LOCUS       NZ_CP028834            10813 bp    DNA     linear   CON 25-MAY-2024
DEFINITION  Streptomyces sp. M2 chromosome, complete genome.
ACCESSION   NZ_CP028834
VERSION     NZ_CP028834.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. M2
  ORGANISM  Streptomyces sp. M2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8718751)
  AUTHORS   Worsley,S.F., Murrell,J.C. and Hutchings,M.I.
  TITLE     The chemical ecology of Streptomyces-plant root interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8718751)
  AUTHORS   Worsley,S.F., Murrell,J.C. and Hutchings,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2018) Biological Sciences, University of East
            Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom
            (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.99x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004104505.1-RS_2024_05_25
            Annotation Date                   :: 05/25/2024 03:18:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,020
            CDSs (total)                      :: 7,927
            Genes (coding)                    :: 7,816
            CDSs (with protein)               :: 7,816
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 39 of 111
            Pseudo Genes (incomplete)         :: 91 of 111
            Pseudo Genes (internal stop)      :: 7 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:04:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 31223
            Orig. end                         :: 42036
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP028834.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Matthew Hutchings,
            School of Biological Sciences, University of East Anglia, Norwich
            Research Park, Norwich, NR4 7TJ.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10813
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10813
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10813
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5813](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5813)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    413..429
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.7"
                     /tool="antismash"
     misc_feature    1550..1564
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.37"
                     /tool="antismash"
     gene            complement(1960..2622)
                     /locus_tag="DBP13_RS00180"
     CDS             complement(1960..2622)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430197.1"
                     /locus_tag="DBP13_RS00180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_234548399.1"
                     /transl_table=11
                     /translation="MAEQAPDKNSSRLTERGRATRERILRAAGDMMYVKGVSLTTLDDV
                     RAASGTSKSQLYRHYPDKDALVRDVVALQAAEVLDRHQQLLRRLNSFRGLERWRDAVVE
                     RNALRNGAYGCPLGSLASELADQDEEARKAIAQHFATWEGLLEAGLVRMKDSGVLRADA
                     DAKELATGLMAALQGGYLLARTAQDVTPMRIALDMAIAHVRAYAVPAEDDDADTSEG"
     gene            complement(2778..3532)
                     /locus_tag="DBP13_RS00185"
                     /pseudo=""
     CDS             complement(2778..3532)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432625.1"
                     /locus_tag="DBP13_RS00185"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="HAD-IA family hydrolase"
                     /pseudo=""
                     /sec_met_domain="HAD_2 (E-value: 3.8e-05, bitscore: 22.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLGPPAAIRACLFDLDGVITDTTSLYAAAWKEAFDTLLQQHEGE
                     GFRPFAADTGYDEFFDGRSSAEGVRCFLSSRGIERPDGSPDDPPGPDTVYGLVRWKNSL
                     FEARTRSQGVQVYQDTMAYVMAARDSGLLTAVVASSANCREILRAARMETLFDAWIDGV
                     VAASRNLRGKPHPTHSWQLPAIWASTLHSARYSRTRRQAWTPDDRVTSGGWSASPGQSA
                     PGRCARMAPMWSSKTWET"
     gene            3952..4596
                     /locus_tag="DBP13_RS00190"
     CDS             3952..4596
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432626.1"
                     /locus_tag="DBP13_RS00190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RraA family protein"
                     /protein_id="WP_128800754.1"
                     /transl_table=11
                     /translation="MEDDALAARAARLGCATLVDAVGRVHGHRAHIPVMSSPDPLRPLS
                     DPRPPSPTCPFRDDLAGTVRDFKHYFYGALGQEPAAGQVLVLSSGGHPEVSHGGGTKLA
                     RADGADLAGVLADGRLRDFDQLRGTASPPGAPGEAVRWGGDTIMPFAYGVAVEICGVCV
                     NPGDYVHMDSAGGVVIPSATLHRVFAIAEDIAAEEAEAAGVIRGENAQPGG"
     gene            4676..4915
                     /locus_tag="DBP13_RS00195"
     CDS             4676..4915
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432627.1"
                     /locus_tag="DBP13_RS00195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_128800755.1"
                     /transl_table=11
                     /translation="MTQTSHRSSHIVVHTPALDGSRRVTEGDVILGVASHLDDVVEILR
                     LADLDQIDVEESEHIEWQGGGPDEWPGLTEHPQE"
     gene            complement(5001..5813)
                     /locus_tag="DBP13_RS00200"
     CDS             complement(5001..5813)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432628.1"
                     /locus_tag="DBP13_RS00200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_128800756.1"
                     /sec_met_domain="Linocin_M18 (E-value: 6.7e-92, bitscore:
                     306.0, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRDLAPLSAAAWSDLEEEARRTFTTHAAARRIVDVPEPGGV
                     ELGGVATGHLDSVETPADDVVAHVRRFQPLVELRVPFIVDRRQVDDVARGAKDTDWQPA
                     KDAARRLAHVEDRAVFEGYAAAGIAGLRESSSNSPVKLPADVRDYPDAISQAVSALRLA
                     GVDGPYCLALSAEAYTAVSETSAHGYPIIKHIARLVDGDIVWAPAIDGAFLLSTRGGDF
                     EMRLGQDVSIGYLSHDATTVTLYLEETLTFLVHTSEAVAALVSQPTGT"
     gene            complement(5810..6880)
                     /locus_tag="DBP13_RS00205"
     CDS             complement(5810..6880)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432629.1"
                     /locus_tag="DBP13_RS00205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_128800757.1"
                     /transl_table=11
                     /translation="MADETSAPTPQTVLCPLTSAAIFLVVTIDLGGEAVTRDVLSDLNS
                     LQRSVGFRAEPEGRLSCVTGIGSEAWDRLFAGPRPADLHPFRELNGRVHRAVSTPGDLL
                     FHIRAARLDLCFALASEIMSRLGKAVTVRDDVHGFQYFDKRDLLGFVDGTENPTGAAAR
                     AAVLIGDEDCEFAGGSYVIVQKYVHDLAAWNTLPVEAQERIVGRTKLSDIELDDSTKPA
                     DSHVALTTITGADGTEREILRDNMPFGTVGRGEFGTYFIGYARTPGVTEAMLERMFLGT
                     QDAAHDRILDFSTPVTGCLFHVPTADFLDDLPAPPGARSGAMGADVHAVVAKPESQPRS
                     SDTSLRIGSMKRSASR"
     gene            complement(7001..7330)
                     /locus_tag="DBP13_RS00210"
     CDS             complement(7001..7330)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432630.1"
                     /locus_tag="DBP13_RS00210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_108015999.1"
                     /transl_table=11
                     /translation="MNKKLNGVSAEDGAPVPAEEIPAALTAGRLVYTDGATQTFDSDGT
                     TRYVEGGRETEGTWFVDDTGHFCSFWPPSYRASYDLRWMVEGGEAVGLRFSDLRSGSHF
                     EGRYQ"
     gene            complement(7431..7901)
                     /gene="msrB"
                     /locus_tag="DBP13_RS00215"
     CDS             complement(7431..7901)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432631.1"
                     /locus_tag="DBP13_RS00215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_108016000.1"
                     /transl_table=11
                     /translation="MPEYTKSPERLAQLTEVQYRVTQQDETEPSFANALWDNKSPGIYV
                     DIVSGEPLFSSVDKYDSRTGWPSFTKPLEPGHVVERPDTSHGMVRVEVRSVNGDSHLGH
                     LFPDGPSDMGGLRYCINSASLRFIPVAELEKEGYGQYLSMFQMPDRSAAETE"
     gene            complement(8033..8533)
                     /gene="msrA"
                     /locus_tag="DBP13_RS00220"
     CDS             complement(8033..8533)
                     /EC_number="1.8.4.11"
                     /GO_function="GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_function="GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432633.1"
                     /locus_tag="DBP13_RS00220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="WP_108016001.1"
                     /transl_table=11
                     /translation="MAHEKAILAGGCFWGMQELFRALDGVVSTRVGYTGGETANPTYRN
                     HGSHAEAIEITYDPARTDYRALVEFFFQVHDPTTLNRQGNDVGLSYRSAIFSLGEEQRR
                     IAEATIAEVDASGLWPGKVVTEVVPAAAFWEAEPEHQDYLQRYPNGYTCHRIRPEWRLP
                     KAS"
     gene            complement(8801..8998)
                     /locus_tag="DBP13_RS00225"
     CDS             complement(8801..8998)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134894.1"
                     /locus_tag="DBP13_RS00225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_078516601.1"
                     /transl_table=11
                     /translation="MSEGTGDDEWGPHTPDAVLHTGTEELPDPEDVVLASGHDITPERL
                     EQARRLLEEEGAAGVEKLLP"
     gene            complement(9088..9270)
                     /locus_tag="DBP13_RS00230"
     CDS             complement(9088..9270)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DBP13_RS00230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_108016002.1"
                     /transl_table=11
                     /translation="MSSADSTAQQDAREALFEAIASQARQQAEHKDHGAVAAALKDLAE
                     AYAWAANQHESTGDQ"
     misc_feature    9342..9354
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.32"
                     /tool="antismash"
     misc_feature    complement(10503..10517)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
ORIGIN
        1 ggtccgcgac ctgcgatgcc gcgaacatgg ggtccgggca ggcgctcagg tcacggaaga
       61 gcgcggccgt ctccgtgtcg agcaggcggt acgagcgagt gagggacgcg cggacgtcga
      121 gcgcctggtc ctcctcgctc gcgaacgcat caagggttcg gtgttccggc gcctcgtact
      181 cgtcggccag gctcgccagg cgcagctgcg gtcggtaggc ggcgcgggcg gcgacgttgg
      241 ccagggccaa cgggagctgc ccggccagcc ggacgatgcg gtcgacggcc tctggttcgg
      301 ctgccgtcct cgccgcgccc agccggcggg ccagcaggct gcgtgcgtcc gcctggctca
      361 ggggcatgag cgtcagggga agcgcctggt aggccgcgac gagcgcgtgc agttgcatac
      421 gactggtgac gagggccatg gagccgggcc ctccggggag gaggtgtttg acctggtccg
      481 tgttccatac gttgtcgagc aggacgagca cgcgacggtt gcccagcagg ccgcggaaca
      541 tgagtcctcg tgcctgcgtc gtctcgggca tgtcctcggg gcgcactccc aaggactgca
      601 ggaagtcctc gagcacgtgc gcggggtccc gcggctcgac ctcgggcccg gctccctgca
      661 ggtcggcaaa gagctgtccg tccgggaagc ggtccgccag acggtgggcc acagtcgtcg
      721 cgaacgctgt cttgccgacg ccggccagtc cgccgatcgt gcagatggcc acggacgacg
      781 acgcttccgc gtcggcgacc aaccgctcga cctgctcctg ctccctctcg cggccgacga
      841 gactcggcag ggcggccggg agctgggccg gagcgtgagg cgagggtgcg cgcggcgcgc
      901 tcttcggtag gtcggtggat tgcgcgagct cgagtcgccc ggacaggatg cgctcgtgga
      961 cctgctgcag ttccggcgac gggctgacac cgaggcgttg acgcagcaga tgactggtcg
     1021 tgtggaagat cgtcagggcc tcggactgcc ggccggcacc gtagagcgcc gtcatgagga
     1081 ggccgcatac gcgctctcgt agcgggtgct cgacaagcag ggcggacagt tccgccgcgg
     1141 cttcggcgta gcgccctcgc tccacctcgc aggcgagctg ctcctcggca caggcgagac
     1201 gcagctcgcc gaggacgctg cgctgctggt ccgcgaacgg ccccgggaca ccggcgagag
     1261 ccactccctt ccacatctcg aggcccttgg tcagcaggct cgtcgcagac gccaggtcgc
     1321 cggtgctgcg cgcctctcgc gccttggccg cggtctcctt gaacctgttc aggtccagag
     1381 cgtccggttg gatgtgcagc cggtagcccc cgtcgaccag gtgtacggag ctgtcgctct
     1441 gctctcccag tgcctgtctg agccggtaga tgtaggtgcg cacggagcct atggccgacg
     1501 gtggggcttc gtccccccac acgtcctcca ccagtttcga gaggctgaca ggccggccgg
     1561 ccctgagcaa cagcgaagca aggactgcgc actgcctggg ctgaccagct ccggcctcga
     1621 ccccctgcct gtgtagccgg accgggccga gaactgagaa ctggactccc gcatacatcg
     1681 tgcctaccgc cctggggatc cttgtgaggc ttcgctgctc gaccgcccgc aagggaccgc
     1741 cgtcccccca acgtacccca ggttcgttca tttttcagga ctaaatagtc ctctacttgt
     1801 cgaagctgtg cgtctttcgt gcagagggcc gacgcctttc ggtgacggct gatgcgcctg
     1861 tgaaagatga gacatgcgga cgaacgcgtt cgccgatggc cgtcacaccc cgaaccccgc
     1921 cgggaggacg gcaaagtcca gcagatgaag ggactgcact cacccttcag acgtatccgc
     1981 atcgtcgtcc tcagcgggca cggcataagc cctgacgtgg gcgatcgcca tgtcgagggc
     2041 gatcctcatg ggtgtgacgt cctgagcggt ccgcgcgagg aggtacccgc cctgaagagc
     2101 tgccatgaga ccggtggcga gctctttggc gtccgcatcg gcgcgcagga ctcccgagtc
     2161 cttcatccgc accagtccgg cctcgaggag cccctcccag gtggcgaagt gctgggcgat
     2221 ggccttccgg gcctcttcgt cctgatcggc cagctcgctc gcgagggagc cgagagggca
     2281 gccgtacgcc ccgttgcgga gcgcgttgcg ctccaccacc gcatcccgcc agcgctccag
     2341 cccgcggaag gaattgagcc gtcgcaggag ctgctggtga cgatcgagca cttccgcggc
     2401 ctgcaacgcg acaacgtccc gcaccagcgc atccttgtcg gggtagtggc ggtagagctg
     2461 ggacttgctc gttccgctgg cagccctgac gtcatcgagg gtcgtcaggg agacgccctt
     2521 gacgtacatc atgtcgccgg cggccctcaa gatgcgctct cgagtcgccc gcccgcgctc
     2581 ggtcagccgg gagctgttct tgtcgggtgc ctgttccgcc acattggcca gattaccgcc
     2641 acggaccata gcatgttctg gaaaggacct caaggtcctt tttcgacccg cccagcgact
     2701 cggccgccga agacggtgcg agcactctct ccgtgcacat gactcacggt gcaacggttt
     2761 caggaggggg tggccgatca cggccgaggg cgcggatcag gcgtgccggt caggtctccc
     2821 aggtctttga cgaccacatc ggcgccatgc gcgcacaacg ccccggcgcg gactgtcctg
     2881 gcgacgccga ccacccaccc gaagtgaccc gatcgtccgg cgtccatgcc tgcctgcgcg
     2941 ttctcgaata ccgcgcactg tgcagggtgc tggcccagat cgcgggcagc tgccaggaat
     3001 gtgtcgggtg aggcttgccg cgcaggttcc gactcgcggc caccacgccg tcaatccacg
     3061 cgtcgaagag tgtctccata cgcgccgccc gcaggatctc gcggcagttg gcgcttgagg
     3121 cgaccacagc ggtgagcagc ccgctgtcac gggccgccat gacgtaggcc atggtgtcct
     3181 ggtacacctg gaccccctgt gaccgtgtcc tggcctcgaa caggctgttc ttccagcgga
     3241 ccagcccata caccgtgtcc gggcccggag ggtcgtcagg gctgccgtcg ggccgttcga
     3301 tcccgcgcga ggacaggaag caccgcacac cttcggcgct cgaccggccg tcgaagaact
     3361 cgtcgtatcc ggtgtccgcg gcaaacgggc gaaacccctc gccctcgtgc tgctgcagca
     3421 acgtgtcgaa cgcctccttc cacgcggcgg cgtacaggga ggtcgtgtcg gtgatcacgc
     3481 cgtcgaggtc gaagagacag gcccgtatcg cggcaggcgg accaagtgtc acagagtcgt
     3541 ccttctctca agtaccggta gggcggccag tgttgtccgc cgtgccgccg ccgcggaaca
     3601 cctgtagacg tgcggtggac gccggatcca cgaaccgacg tctacagctc cctctgttgg
     3661 ccgttgtcgg cgcctcacgc ctggcttggc tcgaccgatc gacgcgaagt agacgggccg
     3721 tagaggtact ggccactcac gccccagcgt cactgcggcc gatacggacc ggttctgaga
     3781 ccggtcggta agaacacgct gtagaagggc gctgacacac gtgccacacc ggcgccgggc
     3841 tgggtgctgt gcgctgcgag ccatgtgctc ggacttgttg gactgctgcg tcataggttc
     3901 gtgcccaact cccccaccgc tcggggccgg tggcccttcg aaaggaagcc gatggaggac
     3961 gacgccttgg ccgcaagggc tgcccgcctg gggtgtgcca cgctcgtcga cgccgtgggc
     4021 cgtgtccacg gccaccgggc ccacattccc gtcatgtcga gccccgaccc acttcgcccc
     4081 ctttcggacc cgcggccacc atctcctacc tgccctttcc gtgacgacct ggcgggaacg
     4141 gtccgcgact tcaagcacta cttctacggg gcactcgggc aagagcccgc cgcgggccaa
     4201 gtgctcgtgc tctccagcgg cggccatccc gaggtgtcgc acggcggggg caccaagctg
     4261 gccagggcgg acggcgccga cctcgccggc gtactggccg acggccggct gcgggacttc
     4321 gaccaactgc gcggcacagc ttcgccacct ggtgcaccgg gagaagctgt ccgctggggc
     4381 ggcgacacga tcatgccctt cgcgtacgga gtggccgtcg agatctgcgg cgtctgcgtg
     4441 aaccccggcg actacgtcca catggacagc gccggcggcg tcgtgatccc ttcagcgacc
     4501 ctgcatcggg tcttcgccat cgccgaggac atcgcggccg aggaggccga ggcagccgga
     4561 gtcatcaggg gcgagaacgc gcagccaggt ggatgaggtg cacgcagcgc cgcatgctcg
     4621 gcgggctgca caaggaggcc ccattccaat cgcatcagaa acaacgaggt tggtcatgac
     4681 acagacatcg caccggtctt cgcacatcgt ggtgcacacc ccggcgctgg acggctcccg
     4741 ccgggtcacc gagggtgacg tgatcctggg agtcgcctcc catctggacg acgtcgtcga
     4801 gatcctccga cttgccgacc tcgatcagat cgacgtcgag gagagtgaac acatcgagtg
     4861 gcagggaggt gggccggacg aatggccggg actcaccgaa cacccgcagg agtgaccgga
     4921 gcgctgccgg gctctgccat gtagtagccg ggccctggcg gtggttgtca ccggctgcgg
     4981 ccgaccggcg tttcaccccg tcatgtgccg gtcggctgcg acacgagggc tgcgaccgcc
     5041 tcgctcgtgt gcaccagaaa ggtgagggtc tcctccaagt acagggtgac agtggtcgca
     5101 tcgtgcgaga ggtagccgat cgacacgtcc tggccaaggc gcatctcgaa gtcgccgccg
     5161 cgagtcgaga gcaggaaggc gccgtcgatg gcgggcgccc agacgatgtc tccgtcgacc
     5221 aacctggcga tgtgcttgat gatcgggtat ccgtgggcgg aggtctcact gaccgccgtg
     5281 tacgcctcgg cgctcagcgc aagacagtac gggccgtcga cacctgccag tcgcagagcc
     5341 gagaccgcct ggctgatcgc gtccgggtag tcgcgcacgt cggcgggcag cttcaccggt
     5401 gagttggagg agctttcccg cagaccggcg atgcccgcgg ccgcgtaccc ttcaaagacg
     5461 gcccggtcct ccacgtgtgc gagtcgccgg gcagcgtcct tggcgggctg ccagtcagtg
     5521 tccttggccc ctcgcgcgac atcgtcgacc tgacggcggt cgacgatgaa cgggacacgt
     5581 agctcgacca acggctggaa acggcgcaca tgggcgacga cgtcatcggc aggggtctcc
     5641 acactgtcga ggtgccccgt cgccacacct cccaactcga ctccgcccgg ctcggggaca
     5701 tcgacgatgc gccgtgccgc agcgtgcgtt gtgaaggtgc ggcgagcctc ctcctccaga
     5761 tcgctccacg cggcggcgga gaggggagca aggtcacggt gcagattgtt catcgggacg
     5821 cgctccgctt catgctgccg atcctcaatg aggtgtcgga agaacggggt tgactctcgg
     5881 gttttgcgac gacggcgtgg acatcggcgc ccatggcccc cgatcgcgca ccgggtggtg
     5941 cgggcaggtc gtccagaaag tccgccgtgg ggacgtggaa gaggcatccc gtgacagggg
     6001 tggagaagtc cagaatccgg tcgtgtgcag cgtcctgcgt gcccaggaac atccgctcca
     6061 gcatcgcctc cgtcaccccc ggggtacggg catagccgat gaagtacgtg ccgaactcgc
     6121 cacgccccac ggttccgaag ggcatgttgt cccggaggat ctcgcgttct gtgccgtcgg
     6181 caccggtgat ggtggtgagg gcgacatgtg agtcggccgg cttggtgctg tcgtccagtt
     6241 cgatgtccga cagtttcgtc ctgccgacga tcctctcctg cgcctcgacg ggcaaggtgt
     6301 tccaggcggc caggtcgtgc acgtacttct gcacgatcac gtaactcccg ccggcgaact
     6361 cgcagtcctc gtccccgatc aggacggcgg ccctcgccgc cgcgcccgtc gggttctccg
     6421 tgccgtcgac gaagccgagc agatcacgct tgtcgaagta ctggaagcca tgcacgtcat
     6481 cgcgtacggt caccgctttc cccagccggc tcatgatctc cgaagcgagg gcaaagcaca
     6541 ggtcgaggcg tgcggcccgt atgtgaaaga gcaggtcgcc cggtgtcgac accgctcggt
     6601 gcacccggcc gttcagctca cggaacggat gcaggtccgc cggacgggga ccggcgaaca
     6661 accggtccca cgcctccgag ccgataccgg tgacgcagct gagcctgccc tcgggttcgg
     6721 cgcggaaacc cacagaccgt tgcagggagt tcaggtcgga cagtacgtca cgggtcaccg
     6781 cttccccgcc gaggtcaatc gtcacgacga ggaagatcgc cgcgctggtg agagggcaca
     6841 gaaccgtctg cggcgtcggt gccgacgtct catcggccat gtgccctccc tcttccgaca
     6901 gttgccgaca ctcgctgccg gccctgtgcg cggaccgtgt gcgcggccgc cacggactcc
     6961 ggtgaagatc ccgcacttcc tccagcaacg tctccaccgt tcactggtat cggccctcga
     7021 agtggcttcc gcttcgcaga tccgagaacc ggaggccgac agcctctccc ccctccacca
     7081 tccagcgcag gtcgtaggag gcccggtagg acggcggcca gaacgagcag aagtgcccgg
     7141 tgtcgtcgac gaaccacgtt ccctcggtct cccgtccgcc ctcgacgtag cgggtggtgc
     7201 cgtcggagtc gaaggtctgc gtcgctccgt cggtgtagac gagccggcct gcggtgagag
     7261 cggcgggaat ctcctcggcc ggtacaggtg cgccgtcttc ggctgaaacg ccgttaagtt
     7321 tcttgttcac tttggggacc tcccatgggc ccaggcgggc gttgcgcgag ctgtggcggt
     7381 gcgaggcggt ggtggccggg cagccattcg cggaccgcct gcccggccac tcactctgtt
     7441 tcggcggcgc tccggtccgg catctggaac atgctcaagt actggccgta gccctccttc
     7501 tcgagttccg cgacggggat gaagcgcaag gaggccgagt tgatgcagta ccggaggccg
     7561 cccatgtcac tggggccgtc agggaacaga tgcccgaggt ggctgtctcc gttcaccgac
     7621 cgcacttcga ctcggaccat tccatgactg gtgtcgggtc gttcgacgac gtgaccgggc
     7681 tccagaggtt tggtgaagct gggccatcct gtgcggctgt cgtacttgtc cacggaggag
     7741 aacaggggct ctcccgacac gatgtccaca tagatgccgg gagacttgtt gtcccagagg
     7801 gcgttggcga aggagggttc cgtctcgtcc tgctgggtta cgcggtactg gacttctgtg
     7861 agttgcgcga ggcgttcagg actcttggtg tattccggca tggctgcctc ccaagtggcg
     7921 tagctggatc agcccttctg ggatttcgcg tcatgtgccg ccgctccctt acttcgcggc
     7981 aagaaccgga gtggaacgac aaggttgccg ggatcagcag ccacgaggcc gactacgacg
     8041 ccttgggcag ccgccactcc gggcggatgc ggtggcaggt gtacccgttc ggatagcgct
     8101 gcaaatagtc ctgatgctca ggttcggcct cccagaaggc cgcggccggc accacctccg
     8161 tgacgacctt ccccggccac aggcccgaag cgtccacctc ggcgatcgtg gcctcggcga
     8221 tgcgccgctg ttcctcaccg agagagaaga tggcggaccg gtagctgaga ccgacgtcgt
     8281 tgccctgccg gttcagcgtg gtggggtcgt gcacctggaa gaagaactcg acgagagccc
     8341 ggtagtcggt ccgggcgggg tcgtaggtga tttcgatggc ctccgcgtgg cttccgtggt
     8401 tgcggtaggt cgggttcgcc gtttcgcccc ccgtatagcc gacgcgcgtc gagaccacgc
     8461 cgtccagggc ccgaaagagc tcctgcatgc cccagaagca tccgcccgcc agaatcgcct
     8521 tctcgtgtgc cattgccgtg ccgccctcct gtgaattccg ccgtctcgag ccgacgggtc
     8581 agagcacttg gatcgtgctc ttcgaccctg gccgtggtcc gttcggcacg gtggcctcac
     8641 gcctgggcac cctgacagcg gccgtgcggc gcccacggag gttagctgca aggcccgcgg
     8701 attccttacg tctctttgcg gacgatgggg atggctggca tccgggccgt cttgcggatg
     8761 gcctgtggga ggtcactcac ggggccgtgg atgacgttcg tcaggggagc aacttctcca
     8821 caccggcagc gccctcttcc tcgagaaggc ggcgtgcctg ttcaagcctc tccggcgtga
     8881 tgtcgtggcc agatgccagt actacgtctt ccgggtcggg caactcttcc gttcccgtgt
     8941 ggagaaccgc atccggcgtg tgcggacccc actcatcgtc gccagtgcct tcagacatca
     9001 tttctcccgc gtttgaatat gtgagacggg cgtcgctccg caccatgttc ggcgtgagag
     9061 atcaaccctt gaaatgggcg gcaatcttca ctgatcgccg gtgctctcgt gctggttcgc
     9121 agcccaggca tacgcctctg ccaggtcctt caaggccgct gcgaccgctc catgatcctt
     9181 gtgctccgcc tgctgtcggg cttgagacgc gatggcctcg aaaagcgctt ctcgagcatc
     9241 ctgttgcgcc gtggagtcag cactgctcac gatgtcctcc tgatttccgt ccgggcaaga
     9301 aaccagaccc agccgactca cgccggttct cgttgcaacc aatgcatctg gcaaaggggt
     9361 tggcgcgccg ggccggtcag taagttgttc ctacgcgaat gctattgcgg cgttgatcta
     9421 cagctcatct atgcaggatc gatgcccccg tggagctgtc ggtcctgcta gtgcagcggc
     9481 ttgcccgcga gggccaggtg ggcttcgccg agcgcctcgt tctgcgtcgg gtgggcgtgg
     9541 atgagctggg cgacctcggt gggcagcgcc tcccagttgt agatcagctg ggcctcgccg
     9601 acctgctcgc ccatgcggtc gccgaccatg tggacgccga ccacggcacc gtccttgacc
     9661 tggacgagct tgatctcgcc cgcggtgttg aggatcttgc tcttgccgtt gcccgcgagg
     9721 ttgtacttca gagcgacgac cttgtccgca ccgtagatct ccttggcctt ggcctcggtg
     9781 atgcccacgg aggcgacctc ggggtggcag taggtcaccc gcgggacgcc gtcgtagtcg
     9841 atcgggacgg tcttcaggcc ggccagacgc tccgccacca ggatgccctc ggcgaagccg
     9901 acgtgcgcga gctggagcgt ggggacgagg tcaccgacgg cggagatggt gggcacgttg
     9961 gtgcgcatgt actcgtcgac caggacgtag ccgcggtcca tcgcgacgcc ctgctcctcg
    10021 tagccgaggc cctgcgagac gggcccgcgg ccgacggcga ccagcaggac ctcggcctcg
    10081 aactccttgc cgtcggcgag ggtgaccttg accccgtcct gggtgtactc ggccttcgag
    10141 aagaaggtgc ccaggttgaa cttgatgccg cgcttgcgga acgcgcgctc gagaagcttg
    10201 gaggagttct cgtcctcgac cgggacgagg tgcttgaggc cctcgatgac cgtgacgtcc
    10261 gcaccgaagg acttccaggc ggaggcgaac tcgacgccga tgacaccgcc gcccaggatg
    10321 atcgcggact tcggcacgcg gtccaggacg agggcgtggt cggaggagat gatccggttg
    10381 ccgtcgatct ccaggcccgg cagcgacttc ggcaccgaac cggtcgccag gagcacgtgg
    10441 cggccctgca cccgctggcc gttcacgtcg atcgaggtgg gcgacgacag ccggccctca
    10501 ccctcgatat aggtgatgtc acgtgccttc acctggccgg tcagacctcg gcagagtccg
    10561 gcgaccgtat cgtctttgta gcgctgcacg gacgccatgt cgatgctttc gaaggaggcc
    10621 ctcaccccga acaactccgc gtcgcgggtc cggtcggcga tctcgcccgc gtgcagcagg
    10681 gccttggtgg ggatgcaccc gttgtgcagg caggtacccc ccagcttgta cttctccacc
    10741 atggccgtgc gcagaccgag ttgggcaccg cgcaatgcgg ctgcgtaccc tgcgctgcca
    10801 ccaccgagca cta
//
