LOCUS       NZ_CP028834            10882 bp    DNA     linear   CON 25-MAY-2024
DEFINITION  Streptomyces sp. M2 chromosome, complete genome.
ACCESSION   NZ_CP028834
VERSION     NZ_CP028834.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M2
  ORGANISM  Streptomyces sp. M2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8718751)
  AUTHORS   Worsley,S.F., Murrell,J.C. and Hutchings,M.I.
  TITLE     The chemical ecology of Streptomyces-plant root interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8718751)
  AUTHORS   Worsley,S.F., Murrell,J.C. and Hutchings,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2018) Biological Sciences, University of East
            Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom
            (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.99x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004104505.1-RS_2024_05_25
            Annotation Date                   :: 05/25/2024 03:18:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,020
            CDSs (total)                      :: 7,927
            Genes (coding)                    :: 7,816
            CDSs (with protein)               :: 7,816
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 39 of 111
            Pseudo Genes (incomplete)         :: 91 of 111
            Pseudo Genes (internal stop)      :: 7 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:04:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1943730
            Orig. end                         :: 1954612
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028834.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Matthew Hutchings,
            School of Biological Sciences, University of East Anglia, Norwich
            Research Park, Norwich, NR4 7TJ.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10882
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="10"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10882
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10882
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5882](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5882)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(80..100)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.39"
                     /tool="antismash"
     gene            complement(119..991)
                     /locus_tag="DBP13_RS08985"
     CDS             complement(119..991)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /GO_process="GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071440.1"
                     /locus_tag="DBP13_RS08985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="WP_241662292.1"
                     /transl_table=11
                     /translation="MLRIGVAPHRLWPAREDETDEVLATARRAEELGFDHVFVSGHAIA
                     GDQGVTPDPLVTLSAVAGATERIGLVTSVLVLPLYNPVVLAHQTATLDRLSRGRFTLGI
                     GTGWDTEEYAAVGVPFAGRGRRADEQLAAVRELWRGEGDVRLGVLPRTEGGPDVWVGGQ
                     SDTALRRALRYGEAWHGSGIDAAGLTAVRERLTGLGERAGRDPRTLALSAGAFLVPPGF
                     APAVQLPWRPLGGRDASADSVREELAALADAGLSSCSLWLPVAADALPEALVWTAEEIL
                     SSFHKVRED"
     misc_feature    1153..1168
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.45"
                     /tool="antismash"
     gene            1157..2419
                     /locus_tag="DBP13_RS08990"
     CDS             1157..2419
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431984.1"
                     /locus_tag="DBP13_RS08990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_128801637.1"
                     /sec_met_domain="p450 (E-value: 8.9e-18, bitscore: 63.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVRDAPQVPDVFDPRQYAAGVPHAAYRVLRDHHPVVWQDEPEVL
                     GWPAGPGFWAVSRHADVVRVLKDSATYSSHLGATQIRDPDPDDLPFIRRMMLNQDPPQH
                     GRLRRLVSRAFTPGRIERFTSVARERARGLLAQALRDARTQDGTVDLVAAVTDDYALLN
                     LADLLGVPDSDRGLLLHWTQRVIGYQDPDEAGAPVLDAAGKPVNPRSPAMLRDMFDYAQ
                     RLAAHKRRHPADDVLTTLAHEAELADAELEMFFFLLTVAGNDTVRSAAPGGLLALAEHP
                     EAYSSLRAGKCELSTAVDELLRWHPPVLTFRRTATEDTELAGRPVRAGDKVVVFHASAN
                     RDERVFAAPDRLDLSRAPNPHVSFGDGPHVCLGAHFARLQLRVLYEEVLRALLVLRTAG
                     AAVRLTSNFINGIKCLPVEVT"
     gene            complement(2458..3627)
                     /locus_tag="DBP13_RS08995"
     CDS             complement(2458..3627)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431985.1"
                     /locus_tag="DBP13_RS08995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="damage-control phosphatase ARMT1 family protein"
                     /protein_id="WP_128801638.1"
                     /transl_table=11
                     /translation="MSDVPSAPVILGDEPGSFPHGVLAERHPAIIRQVREAFPYGPAQH
                     RALDALLASCTEGVVEPLPAHAHDRARWEEWGAVGNTGRSWFKIPWLWSESYFYRQLLE
                     AVGYFGTGPWQGIDPFRPFKLAELDSPETDEELAALDALSAGDQDRALLHGSLWGNRAD
                     LGFRLSAGDAGPADAAPGLVADDSAALWSLLPQSGAGTLCLIADNAGRELIPDLLLIAH
                     LLERGRIARAVLHVKPYPYYVSDATTADVLDALRRLTGAEGAAVEYGSILWSAMTDGRL
                     VLRAHPFSSAPLPYADMPGDLRAELAAATVTIVKGDLNYRRLVGDRLWPPTTPFADVTA
                     HFPGPVAALRTLKSDVITGLDPATEAALVAAEGQSWRTGGTHALIQVRA"
     misc_feature    4160..4173
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.84"
                     /tool="antismash"
     gene            complement(4284..4931)
                     /locus_tag="DBP13_RS09000"
     CDS             complement(4284..4931)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431986.1"
                     /locus_tag="DBP13_RS09000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_128801639.1"
                     /transl_table=11
                     /translation="MARQLRAEQTRTTIIAAAADLFDRRGYESTSLSDIVEHAHVTKGA
                     LYFHFAAKEDLAHAIMELQSRALRDLTAEIDARGHSSLEALMRITFGIARLSVEGPIPR
                     AGLRLATGGVAVRPPAMHPFTEFRDMATRKLLGAVKESDLHPDIDVDAIAHSLVSFYVG
                     TRIVSRSLEPVTRQPRRLAEMWYVLIRGLVPVTRRARYLNLAARLEREIRPA"
     misc_feature    complement(4917..4919)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5001..5882)
                     /locus_tag="DBP13_RS09005"
     CDS             complement(5001..5882)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431987.1"
                     /locus_tag="DBP13_RS09005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_108006854.1"
                     /sec_met_domain="AfsA (E-value: 6.3e-24, bitscore: 82.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQPTLTATVPRQLVHRAAVAETYLTGWEKTGTDRFRLSAQWPRA
                     HQLHVSPDRSAHEPLLVAETVRQCGALLSHTAYEAPLDHCFVLQELRLTTHPEHLAVGA
                     APAEPVLDVRVFDIRRRSGRPTAFRYDTDVRLGGERVASAHIWVSWTSPSVYRRLRGGR
                     TVDTVSTLPPPPALPADAVGRALAADVVLAPPERPGRWQLRVDTAHPVFFDHPLDHVPG
                     MLLLEAARQAARATTGGPGSSASFRATFHRYAELDIPTWIEVMDADRKGLRVRGTQGGS
                     TVFECAVDAAAR"
     misc_feature    complement(5739..5741)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5913..5928)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.56"
                     /tool="antismash"
     gene            6152..7099
                     /locus_tag="DBP13_RS09010"
     CDS             6152..7099
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431988.1"
                     /locus_tag="DBP13_RS09010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="WP_128801640.1"
                     /transl_table=11
                     /translation="MSRMTAHRTALTAALVTPFLLPLWAAGPAQAHGAPVDPVSRVYAC
                     SPDGGSSRTAACRAAVAANGAPFTMWDNLRVPDVGGRDRQVIPDGKLCSGGLPAYKGLD
                     LARSDWPSTTLTPGATMTMKYASTIPHTGTFKLYLTKQGYDPTKPLTWSELPEQPFAAV
                     KDPALTNGAYRITAKLPSDRSGHHVLYTIWQNSSTTDTYYSCSDVVFRSASGSGGGGGA
                     AGSSAGGAKGSGGKKAASGSTSRTPGSSTSPATPPSHTAQAAPQQKTGAPDSTPVASAS
                     TADSGPSAPMLAGGAAAVLVLTGGAALALRLRRR"
     gene            complement(7118..7771)
                     /locus_tag="DBP13_RS09015"
     CDS             complement(7118..7771)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431989.1"
                     /locus_tag="DBP13_RS09015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Tat pathway signal sequence domain protein"
                     /protein_id="WP_108006858.1"
                     /transl_table=11
                     /translation="MRTRTLATLIGAVAALSIPAVVPASAADTPVLTTGSAGGTAVAVG
                     DVLTAPLASGTNATLYSSATGTSGVTCSSSTFTASVTDNPAAPGTATESVSGQSFDSSS
                     CTSNVLGVTGVTSITVDNLPYTTTVTSDGALSVTPASGSTIQTTVKLKTLLGSITCVYQ
                     APSLTGTASNDDSSIAFTNQEFTKTSGSSLCFSSGFFSAKYAPVTDNGATVYVN"
     misc_feature    8195..8210
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.38"
                     /tool="antismash"
     misc_feature    complement(8201..8214)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.6"
                     /tool="antismash"
     misc_feature    8227..8247
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.6"
                     /tool="antismash"
     gene            8391..9059
                     /locus_tag="DBP13_RS09020"
     CDS             8391..9059
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996942.1"
                     /locus_tag="DBP13_RS09020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6230 family protein"
                     /protein_id="WP_108006860.1"
                     /transl_table=11
                     /translation="MASSSDVTSADHTPGNPESGSATDPARRGRVRLRRAAVMAVPAVA
                     VAAGLAIMTAEGALGVSFSISGMPFTVTAKSLDGTGFEQFGGLDSMIDNSPNQGDTGGQ
                     VLIVTSAIKNATLDSLCQSVDLGGTNLLIKAGSGSSKVKATDLTTDSTQLSGDASFNNI
                     EIGNDASTLDKAGVKGPKGVFSQQADTVHIANLRQTNYATTAGVFKLPGLKLSFSDSGC
                     "
     gene            9059..9496
                     /locus_tag="DBP13_RS09025"
     CDS             9059..9496
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431991.1"
                     /locus_tag="DBP13_RS09025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6114 domain-containing protein"
                     /protein_id="WP_108006862.1"
                     /transl_table=11
                     /translation="MPKRWEQGVRPAFRQWRARRPFWGGLLLALGGAEILLTEKASLKV
                     VMHIGMQGLAGYLLPVVMVLCGLLTLFSPSQRLFYSIIGVLASLGSWITSNLGGFFIGL
                     LLGAVGSCLVFGWLPDQEPRRSRRRRRREARTQHEEQGVPA"
     gene            9633..10457
                     /locus_tag="DBP13_RS09030"
     CDS             9633..10457
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1292: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921432.1"
                     /locus_tag="DBP13_RS09030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_128801641.1"
                     /transl_table=11
                     /translation="MPEDSARPGDRTGIDTGTAHAARIYDYILGGKDHYPADREAGDAM
                     CREWPALPVHMRANRDWLNRAVRRLAEEAGMRQFLDIGTGIPTSPNIHEIAQSAAPGSR
                     VVYVDNDPLVLTLSQSLLNSTPEGRTSYVEADMRDPRTILDSPAFRDTLDPAEPVALTV
                     VAIVHFMLDEDDAVGIVRRLLEPLAPGSHLAMSIGTADFAPREVGRVAREYAARGMPMR
                     LRTFAEAQEFVEGLELVEPGLVQVHKWRPDGTDTGAIRDEDIAMYGVVARKP"
ORIGIN
        1 attcccggca tcgatcgacg taacgccatg cgggcatgac taccagggat ttgtcatgat
       61 caccacatgt cgatcggggg aatcgtcaaa ccgtcatcaa tccgtcgctg gcccgtcgtc
      121 agtcctcacg gaccttgtgg aaggacgaga ggatctcctc agccgtccat acgagcgcct
      181 ccggcagggc gtcggccgcc accggcagcc acaacgagca ggaggacagc ccggcgtccg
      241 ccagggcggc cagctcctcc cgcacgctgt cggcgctcgc gtcccgtccg cccagaggcc
      301 gccacggcag ctgtacggcc ggcgcgaaac ccggcggaac gaggaaggca cccgcgctca
      361 gcgcgagcgt gcgcggatcg cgcccggccc gctccccgag accggtcagc cgttcccgca
      421 ccgccgtgag cccggcggcg tcgatccccg agccgtgcca agcctcgccg taccgcagcg
      481 ccctgcgcag cgccgtgtcg ctctgcccgc cgacccatac gtccggaccg ccctcggtgc
      541 gcggcagcac cccgagccgt acgtcgccct cgccgcgcca caactcccgt acggcggcca
      601 gctgttcgtc ggcgcgacga ccgcggcccg cgaaggggac gccgacggcc gcgtactcct
      661 cggtgtccca gccggtgccg atccccaggg tgaagcggcc gcgagagagg cggtcgaggg
      721 tggcggtctg gtgggcgagg acgaccgggt tgtacagcgg caggacgagc acgctggtca
      781 ccagcccgat ccgctcggtc gcaccggcca cggcggacag cgtcaccagc gggtcggggg
      841 tcacgccctg atcccccgcg atcgcgtggc cgctgacgaa gacatggtcg aagcccagct
      901 cctcagccct gcgcgcggtc gccaggactt cgtcggtctc gtcctcccgt gcgggccaca
      961 gacggtgcgg tgccactccg atgcgcaaca tgtcgtcacg ttcccctcgt ccggcagtgc
     1021 ggtcacggaa gcgtccggca gtgctgtcag gaagcctccg gcagtgcggt cgaggaaggc
     1081 aacaccgcgg ccggtgcggg catgcccgcc gcgcgctgtc ttgcccgggg cccgtccccc
     1141 gcgtcaccgt ggtcgcatga atgtgcgcga cgccccgcag gtcccggacg tcttcgaccc
     1201 gcggcagtac gccgccgggg tgccccatgc cgcctatcgg gtcctgcgcg accaccaccc
     1261 cgtggtctgg caggacgagc ccgaggtgct ggggtggccc gccgggcccg ggttctgggc
     1321 cgtgagccgg cacgcggacg tcgtacgggt cctcaaggac tcggcgacgt actcctccca
     1381 tctcggcgcg acccagatcc gcgatcccga cccggacgat ctgccgttca tccgccgcat
     1441 gatgctcaat caggacccgc cgcagcacgg gcggttgcgg cgtctggtca gccgcgcctt
     1501 caccccgggc cgtatcgagc gcttcacgtc ggtcgcgcgg gagcgggcgc ggggcctgct
     1561 cgcacaggcg ctgcgggacg cccgtaccca ggacggcacc gtcgacctcg tggcggccgt
     1621 gaccgacgac tacgccctgc tcaacctcgc cgacctgctc ggcgtcccgg acagcgaccg
     1681 cggcctgctg ctgcactgga cgcagcgggt catcggctac caggacccgg acgaggcggg
     1741 cgcacccgtg ctcgacgcgg cgggcaaacc ggtcaacccc cgttccccgg ccatgctccg
     1801 ggacatgttc gactacgccc agcgcctcgc cgcgcacaag cggcggcacc cggccgacga
     1861 cgtgctgacc acgctcgcgc acgaggccga actcgccgac gccgaactgg agatgttctt
     1921 cttcctgctc accgtcgccg gcaacgacac cgtgcgcagc gccgctcccg gggggctgct
     1981 cgcgctggcc gagcacccgg aggcgtactc gtcgctccgc gcggggaagt gcgaactctc
     2041 cacggcggtg gacgagttgc tgcgctggca cccgcccgtt ctcactttcc gccgcacggc
     2101 caccgaggac accgagctgg cggggcggcc cgtccgggcg ggcgacaagg tggtcgtctt
     2161 ccatgcctcc gccaaccgcg acgaacgcgt cttcgccgca ccggaccgac tcgacctctc
     2221 ccgcgcaccg aacccccacg tctccttcgg ggacggcccg cacgtctgcc tgggcgcgca
     2281 cttcgcacgt ctgcaattgc gggtgctgta cgaggaggtg ctgcgggccc tgcttgtgct
     2341 gcggacggcg ggggcggcgg ttcggctgac gtcgaacttc atcaatggca tcaagtgctt
     2401 gccggtggag gtgacttgag ggaggaggtg acttgaggga ggaggggggc tacgggctca
     2461 ggcgcggacc tggatcaggg cgtgtgtgcc gccggtccgc cagctctgcc cctccgccgc
     2521 gaccagagcg gcttccgtgg cggggtccag gccggtgatg acgtcggact tcagggtgcg
     2581 cagggcggcc accgggccgg ggaagtgggc cgtgacgtcc gcgaaggggg tggtcggggg
     2641 ccacagccga tcgcccacca ggcggcggta gttgaggtcg cccttcacga tggtcaccgt
     2701 ggcggcggcg agttcggcgc gcaggtcgcc aggcatgtcg gcgtacggca gcggggcgct
     2761 ggagaaggga tgggcgcgca gtacgaggcg gccgtcggtc atcgccgacc acaggatgct
     2821 gccgtactcg acggcggcgc cctcggcgcc ggtcagtctc ctcagcgcgt cgagcacgtc
     2881 ggcggtcgtg gcgtcggaga cgtagtacgg gtacggcttg acgtgcagga cggcacgggc
     2941 gatgcgcccg cgctccagga gatgggcgat gagcaggaga tcggggatga gttcgcgtcc
     3001 ggcgttgtcc gcgatcaggc agagggtgcc ggcgccggac tgcgggagca gtgaccagag
     3061 ggccgcgctg tcgtcggcga ccaggcccgg ggcggcgtcc gcggggcccg cgtccccggc
     3121 ggagaggcgg aagccgaggt cggcgcggtt gccccagagg gagccgtgca gcagggcccg
     3181 gtcctggtcg cccgcggaca gggcgtccaa cgcggccagt tcctcgtccg tctccggtga
     3241 gtcgagttcg gccagcttga aggggcggaa cgggtcgatg ccctgccagg ggccggtgcc
     3301 gaagtagccg acggcttcga gcagttggcg gtagaagtag ctctccgacc agagccaggg
     3361 gatcttgaac caggaacggc cggtgtttcc aacagccccc cactcctccc agcgcgcccg
     3421 gtcgtgcgcg tgagcgggga gcggttcgac gaccccctcg gtgcagctcg cgagcagcgc
     3481 gtcgagcgca cggtgctgcg cgggaccgta ggggaaggcc tcccgcacct ggcggatgat
     3541 cgcggggtgc cgctcggcaa gcacaccgtg cgggaaggac cccggctcat caccgaggat
     3601 cacaggcgca gacgggacat cggacatgcg cctcacccta gggggtgtcc tgcggatcgg
     3661 ctcgggggcg gggcgggggt ttgaccggcg cgcggtccat gccgtgtgaa ggtcggtccg
     3721 gtgccaaggc ctgtccgggg tacggggtcg ctccgtggtc cgggccgatc tgaggacagg
     3781 cggatccggg acgggggtcc tgcggcatcg cggctggcgg tgtcggagcc ggtccgaggc
     3841 tggggccggc ccgcagttga ggccggtgga ggcactgggc cgtgggatgt cgggcgcccg
     3901 ttcggtgccg agatgcgtcc ggggcggaga ttcaccggtg ccaagcctgt ccggtgcgga
     3961 ggccgctcgg gtgccgagac ccgtccagtc ccgcggcccg cccagccccg gggccactgc
     4021 tgcgccaagc ccggtccgtc gccgaggccg gtccggggca gagattccat ccggtgccaa
     4081 ggcctgccca acgccggggt cgttccggtg ccgaggccgt ccggcgtcgg gccggttcgg
     4141 ggtcgagatc cacgtggtgt cgaggccgat ccatggtcga gcccggccct cggtcgaccc
     4201 gagcccacag tcaaggccgg cccacggtcg agtccagccc acagcccagg cctctgccgg
     4261 gcccgtgtcg cgccggcccg ctctcaagca ggccggatct cccgttccag cctcgccgcg
     4321 aggttcagat agcgggcccg gcgtgtgacg gggaccaggc cgcggatcag gacgtaccac
     4381 atctcggcca gtctgcgggg ctggcgggtg accggctcga gcgatcggct gactatgcgg
     4441 gtgccgacgt agaagctgac gagggagtgg gcgatggcgt cgacgtcgat gtccgggtgg
     4501 aggtcggact ccttgacggc gccgaggagt ttgcgggtgg ccatgtcccg gaactcggtg
     4561 aaggggtgca tggccggtgg ccgtacggcg actcccccgg tggcgagccg gaggcctgcc
     4621 cgcgggatcg ggccttcgac ggacagccgg gcgatgccga aggtgatgcg catcagggcc
     4681 tccagggagg agtgaccccg tgcgtcgatc tcggccgtca ggtcccgcag ggccctcgac
     4741 tgcagctcca tgatcgcgtg ggccaggtcc tccttcgccg cgaagtggaa gtacagcgcg
     4801 cccttggtga cgtgggcgtg ctcgacgatg tcgctgaggc tggtcgattc gtagccgcgg
     4861 cggtcgaaca ggtctgctgc ggccgcgatg atcgtcgtgc gggtctgctc ggcgcgtaac
     4921 tgcctcgcca tcggctgaac actcctggga ctggaacgaa cgggtcgtgc tgtttgtttt
     4981 tactccccgt gaacgatatc tcaccgtgcg gcggcgtcca cggcgcactc gaagaccgtc
     5041 gaaccgccct gcgttccccg gacccggagc cccttgcggt ctgcgtccat gacctcgatc
     5101 caggtgggta tgtccagctc ggcgtatcga tggaaggtgg cgcggaagga cgcggacgag
     5161 cccggcccgc cggtggtcgc gcgggccgcc tgccgtgccg cctccagcag gagcatgccc
     5221 ggtacgtgat cgaggggatg gtcgaagaaa acgggatgcg cggtatctac ccgcagttgc
     5281 cagcgccccg gacgctcggg cggcgcgagc acgacgtccg ccgcgagcgc acggcccacc
     5341 gcgtcggcag gcagcgcggg cggtggcggc agcgtcgaga cggtgtccac ggtgcggccg
     5401 ccgcgcagcc tgcgatagac ggaggggctg gtccagctca cccagatgtg cgcgctcgcg
     5461 acccgctccc cgcccaggcg cacgtcggtg tcgtagcgga acgcggtcgg gcggccggag
     5521 cgccggcgga tgtcgaacac cctgacgtcc aggacgggtt cggcgggtgc ggcgcccacg
     5581 gcgaggtgct cggggtgtgt ggtcaggcgc agttcctgca ggacgaagca gtggtcgagc
     5641 ggggcctcgt acgcggtgtg ggacagcagc gccccgcact ggcgcacggt ctccgcgacg
     5701 agcagcggct cgtgggcgga gcggtccggg gacacgtgta actgatgggc ccgtggccac
     5761 tgcgcactca gcctgaaccg gtccgtcccg gtcttctccc agccggtgag atacgtttcc
     5821 gcgaccgcag cccggtgcac gagctgacgg ggaacggtgg ccgtcagggt cggttgaggc
     5881 atgtccctcc ctcggtcccc cgaaagcgat gacagaccag acggacaggg ccgtaccggc
     5941 gaccgccaga gtacgaggcg accggttggt tttcaatgag acggacgtgc ctcaaggggc
     6001 ggacggcgca ggtgaacggg cggcgccggg cgcacgttcg gataccgggc gccctctttc
     6061 actccggggc tcacacggtg aacgcgtcgc acccgtcgtc cgtactcacc gggagcacac
     6121 cgatcccgcg ccggccgagg agacgttccg gatgtcccgg atgaccgcac accgcaccgc
     6181 cctgacggcc gccctcgtga ccccgttcct gctccccctg tgggcggcgg gacccgcgca
     6241 ggcgcacggc gcacccgtcg atccggtcag ccgggtctac gcctgctccc ccgacggcgg
     6301 cagttcccgc acggcggcct gcagggcggc cgtcgccgcg aacggcgccc ccttcacgat
     6361 gtgggacaac ctgcgggtgc cggacgtggg cggcagggac cggcaggtca ttccggacgg
     6421 caagctgtgc agtggcggcc tgcccgccta caagggcctg gacctggcgc gctccgactg
     6481 gccgtcgacg acgctgacgc cgggcgcgac gatgacgatg aagtacgcct cgacgatccc
     6541 gcacaccggc acgttcaagc tctatctcac caagcagggc tacgacccca cgaaaccgct
     6601 cacctggtcc gagctgccgg agcagccgtt cgcggcggtc aaggacccgg cgctgacgaa
     6661 cggcgcgtac cgcatcacgg cgaagctgcc gtccgaccgg agcgggcacc acgtgctgta
     6721 caccatctgg cagaacagca gcacgacgga cacgtactac tcctgctcgg acgtcgtgtt
     6781 ccggtcggcg tccggcagcg gcggaggcgg cggtgcggcg gggtcgtcgg ccgggggcgc
     6841 gaaggggagc ggcggcaaga aggccgcgag cggcagcact tcgcggacgc cggggtcctc
     6901 cacgagtccc gccacgcccc cttcgcacac cgcgcaggcg gcgccgcagc agaagaccgg
     6961 cgcccccgac agcactccgg tggcctccgc ctccaccgcg gactccggtc cgtccgcgcc
     7021 catgcttgcg ggcggcgccg cagcggtcct ggtgctcacc ggcggcgccg ccctggcgct
     7081 gcgtctgcgt cgacgctgaa gtcgccctgc gcgatggtca gttgacgtag accgtcgcgc
     7141 cgttgtcggt caccggggcg tacttggcgc tgaagaagcc gctgctgaag cagagcgagg
     7201 agcccgacgt cttggtgaac tcctggttgg tgaaggcgat gctgctgtcg tcgttgctgg
     7261 ccgtgccggt caggctcggg gcctggtaga cgcaggtgat gctgcccagc agcgtcttca
     7321 gcttgaccgt ggtctggatg gtggacccgc tcgccggggt caccgagagg gcgccgtcgg
     7381 aggtcacggt ggtggtgtac ggcaggttgt cgacggtgat gctggtgacg ccggtgacgc
     7441 ccaggacgtt gctggtgcag ctgctgctgt cgaagctctg cccgctgacc gactcggtgg
     7501 cggtgcccgg cgcggcgggg ttgtcggtga cgctcgcggt gaacgtcgag gacgagcagg
     7561 tgacgccgct cgtgccggtc gcgctggagt agagggtggc gttggtgccg gaggccagcg
     7621 gtgcggtgag gacgtcaccg accgcgaccg cggtgccgcc ggccgaaccg gtggtcagga
     7681 cgggggtgtc ggccgcggag gcggggacga cggcggggat ggagagggcg gcgacggcgc
     7741 cgatgagggt ggcgagggtg cgagtacgca tgcgggggcc tcttctctgg gatgtggggg
     7801 ctgaaggtgg cgtgggggga cgctggactg cgtccgccgc caccggtccg tgacgccttc
     7861 tccgtacgtg acggaccggc aacggcgacc ggccggtgac cggcgaggga cccgtggaaa
     7921 gggccatgtg ccgggaccgg gcggggaagc cggcccgcac gggacagggg gaagtacccg
     7981 tgcggagccg tcgagagcgt gggggtgtgt gggattccgg gagcgcgggt catgccgcgc
     8041 gacggggatc cggcgccacg gatcctgggg gaagccaggg gcagttgtag acctgagact
     8101 cagtcaacgt caaggctgcg tgcggcagtt gacgatcgtt caagtgttgt attggggctt
     8161 cgtcgcagga cggaggcaca ggtgaggcac aacggacact cttttttcac aactcccttg
     8221 acccttaaat gtaaccacgg gtaacttcct gattcggcta ctgcggagta cgtgaaagcc
     8281 cttgcgcccg ccgggagttg cgaggaggcg tgcaccgctg tccgctgtcc gcgccaggac
     8341 aacgtgccgc tgatgcccaa cccgcaatga ccacgcacat gggagcagaa atggcctcgt
     8401 cctcggacgt cacgtccgcc gaccacaccc cagggaaccc ggaaagcggt tccgcgaccg
     8461 accccgcgag acgcgggcgg gtccggctgc gccgtgccgc cgtgatggcc gtgcccgccg
     8521 tcgcggtcgc cgccggactg gcgatcatga ccgcggaggg agcgctgggg gtgtcgttct
     8581 cgatatccgg catgcccttc acggtcaccg ccaagtcgct ggacggcacc ggattcgagc
     8641 agttcggcgg gctcgacagc atgatcgaca acagcccgaa ccagggcgac accggcggtc
     8701 aggtgctgat cgtcacctcc gcgatcaaga acgccacgct ggacagcctg tgccagagcg
     8761 tggacctggg cggcaccaac ctgctcatca aggcgggcag cggctcgtcg aaggtgaagg
     8821 cgaccgacct gaccaccgac tccacccagc tgtccggtga cgcctcgttc aacaacatcg
     8881 agatcggcaa cgacgcgagc acgctcgaca aggccggcgt caagggcccg aagggcgtct
     8941 tcagccagca ggccgacacg gtgcacatcg ccaacctgcg gcagacgaac tacgcgacga
     9001 ctgcgggcgt cttcaagctg ccgggtctga agctgtcgtt cagcgactcg ggctgctgat
     9061 gccgaagcgc tgggaacaag gcgtgcggcc cgcttttcgt cagtggcggg cccgccgtcc
     9121 gttctggggc gggctgctgc tcgctctggg cggggcggag atcctgctca ccgagaaggc
     9181 ctcgctgaag gtcgtcatgc acatcggcat gcagggcctg gccgggtatc tgctgcccgt
     9241 ggtcatggtg ctgtgcggtc tgctgaccct cttcagcccc tcgcagcggc tcttctactc
     9301 catcatcggc gtcctggcct ccctgggttc ctggatcacc tccaacctgg gcggcttctt
     9361 catcggcctg ctcctcggcg ccgtgggcag ctgtctcgtc ttcggctggc tgccggacca
     9421 ggagccgcgc cgcagccggc gccggcgcag gagggaagcg cggacacagc acgaggagca
     9481 gggcgtgccc gcctgaccgg ggagcgggca cggccccggc cgcagccccc gtcatgtgac
     9541 tcccgtggcc caagtggttt gccccgcccg ccggttgacg aagtagaggg cggggccgcg
     9601 tgcggcggga cgacgtgacg agaggggcca tcgtgcccga ggacagcgcc cggccgggcg
     9661 accggaccgg catcgacacc ggcaccgcgc atgccgcgcg gatctacgac tacatcctgg
     9721 gcggcaagga ccactacccc gccgaccggg aggcgggcga cgccatgtgc cgggagtggc
     9781 ccgccctgcc ggtccacatg cgggccaacc gcgactggct gaaccgcgcg gtgcgccggc
     9841 tggccgagga ggcggggatg cgccagttcc tcgacatcgg caccggcatc cccacctcgc
     9901 ccaacatcca cgagatcgcc cagtcggcgg cccccggatc acgggtggtc tacgtcgaca
     9961 acgacccgct cgtcctcacg ctctcgcaga gcctgctgaa cagcacgccc gagggccgca
    10021 cctcctatgt cgaggcggac atgcgcgatc cgcggaccat cctcgacagc cccgccttcc
    10081 gcgacaccct ggacccggcc gagccggtcg cgctcaccgt ggtcgcgatc gtccacttca
    10141 tgctcgacga ggacgacgcc gtgggcatcg tccgccggct gctcgaaccg ctcgcgccgg
    10201 gcagccatct cgcgatgtcc atcggcaccg ccgacttcgc gccgcgggag gtcggccggg
    10261 tggcccgcga gtacgcggcc cgcggcatgc cgatgcggct gcgcaccttc gccgaggcgc
    10321 aggagttcgt cgagggcctg gagctcgtcg agccgggact cgtccaggtc cacaagtggc
    10381 gtccggacgg tacggacacc ggggcgatcc gcgacgagga catcgcgatg tacggcgtgg
    10441 tggcccgcaa accgtagccg cacaccctgc tgcccggcgt gcatcatcac gatgcggcag
    10501 cgacaactca ctgaatgttg ctgatcactt gactaccggc ggtcccacgc gattggctcc
    10561 ggccagcgag agtcaccctt gcgtcgtccg acgtcggacg acggacggaa gccctctctc
    10621 gccgttccac ttgctcggcc gcacagcgag gtgccgtccc ctcatgaata ctcctccccc
    10681 accgtccgcc gcccacagat ccgcccgtgt gatcgccgcg gccgccgccg tctgcctgtt
    10741 cgcggccggg tgctccgggt ccaaggacgg ctccgactcc gctcccggcg ccggcggcag
    10801 ggcggacacc gcccgcctca aggtcgccct ggtcacccat gcctcggagg gcgacacctt
    10861 ctggaacctg gtgaagaagg gc
//
