LOCUS       NZ_RYEV01000003        13290 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Peristeroidobacter soli strain JW-3 Scaffold2_1, whole genome
            shotgun sequence.
ACCESSION   NZ_RYEV01000003
VERSION     NZ_RYEV01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Peristeroidobacter soli
  ORGANISM  Peristeroidobacter soli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Steroidobacterales; Steroidobacteraceae; Peristeroidobacter.
REFERENCE   1  (bases 1 to 1621979)
  AUTHORS   Huang,J.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2018) College of Life Sciences, Nanjing
            Agricultural University, No. 1, Weigang Road, Xuanwu District,
            Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04 September-2018
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 174.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004138485.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 18:33:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,689
            CDSs (total)                      :: 7,636
            Genes (coding)                    :: 7,552
            CDSs (with protein)               :: 7,552
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 19 of 84
            Pseudo Genes (incomplete)         :: 74 of 84
            Pseudo Genes (internal stop)      :: 2 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:27:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1075307
            Orig. end                         :: 1088597
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RYEV01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13290
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="3"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13290
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13290
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8290](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8290
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            200..1522
                     /locus_tag="EKO19_RS13500"
     CDS             200..1522
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408779.1"
                     /locus_tag="EKO19_RS13500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_129776909.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 5e-44, bitscore:
                     147.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWRRCKQSKLLAVLLLKVSLIPAVSLAVPTAVFSQAAAPAGTTVI
                     VGASVVNVEDGSVIPNAVMLIEGDRIKAVGPASAVSVPADAQKIRMDGKWLVPGLLNMH
                     THFGLKLPGPAGAALENETDMQLVLRMADNARQSLYAGVTTVRLVGEEHGSDFALKAAI
                     DGGTAVGPRIQSAGEIIATTGGHGFLEADGTAELAKAVRQQIKNGATWIKVAISGGISD
                     SHGSIAASTMTHEEMQTVIEVAHRNGVKVTAHNGSPVAADEALALGIDCFEHAYHLTEK
                     QLRTMQAKGTWLVPTAVVTDEGAMEFFRKIGSPPWYLERVKSTREDHLKMLRAAVKLGV
                     NIALGTDQFPFEPNAGTTATVREAELYVSAGMTPLQALQAGTLKPAALLDMSKDVGSLK
                     PGHYADIIAVDANPAQNIAALRTINFVMKGGQVVRHDQPLP"
     gene            1610..2530
                     /locus_tag="EKO19_RS13505"
     CDS             1610..2530
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408771.1"
                     /locus_tag="EKO19_RS13505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_129776910.1"
                     /transl_table=11
                     /translation="MDRHSVQWRGYIPAITTPFTADGALDLDALDRLLTWLHQEGMHGI
                     IVGGTQGEWCTIDAAERKQLMQAVARKLSGKLPLIAGCSGYTAREVAAHAKLAADHGFN
                     GILVTPPPYMVPTDREILEFYRDVNDRVSLPICVYNWPPGTNVDMSRDLLQQIAGLSNV
                     VAIKNSTADLRRFVDVFFALKDEVRVFGVPMNDLGILLVQQGADGTMGAGAVLGREQPG
                     YFNAIWENDIPRAHQLGARNELLMRAWFNADYTGRFGSAQAIFKAALNELGLPGGYPRR
                     PLLPLEPEGMSVVRQTLAQLGKLGA"
     gene            complement(2552..3871)
                     /locus_tag="EKO19_RS13510"
     CDS             complement(2552..3871)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012312.5"
                     /locus_tag="EKO19_RS13510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_129776911.1"
                     /transl_table=11
                     /translation="MKKIPNFRWVIVLLLFAATAISYVDRQVLTVLAPTLRDQLQINNT
                     GYASILTAFLLAYTFMQPVMGWLIDRIGTRMGFALIMAWWSIAAGLHAVANSVASFAVC
                     RFLLGAGEAGSWSACVKAVSEWFPRKERGFATGLWSAGVSAGLVISVPIVAWINLTLGW
                     RWAFVLTGLVGLVWLAAWLWLYRLPRAHSAVTDEELRHISEDSAPDPSVSRGISSAALP
                     ESRSLLVKRTPYLQLLRSRNVWAVIAARVLADPWVWFYYFWIPEYLTRSAGFTAADIGK
                     YAWIPFAAQGVGIVLGGAFSDLLCRRGMSVLPARLTVMLIGMLLMTAGISAAFDLGITI
                     IFVGISTAMLGFGLWAPNMMSLCADAFPRDSVGSVVGLGGMGAGAGGMVFTMATGWTLD
                     TYGYAPVFIAAGAIPLLAFAVLFTLLERPKASTVNLVRTF"
     misc_feature    3856..3870
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.06"
                     /tool="antismash"
     misc_feature    complement(3858..3869)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.47"
                     /tool="antismash"
     gene            complement(3917..4828)
                     /locus_tag="EKO19_RS13515"
     CDS             complement(3917..4828)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.5"
                     /locus_tag="EKO19_RS13515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_129776912.1"
                     /transl_table=11
                     /translation="MHGEIRLRYLYEAARLGTMRAASDELNVATSSISRQIAALEKELG
                     MRLIEGGRRKIKLTEAGNAAFAYYREMQAHEEVFLSRVAELRSIRSGTINLAVGEAFIN
                     DSFSEVLQEFMRRFPGLTVRVKMSGSNDAVALVREDEAHFGLIFDVPKDPKVRARLTLS
                     QPLKAIVHPKHELAGRKSVKLGDLKDYSIGLPEDSFRIRQIVREAEHAEGVFLEPGLIA
                     NSMMLLKDFAKCGRGLTFLPEFLAQPQLTEGKLVAIPIENPVLNSTRISLITRTGRQVP
                     NGVYRLMLRVEAHLKSLIAPNS"
     gene            5001..6197
                     /locus_tag="EKO19_RS13520"
     CDS             5001..6197
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550785.1"
                     /locus_tag="EKO19_RS13520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_129776913.1"
                     /sec_met_domain="DAO (E-value: 6.3e-57, bitscore: 190.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRFDVVVLGGGLVGCAAAYYLARRGASVRLIEQGDLNREASGRN
                     AGSLHFQLEYRLIRYGDERAAQFAQIIPLSRVAIEDWRGLEAELQADLEVGMHGGLIVA
                     ESESDVALLEKRQALQEKWGLGSSLLSASEVRRIAPYLADSVIAAGYFPHEGHANPRLV
                     TPAFARRAAALDASIQTQTRVTALLRDGAEWVVSTVDRAGCTQDVMAGAVLNAAGAWAG
                     EIGALAHLHLPIFAVPLSMNVIERAAPLIPHLVQHASQRLTLKQVAAGNVLVGGGWSGR
                     FAREGSAMSTQRRAEPVIENVMANLSLAARLVPAIGGLHLLRSWAGITGITTDQMPLLG
                     AVPEAPGFFVAAGGAAFTHGPTYARLISELIVDGRPSMSIDLYSPARFSHINNFMASV"
     gene            6309..6596
                     /locus_tag="EKO19_RS13525"
     CDS             6309..6596
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810481.1"
                     /locus_tag="EKO19_RS13525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_129776914.1"
                     /sec_met_domain="Fer2_4 (E-value: 8.8e-25, bitscore: 83.0,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRRITLGVVRPAAVNIFVNGKAVEAYEGESVLTALMAGGVLVMS
                     RDSFGRMRAPFCNMGVCFDCMVEVEDGSSRVRACLTPVRAGLRVIVPDHN"
     gene            6596..8290
                     /locus_tag="EKO19_RS13530"
     CDS             6596..8290
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF019604.5"
                     /locus_tag="EKO19_RS13530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_129776915.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2e-09, bitscore:
                     33.5, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.2e-08, bitscore:
                     31.7, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVANEQQAAVATCEVAIIGAGPSGLAAAALLREHGAAVTIIDEQA
                     RAGGQILRQPPKSYSVANWLPAKLYDGVKVALRAVEERQDIDWRFQSTVLGIMRPSPYR
                     ALRAGAVRSNHIDNNTNVAGTEPDLASGSQDTRGEGSAGYGSAGVRDSRNPGGIAGSEL
                     AGVSDGRNLGSIAGSGLCGGGAHELWIHGPNGLYLLRANAVLLAPGCYERPLAFPGWTL
                     PGVMGTGAIQTFVKSQQFVPGNRFLLAGSHPLQLVVADQLLSAGAQVAAVVFTQRKQQA
                     WLLMRHPLVALRHHRQLLETSHIFRRLRRAGVPVIFGHTIVSADGAAVVERATIAAIDA
                     KGTIDRHKTKVFDCDRVGICHGFLTSSELARQAGAEMHWKDHAGGWLARHNEWLESSVK
                     NLFLAGEITGVDGADAALEKGRIAAVGILRSLGRVDDRQAHNLAATARNRLSHQQSFAA
                     ILQELARPPASLALQTMSDDTILCRCESIKRGELCQALADNKHILSADAAKLLTRVGMG
                     LCQGRLCGDNVARVIAEARGVQPGKVGPFQAQAPVKPVPLAAFMRRP"
     misc_feature    8335..8349
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.46"
                     /tool="antismash"
     misc_feature    8345..8356
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.3"
                     /tool="antismash"
     misc_feature    complement(8555..8570)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.78"
                     /tool="antismash"
     gene            8559..9839
                     /locus_tag="EKO19_RS13535"
     CDS             8559..9839
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954012.1"
                     /locus_tag="EKO19_RS13535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sugar MFS transporter"
                     /protein_id="WP_129776916.1"
                     /transl_table=11
                     /translation="MNSDSGAQPSYMPALLVLTSLFFMWGFITSLNDILIPHLKAIFTL
                     SYVQAMLIQFSFFGAYFVMSVPSGYLVEKLGYRRGIIIGLSTAGIACLCFYPAASMQSY
                     PLFLAALFVLASGITLLQVAANPYVTILGPSDTAASRLNLTQAFNSLGTTVGPFLGSMF
                     ILSAAVSAGVAVDTAKEIQTVQGPYLVLAGLLFLIAIVFSQFKLPVVKGTDATTAGADD
                     HNVPSVWKHSHLVLGAVAIFTYVGAEVAIGSLLVNFMSQPDVGGLTEKTAGSYLSLYWG
                     GAMVGRFIGAFVLRKIKPGLVLAFNAACAIALLLIAMSLSGKVAMWAVLAIGLFNSIMF
                     PTIFTLGVAKLGRHTGEGSGVLCMAIVGGAIVPVIQGYFADTIGLLSSFFVPAICYAYI
                     VFYGLKGHVVRSVPDTEISARGARANM"
     gene            9931..11724
                     /locus_tag="EKO19_RS13540"
     CDS             9931..11724
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.5"
                     /locus_tag="EKO19_RS13540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_129776917.1"
                     /transl_table=11
                     /translation="MTDGNWQRVAGRTHQRGFIDGALPMLVKRAVVAQAVLILAALWSY
                     ATWRVYSDKAETLESARHELRAVAAGMYVHLQAVLNDSLGAARTAAQAIDANGGLSEIP
                     PETAAELLARELSSGDYVRGLFVLTPNKFVAAVRNRDTQTLDTPPPWLQPAIDETRGVF
                     IGKTLEDPIESGQRAFPIAVRTVDSSGATVWAGALLGVDALDQLYETMAIPGGALSVTS
                     TAGDLLLRVPMPATAHKGQINVRDTDVYRGGADLAEEGGFVESPSPFASGTWIYAIRRL
                     GGYRLAVSASREKAAILQQWHDRTRGLTIVLAGISILFIALTLLLRHFINRLEKANTNL
                     GQLNAELESRVAARTNELQEANQQLALTNEELEAFTASASHDLRSPLGTIAGQAGLLRD
                     DLGGVMTDVITQRIDRIQSNVTRSSEIIDGLLSLARVSRQELLNERVDISTLARNVVED
                     LRQQYPRHKVECVIESNLTVDADPRLMKSLLGNLLGNAWKYTSRTPHARVEVSCTQATG
                     GIVFSVRDNGAGFDMSRSQRLFEPFQRLHSPAEFPGVGIGLAIVARIVRRYGGKITVDS
                     EPGRGTIFRFTLPAAAVTNKE"
     misc_feature    11736..11754
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.33"
                     /tool="antismash"
     gene            11772..12353
                     /locus_tag="EKO19_RS13545"
     CDS             11772..12353
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704824.1"
                     /locus_tag="EKO19_RS13545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="WP_129776918.1"
                     /transl_table=11
                     /translation="MLNPSVARLRIVGIGGTTKAGSSTEKALVACLTAAGRLGAEVQVI
                     PGAALASLPLYSPEETLRTPEQLRFVDTVRQADGLILATPAYHAGMSGLLKNAIDLLED
                     LRDEPRPYLDGRAVGCIVTAYGWQGAGTTLTSVRTIVHALRGWPTPLGVTLNTAIPLFD
                     ATGSCMDEKTQTQFEMLAAQVVGFAARFAA"
ORIGIN
        1 tgtcgcggtg ccggggagga gatcccccac cctggcgacg ccggaccacc gacgcctctg
       61 aatccgaatc cgacgacgtg gcgacaccag accacccacg cttcttaaat ccgactccga
      121 gcgaggccat gcagattgac ctgcgatagt ggccgcttcg atgatatggc tacaggcatt
      181 tcggagcggg gaaacgatca tgtggcgacg gtgtaagcaa tcgaagctgt tggctgtgct
      241 tttgctgaag gtctcgctca tccccgccgt gtcgctagcc gtacccactg cagtgttctc
      301 gcaggcagcc gcgcctgcag gcaccacagt catcgtcggt gcatcggtcg taaatgtcga
      361 agatggctcg gtgattccca acgcggtcat gctcattgaa ggcgatcgca tcaaggccgt
      421 ggggccggct tcggccgtga gtgtgccggc ggatgcgcag aagattcgta tggatggcaa
      481 gtggctcgtg cccggcctgt tgaacatgca tactcatttc ggcctgaaat taccgggccc
      541 cgcgggtgcg gcgctcgaga acgagacgga catgcagctg gtgctgcgca tggcggacaa
      601 cgcgcgacag tcgttgtatg caggcgtgac gaccgtccgt ctcgtcggtg aagagcatgg
      661 cagtgatttc gcgctgaaag ccgccatcga tggtggcacc gccgtcggtc ctcgtatcca
      721 atccgcaggt gaaatcatcg caaccaccgg cggacacggt tttctcgaag ccgatggcac
      781 ggccgagctg gcgaaggcgg tgcgccagca gatcaagaac ggcgcgacct ggatcaaggt
      841 cgcgatctcc ggcggcatct ccgattctca cggcagcatt gccgcctcga ccatgacgca
      901 tgaggagatg caaacggtca tcgaggtcgc gcatcgcaat ggcgtgaagg tgacggctca
      961 caacggctcg ccggtcgcgg cggatgaagc gctggcgctg ggtatcgatt gtttcgaaca
     1021 cgcctatcac ttgaccgaga aacaactgcg cacgatgcaa gccaagggaa cgtggctggt
     1081 gccgacggcg gtagtgactg atgaaggcgc gatggagttt tttcgtaaga tcggttcgcc
     1141 gccgtggtat ctggaacgtg tgaagagcac gcgcgaggat cacctcaaga tgcttcgggc
     1201 cgccgtaaag ctcggtgtga acatcgcgct cggcaccgat caatttccct tcgagccgaa
     1261 cgccggaacg acggcgacgg tgcgtgaggc cgagctctat gtgagcgccg gcatgacccc
     1321 gctgcaggca ttgcaggccg ggacgctcaa gcccgcggcg ctgctcgaca tgtcaaaaga
     1381 cgtgggctcg ctcaagcccg gccactacgc ggacatcatc gctgtggatg ccaaccccgc
     1441 gcaaaacatc gccgcattac ggaccatcaa cttcgtgatg aagggcggac aagtcgtgcg
     1501 tcacgaccag ccgttgccgt gaacgcaacg taatgtcgcg cggggggcta ttgaatcggc
     1561 gcggcattgt tcttagcgtg tcggccgtcg aggcttggaa ggtcaagata tggacaggca
     1621 ttcggtccag tggcggggct acattccggc gatcacgacg ccgttcaccg ccgacggagc
     1681 gctcgatctc gacgctctgg accgcttgtt gacgtggctg catcaggaag gaatgcacgg
     1741 gatcatcgtc ggtggcacgc agggtgagtg gtgcacgatc gatgcagcgg aacgcaagca
     1801 gctgatgcag gcggtcgctc gcaagctcag cggcaagctg ccgctcatcg cgggctgcag
     1861 cggttacacc gcgcgggaag tcgcggcgca cgcgaagctc gcggcggatc atggattcaa
     1921 tggcattctg gtcacgccgc caccgtacat ggtgccgacc gatcgcgaga ttctcgaatt
     1981 ctatcgcgac gtgaacgatc gggtgtcgtt gcccatctgt gtctacaact ggccgccggg
     2041 cacgaacgtg gatatgtcgc gcgacttgct gcaacagatc gccgggctgt cgaacgttgt
     2101 cgcgatcaag aactcgacgg ccgatctgcg ccgcttcgtc gatgtgttct tcgcgttgaa
     2161 ggacgaggtg cgcgtgttcg gtgtgcccat gaacgacctg ggcatcttgc tggttcaaca
     2221 gggggccgac ggtacgatgg gcgcgggtgc cgtgctcggt cgcgagcagc ccggctactt
     2281 caatgcgatt tgggagaacg atatcccgcg tgcgcatcag ctcggtgcca gaaacgaact
     2341 gctgatgcgc gcgtggttca acgctgatta cacggggcgc ttcggttcgg cgcaagccat
     2401 cttcaaggcg gcgttgaacg aactcggact gccgggcggt tatccgcggc ggccgttgtt
     2461 gccgctcgaa cccgagggca tgagcgtcgt gagacaaaca ttggctcagc tcggcaagct
     2521 cggagcctga gtttgtcagt gcgagtcatt ctcaaaaagt ccggaccaga ttcacggtac
     2581 ttgctttcgg tcgctcgagg agagtaaaaa gcacggcgaa ggccaacagg ggaatagcac
     2641 cggctgcaat aaagactggt gcgtaaccat aagtatcgag tgtccagccg gtggccatgg
     2701 taaacaccat gccaccggct ccggcgccca tcccgcccag accaaccacc gagccgaccg
     2761 agtcgcgcgg aaaagcatcg gcgcacaacg acatcatgtt cggcgcccac aggccgaagc
     2821 ccagcatcgc ggtgctgatg cctacaaaaa taatggtgat gccgagatcg aacgccgcgc
     2881 tgatgccggc cgtcatcaac aacattccaa tcagcatcac ggtgaggcgc gcgggcagca
     2941 cgctcattcc tcgacgacac aacagatcgg aaaacgcgcc gcccagcacg atgcccacgc
     3001 cctgcgcggc gaagggaatc cacgcgtact tgccgatgtc ggcggcggtg aagccggcgc
     3061 tgcgcgtgag atattcggga atccagaagt agtagaacca gacccacgga tcggcgagca
     3121 cgcgcgcggc gatcacggcc cagacgttgc gcgagcgaag caactgcaaa tagggcgtgc
     3181 gcttgaccag gaggctgcgc gactcaggta gcgcggctga ggaaatcccg cgggagacgg
     3241 acgggtcggg cgcgctgtcc tcgctgatat gacgcagctc ctcgtcggtg acggccgaat
     3301 gcgcgcgagg caggcgatag agccacagcc aggccgcgag ccagacgaga ccgacgagcc
     3361 cggtgagaac gaaggcccag cgccagccca acgtgagatt gatccaagcg acgatgggta
     3421 cggagatgac gaggcctgcg ctcacgcctg cgctccacag tcccgtcgcg aagcctcgct
     3481 ctttgcgcgg gaaccattcg gaaacggctt tgacgcaggc ggaccagctg cctgcttctc
     3541 ccgccccgag caagaaacga cacacagcga acgacgcgac actgttcgcc actgcgtgca
     3601 gtcccgctgc gatcgaccac cacgccatga tcagagcgaa tcccatgcgc gtgccgatgc
     3661 gatcgatcag ccagcccatc accggctgca tgaacgtgta cgcgagcagg aacgccgtca
     3721 ggatcgacgc ataacctgtg ttattgatct gcagctgatc gcgcaacgtc ggcgcgagca
     3781 ctgtcagcac ctggcgatcg acatagctga tcgccgtggc ggcgaacagc agcagcacga
     3841 tcacccagcg aaagttggga atctttttca cggtgtcagc gccgctcgag aggttcgagc
     3901 ggcgtgcgta ggtttatcag ctgttgggcg cgatcaagct cttcaaatgc gcctcgaccc
     3961 tcagcatcaa tcgatacacg ccgttgggca cttgccggcc ggtgcgggtg atcaggctga
     4021 tgcgcgtcga attcagcacc ggattctcga tcgggatcgc gaccagcttg ccctcggtca
     4081 attgcggctg cgccaggaac tccggcagga acgtgaggcc gcggccgcac ttcgcgaagt
     4141 ccttcagcaa catcatcgag ttcgcgatca ggccgggttc gaggaagacg ccttcggcat
     4201 gctcggcctc ccgcacgatc tgacggatgc ggaacgaatc ctccggcagg ccgatgctgt
     4261 agtccttgag atcgcccagc ttcacgctct tgcgaccggc caactcgtgc ttgggatgca
     4321 cgatggcctt gagcggctgc gacagcgtca ggcgcgcgcg caccttcggg tccttgggca
     4381 catcgaagat gagaccgaag tgagcctcgt cctcgcgcac cagcgcgacg gcatcgttgc
     4441 tgccgctcat cttcacgcgc accgtcagcc ccggaaaccg gcgcatgaac tcctgcagga
     4501 cttcactgaa cgagtcgttg atgaaggcct cgccgaccgc gaggttgatg gtgccgctgc
     4561 gaatgctgcg caactcggcg actcgcgaca ggaacacttc ttcgtgcgcc tgcatctcgc
     4621 gatagtaagc aaaggcggcg ttgccggcct cggtcagctt gatcttgcgg cgtccgcctt
     4681 cgatcaaccg catgcccagt tccttctcga gcgcggcgat ctgccggctg atggacgagg
     4741 tcgcgacatt cagctcgtcg ctggccgccc gcatcgtgcc gagccgcgcc gcctcgtaca
     4801 gatagcgcag tcgaatttcc ccgtgcatgc gttcccccga tagttgcctc taacgcaacg
     4861 aagtcgagtc tatgttgagc tcggcttcag attcaatcag cgtccggctc gcccgggcgg
     4921 gattcgggga acgcctgaag gcgcttgacc ggccgccggg gcggctgaat catgccgccc
     4981 gcaacaggct ctcaacaacg atgactcgct tcgacgtggt ggtgctcggc ggcggcctgg
     5041 tgggctgcgc ggccgcctat tacctggcca ggcgtggcgc cagcgtgcgc ttgatcgagc
     5101 agggcgatct gaaccgcgaa gcatccggcc gcaacgccgg gtcgctgcat tttcaactcg
     5161 aataccggtt gattcgttac ggcgacgagc gggctgcgca gttcgcgcag atcattccgc
     5221 tgagccgggt cgcgatcgag gattggcgtg ggctcgaagc cgagttgcag gccgacctcg
     5281 aagtcgggat gcacggtggt ttgatcgtgg ctgagtcgga gagtgatgtg gccttgctcg
     5341 agaagcggca ggcgctgcag gagaagtggg ggctggggtc ctcgttgctg tcagcgtcgg
     5401 aagtgcggcg gatcgcgccg tatctggccg acagcgtcat cgccgctggt tactttccac
     5461 atgagggtca tgcgaaccct cgactcgtca cgccggcgtt cgctcgtcgg gcggcagcgt
     5521 tggatgcgag cattcagaca cagacgcgag tgacggcgct cctgcgcgat ggcgcggagt
     5581 gggtggtctc gacggttgat cgtgccgggt gcactcagga tgtgatggct ggagcggtgc
     5641 tcaatgccgc cggtgcttgg gccggagaga tcggggcgct cgcgcatctg catctgccga
     5701 tctttgcggt gccgctgtcg atgaatgtca tcgagcgtgc cgcgcctttg attcctcatc
     5761 tcgtgcagca tgccagtcag cgactgactc tcaagcaagt cgcggcgggc aatgtgttgg
     5821 tgggcggtgg gtggtcgggg cggtttgcgc gcgagggttc ggcgatgagc actcagcggc
     5881 gcgctgagcc cgtgatcgag aatgtgatgg cgaatttgtc gctggccgcg cggctggtgc
     5941 ccgcgattgg cgggttgcat ttgttacgtt cgtgggctgg gatcaccggg attacgaccg
     6001 atcaaatgcc gctgctcggc gcggtgccgg aggcgcctgg gttcttcgtc gctgcgggtg
     6061 gcgcggcgtt tacgcatggg ccgacgtatg cgaggttgat cagtgagctc atcgtcgatg
     6121 ggcggccgtc catgagtatc gatctctata gcccggctcg gttcagccac atcaataact
     6181 tcatggcttc ggtgtgacat gagagtcacg cattttccga gcttcactgg tccgtgtgcg
     6241 ccatgcccag cgcttgcccg cgacgctcgt gtgaatgcgt ggcggctcgt gttgcgggcg
     6301 gtcagagcat gagcaggaga attacgctgg gtgtcgtgag gccggcggcg gtcaacattt
     6361 tcgtcaatgg caaggcggtc gaggcttacg aaggggagtc ggtgctgacg gcgctcatgg
     6421 cgggcggtgt gcttgtgatg tcgcgcgata gtttcggtcg gatgcgcgcg cctttctgca
     6481 acatgggtgt ttgttttgac tgcatggtcg aagtggaaga tggttcctct cgtgtgcgcg
     6541 cgtgtttgac gccggtgcgt gccgggttgc gtgtgatcgt gccggaccat aattgatggt
     6601 tgctaacgaa cagcaagcgg ccgttgcgac gtgcgaggtc gccatcatcg gcgccggccc
     6661 gtccggcctc gctgccgccg cgctgcttcg agagcatggt gccgctgtga cgatcatcga
     6721 tgagcaggcg cgcgctggcg ggcagattct gcgccagccg ccgaagagtt attccgtcgc
     6781 gaattggttg ccggccaagt tgtacgacgg cgtgaaggtc gcgctgaggg cggtagaaga
     6841 gcgacaggat atcgattggc ggtttcagtc gaccgtgctc gggatcatgc ggccgtcgcc
     6901 gtatcgggcg ctgcgcgccg gcgcggttcg tagcaaccac attgataaca atacgaacgt
     6961 cgctggtacg gaaccggatc tggcgagcgg cagccaagat actaggggtg agggcagcgc
     7021 tggctatgga tcggccggag tgagagatag ccgtaatcct ggtggcatcg ccggctcgga
     7081 attggccgga gtgagtgacg gtcgcaatct tgggagcatt gccggctcgg ggctctgtgg
     7141 cggtggcgcg cacgagctgt ggattcatgg gccaaacggt ttgtatttgc tccgggcgaa
     7201 tgcggtgctt ctggcgccgg gttgttacga acgtccgttg gcctttccgg gctggacgtt
     7261 gccgggcgtc atggggacgg gtgccattca aacgtttgtg aagagtcagc agttcgtgcc
     7321 gggcaatcgt ttcctgctcg cgggcagtca tccactgcaa ctcgtcgtcg cggatcaatt
     7381 gctgagcgcc ggcgcgcaag tcgcggctgt cgtcttcacc cagcgcaagc aacaagcctg
     7441 gctcctcatg cgccacccgc tcgtggcgct ccggcatcat cggcagctgc tcgaaacctc
     7501 tcacatcttc agaagactgc gtcgggccgg cgtgccggtg atctttggtc acaccatcgt
     7561 cagtgccgac ggcgcagccg tcgtagagcg cgcgacgata gcggccatcg atgcgaaagg
     7621 aacgattgat cggcataaga ccaaggtctt cgactgcgac cgcgtcggca tctgtcacgg
     7681 ctttctcacg tcatccgagc tcgcgcgaca ggccggtgcc gagatgcatt ggaaggatca
     7741 cgccggcggt tggctcgcgc gtcacaacga gtggctggaa tcgagtgtca aaaatctgtt
     7801 cctggccggc gagatcaccg gcgtcgatgg cgccgacgcg gcgttagaga agggccgcat
     7861 cgctgcggtt ggcatcctgc gttccctcgg tcgcgtggac gatcgccagg cacacaacct
     7921 ggcggcgaca gcgaggaatc gtttgtcaca tcagcaaagc ttcgccgcga tcctgcagga
     7981 acttgcccgc ccacccgcga gcctcgcgtt acaaacaatg agcgacgaca ccatcctgtg
     8041 tcgctgcgag tccatcaaac ggggcgagct ttgccaggca ctggcagaca acaaacatat
     8101 cctctccgcg gacgccgcga agctactgac cagagtgggc atgggcctgt gccagggccg
     8161 actgtgcggc gacaatgtcg ctcgagtcat cgccgaagcc cgcggtgtgc agcccggtaa
     8221 agtgggtccg ttccaggcac aagcgccagt gaagcccgta cctctcgcgg cattcatgcg
     8281 tcgaccctga ccccaagctt cgttgaggac gcacgcgacg gctcgaccat gctcgccgca
     8341 atctgaaaac gctgtctcgc cgcttctcga cgactgagcc gccccgtcgt cgtctcattt
     8401 ctccgtgcat caatggtttg cgactgatga agcgcctcgt ggcgatgagt tagcgatatc
     8461 aacgccgtga ccccctttcc gcccggcccc tctctctggc atgctgacaa cgttgtcacc
     8521 ttgagcgaca gcgtcggcag caatccgggg gatagagaat gaattccgat agcggcgcac
     8581 agccgagcta catgcctgca ctgctggtgc tgacctccct gttcttcatg tgggggttca
     8641 tcacttcgct gaacgacatt ctgattccgc acctgaaggc cattttcacg ttgagttacg
     8701 tgcaggccat gctgatccag ttcagcttct tcggtgctta cttcgtgatg tcggtgccct
     8761 cggggtatct ggtggagaag ctggggtacc ggcgcggcat catcatcggg ctgtcgacgg
     8821 cgggcatcgc ctgtctctgc ttctacccgg cggcgtcgat gcagtcgtat ccgctgttcc
     8881 tggcggcgtt gtttgtactg gcgtcgggca tcacgctgct ccaggtcgcc gcgaatcctt
     8941 acgtcaccat tctcgggccg agcgacacgg ccgcgagtcg attgaatctg acccaggcat
     9001 tcaattccct gggcaccacg gtcggcccat tcctcgggtc gatgttcatc ctgtccgcgg
     9061 cggtgagtgc gggcgtggcg gtggatacgg cgaaggaaat ccagacggtg caagggccgt
     9121 atctggtgct ggccggcctg ttgttcctga tcgccatcgt gttctcgcag ttcaaattgc
     9181 cagtagtgaa aggcacggac gcgaccaccg cgggggccga cgatcacaac gtgcccagcg
     9241 tgtggaaaca ctcgcacctg gtgctgggtg ctgtcgccat cttcacttac gtcggcgcgg
     9301 aagttgcgat cggcagcttg ctggtgaatt tcatgtccca gcccgacgtg ggtggactca
     9361 ccgagaagac cgcgggcagt tatctatcgc tgtactgggg cggcgccatg gttggccggt
     9421 tcatcggtgc gttcgtgctg cggaagatca agccgggcct ggtgctcgcg ttcaacgccg
     9481 cttgcgccat cgcgctgttg ctgatcgcga tgagcctgtc gggcaaggtt gcgatgtggg
     9541 cggtgctggc catcggcctg ttcaactcca tcatgtttcc gaccatcttc acgctcggcg
     9601 tggcgaagct gggccgtcac accggcgagg gttcgggcgt gctgtgcatg gccatcgtcg
     9661 gcggtgccat cgtgccggtg attcagggct acttcgcgga caccatcggc ttgctctcgt
     9721 cgttcttcgt gcccgcgatc tgctatgcct acatcgtgtt ctacggcttg aaggggcacg
     9781 tggtccgcag cgttcctgac acggagattt cggcacgagg ggcgcgcgcc aatatgtgac
     9841 gccgctccgg taaccgctgg ccccgggtgg cgggagccag tgtccgcgtg agagactgcc
     9901 ggcgcgaaga aaatcccgca cgggcaactc atgacggatg gcaactggca gcgcgtagca
     9961 ggacgcacgc atcagcgagg attcatcgac ggcgcactac ctatgctggt gaagcgtgcg
    10021 gtggtcgcgc aagctgttct cattcttgcg gcgctgtgga gctacgccac ctggcgggtc
    10081 tacagcgaca aggccgaaac cctcgaatcc gcccgccacg agctgcgagc cgtcgcggcg
    10141 ggcatgtatg ttcatttgca ggcggtcctg aacgacagtc tcggcgcggc gcgtaccgcg
    10201 gctcaggcca tcgatgccaa tggcgggttg agcgaaatcc cgccggagac cgccgccgaa
    10261 ttgctggctc gggagttgag cagcggcgac tacgttcgtg gtctgttcgt gctcacgccg
    10321 aacaagttcg tggctgccgt ccgcaatcgc gacacgcaga cgctcgatac gccgccgccc
    10381 tggttgcagc ccgcgatcga cgagacgcgc ggcgtgttca tcggcaagac gctcgaagat
    10441 cccatcgaat ccggccagcg cgcatttccc atcgcggttc gtaccgtcga cagctcgggt
    10501 gccacggtgt gggcaggtgc tctgctcggc gtcgatgcgc tcgatcagct gtacgagacg
    10561 atggcgatcc cgggcggcgc cttgagtgtg acgtccactg ccggcgatct gttgttacga
    10621 gtgccgatgc cggcgacggc tcacaaaggt cagatcaacg ttcgcgacac cgacgtctat
    10681 cgcggcggtg ccgacctggc ggaggagggc gggttcgtcg agtcgcccag tccgtttgcg
    10741 agcggcacct ggatttatgc gattcgccgt ctcggcggtt atcggctcgc ggtctcggcg
    10801 agccgggaga aagcagccat tctgcagcag tggcacgatc gcacgcgcgg cttgaccatc
    10861 gtgctggccg gcatttccat tttgttcatc gcgctgactc tgttgctccg gcatttcatc
    10921 aaccggctgg agaaggcgaa taccaatctc ggccagctca atgccgagct ggaaagccgc
    10981 gtcgccgcgc gtaccaacga gctgcaggaa gcgaaccagc aactggcgct gaccaacgaa
    11041 gagctggaag cgttcaccgc ctcggcgtcg cacgatctgc gctcgccgct gggaacgatt
    11101 gccggtcagg ccggcctgct ccgagacgat ctgggcggag tgatgaccga tgtgatcacc
    11161 caacgtatcg atcggattca atcgaacgtc acgcgctcct ccgaaatcat cgatggcttg
    11221 ttgtcgctgg cgcgtgtctc gcgtcaggaa ctgttgaacg agcgcgtcga tatttccacg
    11281 ctggctcgta acgtggtcga ggatctgcgt cagcagtatc cgaggcacaa ggtcgaatgc
    11341 gtgatcgagt cgaacctcac cgtcgacgcc gatccgcggc tgatgaagag tctgctgggc
    11401 aacttgctgg gcaacgcctg gaaatacacc agtcgcacac cgcatgcgcg cgtcgaagtg
    11461 agctgcacgc aagcgaccgg cggcatcgtg ttctcggtgc gtgacaacgg cgcgggcttc
    11521 gatatgtcac gttcgcaacg attgttcgag ccgtttcagc gcctgcatag ccccgctgaa
    11581 ttcccgggcg tcggcatcgg tctcgccatc gtcgcgcgca tcgtgcgtcg gtatggcggc
    11641 aagatcaccg tcgatagcga gcccggtcgc ggcacgatct tccgcttcac cctgccggcc
    11701 gcggccgtga ccaacaaaga gtgagagtcg ggtgataagg tgaggctccc gttcatggag
    11761 cccgcctctc gatgctgaat ccttctgttg cgcgtctgcg tatcgtcggt atcggcggta
    11821 ccaccaaagc cggctcgtcg accgagaagg cgctggtcgc ctgtctcacg gcggccgggc
    11881 ggttgggagc ggaggtgcag gtgattcccg gcgcagcgct ggccagtctg ccgctgtatt
    11941 ccccggaaga gacgctgcgg acgccggagc aactgcgctt cgtcgatacg gtccggcagg
    12001 cggacggtct gatcctcgcg accccggcgt atcacgccgg tatgtcgggg ctgctgaaga
    12061 acgccatcga cctgctcgaa gacctgcgcg acgaaccccg gccgtacctg gatggccggg
    12121 cggtgggctg catcgtgacg gcgtatggct ggcagggggc cgggacgacg ctgacttcgg
    12181 tgcggaccat cgtgcatgcc ctgcgcggct ggccgacccc gctcggggtg acgctgaaca
    12241 ccgcgattcc gctgttcgat gcgacgggct cgtgcatgga cgagaagacc cagacccagt
    12301 tcgagatgct ggcggcgcaa gtcgtcggct tcgcggcgcg tttcgccgca taaatttcct
    12361 gacagcctgc cagggacgat taaaggttta tcagttagtt cggggcctcc gggccccggg
    12421 ttactctata aacctcgcta acggacagtg aattcaatgt catatcgacg ttcccgcctt
    12481 cccgccccgt cgcagtgggt cgtgtgcgcg atgcttttgc tcgccgccgc ggggtgctcg
    12541 cgccaaccgg ccgggcaggg caagcccgtc ggcccgcaga ccatcgatgt ggagaccgcc
    12601 gcagcgacca ttcagccgct cggggtcgag atcgaagcgg tgggtacggc tcgcgccaac
    12661 gagtcggtcg aagtgacctc caaagcgtcc aacacggtca ccgcgattcg tttcgaggag
    12721 ggcgaccggg ttcgcaaggg cgcggtcctg gtcgagctca atggcgacga agcccgcgcc
    12781 gcgctggcgg aagccgaagc cgcgctcgcc gaaagtcaga acaacttcaa gcgcagccgc
    12841 gatctgtatt cgcagcaggc gctgtcggtg tcgcagctcg atctgatcga atcgacgttg
    12901 aaaggcaatc aggcgcgggt cgatgtggcc aaggcgcgcc tggcggacac catcattcgc
    12961 gccagcttcg atggcaagac cggctttcgc cgcgtcagtc cgggcagcct ggtcagcccg
    13021 ggcacggtga tcaccacgct cgacgacacg tcggtcatca agctcgattt caccgtcgcc
    13081 gaaacttatt tgtatgcatt ggagaagaat ctgccggtga ccgcggcgac cgcgggcctg
    13141 ccggggcgtg agttcaaggg caaggtcagt cagatcgatt cgcgtgtcga tccggtgagt
    13201 cgttcgatcg ccgtgcgcgc cgaattgccg aatcccaaag gcgagctgcg gccgggcatg
    13261 ttcatgacgg tgaagttgca aggcgaagtg
//
