LOCUS       NZ_SHKU01000005        11287 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. BK022 Ga0307664_105, whole genome shotgun sequence.
ACCESSION   NZ_SHKU01000005
VERSION     NZ_SHKU01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. BK022
  ORGANISM  Streptomyces sp. BK022
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 607918)
  AUTHORS   Kalluri,U.
  TITLE     Understanding the reciprocal impacts of modified plant cell wall and
            associated microbiome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 607918)
  AUTHORS   Kalluri,U., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 215.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004217355.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 02:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,482
            CDSs (total)                      :: 6,401
            Genes (coding)                    :: 6,280
            CDSs (with protein)               :: 6,280
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 49 of 121
            Pseudo Genes (incomplete)         :: 92 of 121
            Pseudo Genes (internal stop)      :: 16 of 121
            Pseudo Genes (multiple problems)  :: 29 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:05:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 281952
            Orig. end                         :: 293239
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SHKU01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11287
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11287
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11287
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6287](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6287
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            41..619
                     /locus_tag="EV284_RS22395"
                     /old_locus_tag="EV284_4491"
     CDS             41..619
                     /GO_function="GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824170.1"
                     /locus_tag="EV284_RS22395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4491"
                     /product="carbonic anhydrase"
                     /protein_id="WP_130337008.1"
                     /transl_table=11
                     /translation="MQPLIDYARTFGRRPQEFARLAEGQSPQVLFITCSDSRVVPALIT
                     GARPGELFELRTAGNIVPPHTSEHPTSEAATIEYAVEVLGVRDIVVCGHSHCGAVGALV
                     RGDDLTAVPAVRDWLAHAAPRPAGEAGDPEVAEAVQCHALTQLLRLRSYPCVERRTAEG
                     TLALHAWYYEVHTGAVRAHRPGTDTFEAL"
     gene            628..2136
                     /locus_tag="EV284_RS22400"
                     /old_locus_tag="EV284_4492"
     CDS             628..2136
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675668.1"
                     /locus_tag="EV284_RS22400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4492"
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_130337010.1"
                     /transl_table=11
                     /translation="MTGQGLISRAPHLRRDFAASLVVFLVALPLCVGVAVASGVPAELG
                     LVTGIVGGLVTGVLRGSSLQVSGPAAGMTVLVFEAVKEFGLPVLGAIVLATGLLQIGMG
                     VLRLGRYFRAISLAVVEGMLAGIGLVLMAGQLYPAMAAKAPDTGLDKMLGLPRGLAGAV
                     GDGRALASLALCAGTIAVMVGWKHLPARVRAVPGPLAAVGLATLAVPVFGMRVAMVEVQ
                     GLLGALQPPPLGAFAEFADVAVLGTIVAFTLIASAESLFSAAAVDRLHDGPRTEYDREL
                     TAQGVGNTVCGLLGALPLTAVIVRSAANVQAGARTKASRVMHGVWLLLFAALLPGALAH
                     IPLPSLAGVLIYSGAKLVPVKALAGLWREQRGEALILAVTAVSIVAVGMFEGVLVGLAL
                     AIVKTAWEASHIKVTVIDKGAGPVDAYLSGNATFLRLPKILDSLEALPKDRPVRLDLSG
                     LLHLDHACRTALENWAERHSADGTEPVRLTSGAGAGEPAGTPAG"
     gene            2296..3213
                     /locus_tag="EV284_RS22405"
                     /old_locus_tag="EV284_4493"
     CDS             2296..3213
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643058.1"
                     /locus_tag="EV284_RS22405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4493"
                     /product="slipin family protein"
                     /protein_id="WP_130337012.1"
                     /transl_table=11
                     /translation="MWELVITLIAVAGAAGLVLLAAAMRVVKQYERGVLFRLGRITGEP
                     RTPGLTLIIPFVDRLHKVNMQIVTLPIPAQEGITRDNVTVRVDAVVYFRVVDPTSALVR
                     VEDYKFAVSQMAQTSLRSIIGKSDLDDLLSNREKLNEGLELMIDSPAVGWGVQIDRVEI
                     KDVSLPDTMKRSMARQAEADRERRARIINADAELQASKKLAEAAQQMSDTPTALQLRLL
                     QTVMAVSAEKNSTLVLPIPVELLHFLERGARDPAPARETRKEPAPEPAPPVEPVPVAGA
                     PDLDAVTRTPELGAILDQPPTEVR"
     misc_feature    complement(3258..3271)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.46"
                     /tool="antismash"
     gene            3300..4154
                     /locus_tag="EV284_RS22410"
                     /old_locus_tag="EV284_4494"
     CDS             3300..4154
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675664.1"
                     /locus_tag="EV284_RS22410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4494"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_130337015.1"
                     /transl_table=11
                     /translation="MIALARRLTAVCVLGAALAACGTATPAKPVRPAHAEPHASASPSP
                     SGTPTMAAGPGGLTPVFEHGPRDRGKTVALTFDADMTADEGPRAAAGERFDNPGLISAL
                     RTLKVPATVFMTGRWAEEYPDQARALGHDAQFEVANHSYSHYAFTGDCYGLPTVAADAQ
                     LTEVQRTYGALRKAGVPHPMPYFRFPGGCYDRDALRAISGAGVTAVQWDVISGDAFATD
                     ADAVVRQVLDGVKPGSVVVMHCTRSAAPTTEKVVRSVVPELRRKGYRFVKVSDLVRESG
                     TPR"
     gene            4187..4903
                     /locus_tag="EV284_RS22415"
                     /old_locus_tag="EV284_4495"
     CDS             4187..4903
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125241.1"
                     /locus_tag="EV284_RS22415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4495"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_165425614.1"
                     /transl_table=11
                     /translation="MTHVPAAPGDSPFQTEPTPRDEAPQFVLPLVVRIEKSAPPARTDA
                     LETAARAVLVLLGDERARGDGEWAGAVRAWEDARIRKVVRRARGAEWRRAEELPGITVT
                     GAGAEVRVFPPIPLDGWPKELAKLQVSGTELDDPEPPAAPDGTAPVLWLNPELEMSAGK
                     EMAQVGHGAQLAWWALSDAAREAWRTAGHPLAVRTATPARWRELTDGHLPLVRDAGFTE
                     IAPGSATVVADHPALH"
     misc_feature    complement(4914..4923)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.81"
                     /tool="antismash"
     gene            5001..6287
                     /locus_tag="EV284_RS22420"
                     /old_locus_tag="EV284_4496"
     CDS             5001..6287
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824195.1"
                     /locus_tag="EV284_RS22420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4496"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_130337019.1"
                     /sec_met_domain="DUF692 (E-value: 1.1e-94, bitscore: 311.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGTGIGWRPEIADAVAGMAGIDWVEVVAENVCPGHLPAPLVRL
                     RERGVTVVPHGVSLGLGGAERPDEGRLRALAERAEALGAPLVTEHIAFVRAGGPLTASA
                     PLEAGHLLPVPRTRDALDVLCENVAIAQEALPVPLALENIAALFSWPDEEMSEGEFLAE
                     LVRRTGVRLLIDVANLHTNHVNRGEDPAEALDRLPLEALAYVHVAGGFERDGVWHDSHA
                     HPVPPLVLDILTDLASRVAPPGVLLERDENFPRPAELERELADIGEAVRKGGGAARTPR
                     SETFPVPPAASAEARQRLAVAQTAVLSALVARTPLPEGFDRGRMGVQARSLAAKRADVV
                     AKVAPELPVILGAGYREAFLGYARTRPMRGGYRQDALEFAAQLLKTGRPADARARRRLR
                     EWWLDRAGPAPRTSGPAARLARRVLSGRR"
     misc_feature    6361..6370
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 12.92"
                     /tool="antismash"
     gene            6396..7190
                     /locus_tag="EV284_RS22425"
                     /old_locus_tag="EV284_4497"
     CDS             6396..7190
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122142.1"
                     /locus_tag="EV284_RS22425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4497"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_130337021.1"
                     /transl_table=11
                     /translation="MFWVLLLLPAWAFAAIACTRLCLAAVRATAADRAAGADGHDRDLT
                     LYETAFLSGGPSRVADLAMVRMARQRRLLLAHTGWATVVDPRGQDEMERSVIGAIGPQG
                     QSRIAPVRAAAAGTEAVQRLAERLIAAGLAVPFDTGTTVAAGVRQVRAAAVAVLALGAA
                     ALLMPTPVDVPRFQIAMWFALPLVLTLSCLAIARVEVHPYPRWASPTGQRLLGEVARRP
                     AGDGDEGSFLMRLATRGVRALNEPELRAAFTHRDAPGAHREH"
     gene            7308..8930
                     /locus_tag="EV284_RS22430"
                     /old_locus_tag="EV284_4498"
     CDS             7308..8930
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (107-481). E-value:
                     1.1e-19. Score: 62.9. Matches aSDomain:
                     nrpspksdomains_EV284_RS22430_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547189.1"
                     /locus_tag="EV284_RS22430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4498"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_130337023.1"
                     /sec_met_domain="PF00561 (E-value: 1.4e-25, bitscore: 85.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAAGSLLLTGLAVTPAGGTPGTPGAAELRGTAVAAARA
                     HAAGLAFGACPDDSPAGMRCATVRVPLDYARPGGAQIELTVSRMPATQRDPANSKRKVP
                     RQGALVYNPGGPGASGLNFPMIGVIPEWRRIASAYDLVGYAPRGVAPSAPLSCEKPEEF
                     FKAPSLAPVHPSEAYKRERIARAKAYARGCAERSGSGLEFYNSLDNARDLDVLRAALGE
                     SRLTFMGASYGTYVGALYASLFPGHVRRMVLDSAVDPDPAKVWYRENLDQSAAFEDRWA
                     DFRAWVARHDDVYGLGATPAAVQRSYDTASDRLQAHPADGKVGPGQLQNTFLMAGYYDD
                     YWPSRAAALAAYLKGDPKPLVRLGAPSPETAAEAENGSAVYTAVECNDAPWPADFRVWD
                     RDNTRLARTAPFETWDNAWTNLPCAYWPARRQVPLDVRTGPGETPPVLILAAERDAATP
                     YDGALELHRRLAGSTLVTERDAGTHGIGGGPNRCVNAHLDAYLLEGRLPGRRAACAPRP
                     EPRPTEPAGRGTPDRDALKDKGA"
     aSDomain        7629..8750
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EV284_RS22430_Abhydrolase_1.1"
                     /evalue="1.10E-19"
                     /label="EV284_RS22430_Abhydrolase_1.1"
                     /locus_tag="EV284_RS22430"
                     /protein_end="481"
                     /protein_start="107"
                     /score="62.9"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASGLNFPMIGVIPEWRRIASAYDLVGYAPRGVAPS
                     APLSCEKPEEFFKAPSLAPVHPSEAYKRERIARAKAYARGCAERSGSGLEFYNSLDNAR
                     DLDVLRAALGESRLTFMGASYGTYVGALYASLFPGHVRRMVLDSAVDPDPAKVWYRENL
                     DQSAAFEDRWADFRAWVARHDDVYGLGATPAAVQRSYDTASDRLQAHPADGKVGPGQLQ
                     NTFLMAGYYDDYWPSRAAALAAYLKGDPKPLVRLGAPSPETAAEAENGSAVYTAVECND
                     APWPADFRVWDRDNTRLARTAPFETWDNAWTNLPCAYWPARRQVPLDVRTGPGETPPVL
                     ILAAERDAATPYDGALELHRRLAGSTLVTERDAG"
     gene            complement(8927..9658)
                     /gene="hemQ"
                     /locus_tag="EV284_RS22435"
                     /old_locus_tag="EV284_4499"
     CDS             complement(8927..9658)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="EV284_RS22435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4499"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_130337025.1"
                     /transl_table=11
                     /translation="MSDDAPTTEPARIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAETADQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYEWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWMLAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFYTGRRKSVAELVAGLA"
     gene            complement(9664..11109)
                     /gene="hemG"
                     /locus_tag="EV284_RS22440"
                     /old_locus_tag="EV284_4500"
     CDS             complement(9664..11109)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547191.1"
                     /locus_tag="EV284_RS22440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4500"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_130337027.1"
                     /sec_met_domain="DAO (E-value: 5.6e-11, bitscore: 37.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAASKGHVVVIGAGVAGLAAAHRALARGARVTVLEAADRVGGKL
                     LPGEIAGLRVDLGAESMLARRPEAAGLAREVGLGDRLRPPSTATASVWTRDALRPMPKG
                     HVMGVPGTASALSGLLTAEGLARIERDAELPPAEIGDDVAVGEFVAARMGREVVDRLVE
                     PLLGGVYAGDAYRLSLRSAVPQLYQAARTHDTLTAAVRSLQAKAAEAAQTGPVFLGIEG
                     GVGTLPLAVADSVRARGGEILTRSPVSELRRDGDGWRVTAGDRTLRADAVVVAVPAPAA
                     SALLRAEAPAAAAELAAVEYASMALITLVYRRADAVLPEGSGFLVPPVDGRTIKAATFA
                     SQKWGWVGAEDPDLLVVRTSVGRHHETEILERDDAELVAVARHDLRAATGLDARPVATR
                     VTRWTDGLPQYPVGHHARVARVREHIAKLPGLAVCGAQYDGVGIPACVASAHAAVDQLG
                     GDLAGVRELTANPVQSLHGGAGE"
ORIGIN
        1 ccgacgcgcc gccgaggcgc gccaggagga gtccaccatc atgcagcccc tcatcgacta
       61 cgcccgcacg ttcggccggc gcccccagga gttcgcccgc ctcgccgagg gacagtcccc
      121 ccaggtcctg ttcatcacct gctccgactc acgggtcgtc ccggccctga tcacgggcgc
      181 ccgccccggc gagctgttcg agctgcgcac ggcgggcaac atcgtcccgc cgcacacctc
      241 cgagcacccc accagcgagg ccgccaccat cgagtacgcc gtcgaggtgc tcggcgtgcg
      301 cgacatcgtc gtctgcggac actcgcactg cggcgcggtc ggcgcgctgg tgcgcggcga
      361 cgacctgacc gccgtacccg ccgtgcgcga ctggctggcg cacgccgccc cgcgcccggc
      421 cggcgaggcc ggggacccgg aggtcgccga ggccgtgcag tgccacgcgc tgacccagct
      481 gctgcgtctg cgctcgtacc cgtgcgtcga gcgccggacg gcggagggca cgctcgccct
      541 gcacgcctgg tactacgagg tccacaccgg ggccgtgcgc gcgcaccggc ccggcaccga
      601 caccttcgaa gccctgtgag cgcgccgatg accgggcaag ggctcatatc gcgcgctccg
      661 catctgcggc gggacttcgc cgcgtccctc gtcgtcttcc tcgtcgcgct cccgctgtgc
      721 gtgggggtgg ccgtcgcctc cggggtgccg gccgaactcg ggctggtcac cggcatcgtg
      781 ggcggtctcg tcaccggcgt gctgcgcggc agcagtctcc aggtgtcggg cccggcggcc
      841 ggcatgaccg tgctggtctt cgaggcggtc aaggagttcg gactgccggt gctgggcgcg
      901 atcgtgctcg ccaccgggct gctccagatc gggatgggag tgctgcggct cggccggtac
      961 ttccgggcca tatcgctggc ggtcgtggaa ggcatgctgg cgggaatcgg tctggtcctg
     1021 atggccggac agctgtatcc ggcgatggcc gccaaggctc cggacaccgg gctggacaag
     1081 atgctcggtc tgccgcgcgg tctcgcgggc gcggtcggcg acggcagggc actggcctcg
     1141 ctggcgctgt gcgccggcac catcgccgtc atggtggggt ggaagcacct cccggcgcgg
     1201 gtgcgtgcgg tgcccggtcc gctcgccgcc gtcgggctgg ccacgctggc cgtgccggtg
     1261 ttcggcatgc gggtggcgat ggtcgaggtg caggggctgc tgggcgccct ccagccgccg
     1321 ccgctcggcg ccttcgccga gttcgccgat gtggcggtgc tcggcacgat cgtcgcgttc
     1381 accctgatcg cgtccgccga gtcgctgttc agcgcggcgg cggtggaccg gctgcacgac
     1441 ggcccgcgca ccgagtacga ccgtgagctg accgcccagg gcgtcggcaa cacggtgtgc
     1501 gggctgctcg gcgcgctgcc gctgaccgcg gtgatcgtgc gcagcgcggc caatgtgcag
     1561 gcgggcgcgc ggaccaaggc gtcccgcgtg atgcacggtg tctggctgct gctgttcgcg
     1621 gcgctgctgc cgggcgccct cgcccacatc ccgctgccgt cgctggcggg cgtcctgatc
     1681 tactcggggg ccaagctggt gccggtcaag gcgctggcgg ggctgtggcg cgagcagcgc
     1741 ggcgaggcgc tgatcctggc ggtgacggcg gtgtccatcg tggcggtcgg catgttcgag
     1801 ggcgtgctgg tcgggctggc gctggcgatc gtgaagaccg cctgggaggc gtcgcacatc
     1861 aaggtcaccg tcatagacaa gggcgcgggc cccgtggacg cctatctgtc gggcaacgcg
     1921 accttcctca ggctgccgaa aatactggac agcctggagg cgctgcccaa ggaccgcccg
     1981 gtgcggctgg acctctccgg cctgctgcac ctggatcacg cctgccgcac cgccctggag
     2041 aactgggccg agcggcacag cgcggacggt acggaaccgg tacggctgac gtccggagcc
     2101 ggggcgggtg agcccgccgg cacccccgcg ggctgagccg ggccggccgg gggccggggt
     2161 gacgggagcc ttcccgccgc cccggccccc ttttcgcctg aaaggttccc tgtccacagc
     2221 ccgtagcttc cgcacacatc cgggacgtac tgttgagtaa gggattaaac ggacctctcg
     2281 atgagggggg cgaccatgtg ggagctcgtg atcacgctca tagcggtggc cggcgctgcg
     2341 ggtctcgtgc tgctggccgc ggcgatgcgt gtcgtcaagc agtacgaacg gggagtgctg
     2401 ttccggctgg gacggatcac cggtgagccc cgcaccccgg ggctgacgct gatcattccg
     2461 ttcgtggacc ggctgcacaa ggtgaacatg cagatcgtca cgctgccgat cccggcccag
     2521 gagggcatca cccgggacaa cgtcaccgtg cgggtggacg cggtcgtcta cttccgggtc
     2581 gtcgacccga ccagcgcgct ggtgcgggtg gaggactaca agttcgcggt gtcgcagatg
     2641 gcgcagacct cgctcaggtc gatcatcggc aagagcgacc tggacgatct gctgtccaac
     2701 cgggagaagc tcaacgaggg cctggaactg atgatcgaca gcccggcggt gggctggggc
     2761 gtacagatcg accgggtcga gatcaaggac gtctccctgc cggacaccat gaagcgctcg
     2821 atggcccgcc aggccgaggc cgaccgggag cgccgggcgc gcatcatcaa cgcggacgcg
     2881 gaactccagg cgtccaagaa gctggccgag gccgcgcagc agatgtcgga caccccgacc
     2941 gccctccagc tccggctgct ccagacggtg atggcggtgt ccgccgagaa gaactcgacc
     3001 ctggtcctgc ccatcccggt ggagctgctc cacttcctgg aacggggcgc ccgcgatccg
     3061 gccccggcca gggagacgag gaaggagccc gccccggagc cggcaccgcc cgtcgagccc
     3121 gtccccgtgg ccggagcgcc ggacctcgac gccgtcacca ggacacctga actcggcgcg
     3181 atcctcgacc agccgcccac cgaggtgagg tgacacccca cccgccgcgc gagtgacccg
     3241 ctcggggcgt cggggggtgg atgaacatac gatcagtcag acagccctat tttcatacgg
     3301 tgattgcact cgcacgccgt ctcaccgccg tctgcgtcct cggcgcggcc ctcgccgcct
     3361 gcggtaccgc gacccccgcc aagcccgtac gcccggcgca cgccgagccc cacgcctccg
     3421 cctccccctc cccctccggc acccccacca tggcggcggg tccgggcggc ctcacccccg
     3481 tgttcgagca cggcccgcgc gacaggggca agaccgtcgc gctgaccttc gacgccgaca
     3541 tgaccgccga cgaggggccc cgcgccgccg ccggtgagcg cttcgacaat cccggtctga
     3601 tcagcgccct gcgcacgctg aaggtcccgg cgaccgtgtt catgaccggc cgctgggcgg
     3661 aggagtaccc ggaccaggcc cgcgccctcg gacacgacgc ccagttcgag gtggccaacc
     3721 actcctacag ccactacgcg ttcaccggtg actgctacgg cctgccgacc gtcgccgcgg
     3781 acgcccagct gaccgaggtc cagcgcacct acggcgcgct gcgcaaggcg ggcgtgccgc
     3841 acccgatgcc gtacttccgc ttccccggcg gctgctacga ccgggacgcg ctgcgcgcca
     3901 tcagcggcgc cggtgtcacc gccgtccagt gggacgtgat cagcggcgac gccttcgcca
     3961 cggacgccga cgcggtcgtg cgccaggtgc tggacggggt gaagcccggt tcggtggtcg
     4021 tcatgcactg cacccgcagt gccgccccga cgaccgagaa ggtcgtccgc tcggtggtgc
     4081 cggagctgcg caggaagggc taccgcttcg tgaaggtctc ggacctggtc cgggagtccg
     4141 gtaccccgcg ctgaccggcg ccggactgct cgtagggttg gctgacgtga cccatgttcc
     4201 agccgccccc ggcgacagcc ccttccagac cgagcccacc ccgcgcgacg aggcaccgca
     4261 gttcgtgctg cccctcgtcg tacggatcga gaagagcgcg ccgcccgccc gtaccgacgc
     4321 gctggagacc gccgcacgcg ccgtgctcgt cctgctcggg gacgagcggg cgcgcggtga
     4381 cggcgagtgg gccggggcgg tgcgggcctg ggaggacgcc cgcatccgca aggtggtccg
     4441 gcgggcgcgg ggcgcggagt ggcgccgcgc cgaggagctg cccggcatca cggtcaccgg
     4501 cgccggcgcc gaggtacgcg tcttcccgcc gatcccgctg gacggctggc ccaaggagct
     4561 ggccaagctc caggtctccg gcaccgaact ggacgacccg gagccgcccg ccgcccccga
     4621 cggcaccgcc cccgtgctct ggctcaaccc ggagctggag atgtcggccg gcaaggagat
     4681 ggcccaggtc ggccacggtg cccagctcgc ctggtgggcg ctgtccgatg cggcccgcga
     4741 agcctggcgt accgccggcc accccctcgc cgtccgcacc gccaccccgg cccgctggcg
     4801 tgaactcacc gacggccacc tccccttggt ccgcgacgcg ggcttcacgg agatcgctcc
     4861 cggctccgcc acggtggtcg ccgaccaccc ggcgctgcac tgaccggcga acctcaaata
     4921 ttgctctgaa ccccccgccc cggcggttcg cccgcccccg ccggggccat acccccggca
     4981 gacgaaggag ggggacgggg atgcggctcg ggacggggat cgggtggcgg ccggagatcg
     5041 cggacgccgt ggcggggatg gcggggatcg actgggtgga ggtcgtcgcc gagaacgtct
     5101 gccccggcca tctgccggcg ccgctggtgc ggttgcggga gcgcggggtg acggtggtcc
     5161 cgcacggggt ctccctcgga ctcggtggtg ccgagcggcc ggacgagggg cggctgcggg
     5221 cgctggccga gcgggccgag gcgctggggg cgccgctggt caccgagcac atcgcgttcg
     5281 tccgcgcggg cggaccgctc accgcctcgg cgccgctgga ggcgggccac ctgctgcccg
     5341 taccgcgcac gcgggacgcc ctcgacgtgc tgtgcgagaa cgtggcgatc gctcaggagg
     5401 cgctgccggt gccgctggcg ctggagaaca tcgccgccct gttctcctgg ccggacgagg
     5461 agatgagcga gggcgagttc ctggccgagc tggtccggcg gaccggcgta cggctgctga
     5521 tcgacgtggc caacctgcac accaaccacg tcaaccgggg cgaggacccc gccgaggcac
     5581 tggaccggct cccgttggag gcgctcgcct atgtgcacgt cgcgggcggc ttcgagcggg
     5641 acggcgtctg gcacgacagc cacgcgcacc ccgtgccgcc cttggtgctg gacatcctga
     5701 ccgacctggc gtcgcgcgtc gcaccgcccg gcgtcctgct ggaacgggac gagaacttcc
     5761 ctcgaccggc tgagctggag cgggagctgg ccgacatcgg ggaggcggtg cgcaagggcg
     5821 gcggggccgc gcgaactccc cggagcgaga cctttcccgt cccgcccgcg gcctccgccg
     5881 aggcacggca gcggttggcc gtcgcacaga cggccgtgct gtcggcgctg gtggcgcgca
     5941 cgccgctgcc cgaggggttc gaccgggggc ggatgggcgt acaggcgcgg tcgctggcgg
     6001 cgaagcgggc ggacgtggtg gccaaggtgg cacccgagct gccggtgatc ctcggcgccg
     6061 ggtaccggga ggcgttcctc ggctacgcgc gcacccgccc gatgcgcggc ggataccggc
     6121 aggacgcgct ggagttcgcc gcccagctcc tcaagacggg gcgcccggcg gacgcgcggg
     6181 cgcgcaggag gctgcgggaa tggtggctcg accgcgcggg tccggcaccc cgtacctccg
     6241 gcccggccgc ccggctggcc aggcgggtgc tgtcgggccg gcgctgacca ccctccccga
     6301 gccgcggaaa aacggaacgt cacgtaccgc taacgctctg tccggatagt gaacaaccca
     6361 tggtttttca ggggccgttg gcatagaaaa atctcatgtt ctgggtcctc ctcctgctcc
     6421 cggcctgggc cttcgccgcc atcgcctgca cccggctctg cctggccgcc gtccgcgcga
     6481 ccgccgcgga ccgcgcggca ggggccgacg gccacgaccg cgacctgacg ctgtacgaga
     6541 cggcgttcct gtccgggggc ccctcccggg tcgccgacct cgccatggtc cggatggccc
     6601 ggcagcgccg gctgctgctc gcgcacaccg gctgggccac cgtggtggac ccgcgcgggc
     6661 aggacgagat ggaacgttcc gtcatcggcg cgatcggccc gcagggacag tcccggatcg
     6721 cccccgtaag ggcggccgcg gccggtacgg aggccgtgca gcggctcgcc gagcggctga
     6781 tcgcggccgg gctggcggtc cccttcgaca ccgggacgac cgtggccgcg ggggtgcgtc
     6841 aggtgcgggc agccgccgtg gcggtgctcg cgctgggcgc ggccgcactg ctcatgccga
     6901 cccccgtgga cgtgccgcgc ttccagatcg cgatgtggtt cgcgctgccc ctcgtcctca
     6961 ccctgagctg cctggccatc gcccgcgtcg aggtgcatcc ctacccgcgc tgggcctccc
     7021 ccaccgggca gcggctcctc ggggaggtgg cccggcgtcc ggcgggggac ggcgacgagg
     7081 gctccttcct catgcggctc gcgacgcggg gcgtgcgcgc gctgaacgag ccggagctgc
     7141 gggccgcgtt cacccaccgc gatgcgccgg gggcgcacag ggagcactga cgccgacacc
     7201 cgcgtgccgg tgcttgcctt tcccgaggcc tcttccgaaa catcccttct gtcgcggccg
     7261 ggccatggca gccgtgccat cgccgccgcg caccgaaggg atgtccgatg agagctgccg
     7321 ccctgtactc ggccgccggt tccttgctgc tgaccgggct cgccgtgacc ccggccggtg
     7381 gtacccccgg cacaccgggc gcggccgagc tgcgcggcac cgccgtcgcc gccgcccggg
     7441 cccacgcggc cggcctcgcc ttcggcgcct gcccggacga ctctccggcc gggatgcgct
     7501 gcgccaccgt acgcgtcccg ctggactacg cccgccccgg cggcgctcag atcgagctga
     7561 ccgtcagccg gatgccggcc acccagcgcg acccggccaa cagcaagcgc aaggtgcccc
     7621 ggcagggcgc cctcgtctac aaccccggcg gccccggcgc ctccggcctg aacttcccga
     7681 tgatcggcgt gatccccgag tggcggcgca tcgcctccgc ctacgacctg gtcgggtacg
     7741 ccccgcgcgg ggtcgcgccc tcggccccgc tgtcctgcga gaagccggag gagttcttca
     7801 aggccccctc gctcgccccg gtccacccga gcgaggcgta caagcgggag cggatcgcgc
     7861 gggcgaaggc gtacgcgcgc ggctgcgccg aacgctccgg cagcgggctg gagttctaca
     7921 actcgctcga caacgcccgt gacctggacg tcctgcgggc cgcgctcggc gagtcccggc
     7981 tgaccttcat gggcgcctcg tacggcacgt atgtgggcgc gctgtacgcc tctctcttcc
     8041 ccggtcacgt acggcggatg gtgctggact cggcggtgga cccggacccg gcgaaggtct
     8101 ggtaccgcga gaacctggac cagtcggccg cgttcgagga ccgctgggcc gacttccggg
     8161 cctgggtggc ccgccacgac gacgtgtacg gcctcggcgc gaccccggcc gccgtccagc
     8221 gctcctacga caccgcgagc gaccggctcc aggcgcaccc ggcggacggc aaggtcggtc
     8281 cgggccagct ccagaacacg ttcctgatgg cggggtacta cgacgactac tggccgagcc
     8341 gggccgccgc tctcgccgcc tatctgaagg gcgacccgaa accactggtc cggctgggcg
     8401 cgcccagccc ggagacggcc gccgaggcgg agaacggcag cgcggtgtac acggccgtcg
     8461 agtgcaacga cgcgccctgg cctgccgact tccgggtctg ggaccgcgac aacacccgcc
     8521 tcgcccgcac cgcccccttc gagacctggg acaacgcgtg gacgaacctg ccgtgcgcgt
     8581 actggcccgc gcgccgtcag gttccgctcg acgtgcgcac cggccccggc gagaccccgc
     8641 cggtgctgat cctggcggcc gagcgggacg cggccacccc ctacgacggg gcgctggagc
     8701 tgcaccggcg gctggccgga tccacgctgg tcaccgagcg ggacgcgggc acgcacggca
     8761 tcggcggcgg ccccaaccgg tgtgtcaacg cccacctgga cgcctacctg ctcgaaggcc
     8821 gcctgccggg ccgccgcgcc gcctgcgccc cgcgcccgga gccgcgcccc accgagccgg
     8881 ccggacgcgg caccccggac cgggacgccc tcaaggacaa gggcgcctag gcgagccccg
     8941 ccaccagttc cgccacggac ttgcggcggc cggtgtagaa cggcacctct tcgcggacgt
     9001 gcatccgggc ctcggaggca cgcaggtgac gcatgaggtc gacgatgcgg tacagctcgt
     9061 cggcctcgaa ggcgagcatc cactcgtagt cgcccaggga gaaggaggcg accgtgttgg
     9121 cccgcacgtc ggggtagccc cgggccatct tgccgtggtc ggcgagcatg cggcggcggt
     9181 cctcgtcggg cagcagatac cactcgtaag agcgaacgaa ggggtacacg ctcacgtagt
     9241 cgcggggcgt ctcgtcggcg aggaacgccg ggatgtgcga gcggttgaac tcggccgggc
     9301 ggtgcagcgc catgttcgac cacaccggct ccagcgcgcg gcccagcctg gtgcggcgga
     9361 agaggttgta cgcctcctgg agctggtcgg cggtctcggc gtgccaccag atcatcaggt
     9421 cggcgtcggc acggaggccg gagacgtcgt aggtgccgcg gaccgtgacg tccttcgcgg
     9481 cgagctggtc gaacagctcc tggacctcgt cggcgtagcc cgcgcggtcc tccggcagca
     9541 cgtccttcag cttgaagacg gaccacaggg tgtagcggat gacctcgttg aggtccttgg
     9601 ccagcttgcc cttgttcggg atgcgggccg gctcggtggt gggggcgtcg tcactcatgc
     9661 cccctattct cccgctccgc cgtgcaggct ctgcaccggg ttggccgtca gctcgcgcac
     9721 cccggcgagg tcgccgccga gctggtcgac ggccgcgtgg gcgctggcga cgcaggcggg
     9781 gatgcccaca ccgtcgtact gcgcgccgca caccgcgagg ccgggcagct tggcgatgtg
     9841 ctcgcggacg cgggccacgc gcgcgtggtg gccgaccggg tactggggca ggccgtcggt
     9901 ccagcgggtg acgcgggtgg cgacgggccg ggcgtccagc ccggtcgcgg cgcgcaggtc
     9961 gtgccgggcg accgcgacga gttcggcgtc gtcgcgctcg aggatctcgg tctcgtggtg
    10021 gcggccgacc gaggtgcgca ccaccaggag gtccgggtcc tcggcgccga cccagcccca
    10081 cttctgggag gcgaaggtcg cggccttgat cgtgcggccg tccaccggcg gcaccaggaa
    10141 tccactgccc tcgggcagga ccgcgtcggc gcggcggtag acgagggtga tcagcgccat
    10201 ggaggcgtac tccacggccg ccagctcggc ggcggccgcc ggggcttcgg cgcgcagcag
    10261 cgccgaggcg gcgggggcgg gtacggcgac cacgacggcg tccgcccgca gcgtgcggtc
    10321 cccggccgtg acgcgccagc cgtcgccgtc ccggcgcaac tcgctcaccg gactacgggt
    10381 gaggatctct ccgccgcgcg cccgtaccga gtcggcgacg gcgagcggga gggtgccgac
    10441 gccgccctcg atgcccagga agaccgggcc ggtctgcgcc gcctcggcgg ccttcgcctg
    10501 gagggaccgg acggcggcgg tgagggtgtc gtgggtacgg gcggcctggt agagctgggg
    10561 gacggccgag cgcagggaga gacggtaggc gtcgcccgcg tacaccccgc cgagcagggg
    10621 ttccaccagc cggtcgacga cctcgcggcc catccgggcg gcgacgaact cgcccacggc
    10681 cacgtcgtca ccgatctcgg cgggcggcag ttcggcgtcg cgctcgatcc gggccaggcc
    10741 ctcggcggtg agcaggccgg acagggcgga ggcggtgccg ggtacgccca tgacatggcc
    10801 cttgggcatc ggccgcagcg cgtcccgggt ccacaccgag gcggtcgcgg tggacggcgg
    10861 ccggaggcgg tcgccgaggc ccacctcgcg ggcgaggccc gccgcttcgg ggcggcgggc
    10921 gagcatcgac tcggcgccga ggtcgaccct caggcccgcg atctcgccgg gcagcagttt
    10981 gccgccgacc cggtcggcgg cctccagcac cgtcacccgc gcgccccgcg cgagcgcccg
    11041 gtgggcggcg gccagtccgg cgacaccggc tccgatgacg acgacgtggc ccttgctcgc
    11101 tgcgctcatg caccccactc tctcagaccc cgcggggcgt cccggtgccc ggtacgtcac
    11161 cggaccgtga ccgcatcggg accggaaaac gtgaccgttc ccgcaggccg ggccgtctaa
    11221 gaggaaacag caccgcgtct catcgggggg taacagacat gcgtactcag gtccgggtcc
    11281 tggccgg
//
