LOCUS       NZ_CP028834            11308 bp    DNA     linear   CON 25-MAY-2024
DEFINITION  Streptomyces sp. M2 chromosome, complete genome.
ACCESSION   NZ_CP028834
VERSION     NZ_CP028834.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M2
  ORGANISM  Streptomyces sp. M2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8718751)
  AUTHORS   Worsley,S.F., Murrell,J.C. and Hutchings,M.I.
  TITLE     The chemical ecology of Streptomyces-plant root interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8718751)
  AUTHORS   Worsley,S.F., Murrell,J.C. and Hutchings,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2018) Biological Sciences, University of East
            Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom
            (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.99x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004104505.1-RS_2024_05_25
            Annotation Date                   :: 05/25/2024 03:18:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,020
            CDSs (total)                      :: 7,927
            Genes (coding)                    :: 7,816
            CDSs (with protein)               :: 7,816
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 39 of 111
            Pseudo Genes (incomplete)         :: 91 of 111
            Pseudo Genes (internal stop)      :: 7 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:04:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2001507
            Orig. end                         :: 2012815
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028834.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Matthew Hutchings,
            School of Biological Sciences, University of East Anglia, Norwich
            Research Park, Norwich, NR4 7TJ.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11308
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11308
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11308
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6308](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6308
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            520..1371
                     /locus_tag="DBP13_RS09255"
     CDS             520..1371
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA]"
                     /GO_process="GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432051.1"
                     /locus_tag="DBP13_RS09255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="WP_108006949.1"
                     /transl_table=11
                     /translation="MPDQGKTPLRKLLRVPAGGVDLSAYDAGGKPGGPKRKKAGLAATA
                     DLGKELAGLQDRLWASSTAGDPRRVLLVLQGMDTSGKGGTVKHVIGMLHPYGTHTKAFK
                     APTAEEREHHFLWRVEKEVPAAGEIGTFDRSHYEDVLAARVRELAPPEEIERRYDEINR
                     FEQRLADDRVTVVKCFLHISYEEQRRRLLRRLDRADKQWKFSPDDIDDRALWPAYQKAY
                     EIVLNRCNTPHAPWYLIPADRKWYRNWAVGRLLLEHLRALDPQYPKPGYDVEECRRRLL
                     GG"
     gene            1419..2273
                     /locus_tag="DBP13_RS09260"
     CDS             1419..2273
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063345.1"
                     /locus_tag="DBP13_RS09260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_164932142.1"
                     /transl_table=11
                     /translation="MIIQVRHMTALCALACAAGAALTACGAPDAPPAARPAPSATSAAP
                     SRPPTLAPGPSGLARVFEHGPRTQGKTVALTFDADMTADEGPRAEAGEHFDNPQLIATL
                     RRLKVPATLFMTGRWADEYPQEARSIGRDPLFEVANHSYSHYAFTGSCYGLPTFSVDRL
                     RADVERAYTALRKAGVPNAMPYFRFPGGCYDRKALKALAPTGATAVQWDVVSGDAFATD
                     ADAVAQQVLDGVRPGSVVVMHCTRSAAPVTEQAVRTIVPELRAKGFRFVKVSQLIGATS
                     GHR"
     gene            2292..2480
                     /locus_tag="DBP13_RS09265"
     CDS             2292..2480
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125244.1"
                     /locus_tag="DBP13_RS09265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_108006953.1"
                     /transl_table=11
                     /translation="MTDDSDDAYCLVDATRPPKADGPPYAECVQCRKPTEYPETYKGIT
                     LCPVCEWQEAQRTACSG"
     gene            complement(2489..3160)
                     /locus_tag="DBP13_RS09270"
     CDS             complement(2489..3160)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972873.1"
                     /locus_tag="DBP13_RS09270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter"
                     /protein_id="WP_108006955.1"
                     /transl_table=11
                     /translation="MSALLRYQAALLLRSQRWLPPVILYAAFLGIGVQGGQPVLDSLGY
                     AAAALLPVAAWLVRICVTVEPPAARACVAAASGPGRTHLTRLLVALLATTSLGTAATLL
                     VTLISDPETTDHHTRVPLLAATAAGLLATLACALLGTAVGALTNWPLLHSPGRAVPTML
                     LAALLSAVATGSPAQAAVSGLVTGSRSGEVPLPLLPLAAAAGLAAMAGAVACVLSSRRS
                     A"
     gene            complement(3157..4020)
                     /locus_tag="DBP13_RS09275"
     CDS             complement(3157..4020)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432055.1"
                     /locus_tag="DBP13_RS09275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_108006957.1"
                     /transl_table=11
                     /translation="MRRDLRLDGVGRRYGLRGPWVLRGVHLALAPGTLTRVEGANGTGK
                     STLLRLLAGIDAPTEGRVTGRPRTAYVPERFPSALPFTAHGYLTHLGAVHGLSRTAAAR
                     AADEWLERFGAAAHARTPMARLSKGSSQKVAVAQALLAEPELLVLDEAWTGLDTAARAE
                     LERAVAERTAGGGAVVFVDHDPRRLSGAPCTTYTVRDGALESRTREPSAPDDGPRVVVE
                     AHGPQGAQPPADALGALVSAEETAPGCHRLTVPASHSDVVLRALLTARPPWHVAGVHPL
                     GKDNHR"
     gene            4074..4790
                     /locus_tag="DBP13_RS09280"
     CDS             4074..4790
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122137.1"
                     /locus_tag="DBP13_RS09280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_206660140.1"
                     /transl_table=11
                     /translation="MSQDVTSSADSPFRSEPSERDQAPQFVLPLVVRIEKTAPPARTDA
                     LETAARAVLTLLSDERSLGDGEWAEAVRDWQDARIRKVVRRARGAEWRRAEALPGITVH
                     GRTAEVRVFPPVPLDGWPKDLVKLQVSGTDLDDPEPPAGADAALPVLWLNPGLDMSAGK
                     AMAQAGHAAQLAWWELSDEQRTAWRENGFALAVRTADPARWDELTSGGLPLVRDAGFTE
                     IAPGSCTVVADHPALR"
     gene            5001..6308
                     /locus_tag="DBP13_RS09285"
     CDS             5001..6308
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="DBP13_RS09285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_108006961.1"
                     /sec_met_domain="DUF692 (E-value: 1.3e-98, bitscore: 328.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAEAVERMPGIDWVEAVSENVCPGHLPESLLR
                     LRARGVTVVPHGVSLGLGGADRPDAGRLAALAERAEVLGSPLVTEHIAFVRAGGALTAS
                     PLLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALIAWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLASRVAPPGVLLERDENFPEPAELEGELAAIRTAVEEGAAQRAEA
                     AAVVDGGTRTASSASTDAARQRLALAQAALLSALVARTPVPEGFDRVRLGVQARALASK
                     RADVVAKVAPELPQILGEAYRSGFLAYAHGHPMTGGYRRDALDFAAHMLLDGHLAEGAV
                     RRELRAWWLERSGPVPRSQRPAVRAARAARRVLPHR"
     gene            6367..7140
                     /locus_tag="DBP13_RS09290"
     CDS             6367..7140
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432058.1"
                     /locus_tag="DBP13_RS09290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_108006963.1"
                     /transl_table=11
                     /translation="MRPRPPLQGRGILSGTGIIVIGLLATLMALIFPIWSYADRSGTGV
                     STLNAGSVPTRYGPLSALDRDFVRKVRLAGLWELPAGQEAEQKGTTQAVRTAGQHLAVG
                     HTFLDQRVRDVAGKLGLQLPNEPDAQQRQWLATLNAAHGTAYDRDFVNIARLSHGKVFS
                     LVAEVRAATRNSLVRSLADDANTTVLDHIKVLEATGDVDWNALAQDMAMASTPPPAGSP
                     APPGPTADPSPATPLSESPPPTYPLPPAATSPPPR"
     gene            7260..8048
                     /locus_tag="DBP13_RS09295"
     CDS             7260..8048
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432059.1"
                     /locus_tag="DBP13_RS09295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_108006965.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWATAGIACTRLCLAAVRAADGDAPARHHELTLYEA
                     AFLSGGPRRVADLTMVSMARQRRLLLAHTGWATVVDPRGRDEMERSVIGAIGPEGQSRI
                     APVRDATACADAVSGLADRLVDAGLAVPAGARTTVAAAVRQVQLASAAVLALGAVALLV
                     PARPDMPRGLVALWFALPLALTLSCLAIARVEVHPYSRWASPAGQRLLGALTRRTAGGD
                     DRTYLTSVAVRGVRAIGEPDLRAALSHRGQPWREPHIGGA"
     gene            8146..9795
                     /locus_tag="DBP13_RS09300"
     CDS             8146..9795
                     /NRPS_PKS="Domain: Abhydrolase_1 (112-486). E-value:
                     1.9e-19. Score: 62.2. Matches aSDomain:
                     nrpspksdomains_DBP13_RS09300_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432060.1"
                     /locus_tag="DBP13_RS09300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_128801664.1"
                     /sec_met_domain="PF00561 (E-value: 5e-23, bitscore: 80.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALHSAAGTLLLTALTLAPAGSAPAAPVAFDGAEQQGTAVAA
                     ARAGAAGIAFGKCADVKEMPGAMRCGTVSVPLDYAHPGGKQIKLTVTRVAATHKDPHNS
                     KRRVRRQGALVYNPGGPGGDGRYFPLVGLLPEWKRIAAAYDLVGYSPRGVGRSAPLSCE
                     DPGHFFKAPAQAPTHPSQSYKKARVAQAKAYAQGCAKRSGSGLKYFNSLNNARDLDVIR
                     AALGEPKLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPAPEQIWYRNNLEQSAAF
                     ESRWTDFRKWIAKHDDVYGLGRTVAAVQHSYAKASARLAAEPAGGKVGPAELQAAFLSA
                     GYYDDYWPTRAQALSAYLKGDPKPLIEQAGPATEAAAEAENSNAVYTAVECNDAPWPTD
                     FTVWDRDNTRLSRVAPFETWDNVWMNLPCAYWPTSRQQPLDVRTGPGELPPTLILAAER
                     DAATPYEGAQEMHRRLSGSVLVTERDAGTHGIAGGPNKCVNGYLDAYLLEGRTPDEQAS
                     CAPHAEPKPKPQADRARQTPLKEPLKGSTARP"
     aSDomain        8482..9603
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DBP13_RS09300_Abhydrolase_1.1"
                     /evalue="1.90E-19"
                     /label="DBP13_RS09300_Abhydrolase_1.1"
                     /locus_tag="DBP13_RS09300"
                     /protein_end="486"
                     /protein_start="112"
                     /score="62.2"
                     /tool="antismash"
                     /translation="ALVYNPGGPGGDGRYFPLVGLLPEWKRIAAAYDLVGYSPRGVGRS
                     APLSCEDPGHFFKAPAQAPTHPSQSYKKARVAQAKAYAQGCAKRSGSGLKYFNSLNNAR
                     DLDVIRAALGEPKLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPAPEQIWYRNNL
                     EQSAAFESRWTDFRKWIAKHDDVYGLGRTVAAVQHSYAKASARLAAEPAGGKVGPAELQ
                     AAFLSAGYYDDYWPTRAQALSAYLKGDPKPLIEQAGPATEAAAEAENSNAVYTAVECND
                     APWPTDFTVWDRDNTRLSRVAPFETWDNVWMNLPCAYWPTSRQQPLDVRTGPGELPPTL
                     ILAAERDAATPYEGAQEMHRRLSGSVLVTERDAG"
     gene            complement(10562..11293)
                     /gene="hemQ"
                     /locus_tag="DBP13_RS09305"
     CDS             complement(10562..11293)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="DBP13_RS09305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_108006969.1"
                     /transl_table=11
                     /translation="MSDDVPTTEATRIPNKGKLAKDLNEVIRYTLWSVFKLKDALPEDR
                     TGYADEVQELFDQLAAKDVTIRGTYDLSGLRADADLMIWWHAETSEQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRAHIPAFLADETPRNYISVYPFVRSYEWYLLPDEDRRR
                     MLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRASEARRHVR
                     TEIPFYTGRRKSVAELVEGWV"
ORIGIN
        1 cggggacgac ggcggtgact cgtccgcccc cgcgttcggc gcctcgaagc ggctcaccgt
       61 gtccagcacg gcgttcgccg acggtgccgc catcccgcgc cgctacacct gcgacggcga
      121 ggacatctcg cccccgctgt ccttctccgg ggtgccgtcc ggcacgaaga gcctcgcggt
      181 cctggtcgag gatcccgacg ccccgcgcgg ccccttcacg cactgggtgg tgttcgacgt
      241 ggcgccgtcg cagacgcgct ggccggcggg gcaggtgccg cgggccgccg tccaggggcg
      301 caacggtttc accaagaacg gctactcggg cccctgcccg ccccagggca agccgcacca
      361 ctacgtcttc tccggctacg ccgtcgaccg caccctccgc ctgccctccg gcgcctcggc
      421 cgacgacctg aagcaggcgc tgaagggcca tgccctcgcc tacgggtcgc tgaccggacg
      481 ctacggccgc tgaccaccgc cgacgaaggg gaagaacccg tgcccgacca aggcaagacc
      541 cctctccgca agctgctgcg tgtgcccgcc gggggcgtcg acctgtcggc gtacgacgcc
      601 ggggggaaac ccggcggccc gaagcgcaag aaggcggggc tggccgccac cgccgacctg
      661 ggcaaggagc tcgccgggct ccaggaccgg ctgtgggcgt cgagcaccgc cggtgatccg
      721 cgacgggtgc tgctggtgct gcagggcatg gacaccagcg gcaagggcgg caccgtcaag
      781 cacgtcatcg gcatgctcca cccgtacggc acccacacca aggcgttcaa ggcgcccacg
      841 gccgaggaac gggagcacca cttcctgtgg cgcgtcgaga aggaggtccc cgcggcgggc
      901 gagatcggca ccttcgaccg ctcccactac gaggacgtcc tcgccgcccg ggtccgcgaa
      961 ctcgccccgc ccgaggagat cgagcgccgc tacgacgaga tcaaccgctt cgagcagcgc
     1021 ctggcggacg accgggtcac cgtcgtcaag tgcttcctgc acatctccta cgaggagcag
     1081 cgccggcgtc tgctgcgccg cctcgaccgc gccgacaagc agtggaagtt cagccccgac
     1141 gacatcgacg accgcgcgct gtggcccgcg taccagaagg cgtacgagat cgtcctgaac
     1201 cggtgcaaca ccccgcacgc cccctggtac ctcatcccgg ccgaccgcaa gtggtaccgg
     1261 aactgggcgg tgggccggtt gctcctggag catctgcggg ccctggatcc gcagtacccg
     1321 aagccgggct acgacgtgga ggagtgccgg cggcggctgc tcggcgggtg agcgtgccgg
     1381 tccgctgatt gaccgttttg caccgttatg ttcatgcggt gatcattcaa gtacgccaca
     1441 tgaccgccct gtgtgccctc gcctgtgcgg cgggcgccgc gctcaccgcc tgcggcgcac
     1501 ccgacgcgcc tcccgccgcc cgcccggcac cctccgccac ctccgccgcc ccgtcccggc
     1561 cgcccacgct cgcgcccggc ccctccgggc tcgcccgcgt cttcgagcac ggcccgcgca
     1621 cccagggcaa gaccgtcgcg ctcaccttcg acgccgacat gaccgccgac gaggggccgc
     1681 gggccgaggc cggcgagcac ttcgacaatc cgcagctgat cgccacgctg cggcggctca
     1741 aggtaccggc gaccctgttc atgaccgggc gatgggccga cgagtatccg caggaggcac
     1801 gctccatcgg ccgcgacccc ctcttcgagg tggccaacca ctcctacagc cactacgcct
     1861 tcaccggcag ctgctacggc ctgccgacct tctccgtgga ccggctgcgc gcggacgtgg
     1921 agcgggcgta caccgccctg cgcaaggccg gagtgccgaa cgcgatgccg tacttccgct
     1981 tcccgggcgg gtgttacgac cgcaaggcgc tcaaggcact ggccccgacc ggagccaccg
     2041 ccgtgcagtg ggacgtggtc agcggggacg cgttcgcgac ggacgccgac gcggtggccc
     2101 agcaggtgct ggacggggtg cggccgggct ccgtcgtcgt catgcactgc acccgcagcg
     2161 cggcaccggt caccgagcag gccgttcgca cgatcgtgcc cgaactgcgc gcgaagggct
     2221 tccggttcgt gaaggtgtcc cagctgatcg gcgccacgag cggacaccgc tgaccggctt
     2281 accctgaagg catgaccgac gacagcgacg acgcctactg cctggtcgac gccaccagac
     2341 cccccaaggc cgacggcccg ccgtacgcgg aatgcgtcca gtgccgcaag ccgaccgagt
     2401 acccggagac gtacaagggc atcaccctgt gcccggtctg cgaatggcag gaggcccagc
     2461 ggacggcctg ctcgggttga tcggctactc aagccgaccg ccgcgacgac agcacgcagg
     2521 ccaccgcgcc cgccatcgcc gccagcccag ccgccgcggc caacggcagc agaggcagcg
     2581 ggacttcgcc cgaacgcgaa cccgtgacca gaccgctcac cgctgcctgc gccggggaac
     2641 ccgttgccac tgctgacagt agggccgcga ggagcatggt ggggactgcg cggccggggg
     2701 agtgcaggag cggccagttg gtgagggcgc cgacggctgt gcccaggagg gcgcaggcca
     2761 gggtggccag taggccggcc gcggtcgccg cgagcagggg tacgcgtgtg tggtggtccg
     2821 tggtctccgg gtcgctgatg agcgtcacga ggagggttgc ggccgtgccg agtgacgtcg
     2881 tggcgaggag cgccaccagc agccgggtca ggtgcgttcg gccggggccg gacgcggccg
     2941 ccacgcatgc gcgggccgcg ggcggctcga cggtcacgca gatccgcacc agccaggcgg
     3001 cgaccggcag cagggccgcc gccgcgtacc ccagcgagtc gagcaccggc tgcccgccct
     3061 ggacgccgat gccgaggaac gccgcgtaca ggatgaccgg cggcagccag cgctgcgagc
     3121 gcagaaggag ggcggcctgg tagcgcagca gggcgctcat cggtggttgt ccttgccgag
     3181 ggggtgcacg cctgccacgt gccacggcgg gcgggccgtc agcagggcgc gcagcacgac
     3241 gtccgagtgg gacgcgggga cggtgaggcg gtggcatccc ggcgcggtct cctcggccga
     3301 gacgagcgcg cccagtgcgt cggcgggcgg ctgcgcgccc tgcgggccgt gcgcctcgac
     3361 cacgacccgc gggccgtcgt ccggtgcgga gggctcgcgc gtacggctct ccagcgcgcc
     3421 gtcgcgcacg gtgtacgtgg tgcacggcgc ccccgacagg cgccgcggat cgtggtccac
     3481 gaacaccacg gccccgcccc cggcggtgcg ctcggccacg gctcgttcga gctccgcgcg
     3541 ggccgccgtg tccaggcccg tccatgcctc gtcgaggacg agcagctccg gctcggcgag
     3601 cagcgcctgc gccacggcga ccttctggct gctgcccttc gagaggcgcg ccatcggggt
     3661 gcgggcgtgg gcggccgcgc cgaagcgctc cagccactcg tcggcggcgc gggccgcggc
     3721 ggtccgggac agcccgtgga cggcgccgag gtgggtgagg tagccgtgcg cggtgaaggg
     3781 aagggccgac gggaagcgtt cggggacgta ggcggttcgg gggcggccgg tgacacggcc
     3841 ctcggtgggg gcgtcgatgc cggcgagcag ccgtagcagg gtggacttgc cggtgccgtt
     3901 cgcgccctcg acgcgcgtca gcgtgcccgg ggccagggcg agatggaccc cgcgcaggac
     3961 ccaggggccg cgcaggccgt agcggcggcc gacgccgtcc agccggagat cgcgtcgcat
     4021 gcgggcatcc tcgcaaagag gcaccgcaac gcgccccttt agggttggcc ggtgtgagcc
     4081 aagatgtgac gtcctccgcc gacagcccct tccggtccga gcccagcgag cgcgaccagg
     4141 caccccagtt cgtgctcccc ctcgtcgtac gcatcgagaa gacggcgccg cccgcgcgta
     4201 ccgacgcgct ggagaccgcc gcccgtgccg tgctgacact gctcagcgac gagcggtccc
     4261 tgggggacgg cgagtgggcc gaggcggtgc gggactggca ggacgcccgg atccgcaagg
     4321 tggtgcggcg ggcccgcggc gcggagtggc ggcgggccga ggcgctgccc ggcatcacgg
     4381 tgcacgggag gacggccgag gtgcgggtgt tcccgccggt tccgctcgac ggatggccca
     4441 aggacctggt caagctgcag gtgtccggaa ccgatctcga cgacccggag ccaccggcgg
     4501 gcgcggatgc agcgctgccc gtcctgtggc tcaaccccgg cctcgacatg tcggcgggca
     4561 aggcgatggc gcaggcgggc catgccgccc aactggcctg gtgggagctg tcggacgagc
     4621 agcgcacggc ctggcgcgag aacggcttcg ccctcgccgt acgcactgcc gacccggccc
     4681 gctgggacga actgacctcc ggcgggctgc cgttggtgcg cgacgcgggc ttcaccgaga
     4741 tcgcccccgg ctcgtgcaca gtggtggccg atcacccggc gctgcgctga acgcagcagc
     4801 agggcacccc ggagtgcgtc gccaacctca aatcttgctc tgaaggtgcg cggcgcggga
     4861 ttcggctccc ggcggcgggg ccatacctcc gtcacgtcga cgccgacgta tcgaaggaga
     4921 cgtatcgccg aggacgatca cggggtcgcc ggatcgaacg cagccggact tcgggacacg
     4981 gccgaggagg gggacacacc atggagcgac tgggaacggg catcggttgg cggccggaga
     5041 tcgcggaggc cgtggagcgc atgccgggca tcgactgggt cgaggccgtg tccgagaacg
     5101 tgtgcccggg acacctcccc gagtcgctgc tgcggctgcg cgcccgcggc gtcaccgtcg
     5161 tcccgcacgg cgtctcgctg ggcctcggcg gcgcggaccg gccggacgcc gggcggctcg
     5221 cggcgctcgc cgagcgggcc gaggtgctgg ggtcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtacgggc gggcggcgcg ctgaccgcct ccccgctcct ggaggccggg catctgctgc
     5341 ccgtgccacg cacccgggac gccctcgacg tgctgtgcga gaacgtccgc atcgcacagg
     5401 acgccctgcc ggtgccgctc gccgtggaga acatcgccgc gctgatcgcc tggccgggcg
     5461 aggagatgac ggaggggcag ttcctgtacg agctcgccga ccggacgggc gtacggctcc
     5521 tgatcgacgt cgccaatctg cacaccaacc acgtcaaccg cggcgaggac ccggccaagg
     5581 cgctcgccga gctgcctttg gaggccatcg cgtacgtcca tgtcgcgggc ggtttcgagc
     5641 gcgacggcgt ctggcacgac agccacgccc acccggtgcc gcggccggtc ctggacatcc
     5701 tgaccgacct cgcctcgcgc gtcgcaccgc cgggcgtgct gctggagagg gacgagaact
     5761 tccccgaacc ggcggagctg gagggcgagt tggcggcgat ccggaccgcc gtggaggaag
     5821 gggcggccca gcgggccgag gccgcggcag tcgtcgacgg cggcacccgt acggcctcct
     5881 ccgcgtccac cgacgccgcc cgtcagcggc tcgcgctcgc ccaggccgcc ctgctgtccg
     5941 cgctcgtcgc ccggacgccc gtgccggagg ggttcgaccg ggtgcggctg ggggtgcagg
     6001 cgcgggcgct cgcctcgaag cgggcggacg tggtggccaa ggtcgcgccc gaactgcctc
     6061 agatcctggg cgaggcgtac cggtccggct tcctcgcgta cgcccacggg cacccgatga
     6121 cgggcggcta ccgccgggac gcgctggact tcgccgcgca catgctgctc gacgggcacc
     6181 tcgcggaggg cgccgtccgg cgggagctca gggcgtggtg gctggagcgg tcgggtccgg
     6241 tgccgcggtc gcagcggccc gcggtgcgtg cggcgagggc ggcccggcgg gtcctgccgc
     6301 accgctgagg ggaccggaag ggcggtcgaa ccccgcagta atattcggtg ccgtacagga
     6361 ggcaccatgc gaccgcgacc acctctccag ggacgaggca tcctcagcgg caccggcatc
     6421 atcgtcatcg gcctgctggc cacgctgatg gcgctgatct ttccgatctg gtcgtacgcg
     6481 gaccgttcgg ggaccggcgt cagcacgctg aacgccggga gcgtgccgac gcggtacggc
     6541 ccgctgtccg cgctcgaccg ggacttcgtc aggaaggtgc ggctggccgg gctgtgggaa
     6601 ctgcccgccg ggcaggaggc cgagcagaag ggcaccacgc aggccgtccg tacggcgggg
     6661 cagcacctcg ccgtggggca caccttcctg gaccagcggg tgcgcgacgt cgccggcaag
     6721 ctcgggctcc agctgcccaa cgagcccgac gcccagcagc ggcagtggct cgcgaccctg
     6781 aacgccgccc acgggacggc ctacgaccgc gacttcgtca acatcgcgcg gctgtcgcac
     6841 ggcaaggtgt tctcgctggt cgccgaggtc cgtgccgcca cccgcaactc gctggtgcgt
     6901 tcgctcgcgg acgacgcgaa caccaccgtg ctcgaccaca tcaaggtcct tgaggccacg
     6961 ggagacgtcg actggaacgc gctggcccag gacatggcca tggcgagcac acccccgccg
     7021 gccggctccc ccgccccgcc cggtccgacc gcggacccga gcccggcgac cccgctctcg
     7081 gagtccccgc ccccgaccta tcccctgccg ccggccgcca ccagcccgcc gccgcggtag
     7141 ccgcgatccc gccccgcatc aaccccgttt caaacggaac gtcacataca gacaacgctc
     7201 cgtcccggat cgtgaacagg gcatggcgtt cgaccgggca tctggcatag aaacgcggca
     7261 tgttctgggt ccttctcctg ctgctggcct gggccaccgc cggcatcgcc tgcacgcggc
     7321 tgtgcctggc cgccgtacgc gccgcggacg gcgacgcacc cgcccggcac catgagctga
     7381 ccctctacga ggccgcgttc ctgtccggcg ggccgcgtcg ggtcgccgat ctgaccatgg
     7441 tctccatggc ccgtcagcgt cggctgctgc tcgcccacac cggctgggcg acggtggtcg
     7501 acccgcgcgg ccgggacgag atggagcgct ccgtcatcgg ggccatcggg ccggaggggc
     7561 agagccgtat cgccccggtg cgggacgcca ccgcctgcgc cgacgccgtc tccggtctgg
     7621 ccgaccggtt ggtggacgcc ggtctggcgg tgcccgccgg cgcccggacc acggtggccg
     7681 cagcggtgcg ccaggtgcag ctcgcctcgg cggcggtgct cgcgctgggc gcggtcgcgc
     7741 tgctggtgcc cgcccggccg gacatgccgc gcggcctggt cgccctgtgg ttcgcgctcc
     7801 cgctcgcgct gacgctgagc tgcctggcca tcgcccgggt cgaggtccac ccgtactcgc
     7861 gctgggcctc accggccggg cagcggctgc tcggcgccct cacccggcgc acggcgggcg
     7921 gtgacgaccg tacgtatctc acctccgtcg ccgtacgggg cgtgcgcgcg atcggcgagc
     7981 ccgacctgcg cgcggccctg tcgcatcgcg gccagccgtg gcgcgaaccg cacatcgggg
     8041 gcgcataggg ggcattgggg ccgaaccgcc gggtcggtgc ttgccttctc catacaccga
     8101 acgaaacatc ccttttgtcg ctgccatgcc tcgaagggat acgccatgag agctgccgcc
     8161 ctccactcgg ccgccgggac cctgctcctg acggccctga ccctggcccc ggccggcagc
     8221 gcaccggcgg caccagttgc cttcgacggg gccgagcagc agggcacggc ggtggccgcc
     8281 gcgcgggccg gggccgccgg catcgccttc ggcaagtgcg ccgacgtcaa ggagatgccg
     8341 ggggcgatgc ggtgcggcac ggtgtccgtc ccgctggact acgcgcaccc cggcggcaag
     8401 cagatcaagc tgaccgtgac cagggtcgcc gccacgcaca aggacccgca caacagcaag
     8461 cgccgggtgc gccgccaggg cgccctggtc tacaaccccg gcgggccggg cggcgacggc
     8521 cgctacttcc cgctcgtcgg cctgctcccg gagtggaagc gcatcgcggc ggcctacgac
     8581 ctggtcggct actccccgcg cggagtcggc cggtccgcgc ccctgtcctg cgaggacccg
     8641 gggcatttct tcaaggcgcc cgcacaggcg cccacgcacc cctcccagtc gtacaagaag
     8701 gcgcgcgtcg cccaggcgaa ggcctacgcg cagggctgcg ccaagcggtc gggcagcggg
     8761 ctgaagtact tcaactccct caacaacgcg cgtgacctgg acgtcatcag ggcagcgctg
     8821 ggcgagccga aactgacgtt catgggggcg tcgtacggca cctacttcgg ggcgctctac
     8881 gccaccctgt tcccctcgca cgtacggcgg atggtgttcg actcggcggt ggacccggcg
     8941 cccgagcaga tctggtaccg caacaacctg gagcagtcgg cggcgttcga gagccgctgg
     9001 acggacttcc gcaagtggat cgccaagcac gacgacgtgt acggactggg ccggacggtg
     9061 gccgcggtcc agcacagcta cgccaaggcg agcgcccggc tcgcggcgga gcccgcgggc
     9121 ggcaaggtgg ggcccgcgga gctgcaggcg gcgttcctgt cggccgggta ctacgacgac
     9181 tactggccga cgcgtgccca ggcgctgtcg gcgtatctga agggcgaccc gaagccgctg
     9241 atcgagcagg cggggcctgc gacggaggcg gcggccgagg cggagaactc caacgcggtg
     9301 tacacggccg tcgagtgcaa cgacgcgccc tggccgacgg acttcacggt ctgggaccgg
     9361 gacaacaccc ggctctcgcg ggtggcgccg ttcgagacct gggacaacgt gtggatgaac
     9421 ctgccgtgtg cctactggcc gacgtcccgt cagcagccgc tggatgtgcg gaccgggccc
     9481 ggtgagctgc cgccgacgct gatccttgcc gccgagcggg acgcggcgac gccgtacgag
     9541 ggcgcccagg agatgcaccg gcggctgtcc ggctcggtgc tggtgaccga gcgggacgcg
     9601 ggcacgcacg ggatcgccgg cggcccgaac aagtgcgtca acggctacct cgacgcctac
     9661 ctgctggagg gccgcacacc cgacgagcag gcgtcctgcg ccccgcacgc ggagcccaaa
     9721 ccgaagccgc aggccgaccg cgcccgccag accccgctga aggagcccct gaagggcagc
     9781 accgcccgcc cctgacgacc ggcccccacc gccccggccg cccccctcga caaccaccac
     9841 aggccaccga ccccggcggc ccgtgcctct tcccagaggc cgcccaccaa gcacggccac
     9901 cgcccgaacg agcccctcgc ccagctcacc catcgaccta ccgccgcggc tcaggagggg
     9961 acggaccccc ggccgcacgc agagcaccgc cggtcggacg agcccctcga ccagcccgcc
    10021 caccgaccta ccccgcgccg ccccagaggg gacggacctc cggccattcg cagcaccgct
    10081 ggtcacaccc ctcgacaagc tcgctcaccg acttacggcg cccggtgcca cacctcccgg
    10141 cgaacaacgc accggccccc aggccacgcg caaagcaccg ccggtcggac gagccccgag
    10201 accagtcccc cgccgaccta ccgctgcgcc gcccgacccg gagtgaaccc cggccgcacg
    10261 cagagcaccg ccggtcggac gggccctcga ccagcccgtc caccgacctg ccgccgcgcg
    10321 gccctccccc ggagggaacc ccggccacgc gtaaagcacc gccgggtcag gcccatccct
    10381 cgaccagctc ggccaccgac ttgcggcgcc cggtgccgta ccttccggag gacaaccccc
    10441 ggcccccagg ccacgcgcaa agcaccgccg gtcggacgag ccccgagacc agctcccccg
    10501 ccgacctacc gctgcgccgc cccccggagg gaacccccga ccgcccgcaa agcaccgccg
    10561 gtcagaccca cccctcgacc agctcggcca ccgacttgcg gcgccccgtg tagaacggga
    10621 tctccgtgcg gacgtgcctg cgggcctcgg aggcgcggag gtggcgcatg aggtcgacga
    10681 tgcggtggag ttcgtcggcc tcgaaggcga ggatccactc gtagtcgccg agggagaacg
    10741 aggcgaccgt gttggcgcgc acgtccggga agccgcgggc catcttgccg tggtcggcga
    10801 gcatgcgccg gcggtcctcg tcgggcagca ggtaccactc gtaggagcgc acgaaggggt
    10861 acacgctgat gtagttgcgc ggcgtctcgt cggcgaggaa cgccgggatg tgcgcgcggt
    10921 tgaactcggc ggggcggtgc agcgccatgt tcgaccagac gggctccagg gcgcggccca
    10981 gcttcgtgcg gcggaagagg ttgtacgcct cctgcagctg ctcgctggtc tccgcgtgcc
    11041 accagatcat gaggtcggcg tcggcgcgca gaccggacag gtcgtacgta ccgcggatcg
    11101 tcacgtcctt ggcggcgagc tggtcgaaca gctcctggac ctcgtcggcg tagccggtgc
    11161 ggtcctcggg gagcgcgtcc ttcagcttga agacggacca cagggtgtag cggatgacct
    11221 cgttgaggtc cttggccagc ttgcccttgt tggggatcct cgtggcctcg gtggtgggaa
    11281 cgtcgtcact catgccctta ttctcccg
//
