LOCUS       NZ_RYEV01000008        10852 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Peristeroidobacter soli strain JW-3 Scaffold7_1, whole genome
            shotgun sequence.
ACCESSION   NZ_RYEV01000008
VERSION     NZ_RYEV01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Peristeroidobacter soli
  ORGANISM  Peristeroidobacter soli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Steroidobacterales; Steroidobacteraceae; Peristeroidobacter.
REFERENCE   1  (bases 1 to 418920)
  AUTHORS   Huang,J.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2018) College of Life Sciences, Nanjing
            Agricultural University, No. 1, Weigang Road, Xuanwu District,
            Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04 September-2018
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 174.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004138485.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 18:33:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,689
            CDSs (total)                      :: 7,636
            Genes (coding)                    :: 7,552
            CDSs (with protein)               :: 7,552
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 19 of 84
            Pseudo Genes (incomplete)         :: 74 of 84
            Pseudo Genes (internal stop)      :: 2 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:27:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 39539
            Orig. end                         :: 50391
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RYEV01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5852)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    83..98
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.5"
                     /tool="antismash"
     misc_feature    complement(140..156)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.41"
                     /tool="antismash"
     misc_feature    complement(325..338)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.13"
                     /tool="antismash"
     misc_feature    complement(832..846)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: strong, score: 20.76"
                     /tool="antismash"
     gene            complement(986..1285)
                     /locus_tag="EKO19_RS38490"
     CDS             complement(986..1285)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EKO19_RS38490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157995527.1"
                     /transl_table=11
                     /translation="MSNVVDLLERMGQSSRLQSLTGEQLAQELAQAGVAPAVQAAIVQQ
                     RPRQLEELLGASHNVCCLVHAPQDEEPEEDEPEDDKRVHSSALAGADNRVARAS"
     gene            complement(1492..2034)
                     /locus_tag="EKO19_RS31420"
     CDS             complement(1492..2034)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024698.5"
                     /locus_tag="EKO19_RS31420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_129781384.1"
                     /transl_table=11
                     /translation="MHVFPVETARLRMRPIVADDAPLYEHLYTDAETMRFIGAPLAPER
                     AARSFRSALAGMQRRPIERLFLTVVEKASQTDVGICSLQDLDASERSVQAGVMFVEAAR
                     AKGYSKEGFTGLIQQVFAQLPVDELWVQFATDHLAVQRAVLSVGFARRGQAADGQGNAW
                     SVQRGSWSPPSAFWNAR"
     gene            complement(2046..2852)
                     /locus_tag="EKO19_RS31425"
     CDS             complement(2046..2852)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460251.1"
                     /locus_tag="EKO19_RS31425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_129781385.1"
                     /sec_met_domain="TP_methylase (E-value: 1.8e-23, bitscore:
                     78.0, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPAGSLVCVGIGMTLGAHLTPLARDYIQNADVVFTGLSDGIIEM
                     WIARMNSDVRSLQTLYREGKSRMDTYREMVDTMLTEVRAGKRVCAAFYGHPGVFAWPTH
                     KAIEIARSEGYCAHMEPGVSAADCMYADLGFDPGKYGCQHYEASQFMFNRRRLDTAAYL
                     ILWQVGLSGDQSLTRFSTGAAYRQVLVDVLSRDYPLDHEVILYQAATLPTFRPRIIRVP
                     LGELPAAEADMHFTLVIPPAQPLQPDHHTRERLSALDRDLGLAAQG"
     gene            complement(2870..3508)
                     /gene="maiA"
                     /locus_tag="EKO19_RS31430"
     CDS             complement(2870..3508)
                     /EC_number="5.2.1.2"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="maiA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_N_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1193: glutathione S-transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013238.1"
                     /locus_tag="EKO19_RS31430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleylacetoacetate isomerase"
                     /protein_id="WP_129781386.1"
                     /sec_met_domain="GST_N_3 (E-value: 1.8e-16, bitscore: 54.8,
                     seeds: 52, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLYNYFRSSAAFRVRIALNLKGLTCDYVSKALIKNEQRAADYLA
                     VNPQGLIPALEVDGTVLSQSLAIIEYLNDLHPQPPLLPADPLARAQVRSMALAIACDIH
                     PLNNLRVLNYLRQDLAQNDDGVNTWYRHWIGEGFRGLELQVAKHSSARRHCFGDSLSLA
                     DVCLVPQMYNARRFNCDLTPFPTLVAIATHLESLPAFDSARPEVQPDAK"
     gene            complement(3505..4188)
                     /locus_tag="EKO19_RS31435"
     CDS             complement(3505..4188)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005850676.1"
                     /locus_tag="EKO19_RS31435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="WP_129781387.1"
                     /transl_table=11
                     /translation="MSFVFQPPAPPAVAVVGRTERFPVHRIYCVGRNYAAHAREMGKDP
                     EREAPFFFTKPADAIVENGARIPFPSRTSDLHHEIELVVAIGTGGANIGISQAMSHVFG
                     YAVGLDLTRRDLQAEAKDKGRPWDTAKGFDRSAPITAITPAGDVKLDDSRIWLKVNGAL
                     RQQGAIADMIWNVAEIVAELSTLFELAPGDLIYTGTPAGVAAIKSNDALEGGVDGLETL
                     KVTLA"
     gene            complement(4216..5004)
                     /locus_tag="EKO19_RS31440"
     CDS             complement(4216..5004)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="EKO19_RS31440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_129781388.1"
                     /transl_table=11
                     /translation="MSAQSDAHSLAELQRDFQRHVMHGHDRIVDAVDGSAAVPATLRLS
                     IYSEAYRLRLTDALASTLPRLQQLLGEEQFAAVASEYIELCPSSHPSIRWFGDQLPALL
                     TKLFPDQPWLAGLAAWEWAIAAAFDGADAQAVGIEALAAVAPEQWPSLTFQFHPTVQVL
                     AMHTNAPLLFKALSADEPPPEPVALEQPQSWLIWREQLKTQYRSLSPDESAALAVMRGG
                     GSFEMLCDVLCNWHDAEAVPAQAAGLLKRWVVEQMIVGVS"
     gene            complement(5001..5852)
                     /locus_tag="EKO19_RS31445"
     CDS             complement(5001..5852)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229292.1"
                     /locus_tag="EKO19_RS31445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_129781389.1"
                     /sec_met_domain="DUF692 (E-value: 7.3e-101, bitscore:
                     331.3, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPKSSPHPSLGFGLGVRPIHYDALLGERKGSVGWLEALTENYLLP
                     GGKPLHYLERLRTEYPIVLHGVSLSIGSTDPLNETYLREVKALADRVEPAWISDHLCWT
                     GVDGVNLHDLMPLPFTEPTLNHVVSRIAAVQERLGRRLVLENVSSYVNFSRSDLTEWEF
                     LREIAERADCHLLLDVNNVYVSSVNHNFDPMTYLRGIPVERVQQFHLAGHRNMGDYLID
                     THDEPVCEAVWELFHAAVERFGPVSTMIERDDNIPELDVLLAELARARDITTSAQKSKS
                     AA"
     gene            complement(5943..6200)
                     /locus_tag="EKO19_RS31450"
     CDS             complement(5943..6200)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EKO19_RS31450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_129781668.1"
                     /transl_table=11
                     /translation="MTHGKKFSTLALATAAAVMFSTAPLTAANAADEAKVKCEGVNSCK
                     GTTACATSANSCAGKNACKGTGFLMLSKADCDAAKAKLKK"
     gene            complement(6365..6685)
                     /locus_tag="EKO19_RS31455"
     CDS             complement(6365..6685)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007892406.1"
                     /locus_tag="EKO19_RS31455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BON domain-containing protein"
                     /protein_id="WP_129781390.1"
                     /transl_table=11
                     /translation="MARQSTAALVLAAIMASTVVACSSTPTQQSTGQAIDDGVVTAKVK
                     AKLIEDPVTKAHQINVETFKGTVQLSGFVENDQARTRALQLARDTDGVKSVKDALEIRK
                     GG"
     gene            complement(6739..7167)
                     /locus_tag="EKO19_RS31460"
     CDS             complement(6739..7167)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EKO19_RS31460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_129781391.1"
                     /transl_table=11
                     /translation="MTKTNIYFMIAAAGLALAGCNKAESPAEVQHDVADARAEAQRDVT
                     DAQADAQEKMADAQKDVADAQADHDPNDVADQARDASETAAQGDFKVAVARAEATHKIA
                     VEKCEALQGNAQKDCKDRADADLDNAKRAAEARRDGAG"
     misc_feature    complement(7324..7339)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 20.34"
                     /tool="antismash"
     gene            7434..8855
                     /locus_tag="EKO19_RS31465"
     CDS             7434..8855
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF017077.5"
                     /locus_tag="EKO19_RS31465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CHASE3 domain-containing protein"
                     /protein_id="WP_129781392.1"
                     /transl_table=11
                     /translation="MGDVTHSPNASLSTPPAATGGVPLHVRIVIVISIALLVVNATLLV
                     INVGATIDSHAQYARSYEIKRSLSTFQSVITAAESGQRGYLLTGQTGYLEPYYVAMRSW
                     RSELEKLRTLTGDNAARQKDIADLENLTAAAVSRLKATISSSPQPGPNGTADVAGTERA
                     TTTMDRVRGIVDRMMAEEDARIEALRREVLRDLLVAGGLALLTTVVTVAVLIGLNKLLR
                     KYVRDRETAENAMLEANQVLNEQVEQRTSELTELSQHLIRVSEEEKAKLARELHDTLGS
                     NLTAINMDLNWVQRRLPEAAREVKERLQRALKMLSETVELKHEVIEGLRPSHLDNLGLA
                     FAMRSHCREFTRRTGIPCELDVQEDFDDLDPAWSIAFYRIAQESLTNIAKHAQASKVRV
                     ILAREDGGIRLRIIDDGVGVAEESLSKPKSHGIVGMRERMRLMGGRFQIRKPDEGTGTI
                     VEAFIPATNATALSA"
     gene            9018..9407
                     /locus_tag="EKO19_RS31470"
     CDS             9018..9407
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014950.5"
                     /locus_tag="EKO19_RS31470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_217705739.1"
                     /transl_table=11
                     /translation="MENHVERATHNEHRANYAGDDQDSGEESGVGSERNLAPAPVALRI
                     PVGTNLADVERWMIFATLQKCGGNKTRAAALLGVSLKTLYNRLNAYRAQGLAMDQGLND
                     AQDVAITTLQGGDLREAMTEVPQRC"
     gene            9461..10105
                     /locus_tag="EKO19_RS31475"
     CDS             9461..10105
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /locus_tag="EKO19_RS31475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_116807151.1"
                     /transl_table=11
                     /translation="MHTPIRVLIVDDHAILRAGVREMLAEEQDLQVVAEAGSAEDALQQ
                     LRNGLQVDVVVLDITLPGQSGIDLLKQLKRDQPELAILVLSMHPERSFAVRLMRAGANG
                     YVPKMIVPEELVKAVRAVGTGRRYITPLVAELLASECAADEEGPMHNRLSERELQVFTR
                     IARGVSPAVMANELGLSVKTVSTYRARILEKMGMRSNAEIAAYAVRNQLVE"
     gene            10118..10501
                     /locus_tag="EKO19_RS31480"
     CDS             10118..10501
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.5"
                     /locus_tag="EKO19_RS31480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_129781394.1"
                     /transl_table=11
                     /translation="MTAGAELTIYIVEDSPAVMERLVEAVGDIPNAKVVGSADAVAAAL
                     EGMRLSQPRVLILDIQLRGGSGFQVLKQLRASASRPEAVIVVTNHPSDDYRIASRECGA
                     DHFFDKASEFHKVREVLLQIDAA"
ORIGIN
        1 ggcgttggca atgtcgccat gtttctccgc ttcttcgtag agcacgcgat aagcgccgat
       61 cagcggcggc gtgaattgat ccttgacggc atggtcgacg gcacgcagcg ccatgcgctg
      121 cgcgttgact cccgaaccgc gcagctgata tgcgcgggcc atcaacgatt cccactccga
      181 gataccgcgc ggatagcgcg attgctcgac ctcgctatag tgtttctcga gcacgtcgat
      241 ggcgtcgtcg taacgctgct cgcgaaggag cagatgagcc tgcgtggtgc ggatggcgtt
      301 ggcggccagc gattcgccct gttgcaaaca ggcgttgatg gcggccggca gcgaaggatc
      361 gtccgccttg atccgcttgc tcttgcacat ggcgtcgaga cggatctgct cgccccggca
      421 cacgccgcgg ccacgcgcat tttccttgac gaccagctgg gcgtagtgaa tgcccagatc
      481 gtactcgccc accagcgtat agagatcggc ggcgacggac atgccctgca ggcgggcgtc
      541 cttgtcggtc acctcgggca atttgttcaa cagcttgttg agttgcagga acgcgccttc
      601 gtactgcgtg gacaaggcgc ggacattcgt ggcggtcgac ccggcacgga attgcaatgt
      661 cggatcgctc gcgctgtcga cgagcgactc cagcaaggga atggctttgg cgtagtcgcc
      721 ggaataagcg aagcgccagc cttgcaggta ctgcaggtgt tcgcgctgtt gcgccgacag
      781 ctcgccgagc tgcgcgtgca gttgatcgat cagccgggtg aattcgacgg gatcgacgct
      841 cttgatcgcg tccgcccgtt gcaacatggc ggcaacctct tcggcatcgg ccgccgcgag
      901 caggggctcg cccgcgaaca ggcaaagaga ggcgattccg cccaacagcc aacgccgggc
      961 ggaatccttg ctgacacgtc gttgctcagc tcgcccgggc gacacggtta tcggcacctg
     1021 ccagggccga cgagtggaca cgcttgtcat cctcgggctc gtcttcctcg ggttcctcgt
     1081 cctggggcgc gtggacgagg cagcagacat tgtggctggc gccgagcagc tcctcgagct
     1141 gacgggggcg ctgctgcacg atggctgcct gcacggccgg cgcgacgccc gcctgtgcca
     1201 gttcctgcgc caactgctcg ccggtcagcg attgcagccg cgagctctga cccattctct
     1261 ccaacagatc cacgacattg gacacgatgc gctcctcctt tccccgattg tttgttgggt
     1321 acgaccgctg ccccgtgcag caggagttgt aacaagccta ggacaatcgc cgcgggcggg
     1381 ctctcgaacg atccacagaa aaactgacgc cggcggtgac agcccgactt aacagaatta
     1441 tgcctgtttc gaagccaacg aggcggacag aaagggagag tcggcgcggt cctaccgcgc
     1501 attccaaaag gcggagggtg gcgaccacga tccgcgctga accgaccagg cattcccttg
     1561 cccatccgcg gcctggccac ggcgtgcaaa gcctacgcta agcacagccc gctgcacagc
     1621 cagatggtcg gtggcgaact gcacccataa ttcatccacc ggcaactgtg cgaacacttg
     1681 ctggatcagc ccggtgaagc cctccttgga atagcctttg gcgcgcgccg cctcgacgaa
     1741 catcacgccg gcctgcacgc tgcgctcgct ggcgtccagg tcttgcaggc tgcaaattcc
     1801 gacatccgtt tgtgacgcct tttccaccac ggtaagaaac agccgctcga tgggccggcg
     1861 ttgcataccg gcgagcgcgc ttcgaaaact tcgggcggca cgttcgggcg cgagcggcgc
     1921 accaatgaaa cgcatggtct cggcgtccgt atacagatgc tcatacaggg gcgcgtcgtc
     1981 ggcgacgatg gggcgcatgc gcagccgggc cgtctcgact ggaaatacgt gcacggtgcc
     2041 gtcgctcagc cttgagcggc cagccccaga tcgcgatcga gcgcggacag acgctcgcga
     2101 gtgtgatgat cgggttgcaa tggttgtgcc ggcggaatca ccaaggtgaa gtgcatgtcc
     2161 gcctcggccg cgggcaattc gcccagcggc acgcgaatga tcctggggcg gaaagtgggc
     2221 aacgtcgccg cctgatacag gatcacttca tggtccaggg gatagtcgcg actcaaaaca
     2281 tcgaccagca cctgtcgata cgcggcgccg gtggaaaaac gggtcaacga ctgatcgccc
     2341 gacagcccca cctgccacag aatcagatac gcggcggtat ccagtcgccg ccggttgaac
     2401 atgaactgac tggcttcgta atgctggcag ccgtacttgc cgggatcgaa gcccaggtcg
     2461 gcatacatgc aatcggccgc cgatacgccc ggctccatgt gcgcgcagta accctcgctg
     2521 cgcgcgatct cgatggcctt gtgcgtcggc caggcgaaca cgccaggatg accgtagaaa
     2581 gcggcgcata cgcgtttgcc tgcgcggact tcagtcagca tggtgtccac catctcgcgg
     2641 taggtgtcca tgcgcgactt cccttcgcga tacagcgtct gcaggctgcg cacgtcgctg
     2701 ttcatgcgtg cgatccacat ctcgatgatg ccgtcggaca gcccggtgaa caccacgtcg
     2761 gcgttctgaa tgtaatcgcg cgccagcgga gtgaggtgcg ctcccagggt catgccgatc
     2821 ccgacgcaca ccaaacttcc tgctggtttc atgagggttg ctctgaggtt tattttgcgt
     2881 cgggctggac ttccggccgg gcgctgtcga atgcgggcaa cgattccagg tgcgtggcga
     2941 tggcgaccag ggtcgggaac ggcgtgaggt cgcagttgaa acgccgcgcg ttatacatct
     3001 gcggcaccag gcacacgtcg gccagcgaca gactgtcacc gaagcaatgg cgacgcgcgc
     3061 tggaatgttt cgcgacctgc agctccagcc ctcggaaacc ctcaccgatc caatgtcgat
     3121 accaggtatt cacgccgtcg tcgttctgtg ccagatcctg tcgcaggtag ttcagcacgc
     3181 ggaggttgtt cagtggatga atgtcgcagg cgatcgcgag cgccatcgat cgcacctgcg
     3241 cgcgcgccag cggatcggct ggcagcagcg gcggctgcgg atgcagatca ttgaggtatt
     3301 caatgatggc gagcgattgt gagagcaccg tgccgtcgac ttcgagcgcc ggaatcaggc
     3361 cttgcggatt tacggccaga tagtcggccg cgcgttgctc attcttgatc agcgctttgg
     3421 aaacgtagtc gcaggtgagt cccttgagat tcagcgcgat gcgcacccgg aatgccgccg
     3481 agctgcgaaa gtagttgtag agcttcatgc cagagtcact ttgagcgttt ccaggccgtc
     3541 gacgccgcct tccagcgcgt cgttcgactt gatagccgcg acccccgcgg gagtgccggt
     3601 gtagatgaga tcgccgggcg cgagctcgaa cagcgtggac agctcggcga cgatttccgc
     3661 cacgttccag atcatgtcgg caatcgcgcc ctgctggcgc aatgcgccgt tcaccttgag
     3721 ccagatcctg ctgtcatcga gcttcacgtc gccggcgggc gtgatggcgg tgatcggcgc
     3781 cgaacgatcg aagcctttgg cggtgtccca cggacgacct ttgtccttgg cttccgcctg
     3841 caggtcacga cgcgtcagat cgaggccgac ggcgtaaccg aagacatgcg acattgcttg
     3901 cgagatgccg atgttcgcgc cgccggtgcc gatcgcgacc accagctcga tctcgtgatg
     3961 cagatcggag gtgcgcgacg ggaacgggat gcgcgcgccg ttttcgacga tggcgtcggc
     4021 gggcttggtg aagaagaacg gtgcttcacg ctccggatct ttgcccatct cccgcgcgtg
     4081 cgccgcgtag ttgcgaccca cgcaataaat gcgatgcacg ggaaagcgct cggtgcgccc
     4141 gacgacggcg acggcggggg gcgccggcgg ctggaaaacg aaggacatag ctggcagccg
     4201 aaaagtttcg ggccgctagg atactccgac gatcatttgc tccaccaccc agcgcttgag
     4261 caagccggcg gcctgcgcgg gcacagcctc ggcgtcgtgc cagttgcaca gcacgtcgca
     4321 gagcatttcg aacgagccgc cgccacgcat gactgccaat gccgcgctct cgtccggcga
     4381 cagtgagcgg tactgagtct tgagctgttc gcgccagatc agccacgact gcggctgctc
     4441 gagggcgacg ggttcgggcg gcggctcatc cgccgatagc gctttgaaca atagcggcgc
     4501 gttggtgtgc atcgccagca cttgcaccgt cggatgaaac tgaaacgtca gcgacggcca
     4561 ttgctcgggt gcgacggcgg ccagcgcctc gatgccaacg gcttgggcat ctgcgccatc
     4621 gaaagcggcg gcgatggccc attcccaggc ggccagcccg gccagccagg gttgatcggg
     4681 aaacaatttc gtcagcagtg ccggcaactg gtcgccgaac cagcgaatcg atggatggct
     4741 cgacgggcac agctcgatgt actcgctcgc caccgcggcg aactgttctt cgccgagcag
     4801 ctgttgcagg cggggcagcg tgctcgccag cgcgtcggtc aggcgcagtc gatacgcttc
     4861 ggaatagatg ctcaggcgta gtgtcgcggg cacggccgct gagccatcga ccgcatcgac
     4921 gatgcggtcg tgaccatgca tgacgtggcg ctgaaaatcc cgctgcagtt cggccagcga
     4981 gtgcgcgtcg gattgagcac tcatgcggcg gacttcgact tctgagctga cgtcgtgatg
     5041 tcgcgcgcgc gggccagctc cgccagcaac acgtccagct cgggaatgtt gtcgtcgcgc
     5101 tcgatcatgg tcgataccgg gccgaagcgc tcgacggcag cgtgaaacaa ctcccacacc
     5161 gcttcgcaga ccggttcgtc gtgcgtgtcg atgagatagt cgcccatgtt gcgatggccg
     5221 gcgagatgga actgctgaac gcgctccacc gggatgccgc gcagatacgt catcggatcg
     5281 aaattgtgat tcacgctgct gacgtagacg ttgttcacat cgagcagcag gtgacaatcg
     5341 gcgcgctcgg cgatttcgcg caggaattcc cactcggtca ggtccgagcg gctgaagttc
     5401 acgtagctcg acacgttttc cagcaccagc cggcggccga gccgttcctg aacggcggcg
     5461 atgcgcgaaa ccacgtggtt cagcgtgggc tcggtgaagg gcagcggcat caggtcgtgc
     5521 aggttcacgc cgtcgacgcc ggtccagcac agatgatcgg aaatccaggc cggctcgacg
     5581 cggtcggcca gcgctttcac ctcgcgcagg tacgtttcat tgagcgggtc ggtgctgccg
     5641 atggacagcg acacgccatg gagcacgatc gggtactcgg tgcgcagccg ttccagataa
     5701 tgcagcggct tgccgccggg gagcagatag ttctcggtca gcgcttcgag ccagccgacg
     5761 ctgcctttgc gctcgccgag cagggcgtcg tagtgaatcg ggcggacgcc gagcccgaag
     5821 ccgagcgacg gatgagggga cgatttgggc actgaacact cgtgggctga tcaggaaacg
     5881 gcgcggtgag ccgacgcaag accgcgccgc cctgacccta tcagaccggg cctggagctg
     5941 aattatttct tcagcttggc cttggcggcg tcgcagtcgg ccttgctcag catcaggaat
     6001 cccgtgccct tgcaggcatt cttgccggcg caggaattgg ccgaggtggc acaggcggtc
     6061 gtgcccttgc acgagttcac gccttcgcat ttcaccttgg cttcatccgc cgcgttggcg
     6121 gcggtcagcg gcgcggtgct gaacatgaca gcggccgccg tcgccagagc cagagtagag
     6181 aatttcttgc cgtgggtcat cgatgccatt cctcactgga aaagtgcgct tcgaaggcga
     6241 agcgctggat tatacgcagt gggccggcaa tcggattcac cggcgcggct gtcatgaacc
     6301 ggcaataaaa aggggcgggt gattgcgcac ccgcccctgt tttccgctac gcggatcggt
     6361 ggagtcaacc gcccttgcgg atctccagcg cgtccttgac gctcttcacg ccgtcggtgt
     6421 cccgggccag ttgcagcgct cgcgtccggg cctggtcgtt ctcgacgaag ccgctcagct
     6481 gcaccgtgcc cttgaacgtc tcgacgttga tctgatgcgc cttggtcacc ggatcctcga
     6541 tgagcttggc cttgaccttc gccgtgacga caccgtcgtc gattgcctgg ccggtcgatt
     6601 gctgcgtcgg ggtcgacgag caggcgacga cggtcgaggc catgattgcg gccaacacca
     6661 gtgccgccgt ggattgccgt gccatattcc ctcgctcctg aactgacttc agttctggtt
     6721 tcgttgtgac tctagctgtt aaccggcgcc atcgcgacgg gcttcagccg cgcgcttggc
     6781 gttgtccaga tccgcatcgg cgcggtcctt acagtccttc tgggcattgc cttgcagggc
     6841 ctcgcacttc tcgacagcga tcttgtgcgt ggcttcggcg cgcgctacag cgactttgaa
     6901 atcgccctgc gcagcagtct cgctggcatc acgggcctga tcggcgacat cgttcgggtc
     6961 gtgatccgcc tgtgcatcgg ccacgtcctt ctgcgcatcc gccatcttct cctgcgcatc
     7021 ggcctgcgcg tcggtcacgt cccgttgcgc ttctgcccgc gcatcggcaa cgtcgtgctg
     7081 aacttcagcg ggcgactctg ctttgttgca gcccgccagc gccaggccgg ctgcggcgat
     7141 catgaaatag atattggttt tggtcatctc actatcctcg tctgtgacct catggtctgt
     7201 tgactaagta accactcacg ggccggctga atcctcctgc ggaaggaccg gttcgttgtg
     7261 gaattcgcgt cgataacgtg ccgatgcaat gcaaggttcc tcgcacaccg ccgggccgcg
     7321 gcggtttgca attgtgagca tgggcagggc tgggtgcgtc ggccacggct gacgaaggaa
     7381 cccagaaatg ttcacagtcg ttgctggtca cagctttgag cgcacaggag ttcatgggcg
     7441 acgtcactca ttcgccgaat gcatcgttga gcacaccgcc ggccgcgacc gggggcgtgc
     7501 cgctgcacgt gcgcatcgtg atcgtgatct cgatcgcgct gctggtggtc aacgccacct
     7561 tgctggtcat caatgtcggc gcaaccatcg attcgcatgc tcaatacgcg cgctcgtacg
     7621 agatcaagcg ctcgctcagc acgtttcaat cggtgatcac ggcggcggaa tcgggccagc
     7681 gcggctattt gctgaccggt cagaccggat atctcgagcc atattacgta gcgatgcgca
     7741 gctggcgcag cgagctggag aagctgcgca ccctgaccgg cgataacgcc gcccggcaaa
     7801 aagatattgc cgacctggag aatctcaccg ccgcggcggt cagtcgcctg aaggcgacaa
     7861 tcagcagcag tccgcaacct ggcccgaacg gaacggcgga cgttgcaggt accgagcgcg
     7921 cgactaccac gatggatcgg gtgcgaggca tcgtcgatcg catgatggcc gaggaagacg
     7981 cgcgcatcga agcgttgcgt cgtgaagtgt tgcgcgactt gctggtcgcg ggcgggctgg
     8041 cgttactgac gacggtcgtg acggtcgccg tgctgattgg cctgaacaag ctgcttcgta
     8101 agtatgtgcg cgatcgcgag accgccgaga acgccatgct cgaggcgaat caggtcctga
     8161 acgagcaggt cgagcagcgc acctcggagc tgaccgagct gtcccagcac ttgatccgcg
     8221 tctccgaaga ggagaaggcc aagctcgcgc gcgagctgca tgacacgctc ggcagcaatc
     8281 tcaccgcgat caatatggac ttgaactggg tgcaacgccg cttgcccgaa gccgcgcgcg
     8341 aggtgaagga acgtctgcaa cgggcgctga agatgttgag cgagaccgtc gagctcaagc
     8401 acgaggtcat cgagggtctg cggccgagcc atctggacaa tctggggctc gccttcgcca
     8461 tgcgctcgca ttgtcgcgag ttcacccgtc gcaccggcat tccgtgcgag ttggacgtgc
     8521 aggaggactt cgacgacctg gatccggcct ggtcgatcgc gttctatcgg atcgcgcagg
     8581 agtccctgac caacattgcc aagcacgcgc aggcaagcaa ggtgcgcgtg atcctggcgc
     8641 gcgaggacgg cggcatccgt ttgcgaatca tcgacgacgg cgtcggggtc gcggaggagt
     8701 cgctgagcaa gcccaagtcg cacggcatcg tgggcatgcg ggaacgaatg cgtctgatgg
     8761 gagggcgctt ccagatccgg aagccggatg aaggcaccgg gaccatcgtc gaagccttca
     8821 ttccagccac caatgcgacg gcgctgtcgg cgtgaaggcg cgcttttgcc ccaaaggggt
     8881 gccattccgg gtggatgtca atacgtaaga aacgtctgac ggtacgaact tcactttgtt
     8941 gggaactttt ccttcccctg aggcagttgt gatccatcgg gccaatgagt gtaaagctga
     9001 ccgtcaggag cgagcgcatg gagaatcacg tcgaacgagc gacccataac gagcatcgtg
     9061 ccaactacgc gggcgatgac caggattcgg gcgaggaatc aggggtgggt agcgagcgca
     9121 acctagcgcc ggcgcctgtg gcattacgca tcccggtcgg caccaatctg gccgacgtgg
     9181 agcgctggat gatattcgcg acgctgcaga aatgcggggg aaacaagacc cgcgcagcgg
     9241 cgttgctggg ggtgagtctg aagactttgt acaaccgcct caacgcatac cgcgcgcaag
     9301 gcctggccat ggatcagggc ctgaatgacg ctcaggacgt ggccatcacc accctgcagg
     9361 ggggcgacct ccgtgaagca atgaccgagg taccacagcg atgttgaatc caaccgaatt
     9421 aggctctcgg gagaacgagg ccagggacgt gttgcaagcg atgcacacgc caatccgcgt
     9481 cttgatcgtg gacgatcacg ccatcctccg cgccggtgtg cgcgagatgc tggcggagga
     9541 gcaggatctc caggtcgttg cggaagccgg ctccgccgag gatgcgcttc aacagttgcg
     9601 caatggcttg caggtcgatg tcgtggtcct cgacatcacg ctgccgggcc agagcggcat
     9661 cgatctgttg aagcaattga aacgcgatca gccggagctg gcgatcctgg tgctgagcat
     9721 gcacccggaa cgcagcttcg cggtccgctt gatgcgggcc ggcgcgaatg gctatgtgcc
     9781 gaagatgatc gtgcctgagg agctggtgaa ggccgtgcga gcggtcggca ccgggcgccg
     9841 ctacatcacg ccgctggtgg ccgagctgtt ggcctccgag tgcgcggccg atgaagaggg
     9901 cccgatgcac aaccggctgt cggagcgcga gctgcaggtg ttcacgcgca tcgcgcgcgg
     9961 tgtttcgccg gcggtcatgg ccaacgagct gggcctgagc gtgaagaccg tgagcacgta
    10021 tcgggcgcgc attctggaga agatgggaat gcgcagtaac gccgagatcg cggcgtacgc
    10081 cgtccgcaac caactggtgg aatgagacag ttggatcatg actgccgggg ccgagctgac
    10141 gatctatatc gtcgaggatt cgccggcggt catggagcgg ctggtggaag ccgtcggtga
    10201 cattccgaat gccaaggtgg tgggcagcgc cgatgcagtc gctgccgcgc tcgaaggcat
    10261 gcggttgtcg cagccgcgcg tgctgatact cgatatccag ttgcgcggcg gcagcggctt
    10321 ccaggtgttg aagcaactac gcgccagcgc tagtcgaccc gaggctgtca tcgtagttac
    10381 gaatcatcca tccgatgatt accggattgc gagccgcgag tgcggcgccg atcatttctt
    10441 cgacaaggct tcggagtttc acaaagtcag agaggtgctg ttgcagatcg acgctgcctg
    10501 agcaaatccg taatcgccga aaatttacgc agctgaatca cggaacatac tcaggatcct
    10561 gcgcgagcgc tcgtgcgttg tgactcaact gaactgaatc gaaccctgcg caactcgtta
    10621 catggacctc cccaggacga tgggggcacc cgctgagaac ccatcggaca ccatgctgac
    10681 ggctgcgcac ttcaaaggcc gcgccccaaa aagcgcggcc ttcttttctt cagcggtcgc
    10741 gatcttcgtg gctgatattg cggctccact cggcgcggct gcgcgaggag tcgtactcga
    10801 ccgccgtctc tgaatgagtg ctgcgacatt cgtcctgcca acgcacgacg ac
//
