LOCUS       NZ_SGWQ01000001        10390 bp    DNA     linear   CON 10-APR-2024
DEFINITION  Herbihabitans rhizosphaerae strain DSM 101727 Ga0310478_101, whole
            genome shotgun sequence.
ACCESSION   NZ_SGWQ01000001
VERSION     NZ_SGWQ01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Herbihabitans rhizosphaerae
  ORGANISM  Herbihabitans rhizosphaerae
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Herbihabitans.
REFERENCE   1  (bases 1 to 1096878)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1096878)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 226.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004216555.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 19:27:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,690
            CDSs (total)                      :: 6,628
            Genes (coding)                    :: 6,561
            CDSs (with protein)               :: 6,561
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 13 of 67
            Pseudo Genes (incomplete)         :: 59 of 67
            Pseudo Genes (internal stop)      :: 5 of 67
            Pseudo Genes (multiple problems)  :: 9 of 67
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:31:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 929047
            Orig. end                         :: 939437
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SGWQ01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5390](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5390
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1029..1364)
                     /locus_tag="EV193_RS04640"
                     /old_locus_tag="EV193_101940"
     CDS             complement(1029..1364)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EV193_RS04640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EV193_101940"
                     /product="hypothetical protein"
                     /protein_id="WP_130342644.1"
                     /transl_table=11
                     /translation="MPVVTIGRVSALVLSGGTFAFLFVNNSWRSDNLFLVPDLILCAVL
                     VAAALTPRPRVQRALLFAFGLTAGVLMTSVASYAVDGRLGVASLIGAVLSVVAAALLAR
                     SRQPSAA"
     gene            1462..2076
                     /locus_tag="EV193_RS04645"
                     /old_locus_tag="EV193_101941"
     CDS             1462..2076
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /locus_tag="EV193_RS04645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_101941"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_130342645.1"
                     /transl_table=11
                     /translation="MARSGRLTPAAIVTAALRLGDREGAEAMSMRRIAAAIECDPMALY
                     RHFTNREALLDAVADAALTDAQDPGPDTPWSERLHTIMAAIRSSALRHPGIAAHIAARP
                     PLGENGRRLGAGIFAALTEAGLAPPDVVRASQTLIAHLAAALAMSVRAGRRDARWEQVS
                     QTITQSPGAPPGEHLFIVGSDEQFEYGLRLLVNGIRAEAAG"
     misc_feature    complement(1498..1510)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            complement(2176..2661)
                     /locus_tag="EV193_RS04650"
                     /old_locus_tag="EV193_101942"
     CDS             complement(2176..2661)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /locus_tag="EV193_RS04650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_101942"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_165401223.1"
                     /transl_table=11
                     /translation="MPVMSDESFGPGSAPSEADIDAVFVWTAIRVGRLLERMAAARLAD
                     TGHGLTQVQFGVLAYLGARPGMSQSELARAIQVRPQSAHETVTAMIDEGVLERVGPGGR
                     GRPAALRLTDHGYALLSRTWPAVTGISATDLGLTPDDAATLNGLLHTVLHTPATRSR"
     misc_feature    2726..2741
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.78"
                     /tool="antismash"
     gene            2727..3968
                     /locus_tag="EV193_RS04655"
                     /old_locus_tag="EV193_101943"
     CDS             2727..3968
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /locus_tag="EV193_RS04655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_101943"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_130342647.1"
                     /transl_table=11
                     /translation="MRIIISGAGVGGLATAQGLLAAGHEVVVLERGELQQAGPSGYRLR
                     LDSRAMTVLARLLPASLFTALLASGSGRAVNQRLTYLDQRLRPLGSTREGDRDVLTIGR
                     GPLLRLLAHGIADHVREHRETRSFQITPDHVSITTTDGQTETGDVVIVAEGAHSPLRRQ
                     LLGDTGGRVLDAGMVSGRVPGRAADLGLTGIADALRHGYALATGRGGFGMFLSAHDPVT
                     GPAVDPTVAGPIQPDLEDPYLIWALLGPTGRLPGTDAGQSVLRAAVEDLTRAWHPDIAH
                     LPRLTDPVSLRRVALYAAPPVPAWPTTAVTLLGDAVHVVPPTGGIGASTAIRDAGHLVD
                     ALTGIGDRPALLNALHQYEQTMRDYADDIVTGSIAPLRWQHRLRHPLVYTAVTATRATT
                     FALTGRSLPLPSRN"
     misc_feature    3951..3953
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3986..4639
                     /locus_tag="EV193_RS04660"
                     /old_locus_tag="EV193_101944"
     CDS             3986..4639
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /locus_tag="EV193_RS04660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_101944"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_207222484.1"
                     /transl_table=11
                     /translation="MHAFQAGHANAYLWRHGTTATLIDAGEVGHDTTVLDVLRHLGLDL
                     TRILITHWHHDHTGAAAALADRTGAQVYAGRADAPVIRGERDGVPPMLTAAEEPLMASI
                     AGTVEPAPACQVHRELHDGDTLDEHAVIIGTPGHTPGSIAVHLTTERVLLTGDISAHQN
                     GQVILGPFNTDREQAKRSLRRLTTIDVDAVGFGHGTPVTENGKATLTTWTDPLG"
     gene            5001..5390
                     /locus_tag="EV193_RS04665"
                     /old_locus_tag="EV193_101945"
     CDS             5001..5390
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="EV193_RS04665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_101945"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_165401224.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.8e-08,
                     bitscore: 30.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKAAKAASIASLTLFMLTGAGSVAQATPIHGGSAVTDGPEVQS
                     CDPADGGTWCHGTSGAWPPGWQNCFSNYINNEFWHSASVAMGGGTNKKYAAPGYWANAN
                     VVGGIALVCNSFWDNRVDPPPPSGS"
     gene            5532..6818
                     /locus_tag="EV193_RS04670"
                     /old_locus_tag="EV193_101946"
     CDS             5532..6818
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EV193_RS04670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EV193_101946"
                     /product="hypothetical protein"
                     /protein_id="WP_130342650.1"
                     /transl_table=11
                     /translation="MNRQMRRARSRSFFRVAIAVPLVLKVACGAALIVLTVALWRQFVD
                     LDQHPEPTSPWGNAQNYAVFHPRLMGDDRREFETGGDASTVAEARDLYPVLDKAGGLYV
                     DSVNYQAGAPNDPSSPWPARPIRVNGNFLQQYPILDASGKKIAVPDDEKAWVVAVPERF
                     KPREAQFKEYLRSIRASASLAEARVVDEKALQRFAGQEVRIIWTASGQEVFSFDPRVNP
                     DRGNMITDPVVEIMTPANSLTVDRLNSITGGLDAGLKIRVDGDPAAVSAQFAPLLRQLR
                     LDDNLRHLVNVPAPAGTQASESRDPTTQSVALAVGALLVMVLLQVTIVVTGSDRLRSQR
                     TARRLLGSHRELLLVSGATWLVQTLIAAVVVWHGTQATLIPGVEVSPYSQTPKLLAVAV
                     ISLAIEALLLIVTARIMERRNAAKPLKQL"
     gene            complement(6827..8554)
                     /locus_tag="EV193_RS04675"
                     /old_locus_tag="EV193_101947"
     CDS             complement(6827..8554)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /locus_tag="EV193_RS04675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_101947"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_165401225.1"
                     /transl_table=11
                     /translation="MEREGRPVDVPSGRQRSLLAILALSPNLTATTDTVIRRLWGADAP
                     PGAPTTVRGYVKRLRRLLDVDHAPSVIRTVARGYQLDLAPDDVDVVRFTELVRDAQTSD
                     RPGGESELLRQALSLWRGPALSDVDCDALHREALPALREQYLRSYGRFLELELPRRPED
                     VITELRGLVTEYPLREGLWTQLISALHRCGRQAEALATYADCREVLADELGAEPGATLR
                     ALHQRVLNGDTATDLIVPAPRQPDYRTPHQLPPVPQSFAGRAAEAGVLDRMLDHGRGDT
                     PIAVVDGPAGVGKTALAVHWAHRNRHRFPGGEIYIDLQGYSGGQPLAPTVALRRLLNGL
                     GETTPPTADLATLAAAFRSVTTHRGALIILDNAKTSDQVRQLLPGAGNAVLTTSRSQLR
                     GLVARDGASRLRLGQLDRADSAELLTRIVGEHRCGTEKNAVDELVEHCGGLPLALRLLG
                     EYLTRHPDLPLQRIAALLGNPVNRAGVLRSHEESTDLATVFSWSIQALPGEARRLLRLL
                     AASDVSEIESEAAMHLAGMRHDDVLRWLDTLVEANIVQQPAPDVYTLDALTRTLTRMR"
     gene            8729..9340
                     /locus_tag="EV193_RS04680"
                     /old_locus_tag="EV193_101948"
     CDS             8729..9340
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /locus_tag="EV193_RS04680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EV193_101948"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_130342652.1"
                     /transl_table=11
                     /translation="MGNREDLLAGAKRCLLENGYARTTARDIATTSGVSLAAIGYHFGS
                     KEALLHAALTEIVGAEIGEEITDAIKRTDRRDTAIQRFETMWRQLIESFPKHRPLLVAS
                     MERLAQLEHVPAVRESLADGQRRSIDTIAELFRDTHHLDDQDAHAVSALSYALLNGIMF
                     MWLTDPETTPDAHTLRRALTALAETPSAIPTTEPPNGDVS"
     misc_feature    10244..10258
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.37"
                     /tool="antismash"
ORIGIN
        1 gcgtcgccca gtccggcgac ctgagcagcc gagtgaaccg gccgcgctcg tcctcggtca
       61 ggttggcgat caccgcggtc ggcggctggc ggtactgggc ggtcagcgcg cccagacggg
      121 tccgcgcggc atggtcgccc gtggcctccg tgtacacccc gagcgcgtgc gcccgcgaca
      181 ccgactcgat cacgaagaac aggtccgagc tgaccacgtg ctcgaacgcg accgcggcgt
      241 cgtcggccga ggcggccagc cgcttggagc cgatcccgat cacgtccagc acctggttga
      301 agaagtcgaa accggcaagc gcgggcgtcg cccccgagtc gagcgcggca cgcgtctcgg
      361 gcaccgtccg cagatactcc tcgcccttga cgacctgagg tcgcagctcc ggcgaatgcc
      421 cgtcggccag accacgcgcg tactcgatga cctccaccag ttcggcgtcg acgacgcgcc
      481 gccgctcggc cagccgcggc cggtcggcct ccgagccgcc cagcaccgcc atcgtcagcc
      541 ggcgctcctc ctgccgcgag gcgacgtacc tgccaaccac gggaagaccg gaccgacgcg
      601 cgtccgcgaa ctccagcgac gcgccaccct gtcggatcag gtagaccgac agcgcaccac
      661 cgaacagcaa cacgaccagg cacggaatca acgcgatccg gagcacccgc gctctgatcg
      721 accgtgcccg acgggatccg acccgactcg ccatgatgca gcaccgcacc ttcacccacc
      781 gacgacatga gccggccggc tacctggtca catccaccgt cgcagatggc gtggcacagg
      841 tcacaggccc ggctggttcc gcaggacgct atgtaccgcc gccagtcgga gcatcgggag
      901 aatgacgtat ccaccgacgt agacgccgat cagaccttcc ggcagaagga cttcgagagc
      961 gaatcctcgg gttgtctcgc ggagaggtga aacccgttgt gccgcaacgg tttcagcgca
     1021 cactcccatc atgcagcgct gggttgacgt gaccgcgcga gcagcgcggc ggcgaccacc
     1081 gacagcaccg ctccgatcag gctcgcgaca ccgagccgcc cgtcgaccgc ataggacgcc
     1141 accgacgtca tgagcacgcc cgcggtgagc ccgaacgcga acagcaaggc gcgctgtacc
     1201 cgcggccgtg gtgtcagggc ggcggcgacc aacaccgcac acaagatcag gtcgggaacc
     1261 aggaacaggt tgtccgaacg ccacgagttg ttgacgaaca ggaacgcgaa cgtgccgccg
     1321 ctcaacacga gcgccgacac cctcccgatg gtgacaacag gcatttcacc ctcctcagaa
     1381 cgctgaggtc tacgacgtag accgccctcg cgtcgagtgt ctacgctgta gactgaaggt
     1441 gttctcagca gaaaggcgca catggccaga tccggacgcc tcacgcccgc ggcgatcgtg
     1501 acggcggcac tgcgactcgg cgatcgggaa ggcgccgagg ccatgtccat gcgccgaatc
     1561 gcagccgcca tcgaatgcga tccgatggcg ctctaccggc acttcacgaa tcgcgaggcg
     1621 ctgctggacg ccgtggccga cgcggcgctc accgacgccc aggaccccgg cccggacacg
     1681 ccgtggtccg aacggctgca cacgatcatg gccgcgatcc gatcgtcggc gctgcgacat
     1741 cccggcatcg ccgcgcacat cgccgcccgg cccccgctgg gcgagaacgg gcgtcggctc
     1801 ggcgccggaa tcttcgccgc actgaccgaa gccggtctcg caccacccga cgtggtgcgg
     1861 gcatcgcaaa ccctcatcgc ccatctcgcc gcagcgctgg cgatgagcgt gcgagccggt
     1921 cgccgggacg cacgctggga acaagtcagc cagaccatca cacaatcacc cggtgcgcct
     1981 cccggcgagc acctgttcat cgtcggctcc gacgagcaat tcgaatacgg actgcgccta
     2041 ctcgtcaacg gaatccgcgc cgaagcggcg ggctgaacga caccatcgcc ggtcagcggg
     2101 gaaaccgggt gacctcgatg acccgggctt ctatcgatcg tcctgtgacc acccgacggg
     2161 cggcgggttc gggcactatc gggatcgcgt cgccggcgtg tgcagcacgg tgtgcagcag
     2221 gccgttgagt gtggcggcgt cgtcgggtgt gagcccgagg tcggtggcgc tgatgccggt
     2281 gaccgccggc caggttcggg acagcagcgc gtatccgtgg tcggtgagcc gcagggctgc
     2341 ggggcgtccg cgcccgcccg ggccgacgcg ttcgagcact ccctcgtcga tcatggcggt
     2401 cacggtctcg tgggcgctct gcgggcggac ttggatagcc ctggccagtt cggactgact
     2461 catgccaggt cgggcgccga ggtaggccag cacgccgaat tgcacctggg tcagtccatg
     2521 cccggtgtcg gcgagccggg ccgcggccat gcgctccagc agccgaccga cgcggatggc
     2581 ggtccacacg aagaccgcgt cgatgtcggc ctcactcggg gccgatcccg gaccgaacga
     2641 ctcatcggac atcacaggca tcgccatcct tgcggaaaca ggtacctgca tcctatggtt
     2701 acaggtacct gcaactagga gatgcgatgc ggatcatcat cagtggtgcg ggcgtcggcg
     2761 gtctcgccac tgcccagggc ctgctcgccg cgggtcacga ggtggtcgtg ctggagcgcg
     2821 gcgaactgca acaggccggt ccgagcggct atcggttgcg gctcgactcg cgcgcgatga
     2881 ccgtgctggc gcggctgctg ccggcgtcgt tgttcaccgc actgctcgcc tcgggcagtg
     2941 gtcgggcggt caaccagcgc ctgacctacc tcgaccagcg actgcgcccg ctgggcagca
     3001 cgcgcgaagg cgaccgggac gtgctgacca tcggccgggg gccgttgttg cgactactcg
     3061 cccacgggat cgccgaccac gtccgtgaac accgggagac ccgctcgttc cagatcacgc
     3121 ccgaccacgt gtcgatcacc accaccgacg ggcagaccga gaccggcgat gtcgtgatcg
     3181 tcgccgaggg agcccactcc ccgctgcgcc gccaactgct gggcgacacc ggtggccgcg
     3241 tgctcgacgc cggcatggtc agcggccgtg tccccgggcg cgccgccgac ctcgggctca
     3301 ccggcatcgc cgacgcgctg cgccacggct acgcgctggc caccgggcgc ggcgggttcg
     3361 gcatgttcct cagcgcccac gacccggtca ccggccccgc cgtcgacccc accgtcgccg
     3421 gcccgatcca gcccgacctg gaggaccctt acctgatctg ggcgctactc ggcccgaccg
     3481 gtcgccttcc cggaaccgac gccggccaga gcgtcctgcg cgccgccgtc gaggacctca
     3541 cccgagcgtg gcaccccgac atcgcgcacc tgccgcgcct gaccgatccg gtatcgctac
     3601 gacgtgtcgc cctctacgcc gcgccgccgg tgccggcctg gcccaccacc gcggtcaccc
     3661 tgctcggcga cgccgtccac gtcgtcccgc cgaccggcgg tatcggcgcg agcaccgcga
     3721 tccgcgacgc cggacacctc gtcgacgcgc tcaccgggat cggtgatcgg ccggctctgc
     3781 tgaacgcgct gcaccagtac gagcagacca tgcgcgacta cgccgacgac atcgtcaccg
     3841 gctccatcgc ccccttgcgc tggcagcacc gtctgcgcca ccccctggtc tacaccgcgg
     3901 tcaccgcgac ccgcgccacg acgttcgcac tcactggaag gagcctccca ttaccgtccc
     3961 ggaactgacc ccgctcagcc cgaacgtgca cgctttccaa gccggccacg ccaacgccta
     4021 cctgtggcga cacggcacga ccgcgacgct gatcgacgcc ggcgaggtcg gacacgacac
     4081 caccgtgctc gacgtgctgc gccacctcgg gctcgacctg acccgcatcc tgatcaccca
     4141 ctggcatcac gaccacaccg gagccgccgc cgcactcgcc gaccgcaccg gcgctcaggt
     4201 ctacgccgga cgcgccgacg caccagtcat ccgcggcgag cgggacggcg ttcccccgat
     4261 gctcaccgcc gccgaggaac cgctcatggc cagcatcgcc ggaaccgtcg aaccggcccc
     4321 cgcctgccag gtccaccgcg agttgcacga cggcgacacc ctcgatgaac acgccgtgat
     4381 catcggcacg cccggccaca cccccggcag catcgccgtg cacctcacca ccgaacgcgt
     4441 actgctcacc ggagacatct ccgcccatca gaacgggcag gtcatcctcg gccccttcaa
     4501 caccgaccgc gagcaagcca aacgatcgct gcgccggctc accaccatcg acgtcgatgc
     4561 tgtcggcttc ggccacggca ctcctgtcac cgagaacggc aaggccacgc tcaccacctg
     4621 gaccgacccg ctgggctgat caccagccgg caaagctggg cggctacgag cagtccaggg
     4681 attcctggtg aattcctgcg cctgcgagat cgaagacggt gaacgtacgt gccgcttcag
     4741 cggtgatcgc acaagtcccg tgccacgtga ggacaacgcg ggtgccacgc gactgccacg
     4801 tccaccggac aaactgaagt tcgtcgcgcc atacgagcct cgacgtttcc acgacgtgga
     4861 tacagagggc tggcgtgcaa ctcacaaagt gggtcgaacg gcgtgtcttc acgatggcta
     4921 ctacctacgc cggacacgag gatctccgtt cgattcaaca agggaacatt gccgataggg
     4981 gacgaatcag tatgtcacca ttgaagaaga aagccgcgaa ggcggcatcg atcgcttcgc
     5041 tcacactctt catgctcacg ggagctggct cggtcgcgca ggccaccccg attcacggcg
     5101 ggagcgcggt taccgacggt cccgaggtcc agtcctgcga ccccgccgac ggcggcacct
     5161 ggtgccatgg cacctcgggc gcatggccac ccggctggca aaattgcttc tccaattata
     5221 tcaataacga attctggcac agcgcatccg tagccatggg aggtggcaca aacaagaagt
     5281 atgccgcacc aggatactgg gcaaacgcca acgttgtggg cggaatcgct ctcgtgtgca
     5341 actccttctg ggacaacagg gtcgatccac caccacctag tggatcgtag cagccagagc
     5401 gcacgatgag tcagtatcgg acccgaccat cgggtccgat actggcgcgc gccttttgac
     5461 atcaattcct cacgtctatc aagccttcgc gatcaccaac gagccgcaaa catgacaaaa
     5521 ggagcttcgc aatgaatagg cagatgcgcc gcgcgcgaag caggtccttc tttcgcgtcg
     5581 cgatcgcggt gccgctcgtg ttgaaggtcg cgtgcggggc ggcgctgatt gtgttgactg
     5641 ttgccctgtg gcgacaattc gtcgacttgg atcaacaccc ggagccgaca tcaccctggg
     5701 ggaacgccca gaactacgcg gttttccatc cacgcctcat gggggacgac cggcgagagt
     5761 tcgaaaccgg cggcgacgcc tcgacggtcg cggaggcgcg cgacctgtac cccgttctcg
     5821 acaaggcggg cgggctctac gtcgactccg tcaactatca ggctggcgcc ccaaatgacc
     5881 cgtcgtcgcc gtggcccgcg cggccgatcc gggtgaacgg caactttctc cagcagtacc
     5941 cgatcctgga tgcgtcagga aagaagatcg ccgtcccgga cgacgaaaag gcatgggtgg
     6001 tcgccgtgcc agagcggttc aaaccacgag aagcccagtt caaagaatat ctccggagca
     6061 tccgagccag cgcctcgctc gcagaggcga gggtcgtaga cgagaaggcg ctgcagcggt
     6121 tcgccggcca ggaggtccgg atcatttgga ccgcttcggg gcaggaggtg ttcagcttcg
     6181 accccagagt caaccccgat cgcgggaaca tgatcaccga tccggtcgtg gagatcatga
     6241 cgcccgcgaa cagccttacg gtcgaccggc tgaactcgat caccggtggt ctggacgccg
     6301 ggctcaaaat ccgcgtcgac ggcgacccgg cggccgtgtc cgcccagttc gccccgctgc
     6361 tgaggcaact ccggctcgac gacaatctgc ggcacctggt gaacgtgccc gctcccgcag
     6421 gtacgcaggc cagcgagtca cgcgatccca cgacacagtc cgtcgcgctc gcggtcggcg
     6481 cgcttctcgt catggtcctg ctgcaagtga cgatcgtcgt caccggctct gatcggctgc
     6541 gcagccagcg caccgcacgc aggctgctcg ggtctcaccg ggagttgctc ctcgtgtcgg
     6601 gtgcaacgtg gctggtgcag accctcatcg ccgcagtcgt cgtatggcac ggaacacagg
     6661 cgacattgat ccccggcgtc gaggtgagtc cgtattccca aacgccaaaa ctgctcgcgg
     6721 tagccgtcat ttccctggcg atcgaggctc tacttttgat tgtcaccgca cgcatcatgg
     6781 aacgccggaa cgcggcgaag ccattgaaac agctgtagcg gctaattcag cgcattcgcg
     6841 tcagggtgcg ggtgagtgcg tcgagtgtgt acacatcggg tgcgggctgt tggacgatgt
     6901 tcgcctcgac cagggtgtcc agccatcgga gcacgtcgtc gtggcgcatg ccggccagat
     6961 gcatcgccgc ctcggactcg atttccgaca cgtccgaggc cgccagcagc cgcagcagtc
     7021 gtcgcgcctc gcccggcaga gcttggatcg accaggagaa caccgtcgcc aggtccgtgc
     7081 tctcctcgtg actacgcagc acgcccgccc ggttcaccgg gttcccgagc aacgcggcga
     7141 tccgctgcag cggcaggtcc gggtgccgcg tgaggtattc gccgagaaga cgaagggcca
     7201 gcggcagccc accacaatgc tcgacgagct cgtcgaccgc gttcttttcg gtaccgcacc
     7261 ggtgttcgcc gacgatgcgg gtcagcagct ccgccgagtc cgcgcggtcg agttgtccga
     7321 gtcgcagccg gctggccccg tcccgggcga cgagcccacg cagctggctg cggctggtgg
     7381 tcagcacagc gttgccggcc ccgggcagaa gctgccgcac ctggtcggag gtcttggcgt
     7441 tgtccagaat gatcagcgca ccgcggtggg tcgtgacact gcggaacgcg gcagcgagcg
     7501 tcgcgaggtc ggcggtcggc ggcgtggtct cacccaggcc gttcagcaat cgccgcaacg
     7561 cgacagtcgg cgccagcggt tgaccaccgc tgtacccctg cagatcgatg tagatctccc
     7621 caccgggaaa tcggtggcgg ttgcgatggg cccagtgcac ggcgagcgcc gtcttgccga
     7681 cgccggcggg cccgtccaca accgcgatgg gtgtgtcacc tcggccgtga tccagcatcc
     7741 ggtcgagaac accggcttcc gcggcgcgac cggcgaagga ctgcggcacg ggcgggagct
     7801 ggtgtggtgt ccggtaatcg ggctgccggg gagccggaac gatcaaatcg gtcgcggtgt
     7861 cgccgttgag gacccgctga tgcagcgcgc gaagggtcgc gccaggctcg gctccgagtt
     7921 cgtcggccag cacctcgcgg cagtccgcat acgtggcgag agcctcggcc tggcgcccgc
     7981 aacggtgaag ggccgagatg agttgcgtcc acaggccctc gcggagcgga tattcggtga
     8041 ccagcccacg caactcggtg atcacgtctt ccgggcggcg aggcaactcc agctcgagaa
     8101 accgtccgta tgaacggaga tactgttcgc gaagtgccgg aagagcctcg cggtgcagcg
     8161 cgtcacagtc cacatcagac agtgccggtc cacgccacag ggacaaggcc tggcgaagca
     8221 actccgactc gccaccaggc cggtcagaag tctgcgcatc acgcaccaac tcggtgaacc
     8281 gcacgacgtc gacatcgtcc ggggcgagat cgagctgata gcccctggcc accgtcctga
     8341 tcaccgatgg ggcgtggtca acgtcgagca agcgccgtag ccgcttgacg tagccgcgga
     8401 ccgtggtcgg cgcccccggc ggggcgtcgg ccccccacag gcgcctgatg accgtgtccg
     8461 tcgtagcggt caggttcggg ctgagcgcga gaatcgcgag cagcgatcgc tgccgacccg
     8521 acggaacatc gaccggccgg ccttcgcgct ccacttcgaa cctgcccaat agacggatct
     8581 cgactggtgt cactgatccc ccgacctgtg atcgtcaccc ggagccggcg cgggcctcgc
     8641 cctgatcgcg ccgccactaa ggacaggcta cgcatgactt agacatatgt gcaagacata
     8701 tgtctaagtc gcttgctacg ctgcgcccat gggaaaccgg gaggacctgc tcgcgggcgc
     8761 gaagcgctgc ctactggaaa acgggtacgc gcgtaccacc gcacgcgaca tcgcgacgac
     8821 ctccggtgtc agcctggccg cgatcggcta ccacttcggc agcaaggaag cgctgctcca
     8881 cgccgcgttg accgagatcg tcggcgcgga gatcggcgag gagatcaccg atgcgatcaa
     8941 gcgcaccgac cgccgcgaca ccgccatcca gcgcttcgaa acgatgtggc ggcaactcat
     9001 cgaatcgttc ccgaaacatc gcccgctgct cgtcgcgagc atggaacggc tcgcacaact
     9061 ggaacacgta ccggccgtgc gcgagtccct ggccgacggc cagcggcgat caatcgacac
     9121 catcgcggaa ctgttccgcg atacccacca cctcgacgac caggacgccc acgccgtctc
     9181 cgccctctcc tacgcactgc tcaacggaat catgttcatg tggctcaccg acccggaaac
     9241 cacaccggac gcgcacaccc tgcgccgtgc cctgacagct ctcgccgaaa caccgagcgc
     9301 cataccgacg accgagcccc cgaatgggga tgtcagttga acggcgcgct cattccgcgg
     9361 cgccacagga ttgccacgcc ggtgccacac agaccgatta acgtcgccct cgatctcgga
     9421 tgagatcccg atggccctcg cgctgacgtc agtcgaccct gagcaaccag gaattttgag
     9481 gaacgagtaa tggtgcggat tctcatcgtt ggcgcgggtg tggccggcca cgccctggcc
     9541 tactggctcg gtcgccatgg gttccagccc actgttgtgg aacgtgcgcc cgcgatacgc
     9601 gaaggcggct acaaactcga tatccgtggt gcggcgatgg aagtcatcga ccgcatggaa
     9661 ctgacggacc aaatacggaa acacagcacc ggcatccgtg gtgcgtccta tgtcaacggt
     9721 gaaggcaccc gggtggcgag cctgcccgcc gacgtgttcg gtggccgtga cgacaccgtg
     9781 gatgtcgaga tcatgcgtgg agagttggtg cggctgcttc acgagagcac ccaggtcgat
     9841 tacctcttcg gcgagtcggt ctcggcgatc cggcaacacg gcgacgccgt ggaggtgacc
     9901 ttcgccagcg gccgggctcg cacgttcgac cttgtggtcg gcgcagacgg gctgcactcc
     9961 gcggtgcggg cgctggcctt cggagccgaa gacgagtttc ttcggtacct gggctatcac
    10021 tgcgcggtgt tcaccgcgcc caaccacctc ggcctcgatc gctgggagtt gctgcacgcg
    10081 gtgcccggca aggtcgtcaa cgtgtacggc acccaggaca gcgaggacgt caaggcggcg
    10141 ttcttcttct cctcgccacc tctgaaatac gaccaccgcg acatcgccca gcagcagcgg
    10201 ctgctgctgg atacctaccg tggtgtggga tgggaagcgc cacggttgct gtcggccatg
    10261 cgtgacgcgc cggacttctt cttcgactcg atcagtcaga tccgcatgcc gacctggtcc
    10321 agcggccgcg tcgccctggt cggcgacgcg gcatactgcc cgtcacccct gtccggacaa
    10381 ggcacgagcc
//
