LOCUS       NZ_CP033336            11846 bp    DNA     circular CON 03-APR-2024
DEFINITION  Streptococcus pyogenes strain TSPY165 chromosome, complete genome.
ACCESSION   NZ_CP033336
VERSION     NZ_CP033336.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1812036)
  AUTHORS   Macias,O.R., Shah,B.J., Hanson,B., Sanson,M.A. and Flores,A.R.
  TITLE     Complete genome sequences of group A Streptococcus emm types
            frequently associated with antibiotic resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1812036)
  AUTHORS   Macias,O.R., Shah,B.J., Hanson,B., Sanson,M.A. and Flores,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2018) Pediatrics, UTHSC/McGovern Medical School,
            6431 Fannin St., MSB 3.130, Houston, TX 77030, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2018
            Assembly Method :: Unicycler v. 0.4.6; Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 263.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004124135.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:58:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,798
            CDSs (total)                      :: 1,709
            Genes (coding)                    :: 1,656
            CDSs (with protein)               :: 1,656
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 31 of 53
            Pseudo Genes (incomplete)         :: 23 of 53
            Pseudo Genes (internal stop)      :: 18 of 53
            Pseudo Genes (multiple problems)  :: 17 of 53
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:19:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1397804
            Orig. end                         :: 1409650
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033336.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Anthony R. Flores, MD, MPH,
            PhD.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11846
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11846
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11846
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6846](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6846)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            189..431
                     /locus_tag="EB817_RS09610"
                     /old_locus_tag="EB817_07170"
     CDS             189..431
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="EB817_RS09610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07170"
                     /product="hypothetical protein"
                     /protein_id="WP_002985713.1"
                     /transl_table=11
                     /translation="MKQQGHTVMMCLNKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLH
                     LLPKVVPVLIHLVDKANVSWLTVILQLPILLKTIW"
     gene            953..1222
                     /gene="stcA"
                     /locus_tag="EB817_RS07165"
                     /old_locus_tag="EB817_07175"
     CDS             953..1222
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="EB817_RS07165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07175"
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_020833327.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPILKHTIERKRTAMTIGHSK
                     QNLHKKEALKHKKNKNKALFLPSGDSAIKSVINMTVRLLLDDQS"
     gene            complement(1571..1969)
                     /locus_tag="EB817_RS07170"
                     /old_locus_tag="EB817_07180"
     CDS             complement(1571..1969)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /locus_tag="EB817_RS07170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07180"
                     /product="hypothetical protein"
                     /protein_id="WP_011054254.1"
                     /transl_table=11
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLED
                     DAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTGIT
                     YGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(2062..3132)
                     /locus_tag="EB817_RS07175"
                     /old_locus_tag="EB817_07185"
     CDS             complement(2062..3132)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="EB817_RS07175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07185"
                     /product="hypothetical protein"
                     /protein_id="WP_129304879.1"
                     /transl_table=11
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGVG
                     YKEETTSQFSSDQPSQVVLYRGEANTVRFTYTDQMSLMKDIRIALDGSGKSLTAQTVPG
                     MGHVYEGLQTSARGIFTMSGVPESTVPVANPNAQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGAFPDNIKVSIN
                     PKGGLATITYGAGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQSLNEH
                     EAMVNVEPLSHVVKDNIKVIDEKSTGRFELFRPNEDEKEKPASDVKVRPAEVGSWLEPA
                     TALPSVEMSAEDRLKS"
     gene            complement(3383..3784)
                     /locus_tag="EB817_RS07180"
                     /old_locus_tag="EB817_07190"
     CDS             complement(3383..3784)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /locus_tag="EB817_RS07180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07190"
                     /product="hypothetical protein"
                     /protein_id="WP_053308564.1"
                     /transl_table=11
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLAWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVSIYFYLHL
                     IVVCYTIGMILSLGRLISPNHSIFNKLKK"
     gene            complement(3978..4258)
                     /locus_tag="EB817_RS07185"
                     /old_locus_tag="EB817_07195"
                     /pseudo=""
     CDS             complement(3978..4258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528350.1"
                     /locus_tag="EB817_RS07185"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07195"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKILDIIFNLSDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRHP
                     YPIWAAVLGGISLIVIFIYFILIVYLYLRYRYLVWKKYQKLRLILLSY"
     gene            complement(4308..4472)
                     /locus_tag="EB817_RS09615"
                     /old_locus_tag="EB817_07200"
     CDS             complement(4308..4472)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073350.1"
                     /locus_tag="EB817_RS09615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07200"
                     /product="bacteriocin"
                     /protein_id="WP_322555861.1"
                     /transl_table=11
                     /translation="MAIINGGGKDGANIFLTGMAGAAQGVTVCAQTGVFIPWQGYLLCG
                     AAANIIWPH"
     gene            complement(4788..4988)
                     /locus_tag="EB817_RS07200"
                     /old_locus_tag="EB817_07210"
     CDS             complement(4788..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="EB817_RS07200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07210"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_003060803.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LTGGCVVGFTVGGAYLGYTAN"
     gene            complement(5001..5228)
                     /locus_tag="EB817_RS07205"
                     /old_locus_tag="EB817_07215"
     CDS             complement(5001..5228)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="EB817_RS07205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07215"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_032460066.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 8.1e-16,
                     bitscore: 54.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAIS
                     FPVCGVACGYIGAKTAVTLWAGVTGATGGF"
     gene            5597..6232
                     /locus_tag="EB817_RS07210"
                     /old_locus_tag="EB817_07220"
                     /pseudo=""
     CDS             5597..6232
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /locus_tag="EB817_RS07210"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07220"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDEFFRSSLEIAMNDLLQVELSAFLGYEPYEKEGYNTGNSRNGTY
                     SRQFETKYGLVNLIIPRDRNGEFSPVLLPSYARRDDHLEEMVIKLYQTGVTTREISDII
                     ERMYGHHLFLSGSLVTKRL"
     gene            complement(6452..6634)
                     /locus_tag="EB817_RS07215"
                     /old_locus_tag="EB817_07225"
     CDS             complement(6452..6634)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /locus_tag="EB817_RS07215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07225"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_129304880.1"
                     /transl_table=11
                     /translation="MDNFLELQFKELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITGG
                     AAAFVCVASGIAAYQ"
     gene            complement(6649..6846)
                     /locus_tag="EB817_RS07220"
                     /old_locus_tag="EB817_07230"
     CDS             complement(6649..6846)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /locus_tag="EB817_RS07220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07230"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_053308565.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 9.9e-14,
                     bitscore: 48.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGAFAGA
                     HVGAIAGGLTCVGGMLFNGK"
     misc_feature    complement(6803..6817)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.12"
                     /tool="antismash"
     gene            7245..7499
                     /locus_tag="EB817_RS09515"
                     /old_locus_tag="EB817_07235"
     CDS             7245..7499
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="EB817_RS09515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07235"
                     /product="hypothetical protein"
                     /protein_id="WP_080370154.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSEQVYDLLNEIRMRYPHAHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHESLRSIRDQIQLYLDELYNSNNG"
     gene            complement(7805..8281)
                     /locus_tag="EB817_RS07230"
                     /old_locus_tag="EB817_07240"
     CDS             complement(7805..8281)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="EB817_RS07230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07240"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002985741.1"
                     /transl_table=11
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKTW
                     AIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFIYE
                     LTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     misc_feature    8238..8254
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.42"
                     /tool="antismash"
     gene            complement(8301..9197)
                     /gene="era"
                     /locus_tag="EB817_RS07235"
                     /old_locus_tag="EB817_07245"
     CDS             complement(8301..9197)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="EB817_RS07235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07245"
                     /product="GTPase Era"
                     /protein_id="WP_002985743.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     misc_feature    9250..9265
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            complement(9317..9724)
                     /locus_tag="EB817_RS07240"
                     /old_locus_tag="EB817_07250"
     CDS             complement(9317..9724)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="EB817_RS07240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07250"
                     /product="diacylglycerol kinase"
                     /protein_id="WP_002985746.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            complement(9705..10202)
                     /gene="ybeY"
                     /locus_tag="EB817_RS07245"
                     /old_locus_tag="EB817_07255"
     CDS             complement(9705..10202)
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="EB817_RS07245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07255"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_002985748.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     misc_feature    complement(10216..10230)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            complement(10361..10936)
                     /locus_tag="EB817_RS07250"
                     /old_locus_tag="EB817_07260"
     CDS             complement(10361..10936)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="EB817_RS07250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB817_07260"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_009880369.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
     misc_feature    11219..11232
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
ORIGIN
        1 acattgatca tggatatcct gattatcgtt ggtggataac ctttcgctta aaaacaatcc
       61 acttttggga aatccttata tttagtttgt aacaggaaaa ctaataaata ttaaaaagag
      121 tgttagcgac ctagcggcag ctttactctt aagtgcgttt gctgccattg acagcaaggt
      181 caaagctgat gaaacagcag gggcataccg ttatgatgtg tctgaacaaa acacttacta
      241 tcaaactatc agctccatta accagcaacg acctggtgcc actctcgacg aatgaggtga
      301 ttattatgga acccttactt catttacttc ccaaagtcgt accagtactt atacacttgg
      361 tagacaaagc caacgtatca tggttaacag tgatattaca gttacctata ctcttaaaga
      421 caatttggta gcccttcata aggaatataa aacctttgtc aaaaaaatat tacagataag
      481 ccgatcacca gtgactacga caaggctaag gttatttata accatttaat caacgaatac
      541 acttacgcca caaaataact tagcaccaat tgcaaaaccg taagcagcat caacaatcat
      601 actcctaaag ctctttatcg tggcaagtgt ggtgtttgtc aagcttatgc cgtcatgttt
      661 aaagatatgg cagaagctgc aggcttatca gcttggtatg tgataaaaaa ggattgaatg
      721 agcaccatgc ttgaaacatc gttaccattc atggtgctaa ttcctacgtt gatgctacct
      781 ggaatgattc agcatacacc acaaggtatt tccttgctgg aaaagacgtg aggaatactg
      841 agtaccgttt agatagccag tacaaccact tggctaatgc tattttaaat accagctacc
      901 aaaaaggcta aactaacaac aactacattt ttcaactaga aaggcctaaa caatgaccaa
      961 aaaacagttt tatcttatct tagctattag catactgtgt tttattcttg ccgtcagccc
     1021 taaaccacca attcttaaac acactattga aaggaaaagg actgcgatga ccataggcca
     1081 ctcaaaacaa aaccttcaca aaaaagaagc ccttaaacac aaaaaaaaca aaaataaggc
     1141 tctttttctt ccaagtgggg acagcgctat caaatctgtt attaacatga cagttagact
     1201 cctgttagat gaccaatctt agctctccta aaatcagtgg acactaccgt ttcggtagtg
     1261 ttctttttta agctaatctc atcaccacgc tttcttcaac ctaaaaaaac tgatcatgaa
     1321 agagcaaccc catcacccta acagcccact ttgcccgcac tttcttaaaa agttaggaga
     1381 aaacgatata ccaattattt tctgatccca gctccctatc attaacaaga tgtgtagcac
     1441 ggacctacat acaggtaagc tagagtcatt ctcgctattt cggacaaaaa gaggccggta
     1501 gttccctgac ctcttcttta tttatcttgt aacactcctc ttcttagcga gacttactag
     1561 cacataattc ttaagcactc ttatcattta gactaggaat aacaccatct gaatgaagca
     1621 aatgatgcgt cagatctgcc agagtatcat ctccataagt aatccctgtt tggtgctgtt
     1681 tctgatgaaa atcagatgac tccttaactt tagcagtcac ctgctggtca cttggatgaa
     1741 aattaatttc tctcttggct tcagccgcaa ctggctcgag gaacaactta ttgacattac
     1801 ggtcttcctc caaatgatta aggatagcag catcatcttc taacgaaaga accttcttgg
     1861 gtaacaaaaa ggggttattt agccttggct cagtaacgac ttccttggct actcgtgata
     1921 gtttattatt gtcttcctca ggtatcaaac ggttaggcaa tcgatgcaat tcttccggct
     1981 gtgaggcgct cacagctact tcagaagaaa cctccaagcc ctttcctgtt tgcttgtcta
     2041 aagcaaccgg caaattatta actaactttt taacctgtcc tcagcgctca tttcaacact
     2101 aggaagagct gtcgctggtt ctagccagct accaacttct gctggtctta ccttaacatc
     2161 gctggcaggc ttctccttct catcttcatt aggtctaaaa agctcaaacc gccctgttga
     2221 tttttcatct atgaccttta tattgtcttt tactacatga gacagtggtt cgacatttac
     2281 catagcttca tgctcgttaa ggctctggtc tcgtttgaat ttaccaggaa taccaggagt
     2341 cggtgttccg acagattgat cggcttcagt aggctctttt acggagcctt ttttccataa
     2401 aactgctgga ggaataatat ccgtagagcc agccccataa gtaatcgtgg ccagcccccc
     2461 ctttggatta atagatactt taatattgtc tgggaaagcc ccactacctc ctgtggttaa
     2521 aagggcaccc gcatttttaa tgagagttgt cattcgttca ataccagcaa cgccaatatg
     2581 gttcaagtca tctactggta actgatcaac agatttcatg gtgtatttcc aagcttgcgg
     2641 ttgaacaaga aaaacagttc ctttatacat tgtgttatgc atatcatcaa tgactttgaa
     2701 ataccttgta tatttggttt gtgcattagg gttagcaacg ggaacagtgc tttcaggaac
     2761 tcctgacatc gtaaaaatcc ctctagcaga agtttgaagg ccctcataaa catgacccat
     2821 accaggaaca gtctgagcgg tcaaagactt accagaacca tccaaagcaa tgcgaatatc
     2881 tttcatcaga gacatctgat cggtataggt aaacctaacc gtgttagcct cacctcgata
     2941 caaaaccact tgggagggct gatccgaaga aaactgagaa gttgtttctt ccttatagcc
     3001 aactccaaaa cgtctatcag acgaatggac tgaatctgcc gcaactgtac tactcaaact
     3061 gactagtaaa gtagccgcac aaccaactaa taaactgctc gttttaactt taacatctct
     3121 taacatcatc atgttccttt cattattttt tatttaggaa ttcgtcatac tatattttct
     3181 catattagtc taacaaaaaa ctcgtaattc tatataaatc atataaaatg acgagctttg
     3241 gcaaagatga gtatagcagg atggaaaaca gattgtttct gaaacttcaa aggtatcttc
     3301 ttgtcacgaa ctattcttta attgaatcgg ttacactaaa ctagtcccat tatttaaaaa
     3361 caaactttaa atatgattca tctcactttt taagtttatt aaatatagag tgattaggag
     3421 aaataagacg acctaatgat aaaatcattc ctattgtata gcaaacaaca ataaggtgta
     3481 aataaaagta aattgatacc tctgatatac tcgtcaaaca tcctattatt ataataaaag
     3541 caattaaagt aggaataaga attgtacact taataaataa ccaaaattgc ttaacaactt
     3601 gataccatgc gagcacttct gtcgtttgtc tttgtttgta gacaaaaacg aataaatcaa
     3661 ggataaaact ccaacagata agttgtatat ttttcattaa tggtaaatcg tagaaactac
     3721 ctatactaaa tagaatcact gcagaaataa taacaatacc taaaaatctt aatatattac
     3781 gcatatcctc acctcctcta atttatcggt agcaaatata actgaacaaa ttgttattcc
     3841 tgttatcaga gacagttata acttttttaa ccaattttca atacgcattt tgcttttaac
     3901 tctttagtct ctaaagttaa atatcataag tactgatatt gtataagtca tccattttcc
     3961 ccttaaatgt gattgcatta tagctcagta aaattaatct taatttctgg tattttttcc
     4021 ataccaagta tctataacgc aaatatagat aaacaataag tataaaataa ataaagatca
     4081 caataaggga tatacctcct agtacagcag cccaaatagg ataagggtgc ctaaagttgt
     4141 aggccacact caagatgata agggtaaaag ggacaatgta acctcccagc ttttcaacat
     4201 tttctagaaa tatcatcttt ctatcagata ggttaaaaat aatatctaaa attttcatac
     4261 cactcctcta ctacttatac atcagtgata gatagtcatt tatatattta gtgaggccag
     4321 ataatgtttg cagctgctcc acacaaaaga taaccttgcc aagggataaa tacgcctgtc
     4381 tgagcacaaa ctgtcactcc ctgcgcagca cctgccatcc ctgtcaaaaa gatatttgct
     4441 ccgtcttttc cgcctccatt tatgatagcc agaccatttt tttagcactt ccatattttt
     4501 gaagtctttt tcattttaat ttcactcttt tgtattttaa aaagctaaga gtgatatcta
     4561 aattcattgg cataatcact attttctttt atcgataaaa acaaataatg acgctaccaa
     4621 agctacgata gataacatgt ttaccgattt ttccgaaagg gataattgat tgaaaattaa
     4681 aaacactaaa tatggtatag atttatcaac atttttttat gaatggcatc aacaatgatt
     4741 aaaaacatta tagatgcatt cataataata aaaatcgtat agatcattta atttgctgta
     4801 tatcccaaat atgctccacc aactgtaaat cctacaacac atccacctgt caatactgga
     4861 tgtggcagtt tacaaaattt caatccagaa gctaagcctc caagaccacc aattacagca
     4921 tctcctggag cgtttcctcc acttatacta gccatgtcat taattgttaa ttccttcatt
     4981 gttgtcatcc tttctttcct ctagaatccg cctgtagctc ctgttacacc agcccaaaga
     5041 gtaacagctg ttttagctcc aatataacca caggctacgc cgcaaactgg aaaacttata
     5101 gcagctccag cactacctgc agcgataacc ccactgacat ttgcctgcca attattctta
     5161 ccaccctcaa ccttggcaag cttttctata gtcatttggt gaaatgtttc cagtttcttt
     5221 atctccatat cggataactt cttttcataa ttcttaaatt tgtcttgttg caacctcgac
     5281 tagtacagga tatcataaag aacagtatca tggcttcaaa atcttaaaag gccgtttttt
     5341 catcatgaaa tgtcttttta tcctctctac acttacgctg tgacaatagt taattaatta
     5401 agaagtttaa atgataccat tcatgctttt ataaatacct tttaagattt ttgatggtag
     5461 tgtaaaatat cttgtgtaaa cacacaaaaa ggaataaatc ctgtatagta gagttgcgaa
     5521 acttactaga aagagattta ttcccatgac tcagtttacc acagaattac ttaacttcct
     5581 agcacaaaaa taagatattg atgaattttt tcgctcctct ttagaaatag ctatgaatga
     5641 cctcttgcaa gttgaactat cagcttttct tggttatgaa ccttatgaaa aagaaggtta
     5701 caacacaggt aatagccgta atgggactta ttctcgtcag tttgaaacaa agtatggctt
     5761 agtcaattta atcatcccaa gagatcgaaa cggcgagttt tcaccagttt tattaccatc
     5821 ttatgctaga cgagatgacc acttggaaga gatggtgatc aaactctatc agactggcgt
     5881 tacgacacgc gaaatcagtg acattattga gcgcatgtat ggtcaccact tgtttctgag
     5941 tggaagcctc gttacaaaaa ggttatgaca tcacttgaaa caacggaaaa tctcctaact
     6001 ttctatcaat ttccatatta catttggtct agtatttact ctacaaactt gattgaatca
     6061 ctcaataaag aaatcaagcg acaaagcaag aaggagggtg gtttttccaa atgaagaagc
     6121 cttagaacga tgtcttgtaa gtattttcga agactataat attaagttcg gagctcgtat
     6181 tcataaagga ttcggagtat gttttgacac acttgatagc ttatttgact aatattcaaa
     6241 aactctactc ttgtgtttac acaacattat tgacagtctc aattaatcta tatcccttta
     6301 atattattaa tttgtaatag tacttagtaa gacataaaca aatatcacaa ttaaaattac
     6361 tttacttgca ttttctctta cctctttatc cttcaaaaaa atttgggaaa gtattagaca
     6421 aattaaaccg agtattataa ctataatcat atcattgata agctgcaatt ccagatgcta
     6481 cacatacaaa tgctgccgca cctccagtta ttggacctcc tgctgcagct ataagcatcc
     6541 cacctaagca ccctccgata gcagatccaa tatttccttt tccaccagaa atatttacta
     6601 attctttaaa ctgtaattct aaaaaattat ccatttttca cctcaaaatt attttccatt
     6661 aaataacata ccaccaacgc aagttaatcc acccgcaata gcacctacat gtgcacctgc
     6721 aaacgcacct gcaatagcac ctgaagtaat accaccaatt aaatgatttt tacaatctgt
     6781 agcactataa cctccaataa tatggaacaa ctcttctttt tgtatttctt ctgcaaattt
     6841 tatcataact ttaaaaacct ttctaaaatg ttatattcat cttgttgcaa ctttgacaaa
     6901 ctaaatatag catattatta taaaaaattt ttttctatgt cgtgaatggt tgtataaaca
     6961 tctcaaataa actttttaac atattagaga ctgtcaataa tgttgtgtaa acacaagagt
     7021 agagtttttg aatattagtc aaataagcta tcaagtgtgt caaaacatac tccgaatcct
     7081 ttatgaatac gagctccgaa cttaatatta tagtcttcga aaatacttac aagacatcgt
     7141 tctcttttag gatatatgat atctatctat attaactatt acctcaagag atactttcta
     7201 aaaactcaaa ttattagtgg gaaaaactta gattataaat acaaatgtca ggtttatttt
     7261 atcataaatc atactttttt aaatctgagc aagtatatga ccttctaaac gaaatacgta
     7321 tgcgttatcc tcatgctcat attattctaa tcggaaatca tattaattac gaggaaatat
     7381 ttaaaaatca ttatcgagtt tttggtgtta ttgacacaac aagtcatgaa tcattaaggt
     7441 ccattcgaga ccaaattcaa ctttacttag acgaacttta caattcgaat aatggatagg
     7501 cacttcatca attcttttag gaaatggttc ttgtcacccc ttccaagtct attgccacag
     7561 ttaggttcaa cttgcctaag tcatgcatgg ctaggtggca attcgtcata actctaaaac
     7621 aaatgtttga tgtctgctag tatcaattgg aaagaaaatc ctgttaacat ttaacaaaat
     7681 tattcagact taatcagaaa aattagaagc aaaaaaagag agcataagct ctcgaaacat
     7741 cgtctttcaa aaattttttt aacaagccaa tctccaaaaa tactccaact gtcctagaga
     7801 gttcttaatg ccccatagca aagctatcac taaaatattc ctctaacatc aggcgatgtt
     7861 tggctgagac actctctaac tctgctatct cctcatgaga gaaaaactgt aacttgagcg
     7921 tttcctcatt atgaaagtta tcaatagcca tatcagaaac agccgttagt tcatagataa
     7981 agacaatagt ctgaacagca tcaccgttgg gataaacttc ctcaaaatga gtgtaaacat
     8041 tcagtaagcg aacagcttca acctcaattc cggtttcctc taagaactca cgtttgcaag
     8101 tctctaaaga gctctcaccc agttccatag tgccaccagg aatagcccag gttttcttat
     8161 caccacgcaa ctgcatcagc accttgccgt catcattggt caaaatccca ccagcaaagt
     8221 taagaataat cttatcatgc ccaactttgg agcggatata agaaatgtag tcttgtggca
     8281 tatgatctcg ctttactatg ttagtattct ttctcattat agccaaaatc agctaaatca
     8341 agctttttat cccgccagtt tttcttgact ttgacccacg tttctaagta gaccttgtcg
     8401 cctaacatga gttcaatatc acggcgcgcc atttttccga tttttttgag catggctcct
     8461 tgttttccaa tgatgatgcc tttttggcta tcgcgctcca ccataatggt tgcacggata
     8521 tgcactttat cagtctcttc atctcgcttc atggattcga caacaacagc tacggaatgt
     8581 ggaacctctt gttgcgtcaa atgcagtacc ttctcacgaa ccatttctga cacaaggaaa
     8641 cgttctggat gatcagtaat ctgatcttct ggaaaatatt ggaaaccttc ttcaagatta
     8701 tcagttaata acttgatcaa agttggcaca ttattacctt ctagggcaga aattgggacc
     8761 acttctttaa aatccatttg gctacggaag tcatcaatct gttccaaaag ttggtctgga
     8821 tgaaccttat caattttgtt gatgactaaa ataacaggaa tcttagctgc ttttagccgc
     8881 tcgataatca tgtcgtcacc cttaccacgc ttttcatcgg caggaaccat gaaaagaacc
     8941 gtctcaactt cacgtaaagt actataagca gattcaacca taaaatcacc aagggcggtc
     9001 tttggtttat gaatacctgg cgtatcaata aagacaattt gttctgtttc tgtggtgtaa
     9061 atgcccataa ttttattacg ggtggtctga gccttgtcac tcatgatggc aattttttgc
     9121 cccatgacgt gatttaaaaa ggtggatttg ccaacatttg gacgaccaag aatggctaca
     9181 aagcctgatt taaacataga taattacggg ccttaacggt agggcttcag atcaaacttg
     9241 tcactaagct taaccgtctt gtcctttcct tattctccta tgccatcatc acataggata
     9301 gcccattcct ttctttttaa tgaacgaaaa tattccaaat tttaggaata aaaataataa
     9361 gtcctgtcaa gacagcatag ccagaaatca tcaaaacagc acctgctgcc atatccttgg
     9421 catttttagc tagcatagaa aagtgatagt cactagccaa atcaactaca ttttcaatag
     9481 cagaattgac aatttctaag gtaatgacca aaaaaatggc tagaagtaaa aataaccatt
     9541 cgatggctga aatgctaaaa aataaaccag caacacaagc taaacaggca gacaaaaggt
     9601 gacttctgag attgcgttct tccttaaaag ctgtgaaaac ccctgtcaag gcaaactcca
     9661 agcttgaggt aatggttctg tttttccatt tccgtttggt ggtattattg tcgtgtaagg
     9721 ccataagcag tcaaaatctc ttcctgtaag gtaaacatct ctttttcttc ttcaagggtg
     9781 tagtgatcat acccattaat atgtaagaat ccatgaacag ctaaaaatcc catttcacgc
     9841 tcaaaagagt gcccatattc ttgggactgc tcacgcgcct tatcaataga aataaacaat
     9901 tcgccaatat aagcatcaaa ctctgccatc atctctgcca aactaggatc agcagccaaa
     9961 tcctcttgac taaataaaat aggcgtttct ggcttgtatt ctagagaaat cacatctgtt
    10021 ggacgatcag tatctcggta ttctaagttg agttcatgac ttctttcatt ggtcacaaag
    10081 gtcacagaca tttctttttc ttctttgccc gtcttttgag cagcaaaatt gagcaaatca
    10141 agtgtttgct ccataatctc ttgcgaaact tgtcccgttt cgtcaatcat ctcgatatac
    10201 ataaggttaa ttgttaggga agggctgccc acctcatttg gtatgttggg cacccattat
    10261 tatctgacag ataatttccc ttatcctttc aatcagcatt tgttatttac ccattatacc
    10321 acaagcccct taaaaatggg aatgctaggt aaatcagggc ctatttggtt agaattgcct
    10381 ccacacgttt ttgcaagata ggtaataaat cctgctcaaa ccaggggttc ttagctaacc
    10441 acaattggtt tcttggagaa ggatgaacaa gtggaaaata gtctggcaag taatcctcaa
    10501 agtgacggac tgtttcagtt aaggttttgt aagccttgtt accaaggtaa aagtcctgag
    10561 catagcgacc aaccaaaatc gtcaattcaa cttctggcat caatgctctt aatggcgggt
    10621 gccacttagc cgcaaaacct tctctaggtg gtaagtcacc ggattttccc ttaccaggat
    10681 aatagaaatc catagggata atgccaaaca atcctgagtg gtaaaaggtg tcattgtcca
    10741 ctcctagcca gtctcgtaag cggatgccac tacgatcatt ccagtaaagt ttggtttctt
    10801 gagctactat tccaggagct tggccaacaa taataatcct agctgtttga ggagcatcat
    10861 ataaaggaaa aatacctctt tcggtatagg agagattagc ctcatctgcc ataatagctt
    10921 tggttaaatc atccatagaa tcctccttaa aaaggccata ttaaacatca tcgtaaaagt
    10981 cttagtgctc ttgaccgatc acagggtatt ttgtcaagcc tggctggtaa tcagcttcaa
    11041 cgtcactagc cttgggtttt agtaagtcct tcgtctcctc tgaggacgtt tcataagcct
    11101 tgataatatc agccacaaca ggatgtcgga caacatcctt agcagataaa tagacaaaat
    11161 caatctgttt aataccttgt aatttctgag tagcatcaat caaaccagac ttgacatttc
    11221 gagggagatc aatctgactc gtatctccat tgacaatcat tttagaatta aaaccaagac
    11281 gggtcaaaaa catcttcatt tgcatgatgg tcgtgttttg agcctcatct aagataacaa
    11341 aagcgtcatc cagagtacga ccccgcatat aagctaacgg agcaatctca atcacatctc
    11401 gttccatcag gcgtgtggtt tgttccttgc ctaaaatgtg ataaagggca tcgtaaaccg
    11461 gtctgaggta aggatccacc ttttctttca aatcgcctgg taaaaaacct aggctttcac
    11521 cagcttctac cgcaggacgc gtcaaaataa tacgctttac ctgtcctctt ttcagagcag
    11581 tgactgcaag cgtaacagct aagaaggttt tccctgtccc tgcaggtcct acaccaaaaa
    11641 ccacatcatg acgtttgaca ctatcaacat aggttttttg acctaatgtt ttgactcgta
    11701 ttgccttacc atagttatct ttaatgattt cttcttcata aagcgccata aattggtcaa
    11761 tctgatgaga ttctgccata gataaagctg tcaccacatc tgatgtgttg accaccatgc
    11821 cacgacccac tagaaccagt agggct
//
