LOCUS       NZ_CP028834            10513 bp    DNA     linear   CON 25-MAY-2024
DEFINITION  Streptomyces sp. M2 chromosome, complete genome.
ACCESSION   NZ_CP028834
VERSION     NZ_CP028834.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M2
  ORGANISM  Streptomyces sp. M2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8718751)
  AUTHORS   Worsley,S.F., Murrell,J.C. and Hutchings,M.I.
  TITLE     The chemical ecology of Streptomyces-plant root interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8718751)
  AUTHORS   Worsley,S.F., Murrell,J.C. and Hutchings,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2018) Biological Sciences, University of East
            Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom
            (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.99x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004104505.1-RS_2024_05_25
            Annotation Date                   :: 05/25/2024 03:18:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,020
            CDSs (total)                      :: 7,927
            Genes (coding)                    :: 7,816
            CDSs (with protein)               :: 7,816
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 39 of 111
            Pseudo Genes (incomplete)         :: 91 of 111
            Pseudo Genes (internal stop)      :: 7 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:04:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5359739
            Orig. end                         :: 5370252
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028834.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Matthew Hutchings,
            School of Biological Sciences, University of East Anglia, Norwich
            Research Park, Norwich, NR4 7TJ.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10513
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="18"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10513
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10513
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5513](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5513
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(355..675)
                     /locus_tag="DBP13_RS24745"
     CDS             complement(355..675)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435075.1"
                     /locus_tag="DBP13_RS24745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_108014767.1"
                     /transl_table=11
                     /translation="MRNKVTAISALAAGIVLAAGGAAAADDNNTTVNGGGGFSSSPVVV
                     NAPQQGLAVVNGTVVDGRCIAPWSNGAVLGVILAPNSHYAACNTDKVDQSQNAPYIGGL
                     IL"
     gene            complement(736..1053)
                     /locus_tag="DBP13_RS24750"
     CDS             complement(736..1053)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435076.1"
                     /locus_tag="DBP13_RS24750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_108014766.1"
                     /transl_table=11
                     /translation="MRNKVTAISALAAGVVLAAAGTAAADNCTTVKGGNGFSSSPVVVN
                     APQQGAVVVNGTVADLRCVAPWSNGAVLGGVLATNSHYAACNTADIDQSQNAAYKGGLL
                     L"
     misc_feature    1043..1060
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.32"
                     /tool="antismash"
     gene            complement(1119..1439)
                     /locus_tag="DBP13_RS24755"
     CDS             complement(1119..1439)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435077.1"
                     /locus_tag="DBP13_RS24755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_108014765.1"
                     /transl_table=11
                     /translation="MPRKFAVITALTLGAVVSSVGVASAHDNNTEVKGGGGFSSSPVVV
                     NAPQQGVLVVNGTVVDGRCIAPWSNGAVLAGVLAPNSHYAACNTDEIDQTQNAAYKGGL
                     LL"
     gene            complement(1884..2273)
                     /locus_tag="DBP13_RS24760"
     CDS             complement(1884..2273)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499736.1"
                     /locus_tag="DBP13_RS24760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_241662384.1"
                     /transl_table=11
                     /translation="MKKAVFTTAALAIGAGLVGGSAVAASAATAAPAPSAGVLGLMSPT
                     KVTPAVVPAADVVTRIARDGKIANGSAFDEKPSGGASGVYLDGMDPSAAKKPTIPVNNP
                     AGRGSVQVHPLQAPTAAGLGSLLGK"
     gene            2551..3306
                     /locus_tag="DBP13_RS24765"
     CDS             2551..3306
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435079.1"
                     /locus_tag="DBP13_RS24765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-glucanase"
                     /protein_id="WP_128802865.1"
                     /transl_table=11
                     /translation="MTADSSKFPPIASNPAATGTTWSALGKSLVWAEEFDAPIEWGTKW
                     VGDRTSAYRYANHNPDDNKLDWLDPGCVTVSGGVATFTATPAAHTLENGLQAWRTGLLT
                     TEYTDEGFRVRTGDCAEVRLRLPSGNGAWPALWTWKDGGNEVDTFEYHPDNPHLLELCN
                     RVNDACTYYTDAHAVAPGRWVTVASHYGEHSVDWYVDGERVFRDGTGVGGDWSAYLILN
                     LSLCAGEHHPAPSAPGPVTFAVGHLRVYR"
     gene            3413..4066
                     /locus_tag="DBP13_RS24770"
     CDS             3413..4066
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435080.1"
                     /locus_tag="DBP13_RS24770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_108014763.1"
                     /transl_table=11
                     /translation="MRGLSARRIAATALSATLLVGITGPAAVAADTARDHSHAASRGAP
                     VPGADALLAQVKSLGDLGSILTPVSALLNAVLKADNGQLSADEAQKLVTAVKDAITAIT
                     APAAATPGGVAKPVLPATSTVLPKPHSAAKQAAHAKTPAPKDLKADALAGLQKALDTLL
                     AAVTSGDASKVVPAVTGVLTAVVNFLAATLLNSGLPAPNLPGLPALPSLPLPVG"
     gene            4349..4849
                     /locus_tag="DBP13_RS24775"
     CDS             4349..4849
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065676.1"
                     /locus_tag="DBP13_RS24775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5949 family protein"
                     /protein_id="WP_128802866.1"
                     /transl_table=11
                     /translation="MTSTPSQTHPFDIAELGTLVLLPWSSEAPDGADMPYLLAYSLGDA
                     EGGPEMTSAAVAGLLASHGLMVGGRLVDGTAGTGLAITLHVEDGQAVVGMPELGARCAA
                     PPAWLTAVGERGYAYLVFTTRPWPEGGPGLPIDPATLTAFVGAEQTLRAAAHVILPAQG
                     SRG"
     gene            5001..5513
                     /locus_tag="DBP13_RS24780"
     CDS             5001..5513
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435082.1"
                     /locus_tag="DBP13_RS24780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_108014761.1"
                     /sec_met_domain="melC (E-value: 2.4e-43, bitscore: 146.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVGADGAATGAQPGAEDRAGSGPERRPPWVIRRGLLASAAALTL
                     APLVGASRASGSADASEGSAETREPAFDEVYRGRHIQGRPLAASAGGPEDRAWQVTVDG
                     HPLHLMRRADGTWLTMVDHYSSYRTSLAATRAAVDELGPGQRLRDLAPGPMGERYGDMG
                     DHHGVRA"
     gene            5497..6366
                     /locus_tag="DBP13_RS24785"
     CDS             5497..6366
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="DBP13_RS24785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_128802867.1"
                     /transl_table=11
                     /translation="MAYVRKNVTKLTREEKRRFVNALLELKWRGEYEEFVRLHVAYYTA
                     DGETGLRAAHMGPSFLPWHRRFLLDLEQALQRVDPSVTVPYWDWTRDRSAKSAPWTDDL
                     LGGNGRPGDRQVVSGPFAYAQGHWPIREGVTDGVYLTRDLGRAHAPMALPTKKELESAL
                     AEKVYDTSPWDSTCTRGFRNKLEGWGTGRDDVAWRNHNRVHRWVGGVMLGGASVNDPAF
                     WLHHAFVDLQWTRWQQRHKNAARYLPADPPGRDDEQHGRILARHESMPPWNVTPDEMED
                     VSAIYRYA"
     gene            complement(6416..7369)
                     /locus_tag="DBP13_RS24790"
     CDS             complement(6416..7369)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976103.1"
                     /locus_tag="DBP13_RS24790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_164932391.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 6.2e-53, bitscore:
                     177.6, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRCAVLDDFQQVATELADWSPVADRVETVTFAEHFGTEDELAAAL
                     ADFDIVVTLRERVAFPASLLARLPRLKLLVASGMRNSVIDYAAAEAHGVTVCGTASTST
                     PPVELTWALLLGLARGIVEENNSLRSGGPWQQTVGADLHGRRLGLLGLGKIGSRVAQVG
                     LAFGMHVSAWSRHLTKEYADEVGVELAPSLAELLSSSDFVSVHLVLGDRTRGLLGPAEL
                     ALLKPTAHLINTSRAAIVDQDALLAALHEGRIAGAAVDVFDIEPLPADHPMRTAPRLLA
                     TPHLGYVSRANYETYYREAVEDIRAFLDGTPVRRLG"
     gene            complement(7443..8003)
                     /locus_tag="DBP13_RS24795"
     CDS             complement(7443..8003)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133311.1"
                     /locus_tag="DBP13_RS24795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_128802868.1"
                     /transl_table=11
                     /translation="MSYLRTFLPWIVFAAVPSGSWQWGALAALVVSLAVIAQQVRAGTG
                     LDALIIELGSAAFFAVLSVVAFADPHSGLHDYSAAVSSATLAAVAGLSLAVRRPFTLGI
                     AKRSTPPEVWGLKPFIRVNVVITAVWTAAFAVTAALLAAEAHAGHGHSTTALLIQIAGF
                     VVPMVFTVRYAAHARARAQQLAH"
     misc_feature    complement(8081..8091)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            8150..8662
                     /locus_tag="DBP13_RS24800"
     CDS             8150..8662
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1278: PadR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179981.1"
                     /locus_tag="DBP13_RS24800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_108014757.1"
                     /transl_table=11
                     /translation="MSLRHALLGLLSERTASGYDLLKLFDTSLANVWPATQSQIYTELT
                     KLAGSGLITVAAEGPRGRKEYAITDEGMAELRHWLTETKPQRTIRSDILLRVFFLGVLT
                     PEQARDYLNELTRISEEGAENLRQLSESVDWGDDNLSVHGRIALEYGLRFNTMRREWAE
                     WAAAQIK"
     gene            8768..9130
                     /locus_tag="DBP13_RS24805"
     CDS             8768..9130
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435085.1"
                     /locus_tag="DBP13_RS24805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_128802869.1"
                     /transl_table=11
                     /translation="MAADSGGYEDPSAEVLAEAAGAFGLLASSARLHIVWALAQGESDV
                     TRLADRVGGALPAVSQHLAKLKLAGLVRSRREGRRQIYFVDDPDVVTVVRVMVGQLAAR
                     SEGASAAGRHLHRAGG"
ORIGIN
        1 ggcatttccc gggggagggc cttggtggtg cgctgggcca aacgaaggaa ttccggtgac
       61 cattcgtggg acggtgccgt tgttccggtt ggaaacagcc cggcgccgac cgctttgcgg
      121 agcgccggcg ggaatgccgt aagcaaaggg cgccttttcc tccttgtcgg ccgccctcgc
      181 cgatcgcctc ccgtgccgcg gggcctgtca gcaccggaca caccggacgc cgacgggccc
      241 cgcggtcttt tgccgaactc gccctgaagc ggccgaagta cacaccactc ggcgacaaaa
      301 agccgatggc ggcccgccgg aattccggcg gaccgccatc gggcgaagta cagctcagag
      361 gatcaggcca ccgatgtacg gggcgttctg cgactggtcg accttgtccg tgttgcacgc
      421 ggcgtagtgc gagttcgggg ccaggatgac gcccaggaca gcaccgttgg accacggggc
      481 gatgcagcgg ccgtcgacga ccgtgccgtt gacgacggcc agaccctgct gaggggcgtt
      541 gacgacaacc ggggacgacg agaagccgcc accgccgttc acggtggtgt tgttgtcgtc
      601 ggccgcggcg gcgccgccgg ccgcgaggac aatgcccgcg gcgagggcgg aaatggccgt
      661 aaccttgtta cgcataagat gcgcctttcg gtggggcggt tggggatttc cgtgctccga
      721 attgctcgga gcaggtcaga ggagcagacc gcccttgtag gcggcgttct gcgactggtc
      781 gatgtccgcc gtgttgcacg cggcgtagtg cgagttcgtg gccaggacgc cgccgaggac
      841 ggctccgttg gaccacggcg cgacgcaacg gaggtcggcg accgtgccgt tgacgacgac
      901 ggcaccctgc tggggggcgt tcacgacgac cggcgacgac gagaagccgt tgccaccctt
      961 gacggtggtg cagttgtcgg ccgcagcggt gcctgccgcc gcgaggacga cgccggcggc
     1021 gagggcggaa atggccgtaa ccttgttacg catgagttgc gcctttcagg gctgaatgtg
     1081 ggtgatgcac ctgctccgat gacttgctcg ggacaggatc agaggagcag gccgcccttg
     1141 taggcagcgt tctgcgtctg gtcgatctcg tccgtgttgc acgccgcgta gtgcgagttc
     1201 ggggcgagaa cgccggcgag aacggctccg ttggaccacg gagcgatgca gcggccgtcg
     1261 acgaccgtgc cgttgacgac gaggacaccc tgctgggggg cgttcacgac gaccggggac
     1321 gacgagaaac cgccaccacc cttgacttcg gtgttgttgt cgtgggccga ggcaactccg
     1381 acgctgctga ccacagcgcc gagggtgagg gcagtgatga ccgcgaactt cctgggcaac
     1441 tcagttcctt cctcaattca cggttgcgca cgcttgcata ccaggaaacc ttgatatgcg
     1501 ggcataagtc acgggaagag cggctaaaca ggtctgattc catcggatgg cgcaatgcga
     1561 tgcgtccctg agggcctcac ggggaatgca gtcggaatcc tcgcggccca aatgaacgac
     1621 aatccgaaag gccaagcacg gcggctgccc ggagaaatgc cgcggttctt cggggcggca
     1681 cggcctgaac ggaacgggcg ccgacgcgtc tccggcccac cggaggacag aaggccggtg
     1741 ggccggaggg atgctgcatg gggcgcggcg tttttccttt gccggtcccg gtgcggtgtc
     1801 ggtcttgggc tcacggccgc gagggcgtcg tgaagccgtg agggctttcg ccggtgctgc
     1861 cgactcctaa ggggtgtgcc gcgtcacttg ccgagcaggc tgccgagacc ggctgccgtc
     1921 ggtgcctgca gcgggtgtac ctggacggag ccccggccgg ccgggttgtt gaccggaatc
     1981 gtgggcttct tggcggcgct ggggtccatg ccgtccaggt agacgccgga ggcgccgccc
     2041 gacggcttct cgtcgaaggc gctcccgttg gcgatcttgc cgtcacgggc gatacgggtg
     2101 acgacgtcgg cggcgggaac caccgccgga gtcaccttcg tcggggacat cagaccgagc
     2161 acaccggccg agggggccgg agccgcggtg gcggcggagg ccgcgacggc ggagccgccg
     2221 acaaggcccg caccgatggc caatgcagca gtggtgaaaa ctgccttctt catgattcac
     2281 ctcggttgaa agttacctct gatccgacgg ctggagaaca tagcagtcca tccgcactgc
     2341 aaaagcgacg cgttactcca tacgcatgaa ttcagcggga gcaggcatag gcgtgggcgc
     2401 ggtcacggca gcgggatgca catcttcggt gaatcgactg atcgtatggt tcacccggaa
     2461 taggccgtac gggtcatcgg ggcgcggaat tccccggagt taccggcact tcggccgagg
     2521 aggccgccgg acggccctac cctcgcggcc atgaccgccg acagcagcaa gttcccgccg
     2581 atcgcgtcaa accccgcggc caccggtacc acctggtcgg ccctgggcaa gtccctcgtc
     2641 tgggcggagg agttcgacgc cccgatcgag tggggcacca aatgggtcgg tgaccggacg
     2701 agtgcctatc gctacgccaa ccacaacccg gacgacaaca agctcgactg gctcgacccg
     2761 ggctgtgtga ccgtctccgg cggcgtggcc accttcactg cgaccccggc ggcccacacc
     2821 ctggaaaacg gtctccaggc ctggcgcacc ggtctgctca ccaccgagta caccgacgag
     2881 ggcttccggg tgcggaccgg cgactgcgcc gaggtccggc tcagactgcc gtccgggaac
     2941 ggcgcctggc cggcgctgtg gacctggaag gacggcggca acgaggtcga caccttcgag
     3001 taccacccgg acaacccgca cctgctggag ctgtgcaacc gcgtcaacga cgcctgcacg
     3061 tattacaccg acgcccacgc ggtcgccccg ggccggtggg tgaccgtcgc cagtcactac
     3121 ggcgagcaca gcgtcgactg gtacgtcgac ggcgagcggg tcttccgcga cggcacgggc
     3181 gtgggaggcg actggtcggc gtatctgatc ctgaacctgt cgctgtgcgc gggcgagcac
     3241 catcccgcgc ccagcgcgcc ggggccggtc accttcgccg tcgggcacct tcgcgtctac
     3301 cgctgagcca tccgctcagc acgcgtcccc ctaaaggagc gcagcggttg caggggtgga
     3361 cgggcgaatt cacggtgggc tcaagatccg tcgcatctcg aaaggaacac ccatgcgtgg
     3421 tctgtccgca cggcgtatcg cggccaccgc cctcagcgcc acccttctgg tcggcatcac
     3481 cggtccagcc gccgtcgcgg ccgacacggc ccgggatcac agccatgccg cgtcccgcgg
     3541 cgcacccgtg cccggcgccg acgcgctgct ggcccaggtc aagagcctcg gcgacctcgg
     3601 cagcatcctg accccggtct ccgccctgct caacgcggtc ctcaaggcgg acaacggcca
     3661 actctccgcg gacgaggcgc aaaagctcgt caccgcggtg aaggacgcca tcaccgcgat
     3721 caccgcaccc gccgcggcca cccccggtgg tgtcgccaag ccggtcctgc ccgccacctc
     3781 cacggtgctg cccaagccgc actccgcggc caagcaggcc gcccacgcca agacgcccgc
     3841 tcccaaggac ctcaaggccg acgcgctcgc cgggctgcag aaggcgctgg acacgctgct
     3901 cgcggccgtc acctccgggg acgcgagcaa ggtcgtcccc gccgtcaccg gcgtgctgac
     3961 cgcggtcgtc aacttcctcg cggcgacgct gctcaacagc ggcctgcccg cgcccaacct
     4021 gccgggcctg cccgcccttc cgtcgctgcc gctgccggtg ggatgagccc ctggtgagcc
     4081 ctcggtgacg ccctgacggg ccgaagtgga tttcgtgcac tccgccgccg ggccggtcct
     4141 cgtgaccggt ccggcggcgg agctctgtcc ggccgcgtca ccgcggggat ctcatatgtc
     4201 ttccggccgc ggggtttccg ccccgggcgg cgatcgttgg gcgtggcgtc agctgtcttc
     4261 gacgcgctgc ccgcgacggt gccgggcgcc gtgaaaggaa cgccatgaag tccgtgaagt
     4321 ccgtgaaccc ggtgaacgag gacgcgcggt gacctccacc ccgagccaga cgcacccctt
     4381 cgacatcgcc gaactcggca cgctcgtact gctgccctgg agcagcgagg cccccgacgg
     4441 cgccgacatg ccgtatctgc tggcctactc cctcggagac gccgagggcg ggccggagat
     4501 gacgtccgcc gcggtcgcgg ggctgctggc gagccatggg ctgatggtcg gcgggcggct
     4561 cgtcgacggc accgccggga ccggcctggc catcaccctg cacgtcgagg acggacaggc
     4621 cgtcgtcggc atgccggagc tgggcgcccg ctgcgccgct ccgccggcct ggctcacggc
     4681 ggtcggcgaa cgcggctacg cctacctggt gttcaccacc cgcccctggc ccgagggtgg
     4741 gcccggcctg ccgatcgacc ccgcgacgct gacggcgttc gtcggtgccg agcagaccct
     4801 gcgggccgcc gcgcacgtca tcctgccggc ccagggttcg cgcggctgag ccgcgggcag
     4861 ggccgcgacg gtcccgctcc tccggcacgt gacgtccgtc cggggtgcgc gggcagggcc
     4921 gtatggccgt atcggtgtga ccggacggcc cgcgctgtgg ttatcgctgc ggacatcaga
     4981 tccgtccgag ggagtgaacc atggtggtgg gagccgacgg ggcagcgacg ggcgcgcagc
     5041 cgggggccga ggaccgggcc ggaagcgggc cggagcggcg gccaccgtgg gtgataaggc
     5101 gcgggctgct cgcctccgcc gcggccctga cactcgcccc cctggtcggc gcctcgcgcg
     5161 cctccggatc ggcggacgcg tccgagggct ccgccgagac gcgggagccg gccttcgacg
     5221 aggtctaccg cggccgtcac atccagggca ggccgctcgc cgctagcgcc ggcggccccg
     5281 aggaccgcgc gtggcaggtc accgtggacg gccacccgtt gcacctgatg cgccgggccg
     5341 acggcacctg gctgaccatg gtcgaccact acagctcgta ccggacctcg ctcgcggcga
     5401 cccgggcggc ggtcgacgaa ctcggccccg gacagcggct gcgcgatctg gccccggggc
     5461 cgatgggcga gaggtacggg gacatggggg atcaccatgg cgtacgtgcg taagaacgtc
     5521 accaagctga cgcgcgagga gaagcggcgg ttcgtcaacg cgctgctgga gctcaagtgg
     5581 cgtggtgagt acgaggagtt cgtacggctg cacgtcgcct actacaccgc cgacggcgag
     5641 accggtctgc gcgcggccca catggggccc tccttcctgc cctggcaccg gcgcttcctg
     5701 ctggacctgg agcaggcgct gcagcgggtc gatccgtcgg tgacggtgcc gtactgggac
     5761 tggacgcgcg accgctcggc gaagtccgcg ccctggaccg acgatctgct cggcggcaac
     5821 gggcgccccg gggaccgcca ggtcgtcagc ggtccgttcg cctacgcgca gggccactgg
     5881 ccgatccggg agggcgtcac cgacggcgtg tacctcaccc gggacctggg acgcgcccac
     5941 gcccccatgg cgctgcccac gaagaaggaa ctggagtcgg cgctcgccga gaaggtctac
     6001 gacacctcgc cctgggactc cacctgcacc cggggcttcc gcaacaagct ggaggggtgg
     6061 gggaccggca gggacgacgt cgcctggcgc aaccacaacc gcgtccaccg ctgggtcggc
     6121 ggcgtcatgc tcggcggcgc ctccgtcaac gaccccgcct tctggctgca ccacgccttc
     6181 gtcgacctgc agtggacccg ctggcagcag cggcacaaga acgccgcgcg ctatctgccg
     6241 gccgatccgc ccggccgcga cgacgagcag cacgggcgca tcctcgcccg gcacgagagc
     6301 atgccgccgt ggaacgtgac gccggacgag atggaggacg tgagcgcgat ctaccggtac
     6361 gcctgacggt cggtacatcc ggtaaatccg ggacgtccga ccggccggtg cgtgctcagc
     6421 ccaggcgccg tacgggcgtt ccgtcgagga acgcccggat gtcctccacc gcctcgcggt
     6481 agtacgtctc gtagttcgcc cgcgagacat agccgaggtg gggtgtggcg agcagccgcg
     6541 gagccgtgcg catcgggtgg tcggcgggca gcggctcgat gtcgaagacg tctacggccg
     6601 ctccggcgat ccggccctcg tgcagggccg cgagcagggc gtcctggtcg acgatcgcgg
     6661 cgcgcgaggt gttgatcagg tgggcggtgg gcttgaggag cgcgagttcg gcggggccga
     6721 gcaggccgcg ggtgcggtcg ccgaggacca gatggaccga gacgaagtcg ctgctcgaca
     6781 gcaactccgc cagggagggg gcgagttcga cgcccacctc gtcggcgtac tccttggtga
     6841 ggtgccggct ccaggcgctg acgtgcatgc cgaaggcgag gcccacctgt gcgacccggc
     6901 tgccgatctt gccgaggccg agcagtccga gccggcggcc gtgcagatcg gcgcccaccg
     6961 tctgctgcca gggtccgccg gagcgaaggg agttgttctc ctcgacgatg ccgcgggcca
     7021 ggcccagcag cagggcccag gtcagttcga cgggtggggt cgaggtgctc gccgtgccgc
     7081 acacggtgac gccgtgcgcc tcggcggcgg cgtagtcgat gacgctgttg cgcatcccgg
     7141 aggcgacgag cagcttcagc cggggcaggc gggcgagcag ggaggcgggg aaggcgacgc
     7201 gttcgcgcag ggtgacgacg atgtcgaagt cggccagggc cgcggcgagt tcgtcctcgg
     7261 tgccgaagtg ttcggcgaag gtgacggtct ccacccggtc ggccaccggc gaccagtcgg
     7321 ccagctcggt cgccacctgc tggaagtcgt cgagcacggc gcagcgcagc acgggggatc
     7381 ccttcgtcgg tgtgtccggc caggtcggca atgatcccgc atggcccggg tcgggggcgc
     7441 gatcagtggg cgagttgctg ggccctggcc cgggcgtggg cggcgtagcg gacggtgaag
     7501 accatcggga cgacgaagcc ggcgatctgg atgaggagcg ctgtggtcga gtggccgtgg
     7561 ccggcgtggg cctctgcggc gagcagggcc gcggtgacgg cgaaggccgc ggtccatacg
     7621 gcggtgatga cgacgttgac gcggatgaac ggcttgagcc cccacacctc cggcggggtg
     7681 ctgcgcttgg cgatgcccag ggtgaacggc ctgcgcacgg ccagcgagag accggcgacg
     7741 gccgcgaggg ttgccgagga caccgccgcc gagtagtcgt gcaggcccga gtgcgggtcg
     7801 gcgaaggcga cgaccgacag aacggcgaag aaggccgccg aacccagctc gatgatcagg
     7861 gcgtcgagtc cggtgcccgc cctcacctgc tgagcgatca cggccaagga gaccacgagg
     7921 gcggcgagcg caccccactg ccagctgccc gagggcacgg cggcgaagac gatccagggg
     7981 aggaacgtgc gcaggtagga catggggact ccgtccgggt tccgggggct acgggccttc
     8041 gggtttcact tgcgacatgt cgaaactaga aggtgggcga gtgacatgtc aagagtggaa
     8101 ggtcgtacgc tgacatgtcg atactggaag gtcggagtag ggtggatgca tgagccttcg
     8161 acatgccctg ctcggactgc tctccgagcg caccgccagc gggtacgacc tgctgaaact
     8221 gttcgacacg tcgctggcca acgtctggcc ggccacgcag agccagatct acaccgagct
     8281 gaccaagctc gcgggctccg gactgatcac ggtcgcggcg gaggggccgc gcggccgcaa
     8341 ggagtacgcc atcaccgacg agggcatggc cgagctgcgg cactggctga ccgagaccaa
     8401 gccgcagcgg accatccgca gcgacatcct gctgcgcgtc ttcttcctcg gcgtgctcac
     8461 gcccgagcag gcgcgggact acctgaacga gttgacccgg atctccgagg agggcgccga
     8521 gaacctgcgg cagctgtcgg agtccgtgga ctggggcgac gacaatctct ccgtccacgg
     8581 ccggatcgcg ctggagtacg ggctgcgctt caacaccatg cgccgggagt gggccgagtg
     8641 ggcggccgcg cagatcaagt gagctgtccg ggaggcccgt gacgggggct cggaaggggc
     8701 tcagaagggc acctagactg aaccggcgcg ctcggcgcgc tgatcagggc gggacgggag
     8761 aaggactgtg gcggcggaca gcggtggcta cgaggacccc tccgccgagg tgctcgcgga
     8821 ggcggccggc gccttcgggc tgctggcgtc ctcggcccgg ctgcacatcg tgtgggcgct
     8881 ggcgcagggc gagagcgacg tcacccggct cgcggaccgg gtcggcgggg cgctccccgc
     8941 ggtcagccag catctggcga agctgaagct cgccggtctg gtgcgttccc gcagggaggg
     9001 gcggcggcag atctacttcg tcgacgaccc cgacgtcgtg accgtggtca gggtgatggt
     9061 gggccagctg gcggcgcgtt ccgagggcgc gtccgccgcc ggacgccatc tccaccgggc
     9121 cggtggctga cagcagcgcg gccccggagg aggaccccgg ggcactcgaa cccggggaag
     9181 gaccggccgc ggtcgccggc tccgggcccc gggcggcgac cgtgctgcag accctgcggc
     9241 acctggacag cggtccccgt ggcctgacgg aggcgcaggc cgccgagcgg caggcccgcc
     9301 tcggacccca caccctgccg tcgcagcggg agacctcctg gcccacggcg ctgctgcgcg
     9361 gtctgcgcga cccgttcacc gccgtactgc tctgtctcgg cctggtctcc gcgaccgtcg
     9421 cctcctgggg caccgcctgc gtgatcctcc tgctggtcgc ggtcagctgc gggctgcgcg
     9481 cctccgggga gtaccgggcc gaccgctcca tggccgcact gcgggggctg gtcgccacca
     9541 cggccaccgt gctgcgcagg gatacggagc aactgccgcc gcacgtacgg gagatccccg
     9601 tggatgagct ggtgcccggc gacgtgatcc gcctcggccc gggggatctg gtccccgccg
     9661 acgtacgact gctgcgtgcg caggggctga cggttcatca ggccgcgctc acgggcgagt
     9721 cggcgccggt gagcaaggat gcggacgacc tggcggggga gggggcgagg acgggagcgg
     9781 gagacggcgg cggggaggcg gggtccgtcg gtcgtgccgg gccgggggcg gggctcgggg
     9841 agttcgaccg gcctcagctc tgcttccagg ggagcagcgt cgcgtccggg agcgccaccg
     9901 cggtcgtcgt ggcgaccggt gcgcgcacca ggttcgccgc cgatcacggc cgtacccgcg
     9961 cacggcggga ggccagtgcc ttcgaccggt ccgtgcacgg catctcctgg atcctcatcc
    10021 ggttcatgct gctgacccag ccgctggtcc tcgtggcggg cgccgcactg cgcggccgcg
    10081 ggcaggagac gttgccgttc gccgtcgcgg tggcggtcgg gctgacgccg gagatgctgc
    10141 cggtcatcgt caccctgtgc ctcgcgcgag gcgccgcgct gctggcccgg agccacgggg
    10201 tgatcgtcac acggctgccc gcgctgcacg acctcggcgc cgtcgacgtg ctgtgcctcg
    10261 acaagaccgg cacgctcacc caggaccggc cggtcctgca ccgcgccctc ggcgccgacg
    10321 ggcgggacga ccccgaggtg ctgcgctggg ccgcggtgaa cgcctggtgg accctgcaac
    10381 tggcggacct gcccgctccg gacgccctcg acgacgcggt gctggaggcg gccgacgagg
    10441 acgacgtcat ggcgtacgac ggtgtggccg cgctgccgtt cgatccgctg cggcggctgg
    10501 cgaccgcagt cgt
//
