LOCUS       NZ_RQJW01000004        11629 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces cyaneus strain CGMCC 4.1671 Scaffold3_1, whole genome
            shotgun sequence.
ACCESSION   NZ_RQJW01000004
VERSION     NZ_RQJW01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces cyaneus
  ORGANISM  Streptomyces cyaneus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 732681)
  AUTHORS   Fang,B.-Z., Han,M.-X., Jiao,J.-Y., Xie,Y.-G., Zhang,X.-T., Liu,L.,
            Zhang,Z.-T., Xiao,M. and Li,W.-J.
  TITLE     Streptomyces sp. nov., a novel actinobacterium isolated from a karst
            cave sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 732681)
  AUTHORS   Fang,B.-Z., Han,M.-X., Jiao,J.-Y., Xie,Y.-G., Zhang,X.-T., Liu,L.,
            Zhang,Z.-T., Xiao,M. and Li,W.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2018) School of Life Sciences, Sun Yat-sen
            University, No. 135 Xingang West Road, Guangzhou, Guangdong 510275,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-OCT-2018
            Assembly Method                   :: SPAdes v. 3.6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004028635.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 03:28:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,717
            CDSs (total)                      :: 8,635
            Genes (coding)                    :: 8,413
            CDSs (with protein)               :: 8,413
            Genes (RNA)                       :: 82
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 77 of 222
            Pseudo Genes (incomplete)         :: 187 of 222
            Pseudo Genes (internal stop)      :: 12 of 222
            Pseudo Genes (multiple problems)  :: 50 of 222
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:58:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 470723
            Orig. end                         :: 482352
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RQJW01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11629
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11629
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11629
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6629](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6629)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(332..345)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.74"
                     /tool="antismash"
     gene            424..1155
                     /gene="hemQ"
                     /locus_tag="EHO50_RS17410"
     CDS             424..1155
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="EHO50_RS17410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_128431056.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADVMIWWHAETSDQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWLLAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFFTGRRKSVAELVAGLA"
     gene            complement(1195..2865)
                     /locus_tag="EHO50_RS17415"
     CDS             complement(1195..2865)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (109-483). E-value: 3e-21.
                     Score: 68.1. Matches aSDomain:
                     nrpspksdomains_EHO50_RS17415_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997029.1"
                     /locus_tag="EHO50_RS17415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_128431057.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-24, bitscore: 82.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAPALYSAAGALLLTPLAAAPAGSTGGAPGADEWHGTTVAVARA
                     EVSGLTFGSCPKERELPDNVQCGTVSVPLDYAHPDGKQIKLTVSRMRATHKDPHNSKHR
                     VPRQGALVYNPGGPGASSLHFPLIGVLPAWKRLAGAYDLVGYAPRGVGPSAPLSCQDPK
                     HSLKGPTHAPTHPSESYKKERIARAKAYARGCAQRSGSALRHYNSLNNARDLDVLRAAL
                     GEEKLTFMGASYGTYFGAVYATLFPSHVRRMVFDSAVNPDPAQVWYRNNLAQSAAFEGR
                     WEDFREWVAKHHDVYGLGDTPEEVLRSYDKARARLAAEPAGGKIGTGQLQEAFLQAGYY
                     DDYWPARAQALSAYLKGDPKPLVELAAPVTEAAAEAENSSAVYTAVECNDAPWPTDWAV
                     WDRDNTRLARVAPFETWDNVWMNLPCAYWQAPRQAPIDVRTGPGEVPPILILAAERDAA
                     TPYEGALEMHRRLAGSVLVTERDAGTHGIAYGPNACVNGHLDAYLLEGRLPARRASCEG
                     HPEPKPERPGDPQAKKLRQQTETNPGGRPQTAATARLPH"
     aSDomain        complement(1417..2538)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EHO50_RS17415_Abhydrolase_1.1"
                     /evalue="3.00E-21"
                     /label="EHO50_RS17415_Abhydrolase_1.1"
                     /locus_tag="EHO50_RS17415"
                     /protein_end="483"
                     /protein_start="109"
                     /score="68.1"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASSLHFPLIGVLPAWKRLAGAYDLVGYAPRGVGPS
                     APLSCQDPKHSLKGPTHAPTHPSESYKKERIARAKAYARGCAQRSGSALRHYNSLNNAR
                     DLDVLRAALGEEKLTFMGASYGTYFGAVYATLFPSHVRRMVFDSAVNPDPAQVWYRNNL
                     AQSAAFEGRWEDFREWVAKHHDVYGLGDTPEEVLRSYDKARARLAAEPAGGKIGTGQLQ
                     EAFLQAGYYDDYWPARAQALSAYLKGDPKPLVELAAPVTEAAAEAENSSAVYTAVECND
                     APWPTDWAVWDRDNTRLARVAPFETWDNVWMNLPCAYWQAPRQAPIDVRTGPGEVPPIL
                     ILAAERDAATPYEGALEMHRRLAGSVLVTERDAG"
     gene            complement(2989..3786)
                     /locus_tag="EHO50_RS17420"
     CDS             complement(2989..3786)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122142.1"
                     /locus_tag="EHO50_RS17420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_128431058.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAVAGTACTRLCLAAVHAAAMETNEATAGQGRDLT
                     LYEAAFLSGGPRRVADLTLVSMARQRRLLLARTGWATVVDPRGRDEMERSVIGAIGPQG
                     QSRIEPVRDAAAAADAVGSLADRLVSAGLAVPDGTRSGIAAAIRQVQLAAVVVLALGAV
                     ALLAPAQSDMPAHLVALWFALPFVLTLSCLAIARVEIHPYAQWASPAGQRLLSGLVRRS
                     RGGGDRTFLTSVAVRGVRAIGEPDLRAAFAHREPHHEAGHWND"
     misc_feature    3787..3800
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     18.25"
                     /tool="antismash"
     gene            complement(3884..4678)
                     /locus_tag="EHO50_RS17425"
     CDS             complement(3884..4678)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657154.1"
                     /locus_tag="EHO50_RS17425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_128431059.1"
                     /transl_table=11
                     /translation="MRPRPRPPINGRGIFSGTGLIITGLTATLLAMIFPIWSYADRSGT
                     GLDVLNAETVSTGYGPLSALDRDFITKVRLAGLWELPAGRQAQQKGMTPAVRTAGQHLI
                     EGHAFLDERVRDVAAKLSLQLPNEPTEQQQEWLATLDSAQGVEYDRQFANILRLAHGKV
                     FSVVAQVRAGTRNSLVRKLADDANTTVLDHITVLEATGYVDFDALARDMVADSTPPLTP
                     APAPPGPTLDPAPAVPVTPPPTPTYTLPPAATSRPLENAGKH"
     misc_feature    complement(4681..4697)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.35"
                     /tool="antismash"
     misc_feature    4727..4743
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.02"
                     /tool="antismash"
     gene            complement(5001..6629)
                     /locus_tag="EHO50_RS17430"
     CDS             complement(5001..6629)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993751.1"
                     /locus_tag="EHO50_RS17430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_128431060.1"
                     /sec_met_domain="DUF692 (E-value: 1.6e-97, bitscore: 321.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRLGTGIGWRPEIADAVERMPGIDWVETVAENVCPGHLPESLVR
                     LGERGVTVIPHGVSLGLGGADRPDAGRLAALAERVEALGSPLVTEHIAFVRAGGPLTAS
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALISWPGEEMTEGQFLY
                     ELADRTGVGLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPEPVLDILTDLASRVSPPGVLLERDENFPEPAELERELGAIRGALEKGGAERGAA
                     VVGPYAEGPVAGRPSAEGPTAGGASPRRASAEVCRSEEASAEGAFGDGGSSTESSSTGA
                     SRGSAPGERISQEAREAESARKGASSTTDASGSSAPVEGTSQQAVRRPEAVREPEGAAS
                     GGAFADPARQRLALAQAALLSALVAGTPVPEGFDRVRLGVQARALAGKRADVVAKVAPE
                     LPVILGGGYRRAFLAYGHGHPMTDGYRRDALDFAGYLLAEGRLEDARVRAELREWWLER
                     SGPRPRSRRPGVRLARATRRVLLRR"
     gene            complement(6840..7556)
                     /locus_tag="EHO50_RS17435"
     CDS             complement(6840..7556)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133762.1"
                     /locus_tag="EHO50_RS17435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_246101202.1"
                     /transl_table=11
                     /translation="MTSDPSVPGESPFRTEPSTRDEAPQYVLPLVVRIERAAPPPRTDA
                     LETAARAVLVMLSDERSLGEGEWAEAVRDWQDARIRKVVRRARGAEWRRAEGLPGITVT
                     GKSAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPPVDADPSAPVLWLNPDLDMSAGK
                     AMAQAGHAAQLAWWELSDEERDAWRDAGFPLAVRTAQPGRWAELTTSVLPLVRDAGFTE
                     IAPGSCTVVAYHPALR"
     misc_feature    7576..7590
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.77"
                     /tool="antismash"
     gene            7612..8490
                     /locus_tag="EHO50_RS17440"
     CDS             7612..8490
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997024.1"
                     /locus_tag="EHO50_RS17440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_128431062.1"
                     /transl_table=11
                     /translation="MQRDLRLDNVGRRYGIRGPWVLRGVHLTVPPGTLIRIDGPNGTGK
                     STLLRLLARIDAPSEGRITGRPRTAYVPERFPTALPFTALGYLTHLGTVHGLSRPAAGR
                     AALEWLDRFGAAPYARMPMAQLSKGSSQKVAVVQALLAEPELLVLDEAWTGLDAAAREE
                     LERAVAERTAGGTAVVFVDHDPRRLAGAPDATYTVRDAGLDRRTDQAPSDPDGPHVIVV
                     VQGPRGGQLPTDVPRTVTSVEEPTPGTHRLTVPASHSDILLRTLLTARPPWHVVSVTQE
                     RPARPETESRR"
     misc_feature    7675..7677
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8505..9176
                     /locus_tag="EHO50_RS17445"
     CDS             8505..9176
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="EHO50_RS17445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter"
                     /protein_id="WP_128431275.1"
                     /transl_table=11
                     /translation="MNALLRYQAALLARSQRWLPPFILYAAFLGVGVQSGQPVLDSLGY
                     AAAALLPVAAWLVRICVTNEPPAARSCVSAAVGPGRAHLAALLVALAAAAALGTVATVF
                     VTLISNPVSADLQTRVPRWPAGAAGLLAALACALLGTAVGALTTWPLLRSTGRAVPTML
                     LAALLSVVVTGSPAQAAVSGLVTGSRSGTVPLPLLPLAAAALLTAAATAVACALTSRRS
                     P"
     gene            complement(9202..9393)
                     /locus_tag="EHO50_RS17450"
     CDS             complement(9202..9393)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125244.1"
                     /locus_tag="EHO50_RS17450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_128431063.1"
                     /transl_table=11
                     /translation="MSEEQPTTDYCLINATRPPKADGPPYAECVLCREPTEYPESYKGI
                     TLCPVCEWQEAQRTACSG"
     gene            complement(9456..10310)
                     /locus_tag="EHO50_RS17455"
     CDS             complement(9456..10310)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657146.1"
                     /locus_tag="EHO50_RS17455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_128431064.1"
                     /transl_table=11
                     /translation="MITLVRRVTAVCALGAALSALASCAPETTRAPLPAPSATAASSPV
                     AEPPTLAPGPAGLTPVFEHGPRTRDKAVALTFDADMTSDQGPRAASGERFDNPGLIAAL
                     RELKVPATVFMTGRWAEEYPVQARAIGRDPLFEVANHSYSHHAFTDDCYGLPTVDEQRM
                     RTDVERAYASFRKVGVRDAMPYFRFPGGCYDRRALKALTPVGVTAVQWDVVSGDAFATD
                     ARSVAREVLDGVRAGSVVVLHCTLSAAPTTERVVRSVVPELRGKGFRFVKVSELIGAAG
                     GRR"
ORIGIN
        1 gtggggctgg atcgccgacg agaacccgga tgtggtcgtc ctgcgcacgt ccgtggggcg
       61 gtacggcgag acggagatcc ttcagcgcgg cgacgaccac ctcgtggagg tctcccgccg
      121 cgacctcagg gaagccaccg gcctcgacgc cgcccccctc gaaacccgcg tcacccgctg
      181 gaccgacggc ctgccccagt accccgtcgg ccaccacgcg cgcgtggccc gcatccgcga
      241 gcacgtcgcc aaggtgccgg gcctcgccgt gtgcggcgcg cagtacgacg gcgtgggcat
      301 cccggcgtgc atcgcgagcg cgtacgccgc ggtggaccag atccacggtg acctgagccg
      361 tgtggaggag ctcaccgcca acccggtgca gagtctgcac ggaggagcgg gagaataagg
      421 gacatgagtg acgacgcccc caccaccgag tccggcagga tcccgaacaa gggcaagctg
      481 gccaaggacc tcaacgaggt catccgctac accctctggt ccgtcttcaa gctgaaggac
      541 gtgctccccg aggaccgcgc gggctacgcc gacgaggtcc aggagctgtt cgaccagctc
      601 gccgccaagg acgtgacgat ccgcggcacc tacgacgtct ccggcctgcg cgccgacgcc
      661 gatgtcatga tctggtggca cgccgagacc agcgatcagc tgcaggaggc gtacaacctc
      721 ttccgccgca ccaagctggg ccgtgccctg gagccggtct ggtcgaacat ggcgctgcac
      781 cgccccgccg agttcaaccg ctcgcacatc ccggcgttcc tcgccgacga gacgccgcgc
      841 aactacgtca gcgtctaccc cttcgtgcgt tcctacgact ggtacctgct gcccgacgag
      901 gaccgccgcc gcatgctcgc cgaccacggc aagatggccc gcggctaccc cgacgtgcgc
      961 gccaacacgg tcgcctcgtt ctcgctgggc gactacgagt ggctcctggc cttcgaggcc
     1021 gacgagctct accgcatcgt cgacctcatg cgccacctgc gggcttccga ggcccggatg
     1081 cacgtccgcg aagaggtgcc gttcttcacg gggcgccgga agtcggtggc ggagctggtg
     1141 gccgggctgg cctgaccgga cgcggtgctg cgagggcggg ccggggtcgc ctgatcagtg
     1201 cggtagccgg gcggttgcgg cagtctgggg tcggccgccc gggttggtct cggtctgctg
     1261 gcgcagcttc ttcgcctggg ggtcgcccgg gcgctccggc ttcggctcgg ggtgcccttc
     1321 gcacgaggcg cgtcgcgccg ggaggcggcc ctccagcagg taggcgtcga ggtggccgtt
     1381 gacgcaggcg ttcgggccgt acgcgatgcc gtgcgtaccg gcgtcccgtt cggtcaccag
     1441 gaccgagccc gccagacgcc ggtgcatctc cagtgcccct tcgtacggcg tggccgcgtc
     1501 ccgctcggcg gccaggatca gaatcggtgg cacctcaccc ggccccgtcc gcacgtcgat
     1561 gggcgcctgc cggggtgcct gccagtaggc acacggcagg ttcatccaca cgttgtccca
     1621 ggtctcgaac ggcgccacgc gcgcgagccg cgtgttgtcc cggtcccaga ccgcccagtc
     1681 cgtcggccag ggcgcgtcgt tgcactcgac ggccgtgtac accgcgctcg agttctcggc
     1741 ctcggccgcc gcctccgtca cgggtgcggc cagctcgacc agcggcttgg ggtcgccctt
     1801 cagatacgcc gacagcgcct gcgcccgcgc gggccagtag tcgtcgtagt acccggcctg
     1861 caggaacgcc tcctgcaact gcccggtccc gatcttcccg cccgccggct ccgcggccag
     1921 ccgcgcccgc gccttgtcgt aactgcgcag cacctcctcg ggggtgtcac cgagcccgta
     1981 gacgtcgtgg tgcttggcga cccattcccg gaagtcctcc caccgccctt cgaacgcagc
     2041 cgactgggcg aggttgttgc ggtaccagac ctgcgcgggg tcggggttca ccgcagagtc
     2101 gaacaccatc cgccgtacgt gggaggggaa gagagtcgcg tacaccgcgc cgaagtaggt
     2161 gccgtacgac gcgcccatga acgtcagctt ctcctcgccc agcgcggcgc gcaggacgtc
     2221 caggtcacgg gcgttgttga gggagttgta gtgccgcagg gcgctgcccg agcgctgggc
     2281 gcagccgcgg gcgtaggcct tcgcgcgggc gatgcgctcc ttcttgtacg actccgaggg
     2341 gtgcgtcggc gcgtgggtgg gccccttgag ggagtgcttg gggtcctggc aggacagcgg
     2401 cgcggaaggt cccactccgc gcggggcgta gccgacgagg tcgtacgcgc ccgccagccg
     2461 cttccacgcg ggcaggacac cgatcagcgg gaagtgcagg ctggaggcgc ccgggccgcc
     2521 cgggttgtag accagggcgc cctggcgcgg cacgcggtgc ttgctgttgt gcgggtcctt
     2581 gtgggtggcc cgcatccggc tgacggtgag cttgatctgc ttgccgtcgg gatgtgcgta
     2641 gtcgagcggg acggacaccg tgccgcactg cacgttgtcc ggcagctccc gctccttcgg
     2701 gcagctgccg aaggtgaggc cgctcacctc ggcgcgtgcc acggccaccg tcgtgccgtg
     2761 ccactcgtcc gcgcccgggg cgcccccggt gctgccggcc ggggcggcgg cgaggggcgt
     2821 caggagcagg gccccggcgg ccgagtagag ggcgggagct ctcatggcgt atcccttcgc
     2881 agcacggcag caacaaaagg gatatttcgt ttgtctgttg gtgaaggcaa gcaccacctg
     2941 ccggtgtcgg cgtcaatgcc ccctatgcgc caccgaaccc cgcaccggtc agtcgttcca
     3001 gtgccctgcc tcgtggtgcg gctcgcggtg cgcgaaggcc gcgcgcaggt ccggctcgcc
     3061 gatcgcgcgt acgccccgta cggcgacgga ggtgaggaac gtacggtcgc ccccgccccg
     3121 ggaacgccgg accaggccac tcagcaaccg ctgccccgcc ggggaggccc attgcgcgta
     3181 cgggtggatc tcgacccggg cgatggcgag gcagctcagg gtgagcacga aggggagcgc
     3241 gaaccacagg gcgaccagat gcgccggcat gtccgactgg gccggcgcca gcagggcgac
     3301 cgcccccagc gccaggacga ccaccgcggc gagctgcacc tgccggatcg cggccgcgat
     3361 gccgctgcgc gtcccgtcgg gtacggcgag gcccgcgctg accagccggt cggcgaggct
     3421 gccgaccgcg tcggcggcgg ccgcggcgtc ccgcaccggc tcgatccggg actggccctg
     3481 aggcccgatg gcccctatca ccgagcgctc catctcgtcc cggccccggg gatcgacgac
     3541 cgtcgcccag ccggtgcgcg cgagcagcag ccgccgctgc cgcgccatgg agacgagggt
     3601 cagatcggcc acccgccggg ggccgccgga caggaacgcg gcctcgtaca gcgtcagatc
     3661 gcggccctgg cccgcggtcg cctcattcgt ttccatggcc gcggcgtgca cggcggccag
     3721 gcacagccgg gtgcacgcgg tgccggcgac ggcccaggcc agcagcagga gaaggaccca
     3781 gaacatggca cccattttgc ggccaaaccg ccgccgctct cgcggagttg gttgctcgcc
     3841 gtgggggttt ctccattgat ggttgcggtt ttggggtggt ttatcagtgc ttaccggcat
     3901 tctcgagtgg gcgactggtg gcggccggcg gcagggtgta cgtcggtgtc ggcggcgggg
     3961 tcaccgggac ggccggagcg gggtcgagag tcgggccggg cggagcgggg gcaggggtga
     4021 gcggaggggt gctgtcggcg accatgtcgc gggccagggc gtcgaagtcg acgtaaccgg
     4081 tggcctccag gaccgtgatg tggtccagca ccgtggtgtt ggcgtcgtcg gccagcttgc
     4141 gcacgaggga gttgcgcgtg ccggcccgca cctgggcgac gacggagaac accttgccgt
     4201 gcgccagccg caggatgttg gcgaactgcc ggtcgtactc gacgccttgg gccgagtcca
     4261 gggtggccag ccactcctgc tgctgctcgg tcggctcgtt gggcaactga aggctgagct
     4321 tggcggcgac gtcacgcacc cgctcgtcca ggaacgcgtg cccttcgatc aggtgctgcc
     4381 ccgcggtccg tacggccggc gtcatgccct tctgctgagc ttgccggccg gcgggcagct
     4441 cccacagccc ggccagccgg accttcgtga tgaagtcgcg gtcgagggcg gacagcggcc
     4501 cgtacccggt cgagacggtc tcggcgttga gaacgtccag accggtcccg gaccggtcgg
     4561 cgtacgacca gatcgggaag atcatcgcca gcagcgtcgc cgtcaggccc gtgatgatga
     4621 gcccggtgcc gctgaagatg cctcggccgt tgatgggggg tcgcggtcgc ggtcgcatgg
     4681 tgcctcctgt tgcggcaccg cacgttagtg cgcttcgggg gttgggttgg catattactt
     4741 cggggtacct cgggggctac gccaactgcc gtaccggagc gaatggttgc aacgggggtg
     4801 gttacgggcg aataggggag gagacgtggg cggcgtacgg tgacaaaggc ggacgaagcg
     4861 ggcgggctga ccgtgttgtg agcgggggtg ttaaccccgg cacactcgcc ggggcggggg
     4921 cgtccagtgg gggagcggga gttggcgtag gcggggtccg ccgggctatg ggcgcagagc
     4981 tgatgggccg cgtagcccgc tcaccgccgc agcagcactc gccgtgtggc ccgggccagc
     5041 cgcacccccg gccgccgtga ccgcggcctt ggccccgacc gctccagcca ccactcccgc
     5101 aactccgcgc gtacccgagc gtcctcaagc cggccctcgg ccagcagata cccggcgaag
     5161 tccagcgcgt cgcgccgata gccgtcggtc atcgggtgcc cgtggccgta cgcgaggaac
     5221 gcccgccgat acccgccccc gagaatcacc ggcaactcgg gcgcgacctt ggccacgacg
     5281 tccgcccgct tcccggcgag cgcccgcgcc tgcacgccga gccgcacccg gtcgaacccc
     5341 tccggcacgg gcgtaccggc gacgagcgcg gacaacagcg cggcctgcgc gagagcgagg
     5401 cgctgccgag cggggtcggc gaacgccccg cccgacgccg ccccctccgg ctcacgcact
     5461 gcctccggcc tgcgcaccgc ctgctgcgag gtcccctcca cgggcgccga gcttccggag
     5521 gcgtccgtcg tcgacgacgc ccccttgcgt gccgactccg cctcccgcgc ctcctgagag
     5581 atcctctccc caggcgcgga accccttgaa gcccccgtcg acgagctctc cgttgacgac
     5641 cccccgtccc cgaatgctcc ttccgcagag gcctcttccg agcgacacac ctccgcggat
     5701 gccctcctcg gggacgctcc gcccgcagtc ggcccctccg cggacggcct tcccgcaacc
     5761 ggcccctccg cgtacggccc tacgacagcc gcccccctct cagcgcctcc cttctccaaa
     5821 gccccccgaa tcgcccccaa ctcccgctcc agctctgccg gttcggggaa gttctcgtct
     5881 cgctccagca ggacccccgg cggagacacg cgggaggcga ggtcggtcag gatgtcgagg
     5941 accggctccg ggacagggtg ggcgtggctg tcgtgccaga cgccgtcgcg ttcgaagccg
     6001 cccgcgacat ggacgtacgc gatggcctcc agcggaagtt cggcgagcgc cttggcgggg
     6061 tcctcgcccc ggttgacgtg gttggtgtgc aggttggcga cgtcgatgag gaggcctacg
     6121 cccgtgcggt cggcgagttc gtacaggaac tgcccctcgg tcatctcctc gcccggccag
     6181 gagatgagcg cggcgatgtt ctccacggcc agcggcaccg gcagcgcgtc ctgggcgata
     6241 cggacgttct cgcacagcac gtcgagggcg tcccgggtgc gggggaccgg caggaggtgt
     6301 cccgcctcca ggcgcgggga cgccgtcagt ggaccgcccg cccgtacgaa cgcgatgtgc
     6361 tcggtgacca gcggtgaccc cagtgcctcc acccgctcgg cgagggcggc cagccgcccg
     6421 gcgtcgggcc ggtccgcgcc gcccagcccg agcgagacgc cgtgcgggat cacagtcacc
     6481 ccgcgctccc ccagccgtac cagggactcc ggcagatgtc cggggcagac gttctcggcg
     6541 accgtctcga cccagtcgat gcccggcatt cgctccacgg cgtccgcgat ctccggacgc
     6601 catccgatcc ccgtgcccag tcgcttcatc gtcccctcct cggcctcttc cgtacccgct
     6661 gccccgtctc ctcggacgtg ccggacgtga cgggggtatg gccccgaagg tcccgaccga
     6721 acctcgcgga cggcaccttc agagcaacat ttgaggttgg ggcccacgtc tcgcaccccg
     6781 cgtacggcca ccacgaggtc gtacgcgcgg gacgccgggg atgttcaaaa gccggaggcc
     6841 taccgcagag ccgggtggta ggcgacgacc gtgcacgacc cgggcgcgat ctccgtgaac
     6901 cccgcgtccc gcaccaacgg cagcacactg gtcgtcagct cggcccagcg ccccggctgt
     6961 gcggtccgta cggcgagcgg gaagcccgcg tcgcgccagg cgtcccgctc ctcgtccgac
     7021 agctcccacc aggcgagctg cgccgcgtgc cccgcctgcg ccatcgcctt gccggccgac
     7081 atgtcgaggt cggggttcag ccagagcacg ggcgccgacg ggtcggcgtc cacgggcggc
     7141 tccggatcgt cgaggtcggt cccggacacc tggagcttcg ccagctcctt gggccacccg
     7201 tccaggggca cgggcgggaa cacccgcacc tcggccgact tgccggtgac agtgatgccc
     7261 ggcagccctt cggcccgccg ccattcggct ccgcgagccc gccgcaccac cttgcggata
     7321 cgggcgtcct gccagtcccg cacggcctcg gcccactcgc cttcgccgag ggaccgctcg
     7381 tcgctcagca tgaccagcac ggcgcgggcg gcggtttcca gggcgtccgt acggggcggg
     7441 ggagcggccc gctcgatgcg gacgacgagg ggcaggacgt actgaggtgc ctcgtcgcgc
     7501 gtcgaaggtt cggtacgaaa ggggctctcg ccgggaacgg agggatcact ggtcacgtcc
     7561 tccagtctgc caggcggaag atccgttctt cggcgcgaga ggatgagccc catgcaacgc
     7621 gatcttcgtc tggacaacgt aggccgccgc tacggcatac gcggcccatg ggtcttacgc
     7681 ggcgtccacc tcaccgtgcc ccccggcacg ctcatccgca tcgacggacc caacggcacc
     7741 ggcaagtcca ccctcctgcg cctcctggcc cgcatagacg ccccctccga aggccgcatc
     7801 accggtcgcc cccgcaccgc gtacgtcccc gaacgcttcc ccacagccct ccccttcacc
     7861 gccctcggct acctgaccca cctcggcacg gtccacggcc tgtcccggcc ggcggccggc
     7921 cgtgccgccc tggagtggct cgaccgcttc ggcgccgccc cgtacgcccg tatgccgatg
     7981 gcccagctgt cgaagggcag cagccagaag gtcgccgtcg tacaggccct cctcgccgaa
     8041 ccggagctgc tcgtcctgga cgaggcgtgg accggcctcg acgccgccgc ccgggaggag
     8101 ctggagcgtg ccgtcgccga acgcacggcg ggcggcaccg ccgtggtctt cgtggaccac
     8161 gacccgcgcc gcctggcggg ggcgcccgac gcgacgtaca ccgtgcgcga cgcgggcctc
     8221 gaccgccgta cggaccaggc gccctcggat cccgacggcc cgcacgtgat cgtcgtcgtc
     8281 cagggcccgc gtggcggaca actccccacc gacgtgcccc gcaccgtcac atcggtcgag
     8341 gaacccacgc ccggcaccca ccgcctcacc gtccccgcgt cccactcgga catcctcctg
     8401 cgcaccctgc tgaccgcccg tccgccatgg cacgtggtga gcgtgaccca ggagagacct
     8461 gcccgacccg aaaccgagag ccgccgatga cgccgatgac accgatgaac gccctcctgc
     8521 gctaccaggc cgccctgctg gcccgctcgc agcgctggct accgccgttc atcctgtacg
     8581 ccgccttcct cggcgtcggt gtgcagagcg ggcagcccgt gctcgactcg ctcggctacg
     8641 ccgccgcggc cctgctgccc gtcgccgcct ggctggtgcg gatctgcgtc accaacgagc
     8701 cgcccgccgc ccgcagttgc gtctccgcgg ccgtcggccc ggggcgggcg cacctcgccg
     8761 cgctgctggt ggcgctggcc gcggcggcgg ccctcggcac ggtggcgacc gtcttcgtga
     8821 ccctgatcag caacccggtc agcgccgacc tccagacccg tgtgccacgg tggcccgcgg
     8881 gcgccgccgg gctgctcgcc gcgctggcct gcgccctgct cggcaccgcc gtcggtgccc
     8941 tcaccacttg gccgctgctg cgctcgaccg gccgcgcggt ccccacgatg ctgctcgcgg
     9001 cgctgctgtc cgtcgtggtg accggctccc cggcccaagc ggcggtcagc ggcctggtca
     9061 ccggctcccg ctcgggcacg gttcccctgc ccctgctgcc cttggccgcg gcggccctgc
     9121 tcacggcggc ggcgacggcc gtggcctgtg cgctcacgtc ccgcagatcg ccctgaacgg
     9181 caggtcaccc cggacggcag gtcaccccga acacgccgtg cgctgcgcct cctgccactc
     9241 gcacaccggg cacagcgtga tccccttgta cgactccggg tattccgtcg gctcccggca
     9301 cagcacgcac tccgcgtagg gcggcccgtc cgccttgggc ggcctggtcg cgttgatcag
     9361 gcagtagtcg gtcgtgggct gttcctcgct catgtcccca gcgtacgagg cccatgtctc
     9421 caccgtacga ggctcatctc tccaccgtac gagcgtcacc gccgcccgcc ggcagccccg
     9481 atcagctcgg acaccttgac gaaccggaag cccttcccgc gcagctcggg cacgacgctc
     9541 cgcaccactc gctcggtcgt cggagcggca ctgagcgtgc agtgcaggac gaccaccgac
     9601 cccgcccgca ccccgtccag cacctctcgg gcgaccgagc gggcgtccgt ggcgaacgcg
     9661 tccccgctca ccacgtccca ctgcacggcg gtgacgccga ccggggtcag cgccttcaga
     9721 gcacgccggt cgtaacagcc gccgggaaac cggaagtacg gcatcgcatc ccgcaccccc
     9781 accttccgga acgaggcgta cgcccgctcc acatccgtcc gcatccgctg ctcgtcgacg
     9841 gtcggcaggc cgtagcagtc gtcggtgaag gcgtggtggc tgtacgagtg gttggcgacc
     9901 tcgaagagcg gatcccgccc gatggcccgg gcctgcaccg gatactcctc ggcccaccgg
     9961 cccgtcatga acacggtcgc cggcaccttc agctcccgca gcgccgcgat cagccccgga
    10021 ttgtcgaacc gttcgccgct cgccgcccgc ggcccctgat cggaggtcat gtccgcatcg
    10081 aaggtgagcg cgacggcctt gtcccgagtg cgggggccgt gctcgaagac cggggtcagg
    10141 ccggcgggcc cgggcgcgag cgtcgggggc tcggccacgg gggaggaggc ggcggtggcg
    10201 ctgggcgccg ggaggggagc gcgggtcgtc tctggcgcac acgaggcgag tgcgctcagc
    10261 gccgcgccca gggcgcagac ggcggtgacg cggcgtacaa gagtgatcac cgcatgaaga
    10321 tatgaggatc aactgctcat atcggtgcgt cgacaggtgt gccgggtccg cgtcactcgc
    10381 ccggcggggc cggtcttctc agtcggtgcg ggtgcaggag cccgtcggtg cggtggccgg
    10441 ggggcggcac tggagggtgt tgtcggtggc gtccgtgctc aggtagcggc cgaggcaggc
    10501 gttcgccgtg gttcggccgc gctcggcgca gaaggtcagg gcggcgcggc cgtcggtgaa
    10561 ggggccgggg gcgtagatca cccagtagcc ggggcgcagg gaggcgtagt cgtcgctgcg
    10621 caggacggtc gcctccggca tggaccggcg gatggtggcg agccgtcggt cccgggcggc
    10681 ggtgcccgag gcgatgggtt cggagtggag ctgggcgatc cagcggcctt ccgacccgcc
    10741 gacgggagtg cgagtggggg tcgtcgggct gggagtcgtc ggagtgggag tcggcgaggg
    10801 ggacggttgc tgtggggccg ggccgggggt ggatctagtg accgccgggg tcgtcgtgcc
    10861 gtcggccttg tcgtcgccgt cggttttgtc ggggcgcagc gcgaagacca gggccgccgc
    10921 cgtggcgatc accagcaccg cgctcgcggc caccacgagt ggtgtacggc gtcgagggcc
    10981 ccgcggctgc tgcggaaccg tcggacgcgg ggtgtccagc tgggtgggga tcggtggcgg
    11041 cgccgggggc gccgtcgggg tcgggtggtg cggggcgccg gcggtcagtg tcggccgagc
    11101 ccagtgcggt gtcgcccctg cctccacctg cgccagcatc ccgtccagtc gggcggcgtc
    11161 cgggcgggcc tccgggtcgc gtaccagcag ggcgtcgagc accggcgtca gcgggcccga
    11221 gcgggtgggg accgggagcg gggcgtcgag tacggcggcc agggtggcga gggtggtggg
    11281 gcggcgcagg gggctgacgc cctccacgca cacgtacagg accacgccca gcgaccacag
    11341 gtcggcggct gggtcgtcgt cgtggccccg gatccgctcc ggggccatgt actcgggcga
    11401 gccgacgagt tcgccggtgg ccgtgagggc ggtcgtgccc tgcagggcgg cgatgccgaa
    11461 gtcggtgagg acggccgccc cgtcggggcg cagaaggatg ttggcgggtt tgacgtcccg
    11521 gtgctggatg cccgccgcgt gcgcggcccg cagcgcggcg agcacctggc ggccgaggcg
    11581 ggccgcctcc accggggtca acgggccgtc gtccaggcgc cgttggagc
//
