LOCUS       NZ_ML138678            10498 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces cyaneus strain CGMCC 4.1671 Scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_ML138678
VERSION     NZ_ML138678.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces cyaneus
  ORGANISM  Streptomyces cyaneus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1603111)
  AUTHORS   Fang,B.-Z., Han,M.-X., Jiao,J.-Y., Xie,Y.-G., Zhang,X.-T., Liu,L.,
            Zhang,Z.-T., Xiao,M. and Li,W.-J.
  TITLE     Streptomyces sp. nov., a novel actinobacterium isolated from a karst
            cave sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603111)
  AUTHORS   Fang,B.-Z., Han,M.-X., Jiao,J.-Y., Xie,Y.-G., Zhang,X.-T., Liu,L.,
            Zhang,Z.-T., Xiao,M. and Li,W.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2018) School of Life Sciences, Sun Yat-sen
            University, No. 135 Xingang West Road, Guangzhou, Guangdong 510275,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-OCT-2018
            Assembly Method                   :: SPAdes v. 3.6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004028635.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 03:28:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,717
            CDSs (total)                      :: 8,635
            Genes (coding)                    :: 8,413
            CDSs (with protein)               :: 8,413
            Genes (RNA)                       :: 82
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 77 of 222
            Pseudo Genes (incomplete)         :: 187 of 222
            Pseudo Genes (internal stop)      :: 12 of 222
            Pseudo Genes (multiple problems)  :: 50 of 222
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:58:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1161826
            Orig. end                         :: 1172324
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ML138678.1.
            Bacteria and source DNA available from CGMCC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10498
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="3"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10498
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10498
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5498](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5498)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(52..68)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.58"
                     /tool="antismash"
     misc_feature    complement(57..71)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.96"
                     /tool="antismash"
     tRNA            364..436
                     /anticodon=(pos:1162223..1162225,aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="EHO50_RS05170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Arg"
     gene            364..436
                     /locus_tag="EHO50_RS05170"
     gene            complement(500..1276)
                     /locus_tag="EHO50_RS05175"
     CDS             complement(500..1276)
                     /NRPS_PKS="Domain: PKS_KR (11-177). E-value: 3.6e-09.
                     Score: 28.7. Matches aSDomain:
                     nrpspksdomains_EHO50_RS05175_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972661.1"
                     /locus_tag="EHO50_RS05175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_164904751.1"
                     /sec_met_domain="adh_short (E-value: 2.7e-31, bitscore:
                     105.3, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTQRALKGKIALVTGAGRGIGRAIAGRLAHDGALVGVHYGHSAD
                     AAQEVVAEIRKNGGRAFPVEAELGVPGDADAVFAAFDAGLRAESGVEGSLDILVNNAGV
                     SGSGPVGQAVPEVFDRMIAVNAKAPFFLVQGALPRMRDGGRIINISSLATRRAFPESVA
                     YAMSKGALDTMTLCLAKELGGRGITVNTVGPGFIETDMNARRRATPEARAALAARSVFD
                     RIGRPSDVADVVAFLACDDSRWITGQYLDVSGGTDL"
     aSDomain        complement(746..1243)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EHO50_RS05175_PKS_KR.1"
                     /evalue="3.60E-09"
                     /label="EHO50_RS05175_PKS_KR.1"
                     /locus_tag="EHO50_RS05175"
                     /protein_end="177"
                     /protein_start="11"
                     /score="28.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="ALVTGAGRGIGRAIAGRLAHDGALVGVHYGHSADAAQEVVAEIRK
                     NGGRAFPVEAELGVPGDADAVFAAFDAGLRAESGVEGSLDILVNNAGVSGSGPVGQAVP
                     EVFDRMIAVNAKAPFFLVQGALPRMRDGGRIINISSLATRRAFPESVAYAMSKGALDTM
                     TLC"
     CDS_motif       complement(1181..1240)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EHO50_RS05175_0001"
                     /evalue="5.20E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="EHO50_RS05175"
                     /protein_end="32"
                     /protein_start="12"
                     /score="9.5"
                     /tool="antismash"
                     /translation="LVTGAGRGIGRAIAGRLAHD"
     gene            complement(1397..2353)
                     /locus_tag="EHO50_RS05180"
     CDS             complement(1397..2353)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (64-172). E-value:
                     2.7e-09. Score: 28.4. Matches aSDomain:
                     nrpspksdomains_EHO50_RS05180_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499749.1"
                     /locus_tag="EHO50_RS05180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_128428570.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5.3e-09, bitscore:
                     31.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPRLLVTGAGGFIGGHVVAALGESRDVTPSRIRLLLRHPGSLPP
                     AGGPVAGAPAADVVRADLADPASLRGVCDGIDVVLHCASHIGDDERLTETVNDHGTRAL
                     VEEATRAGAARVVYVSTAAVYGRGPFTGALPGELPLAPVSPTSRSRAAAERHVLDAGGV
                     VLRPHLVLGAGDRWVVPGLAGLLGLLSAGLKGCTARHSVVDVRALGRAVVAAGLSDRDL
                     TGAHHVNHPEPVDCSELLDTLADRLGLRLPGPPVDIDEARTRLAGRPRALHHLGMLAVD
                     HWFADDRFWGAVGADPGTGFARSLAEHAVWYREFMGR"
     aSDomain        complement(1838..2161)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EHO50_RS05180_NAD_binding_4.1"
                     /evalue="2.70E-09"
                     /label="EHO50_RS05180_NAD_binding_4.1"
                     /locus_tag="EHO50_RS05180"
                     /protein_end="172"
                     /protein_start="64"
                     /score="28.4"
                     /tool="antismash"
                     /translation="DPASLRGVCDGIDVVLHCASHIGDDERLTETVNDHGTRALVEEAT
                     RAGAARVVYVSTAAVYGRGPFTGALPGELPLAPVSPTSRSRAAAERHVLDAGGVVLRPH
                     LVLG"
     misc_feature    complement(2339..2341)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2459..3418
                     /locus_tag="EHO50_RS05185"
     CDS             2459..3418
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="EHO50_RS05185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_128428571.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.1e-54, bitscore:
                     180.8, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQSVSTEVADFSPLADDVEVVPFTTHLPDEDALAA
                     ALADFDIVVTLRERVPFPASLIARLPRLKLLIASGMRNSVIDYAAAEAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVEESNALRSGGPWQHTVGADLHGRRLGLLGLGKIGSRVAQ
                     VGLAFGMHVTAWSQNLTKEYADEVGVQLAPSKEELLAESDFVSVHLALSDRTRGLLGAS
                     ELALLKPTAYLINTSRAAIVDQDALLTALHEGRIAGAGVDVFDIEPLPADHPMRTAPRL
                     LATPHLGYVSQANYATYYGQAVENIQAYLAGSPVRRLP"
     gene            complement(3462..4058)
                     /locus_tag="EHO50_RS05190"
     CDS             complement(3462..4058)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136071.1"
                     /locus_tag="EHO50_RS05190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_128428572.1"
                     /transl_table=11
                     /translation="MATDPTEQLVRLFAEESRVRAFAAVALGAGSSEQVARAAGLSPKE
                     TAVALLRLREQGVVTAGDDGELGVAYGLFKEYAREAARRENDAAAAGSPASGDDRTDLV
                     LRTFVRDGRLVRLPARWTRKKLVLRHIAEQTFEPGVEYPERTVDDKLRVWCEDSDDIDH
                     VALRRYLVDLHHLSRSEGIYRRPVEAAHDGDADIA"
     misc_feature    complement(4027..4041)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.09"
                     /tool="antismash"
     misc_feature    4028..4042
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.94"
                     /tool="antismash"
     gene            complement(4157..5017)
                     /locus_tag="EHO50_RS05195"
     CDS             complement(4157..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="EHO50_RS05195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_128428573.1"
                     /transl_table=11
                     /translation="MVYVRKNVSKLTRTERRRFVEALLELKRSGEYDEFVRLHIEHFVG
                     DGDRGLRAAHMTPSFLPWHRRFVLDMESALQRVDPSVTVPYWDWTRDRSTTSVPWTDDL
                     LGGTGRPVDRQVMTGPFAYRTGNWTITESITNVEFLTRNLGRPRDPIDLPTRDDLNWAL
                     KDPVYDVAPWDSTVTKGFRNKMEGWGSGRVVWRNHNRVHRWVGGVMLGGASVNDPVFWL
                     VHAFMDMQWQRWQRRHRKHRYLPAKPPGRGSAQYGRIVARHERLPPWDVTPDELEDMSA
                     IYRYA"
     gene            complement(5001..5498)
                     /locus_tag="EHO50_RS05200"
     CDS             complement(5001..5498)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065674.1"
                     /locus_tag="EHO50_RS05200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_128428574.1"
                     /sec_met_domain="melC (E-value: 4.4e-45, bitscore: 149.7,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVSADGVSVGAVQGAGAPVSGETGRWTRREAARLLFASVAVAFT
                     PVLAASRPSQSADGQHETGTPFDETYRGRRIQGAPLPFEGLSALGVEWRVTVDGRPLHL
                     MRRADGTWLSMIDHYSWYRTPLEATRAAVDELGPRRRLLDLAPGPFHGGHLHMEAEHGV
                     RA"
     gene            complement(5600..6208)
                     /locus_tag="EHO50_RS05205"
     CDS             complement(5600..6208)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001130.1"
                     /locus_tag="EHO50_RS05205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_128428575.1"
                     /transl_table=11
                     /translation="MTPTPAPGARRTVGGGRAFALLLVLTGAAGLLASWVITLDKFKLL
                     EDPDFTPGCSLNPVLSCGSVMESDQAEAFGFPNPMLGLVAYGIVICVGVSLLAGAAFPR
                     WYWLAFGAGCLFGIGFVSWLQFESLYRINALCLWCCLAWLATIPLFWYVTSFLVRNGFL
                     PAPGPVKAFLAEFTWVLPLLHIGIVGMLILTRWWDFWTS"
     gene            complement(6205..6942)
                     /locus_tag="EHO50_RS05210"
     CDS             complement(6205..6942)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435079.1"
                     /locus_tag="EHO50_RS05210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-glucanase"
                     /protein_id="WP_128428576.1"
                     /transl_table=11
                     /translation="MTTDSVKFTPAATADRSLIWSEEFSTPIAWGAKWVGDRTSAYRYC
                     DHNPDDNKLDRLDPGCVTVSGGTATFTATPSGHTLENGRQAWRTGLLTTEYSDEGFRVR
                     TGDYVEARLQLPSRLGAWPALWTWKDGENEVDSFEYHPDNPHLLELSNHVNCGSTYYTD
                     EDAIAPDSWVTIGTCYGACSVEWYVNGACVFSDGTGVGADWSAYLILNLSVCAGEHHPA
                     PCGPAPITFGVDYLRVHREAARW"
     gene            7176..7568
                     /locus_tag="EHO50_RS05215"
     CDS             7176..7568
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499736.1"
                     /locus_tag="EHO50_RS05215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_246101021.1"
                     /transl_table=11
                     /translation="MKKAVFTTAALAIGAGLVGGSAAAASAAGGSGISTSPIGGLMSPT
                     KVSPAILPAADAVTRLAREGGVAKGSAFDKNKSGGMSGVYLDGMDPNAAKKPTIPVKNP
                     VGTGSVQVHPLHAPTASGLGTLLPKK"
     misc_feature    7741..7755
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.09"
                     /tool="antismash"
     gene            8042..8377
                     /locus_tag="EHO50_RS05220"
     CDS             8042..8377
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001127.1"
                     /locus_tag="EHO50_RS05220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_128428577.1"
                     /transl_table=11
                     /translation="MSKKFAVITALALSTVVGTAGLASAGEYDGNGKTTTVKGGSGFST
                     APVVVNAPQQGILVVNGTVVDGRCLAPWSNGAVLGGVVPPNSHYAACNTAEVNQAQNAP
                     YAGGLLF"
     gene            8440..8760
                     /locus_tag="EHO50_RS05225"
     CDS             8440..8760
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001125.1"
                     /locus_tag="EHO50_RS05225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_128428578.1"
                     /transl_table=11
                     /translation="MRKKVTAISALAAGIALATGGTAAADADTTTVKGGSGFSTSPVVV
                     NAPQQGAVIVNGTAVDLRCAVPWSNGAVLGGVVAPNSHYASCNTAKIDQSQNALYKGGL
                     LF"
     misc_feature    8803..8821
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     gene            8821..9141
                     /locus_tag="EHO50_RS05230"
     CDS             8821..9141
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001125.1"
                     /locus_tag="EHO50_RS05230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_062714413.1"
                     /transl_table=11
                     /translation="MRKKVTAISAIAAGIVLATGGTAAADGDTTTVKGGSGFSTSPVVV
                     NEPQQGIAVVNGALLDARCLAPWSNGAVLGGVAAPNSHYAACNTAKVDQAQNAPYVGGL
                     LF"
     gene            complement(9501..9803)
                     /locus_tag="EHO50_RS05235"
     CDS             complement(9501..9803)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001124.1"
                     /locus_tag="EHO50_RS05235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_128428989.1"
                     /transl_table=11
                     /translation="MKNLKAAAVVAGSLAVAGVAGPAAAVDMPAQGLVDNGKTVARSLP
                     DAAKLPTGYVGNNVKRTTDGVKQGVKKSSVVKTPVFGGTLPNPVDGQLLGGLPIR"
     misc_feature    10043..10054
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.89"
                     /tool="antismash"
ORIGIN
        1 agtgcgtcga gcagctgagg ccgtacgtcc aaggtcacca catgcgaaga atgcccaatt
       61 ttaggggaat tctgaagcat atgagcatgt ctgcgcgccg acgggacaca agggatggaa
      121 ggggcgactg ggacacaggc acagtccagg cataacgagt gcacaacacc cggggcgtac
      181 gaggccaaaa gcggtgcacg attcgcacct gcctgcgatt atcaggaccg cttcaccgaa
      241 cctccacatc gagccacgga ggcgaagcaa ctgggacatg gacgcgggag ccctatcctt
      301 gtccccggca tcgtcacgga tcagcccgca ccccgggcac cacgaccgag gccaccacaa
      361 ccggcccccg tagctcagtg gatagagcag gcgccttcta agcgcttggc cgcaggttcg
      421 agtcctgccg ggggcgctca tgctccgccc tccttcggga gggcgttttt gctgccgctc
      481 cctcacgtcc ggggcggcgt cacaggtcgg tgcccccgct gacgtcgagg tactgaccgg
      541 tgatccagcg ggagtcgtcg caggcgagga acgccaccac gtcggccacg tcggagggcc
      601 tgccgatgcg gtcgaagacg gaccgcgcgg ccagggcggc ccgtgcctcg ggcgtcgctc
      661 tgcgccgggc gttcatgtcc gtctcgatga accccggccc gaccgtgttg acggtgatgc
      721 cgcggccgcc gagctccttc gccagacaca gggtcatggt gtccagcgca cccttggaca
      781 tggcgtacgc gacggactcg gggaaggccc gtcgtgtggc gagcgaggag atgttgatga
      841 tgcgcccacc gtcacgcatc cgcggcagcg cgccctgcac gaggaagaac ggtgccttgg
      901 cgttgacggc gatcatgcgg tcgaagacct cgggaaccgc ctgccccacc ggccccgaac
      961 cgctcacccc cgcgttgttc accaggatgt cgagcgagcc ctcgacgccg ctctccgcgc
     1021 gcagcccggc gtcgaaggcc gcgaacacgg cgtcggcatc gcccggcacg ccgagttccg
     1081 cctcgaccgg gaaggctcgt ccgccgttct tgcgtatctc cgccacgacc tcctgggcgg
     1141 cgtccgcgct gtgcccgtaa tggacgccga cgagcgcccc gtcgtgcgcg agccgcccgg
     1201 cgatggctct gccgattccc cggccggccc cggtgaccag tgcgatcttg cccttcagtg
     1261 cgcgttgggt atccacgaag acctcacgaa gaccttgtgt atcgaaggaa gcaggaacga
     1321 gggagagagt aggaacgaag gagagagcag gaacggagga gggagcagag gagagaaagc
     1381 gtgccggacc gacgcctcac cggcccatga actcccggta ccacacggcg tgttcggcaa
     1441 ggctccgcgc gaaccccgtt ccgggatcgg ccccgacggc gccccagaac ctgtcgtccg
     1501 cgaaccagtg gtccacggcg agcatgccga ggtggtgcag cgcacgcggc cggccggcga
     1561 gccgggtccg ggcctcgtcg atgtccacgg gcgggcccgg caggcgcagg ccgagccggt
     1621 cggccagcgt gtccagcagt tccgaacagt ccaccggctc cgggtggttg acgtgatgcg
     1681 cgcccgtcag gtcccggtcc gacagcccgg cggcgaccac cgcacgcccc aacgcccgta
     1741 cgtcgacgac ggagtgccgg gcggtgcagc ccttcaggcc ggccgacagc agccccagca
     1801 ggccggccag ccccggcacc acccatcggt cgccggcccc gagcacgagg tgcgggcgca
     1861 gcacgacccc gccggcgtcc agtacgtgcc gctcggcggc ggcgcgggac cggctggtcg
     1921 gcgagaccgg cgcgagcggc aactcgccgg ggagcgctcc ggtgaagggc ccccggccgt
     1981 atacggcggc cgtactcacg tagacgaccc gggccgcacc tgcgcgcgtc gcctcctcga
     2041 cgagggcgcg ggtgccgtgg tcgttgacgg tctcggtgag gcgttcgtcg tcgccgatgt
     2101 gggaggcgca gtgcagtacg acgtcgatgc cgtcgcagac accgcgcagg gacgccgggt
     2161 cggcgaggtc cgcgcgtacg acatccgccg ccggggcgcc ggcgacgggg ccgccggccg
     2221 gcggcaggct cccggggtgg cgcagcagca gccggatccg ggacggcgtc acgtcccgcg
     2281 actcaccgag agccgcgacg acatgacctc cgatgaagcc gccggcaccg gtgacgagta
     2341 agcgtggtga caaggtgagt tcctccctgc cggcccacgg accggctcgg cggccaggtc
     2401 cttcacggta ctgcgcgttc cgtacagtcg ttccacctgg ccatggaaag gaaccaccgt
     2461 gcggctgcgc tgtgctgtgc tcgacgactt ccagagcgtg tcgaccgagg tcgccgactt
     2521 ctcgcccctc gcggacgacg tggaggtcgt ccccttcacc acccacctcc ccgacgagga
     2581 cgccctcgcc gcggccctcg ccgacttcga catcgtcgtc acgctgcggg aacgcgtccc
     2641 cttccccgcc tccctgatcg cccgtctgcc ccggctgaag ctgctgatcg cctccggcat
     2701 gcgcaactcg gtcatcgact acgccgccgc cgaggcgcac ggcgtcaccg tctgcggtac
     2761 ggcgagctcc tcgaccccgc ccgtcgaact cacctgggcc ctactgctcg gcctcgcccg
     2821 cggcatcgtc gaggagagca acgccctgcg ctccggtggc ccctggcagc acacggtcgg
     2881 cgccgacctg cacggccgcc gtctcggact gctcggcctg ggcaagatcg gcagccgggt
     2941 ggcccaggtc ggcctcgcct tcggcatgca cgtcaccgcc tggagccaga acctcaccaa
     3001 ggagtacgcc gacgaggtgg gcgtccaact ggctccctcc aaggaggagt tgctcgccga
     3061 gagcgacttc gtctccgtcc acctggccct gagcgaccgc acccgaggcc tgctcggtgc
     3121 gtcggaactc gccctcctca agccgacggc gtacctgatc aacacctcac gcgcggcgat
     3181 cgtcgaccag gacgccctgc tcaccgccct gcacgagggc cgcatcgccg gtgccggcgt
     3241 cgacgtcttc gacatcgagc ccctccccgc cgaccacccg atgcgcacgg ccccccgcct
     3301 gctcgccaca ccccacctgg gctatgtctc gcaggccaac tacgccacgt actacggcca
     3361 ggcggtcgag aacatccagg cgtacctggc gggttcaccg gtacgacggc tgccttgagg
     3421 ccggcgctgc cgcggcgctc cggcgggggg tcagtcagcg gtcaggcgat gtcggcgtcc
     3481 ccgtcgtgcg cggcctcgac cggccgccgg tagatcccct cgctccggct cagatggtgc
     3541 aggtccacca gatagcgccg gagcgccaca tggtcgatgt cgtcgctgtc ctcgcaccag
     3601 acgcgcagct tgtcgtcgac ggtccgctcc gggtactcga cacccggctc gaaggtctgc
     3661 tcggcgatgt ggcgcagcac cagcttcttg cgggtccacc gcgcgggcag ccggaccagc
     3721 cgcccgtccc ggacgaaggt gcgcaggacc aggtcggtcc ggtcgtcccc ggaggcaggg
     3781 ctcccggcgg ccgcggcgtc gttctcccgc cgcgccgcct cccgcgcgta ctccttgaac
     3841 aggccgtacg ccacgcccag ttccccgtcg tccccagccg tcaccacgcc ctgctcccgc
     3901 agtcggagca gcgccacggc cgtctccttc ggggacagcc ccgccgcccg ggcgacctgc
     3961 tcactgctcc ccgcgcccag cgccacggcg gcgaaggccc gcacccggct ctcctcggcg
     4021 aacagccgga ccagttgttc cgtcggatcg gtagccatgc ccggaggcta gtggacatcg
     4081 gccgcggagc taaccgattt ttccgcgaac cgaaacgagg cgaggcgagg cgacgcagga
     4141 catggcgagg cgggcctcag gcgtaccggt agatcgcgga catgtcctcc agctcgtccg
     4201 gagtcacgtc ccacggtggc agtcgctcgt gccgggcgac gatccggccg tactgcgcgc
     4261 tgccccggcc gggcggcttc gccggcagat agcggtgttt gcggtggcgc cgctgccagc
     4321 gttgccactg catgtccatg aaggcgtgga ccagccagaa gacggggtcg ttgacggacg
     4381 cgccgccgag catgacgccc ccgacccagc gatgcacccg gttgtggttg cgccagacga
     4441 ccctgccgct gccccacccc tccatcttgt tgcggaagcc cttggtgacc gtcgagtccc
     4501 acggggcgac gtcgtagacg gggtccttca gcgcccagtt cagatcgtcc cgggtgggca
     4561 ggtcgatcgg atcgcggggg cggcccaggt tccgggtgag gaactcgacg ttggtgatgc
     4621 tctccgtgat ggtccagttg cccgtccggt aggcgaacgg cccggtcatc acctgccggt
     4681 ccacgggccg cccggtgccg ccgagcagat cgtcggtcca cggcaccgag gtggtcgaac
     4741 ggtcccgggt ccaatcccag tacggcaccg tcaccgaggg gtcgacacgc tgcagggcgc
     4801 tctccatgtc cagcacgaac cggcggtgcc agggcaggaa ggagggcgtc atatgggcgg
     4861 cgcgcagtcc gcggtcgccg tcgccgacga agtgctcgat gtgcagccgt acgaactcgt
     4921 cgtactcgcc actgcgtttg agctcgagca gcgcctcgac gaaccgccgc cgctcggtgc
     4981 gcgtcagctt gctgacgttc ttacgcacgt acaccatgct ccgcctccat gtgcaggtgc
     5041 ccgccgtgga acggcccggg agccaggtcg agcagccgtc ggcggggtcc gagttcgtcg
     5101 acagccgcgc gggtcgcctc cagcggcgtc cggtaccagc tgtagtggtc gatcatgctc
     5161 agccaggtgc cgtcggcgcg gcgcatcagg tgcagcgggc ggccgtccac ggtgacccgc
     5221 cattcgacgc cgagggcgct gaggccctcg aagggaagcg gggcgccctg gatacggcgg
     5281 ccgcggtagg tctcgtcgaa gggggtgccg gtctcgtgct gcccatccgc ggactgcgag
     5341 ggccgggagg cggcgagcac cggcgtgaac gccacggcga cggaggcgaa cagcagccgc
     5401 gccgcctccc gccgtgtcca ccgccccgtc tcgccgctca ccggggcgcc cgccccctgt
     5461 acagcaccca ccgacactcc gtctgcgctg acggccatcg ctcactcctc gtacggttcg
     5521 gctgttcgta cgagtaaccg tccaggacgc cccacggtca ccgccggaac gcccatacgg
     5581 cggtgtcacc cggtcggcct caactcgtcc agaagtccca ccaacgggtc aggatcagca
     5641 tcccgacgat cccgatgtgc agcagcggaa gtacccaggt gaactccgcg aggaaggcct
     5701 tcaccggccc cggcgcgggc aggaagccgt tgcgcacaag aaaagaagtg acgtaccaga
     5761 acagcgggat cgtcgcgagc caggccaggc agcaccacag gcacagcgcg ttgatccggt
     5821 acagggactc gaactgcagc caggagacga acccgatccc gaagaggcat cctgccccga
     5881 aggccagcca gtaccagcgc gggaaggcgg ccccggccag cagactgacg ccgacgcaga
     5941 tcacgatgcc gtaggccacc aggcccagca tcgggttggg gaacccgaag gcctcggcct
     6001 ggtcgctctc catcacgctg ccgcaggaca gcacggggtt caggctgcag cccggggtga
     6061 agtccgggtc ctcgagcagc ttgaacttgt cgagggtgat gacccaggag gccagcagcc
     6121 cggccgcccc ggtgagcacg agcagcaggg cgaacgcccg gccgccgccc acggtccgcc
     6181 gcgcgcccgg ggccggggtc ggggtcacca ccgcgctgcc tcgcggtgga cgcggaggta
     6241 gtcgacgccg aaggtgatcg gagccgggcc gcagggcgcc gggtggtgct cgccggcgca
     6301 caccgacagg ttcaggatca gatacgccga ccagtcggcg ccgacgccgg tgccgtcgga
     6361 gaagacgcac gcgccattga cgtaccactc gacggaacac gcgccgtagc aggtgccgat
     6421 ggtcacccag ctgtccgggg cgatggcgtc ctcgtcggtg tagtacgtcg aaccgcagtt
     6481 gacgtggttg gacagctcca gcagatgcgg gttgtccggg tggtactcga aggagtcgac
     6541 ctcgttctcg ccgtctttcc aggtccacag cgccggccag gccccgagcc gggagggcag
     6601 ttggaggcgg gcctcgacgt agtcgccggt cctcacccgg aagccctcgt cggagtactc
     6661 ggtggtgagc aggccggtgc gccaggcctg gcggccgttc tccagggtgt ggccggaggg
     6721 tgtggcggtg aaggtggccg tgccgcccga gacggtcacg cagccggggt cgagtcggtc
     6781 gagcttgttg tcgtccgggt tgtgatcgca gtagcggtag gcgctcgttc tgtcgccgac
     6841 ccatttggcg ccccaggcga tcggtgtgct gaactcctcc gaccagatga gcgacctgtc
     6901 ggcggtcgcg gccggcgtga acttgacgct gtcggtggtc atggcggcga gggtaattcc
     6961 tctttcgggt cgcgcatgcg gtcgaccggg ctggacgaac catacgatca atcgattcgc
     7021 cgaccagatg tcaatccctc cgaatgcctc tgtgcattcc attcttcacc cctgcgaatt
     7081 tcacgcgtat ggagcaacgt gtctcttttg cagcggcctt ggactgctat gttctccaac
     7141 cgtcggatca gaggtacttt caagcgaggt gaatcatgaa gaaggcagtt ttcaccactg
     7201 ctgcgttggc catcggtgcg ggtcttgtgg gtggctcggc cgctgcggcg tcggccgccg
     7261 ggggatcggg aatctccacc agtccgatcg gggggctcat gtccccgacc aaggtgagcc
     7321 cggcgatcct gcccgcggcc gatgccgtga cacggctggc ccgcgaaggc ggtgttgcaa
     7381 agggcagcgc gttcgacaag aacaagtccg ggggaatgtc cggcgtttac ctggacggaa
     7441 tggacccgaa tgccgccaag aagcccacga ttccggtgaa gaacccggtc ggaaccggat
     7501 cggtccaggt gcacccgctg catgcgccga ccgcttcggg gctcggcacc ttgctcccca
     7561 agaagtgaag accttggtcc cgtatgttcg cgggacagag ctgttcccgg aattctgaga
     7621 ctctgatccg gttctcggga gccggagacc gacagcaggc ccagacgctg gatccggacc
     7681 cgcagctccc cctccgcaca cggcgggccc gaagcccact gcacccgcga accatctgtc
     7741 cggcccaccg gccctccccc tgccggtggg ccggacgcat gtcggccgcc gttccggccg
     7801 gctccattcg gcccaatgca cccgcatttt cccggaacac caggtgattc gcgccggttc
     7861 ccgcatggcc gggaccttct cccggaatga acgtaaagcg ggttgtgacg gcgggtcagg
     7921 ctccgttgcg ccatccgaag gaatctggcc acgtttgatt gttcccgccg tgacttaggc
     7981 gcgcatatac gggtttcctt ttcgtaagcg tgcgcaaccg tgaattcagg aaggaaccga
     8041 gttgtccaag aagttcgcgg tcatcacggc cctcgccctc agcaccgtcg tcggaactgc
     8101 aggcctggcc tctgctggtg aatacgacgg caacggcaag accaccaccg tcaagggcgg
     8161 tagcggattc tcgacggccc ccgtcgtcgt gaacgctccc cagcagggca ttctcgtcgt
     8221 caacggcacc gtcgtcgacg gtcgctgcct cgccccgtgg tccaacggag ccgtccttgg
     8281 cggcgtcgtc ccaccgaact cgcactacgc cgcctgcaac acggccgagg tcaaccaggc
     8341 gcagaacgcc ccgtacgcgg gcggcctgct gttctgacct ctgctccgaa ctattcgcag
     8401 cacctgaatc accccaacgc agcccgaaag gcgcaactca tgcgtaagaa ggttacggcc
     8461 atctccgcgc tcgcggcagg catcgccctc gcgaccggcg gcaccgccgc ggccgacgct
     8521 gacaccacca ccgtcaaggg cggtagcggc ttctcgacgt ccccggtcgt cgtgaacgcg
     8581 ccccagcagg gcgccgtcat cgtgaacggc acggccgtcg acctgcgctg tgccgtgccg
     8641 tggtccaacg gagccgttct cggcggcgtc gtcgcgccga actcgcacta cgcctcctgc
     8701 aacacggcca agatcgacca gtcgcagaac gccctgtaca agggcggcct gctgttctga
     8761 ctcctgctcc gagcattcgg agcaggtaaa tcccgaacgc agcccggaaa ggctcaactt
     8821 atgcgtaaga aggttacggc catctccgcc atcgccgcgg gcatcgtcct cgcgaccggc
     8881 ggcaccgccg cggccgacgg tgacaccacc acggtcaagg gcggtagcgg cttctcgacg
     8941 tctccggtcg tcgtgaacga accccagcag gggatcgccg tcgtcaacgg cgccctcctc
     9001 gacgctcgct gcctcgcccc gtggtccaac ggagccgttc tcggcggcgt cgctgctccg
     9061 aactcgcact acgcggcctg caacacggcc aaggtcgacc aggcgcagaa cgccccgtac
     9121 gtgggcggcc tgctgttctg agcagtgctc cagccgatgg cggtccgccg gaaacccggc
     9181 aggccgccat cggcttttgt gcacggtgat ttcggagatc tcggcaccaa actcgtgggc
     9241 cctgtctgca gcggtacgca gacagggccc aaatgaggga gcgccaacaa ggaggaatga
     9301 gcgcccattc gattggacca acggtgctgg gccgtctgtg gtcaccctgg ttccttcgtt
     9361 tggcccaatg cggcgaagaa taaaagaaca tgacgataaa agaacatgac gaagggccgg
     9421 ctccgagaga tacggagccg gcccggaacg tcgagccgat gggcgacgaa gtcgaggaaa
     9481 cgaatcgcct acctgatcgc ctacctgatc gggagtccgc cgagcagctg gccgtcgacg
     9541 gggttcggca gcgtgccgcc gaagacgggg gtcttcacca cggacgactt cttcacgccc
     9601 tgcttcacac cgtccgtcgt gcgcttcacg ttgttgccca cgtagcccgt cggcagcttg
     9661 gcggcgtcgg ggaggctacg ggccaccgtc ttgccgttgt ctacgaggcc ctgcgccggc
     9721 atgtccaccg cggcggcggg gccggcgact ccggcgacag cgagggagcc ggcgacgacg
     9781 gcagcagcct tcaggttctt catcatgttc ctttcgtggg gactcgactt cagtaactcg
     9841 cgcccgccgg acgggatttc acgcctcggg cgtcgcgagt gactcggtct cggttctcct
     9901 gggctaacga caggggatcg ccgtggaaac tctgctgccg acaagttcca ggaactcata
     9961 cgaaaagacc cgctgcgccg cgggcacggg ccccgaacgg cccatggcac caaacgggtg
    10021 gtcccgtacg agaaaccact cagaaaacgc gatcattcca ccatcgttca ccggatggga
    10081 gataaatatg gataagtggc tggtgctgct cgtcccatga ctccaggaag ccgaacgcga
    10141 gccacacatc gtgttgctgg ttcggactct tttcgtcgga agggcggcct cggtcatgcg
    10201 cagttccgtg ccgcacattc tgcatgtcgc tcagcccgtc gagggcggag tcgcgcgggt
    10261 cgtgacggac ctggtggcgg cacagctcgc cgccgggctg cgggtgacgg tggcctgtcc
    10321 ggagggcggc acgctggcgg acggcctgcg cgggccgggc cgcacagtgc tccgctggga
    10381 cgcgacccgc tcaccggggc gtcgactccc gggcgaggta cggcagttgg cgcggctggt
    10441 gcgcgaggtg cggccggacc tggtgcacgc gcacagcgcg aaggcggggc tcgcggcc
//
