVTYDHLEFRPTWPADLRDGS"
ORIGIN
        1 gttggtgagc ggcggcagga tgatgcggaa cgcctgcggg atgacgatgg agatcatggc
       61 ccgcgcgggc gagaagccca gcgaacgcgc cgcctccatc tgccccttgg gcaccgcctg
      121 gatgcccgcc cggatcgtct cggccatgta ggcggccgag accaggccga gggcgacggc
      181 gaccttgccg taggtgccgc ccggatactg cacgtccggg aaggccagcg gcacgccgac
      241 gccgacgaag atgaagatca gcagggcggg caggccccgg aagatctcga tgtagatgcc
      301 ggcgagccag cggtacggcc ccacggagga cagccgcatc agggcgacga cgacgcccag
      361 cacgagtccg accacgaagc cggacagcgt gtagagcacg gtgttcttca gcgccagcgt
      421 gatgatgtcc gggaacatct gctcggcgat gggcagttgc gcgaactggt tcttcagccg
      481 tccccagtcc gcggagaacg cgaaggcgag gacggcggcg acgaagacga cgtactgcgc
      541 gccgcgtgag agcctgcgcc gctgcctgcg ggtcaggccc ttcttgcggg gctggatgcg
      601 ggtgtccgtg tcggccatcg ggtcaggagc ccgcggaggg ggaggcggcg gacgggtcgt
      661 aggggccgat ccacttctcg tagatcttct tgtactcgcc gtcggacttg gcgtgggcca
      721 gcgccttgtt gatcgcggtg cgcagggcgg tgttgccctt cttcaccgtg aagccgtact
      781 gctcgccggt gttgatgttc tccaccacct ggaaggcgga ggcgttggcc ttgtccttca
      841 gccagccctg gaccaccggg tagtcgatga ccacggcctg gacctgcccg gtgcgcaggc
      901 cgttgaggac cgcgtcggag gagtggaagg agaccgggtc gagacccttg ctcttggcga
      961 ggtcctcacc ggtggtctgc gcctgcgcgc cgagcttctt gcccttgagg tccgcgagcg
     1021 acttgatgcc gctcttcttg tcggccagca cggcctgggt ggcctcgaag taggggtcgg
     1081 agaagtcgac gttcttcttg cgctcgtcgg tgatcgtcat gccggccgcg gcgaggtcgc
     1141 actggccgga gttgaggaag gcgccggtct tgaagttctc gaacggcgtg tccacgatct
     1201 tctgcttcac ccccagatcc ttggcgacca ggtcgatcag ggagacgtcg aagccctgca
     1261 ccttgccgtc gatcaccgac tggaacggcg ggtagggcag gtgggtgcag acggtcagcg
     1321 ccccggcgtt ggcgaggtgg acccccttga cggtggtctt cccgcccccg ccgcccgagg
     1381 aggagcagcc ggcgacgagc gcgatcgcgg ccgtcgcggt gatggctgcc agcacacgcg
     1441 gccggcgccg aagaagggtc ttcatggggg gaatctcccg atcgggggaa ctgtgggttc
     1501 cgattataag gagaggtttg ggcctctcaa atcaaactga tggcttcgcg gccgtccagc
     1561 ctaagatgtc gccggtcaaa acggcacgga ggccgccagt taccctcgtc accgtcggcc
     1621 cgccggggcc ggccccgtgg acctcgtgag cgaagaaggc acccgccgtg actcatcccc
     1681 tcctcgacct gcccccgctg accgccgcgc ggttcgccgc catcgaggac cgggtggccc
     1741 ggctgctgga cacccggcag gacgtgctgg tcacccaggg cgaggcgctg ctgccactgg
     1801 agggcgcgat ccgcgcggcc gccggtccgg gcaccgtggc gctgaacgtg atcaccggcc
     1861 cgtacggcca gaccttcggg gactggctgc gcgactccgg cgccaccgtg cacgacctgg
     1921 cggtgccctt cgactcggcg gtcaccgcgg accaggtccg ggcggcgttc gccgagcacc
     1981 cggagatcga cttcgtctcc ctggtccacg cggaggcggc caccggcaac accaacccgg
     2041 tcgcggagat cggcgaggcg gtgcgggagc agggcgccct cttctacctg gacgcggtcg
     2101 cctcggtcgg cgccgagccg gtgctgccgg acgcctgggg cgtggacctg tgtgtgatcg
     2161 gcgcccagaa ggcgatgggc ggccccgccg gggtctcggc gatctcggtg agcgagcggg
     2221 cctgggagcg gatggccgcc aaccccgacg cgccgcgcca ctcctacctc tccctgctgg
     2281 actggaagga gcgctggatc gacggcggcc gcaaggcgct gccgcacgcc ccggcgcagc
     2341 tggagatgct ggcgctggac gcctgcatgg agcggatcga ggcggacggc ctggacacgg
     2401 tgatggcccg gcaccgcgac ctcgcgcggg ccacgcgtgc gggcgcgctc gccctgggcg
     2461 gcgggctcga gccgtatgtg cgcgaggagc gggacgcggc gccggtggcg accacgctgc
     2521 gggtgccggc gggcacggtg gcctcggagc tggtcgcgcg ggcactggag agcgacccgg
     2581 ggctgccgct ggccgccggt ggcggggcgc tggccaagga gatgatccgg gtgaaccact
     2641 acgggcccga cgccacgccg caggtggtgc gccgcgcgct ggtggcgctg ggcacggcac
     2701 tggccggaca ggggctggcg gtggacccgg aggcggccgt ggcagcggtc gaggcgttcg
     2761 cgctctgacc tacggcagcg acgagcggcc cgcgcgatcc cgcgcgggcc gttttccttt
     2821 ccggccccca cttcagccgc ttatccatat gacgtaaatt ccccttgaat tcagtcgaag
     2881 aagctcagcc cttttctcct gcccggtttt tgcagagcta aacaacttcg atttccaacg
     2941 ggtttccatt ctgtgacaca ctccacacgg ggggcttttt gtcccgcttt gacctaggtc
     3001 aaagccgaca taccgccgag cggtccgggc cctaccgaga tcacccccgt gcatttcgca
     3061 attcacctcg ctaaattcag gccgcatgac ttccgcatcc gccgacctgc tcatcgacag
     3121 acccgcggtc accgacggcg ccgcactctg gcgtctcgcc agggactcga aaacgctgga
     3181 cctgaactcc tcgtacagct atctgctgtg gtgccgtgac ttcgccggca cctcggcggt
     3241 ggcccgtggg gcggacgggg agccggtcgg gttcgtcacc ggctacgtcc gcccggaccg
     3301 cccgcacacg ctgctcgtct ggcaggtggc cgtggacggc tcccagcggg gcaggggcat
     3361 cgccgccgcg ctgctggacg ggctgaccgc gcggctcgcc gacgagcagg gcgtgacgga
     3421 tgtggagacg accatcactc ccggcaacac cgcctccgag cggctgttca cctcgttcgc
     3481 cgagcgccac ggcgccgggc tgacccgcga ggtgctgttt ccccgggagc tgttccccga
     3541 cggcccgcac gaccccgaag tgctgtaccg cataggcccg ctgacccacg tcccggcgca
     3601 ctgacgtccc ccgaggagcg aatcaccgtg accatcaccc agcccgacct gagcgtcttc
     3661 gagaccgtgg agtccgaggt gcgcagctac tgccgcggct ggcccgtcgt gttcgaccag
     3721 gcgcggggca gcaggatgta cgacgaggac ggccacgcct acctcgactt cttcgccggg
     3781 gccggctcac tcaactacgg ccacaacaac cccgtgctga aacgggcgct gatcgactat
     3841 ctggaccgcg acggggtgac ccacggtctg gacatgtcga ccacggccaa gcggaccttc
     3901 ctccagacct tccaggacct ggtgctgcgg ccgcgcgacc tgccgtacaa ggtcatgttc
     3961 ccgggcccga cggggaccaa cgcggtggag tccgcgctga agctggcccg gaaggtgaag
     4021 ggccgcgagg cgatcgtgtc cttcaccaac gccttccacg gcatgtcgct gggctcgctc
     4081 gcggtcaccg gcaacgcctt caagcgggcc ggcgccggcg tcccgctggt gcacggcacc
     4141 ccgatgccct tcgacaacta cttcgacggc accgtgccgg acttcctgtg gttcgagcgg
     4201 ctgctggagg accagggctc cggcctgaac aagcccgccg cggtgatcgt ggagaccgtg
     4261 cagggcgagg gcggcatcaa cgtggcccgg cccgagtggc tgcgcgcgct gaaggagctg
     4321 tgcgagcggc aggacatgct gctgatcgtc gacgacatcc agatgggctg cggccgtacc
     4381 ggtcccttct tctccttcga ggaggcgggc atcacgccgg acatcgtcac ggtctccaag
     4441 tcgatctccg gatacggcct gccgatgtcg ctgtgcctgt tcaagccgga gctggacatc
     4501 tgggagccgg gcgagcacaa cggcaccttc cgcggcaaca acccggcgtt cgtcaccgcc
     4561 acggccgcgc tggagacgta ctgggccgac ggcgccgcga tggagaagca gacccgcacg
     4621 cgcggcgagc aggtcgagcg cgagctgatc tccatcaccg aggagaacct ggccgacgtg
     4681 aaggagtacc gcgggcgcgg gctggtgtgg ggcctggagt tccaccacgg cgaccgagcc
     4741 tcgcgggtcg ccaagcgcgc cttcgagctg gggctgctca tcgagacgtc cggtccgcag
     4801 ggcgaggtcg tcaagctgct gcccgcgctg accatcaccc cggaggagct ggacgagggg
     4861 ctgagcatcc tcgcccgcgc cgtccgcgag accgcctgac actacgaggg ccagaccgcc
     4921 tgacccgaca gaccgcctga ccagctagac cgcctgacca gccagaccgt cttaccggct
     4981 ggtgaagagg aggagacacc gtgatcgtcc gttcgttcag ggacatcgag ggcaccgacc
     5041 ggcatgtgaa ggccaagtcg ggtacctggg agagcaagcg catcgtgctc gccaaggagc
     5101 gggtcggctt ctccgtgcac gagacggtcc tgtacgccgg gaccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtcgtctgca ccgagggcga ggccgagctg accgaccacg
     5221 aggacgggca ggtgtacacc atcacgcccg gcaccatgta cctgctgaac gggcacgagc
     5281 gccacacgct gcgggtgaag gaggacttcc gctgcatctg cgtgttcaac ccgcccgtga
     5341 ccggccggga ggaccacgac gagaacggcg tctacccgct gctcaccgag cccgaggagg
     5401 tgtgaggacc atgaccacgc tcaccaccac ccccaccgac ctctacccga gccggggcgc
     5461 gaccgaggtc cgcgttccgc gccaggaccc ggtcgtctgg ggttccccgg acacgccggg
     5521 cccgatccgg gcggccgagc tggaggggtt cgagcgcgac ggcttcctca ccgtcgacca
     5581 gctcatcacc cccgccgagg tgcaggtcta ccggcgcgag ctggaccggc tggtcggcga
     5641 cccggcgatc cgcgccgacg agcgctcgat cgtggagccg cagtccgggg agatccgttc
     5701 cgtcttcgag gtgcaccggc tcagtgagct gttcgccaac ctcgtgcacg acgagcgggt
     5761 ggtcggccgc gcccggcaga tcctcggctc ggacgtctac gtccaccagt cgcggatcaa
     5821 cgtcaagccc ggtttcggtg ccagcggctt ctactggcac tcggacttcg agacctggca
     5881 cgccgaggac ggcctgcccc acatgcgtac ggtgtccgtc tcgatcgcgc tgaccgagaa
     5941 ccacgacacc aacggcggcc tcatgatcat gccggggtcg cacaagacct acctgggctg
     6001 cgcgggcgcc accccgaagg acaactacaa gaagtcgctc cagatgcagg acgcgggcac
     6061 cccatccgac gaggcgctga ccgagatggc cggcgagcac ggcatccgcc tgttcaccgg
     6121 cgcggcgggc tcggccacct ggttcgactg caactgcatg cacggttccg gcgacaacat
     6181 cacgccgttc ccgcgcagca acgtcttcat cgtgttcaac agcgtggaga acacggcggt
     6241 ggagccgttc gcggcgccgg tgcgccggcc ggagttcatc ggggcgcggg acttcactcc
     6301 ggtgcggtag cccccgcccg accccctgtc accgggccgg tgcctcttcg tgtgggacgg
     6361 gaggcaccgg cccggtcatg tccgggctca gccggccagc gcgtccagca ggcggtcgac
     6421 gtccgcctcg gtgttgtaga ggtggaagga cgcccgcaga ttgcccgccc ggtcggacac
     6481 ctcgatgccg gcgcggccca gctccggctg gcgggagccg aggcccggca ccgagacgat
     6541 cgcggagccc ggggcgggca gcgcctcgtg gcccagcgcg gcgagcccgg cccggtagcg
     6601 gtcggccagc gccgtgtcgt gcgcgtggat ctcgggcacc cccagctcct cgatcagctc
     6661 cagggagcgg cgcagcgcgg ggtagcagaa cagggcgggg ctgaggtcga accgccgtgc
     6721 ggaatgggcg agttcggcga cggggccgta gcagctgtcc cacggccgct cgcccgccac
     6781 ccagctggcc agcagcgggg tgaggccgcc gaagtcctcc gggaggacga ggaaggcgac
     6841 gccgtgcggg ccgagcagcc acttgtagga caccgacgcg gtgaagtccc actcccccgc
     6901 ctccatcggc agccacccgg ccgcctggga gaagtcgacg taggtacgcg cgccgtggcc
     6961 ggcggcggcc tcgcgcaggg cgggcaggtc ggccaccttg ccgtcggcgg actgcgcggc
     7021 gctgacggcg acgagcgcgg tgccgggacg gacggattcg gcgatccgct ccacgggcac
     7081 cgcgcgcacc ttgagatcgc cccggacgtg gaacggggtg ggcacggagg cgaagtcgtc
     7141 ctcggcggtg aggacttcgg cgccggacgg cagcgaggcg gcgatcaccg cgctgtgggc
     7201 ggcgaccgag gcgccgagcg cgacccgttc gaccgggacc ccggcgagcc gggcgtaggc
     7261 ggcccggcac acctccacct cgtcccagag cgcggtcagc ggctgtccct gcgcccgcag
     7321 cagcgccgat tcgtgcaggg cggtgacggc gcgggcgggc agcaggccgt tgctggcggt
     7381 gttgaggaag gtgccgaccg gggcgaactc ggcgcggacg aggctctcga acgtctccgt
     7441 ggtctccatg actccactgt gcggcgcccg ggtgccgtcg tccatcacgc atctctacag
     7501 ggtgtcccca agcaaccctt atgcgtcagc cctgctgggg caccgcgcag ccgtcggggc
     7561 cacaggtgcc ggcgtcgtcc ccggtgaccg tcctgagcgg ggagcggtcg ccccacgcct
     7621 gggcgagggc ctgggtgaag acctcggcgg gctgggcgcc ggagacgccg taggtgcggt
     7681 cgaggacgaa gaaggggacg ccgttcgcgc cgagctgggc ggcctcgcgt tcgtcggcgc
     7741 ggacctcgtc ggcgtaggcg gtggggtcgg cgaggaccgc gcgggtggcg tccgcgtcca
     7801 ggccggcctc ggcggccagc tccaccaggt ggtcgtcgcc gccggtgaac acggactgct
     7861 cgtcggcgaa gttggcccgg tagagccggt ccagcagcgc ctcctgcctg ccctgctcct
     7921 tggcgaagtg cagcaggcgg tgcaggtcga aggtgctgcc gtggtcgcgg ccgcgggtgc
     7981 ggtagtccag gccctcggcg gcggcctggg cgccgaggtt gtcctcgccg gcctcggcct
     8041 gggccgcgct catgccgtac ttgcgggtca gcatgtcgag caccggctgt acgtcgccct
     8101 tggcgcggcc ggggtccagc tcgaaggaac ggtgcacgac ctcgacctcg tcccggtgcg
     8161 ggaacgcggc gagcgccttc tcgaagcgtg ccttgccgac gtagcaccag gggcaggcga
     8221 tgtcggacca gatctcgacg cgcatcttca gggctctctt ccgctcgtac cggacgggga
     8281 ctctctccgc ccggtacgtg aacgttcaag cagcttggtt cattcccccg gcggggtcca
     8341 gcgcaggaac aggtggtggt cgtcctcggc gggcggcttc gccgggtcgg ggacccagcc
     8401 ctggcgggcg tagaacgcct gggcgcgccg gttgtcgacg tgcacgtcga gcacggcggc
     8461 ccgcacgcgg tcggccagcc agcgctcgac gcaggcggcg tgcagggcgg cgccgaggcc
     8521 cgcgcgccag tggtccgggg acacatggaa ctggtacagc ttcaccgtgt ccaccggctc
     8581 gccctccggg gtgcggaagg aggcgacggc gacgatctcc ccgtcccgca ccccgcacag
     8641 cacctggccg tcggggcgct cgatggagcc ccgccaggca tccgtccagt cgaagtccga
     8701 cggaggcagc ccgtccgggt agtacgtcgc acgggcccgc gcgtgcaggg cggcgatgat
     8761 ctcggcctcc cccggctcgg ccggacggat caccagggcg tccgctgtct cgtcactgat
     8821 catgaggttc aggacgtacg gggcacacgc gcggttccgc cctcagcttc cgtcgcgcag
     8881 gtccgcgggc caggtgggcc ggaattccag gtggtcgtag gtcaccacgc agccctcgcc
     8941 gagcggtgac tgggtcatga agccgatcag ggcggcgccg gtctccttct cgtcgccgag
     9001 ggtgaagagg cggacgaagg tccagcgttc gccgtcgcgg gaggcgtgga acgccagcgc
     9061 ccggccggtg cggctcaccc ggagccagac cgaactgccg ggcacggtga aggagttggc
     9121 gtcgtcggag tggccccggg tgaccaccgt gcagatggtg ggcacgtccg gggagttctc
     9181 caggcagagc ttggcccagt tccggtcgcc gacgtggacg tagagcaccc cggcgtcgaa
     9241 ggccgcgttg aagccgaccg tgacgcgggc gatcagctgg aagtcgccct cgggcgagcc
     9301 gagcagccgg ggcgcgtcgg cggccgggtc cagcgcctct ccggtcggcg tgacgaaccg
     9361 gtcctgccgg ggcccggccc agccggtgag cactcccttc tcgtacgacc agtggccgtc
     9421 cgggccgtac gtccgcaaag ggaaggggag ttcgggaagc tctatgtcca tcgggcgatt
     9481 ctcgcagcct gttcgcgtca gcgttcgagc actccgtcgt aacgccgggg cagacgcagc
     9541 gggttgtcgt cccgcagctc gggcgggagc aggggctcgg gggtgttctg gtaggcgacc
     9601 gggcgcagcc agcgttcgac ggcggtgccg ccgaccgagg tcgaggtgga ggtggtggcg
     9661 gggtaggggc cgccatggtg ctgggccggg gccaccgcga ccccggtcgg ccagccgtcg
     9721 accaggaccc ggcccgccag cggggtcagc cgggccagca gcgcggccgc gtccccggcc
     9781 cccgccgcct cggccgggga gagctgcacg gtcgcggtga gactgcccgg cagccgctcc
     9841 agtacggcgg tcagctcgtc ctcgtccgcg tagcgggcca ccacggtgac cgggccgaag
     9901 cactcctcca gcagcggttc gtacgcgtcc tcggtgagcc gggccgcggg gacggtgagg
     9961 aagcccgcgc cgaccgtgtg ggctccggcc gcgcccggcg tgacggggga ctccacctcg
    10021 ggcagggccg cgcgtgcgcc gacacccgcg aggaaggcgt cccgcatgcg gtggtcgagc
    10081 agtacgccgg gctcggtgtc gctgacggcg tcggtgagcg ccttgacgag gccgtcgccg
    10141 tgggtcccgg cgggcaccag caccaggccg ggcttgacgc agaactggcc gacaccgagg
    10201 gtcatggagg cggcgagtcc cgcgccgatg tcctgggccc gctcggcggc cgcgtcctcg
    10261 gtgaccacga ccgggttgag cgagccgagt tcgccgtgga acgggatcgg caccgggcgg
    10321 gcggcggccg cgtcgaacag ggcccggccg ccgcgcaccg acccggtgaa gcccgcggcg
    10381 gcgaccaggg ggtgcttgat caggt
//
