LOCUS       NZ_SHKU01000001        10216 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Streptomyces sp. BK022 Ga0307664_101, whole genome shotgun sequence.
ACCESSION   NZ_SHKU01000001
VERSION     NZ_SHKU01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. BK022
  ORGANISM  Streptomyces sp. BK022
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1305487)
  AUTHORS   Kalluri,U.
  TITLE     Understanding the reciprocal impacts of modified plant cell wall and
            associated microbiome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1305487)
  AUTHORS   Kalluri,U., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 215.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004217355.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 02:58:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,482
            CDSs (total)                      :: 6,401
            Genes (coding)                    :: 6,280
            CDSs (with protein)               :: 6,280
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 49 of 121
            Pseudo Genes (incomplete)         :: 92 of 121
            Pseudo Genes (internal stop)      :: 16 of 121
            Pseudo Genes (multiple problems)  :: 29 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:05:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 908611
            Orig. end                         :: 918827
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SHKU01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1855..4089)
                     /locus_tag="EV284_RS04205"
                     /old_locus_tag="EV284_0850"
     CDS             complement(1855..4089)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115096.1"
                     /locus_tag="EV284_RS04205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_0850"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_207228901.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.6e-39, bitscore:
                     130.2, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPTDTAQENRGRRRAAPARRPVPKPRGGTVRTPTVLQMEAVEC
                     GAASLAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGLTAKGMQMDLAALA
                     EVRAPAILFWEFNHYVVYDGMGRRFGRRGVYINDPAKGRRFVPMEEFDGSFTGVVLVLE
                     PGDGFSRGGRKPGVLGAMPARLRGTAGTLPAAVLASLLLVAVGAAVPALSRTYIDMFLI
                     GGQTSLLGVLFTSMATCVALTLVLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFF
                     SQRSPADLVQRLQSNDQVAETLARDLAAAGVDAIVVVLYAVLLYTYDPQLTFVGIGVAL
                     LNIVAMRLVIRLRATRTAKLRADTARLTNTSYTGLQLIETMKATGGEDGYFRKWAGQHA
                     ATLEEQQKLGVPSAWLGVVAPTLATFNSALILWIGGLRAVEGHISVGLLVAFQALVARF
                     TAPLTRLNGVAGRIQDFAADVARLKDVENFQADPLYARPGGADSTRRLRGHVELEGVTF
                     GYSPLDKPLLTDFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYSPWEGVIRIDGRRLE
                     DIPRGALAASVSFVDQDVFLFEGSVRDNVALWDPSIPDEAVEEALRDAALYDVVARRPG
                     GVHSKVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELTVMDNLRRRG
                     CACVVIAHRLSTVRDSDEIVVLQHGTVVERGRHEELVARGGAYAALVRER"
     gene            complement(4086..4892)
                     /locus_tag="EV284_RS04210"
                     /old_locus_tag="EV284_0851"
     CDS             complement(4086..4892)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="EV284_RS04210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_0851"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_130330324.1"
                     /transl_table=11
                     /translation="MQFRQQALAKLQSPEDLDLPVRLARPQGWLVLGITVVVMAAASVW
                     AVTGSVASTVSAPAILTHGQGSYVLQSPVAGQVTGVLTRQGQRLPAGAPVLKVRTAQGD
                     SVIRTVAAGRITALAATIGQIVQTGADVAAVEKVAHDSDPLYATVYVPAENAASIPAHA
                     TVDLTVSSVPAERYGVLRGTVKSVDRTAQTPQSIGAFLGDSQLGKQFTSKGRPVAVLVS
                     LDKSASAKSGYRWSSAGGPPFGLDSMTLASGAVHLSDQRPIDWLLP"
     gene            complement(5001..5216)
                     /locus_tag="EV284_RS04215"
                     /old_locus_tag="EV284_0852"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="EV284_RS04215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_0852"
                     /product="hypothetical protein"
                     /protein_id="WP_031179346.1"
                     /sec_met_domain="strepbact (E-value: 4.5e-36, bitscore:
                     119.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPRVETVEISDAELDNVSGGLSVNALSTVTGAVNGIAPVSGL
                     VDTVVGTVEGVTGLNTAPVTGLVAGL"
     gene            5871..7250
                     /locus_tag="EV284_RS04220"
                     /old_locus_tag="EV284_0853"
     CDS             5871..7250
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548647.1"
                     /locus_tag="EV284_RS04220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_0853"
                     /product="S1 family peptidase"
                     /protein_id="WP_130330326.1"
                     /transl_table=11
                     /translation="MPKRKAAIAVGGVAALGAAALLLPNANASQDKDTAATGTARTLKA
                     TDATTLAAQLKQSLGGQFAGSYYDSAKRQLVVNAVGDGAAVARAKSAGAVVRQVENSTA
                     DLQSAARTLRTKAAIPGTAWAVDPRTNKVIVSADSTVTGAKWDRLESTVDGLGSGTATL
                     KKTGGTFKTFVSGGDAIFSQVQGGGVRCSLGFNVTASDGSPAFLTAGHCGVAAKEWSDS
                     QTGQPIATVDQATFPGEGDFSLVKYDDPATQAPSEVNAGNGQVVAITQAAEATVGSTVF
                     RMGSTTGLHDGQVTGLDATVNFQSETDPNGVDTVTGLIQTDVCAEPGDSGGSLFTQEGA
                     AIGLTSGGSGDCTSGGETFFQPVTAALAATGATLGDGGAGAGAGDGQAGAGDQAGAGDQ
                     SGAGDQAGAGDQSGAGAGDQAGGDQIGGADDQAGAGAGAAQEQGTKHHHRNRQQGGTAS
                     N"
     gene            complement(7305..9428)
                     /locus_tag="EV284_RS04225"
                     /old_locus_tag="EV284_0854"
     CDS             complement(7305..9428)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117170.1"
                     /locus_tag="EV284_RS04225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV284_0854"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_130330328.1"
                     /transl_table=11
                     /translation="MSDTPDPARETAEAGRPPLSVALTAMMEDVGAHSGAVYLLRPDVP
                     VLEMAVMAGLPRAFAAPWERVGVSAPIPVAQAVRQRRPVWVGGGEEMARRFPRIAMVLP
                     YPFALAALPLATDDAVLGSVFVTWPAGHGPELTERERTLLAAGCERLTRVLARASAGNL
                     SLGSERDLVATPDAAVVDTLGPVEAARMVARLPYGLVSLDLHGRIGHVNAAAAELLGRP
                     AAELLGTLPWVSVPWLNDPLYEDRYRAALISRRPASFVALRPPGHWLSFRLYPGSTGVS
                     VRITRVRVLTERTRVPAGARPHRLVTISRLLSLTGALTEAVAVEDLVQLVADEVGPAVG
                     SHALMLLAAEEGRMRVLGHRGYRDERVAGWFDGMPLSERTPGTQALSSGVPAFFESLEQ
                     LERLYPVRRESRDGFSAWAFLPLVASGRPVGTCVLAYAEPRSFPAEERAVLTSLGGLIA
                     QALERTMLYDAKHRLAHGLQEALLPHSLSPPPGIEAAARYLPATRGMDIGGDFYDLVPT
                     RPRASAVIGDVQGHNVTAAALMGQIRTGVRAYSTVGQAPGEVMRSINRLLIDLGTELFA
                     SCLYLRLDPAHGRAVMARAGHPPPLLRRPDGRVRVLDLAGGPLLGIDSEAAYPTTEVPL
                     TPGSVLALYTDGLVESPGVDIEDALTDLGALFSEAGDGPLEELADALVRHGTAGRDRVD
                     DVAVLLLRAQDGM"
     misc_feature    complement(9840..9854)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 gcgcccagca ccgagctgag gttcttgatc cggccgacct tctgctggag ctcccggctg
       61 cgcgcgaact cccgggccca ggcggcgtag gcgtagttct ccgccgacgc cacccgcagc
      121 ttgggcagcc cgcgcagggt ctggaacgcc tggttgttca gtttgttgcc gagcgtcaca
      181 agacgccgct gccagcgcac ctgccacagt ccgagccccg tgaagacggc gccgatcacc
      241 aggagcatcg cgatcgcggc catcgccatc ggcacgttgt agtagagcag caggccgacg
      301 ttcatcgcgc cgatcgtcac cgactgcgcc accaccgggc cgacgcccgc cagcagccgc
      361 cggatcgagc tgatgcccat cgcggcactc gccagctcgc cggtggagcg cccgctgaag
      421 aaccgggtcg gcagccgcag cagccggtcc cagaccgccg gctggagcga gccctctatg
      481 cgcccctcca ggcgcaggat ggtgaggttc tccagcagcg tgaacgccgc cgacaccacc
      541 ccgctgagca tcaccgcgag acacacctgc gcgatcaggt tctcccgcgc ctgcggcacg
      601 tactcgccga gcaccttgcc cgtggcgacg ggcaccagcg cgccgatcgc caccgtgacc
      661 aaccccgcca gcagcaggtt cagcacgtcg ttccgcgtgc cgcccaggct gaaccggagc
      721 aggcccagcg ggccgagccg cttctcgggc agcggccggt agaacatcac cgcccgcggc
      781 tcgaactcct ccgcgttcgc cttctccacc ggcgtctccc ggccggtcgc cggctgtacg
      841 gccacatagc cgccccggcg ccacagcagc gccaccggcg cacccgagag cgcccggtga
      901 ccgaccagcg ggcccacgtt ctcccgccac cagcggccct ccaggcgtac cgcccggacc
      961 cttacgcgcg aggccagcgc cacccgctcc accgggtcca gccgctcgct ctcgccgatc
     1021 ccgcgcgccg ggtcggccag cgtgatcccg gccgcctgcg cgaccagctt gcaggccgcg
     1081 taaccggcgt ccgcgtcggc cgccgtcgtc cgccggtccg agcgcttgcc gatggaggcg
     1141 agcaaggtct ggtcggcctg ggcgcgtacc gcctcaccgg ccttgatgcc ggccgccgcc
     1201 cgcgtctcgt gggtgcgctc cagctgctcg atccagcggt ccagcgcggt cagcagccgg
     1261 tactgctggt cgaccaggct ctgccacagc cccgggtcca tcagcaggtc ggccgccgcc
     1321 tccgcgccgt acaccgagcc gtactgcacg ctgcccggcg gcacctgcat ccagaacacg
     1381 tcgtcgtccg agggcgccac cgacttgtcc tcggcgagcg gcgcctggaa cagcaccgag
     1441 agaccccggc cgacgccgag cgcgagggcg tactccagcg ggctggagga gggcggcacg
     1501 tactgcgggt tgccgtactc gtcgtaggac caggtctcgg tctgcgcgcc ctggtacagc
     1561 tcgcgcaggt cgacccggtg caccacgcag tcccgcaccg ggcgggcgac cagggagtgc
     1621 tggggtccgg cgaccgggcc gagcagcagc gcgcccgcct ccagccggcc caggtggtgc
     1681 cagtgaccct gctcggcggc gtccaccgcg aacaggtcga gcgcgccggc ggccaccagc
     1741 cagaggacct gtggtccttc gagatcgagc cggttcaggc cggcgcagtc gacccgcgta
     1801 cccagcgagc cgagggcgcc cagcaccaga tcaccgtcgt gcgtggacgt catctcaccg
     1861 ctccctgacc agcgccgcgt acgcgccacc gcgcgccacc agctcctcgt gcctgccccg
     1921 ctccacgacc gtgccgtgct ggagcaccac gatctcgtcg ctgtcgcgga ccgtggacag
     1981 ccggtgggcg atcaccacac aggcgcagcc gcgccggcgc aggttgtcca tcaccgtcag
     2041 ctccgtctcc gcgtccagcg cgctggtcac ctcgtccagc accaggatgc tggggcggcg
     2101 caccagcgcc cgcgcgatct ccagccgctg gcgctgcccg ccggagaagt tgcggccgtc
     2161 ctgctcgacc ttgctgtgca ccccgcccgg cctgcgggcc accacgtcgt acagcgccgc
     2221 gtcccgcagc gcctcctcca ccgcctcgtc cgggatcgag gggtcccaca gcgccacgtt
     2281 gtcgcggacc gagccctcga agaggaacac gtcctggtcg acgaaggaca ccgaggcggc
     2341 gagcgcgccg cgcgggatgt cctccagcct gcggccgtcg atccggatca cgccctccca
     2401 gggcgagtac aggccggaga tcagccggga caccgtcgac ttgccgctgc cggagccgcc
     2461 caccagcgcc acctgctgac ccggcccgac cgtgaggtcg aagtccgtca gcagcggctt
     2521 gtccagcggg ctgtagccga aggtgacgcc ctccagctcc acgtgcccgc gcagcctgcg
     2581 ggtggagtcg gcgccgccgg gacgcgcgta cagcgggtcg gcctggaagt tctccacgtc
     2641 cttcagccgg gccacgtcgg ccgcgaagtc ctggatgcgg cccgccacgc cgttgagccg
     2701 ggtgagcggc gcggtgaagc gggccaccag cgcctggaac gccaccagca gaccgaccga
     2761 gatgtgcccc tcgaccgccc ggagaccgcc gatccacagg atcagcgcgc tgttgaaggt
     2821 ggccagcgtc ggcgcgacca cgcccagcca ggcgctcggc accccgagct tctgctgctc
     2881 ctccagcgtc gccgcgtgct gccccgccca cttgcggaag tagccgtcct ccccgccggt
     2941 cgccttcatc gtctcgatga gctggagccc ggtgtacgag gtgttcgtca gccgcgcggt
     3001 gtccgcccgc agcttcgcgg tacgggtcgc gcgcagccgg atcaccagcc gcatcgccac
     3061 gatgttcagc agcgccacgc cgatgccgac gaaggtgagc tgcgggtcgt aggtgtacag
     3121 caggaccgcg tacagcacga ccacgatcgc gtccacgccc gccgccgcga ggtcgcgggc
     3181 cagtgtctcg gcgacctggt cgttcgactg caggcgctgc accaggtcgg cgggggagcg
     3241 ctgggagaag aaggtgaccg gcagccgcag caggtggcgc aggaaccgcg cgctggacag
     3301 cgtggaggag atgatccggc cgtgcagcag gttggcctgc tggagccagg tcagcaccag
     3361 ggtcagcgcc acacaggtcg ccatcgacgt gaacagcacg ccgagcagcg aggtctggcc
     3421 gccgatcagg aacatgtcga tgtaggtacg gctcagcgcg gggaccgccg cgcccaccgc
     3481 gaccagcagc agactcgcca gcaccgccgc gggcagggtg cccgccgtgc cgcgcaaccg
     3541 ggcgggcatc gcgccgagca caccgggctt gcggccgccc ctgctgaagc cgtcgcccgg
     3601 ctccagcacc agcaccacac cggtgaagct gccgtcgaac tcctccatgg gcacgaaccg
     3661 gcggcccttg gcggggtcgt tgatgtacac cccgcgccgg ccgaagcggc ggcccatgcc
     3721 gtcgtagacg acgtagtggt tgaactccca gaacaggatg gccggggcgc gcacctcggc
     3781 gagcgccgcg aggtccatct gcatgccctt ggcggtcagc ccgtaactcc gggccgcctt
     3841 cagcaggttg ctcgcgcgcg agccgtcgcg ggagacaccg caggcgatgc ggagctcctc
     3901 cagcgggaca tggcggccgt agtggccgag caccatggcg agggaggcgg cgccgcactc
     3961 gacggcctcc atctggagca cggtgggcgt acggaccgtg ccgccacggg gtttgggcac
     4021 cgggcggcgg gccggcgcgg cccgtctgcg gccccggttc tcctgcgcgg tgtcggtcgg
     4081 ggcggtcacg ggagcagcca atcgatcggg cgctggtcgc tcagatggac cgcaccggag
     4141 gccagggtca tggagtccag cccgaacggc ggcccgcccg ccgacgacca gcggtaaccg
     4201 ctcttcgcgg aggccgactt gtccagcgag acgaggacgg cgaccgggcg gcccttgctg
     4261 gtgaactgct tgccgagctg gctgtcgccg aggaacgccc cgatcgactg cggggtctgc
     4321 gccgtgcggt ccaccgactt caccgtgccg cgcagcacgc cgtagcgctc ggccggtacg
     4381 gaggacacgg tcaggtccac ggtggcgtgc gcggggatcg aggccgcgtt ctcggcgggg
     4441 acgtacaccg tcgcgtacag ggggtcggag tcgtgtgcga ccttctccac ggcggccacg
     4501 tcggcgccgg tctgcacgat ctgcccgatg gtcgcggcca gcgcggtgat ccggcccgcg
     4561 gcgacggtac ggatcaccga atcgccttgc gcggtccgga ccttgagcac cggggcgccg
     4621 gcgggcaggc gttgcccctg cctggtgagc acaccggtga cctggccggc cacggggctc
     4681 tggaggacgt aactgccctg cccgtgcgtg aggatggccg gggcgctcac ggtggaggcg
     4741 accgaaccgg tcaccgccca cactgatgcg gccgccatga cgacgacggt gataccgagg
     4801 acgagccacc cctgcggacg tgccagccgc accgggaggt cgagatcctc cggcgactgg
     4861 agcttggcga gggcctgttg gcggaactgc acggaacttc cctcacccga ggagagacga
     4921 cggcacggaa aggtttcggg acgcccgaga gtcccggacc cgcgaggggg tccgggactc
     4981 tcggtgacac cgaggtgcgg tcagagaccg gcgaccaggc cggtgaccgg ggcggtgttg
     5041 aggccggtga caccctcgac ggtgccgacg acggtgtcca ccaggccgga aaccggggcg
     5101 atgccgttga cggcgccggt cacggtggag agggcgttca cggacagacc gccggaaacg
     5161 ttgtccagct cggcgtccga gatctcgacg gtctcgacac ggggggtgga gttcatggga
     5221 gaacttccct tcgtataggt cttcataagg ggggagcggc cccctctggg gacagatgac
     5281 ggccgcgacc gcgaggagtt ccagaccttc cggccgccgt ctccggcagc catggggcgg
     5341 ttcctgcgat gcgatggatc aaagcacgca cgccgcgccc ggttccactt actcagggct
     5401 ctcaccagcg agttcggtgc agggtggggg tcattcgtgc agggaggcgc actccctggg
     5461 cggttttcct ccacattccc cgcgccgtcg tcgcaccgtc tccactaccg ccggtaacgc
     5521 gaatccggca cacctctcca aaggcatgtc cggggcaccc gtaccggcgc gcggacccct
     5581 cggtccgtgc cccgcctggg tgcgggatgt gcagattccc tgaagccggg attccgttac
     5641 cggtgttgga cgcagggcga cccggtcgtg acaaggggtg accgaggtgc cggtgtgtca
     5701 gaagaggcgt gggcgtggct gattccccac ccgcacaacg aagttggcaa tcgggattga
     5761 acgcgccggg cggaccgccc gtacctgtag ccagccggca cccccctacg aactgcccga
     5821 cagcggcaga cccccgcacc caggaggtcg agaagttgag tcacaagcgc atgcccaaga
     5881 ggaaggccgc gatagcggtc ggcggtgtcg cggcgctcgg agcggcggcc ctgctgctgc
     5941 ccaacgccaa cgcgtcccag gacaaggaca cggccgcgac gggcaccgcg aggaccctca
     6001 aggccaccga cgcgaccacc ctcgccgccc agttgaagca gtccctgggc ggccagttcg
     6061 ccggctccta ctacgacagc gccaagcggc agctcgtcgt caacgccgtc ggtgacggtg
     6121 ccgccgtcgc acgtgcgaag agtgcgggcg ccgtcgtacg gcaggtggag aacagcaccg
     6181 ccgacctcca gtccgccgcg cggaccctgc ggaccaaggc ggccatcccc ggtaccgcct
     6241 gggccgtcga cccgcgcacc aacaaggtga tcgtcagcgc cgactccacg gtgaccggcg
     6301 ccaagtggga ccggctggag tcgaccgtcg acggtctcgg gtccggcacg gccacgctga
     6361 agaagaccgg cggcaccttc aagaccttcg tctccggcgg cgacgcgatc ttctcccagg
     6421 tgcagggcgg cggcgtccgc tgctccctcg gcttcaacgt caccgcctcc gacggcagcc
     6481 ccgccttcct gaccgccggt cactgcgggg tcgcggccaa ggagtggtcc gactcgcaga
     6541 cggggcagcc catcgccacc gtcgaccagg ccaccttccc cggcgagggc gacttctccc
     6601 tcgtcaagta cgacgacccg gcgacccagg cgcccagcga ggtcaacgcg ggcaacggcc
     6661 aggtcgtggc gatcacccag gccgccgagg ccaccgtcgg ctccaccgtg ttccggatgg
     6721 gctccaccac cgggctgcac gacggccagg tcaccggcct ggacgccacc gtgaacttcc
     6781 agagcgagac cgacccgaac ggtgtcgaca ccgtcaccgg actcatccag accgacgtgt
     6841 gcgccgagcc cggcgacagc ggcggctcgc tgttcaccca ggagggcgcg gcgatcggcc
     6901 tgacctccgg cggcagcggc gactgcacca gcggcggcga gaccttcttc cagccggtca
     6961 ccgccgccct cgcggccacg ggcgccaccc tcggcgacgg gggcgcgggt gccggtgccg
     7021 gtgacggcca ggcgggtgct ggcgaccagg cgggcgccgg ggaccagtcc ggcgcgggcg
     7081 accaggcggg tgccggggac cagtccggtg ccggtgccgg tgaccaggcg ggcggtgacc
     7141 agatcggcgg cgccgacgac caggccggcg cgggtgcggg agccgctcag gagcagggca
     7201 ccaagcacca ccaccgcaac cgtcagcagg gcggaaccgc ctccaactga ccgaccgggt
     7261 ccggcccttc ggcggagggg ccggaccctt tgcgcggcgg acgctcacat accgtcctgc
     7321 gcccggagca gcagcaccgc cacgtcgtcc acccggtccc gccccgccgt gccgtgccgg
     7381 accagcgcgt ccgccagttc ctccagcgga ccgtcgcccg cctcggagaa cagcgcgccc
     7441 aggtcggtca gcgcgtcctc gatgtccaca ccgggcgact ccaccagtcc gtccgtataa
     7501 agggcgagca ccgagcccgg ggtgagcggc acctccgtcg tcgggtaggc cgcctccgag
     7561 tcgatgccga gcagcgggcc cccggccagg tccagcaccc gtacccgccc gtccggacgt
     7621 ctgagcagcg gcggcggatg accggcccgc gccatcacgg cacggccgtg cgcggggtcg
     7681 aggcgcagat agaggcaact ggcgaacagc tcggtaccga ggtcgatcag cagccggttg
     7741 atgctgcgca tcacctcgcc cggtgcctgc cccaccgtgc tgtacgcgcg gacaccggtg
     7801 cggatctggc ccatcagcgc cgccgccgtc acgttgtgcc cctgcacgtc gccgatcacc
     7861 gcggacgccc gcggccgggt cggcaccagg tcgtagaaat cgccgccgat gtccatgccc
     7921 cgtgtcgccg ggagatagcg cgcggcggcc tcgatgccgg gcggcgggga cagcgagtgc
     7981 ggcagcagcg cctcctggag cccgtgcgcc agccggtgct tggcgtcgta cagcatcgtc
     8041 cgctccagcg cctgggcgat cagcccgccc aggctggtca gcaccgcccg ctcctcggcg
     8101 gggaaggaac gcggctcggc gtacgccagc acacacgtcc ccaccgggcg cccggacgcg
     8161 accaacggca gaaaggccca cgccgagaag ccgtcacggg actcgcgtcg cacgggatac
     8221 agccgctcca actgctccag agactcgaag aacgccggca ctccactgct cagcgcctgg
     8281 gtgcccgggg tgcgttcgct cagcggcatc ccgtcgaacc agccggccac ccgctcgtcc
     8341 cggtagccgc ggtgcccgag cacccgcatc cgcccctcct cggcggcgag cagcatcagc
     8401 gcgtgactgc cgaccgccgg acccacctcg tccgcgacca gctggacgag atcctcgacc
     8461 gccaccgcct ccgtcagcgc ccccgtcagg ctcagcagcc gcgagatcgt caccagccgg
     8521 tgcggccgcg ccccggccgg cacacgcgtg cgctcggtca gcacccggac ccgcgtgatg
     8581 cgcacgctca ccccggtgct gcccggatag agccggaagg acagccagtg gccgggtggc
     8641 cgcagggcca cgaaggaggc cggacggcgg ctgatcagcg cggcccggta acggtcctcg
     8701 tacagcggat cgttcagcca cggcaccgac acccagggca gcgtgccgag cagttcggcg
     8761 gcgggtcggc cgaggagctc cgcggcggcc gcgttgacgt ggccgatccg gccgtgcagg
     8821 tcgagcgaca ccagcccgta cggcagccgg gccaccatcc gcgccgcctc aaccggtccc
     8881 agcgtgtcca cgacggccgc gtccggtgtc gccaccagat cccgttcgga gcccagcgac
     8941 agattcccgg ccgacgcccg cgccagcacc cgggtcagcc gctcgcaccc cgccgccagc
     9001 agcgtccgct cccgctcggt cagctccggg ccgtgcccgg ccggccaggt cacgaacacc
     9061 gagccgagca cggcgtcgtc cgtggccagc ggcagcgccg ccagcgcgaa ggggtacggc
     9121 agcaccatcg cgatgcgcgg gaagcggcgt gccatctcct cgccgccgcc gacccacacc
     9181 ggccgccgct gccgcaccgc ctgcgcgacc gggatgggcg cgctcacccc gacccgctcc
     9241 cacggcgcgg cgaacgccct gggcaggccc gccatcaccg ccatctccag cacgggcaca
     9301 tcgggccgca gcaggtagac cgcgcccgag tgcgcgccca cgtcctccat catcgcggtc
     9361 agggcgaccg acagcggcgg ccgtcccgcc tccgccgtct cccgcgcggg gtccggcgtg
     9421 tcggacacgc cgaccacctc ctcgtacggc cctcgcgccc cggactgcgg acgagtccca
     9481 gctctgggag acacgccgag taccccctcc cgacgccgct catgccgagc ggcggaaaag
     9541 gccggtacac gcatgcatga ggcttacggg ccaatcgcgc ggtagggaaa aaccgcgcgt
     9601 ggtcggtgca caggggctgc gccacgcgcc cgcacgcgct gtaatggcca acgtggtggg
     9661 tgagggtgct gcggtgcgca ggacgggagc cgggcgtgcc gagcacgtcc tcgccacact
     9721 catggccgcg cccccctcct ccgaccggct ccgccgcgtc ctggaacagg ccctcgtcct
     9781 cgccggcgcc tcgttcgccg gcgtgtacgc ccccggtgac gaccccgcag tgctgtgcct
     9841 ggccgagtcc gtcggactgc ccggctcgct gtacggcctg cgcgacagct accccgcggg
     9901 cggcggcgcc ccggtcgccg gggcccaccg caccggccgc cccctctggc tcggccccgg
     9961 cgaagtggcc gaccgggccg agggccgccg taccccggca cccgacttct ccctcgccgc
    10021 cctgcccctc gccgggcccg gcggcggctc gctgctggcg gtcgcggaac ggcccgaggg
    10081 gttcgacgcg ggggagaggg ccgcgctgga actcatcgcc cgcgccgtcg ccgtccccga
    10141 gcccggcccc gccgactccc gcgaactggc cccggacgcc ttcagcctcg ccatggacac
    10201 cgggcgggtg gaggtc
//
