LOCUS       NZ_CP034669            10873 bp    DNA     circular CON 19-MAY-2024
DEFINITION  Corallococcus coralloides strain B035 chromosome, complete genome.
ACCESSION   NZ_CP034669
VERSION     NZ_CP034669.1
KEYWORDS    RefSeq.
SOURCE      Corallococcus coralloides
  ORGANISM  Corallococcus coralloides
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Corallococcus.
REFERENCE   1  (bases 1 to 9587888)
  AUTHORS   Bouhired,S.M., Rupp,O., Blom,J., Schaeberle,T.F., Kehraus,S.,
            Schiefer,A., Pfarr,K., Goesmann,A., Hoerauf,A. and Koenig,G.M.
  TITLE     Complete Genome Sequence of the Corallopyronin A producing
            Myxobacterium Corallococcus coralloides B035
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9587888)
  AUTHORS   Bouhired,S.M., Rupp,O., Blom,J., Schaeberle,T.F., Kehraus,S.,
            Schiefer,A., Pfarr,K., Goesmann,A., Hoerauf,A. and Koenig,G.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2018) Institute for Pharmaceutical Biology,
            University of Bonn, Nussallee 6, Bonn, NRW 53123, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-SEP-2017
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 350.5x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004104415.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:23:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,767
            CDSs (total)                      :: 7,695
            Genes (coding)                    :: 7,616
            CDSs (with protein)               :: 7,616
            Genes (RNA)                       :: 72
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 55 of 79
            Pseudo Genes (internal stop)      :: 4 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:35:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5414951
            Orig. end                         :: 5425824
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034669.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Gabriele M. Koenig,
            Institute for Pharmaceutical Biology, University of Bonn.
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10873
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="25"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10873
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10873
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5873](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5873
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     tRNA            complement(46..119)
                     /anticodon=(pos:complement(5415034..5415036),aa:Met,
                     seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="EJ065_RS21480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="EJ065_4258"
                     /product="tRNA-Met"
     gene            complement(46..119)
                     /locus_tag="EJ065_RS21480"
                     /old_locus_tag="EJ065_4258"
     misc_feature    142..156
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.98"
                     /tool="antismash"
     tRNA            complement(198..271)
                     /anticodon=(pos:complement(5415186..5415188),aa:Met,
                     seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="EJ065_RS21485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="EJ065_4259"
                     /product="tRNA-Met"
     gene            complement(198..271)
                     /locus_tag="EJ065_RS21485"
                     /old_locus_tag="EJ065_4259"
     gene            complement(375..2381)
                     /locus_tag="EJ065_RS21490"
                     /old_locus_tag="EJ065_4260"
     CDS             complement(375..2381)
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397285.1"
                     /locus_tag="EJ065_RS21490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EJ065_4260"
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="WP_128797524.1"
                     /transl_table=11
                     /translation="MSDEKSGGNSGGPGGFGPKKPKATFGDVMLGIPSGGQGNERGGGR
                     GGRDEKSGPGRGDRGPRDAQSQPRPQGDAPRGGGERGGGRGGERRGGGGGGERRPSGPM
                     VVVKRASGSIETRALEGEKPAEATATAEQTGADAQASAASTPAPAPRPVTPAPVPSSPL
                     YEEVPETESFADMFEAQVKDGGAPGRRGVRIGEKVAGTIFQLGADTAFVSLDGSAKSEA
                     MIELRELKDDEGILRFGVGDRLEAHVVEMGARGIQLSRALAKGNASFAMLAEARASGMP
                     VEGLVLSVNKGGVEVAIGETRAFCPISQLDIRFVEKPDQFIGEKLQFRVTEVRDRNVVL
                     SRRSLLEDEQRRLATETRKTLAVGKTVKGKVSGVRDFGVFVDLGGVEGMVPVSELSYTR
                     VAHPSDVVKQGDEVEVEILRMEEGQPNSPDKSKQKERITLSLRSRQEDPFKKALEEIKE
                     GDRMQGKVVRLQPFGAFVELRPGVDGLVHISALSDRRIAHPRDAVKEGEVIWVSVEKID
                     PNDKRIGLRRISEEEAQRPPEERPAKEAQASAPKEPSAPRPKVGAVVVGKVDRLEPYGV
                     FLAFPGGKGLIPASETGTDRGTDMRKHFSIGQELKVAIIDIDASGKIRLSIPGAIRAEE
                     RAEVEAWQKTQQPQGAGKKGFGTFADLLSKLGK"
     gene            complement(2610..4343)
                     /gene="murJ"
                     /locus_tag="EJ065_RS21495"
                     /old_locus_tag="EJ065_4261"
     CDS             complement(2610..4343)
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="murJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940155.1"
                     /locus_tag="EJ065_RS21495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EJ065_4261"
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="WP_128797525.1"
                     /transl_table=11
                     /translation="MTAPAPASPPSAGSPAAPLPSSPERSAGGRGAMLVGIGILASRLM
                     GLVRERVFAHYLGNAEAAAVFKAALRIPNFLQNLFGEGVLSGSFIPVYAQLLGKKDADE
                     ADRVAGAVFGLLAMATAVMVALGMLATPLFVDLIAPGFEGGERDLAVRLVRILFPGTGF
                     LVLSAWCLGILNSHRRFLLSYLAPVVWNVVIIATLLVVGSLHGTSGGRAAEEAVTEWLA
                     YGVVLGSFLQFAVQVPTVMRLLGHFRPVVSLASASVRQVLKNFGPVVLGRGVVQFSAWV
                     DTAFASLISNRALSSLLYAQTLYLIPVSLFGMAVSAAELPEMARATAEGEAQAHSKLRS
                     RIDAGSRRIAFWVVPSAVALFFLGDLVSGALLQTGRFGASDSRYLWYLLMGAAVGLVAS
                     TVGRLYASAFYALKDPKTPLRYAIVRVSLGTVKAWFLALWLPERLGLPRELGAAFLTLS
                     SGIVAWVESTLLRRKLRSIVGPVGLPSGLLPRLYGAAVVGGLVALAVKQGLTGLLGPMP
                     GVGAEWGGTLLVPPRLHPVLGLLATAVPFGVVYFAISAALGVPEAGAVFRKVGRKLGLV
                     R"
     gene            4449..4937
                     /locus_tag="EJ065_RS21500"
                     /old_locus_tag="EJ065_4262"
     CDS             4449..4937
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397287.1"
                     /locus_tag="EJ065_RS21500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EJ065_4262"
                     /product="ferritin family protein"
                     /protein_id="WP_128797526.1"
                     /transl_table=11
                     /translation="MAGKSDTERSDVARIRAVLARELETINEYEAFAEDSSLPEVKAFF
                     LHLAAEEKEHVSEATHMLRMLDQGQDAHFARPFVPGHFQAAVGAVPSEPAVPAAEPAPL
                     VTRAPPALGRLANEPLTSLPPQRLIYGVPAPPPSANGHPLTIGSLRRGGGSGGSGGGR"
     gene            5001..5873
                     /gene="encA"
                     /locus_tag="EJ065_RS21505"
                     /old_locus_tag="EJ065_4263"
     CDS             5001..5873
                     /codon_start=1
                     /gene="encA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397288.1"
                     /locus_tag="EJ065_RS21505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EJ065_4263"
                     /product="encapsulin nanocompartment shell protein EncA"
                     /protein_id="WP_120566121.1"
                     /sec_met_domain="Linocin_M18 (E-value: 5.4e-83, bitscore:
                     276.8, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDFLGHAENPLREEEWARLNETVIQVARRSLVGRRILDIYGPLG
                     AGVQTVAYDEFQGVSPGAVDIVGEQETAMVFTDVRKFKTIPIIYKDFLLHWRDIEAART
                     HNMPLDVSAAAGAAALCAQQEDELIFYGDQRLGYEGLMTANGRLTATLGDWTAPGGGFQ
                     TIVEATRKLNEAGHFGPYAVVLSPRLYSQLHRIYEKTGVLEIQTISQLAADGVYQSNRL
                     RGESGVVVSTGRENMDLAVAMDMVAAYLGASKMNHPFRVLESLLLRIKHPDAICTLEGA
                     TPAAPPARR"
     gene            5898..7322
                     /locus_tag="EJ065_RS21510"
                     /old_locus_tag="EJ065_4264"
     CDS             5898..7322
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940152.1"
                     /locus_tag="EJ065_RS21510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EJ065_4264"
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="WP_014397289.1"
                     /transl_table=11
                     /translation="MAKVLVIDDETNLRKVLAALLRRDGFDVTVAENGEQGLAEFHKNG
                     ADIVVTDLVMPKLGGMEVLAAVRAANPDVPVIIITAHGTVDSAVEAIKAGAFDYITKPF
                     DQAELSSVVAKAAKTNESAKRSVRADHKARSAIIGESPQIQDVYKIIDKVADTPSTVLI
                     TGESGTGKELIATALHGASSRRDKPFIKINCAAIPATLLESELFGYEKGAFTGAVTSKP
                     GRFELADEGTLFLDEIGEIPVEMQVKLLRALQEGEFERVGGIKTTRVNVRLVAATNRDL
                     QAEIEAGRFRKDLYYRLAVVPIVLPALRERRGDIPMLAAHFVDKYNRRLHKKIEGIADD
                     ALALLQAYAWPGNIRELENLIERVLLFADGPLITAKDLPEPVRGGAGVQAGAQVAASLG
                     TLDVPVGEVGLKDIVRMKAAELERDLIVKKLEETGGNVTRAARLLQISRKSLQTKMKEF
                     GLRDTTPDGQEDGPDE"
     gene            7397..8428
                     /locus_tag="EJ065_RS21515"
                     /old_locus_tag="EJ065_4265"
     CDS             7397..8428
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397290.1"
                     /locus_tag="EJ065_RS21515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EJ065_4265"
                     /product="M23 family metallopeptidase"
                     /protein_id="WP_128797527.1"
                     /transl_table=11
                     /translation="MRPNLPTLGGPPKRNPFGPVVAVSVILGAAAGGVWWWKQRMAEVP
                     LDVAAQTASALDAGTVAQAPVAPPAPTDPVKAAGLERVSIRIEGPLETALVQASDATVG
                     PALAQVVTRTLVWWVRVPGEILRGDTLDVLFQRRPNEEPLVYAVRFVSGKTGQTHRAYR
                     FTPAGEANARYYLPGGDELELRMEKSPIDSYEQITSLLRDGRGHKGVDFRAPVGTTVRA
                     PFAGVIKRKNWNFGSNGNCIELVETGGKGRRALFLHLAELPKSIQAGMRVNAGQVLAQS
                     GNTGHSFAPHLHYQLMLGENRVLDPFDQHKTFRASLTAAHKGAFDTEVQRLDGLLGTSV
                     AGK"
     gene            8507..9805
                     /locus_tag="EJ065_RS21520"
                     /old_locus_tag="EJ065_4266"
     CDS             8507..9805
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553582.1"
                     /locus_tag="EJ065_RS21520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EJ065_4266"
                     /product="hypothetical protein"
                     /protein_id="WP_128797528.1"
                     /transl_table=11
                     /translation="MHRFRAVIAALTLGVPFAASAAEITRIASSFEDDDPFDLFLDVGF
                     ERTQTRAKIVREQLRGGDATDVSEVNELWYKGTDARLNLAVAFGLWKDLEFSFKLPIVF
                     QQNETWNFVSGTNQDNSTITNSCYNADGSPYSAEGCVGRLFQVPQESYRGGLGNVHFGL
                     AYAFFNQEKDDTKPTWIIGIDYEAPTAKQRDPSLDNTDPENPATDGRGNVGDRVHKYQL
                     YTTFSRKMGVAEPYFKASHTIPVRGPGAYSNCDQSGLPSPGNLGAPYNCYKEPWTRQET
                     GIKAPSQTALTFGMELVPFENAARSQKFTLDLRLLGNYVGRGRYYNELSSALRKLLTSE
                     DYLQVGGQVGVTARAAQAFTIRASGNFLYNTDHLLTSEAIGQDLNGDGQVDTAPGSDEL
                     NPTFDWRYDLVSRRFRAIQSTTFRFDLGATFSF"
     gene            complement(9849..10682)
                     /gene="rsmI"
                     /locus_tag="EJ065_RS21525"
                     /old_locus_tag="EJ065_4267"
     CDS             complement(9849..10682)
                     /EC_number="2.1.1.198"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rsmI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397292.1"
                     /locus_tag="EJ065_RS21525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EJ065_4267"
                     /product="16S rRNA (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="WP_120576719.1"
                     /sec_met_domain="TP_methylase (E-value: 3.3e-30, bitscore:
                     104.4, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGTLYLVATPIGNLGDVTARALETLRTVGLLACEDTRHSRILLD
                     HFGITGKDLVSLPAFAEGQRAGRILDRIEAGEDCALVTDAGSPGISDPGEKLVAEALER
                     GLKVEPVPGPTALVAALSASGLPTGRFHFLGFLPRKGPERRAMLDEVAPLSATCVLYES
                     PRRLAETLVDLQEAWGDRRACVARELTKLHEEFVRGPLSALVARYAGEETRGEVVVLVE
                     GRTGEQRWSEDELRRALEAGLARGEKLKPLSTELARRAGWPGQEVYRLGLGLKQR"
ORIGIN
        1 aaaaagcgaa agggccccgg tgtttccacc ggggccctct cagagttgcg ggggcaggat
       61 ttgaacctgc gaccttcggg ttatgagccc gacgagctac caggctgctc caccccgcgt
      121 caccgtgacg cggcactgct gttatcacct tgaacacccg gggtcgaaac tttcatttcg
      181 accccgggct tttcaagttg cgggggcagg atttgaacct gcgaccttcg ggttatgagc
      241 ccgacgagct accaggctgc tccaccccgc ggcgagaacg agagggtaag taccgaagcc
      301 tctcgctacc gtcaacacct atctcggcgc cccatcgcat tgtctcgcgc gagcgccgag
      361 atggcgtgac gtcactactt gcccagcttg ctgagcagat ccgcgaaggt gccgaagccc
      421 ttcttgccag cgccctgcgg ctgctgcgtc ttctgccacg cctccacctc ggcgcgctcc
      481 tccgcgcgga tggcgccggg gatggacagg cggatcttcc cggaggcgtc gatgtcgatg
      541 atcgccacct tcagttcctg gccgatggag aagtgcttgc gcatgtccgt gccgcggtcc
      601 gtgcccgtct cgctggccgg gatgaggccc ttgccgcccg ggaacgcgag gaacacgccg
      661 tagggctcca gccgatccac cttgccgacg acgaccgcgc ccaccttggg acggggcgcc
      721 gagggctcct tgggagccga cgcctgggcc tccttcgcgg gacgctcctc gggaggacgc
      781 tgggcctcct cctcggagat gcggcgcagg ccgatgcgct tgtcattggg gtcgatcttc
      841 tcgacggaga cccagatgac ctcgccttcc ttcaccgcgt cgcgcgggtg cgcgatgcgc
      901 cggtcgctca gcgcggagat gtgcaccagg ccgtccacgc ccgggcgcag ttccacgaac
      961 gcgccgaagg gctggagccg gacgaccttg ccctgcatgc ggtcgccctc cttgatctcc
     1021 tccagcgcct tcttgaacgg atcctcctga cgcgagcgca gcgacagggt gatgcgctcc
     1081 ttctgcttgg acttgtccgg ggagttgggc tggccctcct ccatgcggag gatctccacc
     1141 tccacctcgt cgccctgctt caccacgtcg ctcgggtgag cgacgcgcgt gtaggagagc
     1201 tcggagacgg ggaccatgcc ctccacgccg cccaggtcca cgaacacgcc gaagtcgcgc
     1261 acgccggaga ccttgccctt gaccgtcttg cccacggcca gggtcttgcg cgtctccgtg
     1321 gccagccggc gctgctcgtc ctccagcagc gagcggcggg acagcaccac gttgcggtcg
     1381 cgcacttccg tcacgcggaa ctggagcttc tcgccgatga actgatccgg cttctccacg
     1441 aagcggatgt ccagctggct gatggggcag aaggcgcgcg tctcaccgat ggcgacttcc
     1501 acgccaccct tgttcacgct gaggaccagg ccctccaccg gcatgccgga ggcgcgggcc
     1561 tccgcgagca tcgcgaagga cgcgttgccc ttggccagcg cgcggctgag ctggatgccc
     1621 cgggcaccca tctccacgac gtgcgcctcc aggcggtcac ccacgccgaa gcgcaggatg
     1681 ccctcgtcgt ccttgagctc gcgcagctcg atcatcgcct ccgacttcgc ggagccgtcc
     1741 agcgacacga acgcggtgtc cgcgcccagc tggaagatgg tgccggcgac cttctcgcca
     1801 atgcgcacgc cgcggcggcc gggagcgcca ccgtccttga cctgcgcctc gaacatgtcc
     1861 gcgaaggact cggtctccgg gacctcttcg tacagcgggc tggacggcac gggggccggc
     1921 gtcaccggac gcggggccgg agccggcgtc gaggccgcgg aggcctgcgc gtcggcgccc
     1981 gtctgctccg cggtcgcggt cgcctcggcg ggcttctcac cctccagggc gcgcgtctcg
     2041 atggagcccg aggcccgctt cacgaccacc atggggccgg acggacggcg ctcgccacct
     2101 cctccgccac cccggcgctc gccgccacgg ccaccgccgc gctcgccgcc accgcggggc
     2161 gcgtcgccct ggggacgggg ctgcgactgg gcgtcgcgag gaccgcgatc accgcggccc
     2221 ggcccactct tctcatcgcg gccgccacgg ccaccgccgc gctcgttgcc ctggcccccc
     2281 gaagggatgc ccagcatcac gtcgccaaac gtggccttcg gcttcttggg accgaaaccg
     2341 ccaggaccgc ccgaatttcc gccgctcttc tcgtcgctca ctgccgagac cttccgttga
     2401 cgatttccga cccgggactt ccttgaagtc agggcctctt gaaaaaggcg gcggacatta
     2461 cacgcgcgcc atgcccggag gaagccgcat gcggccatcc ccttcacaat cggcctccta
     2521 tcccaacgaa aaggggcctg tcctgcattc cgtttgagcc cccaggcacc gcctggaggg
     2581 cagccgtccc accgggcgcc gcgccgcgtc taccggacga gccccagctt ccggcccacc
     2641 ttgcggaaga cggcgcccgc ctccgggacg cccagcgcgg cggagatggc gaagtagacg
     2701 acgccgaagg gcaccgccgt cgccagcagg cccagcaccg ggtgcagccg gggcggcacc
     2761 aggagggtcc cgccccactc cgcccccacc ccgggcatgg ggcccaggag gccggtgagc
     2821 ccctgtttga cggccagggc cacgagcccg cccaccaccg ccgcgccgta gagccggggc
     2881 agcaggcccg aaggcaggcc cacgggcccg acgatggagc gcagcttgcg gcgcaggagc
     2941 gtgctctcca cccaggcgac gatgccgctg gacagggtga ggaacgccgc gcccagctcc
     3001 cggggcaggc ccagccgctc cggcagccac agcgccagga accaggcctt caccgtcccc
     3061 agggacacgc gcacgatggc gtagcgcagg ggcgtcttcg gatccttcag cgcgtagaag
     3121 gcggaggcgt agagccgtcc cacggtggac gccaccaggc ccaccgccgc gcccatcagc
     3181 aggtaccaga ggtagcgcga gtccgaggcg ccgaagcggc ccgtctgcag cagcgcgccg
     3241 ctcaccaggt cccccaggaa gaagagcgcg acggcggagg gcaccaccca gaaggcgatg
     3301 cggcgcgagc ccgcgtcgat gcggcttcgc agcttcgagt gcgcctgggc ctccccttcc
     3361 gccgtggcgc gcgccatctc cggcagctcc gcggcggaca ccgccatgcc gaagaggctc
     3421 accgggatga ggtagagggt ctgcgcgtag aggagcgacg acagcgcgcg gttggagatg
     3481 agcgacgcaa aggcggtgtc cacccacgcg ctgaactgca ccacgccgcg ccccagcacc
     3541 accgggccga agttcttcag cacctggcgc acggaggcgc tggccagcga caccaccggg
     3601 cggaagtggc ccaacaggcg catcaccgtg ggcacctgca ccgcgaactg gaggaagctg
     3661 cccagcacca cgccgtaggc cagccactcc gtcacggcct cttccgcggc gcgtcccccg
     3721 gacgtgccat gcaggctgcc cacgaccagc agcgtggcga tgatgacgac gttccacacc
     3781 acgggcgcca ggtaggacag caggaagcgc cggtggctgt tgaggatgcc gaggcaccac
     3841 gcgctcagca ccaggaagcc cgtgcccggg aagaggatgc gcaccagccg cacggccagg
     3901 tcgcgctcgc cgccctcgaa gcccggcgcg atgaggtcca cgaacagcgg cgtggccagc
     3961 atgcccagcg ccaccatcac ggcggtggcc atggccagca ggccgaacac cgcgcccgcg
     4021 acccggtccg cctcgtccgc gtccttcttg cccagcaact gggcatagac ggggatgaag
     4081 gagcccgaca gcacgccttc gccgaagagg ttctggagga agttggggat gcgcagcgcg
     4141 gccttgaaga cggcggcggc ctccgcgttg cccaggtagt gcgcgaagac ccgctcgcgc
     4201 accagcccca tcagccgcga cgccaggatg ccaatgccca ccagcatcgc gccccggcct
     4261 cccgcgctgc gttcagggct ggacggaaga ggggcggccg gagagccggc ggacgggggc
     4321 gaggcgggtg cgggggcggt cacgaagggg cggactctac gtccggcgtt cttcggcatg
     4381 cagcaggaaa cccttcgacc gctgtttcac ttgacggtca ccccacgggt tgcgagggtc
     4441 cgcccccaat ggccggaaag tccgacaccg agcggtccga cgtcgcgcgc atccgcgccg
     4501 tcctggcgcg cgaactcgag accatcaacg agtacgaggc gttcgccgag gactcctccc
     4561 tcccggaagt gaaggccttc ttcctccacc tggcggccga ggagaaggag cacgtgtccg
     4621 aggcgaccca catgctccgc atgctcgacc agggtcagga cgcgcacttc gccaggccct
     4681 tcgtccccgg ccacttccag gccgctgtcg gggctgtccc ttcggagccc gccgtgcccg
     4741 ccgccgaacc cgcgcccctg gtgacgcgcg cgccgcccgc attgggccgc ctggcgaacg
     4801 agcccctgac gtccctgccg ccccagcgcc tcatctatgg cgtgcccgcg ccgccgcctt
     4861 cggccaacgg ccatccgctc accatcggca gcctgcgccg aggcggtgga agcggcggct
     4921 ccggtggcgg tcgctagaac cctttcgttc cctgccttct cctctcccct cctctgccgc
     4981 cgctcgtgac ggagacaccg atgcctgact tccttggaca tgccgagaac ccgctgcgcg
     5041 aagaggagtg ggcgcgcctc aacgagacgg tgatccaggt ggctcgccgt tcgctggtgg
     5101 gccgccgcat cctggacatc tacgggccgc tgggcgcggg cgtgcagacg gtggcgtacg
     5161 acgagttcca gggcgtgtcc ccgggcgcgg tggacatcgt cggcgagcag gagaccgcga
     5221 tggtcttcac cgacgtccgc aagttcaaga ccatccccat catctacaag gacttcctgc
     5281 tgcactggcg ggacatcgag gcggcgcgca cgcacaacat gccgctggac gtgtccgccg
     5341 ccgccggcgc cgccgcgctg tgcgcgcagc aggaggacga gctcatcttc tacggcgacc
     5401 agcgcctggg ctacgagggc ctgatgacgg ccaacgggcg cctcaccgcc acgctggggg
     5461 actggacggc gccgggcggc ggcttccaga ccatcgtgga ggccacgcgc aagctcaacg
     5521 aggccggcca cttcgggccc tacgccgtgg tgctgtcgcc gcgcctgtac tcgcagctgc
     5581 accgcatcta cgagaagacg ggcgtgctgg agatccagac catcagccag ctggcggcgg
     5641 atggcgtcta ccagtccaac cgcctgcgcg gtgagtccgg cgtggtggtg tccacgggcc
     5701 gggagaacat ggacctggcg gtggccatgg acatggtcgc ggcctacctg ggcgccagca
     5761 agatgaacca cccgttccgc gtgctggagt cgctgctcct gcgcatcaag cacccggacg
     5821 ccatctgcac gctggaaggc gccaccccgg ccgctccccc ggcgcgccgt taaatcgcgg
     5881 tccaagcgcg gagattcatg gccaaggtcc tggtcatcga cgacgagacg aacctgcgca
     5941 aggtgctggc cgccctgctg cgccgcgacg ggttcgacgt caccgtggcg gagaacggcg
     6001 agcagggcct ggcggagttc cacaagaacg gcgcggacat cgtcgtcacc gacctggtga
     6061 tgcccaagct gggcggcatg gaggtgctcg ccgccgtgcg cgccgccaac ccggacgtgc
     6121 cggtgatcat catcaccgcg cacggcaccg tggactccgc ggtggaggcc atcaaggcgg
     6181 gcgcgttcga ctacatcacc aagccgtttg atcaggcgga gctgtcctcc gtcgtggcca
     6241 aggccgccaa gacgaacgag agcgccaagc gctccgtgcg cgcggaccac aaggcccggt
     6301 ccgccatcat cggcgagtcg ccgcagatcc aggacgtcta caagatcatc gacaaggtcg
     6361 cggacacgcc ctccacggtg ttgatcaccg gcgagagcgg cacgggcaag gagctcatcg
     6421 ccaccgcgct gcacggcgcg tccagccgcc gcgacaagcc gttcatcaag atcaactgcg
     6481 ccgccatccc cgccacgctg ctggagagcg agctgttcgg ctacgagaag ggcgccttca
     6541 ccggcgccgt cacgtccaag cccggccgct tcgagctggc cgacgagggc accctcttcc
     6601 tggacgagat tggcgagatc cccgtcgaga tgcaggtgaa gctcttgcgc gcgctccagg
     6661 agggcgagtt cgagcgcgtg ggcggcatca agacgacgcg cgtgaacgtg cgcctggtgg
     6721 ccgccaccaa ccgcgacctc caggcggaga tcgaagcggg ccgcttccgc aaggacctgt
     6781 actaccggct ggccgtggtg cccatcgtgc tgcccgcgct gcgcgagcgc cgcggcgaca
     6841 tcccgatgct cgccgcgcac ttcgtggaca agtacaaccg gcggctccac aagaagattg
     6901 agggcatcgc cgacgacgcg ctcgcgctgc tccaggcgta cgcgtggccg ggcaacatcc
     6961 gcgagctgga gaacctcatc gagcgcgtgc tgctcttcgc cgatggcccc ctcatcaccg
     7021 cgaaggacct gccggagccg gtgcgcgggg gagcgggcgt gcaggcgggg gcacaggtcg
     7081 ccgcctcgct gggcacgctg gacgtccccg tgggcgaggt gggcctcaag gacatcgtgc
     7141 gcatgaaggc cgcggagctg gagcgggacc tcatcgtcaa gaagctggag gagacgggcg
     7201 gcaacgtcac gcgcgccgcg cgcctcctgc agatcagccg caagtcgctc cagacgaaga
     7261 tgaaggagtt cggcctgcgc gacaccaccc cggacggtca ggaagacggc ccggacgagt
     7321 agcctggggg ccgaaatatc gccttttcgc cggcggttcc ggcccccgcg taccttctta
     7381 ccctcaggga gcctgaatgc ggccgaatct tcccaccctc ggtggaccac cgaagcgcaa
     7441 tcccttcgga ccggtggttg ccgtctccgt catcctcggc gcggccgcgg gtggcgtgtg
     7501 gtggtggaaa cagcgcatgg cggaggtccc cctggacgtc gccgcgcaga ccgcgtccgc
     7561 gctggacgcc ggcaccgtgg cccaggcccc cgtggccccg cccgcgccca ccgaccccgt
     7621 gaaggccgcg ggcctggagc gcgtgtccat ccgcatcgag ggcccgctgg agacggcgct
     7681 cgtccaggcc tccgacgcca ccgtgggccc cgccctggcg caggtggtga cgcgcacgct
     7741 ggtgtggtgg gtgcgcgtac ccggcgaaat cctccggggg gacaccctgg acgtgctctt
     7801 ccagcgccgc cccaacgagg agccgctggt gtacgcggtg cgcttcgtga gcggcaagac
     7861 gggccagacg caccgcgcct accgcttcac gcccgcgggt gaggcgaacg cccgctacta
     7921 cctgcccggc ggcgacgagc tggagctgcg gatggagaag tcccccatcg acagctacga
     7981 gcagatcacc tcgctcttgc gcgacggccg cggccacaag ggcgtggact tccgcgcgcc
     8041 ggtgggcacg acggtccggg cgcccttcgc gggcgtcatc aagcgcaaga actggaattt
     8101 tggcagcaac ggcaactgca tcgagctggt ggaaaccggg ggcaagggtc gccgcgccct
     8161 cttcctgcac ctggcggagc tgcccaagag catccaggcc gggatgcgcg tgaacgcggg
     8221 ccaggtgctg gcccagagcg gcaacacggg ccactccttc gccccccacc tgcactacca
     8281 gctcatgctg ggcgaaaacc gggtcctgga ccccttcgac cagcacaaga ccttccgcgc
     8341 gtccctgacg gccgcccata agggcgcctt cgacaccgag gtccagcggc tggacggatt
     8401 gctgggaacc tccgtcgcgg ggaagtaaag ccccacccat ttcttgacac tcctccgacg
     8461 gcacggctag ctggcagccc tgggccgtgg tcgtcggagg tagtggatgc acaggtttcg
     8521 cgccgtcatc gccgcgctga cgctgggcgt gcctttcgcc gccagcgcgg cggagatcac
     8581 ccggattgct tcctcgttcg aggatgatga tccgttcgac ctcttcctcg atgtgggctt
     8641 cgagcgcacg cagacgcgcg cgaagatcgt ccgcgagcag cttcgcggcg gtgacgccac
     8701 cgacgtctcg gaggtcaacg agctctggta caagggcacg gacgcgcggc tgaatctggc
     8761 cgtggccttc ggcctgtgga aggacctgga gttcagcttc aagctgccca tcgtcttcca
     8821 gcagaacgag acctggaact tcgtgtccgg cacgaaccag gacaactcca ccatcaccaa
     8881 cagctgctac aacgcggacg gctcgcccta ctccgccgag ggctgcgtgg gccggctgtt
     8941 ccaggtcccc caggagagct accgcggcgg cctgggcaac gtgcacttcg gcctggcgta
     9001 cgccttcttc aaccaggaga aggacgacac caagcccacg tggatcatcg gcatcgacta
     9061 cgaggcgccc accgccaagc agcgcgaccc gagcctggac aacactgatc cggaaaaccc
     9121 ggcaactgat gggcgaggca acgtgggcga ccgcgtccac aagtaccagc tctacacgac
     9181 cttctcgcgg aagatgggcg tggcggagcc gtacttcaag gcgtcccaca ccatcccggt
     9241 gcgcggcccc ggcgcctact ccaactgcga tcagtccggc ctcccctccc cggggaacct
     9301 gggcgcgccg tacaactgct acaaagagcc gtggacccgc caggagacgg gcatcaaggc
     9361 cccctcacag acggcgctca ccttcggcat ggagctggtg cccttcgaga acgcggccag
     9421 gtcccagaag ttcaccctgg acctgcggct cctgggcaac tacgtgggcc gcggccggta
     9481 ctacaacgag ctgagctccg cgctgcgcaa gctgctcacc tccgaggact acctccaggt
     9541 gggtggccag gtgggcgtca ccgcccgcgc cgcccaggcc ttcaccatcc gcgcctccgg
     9601 caacttcctc tacaacacgg accacctgct cacgtccgag gccattggcc aggacctgaa
     9661 cggcgacggc caggtggaca cggccccggg ctcggatgag ctgaacccca cgttcgactg
     9721 gcgctacgac ctggtgtccc gccgcttccg ggccatccag agcaccacgt tccgcttcga
     9781 cctgggcgcg accttcagct tctgaagtcc cgcccccgag gcagcggtgg gcctcgccgg
     9841 ccgctgcttc agcgctgctt cagccccagg cccagccggt acacctcctg gccgggccac
     9901 cccgcgcggc gggccagctc cgtgctcagc ggcttgagct tctctccgcg agccagcccc
     9961 gcctccagcg cccggcgcag ctcgtcctcg ctccagcgct gctcgccggt gcggccctcc
    10021 accagcacca ccacctcgcc ccgggtctcc tcgcccgcgt agcgcgccac gagcgcggac
    10081 agcgggcccc ggacgaactc ctcgtgcagc ttggtcagct cgcgcgccac gcacgcgcgg
    10141 cggtcgcccc aggcctcctg gaggtccacc agcgtctccg ccaggcggcg cggggactcg
    10201 tagaggacgc aggtggcgga caggggggcc acctcgtcca gcatggcccg gcgctccggc
    10261 ccctttcgcg gcaggaagcc caggaagtgg aagcgcccgg tgggcaggcc ggacgcgctc
    10321 agggcggcca ccagcgccgt gggcccgggc accggctcca ccttcaggcc gcgctccagc
    10381 gcctccgcca ccagcttctc tcccgggtcg ctgatgccgg ggctgcccgc gtcggtgacg
    10441 agcgcgcagt cctcccctgc ctcgatgcgg tccaggatgc gcccggcgcg ctgcccctcc
    10501 gcgaaggcgg gcaggctcac caggtccttc ccggtgatgc cgaagtggtc caacaggatg
    10561 cgcgagtgcc gggtgtcctc gcacgcgagg aggcccacgg tgcgcagcgt ctccagggcc
    10621 cgggcggtga cgtcccccag gttcccgatg ggcgtggcca cgaggtagag cgttccagcc
    10681 aagggacgga ttcctttaca tgccagcgtc gaaggcggcg ggcaggtgct ccacccgggc
    10741 gtgctcgccc tggcccacga cggagatgac gtcgaagcgc accatgcgcc cgtcgatgcc
    10801 gttctggaag aggtagtgca acgcggcctt caccacccgg cgctgcttgg cgaaggacac
    10861 ggtgtgcgac ggg
//
