LOCUS       NZ_SHKU01000004        20990 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. BK022 Ga0307664_104, whole genome shotgun sequence.
ACCESSION   NZ_SHKU01000004
VERSION     NZ_SHKU01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. BK022
  ORGANISM  Streptomyces sp. BK022
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 951417)
  AUTHORS   Kalluri,U.
  TITLE     Understanding the reciprocal impacts of modified plant cell wall and
            associated microbiome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 951417)
  AUTHORS   Kalluri,U., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 215.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004217355.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 02:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,482
            CDSs (total)                      :: 6,401
            Genes (coding)                    :: 6,280
            CDSs (with protein)               :: 6,280
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 49 of 121
            Pseudo Genes (incomplete)         :: 92 of 121
            Pseudo Genes (internal stop)      :: 16 of 121
            Pseudo Genes (multiple problems)  :: 29 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:05:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 680161
            Orig. end                         :: 701151
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SHKU01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20990
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="3"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20990
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20990
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10990](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10990)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    607..621
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.79"
                     /tool="antismash"
     gene            638..1432
                     /locus_tag="EV284_RS19915"
                     /old_locus_tag="EV284_3992"
     CDS             638..1432
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433224.1"
                     /locus_tag="EV284_RS19915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_3992"
                     /product="VOC family protein"
                     /protein_id="WP_130335971.1"
                     /transl_table=11
                     /translation="MAVQSEGTPCWADAMFTDLEGAKRFYGDVLGWTFGESSSEYGNYT
                     QAYADGKAVAAVVPPMPGQEGTSQWCLYFASPDAAATAAKVEENGGEVLMEPMRVGDFG
                     TMAIAREPSGAVFGIWQGGAHEGFEAVAKPGAYCWAELYTREPEKADAFLSAVFPYRMK
                     DMADGHVDFRLFDLGADPVLGRMRMGEEFPPEVPSYVNVYFTVDDCDAAVARATERGAV
                     LRFGPMTSPFGRFAALTDPQGASFSVIDVTTTEGGMPEFKDA"
     gene            1466..2167
                     /locus_tag="EV284_RS19920"
                     /old_locus_tag="EV284_3993"
     CDS             1466..2167
                     /EC_number="3.1.26.4"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379789.1"
                     /locus_tag="EV284_RS19920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_3993"
                     /product="ribonuclease H"
                     /protein_id="WP_130335973.1"
                     /transl_table=11
                     /translation="MIGHMRERVVAACDGASKGNPGPAGWAWVVSGDDERNPARWESGP
                     LGRATNNVAELTALERLLTAVEADVPLEVRMDSQYAMKAVTTWLPGWKRNGWKTAAGKP
                     VANQDLVVRIDALLDGRSVEFRYVPAHQVDGDPLNDFADRAASQAAVVQEAAGSAHGSP
                     EPPPSPDTPAPSARRKSPRRAAGTSGSASSRTIKAKFAGRCLCGRSYAAGESIAKNAQG
                     WGHPECRTATG"
     gene            2265..3476
                     /locus_tag="EV284_RS19925"
                     /old_locus_tag="EV284_3994"
     CDS             2265..3476
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568525.1"
                     /locus_tag="EV284_RS19925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_3994"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_207229096.1"
                     /transl_table=11
                     /translation="MKVVCVGGGPAGLYLAILLKRQNPSHDITVHERAPEGSTYGWGVT
                     YWQGLMDRLRAHDPESADAIARDSVHWHEGVAHVRNLITRQPGDQGFGIGRHRLLELLA
                     ERARTLGVRLEFTSEITEPPAGADLVVAADGIGSALRTRYADAFGTHVTHGRNHFLWLG
                     TTKVFDAFTFAFVETAHGWIWAYGYPYDAGHSTCVIECSPETLTGLGLHSAGDGDGLAL
                     LEDLFAGLLDGHPLIGRASDGISVPWLNFRTLTNRTWHHDGLVLLGDAAHTTHYSIGAG
                     TTLALEDAMCLATALGEHPALPDALAAYEKRRRSELLSAQSAARYSARWYESLPRYIEL
                     PPHRMFALLGQRHSPLLPYVPPQLYYGLDKAAGQLEPLRRLKRWLGPRAARALHAARTP
                     GRSR"
     misc_feature    3515..3528
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    complement(3539..3552)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.67"
                     /tool="antismash"
     gene            3584..5071
                     /locus_tag="EV284_RS19930"
                     /old_locus_tag="EV284_3995"
     CDS             3584..5071
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546067.1"
                     /locus_tag="EV284_RS19930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_3995"
                     /product="MFS transporter"
                     /protein_id="WP_130335975.1"
                     /transl_table=11
                     /translation="MDPSAPIPEPSGAVTAPPRLRERLTIPVLASGGILMAVMQTVVVP
                     LLPDLPRLTGSSPAAVSWMVTATLLSGAVLTPVLGRAGDMYGKRRVLTSALALMTVGSV
                     MCALSSDIAVLITARALQGAAASVVPLSISILRDELPPQRRGSAVALMSSTVGIGAALG
                     LPLAALVVQYADWHTMFWLTSALGALGVAATWWAVRESPVREPGRFDVVGALGLAVALV
                     SLLLAVSQGGQWGWTSPAVLGLFAAFAVVLALWWRQQLRTARPLVDLRLVTRPRVGLSH
                     VAALLTGFAFYANSLVTAQLVQAPVASGYGLGLSIVATGLCLLPGGVTMLLFSPLSARI
                     SARRGPRITLALGAAVIACGYALRIADSRDLWMIILGATVVATGTTLAYSALPTLILRA
                     VPAAQTASANGVNVLMRTIGQAVSSAAVTAVLVHHTSLVGGLPVPTLHGYLMAFAMAGA
                     VALAACAAALTIPGDGAPDDTPRAEGATAGARALEEA"
     gene            5068..5634
                     /locus_tag="EV284_RS19935"
                     /old_locus_tag="EV284_3996"
     CDS             5068..5634
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433220.1"
                     /locus_tag="EV284_RS19935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_3996"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_130335977.1"
                     /transl_table=11
                     /translation="MTTAPAPARRDAEATKEVILRAARHLLARHAHADITLKAVAERAG
                     VSPPLVVKYFGNKDALFARVMSFDTDADDLLDAPLAGLGRHMVRHVLASQRDRGADPLL
                     RIAFAPLHGDHGDILRANFRAQVTDRLAARLTGPDAGLRAELAVAALIGVGVMYGIARG
                     PHLRESGPDEVADRYGPLVQAQLTP"
     gene            complement(5636..6520)
                     /locus_tag="EV284_RS19940"
                     /old_locus_tag="EV284_3997"
     CDS             complement(5636..6520)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006377295.1"
                     /locus_tag="EV284_RS19940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_3997"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_130335979.1"
                     /transl_table=11
                     /translation="MSDFADLDLRLVRYFTVVAEHRHFGRAATALHITQPSLSRQVRRL
                     ERELGVELLDRTPQGTRLTGAGEVFLPRARALLRAAHEAATEARAAAEPCRITVGYTTG
                     VLVTPAVREMRRRFPDADVRAAHVRWHEAREALLGHRVDALVARLPFATDGLRVSVLYD
                     EPRVVVVPLDHRLAGKESVTLDDIADEPLPRVPDPLWDAFWRIDPRPDGRPAPDGPLIG
                     DIEDKFELVASGQALAIAAAAPGMPARPDLAAIPLHGVEPCHVALATRAGDNGRLLAAF
                     RRCAESLLTGAQV"
     gene            6658..7542
                     /locus_tag="EV284_RS19945"
                     /old_locus_tag="EV284_3998"
     CDS             6658..7542
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546064.1"
                     /locus_tag="EV284_RS19945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_3998"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_130335981.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5e-09, bitscore:
                     31.5, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVFVTGATGFIGSAVVPELIGAGHEVVGLARSDDGAAALKEAGA
                     EVFRGSLDDPEGLARAADGADGVIHLAFIHDFVDFDAAGATDLRAIEAMGAVLAGSGRP
                     LVVTSGTAIAPAGRLVTEADAPAPGTHRTPSEEAAVALAERGVRGSVVRLAPSVHGPGD
                     QGFVPHLIRIAREKGVVAQVGDGANRWPGVHRLDAARLFRLVLEQAPAGTRWHAAADEG
                     VPFREIAEVIGRHLELPVVSVPPERAGEHFGFLGRFAGVDNPTSSATTRERLGWRPTGP
                     GLLADLDQGHYFE"
     misc_feature    complement(7580..7593)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.65"
                     /tool="antismash"
     gene            7608..8102
                     /locus_tag="EV284_RS19950"
                     /old_locus_tag="EV284_3999"
     CDS             7608..8102
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057087.1"
                     /locus_tag="EV284_RS19950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_3999"
                     /product="DUF6328 family protein"
                     /protein_id="WP_130335983.1"
                     /transl_table=11
                     /translation="MTEEAPHRGRDETREERADRKWGELIQEVRVAQTGVQILFGFLLT
                     VVFQPKYGQLSSTDQIIYIVTVVLGACATGALIGPVSLHRLVSGRRVKPEAVRLAGRLT
                     FVGLVLLLATMTCSLLLILRVATHDGYVPWLVGAVVCWYLVCWYGLPIWARWRHSDPED
                     R"
     gene            complement(8099..8710)
                     /locus_tag="EV284_RS19955"
                     /old_locus_tag="EV284_4000"
     CDS             complement(8099..8710)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670765.1"
                     /locus_tag="EV284_RS19955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4000"
                     /product="DUF2238 domain-containing protein"
                     /protein_id="WP_130336500.1"
                     /transl_table=11
                     /translation="MPPVVFAGVAAVGLAVSAWGARDRTTWFLETFWVLIGLPLVVVLW
                     RRFPLTGLLCGLLAAHALVLAAGGHYTYAQVPAGDWVRDALHLSRNPYDRFGHLMQGFV
                     PAILVRELLTRTSPLRGSRWLAPLTVCACLAFSAYFELLEWLAAVIGGHGADAFLATQG
                     DVWDTQWDMFCALVGSVLSLLLLSRVHDRSLAALAARTGR"
     gene            complement(8851..9885)
                     /locus_tag="EV284_RS19960"
                     /old_locus_tag="EV284_4001"
     CDS             complement(8851..9885)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433215.1"
                     /locus_tag="EV284_RS19960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4001"
                     /product="hypothetical protein"
                     /protein_id="WP_130335985.1"
                     /transl_table=11
                     /translation="MTLAIDPTETTAAAPAPLSDLVARDAREFGVYARTGGWAFALMVA
                     RSVRPGGQAAGETPKVSAKEFAELAECSPERVMRYYRAWDRAADDGLVPQFETLEPGQD
                     VQLPDSDAWLSYYSARSSATSERGTAIAEAAEAEGIRPTKALEVAENPTALRAAILADP
                     STARAARSALLDRIREDPELQAELARDVVRTDDLKKAVATESRAADKIGYVRQIAESGQ
                     IRTPAGQTVEAPAELREEAERHLSLIDGLEDDEDTGEWAVEAYDTLKTLVADTVEADPE
                     LRVRERRTKFYGSLQKATRAFEELTFDDAGDFYEDDMVRRLEELQQAIGSCLTALRGAA
                     GVRP"
     gene            complement(10001..10990)
                     /locus_tag="EV284_RS19965"
                     /old_locus_tag="EV284_4002"
     CDS             complement(10001..10990)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468028.1"
                     /locus_tag="EV284_RS19965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4002"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_242625762.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 2.7e-59, bitscore:
                     196.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFRAERIPATRVLSESAGLVRPALREAVTGLHPWLAEMAAYSFGW
                     QEGAEAGGKGVRQALAVLGARAAGADAGAGVPGAVAVELVHAFSLLHDDIMDGDATRRG
                     RPALWKAYGTGPAVLAGDALFALAVGVLADVPGGVRLLSGALADVMRGQADDLLFAARP
                     WTGPEAVTPEEYRVMAEGKTGALLGCSLALGALLGGAPRPVVAALDRAGRHAGVAFQLA
                     DDVLGIWGRAEVTGKPVGGDLREGRRTYPVLAALAAPEGARLPGLLRGHRTAEAAALIE
                     AAGGRAAALAEARAHMAAARTVLDGLPLEPGAAAELGALLDTLAHRER"
     gene            complement(10996..12036)
                     /locus_tag="EV284_RS19970"
                     /old_locus_tag="EV284_4003"
     CDS             complement(10996..12036)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987903.1"
                     /locus_tag="EV284_RS19970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4003"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_130335987.1"
                     /transl_table=11
                     /translation="MYGKAFAPEYQGALTTLSVNSSLTEVLAEGLERLREAERAGHPAE
                     VARCGLAVAEAHRRLGSTAEADRAWKASYRAARTAGDTGAMAWALWSGGTLARQRGAFP
                     LAWRLLGLAAELGERGGDVVVRGYSLAGLAETGRIQGDYAAVGRLHEQLLAEARKRGEA
                     RHTVWALEGIAQMHRNTGDFDRAYALFEEAAEIAERADDRRGHAWALRGLADILSARDG
                     DTGRALELLSRAEATCREMNLSSALAYNHKMRGNVCYRAGRYEEARDLYGLALSEFRTM
                     DEPRGEALSRLGLAKSLARLGRDPEETAAELAELALVLERSGLRHAREMVARAQAEFVP
                     EAEGAR"
     gene            complement(12212..12889)
                     /locus_tag="EV284_RS19975"
                     /old_locus_tag="EV284_4004"
     CDS             complement(12212..12889)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433207.1"
                     /locus_tag="EV284_RS19975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4004"
                     /product="AIM24 family protein"
                     /protein_id="WP_130335989.1"
                     /transl_table=11
                     /translation="MKGDLFSSEHMVRPAVEPGMATENGKCVRYVVDGEMLARQGAMIA
                     HRGSLQFERKGQGVGGMLKRAVTGEGLPLMSVRGRGEAWFAHEAQNCFIVEVEPGDQFT
                     VNGRNVLCFDASLSYEIKTVKGSGIAGGGLFNSVFSGHGRLGLMCEGNPLVIPVSEQAP
                     VYVDTDAVVGWTANLRTSLHRSQSIGSMLRGGSGEAVQLMLQGEGVVVVRPSEATPQKA
                     QQH"
     gene            complement(12962..14173)
                     /locus_tag="EV284_RS19980"
                     /old_locus_tag="EV284_4005"
     CDS             complement(12962..14173)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379768.1"
                     /locus_tag="EV284_RS19980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4005"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_130335991.1"
                     /transl_table=11
                     /translation="MVSATVGRSAVLGAVALALLVVPAQAATGEKGAAAALPAPDTAGV
                     DGALRTVRAQGAPAALARIDDQGRSHLISRGVADRGTGRAASTADRFRIGSVTKSFSSV
                     VLLQLADEGKLDLDAPVNRYLPKLLPSDAITVRHVLSHRSGLYDYTNDMFAQSVSGFEA
                     VRAKVFTYPQLVALSLKKPRVNAPGAAYSYSNTNFVVAGMLIEKLTGRPVKEAYQNRIM
                     GPLKLRDTFYVHPDTRIPGRFARGYLTPDRSGDPLVDATEQTVSWAQSAGAVISTAKDL
                     NTFYAALLGGKLLSAARLAQMERFTKVDATTAYGLGLRRRDLSCGVSVYGHTGAVQGYY
                     TYAFATKDGKRALTAVATTSNNGKVLNALAGALEPAFCGKSAKQRRGPALVERNADIAP
                     AAAR"
     gene            14488..16284
                     /locus_tag="EV284_RS19985"
                     /old_locus_tag="EV284_4006"
     CDS             14488..16284
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057077.1"
                     /locus_tag="EV284_RS19985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4006"
                     /product="M4 family metallopeptidase"
                     /protein_id="WP_130335993.1"
                     /transl_table=11
                     /translation="MRRPHIRSVAVAVAVATAATGLAGTAFARPAPSTAHKAAEGVRTA
                     ARTAPVVNAARAAAVAHADASGVGTGDELRVQDVMFDPEGGRHVRFQRAHSGMPVLGGD
                     LVVHLDRQLAYTGVTTAAGQSVQATASDARITPRQAAAKAADAAEGDAGTAELVVDARD
                     GETALAYRVTVTGSALDGASGTRTVVVDAVTGEVRSNTPDADEFLSPALRKALRARGET
                     VTPSTSAQSAPSLLAGITSGASGYPATARGTGKTLFAGSVPLTTTQTSRGHFQLKDPTR
                     YNTETRDAKGKYTESFGGGTAITGTANVWGNGSTSNRNSAAADAQYGITKTLDFYKKTF
                     NRSGIANNGKAARAMVHWGRGVGNAFWDPACGCMLYGDGDDMIKKPLVVLDVTGHELTH
                     GVVDATAKLEPTYVDRDGNQYGEPGSLNESLADIFGSNVEFFAANAKNPPNYLLGEKLG
                     LKQTFLRRLDHPSLDKLEGTVDYWSKATYDTEVHAGSGVSSHAYYLLAEGSGKKKIGGV
                     SYDSPTYDGGAVKGIGRTKATAIFYRALTRYMVSTTDFHDARMATLNAARDLYGATSTE
                     YRTVDRAWAAVNVKAANTPANH"
     gene            16430..17638
                     /locus_tag="EV284_RS19990"
                     /old_locus_tag="EV284_4007"
     CDS             16430..17638
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546056.1"
                     /locus_tag="EV284_RS19990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4007"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_130335995.1"
                     /transl_table=11
                     /translation="MSGRGRACRAAAVLLLTLTGQATPALGAAPPTGATVRGSGVVTWA
                     AGAQRVGQGADRAYRLIVKVSAGGSDLRVRLSNAYGDRPLTVDAAHAGLQAEGAALEPR
                     SNRALTFGGRHTVSVPPGGAVWSDPLPGRVPAGARLAVSLRTPHARGPATGHQLALTTS
                     YLGRDADPGGDDDTGWTSVTRSWWYLDAVSVRPAHPRAGAVVALGDSITDGWGSTPGLD
                     RRWPDDLARRLRADGTGVANAGISGNRVLTGDDGGESAGRRLERDVLSQPGVRTVILYE
                     GVNDLKARHGAGADEIVAGYREITERAHAAGLCVVGATLAPFKGWPQWDSDAEAVRREV
                     NHQVRTGGGFDAVADVDRALRDPRDPARLRPGLDSGDHLHPDDDGMRAIAGAVATPGRD
                     CRR"
     gene            complement(17646..17996)
                     /locus_tag="EV284_RS19995"
                     /old_locus_tag="EV284_4008"
     CDS             complement(17646..17996)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987898.1"
                     /locus_tag="EV284_RS19995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4008"
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="WP_130335997.1"
                     /transl_table=11
                     /translation="MSEDTAIDPSVPPSGTGCVECEKADSWWFHLRRCARCGHIGCCDD
                     SLGKHATAHYQETGHPVLRSFEPGETWFWNYATGELYESGPALAPPESHPADQPVPGPA
                     GRVPADWARLLS"
     gene            complement(17993..19462)
                     /locus_tag="EV284_RS33565"
                     /old_locus_tag="EV284_4009"
     CDS             complement(17993..19462)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607238.1"
                     /locus_tag="EV284_RS33565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_4009"
                     /product="ATP-binding protein"
                     /protein_id="WP_130335999.1"
                     /transl_table=11
                     /translation="MSGRQLPCSPGEIGSLFLFERLSPDQLGRLCREGRVELFEPGPVY
                     TEGDPATCFYVLIEGAVVLSRRIGAEDVEVSRTSQRGVYGGAMQAYLGDRVRQLYNNSM
                     RVTEPTRFFVLPAPVFAEIMRAWFPMAVHLLEGLFFGSKSTQAAIGQRERLLALGSLSA
                     GLTHELNNPAAAAVRATSALRERVARMRHKLSLIAEGPFSRDALASLIEMQERTAERVA
                     KAPALGPLEAADREDALADWLTDHGIEDGWRLAPVFVQAGLDADWLERVASMVDREILG
                     GAVGWLNYTIETELLMNEIEDATTRVSQLVDAAKQYTQLDRAPFRVVDVHELLDSTLLM
                     LSGKLGPHIEVVRAYDRTLPSLPGYPAELNQVWTNLIDNAVSAMTQDGGGGTLTVRTAR
                     EGDSALVEFADTGPGVPPEIREQIFDPFFTTKPVGAGTGLGLDISRRIVVGKHHGTLRL
                     ESAPGDTRFQVRLPLTADTTSGETDRAQEGA"
     misc_feature    complement(19728..19742)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.73"
                     /tool="antismash"
ORIGIN
        1 ccggcgagcc gggccagcgc gtcacgggtc gactcgtcct ccccggcgag cgggccgccc
       61 cggtagctcc ggggggcgtc cgcggtcggg gtccggcgca gggcggccac gaacgcgccc
      121 agctcctcgg ccagtcgctc cggctcctcc accgcgcccg cgaccgggtt ggcgccgtcc
      181 agccagcggt agaccgacca cggccaggga aagccgccgt ccggctcacc ggagcccacc
      241 ggttcgggga cggccaccgg caggccgggg cccaggcggg gcagccagtc ccgctcgtgc
      301 gtcacgtccg ccaccgcgcc cggatggcgg gggagccgta ccaccagttc cggccccagc
      361 cggaacatgg cgttctccgt gccggacgac ctcagccggg tcgccggcag gcccgcccac
      421 gcggggaacc gccgcgccac caggcgctcc accagcgggg cgtcgatgtc gagttcaccg
      481 tcgcgcatct tctgggtact caccggcccc atccaagggc cggagtgtcc ccgctgtcga
      541 ccgaaaaaga cctgaccacc ccgtccccga gcgtcccgcc cgccgagtgg ctagcgtgac
      601 gatccgggac gaaccgttca acacgagagg gccgaccatg gccgtgcaat ccgaaggaac
      661 gccctgctgg gccgacgcga tgttcaccga cctggagggc gccaagcggt tctacggcga
      721 cgtcctcggc tggaccttcg gcgaatcgtc gtccgagtac ggcaactaca cccaggcgta
      781 cgcggacggc aaggccgtcg cagccgtcgt cccgcccatg cccggccagg agggcacctc
      841 tcagtggtgt ctctacttcg cctcgccgga cgccgccgcc accgccgcca aggtcgagga
      901 gaacggcggc gaggtgctga tggagccgat gcgggtcggt gacttcggca cgatggcgat
      961 cgcccgcgaa cccagcggcg ccgtgttcgg catctggcag ggcggcgccc acgagggttt
     1021 cgaggcggtg gccaagcccg gcgcctactg ctgggcggag ctgtacaccc gcgaaccgga
     1081 gaaggcggac gcgttcctgt cggccgtctt cccgtaccgg atgaaggaca tggcggacgg
     1141 acacgtcgac ttccgcctct tcgacctggg cgccgatccg gtgctcggcc ggatgcggat
     1201 gggggaggag ttcccgccgg aggtcccctc ctacgtcaac gtctacttca ccgtcgacga
     1261 ctgcgacgcg gccgtggccc gcgccaccga gcggggtgcc gtgctccggt tcgggccgat
     1321 gaccagcccg ttcggccggt tcgccgccct caccgacccg cagggcgcga gcttctcggt
     1381 gatcgacgtc accacgacgg agggcggcat gccggagttc aaggacgcct gacccggccg
     1441 gtcggacccc gcgcgcggcc atggcatgat cgggcacatg cgtgaacgtg tggtggccgc
     1501 gtgcgacggg gcttcgaagg gaaaccccgg accggccgga tgggcgtggg tggtctccgg
     1561 cgacgacgag cggaacccgg cccgctggga gtcgggcccg ctcggccggg ccaccaacaa
     1621 cgtcgctgaa ctcaccgcgc tcgaacggct gttgaccgcc gtcgaggcgg acgtgccgct
     1681 ggaggtccgg atggactccc agtacgcgat gaaggccgtc accacctggc tgcccggctg
     1741 gaagcgcaac ggctggaaga ccgccgccgg caagccggtc gccaaccagg acctcgtggt
     1801 ccgtatcgac gcgctgctcg acggccgctc ggtcgagttc cggtacgtcc ccgcccacca
     1861 ggtcgacggt gacccgctga acgacttcgc cgaccgcgcc gccagccagg ccgccgtggt
     1921 gcaggaggcc gcgggcagcg cccacggctc gcccgagccg ccgccctcgc ccgacacccc
     1981 ggccccctcc gcccgccgca agtcgccccg gcgggccgcc ggtacctccg gttccgcctc
     2041 ctcgcggacc atcaaggcca agttcgccgg ccgctgcctc tgcggccgct cctacgccgc
     2101 cggcgagtcc atcgccaaga acgcgcaggg ctggggccac ccggagtgcc gtaccgccac
     2161 cggctgaccg gtcaccgccg gacgcctcgg ggtccggcca tgcccggacg ctgctaccct
     2221 gcgaggccga tcgactacgt ccggtgactt ccaggggtgt gcgtgtgaag gtcgtctgcg
     2281 tcgggggcgg gccagccggc ctgtacctcg ccatcctgct caagcggcag aacccgtccc
     2341 acgacatcac cgtccatgaa cgcgcccccg agggctccac gtacggctgg ggcgtcacct
     2401 actggcaggg cctgatggac cggctgcgcg cccacgaccc cgagtcggcg gacgccatcg
     2461 cccgcgactc ggtccactgg cacgaaggcg tcgcccatgt gcggaacctg atcacccgtc
     2521 agccgggcga ccagggtttt gggatcggcc gccaccgcct cctcgaactg ctcgccgaac
     2581 gcgcccggac cctgggcgta cggctggagt tcacgagcga gatcaccgaa cccccggccg
     2641 gggccgacct ggtggtggcg gccgacggca tcggcagcgc cctgcgcacc cggtacgcgg
     2701 acgcgttcgg cacccacgtc acccacggcc gcaaccactt cctctggctc ggcaccacca
     2761 aggtcttcga cgccttcacc ttcgccttcg tcgagaccgc gcacggctgg atctgggcct
     2821 acggctaccc ctacgacgcc gggcacagca cctgcgtcat cgagtgctca cccgagaccc
     2881 tcaccggcct cggcctgcac agcgccggcg acggcgacgg gctcgccctg ctggaggacc
     2941 tcttcgccgg actcctcgac ggccatcccc tcatcggccg cgcgtccgac ggcatcagcg
     3001 tcccctggct gaacttccgc accctcacca accgcacctg gcaccacgac ggcctcgtcc
     3061 tgctcggcga cgccgcccac accacccact actccatcgg cgcgggcacc accctcgccc
     3121 tggaggacgc catgtgcctg gccaccgccc tcggcgagca ccccgctctg cccgacgccc
     3181 tcgccgccta cgagaagcgc cgccgatccg agctgctgtc cgcgcagagc gccgcccgct
     3241 acagcgcccg ctggtacgag agcctgcccc gctacatcga actgcccccg caccggatgt
     3301 tcgccctgct cggccagcgc cactccccgc tgctgccgta cgtgccgccc cagctctact
     3361 acggcctcga caaagcggcc ggacagctcg aaccactgcg ccggctcaag cgctggctcg
     3421 gcccccgcgc cgcccgcgcc ctgcacgccg cgcgcactcc cggccgctcc cgctgacccc
     3481 ctccgccccg gctcaggcgc gggacaccgg ttggtgaata gccattcacc ggatagagtg
     3541 aatggctatt caccagtcag cacattcccc cgcaggagcg tcgatggacc cctccgcccc
     3601 catacccgag ccgtccggcg ccgtcacggc gccgccccgg ctgcgggagc gcctcaccat
     3661 ccccgtgctg gcgtcgggcg gcatcctcat ggcggtcatg cagactgtcg tcgtcccgct
     3721 cctgcctgac ctgccccggc tcaccggatc ctcgccggcc gccgtctcct ggatggtcac
     3781 cgccaccctg ctctccggcg ccgtgctcac cccggtcctc ggccgcgccg gtgacatgta
     3841 cggcaagcgc cgggtgctga cctccgcgct cgccctgatg accgtcggct cggtgatgtg
     3901 cgcgctgtcc tccgacatcg ccgtgctcat caccgcccgc gccctccagg gcgccgccgc
     3961 ctccgtcgtc ccgctgtcca tcagcatcct gcgcgacgaa ctcccgcccc agcggcgggg
     4021 gtcggcggtc gcgctgatga gttccaccgt cggcatcggc gccgccctcg gcctgccgct
     4081 ggccgcgctc gtcgtgcagt acgccgactg gcacaccatg ttctggctga ccagcgccct
     4141 gggcgcgctc ggggtcgcgg ccacctggtg ggcggtccgg gagtcgccgg tgcgcgagcc
     4201 cggccggttc gacgtggtcg gcgcgctggg cctggccgtc gcgctggtgt ccctgctgct
     4261 cgccgtctcc cagggcggcc agtggggctg gaccagcccc gccgtcctcg gcctgttcgc
     4321 cgccttcgcc gtggtgctcg ccctgtggtg gcgccagcaa ctgcggaccg cccggccgct
     4381 ggtggacctg cggctggtga cccggccgag ggtgggcctg tcccatgtgg cggccctgct
     4441 caccggcttc gcgttctacg ccaactcgct cgtcaccgcc cagctcgtcc aggcgcccgt
     4501 ggccagcggc tacgggctcg ggctgtccat cgtcgccacc ggactctgtc tgctgcccgg
     4561 cggcgtgacc atgctgctct tctcgccgct gtccgcccgg atctcagccc ggcgcgggcc
     4621 ccggatcacc ctcgccctcg gtgccgcggt catcgcctgc ggctacgcgc tgcgcatcgc
     4681 cgacagccgc gacctgtgga tgatcatcct cggggcgacc gtggtggcca ccggaaccac
     4741 cctcgcctac tccgcgctgc ccaccctgat cctgcgcgcg gtaccggccg cgcagaccgc
     4801 gtccgccaac ggagtcaacg tcctgatgcg gaccatcggc caggccgtct ccagcgccgc
     4861 cgtcaccgcc gtactggtcc accacaccag cctagtcggc gggcttccgg tgcccacgct
     4921 gcacgggtat ctgatggcct tcgccatggc cggcgccgtc gcgctcgccg cctgcgcggc
     4981 cgcactcacc atccccggag acggcgcccc cgacgacacc ccccgtgccg agggcgccac
     5041 cgcgggggct cgtgccctgg aggaggcgtg accaccgcgc cggcccccgc gcgccgggac
     5101 gccgaggcga ccaaggaggt catcctcagg gccgcccggc acctgctggc ccggcacgcg
     5161 cacgccgaca tcaccctcaa ggccgtcgcg gagcgggccg gggtcagccc gccgctggtg
     5221 gtgaagtact tcggcaacaa ggacgccctg ttcgcccgcg tcatgtcctt cgacaccgac
     5281 gccgacgacc tcctcgacgc ccccctcgcc ggcctcgggc ggcacatggt ccggcatgtc
     5341 ctggccagcc agcgcgaccg gggcgccgac ccgctgctgc gcatcgcctt cgccccgctg
     5401 cacggcgacc acggcgacat cctgcgcgcc aacttccgcg cccaggtcac cgaccgcctg
     5461 gccgcccggc tcaccggccc cgacgccggg ctgcgcgccg aactggccgt cgccgcgctc
     5521 atcggggtcg gcgtcatgta cggcatcgcc cgcggccccc acctgcgcga gagcggcccg
     5581 gacgaggtgg ccgaccgcta cgggccgctc gtccaggcgc agttgacgcc ctgacctaca
     5641 cctgagcgcc ggtcagcagg gactccgcgc agcggcggaa ggcggcgagc aggcggccgt
     5701 tgtcgcccgc gcgggtggcg agcgcgacat ggcacggctc gacaccgtgc agcgggatcg
     5761 cggccagatc cggtcgcgcc ggcattcccg gtgcggccgc agcgatcgcc aacgcctgcc
     5821 cggacgcgac cagttcgaac ttgtcctcga tgtccccgat gagcggcccg tccggcgcgg
     5881 gccgtccgtc cgggcgcggg tcgatccgcc agaacgcgtc ccacagcggg tccgggacgc
     5941 ggggcagggg ctcgtccgcg atgtcgtcca gcgtcaccga ctccttgccc gccagccggt
     6001 ggtcgagggg gaccaccacc acgcgcggct cgtcgtagag cacgctcacg cgcagcccgt
     6061 cggtggcaaa cggcagccgc gccaccagcg cgtccacccg gtggccgagc agcgcctcgc
     6121 gcgcctcgtg ccagcgcacg tgcgcggccc gcacgtccgc gtccgggaac ctgcgccgca
     6181 tctcccgcac ggcgggggtg accagcacgc cggtcgtgta gccgacggtg atccggcagg
     6241 gttcggcggc cgcccgcgcc tccgtcgccg cctcgtgcgc ggcccgcagc agtgccctgg
     6301 cgcggggcag gaagacctca ccggccccgg tgagccgggt gccctgcggg gtgcggtcca
     6361 gcagctcgac gccgagctcc cgctccagcc gccggacctg acggctgagc gagggctggg
     6421 tgatgtgcag ggcggtggcc gcgcggccga agtgccggtg ctcggcgacg accgtgaagt
     6481 accggaccag ccggagatcg agatccgcga agtcggacat gctctccagc gtacggccct
     6541 cgcaccagcg gtgatgcccg gaacgtattg cgccatgcgg aacaagcctt ggacgcgcgg
     6601 gttccgtccg gccgacgatg gaggcacttc caccgaccat cctaggagag cttcaccatg
     6661 cgtgttttcg tcaccggtgc caccgggttc atcggctccg cggtggtacc cgaactgatc
     6721 ggcgccgggc acgaggtcgt cggcctcgcc cggtcggacg acggcgccgc cgcactgaag
     6781 gaggcgggcg ccgaggtgtt ccggggcagc ctggacgacc ccgagggcct ggcccgcgcg
     6841 gcggacggcg ccgacggcgt gatccacctg gccttcatcc acgacttcgt cgacttcgac
     6901 gcggccggcg ccaccgacct gcgggccatc gaggcgatgg gcgccgtcct ggcgggctcc
     6961 ggccggccgc tcgtcgtcac ctccggcacc gccatcgccc cggcgggccg cctcgtcacc
     7021 gaggccgacg cgcccgcgcc gggcacccac cggaccccgt cggaggaggc cgccgtcgcg
     7081 ctggcggagc ggggcgtgcg cggatcggtg gtgcggctcg cgccctccgt gcacgggccg
     7141 ggcgatcagg gcttcgtgcc gcacctcatc cgcatcgccc gcgagaaggg cgtcgtcgcc
     7201 caggtcgggg acggtgccaa ccgctggccc ggtgtccacc ggctcgacgc cgcccggctg
     7261 ttccggctgg tgctggagca ggccccggcc ggtacccggt ggcacgcggc cgccgacgag
     7321 ggcgtgccgt tccgggagat cgcggaggtc atcggccgcc acctggaact gcccgtggtg
     7381 agcgtccccc cggagcgggc gggggagcac ttcgggttcc tcggccggtt cgccggggtg
     7441 gacaacccca cctccagcgc cacgacccgc gaacggctcg ggtggcggcc caccgggccc
     7501 ggactcctcg ccgatctgga ccagggccac tacttcgagt gaacgagcac agttgggccg
     7561 atcaagtgat cggtttgaaa gaacgtttgc gcggttaatt gcaccgcatg accgaggagg
     7621 caccgcacag ggggcgggac gagaccaggg aagagcgggc cgaccgcaag tggggcgaac
     7681 tgatccagga ggtacgtgtc gcgcagaccg gggtgcagat cctgttcggc ttcctgctga
     7741 cggtggtctt ccagcccaag tacggccagc tctccagcac cgaccagatc atctacatcg
     7801 tcaccgtggt cctgggggcc tgtgcgaccg gcgccctgat cggtccggtg tcactgcacc
     7861 gcctggtctc cgggcgccgg gtgaagccgg aggcggtacg gctcgcgggg aggctgacct
     7921 tcgtcggcct ggtgctgctg ctggccacca tgacctgctc gttgctgctg atcctccggg
     7981 tggccaccca cgacggctat gtgccgtggc tggtcggcgc cgtcgtctgc tggtacctgg
     8041 tctgctggta cggcctgccg atatgggccc gttggcggca ctccgacccc gaggaccgct
     8101 aacgcccggt acgggccgcg agggccgcca gcgagcggtc gtggacccgg ctgagcagca
     8161 gcagggagag cacggagccg accagggcgc agaacatgtc ccactgggtg tcccacacat
     8221 cgccctgggt cgcgaggaag gcgtcggctc cgtgcccgcc gatcacggcg gccagccact
     8281 ccagcagctc gaagtaggca ctgaaggcca ggcaggcgca caccgtcagc ggggccagcc
     8341 agcggctgcc gcgcagcggc gaggtacggg tgagcagttc ccggaccagg atcgccggga
     8401 cgaagccctg catcagatgc ccgaaccggt cgtacggatt gcggctcagg tgcagggcgt
     8461 cccgcaccca gtcgcccgcc ggcacctgcg cgtacgtgta gtggccgccg gccgccagca
     8521 ccagggcgtg cgcggcgagc agaccgcaca gcagcccggt gagcgggaag cgccgccaca
     8581 ggaccaccac caggggcagc ccgatcagca cccagaaggt ctccaggaac cacgtcgtgc
     8641 ggtcccgcgc gccccacgcg gagacggcca ggcccacggc cgccaccccc gcgaacacga
     8701 cgggcggcac cgtgcggcgg acggtcggcc gggcggggtg gactgcggtc acggcgggct
     8761 cctggggctg gcggtgtcgg tcgccccatc gtccggccgc cgggcccgcg ggggcatgag
     8821 taccggtact catgcccccg ccgcgcgtcc ctagggcctt actcccgcag cgccgcgcag
     8881 cgcggtgagg caggagccga tggcctgctg cagctcctcc aggcggcgga ccatgtcgtc
     8941 ctcgtagaag tccccggcgt cgtcgaaggt cagctcctcg aaggcgcggg tcgccttctg
     9001 gaggctgccg tagaacttgg tgcgccgctc ccgcacccgg agttcggggt cggcctcgac
     9061 ggtgtcggcg accagcgtct tcagcgtgtc gtacgcctcc accgcccact cgccggtgtc
     9121 ctcgtcgtcc tccagcccgt cgatcagcga cagatggcgc tcggcctcct cgcgcagttc
     9181 ggcgggggcc tcgacggtct gccccgccgg ggtcctgatc tgcccggact ccgcgatctg
     9241 ccggacgtaa ccgatcttgt cggcggcgcg gctctcggtg gcgacggcct tcttcagatc
     9301 gtcggtgcgc accacgtccc gcgccagctc ggcctggagc tccgggtcct ccctgatccg
     9361 gtccagcagg gcggagcggg ccgcgcgggc cgtggagggg tcggccagga tggcggcgcg
     9421 cagcgcggtc gggttctcgg ccacctccag cgccttggtc ggccggatgc cctcggcctc
     9481 ggcggcctcc gcgatggccg tgccgcgctc ggaggtggcg ctgctacggg cggagtagta
     9541 gctcaaccag gcgtcggagt cggggagttg gacgtcctgg cccggctcca gcgtctcgaa
     9601 ctgcgggacc aggccgtcgt cggcggcccg gtcccacgcc cggtagtagc gcatgacccg
     9661 ctccggggag cactccgcca gctccgcgaa ctccttcgcg gagaccttcg gggtctcccc
     9721 ggccgcctgt ccgcccggcc gcacactgcg cgccaccatc agcgcgaagg cccagccgcc
     9781 ggtgcgcgcg tacaccccga actcgcgggc gtcacgcgcc accaggtcgg acaggggtgc
     9841 cggagcggcg gcagtcgtct cggtggggtc gatggccagg gtcacggatg gctctcctgc
     9901 gtacgggggc tcggtggtgc ggccacccgg ccggccgggc gccgatggca gcctatatgg
     9961 cccgataggc gatgtttggc cgcagtcgcc acccgctccc tcagcgctcg cggtgggcga
    10021 gcgtgtccag cagggcgccc agctcggccg ccgcgcccgg ctccagcggc agcccgtcga
    10081 gcaccgtacg agcggcggcc atgtgggccc gagcctccgc cagggccgcg gcacggccgc
    10141 ccgccgcctc gatcagcgcc gccgcctccg ccgtacggtg cccgcgcagc aggccgggca
    10201 gccgggcgcc ctcgggggcg gcgagcgcgg ccagaaccgg gtacgtcctc ctgccctcgc
    10261 gcaggtcgcc gcccaccggc ttgccggtga cctcggcgcg gccccagatg cccagcacgt
    10321 cgtccgcgag ctggaaagcg acccccgcgt gccgcccggc ccggtccagc gccgcgacga
    10381 ccgggcgcgg agccccgccc agcagcgcgc cgagcgcgag cgaacagccc agcagcgcac
    10441 ccgtcttgcc ctccgccatc acccggtact cctcgggcgt caccgcttcc gggcccgtcc
    10501 agggccgtgc ggcgaacagc aggtcgtcgg cctgcccccg catgacgtcg gccagcgcgc
    10561 ccgagagcag ccgcacccca ccggggacat cggccagaac gccgacggcc agcgcgaaca
    10621 gggcgtcccc ggcgaggacg gcgggacccg tgccgtacgc cttccacagc gccgggcggc
    10681 cgcgccgggt ggcgtcgccg tccatgatgt cgtcgtgcag cagcgagaag gcgtgcacca
    10741 gctccaccgc gaccgcgccc ggcacccccg ccccggcatc cgcgccggcg gcccgcgccc
    10801 cgagcaccgc cagcgcctgc cgcaccccct tgccgccggc ctccgcgccc tcctgccagc
    10861 cgaaggagta ggcggccatc tcggccagcc acgggtgcag tccggtgacc gcctcccgca
    10921 acgcgggccg caccaacccg gctgactcgg agaggactcg cgtcgccggg atccgctccg
    10981 cgcggaacac cgccgtcacc gggcaccctc cgcctcgggc acgaactcgg cctgcgcccg
    11041 cgccaccatc tcccgcgcgt gccgcagccc cgagcgctcc agcaccaggg ccagctcggc
    11101 cagttcggcc gccgtctcct cagggtcccg gcccagccgg gccagggact tggccagacc
    11161 cagccgggac agggcctcgc cacggggttc gtccatcgtg cggaactcgg acagcgccag
    11221 gccgtacagg tcccgcgcct cctcgtaacg cccggcgcgg tagcagacgt tgccgcgcat
    11281 cttgtggttg taggcgagcg cgctggacag gttcatctcc cggcaggtcg cctccgcccg
    11341 cgacagcagc tccagggccc ggccggtgtc cccgtcccgc gccgagagga tgtccgcgag
    11401 cccgcgcagg gcccaggcgt ggccgcgccg gtcgtcggcg cgctcggcta tctccgccgc
    11461 ctcctcgaac agcgcgtacg cccggtcgaa gtcaccggtg ttgcggtgca tctgggcgat
    11521 gccctccagc gcccacaccg tgtgccgggc ctccccgcgc ttgcgggcct cggccagcag
    11581 ctgttcgtgc agccggccca cggccgcgta gtcgccctgg atgcggccgg tctccgcgag
    11641 ccccgccagc gagtaccccc ggacgaccac atcaccgccg cgctcgccga gttcggccgc
    11701 cagccccagc aaccgccagg ccagcgggaa cgccccccgc tgccgggcca gcgtgccgcc
    11761 gctccacagc gcccacgcca tcgccccggt gtccccggcc gtacgggcgg cgcggtaact
    11821 cgccttccac gcgcggtcgg cctccgcggt gctgccgagc cgccggtgtg cctcggccac
    11881 cgcgagaccg cagcgggcca cctccgcggg gtgcccggcc cgctcggcct cacgcagccg
    11941 ctccagcccc tcggcgagca cctcggtcag cgaggaattg acggacaggg tggtcagggc
    12001 gccctggtac tccggggcga aagccttgcc gtacatgtga acccttctat acaccacgtg
    12061 tatacgcggc gcgtatagca cgtgggcgaa tcgccccggt ccaccggggg ccggggcgtg
    12121 gtcggtcgtg gggcaagacg cgggcacggc gacgggggtt gcccgtgcga cgcggatcac
    12181 tccgagtggc gcggggaacc cccgtacgga atcagtgctg ttgggccttc tgcggggtgg
    12241 cctcgctggg ccggaccacc acgacgccct cgccctggag catcagctgg acggcttcgc
    12301 ccgaaccgcc gcgcagcatc gaccctatgg actgcgagcg gtgcagcgag gtgcgcaggt
    12361 tcgcggtcca gcccaccacg gcgtccgtgt cgacgtacac cggcgcctgc tcggagaccg
    12421 ggatcaccag cgggttgccc tcgcacatca ggccgagtct gccgtgcccg ctgaagacgc
    12481 tgttgaacag gccgccgccc gcgatgccgg agcccttcac ggtcttgatc tcataggaca
    12541 gcgaggcgtc gaaacacagc acgttgcggc cgttgacggt gaactggtcg ccgggctcca
    12601 cctcgacgat gaagcagttc tgggcctcgt gcgcgaacca cgcctcgccc cgcccgcgca
    12661 ccgacatcag cgggagcccc tcgccggtca ccgcgcgctt gagcatgccg cccacgccct
    12721 ggcccttgcg ctcgaactgg agactgcccc ggtgcgcgat catcgcgccc tggcgggcga
    12781 gcatctcacc gtccaccacg tacctgacgc acttgccgtt ctctgtcgcc atccccggct
    12841 cgacggccgg acggaccatg tgctcactgg aaaagaggtc acccttcatg agcgcatggt
    12901 gtcccggacg gacccgttcc gccaagatca cgcgagggac gggccgtccg ggacggggac
    12961 ttcagcgcgc cgcggccggg gcgatgtccg cgttgcgctc caccagcgcc gggccgcgcc
    13021 gctgcttggc gctcttgccg cagaacgccg gctccagggc gcccgccagc gcgttgagga
    13081 ccttgccgtt gttggaggtg gtggccaccg ccgtcagcgc ccgcttgccg tccttcgtgg
    13141 cgaacgcgta ggtgtagtag ccctgcaccg cgcccgtgtg cccgtacacc gacaccccgc
    13201 aggacaggtc ccggcggcgc aggccgaggc cgtacgcggt ggtcgcgtcg accttggtga
    13261 agcgctccat ctgggcgagc cgggcggcgg agagcagctt gccgccgagc agtgccgcgt
    13321 agaaggtgtt gaggtccttg gcggtcgaga tgacggcgcc cgcgctctgc gcccaggaca
    13381 cggtctgctc ggtcgcgtcg accagcgggt cgccggaccg gtccggggtc agatagccgc
    13441 gcgcgaaccg gccgggaatc ctggtgtcgg ggtggacgta gaaggtgtcc cgcagcttga
    13501 gcgggcccat gatccggttc tggtacgcct ccttgaccgg gcgtccggtg agcttctcga
    13561 tgagcatgcc cgcgaccacg aagttggtgt tggagtacga gtacgccgcg ccgggggcgt
    13621 tgacgcgcgg cttcttcagg gagagcgcga ccagttgggg gtaggtgaac accttcgcgc
    13681 gcacggcctc gaagccggag acgctctggg cgaacatgtc gttggtgtag tcgtacagcc
    13741 cgctgcggtg gctcagcacg tgccgcacgg tgatggcgtc gctcggcagc agtttcggca
    13801 ggtaccggtt caccggggcg tccaggtcca gcttgccctc gtcggccagt tggagcagga
    13861 cgaccgagga gaacgacttg gtcacgctgc cgatgcggaa ccggtctgcg gtgctggccg
    13921 cgcggccggt gccccggtcg gcgacacccc ggctgatcag atggctgcgg ccctggtcgt
    13981 cgatccgggc cagcgcggcc ggggcgccct gggcgcggac cgtgcgcagc gccccgtcca
    14041 cgcccgccgt gtcgggcgcg gggagcgcgg ccgccgcgcc cttctcgccg gtcgccgcct
    14101 gtgccgggac gacgaggagc gccagtgcca ccgccccgag taccgcgctc ctgcccaccg
    14161 ttgccgagac catcaggcca ccactcccgt ccacaccgcc gttgccgcga catgcgtcac
    14221 attccggcct ggaacctagg ggactgacgg cccgtctccc cagtcgacac acagtcggcc
    14281 atgcggcgat cactccgggg tgtcctttgg gtaaaggatt gtcccggaga gcagcgaaat
    14341 ggatcattcc ttctttacgt ggccatgact catccgaaag ggtgaaccct cggggcgcga
    14401 cccgcctgcc ccgatggccg cccaacggcg ccatgtgtca cgggggccgg cgcgtccggc
    14461 cgccgtcgaa cgaaggaaca acccctggtg cgcagaccgc acatacgcag tgtggccgtc
    14521 gccgtcgcgg tggcgacggc ggccacgggc ctggccggca cggccttcgc ccgccccgcg
    14581 ccctcgacgg cccacaaggc cgccgaaggc gtccggaccg ccgcacggac cgcccccgtg
    14641 gtgaacgccg cacgcgcggc cgccgtcgcc cacgccgacg cctccggggt cggcacgggt
    14701 gacgaactcc gggtgcagga cgtcatgttc gaccccgagg gcggccgtca cgtccgcttc
    14761 cagcgcgccc acagcggcat gccggtgctc ggcggcgacc tcgtcgtcca cctcgaccgg
    14821 cagctcgcct acacgggcgt gaccaccgcg gccggccagt ccgtacaggc caccgcctcc
    14881 gacgcccgga tcacccccag gcaggcagcc gccaaggcgg ccgacgccgc cgagggtgac
    14941 gccggcaccg ccgaactcgt cgtcgacgcg cgcgacggcg agaccgcgct cgcctaccgg
    15001 gtcaccgtca ccggcagtgc cctggacggc gcgagcggca cccgcacggt cgtcgtggac
    15061 gccgtcaccg gcgaagtccg cagcaacacc ccggacgcgg acgagttcct ctcgccggcg
    15121 ctccgcaagg cgctgcgggc gcgcggtgag acggtcaccc cgtccacctc cgcccagagc
    15181 gcgccgagcc tcctcgcggg catcacctcg ggcgcctcgg gctacccggc gaccgcgcgc
    15241 ggcaccggca agacgctgtt cgcgggcagc gttccgctga ccaccacgca gacctcgcgc
    15301 ggccacttcc agctcaagga ccccacccgg tacaacaccg agacgcggga cgccaagggc
    15361 aagtacaccg agtccttcgg cggcggcacc gcgatcacgg gtaccgccaa cgtctggggc
    15421 aacggcagca cgagcaaccg caacagcgcg gccgccgacg cccagtacgg catcaccaag
    15481 accctggact tctacaagaa gaccttcaac cggtccggca tcgccaacaa cggcaaggcc
    15541 gcccgcgcga tggtccactg gggccgcggg gtcggcaacg ccttctggga cccggcctgc
    15601 ggctgcatgc tgtacggcga cggcgacgac atgatcaaga agccgctggt cgtcctcgac
    15661 gtcaccggtc acgagctgac ccacggcgtg gtcgacgcca ccgccaagct ggagccgacc
    15721 tacgtcgacc gcgacggcaa ccagtacggc gaaccgggct cgctcaacga gtccctcgcc
    15781 gacatcttcg gctccaacgt ggagttcttc gccgccaacg cgaagaaccc gccgaactac
    15841 ctgctgggcg agaagctggg cctgaagcag acgttcctgc gccgcctcga ccacccctcc
    15901 ctggacaagc tggagggcac ggtcgactac tggtcgaagg ccacgtacga caccgaggtg
    15961 cacgccggtt ccggtgtctc ctcgcacgcg tactacctcc tggccgaggg cagcggcaag
    16021 aagaagatcg gcggtgtctc ctacgactcc ccgacctacg acggcggcgc cgtcaagggc
    16081 atcggccgca ccaaggccac ggccatcttc taccgtgccc tgacccgcta catggtctcc
    16141 accaccgact tccacgacgc ccggatggcg acgctgaacg cggccaggga cctgtacggc
    16201 gcgacgagca ccgagtaccg gaccgtcgac cgggcctggg ccgcggtcaa cgtcaaggcc
    16261 gccaacaccc cggcgaacca ctgacgcgca ccggctccga caaggccccg tcccgcaccc
    16321 gcgcggggcg gggcccttgt gcgcgggcgg acacggaacg taccgaggtg cggggcgcgg
    16381 gagacggcgg gaccatcaca ggcatgggcc agacgaagga accggccggg tgagcggccg
    16441 ggggagagcc tgccgggcgg ccgccgtcct gctgctcacc ctcaccggtc aggcgacgcc
    16501 cgccctgggg gccgccccgc cgaccggggc caccgtacgc ggctccggcg tggtcacctg
    16561 ggccgccggc gcccaacgcg tgggacaggg cgccgaccgg gcctaccggc tgatcgtcaa
    16621 ggtcagcgcg ggcgggagcg acctgcgcgt ccggctgtcc aacgcctacg gggaccgccc
    16681 cctcaccgtg gacgccgccc acgcgggcct ccaggccgag ggcgccgccc tcgagccgcg
    16741 cagcaaccgg gcgctcacct tcggcggcag gcacaccgtc tccgtccctc cgggcggggc
    16801 cgtctggagc gacccgctgc ccggccgcgt ccccgccggg gcccgcctcg ccgtcagcct
    16861 gcgcaccccg cacgcgcggg ggcccgccac cggccaccaa ctggccctca ccacctccta
    16921 cctcggccgg gacgccgacc cgggcggcga cgacgacacc ggctggacgt cggtcacccg
    16981 ctcctggtgg tacctcgacg ccgtctccgt acgccccgcg cacccccggg ccggcgcggt
    17041 cgtcgcgctc ggggactcca tcaccgacgg ctgggggtcc acgcccggcc tcgaccggcg
    17101 ctggcccgac gacctcgccc ggcggctgcg cgcggacggc acgggcgtgg ccaacgcggg
    17161 gatatccggc aaccgggtcc tcaccggcga cgacggcggc gagagcgccg ggcgccggct
    17221 ggagcgggac gtcctctccc agcccggcgt gcgcacggtg atcctctacg agggcgtcaa
    17281 cgacctcaag gcgcgccacg gcgccggcgc ggacgagatc gtcgcgggct accgggagat
    17341 caccgagagg gcccacgccg cgggcctgtg cgtggtcggc gccaccctcg cccccttcaa
    17401 gggctggccc cagtgggact ccgacgccga ggcggtgcgc cgggaggtca accaccaggt
    17461 gcgcaccggc gggggcttcg acgccgtcgc cgacgtcgac cgcgcgctgc gcgacccccg
    17521 cgaccccgcc cggctgcgcc ccggtctgga cagcggcgac cacctccacc ccgacgacga
    17581 cggcatgcgc gccatcgccg gggcggtcgc caccccgggc cgggactgcc ggcgctgacg
    17641 tgggttcagc tcagcaaccg ggcccagtcg gccgggaccc gtccggccgg gcccggcacc
    17701 ggctggtcgg ccgggtggct ctcgggcggg gccagggcgg ggccggactc gtacagctcc
    17761 cccgtggcgt agttccagaa ccaggtctcg cccggctcga agctgcgcag caccggatgg
    17821 ccggtctcct ggtagtgcgc ggtggcgtgc ttgcccagcg agtcgtcaca gcacccgatg
    17881 tgcccgcacc gcgcgcagcg gcgcagatgg aaccaccagc tgtccgcctt ctcgcactcc
    17941 acgcaccccg taccgctcgg cggcacggac gggtcgatcg cggtgtcctc actcatgcgc
    18001 cctcctgggc tctgtcggtt tcgccggacg tggtgtccgc ggtcagcggg aggcggacct
    18061 ggaagcgggt gtcgccgggg gccgactcca gccgaagggt gccgtggtgc ttgccgacga
    18121 cgatccgccg cgagatgtcc agcccgagac cggtgcccgc gccgaccggc ttggtggtga
    18181 agaacgggtc gaagatctgc tcccggatct ccggcggcac accgggcccg gtgtccgcga
    18241 actccaccag cgccgagtcg ccctcccgcg ccgtccgcac ggtcagcgtg cccccgccgc
    18301 cgtcctgcgt catcgcggac accgcgttgt cgatcaggtt ggtccacacc tggttcagct
    18361 ccgccggata gcccggcagc gagggcagcg tgcggtcgta ggcgcgcacc acctcgatgt
    18421 gcgggccgag cttgccggac agcatcagca gggtgctgtc gaggagttcg tgcacgtcga
    18481 ccacgcggaa cggcgcccgg tcgagctggg tgtactgctt ggcggcgtcc accagttggg
    18541 agacgcgggt cgtggcgtcc tcgatctcgt tcatcaacag ctcggtctcg atggtgtagt
    18601 tgagccagcc caccgcgccg cccaggatct cccggtccac catggaggcc acccgctcca
    18661 gccagtcggc gtccagcccg gcctgcacga acaccggcgc gagccgccag ccgtcctcga
    18721 tgccgtggtc ggtcagccag tcggcgagcg cgtcctcgcg gtcggccgcc tccagcggac
    18781 cgagcgccgg tgccttggcg acccgttcgg cggtgcgctc ctgcatctcg atcaggctcg
    18841 ccagcgcgtc acgggagaac gggccctcag cgatcagcga cagcttgtgc cgcatccgcg
    18901 ccacccgctc ccgcagggcc gaggtggccc gcaccgcggc cgccgccggg ttgttcagct
    18961 catgggtgag ccccgccgac aacgagccca gggccagcag ccgttcgcgc tggccgatgg
    19021 ccgcctgcgt gctcttggag ccgaagaaca gcccctccag cagatgtacc gccatcggga
    19081 accaggcccg catgatctcc gcgaacaccg gtgcgggcag cacgaagaac cgggtgggct
    19141 ccgtcacccg catcgagttg ttgtacagct gccgcacccg gtccccgaga tacgcctgca
    19201 tcgcgccgcc gtacaccccg cgctgggagg tccgggacac ctccacgtcc tcggcgccga
    19261 tccgccgcga gaggacgacg gcgccctcga tcagcacgta gaagcaggtc gccggatcgc
    19321 cctcggtgta caccgggccc ggttcgaaca gctccacccg gccctcgcgg cacaaccgcc
    19381 ccagctgatc gggcgacagc cgctcgaaga ggaacaggct gccgatctcg cccgggctgc
    19441 acggcagctg gcgcccgctc acgactgctc cagataacgg tggacgagca tcacggccat
    19501 ggctccctct cctacggcgg acgcgacccg cttggccgac tctgcgcgcg cgtcgcccgc
    19561 cacgaacacg cccggcacac tggtctccag gtggtacggc ggccgctcca gctcccagtc
    19621 cgccggcagc tgcccgtcca cggtcaggtc cgggccggcc aggatgaacc cccgcccgtc
    19681 ccggcgcacg gtcccgtcca gccagccggt cagcggggcg gcgccgatga acacgaacat
    19741 ccactgcgcc tcgacctggt cggcctcacc cgtgtccacc cggcgcaggg tcagccgctc
    19801 cagccggtcc gagccgtgcg ccgcctccac ctccgtgcgg gcgcgcaccg cgacgttcgg
    19861 cgcctcggtg atctgctgga ccaggtagtg cgacatggac gctgccaggt cggccccgcg
    19921 caccagcagc gtcaccgact tggcgaaccg ggacaggtac atcgccgcct gcccggccga
    19981 gttggcgccg cccacgacga acacgtcgtg cccctggcag gacggcgcct cggtcagcgc
    20041 cgacccgtag aacaccccgc agccggtcag ccccgcgcag ccgtcggcgg tcagccgccg
    20101 gtactgcacc ccggtcgcca ggatcacact gtgcgcgccg atcgcggagc cgtccgcgaa
    20161 ccgcacgatc cgcgccgcgc cgttgacctc gagtcccgtc acctcgcgcg ccgtgaggat
    20221 ctccgcgccg aaccgggtcg cctggcggcg ggcgcggtcg gtgagctggg cgccggagac
    20281 gccgtcgggg aagccgaggt agttctcgat ccgggagctc tgcccggcct ggccgcccgt
    20341 cgccgaacgc tccaccagca ccgtgcgcag cccctcggag gccccgtaca ccgccgagcc
    20401 gagcccggcc gggccgccgc cgatcaccac caggtcgtag aagtcggccg tcggcgtcgt
    20461 cgccagcccg acccgcgcgg ccagctccgg ggcctccggc tcgaccagca cccggccgtc
    20521 gggggtgatc accagcggca gccgcagccc gtcctgcccg gccgccgcca gcagccgccg
    20581 cccctcgggg tcctccaccg agtaccagcg gtaggggacc tggttgcggg ccaggaactc
    20641 gcgcacctcc gaggaccgag ccgaccagcg gtggcccacc accttgatga acggcaccgg
    20701 ccggtggtcc ccggcccgcc aggcggccag caggtcgtcc accaccgggt acagcttctc
    20761 ctccggcggg tcccagggct tgagcagata gtggtccagg tccaccacgt tgatcgcgtc
    20821 gatggccgcc ccggtgtccg cgtacgccgt cagcagcacc cgccgcgcac ccggaaagac
    20881 gtccatcgcg cgctccagga actcgacgcc gttcatcacc ggcatccggt agtccgccag
    20941 cagcaccgcc acggagtcgc cgcgcagctt cagctcgcgc agcgcctcca
//
