LOCUS       NZ_RQJW01000019        10405 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces cyaneus strain CGMCC 4.1671 Scaffold16_1, whole genome
            shotgun sequence.
ACCESSION   NZ_RQJW01000019
VERSION     NZ_RQJW01000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces cyaneus
  ORGANISM  Streptomyces cyaneus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 161181)
  AUTHORS   Fang,B.-Z., Han,M.-X., Jiao,J.-Y., Xie,Y.-G., Zhang,X.-T., Liu,L.,
            Zhang,Z.-T., Xiao,M. and Li,W.-J.
  TITLE     Streptomyces sp. nov., a novel actinobacterium isolated from a karst
            cave sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 161181)
  AUTHORS   Fang,B.-Z., Han,M.-X., Jiao,J.-Y., Xie,Y.-G., Zhang,X.-T., Liu,L.,
            Zhang,Z.-T., Xiao,M. and Li,W.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2018) School of Life Sciences, Sun Yat-sen
            University, No. 135 Xingang West Road, Guangzhou, Guangdong 510275,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-OCT-2018
            Assembly Method                   :: SPAdes v. 3.6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004028635.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 03:28:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,717
            CDSs (total)                      :: 8,635
            Genes (coding)                    :: 8,413
            CDSs (with protein)               :: 8,413
            Genes (RNA)                       :: 82
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 77 of 222
            Pseudo Genes (incomplete)         :: 187 of 222
            Pseudo Genes (internal stop)      :: 12 of 222
            Pseudo Genes (multiple problems)  :: 50 of 222
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:58:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 91689
            Orig. end                         :: 102094
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RQJW01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    341..356
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.89"
                     /tool="antismash"
     misc_feature    817..832
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            891..1991
                     /locus_tag="EHO50_RS39325"
     CDS             891..1991
                     /NRPS_PKS="Domain: Aminotran_5 (73-259). E-value: 5.4e-26.
                     Score: 83.3. Matches aSDomain:
                     nrpspksdomains_EHO50_RS39325_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="EHO50_RS39325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_128436642.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.4e-21, bitscore:
                     70.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLTPLSAGRFAAIEDRVARLLGTEQDVVIMQGEALLPLE
                     GAIRGTAGPGTVALNVITGPYGQTFGDWLRDCGATVIDLAVPFHTAVTADQVREAFAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASIGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWVRMAANPRAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIEGDGLDAVMARHRAAALSTRAGAVALGGGLEPYVYDAGEAAPVA
                     TTLRAPAGVVASEVVARALAGDPALPLAAGGGALAKEMIRVNHYGVDAASGAVRGCVAA
                     LGAALAEKGLGVDVEGALRVVEEAWR"
     aSDomain        1110..1667
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EHO50_RS39325_Aminotran_5.1"
                     /evalue="5.40E-26"
                     /label="EHO50_RS39325_Aminotran_5.1"
                     /locus_tag="EHO50_RS39325"
                     /protein_end="259"
                     /protein_start="73"
                     /score="83.3"
                     /tool="antismash"
                     /translation="LRDCGATVIDLAVPFHTAVTADQVREAFAEHPEIDFVSLVHAEAA
                     TGNTNPVAEIGEVVRAHGALFYLDAVASIGAEPVLPDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSERAWVRMAANPRAPRRSYLSLLDWKERWIDGGRKALLHAPAQLEMLALEACVER
                     IEGDGLDAVMARHRAAALSTRAG"
     gene            2299..2832
                     /locus_tag="EHO50_RS39330"
     CDS             2299..2832
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002247.1"
                     /locus_tag="EHO50_RS39330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DinB family protein"
                     /protein_id="WP_128436643.1"
                     /transl_table=11
                     /translation="MTTNTPELLPDGRPLPLLTGDELPMLESWLAFHRATLELKCSGLD
                     DVQLRLASVESSELTLLGLVQHLAECERNWFQRVVGGLDVPPVYGEGNESGYALDAGRG
                     IGEALAVWRREIARGREVCAGRALESVGRIGDGPMAGVEVSLRWVLIHMIEEYARHNGH
                     ADILRERIDGVTGA"
     misc_feature    2847..2861
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.19"
                     /tool="antismash"
     misc_feature    complement(2958..2973)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2968..2983
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    3102..3114
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3139..3645
                     /gene="ectA"
                     /locus_tag="EHO50_RS39335"
     CDS             3139..3645
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140785.1"
                     /locus_tag="EHO50_RS39335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_128436644.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPAVADGAALWRIARDSKALDLNSSYSYLLWCRD
                     FADTSAVARDERGEPVGFVTGYVRPDRPQTLLVWQVAVDDTHRGRGIAAALLDGLVARA
                     GREYGVTSVETTITPGNTASERLFGAFAERHGAALEREVLFDAGLFPDGPHDPEVLYRI
                     GPLSP"
     gene            3701..4972
                     /gene="ectB"
                     /locus_tag="EHO50_RS39340"
     CDS             3701..4972
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.9e-74.
                     Score: 242.7. Matches aSDomain:
                     nrpspksdomains_EHO50_RS39340_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="EHO50_RS39340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_128436645.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.8e-79, bitscore:
                     260.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRARGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTSAKRAFLESFQNIVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARK
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALETYWADGSAMEKQTRKRGEQIEQAL
                     ISITEENLADVKEYRGRGLVWGLEFHDKERAGRVAHRAFELGLLIETSGPESEVVKLLP
                     ALTITPEELDEGLSVLARSVRETI"
     aSDomain        3785..4798
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EHO50_RS39340_Aminotran_3.1"
                     /evalue="1.90E-74"
                     /label="EHO50_RS39340_Aminotran_3.1"
                     /locus_tag="EHO50_RS39340"
                     /protein_end="366"
                     /protein_start="28"
                     /score="242.7"
                     /tool="antismash"
                     /translation="FDRARGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTSAKRAFLESFQNIVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARKEWLRALAELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALETYWADGSAMEKQTRKRGEQIEQALISITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="EHO50_RS39345"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="EHO50_RS39345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_128436646.1"
                     /sec_met_domain="ectoine_synt (E-value: 7.6e-56, bitscore:
                     180.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGEAELTDHETGRTHTITPGTMYLLDGHERHTLRIKEDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5413..6315
                     /gene="thpD"
                     /locus_tag="EHO50_RS39350"
     CDS             5413..6315
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="EHO50_RS39350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_128436647.1"
                     /sec_met_domain="PhyH (E-value: 2.2e-42, bitscore: 139.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAMTANVTDLYPSRAATEVSTPRQDPVVWGSPDTPGPIATTDL
                     QSFERDGFLAIDQLITEDEVAVYAQELERLVTDPAIRADERSIIEPKSKEIRSVFEVHK
                     ISEVFASLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGL
                     PNMRTVSVSIALTENYDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDE
                     ALTKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPF
                     AAPIRRPEFIGARDFTPVK"
     gene            complement(6337..7338)
                     /locus_tag="EHO50_RS39355"
     CDS             complement(6337..7338)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477735.1"
                     /locus_tag="EHO50_RS39355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_128436648.1"
                     /transl_table=11
                     /translation="MTDDHEAVRELLAAWAFGALDPADEKTVPPHLAECESCAAEAELL
                     RETVRLLEGPPRNAPAAPDEVLLSLALRARSAAPRVAEHVAPYSAAVAGLKALLPELEG
                     HWTTPVVHDWDVHATVAHLLAADEHLASLLGVGARVPPSRIAQGTRWEDAWNRRTADVI
                     AHEHGRTPEETVADWSAQAAELLGTPEARDAQLAARAVTLMGLRLPVADHFLVRGFETW
                     IHTDDIGRALGIAVPPPPQEHLHQLIRLAVRVLGLALGTTAPPVLFSVTGGEEWVLGSA
                     DEPVRAELAVDPVDFCLLVGGRYAPEAVPRGATGDAAAVRNVLETAASLAWL"
     gene            complement(7391..>7552)
                     /locus_tag="EHO50_RS39360"
                     /pseudo=""
     CDS             complement(7391..>7552)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477737.1"
                     /locus_tag="EHO50_RS39360"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VVHLAYVAGRTYRQAAVELGIPEGTAKTRLRTALRTLAETLADPP
                     DPALERGA"
     gene            complement(8019..9071)
                     /locus_tag="EHO50_RS39365"
     CDS             complement(8019..9071)
                     /NRPS_PKS="Domain: Aminotran_5 (16-344). E-value: 7.4e-24.
                     Score: 76.3. Matches aSDomain:
                     nrpspksdomains_EHO50_RS39365_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002242.1"
                     /locus_tag="EHO50_RS39365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_128436649.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.1e-19, bitscore:
                     63.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFAPKNVYLNTASSALLPARTVTALHRAVRMRAEG
                     TPLGPLYEDVEVVRASFARLAGVPVERVATGASVAEYGGLIAASLPAGAEVLTAEADFA
                     SLVNPFHMRGDLKVRAVPLDRLAESVRPDTALVAVSAAQSADGRIADLPAVRAAALAQG
                     ARTYVDASQSAGWLPLEAGAYDFVAAVGFKWLLGPHGVAFCVVPEDFGGLTPLLAGWVA
                     GEAPWDSCYGPVEDLAHSARRFDISPSLFTYAGLRTSLELIEELGVSAIQAHDLDLADR
                     FRAGLATLGHEPVPSPGSAIVSVPGLGHRQPELSAAGIEVANRAGNLRAAFHLYNTPAD
                     VDRLLDVLSA"
     aSDomain        complement(8040..9023)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EHO50_RS39365_Aminotran_5.1"
                     /evalue="7.40E-24"
                     /label="EHO50_RS39365_Aminotran_5.1"
                     /locus_tag="EHO50_RS39365"
                     /protein_end="344"
                     /protein_start="16"
                     /score="76.3"
                     /tool="antismash"
                     /translation="VYLNTASSALLPARTVTALHRAVRMRAEGTPLGPLYEDVEVVRAS
                     FARLAGVPVERVATGASVAEYGGLIAASLPAGAEVLTAEADFASLVNPFHMRGDLKVRA
                     VPLDRLAESVRPDTALVAVSAAQSADGRIADLPAVRAAALAQGARTYVDASQSAGWLPL
                     EAGAYDFVAAVGFKWLLGPHGVAFCVVPEDFGGLTPLLAGWVAGEAPWDSCYGPVEDLA
                     HSARRFDISPSLFTYAGLRTSLELIEELGVSAIQAHDLDLADRFRAGLATLGHEPVPSP
                     GSAIVSVPGLGHRQPELSAAGIEVANRAGNLRAAFHLYNTPADVDRL"
     gene            complement(9179..9895)
                     /locus_tag="EHO50_RS39370"
     CDS             complement(9179..9895)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140791.1"
                     /locus_tag="EHO50_RS39370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_128436650.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALEAFPHRDQVEVVHRSFELDP
                     GRAKGDIQPVITMLTRKYGMSAAQAQAGEENLGAQAAAEGLDYRTEGRDHGNTFDMHRL
                     LHLAKEHGRQDELIQILYRANFAEERSVFSEGDERLVELAVAAGLDADDVRKVLADPDA
                     YADDVRADEREAAQLGATGVPFFVLDRKFGVSGAQPAEVFQQALTQAWGERSPLTLIEQ
                     GDADACGPDGCAVPQH"
ORIGIN
        1 cagccagccc tggacgaccg ggtagtcgat gacgaccgcc tgcacctggc cggtgcgcag
       61 gccgttgatg acggcgtcgg aggactcgaa ggagacgggg tccaggccct tgctcttggc
      121 gtagtcctcg ccggtcgtct gtgcctgggc gccgaccttc ttgcccttga ggtccgcgaa
      181 ggaggagatg ccgctcttct tgtcgacgag gacggcctgg gtggcctcga agtacgggtc
      241 cgagaagtcg acgttcttct tgcgctcctc ggtgatggtc atgccggcgg cggcgaggtc
      301 gcactgcccg gcgttgaggg agccgccggt cttgaagttc tcgaagggct ggtcgacgat
      361 cgtctgcttc acgcccaggt cgtccgcgac caggtcgatg agggagacgt cgaagccctg
      421 caccttgccg tcgatctccg actggaacgg cggatacggc aggtgggtgc aggtggtgag
      481 ccggcccgcc ttgacgactt cgacaccgcc ggccgccgtc ttcgtaccgc tgccgccgtt
      541 gtcgtcggag gagcagccgg ccacgagcac cagcccggcc gtcgcggtgg tggcggccag
      601 aatgcgggtc cggcgcccga gggtcgtctt cacggggagc ctcctgtcag ggaactgtgg
      661 gttccgatta taaggagagg tttgagttcc tcaaaccaaa ccgatggtta aggggccgtt
      721 cgacctaaga agtcgggtgc ggggaggcgg ttgggagggg atggggaggg acggaagtgg
      781 gcgggaaggg gggcgagaag gggtgtgggg cgcccggtca ctgcccagtc accgcccggt
      841 ttacgctcgt cctcgtcgat ccggagcgaa gagagcgaag agagcccgcc gtgacccatc
      901 ccttcctgga cctgaccccg ctgagcgccg gccgattcgc cgccatcgag gaccgggtgg
      961 cgcggctgct cgggaccgag caggacgtcg tgatcatgca gggcgaggcg ctgctgccgc
     1021 tggaaggggc gatccggggg acggccgggc ccggcacggt cgcgctgaac gtgatcaccg
     1081 gtccgtacgg gcagaccttc ggggactggc tgcgtgactg cggtgcgacc gtgatcgatc
     1141 tggcggtgcc cttccatacg gccgtcacgg cggaccaggt ccgggaggcc ttcgccgagc
     1201 acccggagat cgacttcgtg tccctggtgc atgcggaggc ggcgaccggc aacaccaacc
     1261 ccgtcgcgga gatcggtgag gtcgtgcggg cgcacggggc gctgttctac ctggacgccg
     1321 tggcgtcgat cggggccgag ccggtgctgc cggacgcatg gggcgtggac ctgtgcgtca
     1381 tcggggcgca gaaggcgatg ggcgggccgg ccggggtttc ggcggtgtcg gtgagcgagc
     1441 gggcgtgggt gcggatggcg gcgaacccca gggcgccgcg gcggtcgtat ctgtcgctgc
     1501 tcgactggaa ggagcggtgg atcgacggtg ggcggaaggc gctgctgcat gcgcctgcgc
     1561 agctggagat gctggcgctc gaggcttgtg tggagcggat cgagggggac gggctggatg
     1621 cggtgatggc ccggcaccgg gccgccgcgc tgtcgactcg ggccggggcg gttgcgctgg
     1681 gtgggggctt ggagccgtat gtgtacgacg ccggggaggc cgctcctgtc gctacgacgc
     1741 ttcgggcgcc ggcgggggtg gtggcgtcgg aggtggtggc tcgggcgctg gcgggcgacc
     1801 ctgcgttgcc gctggccgcg gggggtgggg cgttggccaa ggagatgatt cgggtcaatc
     1861 actacggggt ggatgcggcg tcgggtgcgg tgcgggggtg tgtggcggcg ttgggggctg
     1921 cgctggcgga gaaggggctg ggtgtggatg tggagggggc gttgcgggtg gtggaggaag
     1981 cctggcggta gtcgggggct ggggcctggc tccttcgccc ccgccgcccc tacccgtccc
     2041 gtccttgaag gggctccgcc ccttcgaccc cgccaggggg ctttgccccc tggacccccg
     2101 tcggcctgaa cggcctcgtc ctcaaacgcc ggacgggctg aaatgcttgg accggcgctg
     2161 ggaagtgacc gccggacggg ctggatgatg ccggccggcg ccgggaagtg accgccgccg
     2221 cagtgaggcc gcacccccga gattcgcccc gcgctgaaag ccgtaccgcc ggtccgtccg
     2281 ccgtgccacg ctcccggcat gaccaccaac accccggaac tcctccccga cggccgcccc
     2341 ctccccctcc tgaccggcga cgagctcccc atgctcgaaa gctggctcgc cttccatcgc
     2401 gccaccctgg agctcaagtg ctcggggctg gacgacgtgc agctgcggct tgcctcggtc
     2461 gagtcgtcgg agttgacgtt gctcgggctg gtgcagcacc tcgcggagtg tgagcggaac
     2521 tggttccagc gggtggtggg tgggctcgac gtgccgccgg tgtacgggga ggggaacgag
     2581 agcgggtacg ccctcgatgc cgggcgtggg atcggggagg cgctcgcggt gtggcggcgg
     2641 gagatcgcgc gggggcggga ggtgtgtgcc gggcgggcgc tggagagtgt ggggcggatc
     2701 ggggacgggc cgatggccgg ggtcgaggtc agtctgcggt gggtgctcat tcacatgatc
     2761 gaggagtacg cacggcacaa cggccacgcc gacattctgc gggagcgcat cgacggagtt
     2821 accggagcct gaattcggtg taattctcga attcctttcc agggcagctt cccattctct
     2881 tctgcccgct ttcgccggac ctaaacgcaa agattccgcg aacaccaact ggaggaattc
     2941 gggcagatct cgtgggagtt acatggctgt gacgcgttcc acatcgcgcc ccttttgtgc
     3001 ggtattaacc ggacagagcg gtgcgatcca cccctgatcc gcgccacacc tcacgcccgc
     3061 gtgataacac acccggacct cacacgtaac ccctcccggc catgcaattt cgaatttccc
     3121 tcggtaaatt caattcgcat gactgccgca caagcagacc tgcaaatcga ccgtccggcg
     3181 gtggccgacg gggccgcgct gtggcggatt gcccgggact ccaaggccct cgacctcaac
     3241 tcgtcgtaca gctatctgct gtggtgccgc gacttcgccg acacctcggc cgtcgcccgc
     3301 gacgagcgcg gggagccggt cggcttcgtc accgggtacg tacggccgga tcgtccgcag
     3361 accctgctcg tctggcaggt ggccgtggac gacacgcacc gcggacgcgg gatcgccgcc
     3421 gcgctgctcg acgggctcgt cgcgcgagcc ggtcgcgagt acggcgtcac gagcgtcgag
     3481 accaccatca ccccgggcaa caccgcctcc gagcggctgt tcggcgcctt cgcggagcgg
     3541 cacggtgccg ccctggagcg cgaggtgctg ttcgatgccg gcctgttccc cgacgggccg
     3601 cacgaccccg aagtcctcta ccgcatcggt cccctctccc cctgacgacc agctgacgac
     3661 cagcacgcac tccccccacg caaacgagga gcgattcgtc gtgaccatca cccagcccga
     3721 cctcagcgtc ttcgagaccc tggagtccga ggtacgcagc tactgccgcg gctggcccac
     3781 cgtcttcgac cgcgcgcgcg gcagccgcat gtacgacgag gacggccacg agtatctgga
     3841 cttcttcgcc ggggccggct ccctgaacta cgggcacaac aaccccgtcc tgaaacgggc
     3901 gttgatcgac tacctggagc gggacggtgt cacgcacggg ctcgacatgt ccaccagcgc
     3961 caagcgcgcc ttcctggagt ccttccagaa catcgtgctg cggccgcgtg acctgccgta
     4021 caaggtcatg ttcccggggc ccacgggtac gaacgccgtc gagtcggcgc tgaagctggc
     4081 tcggaaggtg aaggggcgcg aggccatcgt gtccttcacc aatgccttcc acgggatgtc
     4141 cctggggtcg ctcgccgtca ccggcaacgc cttcaagcgg gccggcgccg ggatcccgct
     4201 cgtgcacggc acgccgatgc cgttcgacaa ctacttcgac ggcacggtcg aggacttcct
     4261 ctggttcgag cgcctgctcg aggaccaggg gtccgggctc aacaagcccg ccgccgtgat
     4321 cgtggagacc gtgcagggcg agggcggcat caacgtcgcc cgcaaggagt ggctgcgggc
     4381 gctcgccgag ctgtgcgagc ggcaggacat gctgctcatc gtcgacgaca tccagatggg
     4441 gtgcgggcgg accggggcct tcttctcgtt cgaggaggcc gggatcacgc ccgacatcgt
     4501 caccgtgtcc aagtccatca gcggctacgg actgcccatg tccctgtgcc tgttcaagcc
     4561 cgagctggac atctgggagc cgggcgagca caacggcacc ttccgcggca acaaccccgc
     4621 cttcgtcacg gccacggccg ccctggagac gtactgggcg gacgggtccg cgatggagaa
     4681 gcagacccgt aagcgcgggg agcagatcga gcaggcgctc atctccatca ccgaggagaa
     4741 cctcgccgat gtgaaggagt accgcgggcg cgggctcgtg tggggcctgg agttccacga
     4801 caaggagcgc gccggacggg tcgcgcaccg agccttcgaa ctcgggctgc tcatcgagac
     4861 gtcgggcccc gagagcgagg tcgtgaagct gcttccggcg ctcacgatca ccccggagga
     4921 gctggacgag gggctcagcg tcctcgcccg ctccgtgcgg gaaaccatct gaaacaccac
     4981 ctaggaggca tcgcagcacc gtgatcgtcc gttcgttcaa ggacatcgaa ggaaccgacc
     5041 gccatgtgaa atcggcgtcc ggcacctggg agagcaagcg catcgtcctc gccaaggaga
     5101 aggtcggctt ctccctgcac gagacgatcc tgtacgcggg tacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgcg tcgagggcga ggccgagctg accgaccacg
     5221 agaccgggcg gacgcacacc atcacgcccg ggaccatgta cctcctggac gggcacgaga
     5281 ggcacacgct gcggatcaag gaggacttcc gctgcctctg cgtcttcaac ccgcccgtga
     5341 ccggccggga ggaccacgac gagaacggcg tgtacccgct gctcaccgag cccgaggagg
     5401 tgtgagaaac ccatgaccac cgccatgacc gcaaacgtca ccgatctcta ccccagccgc
     5461 gccgccaccg aggtgtcgac gccgcgccag gaccccgtcg tctggggctc ccccgacacg
     5521 cccggcccga tcgcgacgac cgacctgcag tccttcgagc gcgacggctt cctcgccatc
     5581 gaccagctca tcaccgagga cgaggtcgcg gtctacgcac aggagttgga gcggctggtc
     5641 accgacccgg cgatccgtgc ggacgagcgc tcgatcatcg agccgaagtc caaggagatc
     5701 cggtccgtct tcgaagtgca caagatcagt gaggtgttcg cctctctggt gcgcgacgag
     5761 cgggtcgtcg gccgggcccg gcagatcctc ggctcggacg tgtacgtcca ccagtcgcgg
     5821 atcaacgtca agccgggctt cggggccagt ggcttctact ggcactccga cttcgagacc
     5881 tggcacgccg aggacggcct gcccaacatg cgtacggtgt ccgtctcgat cgcgctgacc
     5941 gagaactacg acaccaacgg cgggctcatg atcatgccgg gctcgcaccg cacgttcctg
     6001 ggctgtgcgg gtgccacgcc gaaggacaac tacaaaaagt ccctgcagat gcaggacgcg
     6061 ggcacgccgt ccgacgaggc gctgaccaag atggcctccg agtacggcat caagctcttc
     6121 acgggcaagg ccggttcggc tacctggttc gactgcaact gcatgcacgg ctccggcgac
     6181 aacatcacgc cgttcccgcg cagcaacgtg ttcatcgtgt tcaacagcgt ggagaacgcg
     6241 gccgtcgagc ccttcgcggc tccgatacgg cggccggagt tcatcggcgc gagggacttc
     6301 acccccgtga agtgacgctc acacgtgaag tgatcctcac agccacgcca gcgacgcggc
     6361 cgtctccagt acgttccgta cggcggccgc gtcgcccgtg gcacccctcg gcaccgcctc
     6421 cggggcgtat cgtccgccga ccagcaggca gaagtcgacc ggatcgacgg cgagttcggc
     6481 ccgcacgggc tcgtccgccg accccagcac ccactcctcg cccccggtga ccgagaagag
     6541 caccgggggc gcggtggttc ccagagccag gccgaggacg cgtacggcga ggcggatcag
     6601 ctggtgaagg tgctcctggg gcgggggcgg gacggcgatg ccgagtgcgc ggccgatgtc
     6661 gtcggtgtgg atccacgtct cgaagccgcg caccaggaag tggtcggcga cgggcagccg
     6721 cagccccatc aacgtcacgg cccgggcggc cagttgggca tcgcgggcct ccggcgtgcc
     6781 gagcagctcg gccgcctgcg cggaccagtc ggccacggtc tcctcgggcg tacggccgtg
     6841 ctcgtgcgcg atgacatcgg cggtacggcg gttccaggcg tcctcccagc gggtgccctg
     6901 cgcgatccgc gagggcggca cccgggcgcc gacgccgagc agggaggcca ggtgctcgtc
     6961 ggcggcgagg aggtgggcga cggtggcgtg cacgtcccag tcgtgcacga ccggcgtggt
     7021 ccagtggcct tccagctcgg ggagcagtgc cttcaggccg gcgaccgcgg cggagtaggg
     7081 agcgacgtgc tcggcgacgc gcggggcggc ggagcgggcg cgcagggcga gggacaacaa
     7141 gacctcgtcc ggcgccgcgg gagcgttccg cggcggcccc tccagcagtc gtaccgtctc
     7201 ccgcagcagt tccgcctccg ccgcgcagct ctcgcactcg gccaggtgcg gcgggaccgt
     7261 cttctcgtcg gccgggtcga gggcgccgaa ggcccaggcg gcaagcagct cccgtaccgc
     7321 ctcgtgatcg tcggtcaccg cgcgcccacc tcctgcccgt gcctgtcccc ggttcgtacc
     7381 cctctcgcca tcatgcgcct ctttcgagcg ccgggtccgg tgggtcggcc aaagtctccg
     7441 ccaaggtacg cagggcggtg cgcagccggg tcttggccgt gccctcgggt atccccagct
     7501 ccacggcggc ctgccggtag gtgcggcccg cgacgtaggc gaggtggacg acgacctcgg
     7561 cggtggccgg gttgcgggtg ccccttgtag cgccccacaa caccggcctg ccgcgcgccc
     7621 gccgatcggt gggcgattgc catggggcga acggccccgc tgccggtctg ctccaccggc
     7681 accaccaccg ttcctgggaa ccgaccggga aagctccgat cagccgcacg ccccgaacgg
     7741 tcttgggcgc actggtcatc cgctttcaca tcccgggcgg cgacacggat cacgtccgtg
     7801 gtccgagccg ggcgggcgga gtccctcacg atctggccat gtgggtgcgg ccagaggcga
     7861 cggggaggcc ctgaaccatc tcgctggtgg agcaggggcc ccgcacgctg acgccacacc
     7921 cggcgtccca gattgcgcga cgacactacc tcggccctct cagcacagcg caagcccacg
     7981 agccgacgat cacacgatcg tggtaagtca ccgcaggttc aggccgagag gacgtccagc
     8041 aaccggtcga catccgcggg ggtgttgtag aggtggaagg ccgcgcgcag gttgcccgcg
     8101 cggttggcga cctcgattcc ggccgcgctc agctcgggct ggcggtggcc gagtccgggc
     8161 acggagacga tcgccgagcc gggcgagggc accggttcgt ggccgagggt cgcaaggccc
     8221 gcgcggaagc ggtcggcgag gtcgaggtca tgggcctgga tggcggacac gccgagttcc
     8281 tcgatcagct ccagggacgt gcgcagcccg gcgtaggtga agaggctggg gctgatgtcg
     8341 aagcgccgag cggagtgggc gaggtcctcg acggggccgt agcagctgtc ccagggggcc
     8401 tctccggcga cccacccggc gagcagcggc gtcagcccgc cgaagtcctc cgggacgacg
     8461 cagaacgcga cgccgtgcgg gcccaggagc cacttgaagc cgacggcggc gacgaaatcg
     8521 tacgcgccgg cctccagcgg cagccatccg gccgactggg aggcgtcgac gtaggtgcgc
     8581 gccccctgcg ccagtgccgc cgcgcgcacg gcgggcaggt cggcgatccg gccgtcggcg
     8641 gactgcgcgg cgctgaccgc gacgagtgcg gtgtccgggc ggacggactc ggcgaggcgg
     8701 tccaacggga cggcgcgcac cttgaggtcg ccgcgcatgt ggaaggggtt cacgagggag
     8761 gcgaagtcgg cctccgcggt gaggacttcg gcacctgcgg ggagcgaggc cgcgatcagc
     8821 ccgccgtact cggcgaccga ggccccggtc gcgacccgct cgaccgggac gccggccagc
     8881 cgggcgaagg aggcgcgcac gacctccacg tcctcgtaca gagggccgag cggcgtgccc
     8941 tcggcccgca tccgcacggc ccggtgcagg gcggtgacgg tgcgggccgg cagcagggcg
     9001 ctgctcgcgg tgttgagata gacgttcttc ggtgcgaact cggcgcggac caggctctcg
     9061 aaggtctcca tggctccact gtgggccctg aacacctatc ggtccattgc gaatctttac
     9121 gtggtttcgc taaggaacgc ttatagatcg gccccgacct gcgggttcgc tcagcagctc
     9181 agtgctgcgg caccgcgcag ccatccggac cgcacgcgtc cgcgtcgccc tgctcgatca
     9241 gcgtgagcgg cgaccgctcg ccccacgcct gcgtcagcgc ctgctggaag acctcggcgg
     9301 gctgggcgcc ggagacgccg aacttgcggt cgaggacgaa gaacggcacg cccgtggcgc
     9361 cgagctgggc ggcctcgcgc tcgtcggcgc gcacgtcgtc ggcgtaggcg tccgggtcgg
     9421 cgaggacctt gcgcacgtcg tcggcgtcga gtccggccgc gacggccagc tccacgagcc
     9481 gctcgtcgcc ctcggagaag acggaccgct cctcggcgaa gttcgcccgg tagaggatct
     9541 ggatcagctc gtcctgcctg ccgtgctcct tggcgaggtg caggaggcgg tgcatgtcga
     9601 aggtgttgcc gtggtcgcgg ccttcggtgc ggtagtcgag tccctccgcg gcggcctgcg
     9661 cgccgaggtt ctcctcgccg gcctgggcct gggccgcgct catgccgtac ttcctggtga
     9721 gcatggtgat cacgggctgg atgtcgccct tggcgcgccc ggggtccagc tcgaacgagc
     9781 ggtgcaccac ctcgacctgg tcgcggtgcg ggaaggcctc gagcgccttc tcgaagcggg
     9841 ccttgcccac gtagcaccag gggcaggcga tgtcgctcca gatctcgacg cgcatgtgtg
     9901 tggctctctc cagtcgtacg ggtacgggga ctcctccggt tagtgcgtga acgttcaatc
     9961 agcggggttc attccctggg cacggtgaag cacaggttca ggtggtggtc cccgtcggcg
    10021 ggcgggttct gcgggtccgg acgccagccc cggccggcgt agaagatctg cgcacgccgg
    10081 ttgtccacat gcacgtcgag cacggccgtg cgcttcccgt ccgcccgcca ctgctccaca
    10141 caggcggcat gcagggcggt accgacgcca cgaccccacc agtcggggtc gacgtggaac
    10201 tggaacagct tgaccgtgtc ggcaggggtg tcctccgggg tgcggaagga ggcgagaccg
    10261 acgatccggc tctgctcgac gacgcacagc acatgcccgt cgggccgctc gatggcggtg
    10321 cgccaggcgg cggaccagtc ggtgccgtcg tccgggatcc cgtccgggta gtacgtcgcg
    10381 cgggcgcgcg cgtgcagggc ggcga
//
