LOCUS       NZ_SHKU01000003        10483 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. BK022 Ga0307664_103, whole genome shotgun sequence.
ACCESSION   NZ_SHKU01000003
VERSION     NZ_SHKU01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. BK022
  ORGANISM  Streptomyces sp. BK022
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1080808)
  AUTHORS   Kalluri,U.
  TITLE     Understanding the reciprocal impacts of modified plant cell wall and
            associated microbiome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1080808)
  AUTHORS   Kalluri,U., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 215.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004217355.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 02:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,482
            CDSs (total)                      :: 6,401
            Genes (coding)                    :: 6,280
            CDSs (with protein)               :: 6,280
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 49 of 121
            Pseudo Genes (incomplete)         :: 92 of 121
            Pseudo Genes (internal stop)      :: 16 of 121
            Pseudo Genes (multiple problems)  :: 29 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:05:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 281204
            Orig. end                         :: 291687
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SHKU01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10483
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10483
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10483
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5483](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5483
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(319..1680)
                     /locus_tag="EV284_RS12765"
                     /old_locus_tag="EV284_2551"
     CDS             complement(319..1680)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /GO_process="GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132932.1"
                     /locus_tag="EV284_RS12765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2551"
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="WP_130334667.1"
                     /sec_met_domain="Bac_transf (E-value: 2.7e-62, bitscore:
                     205.3, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDSGYAVRPAQGFRFPARRPHSPTVSPLPLLTADLAAAVAGALVL
                     DAAVRGPLVVAALVTGALLLRPRPARAVAGVLDELPSVCGRIAVVWLALGTLTAATEPG
                     RALPLSALAVGWVAHVVVACAGRTAVHRRRRALRLRLPSTALVIGPAATAQRVAAALLR
                     QPECGVRPVGVVTDHPDGSAGLPVLTCGEQVERALIQNGVGTVLTVDPAVRTEKGPLLR
                     ALAESGCAVWEVDADCPAYEMREQLAGFSVRRLDLGARRRGSVGKRLLDLVVSGALLVL
                     AAPLLLACALVLRLSEGPGVVFRQERIGKDGRPFTLLKFRTHRPVDERESATRWSVADE
                     RRMNPFCRFLRRTSLDELLQLWNVFRGDMSLVGPRPERPYFVTQFSQCYPGYASRHRMR
                     VGITGLAQVSGLRGDTSIEDRARYDNAYIDNWSLWQDVCILLRTAAAVVRSTGS"
     misc_feature    1701..1715
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.96"
                     /tool="antismash"
     gene            complement(1719..2879)
                     /locus_tag="EV284_RS12770"
                     /old_locus_tag="EV284_2552"
     CDS             complement(1719..2879)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325465.1"
                     /locus_tag="EV284_RS12770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2552"
                     /product="glycosyltransferase"
                     /protein_id="WP_130333440.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 6.3e-32,
                     bitscore: 106.4, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHQPVSDARERVLHVSQPVDGGVARVVLDLARAQLAAGVRVVIAC
                     PGGRLATEAQEIGAEVRLWRATRSPGPALPGEVRRFARLVGEVRPALVHAHSAKAGLAA
                     RLALRGRVPTVFQPHAWSFEAVGGVTAALALRWERFAARWAERVVCVSEAERETGVRAG
                     VRAAYAVVRNGIDLERFSPAPAQAARAGLPGLGAGPLVVCVGRLCPQKGQDVLLAAWER
                     VLTVLPEARLVLVGDGPGREALGALAGARVRFAGEVADVLPWYRAADLVVLPSRWEGMA
                     LAPLEALACARPVVLSDVDGARESLPPALAGRCLVPPEDPDALAAAVTGLLCDAPLRAD
                     LGAQGRRHVLTTHDVRVTAERIADLYRDLLGTLPSVSTERRESTHP"
     gene            3449..3688
                     /gene="chpG"
                     /locus_tag="EV284_RS12780"
                     /old_locus_tag="EV284_2553"
     CDS             3449..3688
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="EV284_RS12780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2553"
                     /product="chaplin ChpG"
                     /protein_id="WP_030813959.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSAAVAAVAGGAGIAAADSGAQAVAANSPGVLSGN
                     VVQIPVHVPVNVCGNTIDVIGLLNPAFGNTCVND"
     gene            3900..4187
                     /locus_tag="EV284_RS12785"
                     /old_locus_tag="EV284_2554"
     CDS             3900..4187
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136064.1"
                     /locus_tag="EV284_RS12785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2554"
                     /product="hypothetical protein"
                     /protein_id="WP_086696322.1"
                     /transl_table=11
                     /translation="MKSLKAAAVLAGSIALTGVAAPAFAYSGDLTPSSLNGAVDQVAKG
                     GLKVRDVMPLQHQSDALNTENKDSVLSTVKGATTALNTHNPLLGGLPLQS"
     misc_feature    4278..4293
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.48"
                     /tool="antismash"
     gene            4412..4900
                     /locus_tag="EV284_RS12790"
                     /old_locus_tag="EV284_2555"
     CDS             4412..4900
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378444.1"
                     /locus_tag="EV284_RS12790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2555"
                     /product="DUF5949 family protein"
                     /protein_id="WP_130333442.1"
                     /transl_table=11
                     /translation="MTSTTSEAFRAADLGTLVVLAWSGEAPDGGDMPYLLAYSLGDAEG
                     GPRATSAAVTQLLEMNGLTIGTVIDGATRPSLPVSLLVEAGQAVVRMPQLVAQAGAPPE
                     WLDAVARRGHAYLVFTTRAWPEGRPGVTVDPAALAAFAGADETLMTAAHVMLPARSLR"
     gene            5001..5483
                     /locus_tag="EV284_RS12795"
                     /old_locus_tag="EV284_2556"
     CDS             5001..5483
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="EV284_RS12795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2556"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_130333444.1"
                     /sec_met_domain="melC (E-value: 3.9e-47, bitscore: 155.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVGAGGVAVGAEPQVREGKPSRREVTRGLLATVAALGLAPVVAA
                     SRPLPPAGGAEDRAFDETYRGHRIQGFLVPAAARRADEGEWQITVDGRPLHLMRRADGT
                     WMSMVDHYTSYATPLEAARAAVDEIGPGQRLRDTAPGPVGGEHAGHGMGGMHGVRA"
     gene            5467..6333
                     /locus_tag="EV284_RS12800"
                     /old_locus_tag="EV284_2557"
     CDS             5467..6333
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046795.1"
                     /locus_tag="EV284_RS12800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2557"
                     /product="tyrosinase family protein"
                     /protein_id="WP_207229016.1"
                     /transl_table=11
                     /translation="MAYVRKNAATLTASEKRRFVNALLEVKRRGEYDAFVRTHIDYYTA
                     DGEKGLRAAHMAPSFLPWHRRFLLELERALRRVDSSVTVPYWDWTRDRGTTSAPWTKDL
                     LGGNGRKSDRQVTTGPFAYAAGHWTIKEGITDGKYLTRDLGRAAAPIQLPTRSELQWAL
                     DDPAYDVSPWDSTVTRGFRNKLEGWGTGGGSASWHNHNRVHRWVGGVMLGGASVNDPVF
                     WLHHAFLDYQWYRWQQAHKNHRYLPAAQPGPGDKQRGRVVARLDKMPPWNVTPDSLEDV
                     SGIYRYA"
     gene            complement(6424..6717)
                     /locus_tag="EV284_RS12805"
                     /old_locus_tag="EV284_2558"
     CDS             complement(6424..6717)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545038.1"
                     /locus_tag="EV284_RS12805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2558"
                     /product="chaplin"
                     /protein_id="WP_207229017.1"
                     /transl_table=11
                     /translation="MSRIAKAAVVALGTGAVMVGGAGLALADADAGAAAVGSPGVGSGN
                     VVQVPVNIPVNLCGNTINVVGLLNPAFGNTCANVGEPEPVQEVPEGDYGYGN"
     misc_feature    complement(6701..6715)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            complement(6905..7288)
                     /locus_tag="EV284_RS12810"
                     /old_locus_tag="EV284_2559"
     CDS             complement(6905..7288)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850928.1"
                     /locus_tag="EV284_RS12810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2559"
                     /product="DoxX family protein"
                     /protein_id="WP_130333448.1"
                     /transl_table=11
                     /translation="MDLALWISAWALAVVALVGGVSKTFVPKEKLAAVHGGGWTEGVGA
                     RSVKALGVLELLAAVGLVLPALLDRVPVLVPVTAACWVLLMIGAMATHGRRREFGFVAL
                     NLAYLALAAFIAWGRFGPESFTG"
     misc_feature    complement(7606..7620)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.76"
                     /tool="antismash"
     misc_feature    complement(7720..7735)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     gene            7857..8477
                     /locus_tag="EV284_RS12815"
                     /old_locus_tag="EV284_2561"
     CDS             7857..8477
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004978949.1"
                     /locus_tag="EV284_RS12815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2561"
                     /product="DUF998 domain-containing protein"
                     /protein_id="WP_242625696.1"
                     /transl_table=11
                     /translation="MDGAAVRRRLAGGMAAGPVFLAVGAVQGLARDGFDFSRNALSQLA
                     LGQAGWLQTVNFLLTGAMLVMGAVGLRQVLGGGTGGTWAPLLVSVFGVSFWAAAAFPAD
                     AGAGFPVGATDGTVVSEHGVVHMVAGMVGYLALCAAFVVLAGSLAARGHRRWAVTSRLV
                     PVAVLAGFVASAASVLAFTAGAGLGLLWLTAVTARLATAPVDA"
     gene            8534..8740
                     /locus_tag="EV284_RS12820"
                     /old_locus_tag="EV284_2562"
     CDS             8534..8740
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004978947.1"
                     /locus_tag="EV284_RS12820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_2562"
                     /product="DUF4287 domain-containing protein"
                     /protein_id="WP_130333450.1"
                     /transl_table=11
                     /translation="MATKGPASYFPSIEKKYGLPITEWKALIRTSPLTKHMELVNWLKS
                     EHGLGHGHANALVAHTLTEDAGK"
     gene            complement(<8779..>9003)
                     /locus_tag="EV284_RS33230"
                     /old_locus_tag="EV284_2563"
                     /pseudo=""
     CDS             complement(<8779..9003)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018522249.1"
                     /locus_tag="EV284_RS33230"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="EV284_2563"
                     /product="RNA polymerase subunit sigma-70"
                     /pseudo=""
                     /transl_table=11
                     /translation="MQAWLTGPAIGCRGSRLVPTAANGSPAFGQYRPHPDGTGYVPWAL
                     QVVEFSGDRISAVTAFRDTERLFPLFGLPG"
     gene            9144..10316
                     /locus_tag="EV284_RS12830"
                     /old_locus_tag="EV284_2564"
     CDS             9144..10316
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EV284_RS12830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EV284_2564"
                     /product="hypothetical protein"
                     /protein_id="WP_130333453.1"
                     /transl_table=11
                     /translation="MTGAVLGGVLVSGVPVTSGPPSADVRALSAGSGHVLREEGRGHDE
                     WLTIDRLERDGGDLPREGTTFEVRAGFEHKGERVMRKRAFSISVSERLSFATEYSNCEY
                     GEDVPTSPVNRSFRKAVCWIDARIEPGESVDLAPIPLKVDRWANDETVSVADAGVREQW
                     AASGVVWRNRHWGRGGKLTLAKRAGDVPPAARVKAAVDMSSGGLDVNVDSSWDIEVTGA
                     ALNGRRGEADTADLAMDFHGADVQAQRDPENSVPFARVRVQFPRGVTVLRSPKQCGNRV
                     TKRPYYQCEYNLYTNSYDAPMLRDGYHEAFPFRLRIDDPSELTGGSISLDAPPEVLQDD
                     ADHANSTARITVEAVGGPDRTAWIVAAGAGTLALTGILAAVLLRARRRAD"
ORIGIN
        1 caggacgaag acctggaggt agcggccgag tccggcgacg ccggcgcccg gggaggcggc
       61 gcccatcgcg gcgagcgccg caccggccac cggcagcccc agcaccagcg cggccctggc
      121 ggacagcggg cgggcccgct cccgcagcag ccgcaccagg caggcgccga cgacgagcgc
      181 ggacgccgcg tcggccgggc ccgcgccgcc cccctcgctg gaggagaggg gcagcgcgag
      241 caggaagagc acggcgagca ccggcagcac cggggaccag tgccgggggc ggccgagcgg
      301 cagcgcgagg ctcatccgtc agctcccggt ggaccgcacg acggcggccg cggtgcgcag
      361 caggatgcag acgtcctgcc acagcgacca gttgtcgatg tacgcgttgt cgtagcgcgc
      421 ccggtcctcg atcgaggtgt ccccgcgcaa cccgctgacc tgcgcgagcc cggtgatgcc
      481 cacccgcatc cggtgccggg acgcgtagcc ggggtagcac tggctgaact gcgtgacgaa
      541 gtaagggcgt tcggggcgcg ggccgaccag gctcatgtcg ccgcggaaga cgttccagag
      601 ctggagcagc tcgtccagcg aggtccggcg caggaaccgg cagaacgggt tcatccgccg
      661 ctcgtccgcc acgctccacc gggtcgccga ctcccgctcg tccaccggac ggtgggtgcg
      721 gaacttcagc agggtgaagg ggcgcccgtc cttgccgatg cgctcctgcc ggaacaccac
      781 gcccggaccc tcgctgagcc gcagcaccag cgcgcaggcc agcagcagcg gcgcggccag
      841 caccagcagg gcccccgaca cgaccaggtc cagcagccgc ttgccgacgc tgccgcgccg
      901 ccgggcgccg agatcgagcc gccgtacgga gaacccggcg agctgctccc gcatctcgta
      961 cgccgggcag tccgcgtcca cctcccacac cgcgcagccc gactcggcga gcgcccgcag
     1021 cagcgggccc ttctcggtgc gtacggcggg gtcgacggtg aggacggtgc cgacgccgtt
     1081 ctggatgagc gcccgctcga cctgctcgcc gcaggtcagc accgggagcc cggcgctgcc
     1141 gtccgggtgg tcggtgacca cgccgacggg acgtaccccg cactccggct gccgcagcag
     1201 cgcggcggcg acccgctggg cggtcgcggc ggggccgatc accagcgcgg tgctcggcag
     1261 ccgcagccgc agcgcccggc gccgccggtg cacggccgtc cggccggcgc aggcgaccac
     1321 cacgtgcgcc acccagccca cggccagcgc ggacaggggc agggcgcgcc cgggttccgt
     1381 ggcggcggtc agcgtgccga gcgccagcca gaccacggcg atccgcccgc agacggaggg
     1441 gagttcgtcg agcacaccgg ccaccgcgcg cgccgggcgc ggccgcagca gcagggcgcc
     1501 ggtgaccagc gcggccacga cgagcggccc gcgcacggcc gcgtccagta cgagcgcgcc
     1561 cgccacggcg gcggcgaggt cggcggtgag cagcggcagc ggggagacgg tgggggagtg
     1621 cgggcgccgg gccgggaacc ggaagccctg cgcgggccgt acggcgtacc cggagtccat
     1681 gccggaggcg ctgggcgagg ggacgatgct ttccgcggtc acgggtgggt cgactccctg
     1741 cgctcggtgg acacggacgg cagggtgccg aggaggtcgc ggtagaggtc cgcgatccgc
     1801 tcggcggtca cccgtacgtc gtgcgtggtc aggacgtgcc ggcggccctg ggcgccgagg
     1861 tccgcgcgca gcggcgcgtc gcacagcagc ccggtcacgg cggcggccag cgcgtccggg
     1921 tcctccggcg gcaccaggca gcgtccggcg agcgcgggcg gcaggctctc gcgggcgccg
     1981 tccacgtcac tgagcaccac gggccgggca caggccagcg cctccagcgg ggccagcgcc
     2041 atgccctccc agcgcgaggg cagcaccacc aggtcggcgg cccggtacca gggcagtacg
     2101 tcggcgacct cacccgcgaa ccgcacgcgc gctcccgcca gtgcgcccag cgcctcccgg
     2161 ccgggtccgt cgccgaccag caccagccgg gcctccggca gcacggtcag cacccgctcc
     2221 cacgcggcca gcagcacgtc ctggcccttc tgcgggcaca gccgcccgac acacaccacc
     2281 agcggtccgg ccccgagccc gggcagcccg gcgcgggccg cctgggcggg cgcgggggag
     2341 aaccgttcga ggtcgatgcc gttgcgcacc acggcgtacg cggcgcgcac cccggcgcgt
     2401 acaccggtct cacgctccgc ctcactgacg cacaccaccc gctcggccca gcgcgcggcg
     2461 aaccgctccc agcgcagggc gagcgcggcg gtgaccccgc cgacggcctc gaaggaccag
     2521 gcgtgcggct ggaagacggt cgggacgcgc ccgcgcaggg cgaggcgcgc ggccagaccg
     2581 gccttggcgc tgtgcgcgtg caccagtgcg ggccgtacct ccccgacgag gcgggcgaac
     2641 cgccgtacct cgccgggcag cgcgggaccc ggtgaccggg tggcccgcca gagccgcacc
     2701 tcggcgccta tctcctgtgc ctccgtggcc agtcggccgc ctgggcaggc gatcacgacg
     2761 cgcacgcccg ccgcgagctg ggcccgcgcc aggtcgagca cgacccgcgc gactccgccg
     2821 tcgacgggct gcgaaacatg cagcacccgt tctcgggcgt cggaaactgg ctggtgcatg
     2881 ggcggggtac ctcgctcaca gggcgctcgg atctggtcgg gaccgcgcct cgctgccggg
     2941 cgtcctccgc ggtgaagagc acaccggccc tcgccgcgtc ccggtgggga acgagcaggt
     3001 ggcccggcgg gtcaccgccg cgcccgcgcg acgcgcccga gatcggccac cctcggtccc
     3061 gcgtccggac ggcctggcgt agccggcctg gcgccggacc cgtacgcgcc ctgccgcgcc
     3121 ggtcgtgcgc tccggtacgt cgtccggtgg acccgcgccc ggccacttcc gtgcgcccgg
     3181 cgtacgacgg cgcggcacgc tgcctccctg aagcgtgagc cggttgcccg ccacgcacgg
     3241 ggtccggacc tggtacggag gagagggccg cgactctagt gagcgcgaag gcccgagcgg
     3301 cgcaacgacg cgagcgggcc gcccacgcgg ccgcggttct cgcggagatc acccctttgg
     3361 cggcacaacc cctcgcggcg gctcgcgttg acactgcgtc gggcagcccg cccggttgtg
     3421 tgtctcgaat tccaaggagt acctctccat gtcgcgtatc gcgaagggtc tggtcctgac
     3481 ctccgccgcc gtggcggccg tcgccggcgg tgccggtatc gccgctgccg actccggcgc
     3541 gcaggccgtc gccgcgaact cgccgggcgt cctctcgggc aacgtcgtcc agatcccggt
     3601 ccacgtgccg gtcaacgtct gcggcaacac catcgacgtc atcggcctgc tgaacccggc
     3661 cttcggcaac acctgcgtca acgactgagg tcgtccgacg cacacaccgg cccggccgtc
     3721 cgcccgcgcg tacagcgtga gcggacggcc gggccgctgt cgtgtgtcct cgcccccgct
     3781 agcgaggctg gggaatctca tatgtcttgt gcacacaggg tttccgcccc ggccgtgact
     3841 cgttgggcat ggcgtcggac atccctgggg cgacgcgagt tgccgaggag aggaacatga
     3901 tgaagtccct gaaggccgcg gccgtcctgg ccggttccat cgccctcacc ggcgtcgccg
     3961 cgcccgcgtt cgcctactcc ggcgacctga cgcccagcag cctgaacggc gcggtggacc
     4021 aggtcgccaa gggcggactg aaggtgcgtg acgtgatgcc gctgcagcac cagtcggacg
     4081 cgctgaacac cgagaacaag gactccgtcc tcagcaccgt caagggcgcc accaccgccc
     4141 tgaacacgca caacccgctg ctcggcggac tgccgctgca gagctgagcc accgtggagg
     4201 gagggccgga ccgcgccgcg cggaccggcc ctccctctcg tgtccgcggc ggcgccccgc
     4261 ttgtgtccga ggaagcgtcc cctgcgtgtt cgaggtggcg tcccccggtt tcgaggcccc
     4321 ttcccccgat cgtgtggagc acccccctgc cgtcccccgg acgagtgagc ggctccccgg
     4381 caggccccgc gcccgtggat agggtcgcgc ggtgacctca accacaagcg aagccttccg
     4441 cgccgccgac ctcggcaccc tcgtcgtcct ggcctggagc ggcgaggccc ccgacggtgg
     4501 cgacatgccg tatctgctgg cgtactccct cggggacgcc gagggcggac cgcgcgccac
     4561 ctcggccgcc gtcacccagc tcctggagat gaacggcctg accatcggca ccgtcatcga
     4621 cggcgccacc cggcccagcc tgccggtctc gctgctcgtc gaggccggcc aggccgtcgt
     4681 ccggatgccc cagctcgtcg cccaggccgg cgcgccgccg gagtggctgg acgccgtcgc
     4741 ccggcgcgga cacgcctacc tggtcttcac cacccgcgcc tggccggagg gccgccccgg
     4801 ggtgaccgtc gaccccgccg cgctggccgc cttcgccggt gccgacgaga cgctgatgac
     4861 cgccgcccac gtcatgctcc cggcgcgcag cctgcgctga cgcccggtcg cctacccgga
     4921 tggccgctgt gcggtgactc cgggcgcccc aacgcggtta ccgctgtgga caagccgaac
     4981 tgcggacgag ggagtgagcg atggtcgtcg gtgccggtgg tgtggccgtg ggggcggaac
     5041 cgcaggtcag agagggcaaa ccgtcgcggc gggaggtgac ccgcggcctg ctggccacgg
     5101 tggccgccct ggggctggcc ccggtggtcg ctgcgtcccg tccgctgccc ccggccgggg
     5161 gcgcggagga cagggcgttc gacgagacct accggggcca ccgcatccag ggcttcctgg
     5221 tcccggccgc cgcgcgccgg gcggacgagg gcgagtggca gatcaccgtc gacggccgcc
     5281 cgctgcacct gatgcgccgc gccgacggca cctggatgag catggtcgac cactacacct
     5341 cgtacgccac ccccctggag gccgcccgtg cggccgtgga cgagatcggc cccggccagc
     5401 ggctgcgcga cacggctcca ggtccggtcg gcggcgagca cgcgggacac gggatggggg
     5461 gcatgcatgg cgtacgtgcg taagaacgcc gccacgctca ccgcgagcga gaagcggcgc
     5521 ttcgtcaacg ctctgctgga ggtcaaacgg cggggcgagt acgacgcgtt cgtgcggacg
     5581 cacatcgact actacacggc ggacggcgag aagggcctgc gcgcggccca catggccccc
     5641 tccttcctgc cctggcaccg ccgcttcctg ctggagctgg agcgggcgct gcgccgggtg
     5701 gactcctccg tgaccgtgcc gtactgggac tggacccgag accgcggcac gacctcggct
     5761 ccctggacca aggacctcct cggcggcaac ggccgcaaga gcgaccggca ggtgaccacc
     5821 gggccgttcg cgtacgcggc cgggcactgg accatcaagg agggcatcac cgacggcaag
     5881 tacctcaccc gcgacctcgg ccgcgccgcc gccccgatcc agctcccgac caggagcgag
     5941 ctgcagtggg cgctggacga cccggcgtac gacgtctcgc cgtgggactc gaccgtcacg
     6001 cggggcttcc gcaacaagct ggaggggtgg gggacagggg gcggcagcgc ctcctggcac
     6061 aaccacaacc gggtgcaccg gtgggtcggg ggcgtgatgc tcggcggggc gtcggtcaac
     6121 gaccccgtgt tctggctgca ccacgccttc ctggactacc agtggtaccg ctggcagcag
     6181 gcgcacaaga accaccgcta cctgccggcg gcccagccgg gccccggtga caagcagcgc
     6241 ggccgggtcg tcgcgcggct cgacaagatg ccgccgtgga acgtgacacc ggactcgctg
     6301 gaggatgtga gcgggatcta ccggtacgcg tgaggacgtg tcgctcggac acgtcttcgg
     6361 ggcagggcac gcgaagagcc ccggcgctca gggtgccggg gctcttcgcg gagggtgcgg
     6421 ggctcagttg ccgtagccgt agtcgccctc ggggacctcc tgcacgggct ccggctcgcc
     6481 gacgttggcg caggtgttgc cgaaggcggg gttcagcagg ccgaccacgt tgatggtgtt
     6541 gccgcacagg ttgaccggga tgttgaccgg cacctggacc acgttgccgg agcccacgcc
     6601 gggcgagccg acggccgcgg ccccggcgtc cgcgtcggcc agcgccagac cggcgccacc
     6661 gaccatcacg gcaccggtgc cgagagccac gacggctgcc ttcgcaatgc gagacatcac
     6721 gttctcctta tgtcgcttca tgtcgctcgg gggagtggag cggccgaatt cgccgccgca
     6781 cttcccgtac aacggcgcgc ggaggagggg gtcacggggg aatggtgggg gatcaccctt
     6841 ttactggggg tgaatctcgg gtggggggat tcggggggat gtgtccggtg tgggaggcgt
     6901 ccggtcagcc ggtgaaagac tccggcccga accgccccca ggcgatgaag gccgcgagcg
     6961 cgaggtaggc cagattcagc gccacgaacc cgaactcgcg gcgccggccg tgggtggcca
     7021 tcgccccgat catcaacagc acccagcagg cggcggtcac gggaacgagg acgggcacac
     7081 gatcgaggag tgcgggcagt acgagtccga cggcggcgag cagttcgagc accccgagcg
     7141 ccttgacact ccgcgcgccg accccctcgg tccaccctcc cccgtgcacg gcggccagct
     7201 tctccttcgg cacgaaggtc ttgctgacac ccccgacgag agccacgacg gccagcgccc
     7261 aggcgctgat ccagagagcg aggtccatgt gtgccacttc cttaccggtc ggtgttcctt
     7321 ccggaaggga gacggggcgg cgcgccgctt tgtgacatgc accggctcag cgtggccgac
     7381 ctggcgccgc ccgccggccg agcccaccgc ctcggcgcct tcggcaggcg atctcgccgg
     7441 gtcggtggtc agaccgtggc gcacccttca cggtcggtgc gggtgagtat cgaacccagc
     7501 ggaaccatgt cgttggcctg cctgacggta caggggagtg gaacttcctt gagcctgccc
     7561 tcgtgcagca gtaacgccag tagttcggcc tggtcctcgg tgatcttctc cgactgagca
     7621 ctgaagttgg ccccgtgagc gtggtagctg gccgtctgag cggtccgggt ggcccacaag
     7681 gtggcgagcg agacgtgttc cgtgagagcg gcaggggctc gtgatccacc gcacattgtt
     7741 cggagcgatg actttgccgg ttcccgccgg tcgaatgagc gacaacatca cgcgaggaag
     7801 gcttactcgt gactcatacg ttcagcgcgt ccaagcacgt cgcccccgca tcgtccgtgg
     7861 acggcgcggc ggtacgccgg aggctggcgg gtggcatggc ggcaggtccc gtgttcctcg
     7921 cggtcggtgc cgtgcagggg ctcgcccgtg acggcttcga cttcagccga aacgcgctca
     7981 gccagctcgc gctcgggcag gcgggctggc tccagacggt gaactttctg ctcaccggcg
     8041 ccatgttggt catgggtgcg gtcggcctgc gccaggtgct gggcgggggt acaggcggca
     8101 cctgggcgcc gcttctggtc agtgtcttcg gcgtctcctt ctgggccgcg gcggccttcc
     8161 cggccgacgc cggcgcgggg ttccctgtcg gagccaccga cgggaccgtt gtcagcgagc
     8221 acggtgtcgt ccacatggtc gccggaatgg tcggctacct cgctctgtgc gccgccttcg
     8281 tcgtcctggc cggctcgctc gccgcccgag gccaccgcag gtgggccgtg acctcgcgcc
     8341 tcgttccggt cgcggtcctg gccggattcg tggcctccgc cgcctccgtc ctggccttca
     8401 ccgccggcgc gggcctgggc ctgctgtggc tgaccgcggt gacggcccgt ctggccaccg
     8461 ctccggtcga cgcctgaccg caagacgttt accgacacca tcaagctcct ccccacagaa
     8521 acgaggacac tccatggcca cgaagggccc tgccagctac ttcccctcca tcgagaagaa
     8581 gtacggcctc ccgatcaccg agtggaaggc gctcatccgc acctctccgc tcaccaagca
     8641 catggagctc gtcaactggc tcaagtccga gcacggcttg ggccacggcc acgccaacgc
     8701 cctcgtcgcc cacaccctca ccgaggacgc cggcaagtag tcagccacga ggccgcatcg
     8761 ggcttcctcg cccaggaggc ccggcaggcc gaagagtgga aagaggcgct cggtgtcccg
     8821 gaaggccgta acggcactga tccggtcgcc ggagaactcc acgacctgga gggcccacgg
     8881 cacgtagccg gtgccatccg ggtgggggcg gtactggccg aaggcggggg agccgttcgc
     8941 tgcggtaggc accagacgcg agccgcggca gccgatcgcg gggccggtga gccacgcctg
     9001 gatgctctgg agttcctcaa ccgacgccca cggacgatgc ttgcggttgt ccgacacgcc
     9061 ccttccgggt gacagtcgga ccgctcggac gggccggaga gggaaacatg atcaggtggg
     9121 tctggttcgg ccgggtgttc gcggtgacgg gcgccgtgct gggcggggtg ctggtctcgg
     9181 gcgtgcccgt gacgtccggc ccgccttcgg cggacgtgcg ggcgctgtcc gcgggcagcg
     9241 gccacgtgct ccgggaggag ggcagaggac acgacgagtg gctgacgatc gaccgtttgg
     9301 aacgggacgg cggggacctc ccgcgcgaag ggacgacgtt cgaggtgcgt gccgggttcg
     9361 agcacaaggg cgaacgggtg atgcggaaga gggcgttctc catctcggtg tccgagcggc
     9421 tctcgttcgc gacggagtac agcaactgcg agtacggcga ggacgtgccg acttccccgg
     9481 tgaaccggtc gttccgtaag gcggtgtgct ggatcgacgc gcggatcgag ccgggcgagt
     9541 ccgtcgacct cgcgcccatc cccctgaagg tggaccgttg ggcgaacgac gaaacggtgt
     9601 ccgtggcgga tgcgggagtg agagagcagt gggcggcgtc gggtgtcgtg tggcggaacc
     9661 ggcactgggg ccggggcggg aagctgaccc tcgcgaagcg ggccggagac gtgcctcctg
     9721 ccgcccgggt gaaagccgct gtcgatatgt cgtccggggg gttggacgtg aacgtcgaca
     9781 gctcgtggga catcgaggtg acgggcgccg cgctcaacgg gaggaggggc gaggcggaca
     9841 cggcggacct ggccatggac ttccacggtg ccgacgtcca ggcccagcgc gacccagaga
     9901 acagcgttcc gttcgcccgg gtccgcgtgc agttcccccg gggcgtgacc gtgctgagga
     9961 gtccgaagca gtgcgggaac agggtgacga agaggccgta ctaccaatgt gagtacaacc
    10021 tgtacaccaa cagctatgac gcgccgatgc tccgggacgg gtaccacgag gccttcccct
    10081 tcaggctgcg gatcgacgac ccgtccgagc tcaccggtgg ctcgatcagt ctcgacgccc
    10141 cgccggaggt gctgcaggac gacgccgacc acgcgaacag caccgcacgc atcaccgtag
    10201 aggccgtcgg cggaccggac cgaacggcct ggatcgtcgc cgcgggcgcc ggaacactgg
    10261 cgctgaccgg aatcctcgcc gccgtcctcc tccgcgcccg tcgacgcgcg gactgacggg
    10321 agcccggacg gacccctgag cggaggcggc ctggtacggc cccttacggc agggctcgaa
    10381 cctccggcca agcgcttgga aggcgtcggc ggctcggccg ctgtacccca tctgagctgc
    10441 acctatctgc gcactccgcc ccgtgaagcg tggccttcga cac
//
