LOCUS       NZ_SGWQ01000001        11827 bp    DNA     linear   CON 10-APR-2024
DEFINITION  Herbihabitans rhizosphaerae strain DSM 101727 Ga0310478_101, whole
            genome shotgun sequence.
ACCESSION   NZ_SGWQ01000001
VERSION     NZ_SGWQ01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Herbihabitans rhizosphaerae
  ORGANISM  Herbihabitans rhizosphaerae
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Herbihabitans.
REFERENCE   1  (bases 1 to 1096878)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1096878)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 226.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004216555.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 19:27:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,690
            CDSs (total)                      :: 6,628
            Genes (coding)                    :: 6,561
            CDSs (with protein)               :: 6,561
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 13 of 67
            Pseudo Genes (incomplete)         :: 59 of 67
            Pseudo Genes (internal stop)      :: 5 of 67
            Pseudo Genes (multiple problems)  :: 9 of 67
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:31:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1010417
            Orig. end                         :: 1022244
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SGWQ01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11827
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11827
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11827
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6827](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6827
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    295..310
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: medium, score: 26.61"
                     /tool="antismash"
     gene            complement(472..1686)
                     /gene="glf"
                     /locus_tag="EV193_RS05090"
                     /old_locus_tag="EV193_1011031"
     CDS             complement(472..1686)
                     /EC_number="5.4.99.9"
                     /GO_function="GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /gene="glf"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501292.1"
                     /locus_tag="EV193_RS05090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011031"
                     /product="UDP-galactopyranose mutase"
                     /protein_id="WP_130342723.1"
                     /transl_table=11
                     /translation="MGETDGGPLGQADLVVVGSGFFGLTIAERVATQLNKRVLVLERRP
                     HIGGNAYSEPDPDTGIEVHVYGAHLFHTSNKRVWDYVNQFTEFTNYQHRVFAKCQGQVY
                     SFPMNLGLINQFFGKSHTPDEARALIAEQASEIDTADAQNLEEKAISLIGRPLYEAFIR
                     GYNVKQWQTDPKELDASIITRLPVRYTFNNRYFNDTYEGLPKDGYTAWLEKMAEHPNIE
                     VRLNVDYFDVRDQIPAGTPTVYTGPLDRYFDYSEGRLGWRTLDFEREVVDTGDFQGTSV
                     MNYNDADVPYTRIHEFRHFHPEREDRHPKDKTVIVREYSRFATKDDDEPYYPINTADNR
                     AKLERYRELAKAEARERNLIFGGRLGTYKYLDMHMAIGSALSVFDNKVAPHLTEGAPLD
                     GSIDG"
     gene            complement(1735..2376)
                     /locus_tag="EV193_RS05095"
                     /old_locus_tag="EV193_1011032"
     CDS             complement(1735..2376)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808091.1"
                     /locus_tag="EV193_RS05095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011032"
                     /product="endonuclease"
                     /protein_id="WP_130342724.1"
                     /transl_table=11
                     /translation="MAQRDIARGLLAAAGRTYAAQAGIRLTDTPAPLYRLLVLSVLLST
                     RIKADIAVSAARELVRSGFGSPRRMLDATWQQRVDALGRAHYVRYDESTATALGDGARL
                     LIDTYRGDLRRLRDRAEGDQGKIRALLTEVPRLGPVGADIFCREAQQVWPELRPYLDKK
                     VLAGASRVGLPADERMLARLVDADDLAAFTASLVRVALDRDLAKSVTEGS"
     gene            complement(2405..3283)
                     /locus_tag="EV193_RS05100"
                     /old_locus_tag="EV193_1011033"
     CDS             complement(2405..3283)
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666839.1"
                     /locus_tag="EV193_RS05100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011033"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_130342725.1"
                     /transl_table=11
                     /translation="MRVGIAILPEYRWRQAAEKWRAAEEYGFHHAWTMDHLGWRSLVDG
                     PWVGAVPTLTAAATVTSRIRLGTFVASPNFRHPVPFARELMAVDDISDGRFTLGVGAGG
                     GGYDSTVMGGEDLAPVAKHARFTEFVTLLDALLTGERTTFRGEYFTAVEARNAPGCVQR
                     PRLPFVIAANGPKGMRLAAKLGAGWATTGGPWRGGDTDGMDQWWGSVAAVVRKFDETVA
                     AQGVQRFLQLDAAPAYVLSSMEFFADTLGRAAELGFTDVVAPWPRAEGIFAGDESVLER
                     VASDVLPTLEV"
     gene            complement(3312..4541)
                     /locus_tag="EV193_RS05105"
                     /old_locus_tag="EV193_1011034"
     CDS             complement(3312..4541)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330633.1"
                     /locus_tag="EV193_RS05105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011034"
                     /product="MFS transporter"
                     /protein_id="WP_242613141.1"
                     /transl_table=11
                     /translation="MNWLLSSSALSNLGDGIGQVAFPLVAAMLTRDPVLIAGLAVAQML
                     PWLLFGAVAGVLLDRVDRRRAMLVANVLHAVVVGALAIMVLAGTASIPIVYVAALLMGT
                     AETVADSAATVLIPALVDKSDLESANSKLQSTEIVGQIFVGNPVGSLAFAVFAAFPFLL
                     NTAAYVLAAILLVGLAGSYSARQAADVEPVRGMRAEFAVGVRWLRGSPLILRLIVLAGL
                     LGMTMEFAQAQLVLYALEDLRLSEAAFGVFAFAAGVGGLAGTGIAARLVARFSRWSVLL
                     AGIVVSGLAIGAMGLVHQPVLAAVLLGCFGGAVIVINVVVGTLRHLAVPDALLGRVIGV
                     WRTLVWGAVPVGALIGGLLTRWLHSPSWTFVVSGASQVVIAGAAAMFLRPFRARINGRP
                     ALSAGADTVA"
     gene            4786..4965
                     /locus_tag="EV193_RS05110"
                     /old_locus_tag="EV193_1011035"
     CDS             4786..4965
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EV193_RS05110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EV193_1011035"
                     /product="hypothetical protein"
                     /protein_id="WP_130342726.1"
                     /transl_table=11
                     /translation="MIENSDSGSMTIKHLAKDVRPGKKGTRVILGASDLLPGCAFEAEE
                     PNPAAGGRPPLAEH"
     gene            5001..6827
                     /locus_tag="EV193_RS05115"
                     /old_locus_tag="EV193_1011036"
     CDS             5001..6827
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="EV193_RS05115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011036"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_130342727.1"
                     /sec_met_domain="TIGR03975 (E-value: 1e-164, bitscore:
                     545.3, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTCLVCMPWHDLSRPSLAIGVLRTACRNKGIDAPIGYHGGLRFA
                     DFLAERTGGAFSPFEYTAIAEAGFNHALGDWIFAGTLFGADFGIEPMREYAERRNLRMR
                     SASAAREHADEFVDLVVDELAALDVELIGFTTTFMQNVPSLAVATRLKQRRPEIAVVFG
                     GGNCDGPMGIALHREFEAIDFVLRGECDESYPLLVQTLSGAPDRLDAVPGLCWRTPAGA
                     TVANPPGRLVPPAHLVAPDFDDWFAAFEKSTVSEHSAAELVVESARGCWWGEHHHCTFC
                     GLNGTAMAFRAKEPEVFTTEVEHLITRHAVLDVMVVDNILEPTYLRTALPEIAGRGWDL
                     RLHYEVKANLTRTQIDVLDAAGVRSVQPGIESLVDDVLARMNKGVRAVHNVRTMRDLES
                     AGLTVSWNWLYGFPGERAEDYAAVLPQLPALVHLQPPSCTARIELNRFSPYFADPSLGF
                     PVRTPAEVYRHVYDRPSERLHDLVYLFDTPDRGLTAEQVAPLNEAVRRWIDGYPDSSLV
                     STRTAAGIVIRDRRIGWPAADHLLDDERQCVAWQELEHGRSVRGLLRRLADAGLAWDED
                     GLTGWLDELRAHGLVFTEGGRWVGLATSPRGG"
     gene            6834..7403
                     /locus_tag="EV193_RS05120"
                     /old_locus_tag="EV193_1011037"
     CDS             6834..7403
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.3"
                     /locus_tag="EV193_RS05120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011037"
                     /product="DUF5825 family protein"
                     /protein_id="WP_130342728.1"
                     /transl_table=11
                     /translation="MRTAEPGELWPDHLVVPAAELTVARARGVVRRAQAFARRLVITDP
                     LSFGERAPAVLRLLTDATARRVPLEWTLGGEPPWPVRTLVHLTPPAGRGEYARRWRDGH
                     RAGLCCYRVGPGFVRVRDLRPDGEHRDVLITGALANRFVALAEDAAADEALLADLVDTG
                     LAVRVDEGHHVLPHRLLRWPIPHTEI"
     gene            complement(7497..8387)
                     /locus_tag="EV193_RS05125"
                     /old_locus_tag="EV193_1011038"
     CDS             complement(7497..8387)
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663389.1"
                     /locus_tag="EV193_RS05125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011038"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_130342729.1"
                     /transl_table=11
                     /translation="MRVGVVILPEYRWSEAAPKWRAAEEYGFHTAWTYDHLGWRSLVDG
                     PWFDSIGLLTAAATVTSSLRLGTLVASPNFRHPVSFARSLIALDDISDGRFTLGVGAGH
                     AGQDKYDNVVMGTPEPSMRERAERFAEFVELTDALLTTPKTSFEGKHFSAVQARMAPGC
                     AQKPRVPFMIAGTGPKAMRLAARFGQGWITTGTVRDDVDGWWGSLRAQVERFDEALHEQ
                     GRDSAGVARCLLLDASPVYSLSTVDTFAEMVGRAGEFGFTDVVVHWPRPDGIYAGDESV
                     LERVASDVLPTLTVQ"
     gene            complement(8403..9293)
                     /locus_tag="EV193_RS05130"
                     /old_locus_tag="EV193_1011039"
     CDS             complement(8403..9293)
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666839.1"
                     /locus_tag="EV193_RS05130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011039"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_130342730.1"
                     /transl_table=11
                     /translation="MRVGILIHPEHRWWIAEPKWRAVEEYGFHHAWTSDHLGWRTLVDG
                     PWFGAMPTLTAAATVTTKIRLGTWVASPQFRHPVSFAREIITLDDVCDGRFTLGVGSGG
                     SGDYDQRVMGGEPLSAKEKGVRFGEFVELLDQLLRHDRTSWSGEYFEAVEARNAPGCVQ
                     KPRVPFLIAANGPKGMRLAARHGTGWATIGGGEWQESDDMTAWWRGVAEVARKFDDAVE
                     ADGRTVPIDRYLGLDAAPQYVLSSLEAFTDALGRAEELGFTDVVAPWPRHDGIYAGSES
                     VLEQVASDVLPALSR"
     gene            complement(9391..10674)
                     /locus_tag="EV193_RS05135"
                     /old_locus_tag="EV193_1011040"
     CDS             complement(9391..10674)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EV193_RS05135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EV193_1011040"
                     /product="hypothetical protein"
                     /protein_id="WP_130342731.1"
                     /transl_table=11
                     /translation="MSIDDFDRPDRTIPDDVRNRLRDKVFRGIDGRSTRWYAPIAAAAA
                     VAVAAGAVAIAQPFGNGEDTAAPVLSPSTSKPTTTTSSPPNYAEPPLDPVKAKVQLDRC
                     WAAALRTGAAARLPDRARWLPVFTVDDFRGTTVTAAHADGKPLFCETTRTKVTLTDPNA
                     APAHLSRGRTSVALISPQGTLAGVLDPAWREPNFRAEGDGMSGGGPAWHKDRLFVFTSA
                     VTPRPGKVTAITADGELRVPTPWPPAVAVTEPIGPPPDRTSERGKALDACIAAAMSQNG
                     VPDPESWHPGAAAGSGDELVAFAVAGDRVASCTDQGAKRHPQFGVYQEKRATPDAPAKP
                     RVLALPPVIGGKIAVGGTLLPEARRMEITVPGRPPVSADVHDATFAALLLPKENTLERA
                     QMPDLNGIVCTVYDARGTVLYTGKLYGE"
     gene            complement(10671..11249)
                     /locus_tag="EV193_RS05140"
                     /old_locus_tag="EV193_1011041"
     CDS             complement(10671..11249)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673489.1"
                     /locus_tag="EV193_RS05140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_1011041"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_242613165.1"
                     /transl_table=11
                     /translation="MLTLPVPEAAEHTDAELWRRAADGGKADFGVLFQRHVQRLWNHAY
                     RLTGSWAQAEDLTSATFLTAWRRRAEITLVRDSALPWLYTVMGNLARDERRGLLRRLRL
                     VKKVSEPVAVADHADSVAARVDDEARARRAADAITALPPAQRRVVELCLVAGLAPAEAA
                     EVTGIDQGTVRGHLSRARARLRQTLEESE"
     misc_feature    11753..11766
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.79"
                     /tool="antismash"
ORIGIN
        1 tagtgcacgc cgagcaccag cctcgacacc agcatcggcg gaaccagcac cgggaccagc
       61 ttgcgcccgg tcatggtgga gaacagcacc gccgccgccg tggtcgaggt cgcgtgcgag
      121 gacgggaagg acagtttgct cggcgtgccg acgtgcaccg cgacgttcgg gtcgttcggc
      181 cgcttgcgcc gcaccacccg cttcaccgcg atcgacgcgg cgtgcgcggc caccgcgccg
      241 gccgacgcgg tcagccactc cttgcggcgt ggcttgtccc gcagcgcgcc gaggacgccg
      301 atggcgaacc agccgatgct gtgctcgccg aagtgcgaca tgccgcgcgc cgtcttgatc
      361 acgatcggct tcccgatcgc gccctgaacc cgttgcagca caacggtttc aggcgacgct
      421 gagggcgcgg gtttgtccgc gcgtgctccg ctcgtcagtt gtgaggtcag ctcagccatc
      481 gatcgatccg tccagcggcg caccctcggt gaggtgcggc gcgaccttgt tgtcgaatac
      541 ggacagcgcc gacccgatgg ccatgtgcat gtccaggtac ttgtacgtgc cgagccggcc
      601 gccgaagatc aggttccgct cgcgcgcctc ggccttggcc aactcgcggt accgctccag
      661 cttggcgcgg ttgtccgccg tgttgatcgg gtagtacggc tcgtcgtcgt ccttggtggc
      721 gaaccgcgag tactcgcgca cgatcaccgt cttgtccttc gggtgacggt cctcgcgctc
      781 ggggtggaag tgccggaact cgtggatccg ggtgtacggc acgtccgcgt cgttgtagtt
      841 catcaccgag gtgccctgga agtcgccggt gtcgacgacc tcgcgctcga agtccagggt
      901 gcgccagccc agccggccct cggagtagtc gaagtagcgg tccagcgggc cggtgtacac
      961 cgtcggcgtg ccggccggga tctggtcgcg cacgtcgaag tagtcgacgt tcagccgaac
     1021 ctcgatgttc gggtgctcgg ccatcttctc cagccacgcg gtgtacccgt ccttgggcag
     1081 accctcgtag gtgtcgttga agtagcggtt gttgaacgtg tagcgcaccg gaagccgggt
     1141 gatgatgctg gcgtccagtt ccttggggtc ggtctgccac tgcttgacgt tgtaacccct
     1201 gatgaacgcc tcgtagagcg ggcgaccgat cagcgagatc gccttctcct cgaggttctg
     1261 cgcgtccgcg gtgtcgatct cgctggcctg ctcggcgatc agcgcgcgcg cctcgtccgg
     1321 cgtgtgcgac ttgccgaaga actggttgat caggcccagg ttcatcggga acgagtagac
     1381 ctggccctgg cacttggcga agactcggtg ctggtagttg gtgaattcgg tgaactggtt
     1441 gacgtagtcc cacactcgct tgttggacgt gtggaacagg tgcgcgccat agacgtgcac
     1501 ctcgatgccg gtatcggggt ccggctcgga gtaggcgttg ccaccgatgt gcggccgacg
     1561 ctcgagcacg agcacgcgct tgttgagctg agtcgccacg cgctcggcga tcgtgagacc
     1621 gaagaagcct gatcccacga ccacgaggtc tgcctgccca agggggccgc cgtctgtttc
     1681 acccacgggc gctcagggta ccggcgtcac ctgtgcgctc cgaatcgcct gtgcctaact
     1741 accctctgtg acgctcttcg cgaggtcgcg gtcgagtgcc acccgtacga gtgatgccgt
     1801 gaaggcggcg agatcgtcgg cgtccacgag cctcgcgagc atgcgctcgt cggccggcag
     1861 acccacccgc gacgcgccgg cgagcacctt cttgtccaag taaggccgca gctcgggcca
     1921 cacttgctgg gcctcgcggc agaagatgtc cgcacccacc ggacccagcc ggggcacctc
     1981 ggtcagcagc gcgcggatct tgccctgatc accctcggca cgatcgcgca gccgacgcag
     2041 gtcaccacgg taggtatcga tcagcagacg cgcgccgtcg cccaacgcgg tggccgtgct
     2101 ctcgtcgtaa cggacgtagt gcgcgcggcc gagcgcgtcc acccgctgct gccaggtggc
     2161 gtccagcatc cgccgcggcg agccgaaccc cgagcgaacc agctcacgcg cggcggacac
     2221 cgcgatgtcg gccttgatcc tggtggagag caacaccgac aacaccagca agcggtacag
     2281 cggcgcggga gtgtcggtca ggcggatgcc cgcctgcgcg gcgtaggtcc gaccggccgc
     2341 ggccaacaat ccgcgcgcga tgtcccgctg tgccatggcc ggtggttacc ccgcaccacg
     2401 cgcgctacac ctcgagtgtg ggcagcacgt cggacgcgac gcgctccagc acggactcgt
     2461 cgccggcgaa gatcccctcg gccctcggcc acggcgccac cacgtcggtg aacccgagtt
     2521 cggccgcgcg gccgagcgtg tcggcgaaga actccatgct cgacagcacg taggccggtg
     2581 ccgcgtcgag ctggagaaat cgctggaccc cttgtgccgc aacggtttcg tcgaactttc
     2641 gcaccacggc ggcgaccgag ccccaccact ggtccatgcc gtcggtgtcc ccgccgcgcc
     2701 acggcccgcc ggtggtcgcc cacccggcac cgagcttggc ggccagccgc atgcccttcg
     2761 gcccgttcgc ggcgatcacg aacggcagcc gcggccgctg cacgcagccc ggcgcgttgc
     2821 gtgcctcgac ggcggtgaag tactcaccgc gaaaggtcgt ccgctcgccg gtcagcaacg
     2881 cgtccagcag cgtcacgaac tcggtgaacc gcgcgtgctt ggccaccggc gccagatcct
     2941 cgccacccat gacggtgctg tcgtagccgc cgccacccgc accgacgccg agcgtgaagc
     3001 gcccgtcgct gatgtcgtcc accgccatca gctcacgcgc gaacggcacc gggtgccgga
     3061 agttcggcga cgccacgaac gtgccgagcc ggatccgcga ggtgaccgtc gccgcggcgg
     3121 tcagcgtcgg caccgcgccg acccacggac cgtccaccag cgaccgccag ccgagatggt
     3181 ccatcgtcca ggcgtggtgg aagccgtact cctcggccgc ccgccatttc tcggcggcct
     3241 gccgccagcg gtactcggga aggatcgcga tgccgacgcg cacaggtgaa ctccttgccg
     3301 gataaggaaa ctcaggccac cgtatccgca ccggccgaca aagcgggtcg cccgttgatc
     3361 cgggcgcgga acgggcgcag gaacatcgcc gcggcgccgg cgatcaccac ctggctcgca
     3421 ccggacacca cgaacgtcca actcggactg tgcagccacc gggtgagcag cccgccgatc
     3481 agcgcgccca ccggcaccgc accccacacc agcgtccgcc acaccccgat cacccgaccc
     3541 agcagcgcgt ccgggacggc gaggtgccgc agcgtcccga cgaccacgtt gatcacgatc
     3601 accgcgccgc cgaagcagcc gagcagcacg gcggccagta ccggctggtg gaccaggccc
     3661 atcgccccga tcgccagccc ggacaccacg atgccggcca gcagcaccga ccagcggctg
     3721 aaccgggcga ccagacgcgc ggcgattccg gtgccggcga ggccccccac cccggcggcg
     3781 aacgcgaaca cgccgaacgc ggcctcggac agccgcaggt cctccagcgc gtagagcacg
     3841 agttgggcct gggcgaactc catggtcatg ccgagcaggc cggccagcac gatcagccgc
     3901 aggatcagcg ggctgccgcg cagccagcgc acgccgacgg cgaactccgc ccgcatcccg
     3961 cgcaccggct cgacatcggc ggcctgacgc gccgagtagc tgccggccag cccgacgagc
     4021 aggatcgcgg cgagtacgta ggccgcggtg ttgagcagga acgggaacgc ggcgaacacc
     4081 gcgaaggcga ggctgccgac ggggttgccg acgaagatct ggccgacgat ctcggtggac
     4141 tgcagcttgc tgttggcgct ctccaggtcg ctcttgtcga ccagcgcggg gatcagcacg
     4201 gtggccgcgc tgtccgcgac cgtctcggcc gtgcccatca gcagcgcggc gacgtacacg
     4261 atcggaatgg acgcggtgcc ggccagcacc atgatcgcca gcgcgccgac caccaccgcg
     4321 tgcagcacgt tcgcgaccag catcgccctg cgccggtcga cccggtcgag cagcacgccg
     4381 gccaccgcgc cgaacagcag ccacggcagc atctgcgcga cggcgagtcc ggcgatcaac
     4441 accgggtctc gtgtgagcat ggcggcgacc aacggaaacg cgacctggcc gatgccgtcg
     4501 ccgaggttgg acaatgcgct ggacgacagc aaccaattca accggttgtt ccccagagtg
     4561 cccaccgatt atcagacctc cccagcggtc ggagtacgac caccttcgca cgcgagggac
     4621 gcgacaacca caaggtcacc cgaccagact aacgggcgac aaacgctaaa tcattgattt
     4681 agtccacact ttgcttcttg agcgcgccgt gacgactccg tatcctcacg tacatccggg
     4741 tagtccagga tggaaccagt cctcgcccgc tgggaggttc ggaaaatgat cgaaaacagc
     4801 gactccggca gcatgacgat caagcatctc gcgaaggatg tccgcccggg aaagaagggt
     4861 acgcgcgtaa ttctcggcgc gtccgacctc cttcccggat gtgcgttcga agccgaagaa
     4921 ccgaatccgg ccgccggcgg ccgaccaccg ctcgccgagc actgacctga tggcgctgcg
     4981 acttcgtagt ggtccgggtc atgcgcacct gtctcgtctg tatgccgtgg cacgaccttt
     5041 cccggccgtc gctggccatc ggggtattgc gcacggcgtg ccggaacaag ggcatcgatg
     5101 cccctatcgg ctatcacggc ggactgcggt tcgccgattt cctcgccgag cgcactggtg
     5161 gcgctttctc gccgttcgaa tacaccgcaa tcgctgaggc gggattcaac cacgcgctcg
     5221 gcgactggat attcgccggc acgttgttcg gcgccgactt cggcatcgag cccatgcgcg
     5281 agtacgccga gcggcggaat ctgcgcatgc gatcggcctc ggccgcgcgg gaacacgcgg
     5341 acgagttcgt cgacctggtc gtggacgaac tcgccgcgct cgacgtcgag ttgatcggat
     5401 tcaccacgac gttcatgcag aacgtgccga gcctcgcggt ggccacccgg ctgaaacagc
     5461 gcaggcccga aatcgccgtg gtgttcggcg gcggaaattg cgacggcccc atggggatcg
     5521 cgctgcaccg cgaattcgag gcgatcgact tcgtgctgcg cggcgaatgc gacgagagct
     5581 acccgctgct ggtccagacc ctgtccggcg cgccggaccg gctcgacgcc gtgcccggcc
     5641 tgtgctggcg cacgccggcc ggtgccacgg tggcgaaccc gccgggacgg ctggtgccgc
     5701 ccgcgcacct ggtcgccccg gacttcgacg actggttcgc cgcgttcgag aagtccacgg
     5761 tcagcgagca ctcggcggcc gagctggtgg tcgagtccgc gcgcggctgc tggtggggcg
     5821 agcaccatca ctgcacgttc tgcgggctca acggcaccgc gatggcgttc cgcgccaagg
     5881 aacccgaggt gttcaccacc gaggtcgagc acctgatcac ccggcacgcg gtgctcgacg
     5941 tgatggtcgt ggacaacatc ctggagccga cctacctgcg caccgcgctg ccggagatcg
     6001 ccggccgcgg ctgggacctg cggctgcact acgaggtgaa ggcgaacctc acccggacgc
     6061 agatcgacgt gctggacgcg gccggggtgc gcagcgtgca gcccggcatc gagtcgctgg
     6121 tcgacgacgt gctggcgcgg atgaacaagg gcgtgcgcgc ggtgcacaac gtgcgcacca
     6181 tgcgcgacct ggagtcggcc ggcctgaccg tgtcgtggaa ctggctgtac gggttccccg
     6241 gcgagcgggc cgaggactac gccgccgtac tgccgcaact gcccgcgctg gtgcacctgc
     6301 agccgccgag ctgcaccgcg cggatcgagt tgaacaggtt cagcccgtac ttcgccgacc
     6361 cgtcgctcgg gttcccggtc cgcacacctg ccgaggtgta ccggcacgtc tacgaccggc
     6421 cgtccgagcg gctgcacgac ctggtgtacc tgttcgacac gccggaccgc gggctgaccg
     6481 ccgaacaggt cgcgccgctg aacgaggcgg tgcgccggtg gatcgacggc tacccggaca
     6541 gctcgctggt cagtacgcgt accgccgccg gcatcgtgat cagggaccgg cggatcggct
     6601 ggccggccgc ggatcacctg cttgacgacg agcggcagtg cgtcgcgtgg caggagctgg
     6661 agcacgggcg ctcggtgcgc gggctgctgc gccggctggc cgacgcgggc ctggcgtggg
     6721 acgaggacgg cctcaccggg tggctggacg agctgcgtgc gcacggcctg gtgttcaccg
     6781 agggcgggcg ctgggtcggc ctggccacct cgccgagggg cggctgagcc ggggtgcgca
     6841 ccgccgagcc gggcgagctg tggcccgacc atctcgtggt gcccgccgca gagctgacgg
     6901 tggcgcgggc gcgtggcgtg gtgcgccggg cgcaggcgtt cgcgcggcgg ctggtgatca
     6961 ccgatccgct gtccttcggc gagcgcgcgc cggccgtgct gcggctgctg accgacgcca
     7021 ccgcgcggcg ggtgccactg gagtggacgc tgggcggcga gccgccctgg ccggtgcgca
     7081 cgctcgtgca cctgacaccg ccggccggcc ggggcgagta cgcccggcgg tggcgcgacg
     7141 ggcaccgcgc cggcctgtgc tgctaccgcg tcgggccggg gttcgtgcgg gtgcgcgatc
     7201 tgcggccgga cggcgagcac cgggacgtgc tgatcaccgg cgcgctggcc aaccggttcg
     7261 tcgcgctggc cgaggatgcg gcggccgacg aggcgctgct ggccgatctg gtggacaccg
     7321 ggctcgcggt gcgcgtcgac gagggccacc acgtgctgcc gcatcgcctc ctgcgctggc
     7381 cgatcccgca caccgagatc tgaacctcgc gagtcccgcg ctcagacacc ccgagtcccg
     7441 cgccccgcat cgtgcacagc gcgggactca ccggtgcagg gcgcgggact cgcgaattac
     7501 tggacggtga gggtggggag cacgtcggag gcgacgcgtt cgaggaccga ctcgtcgccg
     7561 gcgtagatcc cgtccggccg cggccagtgc accaccacgt cggtgaaccc gaattcgccc
     7621 gcgcggccga ccatctccgc gaatgtgtcc actgtggaca atgagtacac cggcgaggcg
     7681 tccagcagca ggcaccgggc caccccggcg gaatcgcggc cctgctcgtg cagcgcctcg
     7741 tcgaaccgct cgacctgtgc gcgcagcgaa ccccaccagc cgtccacgtc gtcgcgcacc
     7801 gtgccggtgg tgatccaccc ctggccgaac cgcgcggcca gccgcatcgc cttcggcccg
     7861 gtgccggcga tcatgaacgg caccctcggc ttctgcgcgc agccgggcgc catccgcgcc
     7921 tggaccgcgc tgaaatgctt gccctcaaag gaagtcttcg gcgtggtgag cagcgcgtcg
     7981 gtcagctcga cgaactcggc gaaccgctcg gcgcgctccc gcatcgacgg ctccggcgtg
     8041 cccatcacca cgttgtcgta cttgtcctgg cccgcgtgcc ccgcgcccac gccgagggtg
     8101 aaacgcccgt cggagatgtc gtccagcgcg atcagcgacc gcgcgaacga caccgggtgc
     8161 cggaagttgg gcgaggcgac cagcgtgccc agccgcaggc tcgaggtgac cgtggccgcg
     8221 gcggtgagca ggccgatcga gtcgaaccac ggcccgtcca ccagcgatcg ccagccgaga
     8281 tggtcgtagg tccacgcggt gtggaagccg tattcctcgg ccgcacgcca tttcggcgcg
     8341 gcctcggacc accggtactc cggaaggatc accacaccca ctcgcacgcc ggccacgcta
     8401 cttcagcgcg acagtgccgg caggacgtcc gaggcgacct gctcgagcac cgactcgctg
     8461 cccgcgtaga tcccgtcgtg cctcggccac ggcgccacga cgtcggtgaa ccccagctcc
     8521 tcggcccgtc cgagcgcgtc ggtgaacgcc tccaggctgg acagcacgta ctgcggcgcg
     8581 gcgtccaggc ccaggtagcg gtcgatcggc acggtgcgcc cgtcggcctc caccgcgtcg
     8641 tcgaacttgc gcgcgacctc cgcgacaccg cgccaccacg cggtcatgtc gtcggactcc
     8701 tgccactcgc cgccgccgat cgtggcccag ccggtgccgt gccgcgcggc cagccgcatg
     8761 cccttcggcc cgttcgcggc gatcaggaac ggcactcgtg gcttctgcac gcaccccggc
     8821 gcgttgcgtg cctcgaccgc ctcgaagtac tcgccgctcc agctcgtcct gtcgtgtcgc
     8881 aggagctggt cgagcagctc cacgaactca ccgaaccgca cgcccttttc cttggcggac
     8941 aacggttctc cgcccatcac ccgctggtcg tagtcgccgc tgccgcccga tccgacgccg
     9001 agcgtgaacc gcccgtcgca cacgtcgtcg agcgtgatga tctcccgggc gaacgacacc
     9061 ggatggcgga actgcggtga ggcgacccag gttccgagcc ggatcttggt cgtcacggtg
     9121 gccgcggcgg tgagagtggg catcgcgccg aaccacgggc cgtccacgag tgttctccag
     9181 ccgaggtggt cggaggtcca ggcgtggtgg aagccgtact cctcaacggc tcgccatttc
     9241 ggttcagcga tccaccaccg gtgctcgggg tggatgagga tgcctacgcg cacgtgcccg
     9301 accgtagtgc caagtgtttg gtatcaacca acgtttggta cgtgaccgtg acccgggtcg
     9361 cttttgatgg cgatccgggt cacgaatcgc tcactccccg tacaactttc ctgtgtacag
     9421 cactgtcccg cgcgcgtcgt acacagtgca cacgattccg ttcaaatcgg gcatttgcgc
     9481 cctttccaac gtgttctcct tcggcagcaa caatgcggcg aaagtcgcgt cgtgcacgtc
     9541 cgccgacacc ggcggccgcc ccggcacggt gatctccatc cgacgcgcct ccgggagcag
     9601 cgtgccgccg accgcgatct tcccgccgat caccggcgga agggccagca ccctcggctt
     9661 cgccggcgcg tccggtgtcg cccgcttctc ttggtacaca ccgaattgcg ggtgacgctt
     9721 cgcgccctgg tcggtgcacg aggcgacccg gtccccggcc accgcgaacg cgacaagctc
     9781 gtcaccggag ccggccgcgg caccgggatg ccagctttcc gggtccggca cgccgttctg
     9841 gctcatggcg gccgcgatgc acgcgtcgag cgccttgcca cgctcggacg tgcggtccgg
     9901 tggtgggccg atcggctcgg tcacggccac ggccggtggc catggggtgg gcacccgtag
     9961 ctcgccgtca gccgtgatcg cggtgacctt gcccggccga ggagtgacgg ctgaggtgaa
    10021 aacgaacagg cggtccttgt gccacgccgg cccgccgccg gacatgccgt cgccctccgc
    10081 tcggaagttc ggctcgcgcc acgcgggatc gagcactccg gcgagtgtcc cttgtggact
    10141 gatcagcgcc accgaggtgc gcccgcgcga caggtgggcc ggtgccgcgt tcgggtccgt
    10201 cagcgtcacc ttcgtcctgg tggtctcgca gaacagcggc ttgccgtccg cgtgcgcggc
    10261 ggtgaccgtc gtgccacgga agtcgtccac ggtgaacacc ggcaaccacc gcgcacggtc
    10321 cggcagccgc gccgccgcac ccgtccgcag cgccgccgcc caacagcggt ccagttgcac
    10381 cttggccttg accggatcca gcggcggctc ggcgtagttc ggcggcgacg acgtggtggt
    10441 agtcggcttc gacgtcgacg gcgacagcac cggtgcggcc gtgtcctcgc cgttgccgaa
    10501 cggctgcgcg atcgccacgg cgccggccgc gacagccacc gcggccgccg ccgcgatcgg
    10561 cgcgtaccag cgcgtactcc ggccgtcgat gccacgaaaa accttgtccc gcaagcgatt
    10621 ccgcacatcg tccgggatcg tcctatccgg acggtcgaag tcgtcgatgc tcattccgat
    10681 tcctccaacg tctgacggag tcgtgctcgc gcgcgggaca ggtggccgcg cacggtgccc
    10741 tggtcaattc cggtcacctc ggccgcctcc gcgggggcga gaccggcgac caggcacagc
    10801 tcgaccaccc ggcgctgcgc gggcggcagc gcggtgatcg cgtcggccgc gcgccgcgct
    10861 ctggcctcgt cgtccacccg ggcggccaca ctgtccgcgt ggtcggcgac cgcgaccggc
    10921 tcggacacct tcttcaccag tcgcagccgc cgcagcagcc cgcgacgctc gtcccgggcg
    10981 aggtttccca tcaccgtgta cagccagggc agcgcgctgt cccgcacgag ggtgatctcg
    11041 gctctgcgcc gccacgcggt gaggaacgtg gccgaggtga ggtcctcggc ctgggcccac
    11101 gagccggtca gccggtacgc gtggttccac agccgctgca cgtgccgctg gaagagcacg
    11161 ccgaagtcgg ccttgccgcc gtcggccgca cgccgccaca gctcggcatc ggtgtgctcc
    11221 gcggcctcgg gtaccggcaa ggtcaacacc cctggagatc tcgcgttggt ggtcacgtct
    11281 gtcagtggcc ggcagcgcgc gaatcgttgt gagtctcgcg ccattctccg acggcgatgc
    11341 tggacgggcg tgccaatcgc gagaagataa ccccgtgcag aagccgagac tggtcgcatc
    11401 cgacgtggac ggaaccctgc tcgatccgat ggagcgggtc acctcgcgca ccgagcgcgc
    11461 gatcgccaga gtgaccggat cagggacgcc gttcgtgctg gtcaccggcc gaccgccgcg
    11521 ctggatcccg tccgtggccg acgccgccgg gctggacggc tacgcggtgt gctccaacgg
    11581 cgcggtgctc tacgacatcg gcgcggacca ggtgctctcg gcgaccgaga tcgacccgat
    11641 ggtgctgcac gacgtcgcgc acgcgctgca acgggtgctg ccggggatgt cgatcgcggt
    11701 ggaacgggtg accgcgtcgg tgcgccgcga gacgttcctc gagcacctgg ggcgcacgtt
    11761 catgcacgag ccggcgttct ccaacccgtg gggcgacaac gagcacaacg tcgcgccgcg
    11821 cgccgag
//
