GCF_004010955.1 - 11 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP025227.1 (Actinomyces wuliandei)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	butyrolactone	60,846	71,775
Region 2	transAT-PKS,NRPS	464,320	529,330
Region 3	terpene-precursor	565,173	586,282
Region 4	azole-containing-RiPP	713,152	734,963
Region 5	lanthipeptide-class-ii	986,439	1,009,108
Region 6	linaridin	1,150,801	1,177,227
Region 7	terpene-precursor	1,261,522	1,282,676
Region 8	lanthipeptide-class-ii	1,794,934	1,818,263
Region 9	terpene-precursor	1,834,353	1,855,411
Region 10	NRP-metallophore,NRPS	2,570,568	2,629,912	Low	benarthin/dibenarthin	NRPS:Type I
Region 11	phosphoglycolipid	2,689,666	2,711,566

NZ_CP025227.1 - Region 1 - butyrolactone

Download region GenBank file

Download region SVG

Location: 60,846 - 71,775 nt. (total: 10,930 nt) Show pHMM detection rules used

butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

CWS50_RS00290: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CWS50_RS00320: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase

CWS50_RS00275: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: synthase

CWS50_RS00310: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002276.2	0.57	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000234.5	0.46	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002510.2	0.46	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0000231.5	0.45	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002711.2	0.44	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001723.5	0.43	PKS	oviedomycin	Streptomyces antibioticus
BGC0000243.4	0.43	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0002547.2	0.42	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces youssoufiensis
BGC0000272.3	0.42	PKS	spore pigment	Streptomyces collinus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002276.2	0.57	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000234.5	0.46	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002510.2	0.46	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0000231.5	0.45	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002711.2	0.44	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001723.5	0.43	PKS	oviedomycin	Streptomyces antibioticus
BGC0000243.4	0.43	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0002547.2	0.42	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces youssoufiensis
BGC0000272.3	0.42	PKS	spore pigment	Streptomyces collinus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.33 out of 25.75

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.85 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.63 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.40 out of 29.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.48 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_CP025227.1 - Region 2 - NRPS,transAT-PKS

Download region GenBank file

Download region SVG

Location: 464,320 - 529,330 nt. (total: 65,011 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

CWS50_RS01925: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

CWS50_RS13920: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

Transferases: acylase aminotransferase kinase

CWS50_RS01910: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

CWS50_RS02000: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

CWS50_RS02065: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: back translocase

CWS50_RS02050: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

Ligases: ligase synthase

CWS50_RS01985: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CWS50_RS01990: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CWS50_RS02015: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.51	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002826.2	0.39	other (other)	closoxazole A, closoxazole B	Clostridium cavendishii DSM 21758
BGC0002171.2	0.33	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.33	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001050.5	0.32	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0000013.3	0.31	PKS	alternariol	Aspergillus nidulans FGSC A4
BGC0000851.5	0.29	other (other)	albonoursin	Streptomyces noursei ATCC 11455
BGC0001295.5	0.29	other (other)	cremeomycin	Streptomyces cremeus
BGC0001109.4	0.28	NRPS, PKS	pederin	unidentified unclassified sequences.
BGC0000885.3	0.28	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002826.2	0.52	other (other)	closoxazole A, closoxazole B	Clostridium cavendishii DSM 21758
BGC0002171.2	0.48	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.48	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001050.5	0.46	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0000013.3	0.46	PKS	alternariol	Aspergillus nidulans FGSC A4
BGC0001295.5	0.44	other (other)	cremeomycin	Streptomyces cremeus
BGC0000851.5	0.44	other (other)	albonoursin	Streptomyces noursei ATCC 11455
BGC0001109.4	0.44	NRPS, PKS	pederin	unidentified unclassified sequences.
BGC0000885.3	0.43	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.01 out of 29.38

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.13 out of 30.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.37 out of 27.78

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.31 out of 30.22

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.67 out of 36.53

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 464319 - 529330:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (X - mal - Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CWS50_RS01985: X - Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (17..402): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RWMPIMPSLWEGTLTVGGNLEDYGATEANTIAAG
Nearest Stachelhaus code(s):: 3,4-dohBza MPWAIGDVLK (53% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (2097..2496): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: LDLNFDLTIFDGNSFLSGDVALGGATEAVVWSNL
Nearest Stachelhaus code(s):: Cys DLFNFSLVWK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

CWS50_RS01990: X

AMP-binding (78..461): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GDNPHNASLAFHSAISSGD-----------HSVR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

CWS50_RS02000: mal

PKS_AT (4..290): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 90.3
Methylmalonyl-CoA: 44.4
Methoxymalonyl-CoA: 39.9
inactive: 35.3
Propionyl-CoA: 26.1
Isobutyryl-CoA: 15.3
fatty_acid: 14.1
trans-1,2-CPDA: 13.2
2-Methylbutyryl-CoA: 12.1
Ethylmalonyl-CoA: 11.2
CHC-CoA: 10.5
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_CP025227.1 - Region 3 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 565,173 - 586,282 nt. (total: 21,110 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

CWS50_RS02280: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis
BGC0001227.3	0.37	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000678.5	0.36	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000643.3	0.35	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.35	terpene	carotenoid	Brevundimonas sp. SD212
BGC0000648.3	0.34	terpene	carotenoid	Myxococcus xanthus
BGC0000905.5	0.34	other (other)	heme D1	Heliobacillus mobilis
BGC0000906.3	0.34	other (other)	heme D1	Heliophilum fasciatum
BGC0000665.5	0.33	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001594.3	0.33	other (other)	fischerindole L	Fischerella muscicola UTEX 1829

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis
BGC0001227.3	0.37	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000678.5	0.36	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000643.3	0.35	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.35	terpene	carotenoid	Brevundimonas sp. SD212
BGC0000648.3	0.34	terpene	carotenoid	Myxococcus xanthus
BGC0000905.5	0.34	other (other)	heme D1	Heliobacillus mobilis
BGC0000906.3	0.34	other (other)	heme D1	Heliophilum fasciatum
BGC0000665.5	0.33	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001594.3	0.33	other (other)	fischerindole L	Fischerella muscicola UTEX 1829

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

CWS50_RS02280: PT_FPPS_like

Domain 1 (38 - 317): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.42 out of 21.55

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_CP025227.1 - Region 4 - azole-containing-RiPP

Download region GenBank file

Download region SVG

Location: 713,152 - 734,963 nt. (total: 21,812 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase

CWS50_RS02870: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

CWS50_RS02875: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

CWS50_RS02935: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: kinase

CWS50_RS02855: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.53	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0000569.5	0.51	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001173.5	0.50	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000568.5	0.47	ribosomal	microcin B17	Escherichia coli
BGC0002013.4	0.45	ribosomal	curacozole	Streptomyces curacoi
BGC0000566.5	0.42	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0001170.5	0.42	ribosomal	clostridiolysin S	Clostridium sporogenes ATCC 15579
BGC0000564.5	0.42	ribosomal	clostridiolysin S	Clostridium botulinum A str. ATCC 3502
BGC0000504.5	0.40	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000491.5	0.40	ribosomal	gassericin A	Lactobacillus gasseri

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.53	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0000569.5	0.51	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001173.5	0.50	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000568.5	0.47	ribosomal	microcin B17	Escherichia coli
BGC0002013.4	0.45	ribosomal	curacozole	Streptomyces curacoi
BGC0000566.5	0.42	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0001170.5	0.42	ribosomal	clostridiolysin S	Clostridium sporogenes ATCC 15579
BGC0000564.5	0.42	ribosomal	clostridiolysin S	Clostridium botulinum A str. ATCC 3502
BGC0000504.5	0.40	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000491.5	0.40	ribosomal	gassericin A	Lactobacillus gasseri

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.59 out of 27.78

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.67 out of 23.79

Confidence: medium

Additional weak hits (8):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.89 out of 33.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.54 out of 28.14

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.81 out of 36.54

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.10 out of 26.85

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.95 out of 39.62

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.08 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.23 out of 28.14

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

TFBS Finder

Azole RiPPs

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
IolR	Regulator of the inositol catabolism

Core peptide predictions

No core peptides found.

NZ_CP025227.1 - Region 5 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 986,439 - 1,009,108 nt. (total: 22,670 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

CWS50_RS04105: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002698.2	0.58	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000551.5	0.55	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0001555.4	0.52	ribosomal	colicin V	Escherichia coli chi7122
BGC0000590.5	0.51	ribosomal	microcin N	Escherichia coli
BGC0000588.5	0.49	ribosomal	microcin L	Escherichia coli
BGC0002699.2	0.48	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0000519.4	0.45	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000501.3	0.45	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000517.4	0.45	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0001229.4	0.45	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002698.2	0.58	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000551.5	0.55	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0001555.4	0.52	ribosomal	colicin V	Escherichia coli chi7122
BGC0000590.5	0.51	ribosomal	microcin N	Escherichia coli
BGC0000588.5	0.49	ribosomal	microcin L	Escherichia coli
BGC0002699.2	0.48	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0000519.4	0.45	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000501.3	0.45	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000517.4	0.45	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0001229.4	0.45	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CWS50_RS04105

allorf_0999194_0999376

Leader:

MRENAFDAAGSVAKELSLQEIDERAAQTAGG

Core:

LWTVPTTSTIGLVCTLSWECTRPPRLCGS

Core with dehydrations:

LWDhbVPDhbDhbDhaDhbIGLVCysDhbLDhaWECysDhbRPPRLCysGDha

antiSMASH-DB 4.0 matches for allorf_0999194_0999376

100.0%

NZ_CP025227.1:

Lanthipeptides: allorf_0999194_0999376_lanthipeptide

1

LWTVPTTSTIGLVCTLSWECTRPPRLCGS

29

LWTVPTTSTIGLVCTLSWECTRPPRLCGS

1

LWTVPTTSTIGLVCTLSWECTRPPRLCGS

29

67.7%

NZ_QGGR01000002.1:

Lanthipeptides: BC793_RS05490_lanthipeptide

1

LWTVPTTSTIGLVCTLSWECTRPPRLCGS

29

L+TVP TSTIG +CT+SWEC+ P RLCGS

1

LYTVPGTSTIGWICTVSWECSVPKRLCGS

29

64.5%

NZ_PVMZ01000043.1and 1 other:

NZ_PVMZ01000043.1 Lanthipeptides: CLV67_RS49720_lanthipeptide

Lanthipeptides: CLV67_RS49720_lanthipeptide

1

LWTVPTTSTIGLVCTLSWECTRPPRLCG

28

L+TVP TSTIG VCT S+EC+ P RLCG

1

LFTVPGTSTIGYVCTYSYECSFPKRLCG

28

with 57 more sequence matches

Only the first 10 are displayed here.

55.2%

NZ_CP035495.1and 1 other:

NZ_CP035495.1 Lanthipeptides: ET495_RS17535_lanthipeptide

Lanthipeptides: ET495_RS17535_lanthipeptide

1

LWTVPTTSTIGLVCTLSWEC

20

LWT+P TSTIG CTLSWEC

1

LWTIPGTSTIGRFCTLSWEC

20

55.2%

NZ_LR027842.1:

Lanthipeptides: E0E39_RS15855_lanthipeptide

1

LWTVPTTSTIGLVCTLSWEC

20

LWTVP T TIG VCT+SWEC

1

LWTVPGTRTIGAVCTVSWEC

20

44.4%

NZ_JAPVBQ010000001.1:

Lanthipeptides: O2V63_RS05740_lanthipeptide

1

LWTVPTTSTIGLVCTLSWECTRPPRLCG

28

L+T TT T+G VCT+SWEC+ R+CG

8

LFTFKTTKTVGAVCTISWECS--GRICG

33

38.2%

NC_014643.1and 2 others:

NZ_KV795763.1 Lanthipeptides: HMPREF2554_RS11600_lanthipeptideNZ_KV804818.1 Lanthipeptides: HMPREF2804_RS11135_lanthipeptide

Lanthipeptides: HMPREF0733_RS11155_lanthipeptide

1

LWTVPTTSTIGLVCTLSWECT

21

L+TVP T+T+G CT+SWEC+

8

LFTVPGTTTVGHFCTISWECS

28

37.5%

NZ_CP051884.1and 2 others:

NZ_CP051884.1 Lanthipeptides: HGK68_RS15160_lanthipeptideNZ_CP051884.1 Lanthipeptides: HGK68_RS15160_lanthipeptide

Lanthipeptides: HGK68_RS15160_lanthipeptide

1

LWTVPTTSTIGLVCTLSWEC

20

+WT+P T TIG CT+S+EC

5

IWTIPGTKTIGAFCTISYEC

24

34.5%

NZ_BORO01000007.1:

Lanthipeptides: KI343_RS07745_lanthipeptide

2

WTVPTTSTIGLVCTLSWEC

20

WT+P G VCTL+ EC

9

WTLPGG---GGVCTLTSEC

24

32.3%

NZ_CP035758.1:

Lanthipeptides: EPA93_RS48405_lanthipeptide

4

VPTTSTIGLVCTLSWECTRPPRLCGS

29

+P T G CT + CT P CG+

1

IPDAYTWGNTCTGHYLCTTEPCECGA

26

31.2%

NZ_GL732493.1:

Lanthipeptides: HMPREF0851_RS16015_lanthipeptide

11

GLVCTLSWECTRPPRLCG

28

G V TLS+EC P CG

15

GGVFTLSYECATPHVSCG

32

31.0%

NZ_CP006910.1:

Lanthipeptides: W903_RS05315_lanthipeptide

11

GLVCTLSWECTRPPRLCG

28

G + T S+ECT P CG

2

GWILTQSYECTTPNVRCG

19

31.0%

NZ_CP025249.1and 1 other:

NZ_CP025249.1 Lanthipeptides: CWT12_RS00385_lanthipeptide

Lanthipeptides: CWT12_RS00385_lanthipeptide

2

WTVPTTSTIGLVCTLSWECTR

22

W G VCTL+ EC R

4

WLSRKLGNTGYVCTLTKECQR

24

MIBiG 4.0 matches for allorf_0999194_0999376

20.7%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

11

GLVCTLSWEC

20

G CTL+ EC

14

GAYCTLTVEC

23

20.7%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

8

STIGLVC

14

STI LVC

16

STICLVC

22

16.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

14

CTLSWEC

20

CTL+ EC

19

CTLTKEC

25

with 1 more sequence matches

15.4%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

14

CTLSWEC

20

CTLS EC

28

CTLSKEC

34

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (6):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.07 out of 21.84

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.57 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.90 out of 20.01

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.04 out of 27.04

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.86 out of 36.53

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.04 out of 39.62

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_0999194_0999376 - Class II

Cleavage pHMM score: -2.10

RODEO score: 0

Monoisotopic mass: 3014.6 Da

Molecular weight: 3016.5 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3034.5 Da N = 1; 3052.6 Da N = 2; 3070.6 Da N = 3; 3088.6 Da N = 4; 3106.6 Da N = 5; 3124.6 Da N = 6

TFBS definitions

Regulator	Description

NZ_CP025227.1 - Region 6 - linaridin

Download region GenBank file

Download region SVG

Location: 1,150,801 - 1,177,227 nt. (total: 26,427 nt) Show pHMM detection rules used

linaridin: (cypemycin or cypI)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

CWS50_RS04735: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: methyltransferase

CWS50_RS04765: methyltransferase

smcogs: SMCOG1248: methyltransferase

Isomerases: isomerase

CWS50_RS04730: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000583.5	0.59	ribosomal	grisemycin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000582.4	0.57	ribosomal	cypemycin	Streptomyces sp. OH-4156
BGC0002319.3	0.56	ribosomal	salinipeptin A, salinipeptin B, salinipeptin C, salinipeptin D	Streptomyces sp.
BGC0001188.3	0.48	ribosomal	legonaridin	Streptomyces sp. CT34
BGC0002303.3	0.45	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0001735.5	0.45	other (other)	pentostatine, vidarabine	Streptomyces antibioticus
BGC0002635.2	0.42	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.42	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0002031.3	0.42	other (other)	kitasetaline	Kitasatospora setae KM-6054

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000583.5	0.59	ribosomal	grisemycin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000582.4	0.57	ribosomal	cypemycin	Streptomyces sp. OH-4156
BGC0002319.3	0.56	ribosomal	salinipeptin A, salinipeptin B, salinipeptin C, salinipeptin D	Streptomyces sp.
BGC0001188.3	0.48	ribosomal	legonaridin	Streptomyces sp. CT34
BGC0002303.3	0.45	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0001735.5	0.45	other (other)	pentostatine, vidarabine	Streptomyces antibioticus
BGC0002635.2	0.42	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.42	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0002031.3	0.42	other (other)	kitasetaline	Kitasatospora setae KM-6054

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 25.31

Confidence: medium

Additional weak hits (7):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.29 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.96 out of 29.38

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.11 out of 33.73

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.21 out of 30.97

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.45 out of 36.53

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.83 out of 26.12

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor

NZ_CP025227.1 - Region 7 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 1,261,522 - 1,282,676 nt. (total: 21,155 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

CWS50_RS05250: acyltransferase

smcogs: SMCOG1141: acyltransferase

Ligases: synthetase

CWS50_RS05270: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.38	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002309.2	0.37	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001911.3	0.35	terpene	raimonol	Streptomyces anulatus
BGC0001456.3	0.34	terpene	isorenieratene	Streptomyces argillaceus
BGC0001910.4	0.33	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001483.4	0.33	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0000664.5	0.33	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000936.5	0.32	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0001227.3	0.32	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.31	terpene	carotenoid	Streptomyces avermitilis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.38	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002309.2	0.37	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001911.3	0.35	terpene	raimonol	Streptomyces anulatus
BGC0001456.3	0.34	terpene	isorenieratene	Streptomyces argillaceus
BGC0001910.4	0.33	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001483.4	0.33	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0000664.5	0.33	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000936.5	0.32	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0001227.3	0.32	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.31	terpene	carotenoid	Streptomyces avermitilis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

CWS50_RS05270: PT_FPPS_like

Domain 1 (79 - 371): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.19 out of 23.79

Confidence: medium

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.95 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.37 out of 24.78

Confidence: medium

Additional weak hits (3):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.91 out of 21.55

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 26.85

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response

NZ_CP025227.1 - Region 8 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 1,794,934 - 1,818,263 nt. (total: 23,330 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: phosphotransferase

CWS50_RS07480: Glucose-specific phosphotransferase enzyme IIA

smcogs: SMCOG1145: Glucose-specific phosphotransferase enzyme IIA

Lyases: cyclase

CWS50_RS07460: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.50	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002698.2	0.50	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0001210.5	0.49	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.49	ribosomal	thuricin	Bacillus thuringiensis
BGC0000539.5	0.47	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0001576.3	0.47	ribosomal	divamide A	Prochloron didemni
BGC0000527.5	0.46	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000551.5	0.46	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000528.5	0.45	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.50	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002698.2	0.50	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0001210.5	0.49	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.49	ribosomal	thuricin	Bacillus thuringiensis
BGC0000539.5	0.47	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0001576.3	0.47	ribosomal	divamide A	Prochloron didemni
BGC0000527.5	0.46	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000551.5	0.46	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000528.5	0.45	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CWS50_RS07460

allorf_1797197_1797325

Leader:

MRPPGRHGELCVSPGG

Core:

PEVLPGAVRVVSSFWGRSGSCFPSRG

Core with dehydrations:

PEVLPGAVRVVDhaDhaFWGRDhaGDhaCysFPDhaRG

antiSMASH-DB 4.0 matches for allorf_1797197_1797325

100.0%

NZ_CP025227.1:

Lanthipeptides: allorf_1797197_1797325_lanthipeptide

1

PEVLPGAVRVVSSFWGRSGSCFPSRG

26

PEVLPGAVRVVSSFWGRSGSCFPSRG

1

PEVLPGAVRVVSSFWGRSGSCFPSRG

26

23.1%

NZ_MSTN01000008.1:

Lanthipeptides: BVF97_RS19710_lanthipeptide

1

PEVLPGAVRVVSSFWGRSGSC

21

P PG ++S+ W C

5

PYSTPGCAMLLSAVWATITKC

25

11.8%

NZ_LMHM01000013.1:

Lanthipeptides: allorf_284297_284530_lanthipeptide

11

VSSFWGRSGSCFPSRG

26

VSS GR+ +C P R

37

VSSSTGRAAACPPCRN

52

with 1 more sequence matches

10.8%

NC_016791.1and 1 other:

NC_016791.1 Lanthipeptides: CLO1100_RS15705_lanthipeptide

Lanthipeptides: CLO1100_RS14715_lanthipeptide

11

VSSFWGRSGSCFPS

24

+ + + +G+C PS

7

IVTIYSLTGTCLPS

20

allorf_1797253_1797357

Leader:

MRQAAAGA

Core:

QSWCAPRGGTVSSASARAVPRSCPVL

Core with dehydrations:

QDhaWCysAPRGGDhbVDhaDhaADhaARAVPRDhaCysPVL

antiSMASH-DB 4.0 matches for allorf_1797253_1797357

100.0%

NZ_CP025227.1:

Lanthipeptides: allorf_1797253_1797357_lanthipeptide

1

QSWCAPRGGTVSSASARAVPRSCPVL

26

QSWCAPRGGTVSSASARAVPRSCPVL

1

QSWCAPRGGTVSSASARAVPRSCPVL

26

32.3%

NZ_QLLX01000002.1and 1 other:

NZ_QLLX01000002.1 Lanthipeptides: allorf_335035_335211_lanthipeptide

Lanthipeptides: allorf_335035_335211_lanthipeptide

7

RGGTVSSASARAVP-RSCPVL

26

RG ++A+ R+VP R+CP L

5

RGARRTAAAHRSVPTRTCPYL

25

30.8%

NZ_KB891308.1and 2 others:

NZ_KZ819159.1 Lanthipeptides: DBP19_RS30055_lanthipeptideNZ_RCOL01000001.1 Lanthipeptides: STAN_RS32200_lanthipeptide

Lanthipeptides: B061_RS37915_lanthipeptide

9

GTVSSASARAVPRSCPV

25

GT +S S+ A+ CPV

5

GTYTSPSSYAIGTRCPV

21

with 3 more sequence matches

24.2%

NC_011900.1and 2 others:

NZ_NCVD01000002.1 Lanthipeptides: B7702_RS00350_lanthipeptideNZ_NCVL01000015.1 Lanthipeptides: B7694_RS04515_lanthipeptide

Lanthipeptides: SPN23F_RS06435_lanthipeptide

1

QSWCAPRGGTVSSASARAVPRSCPVL

26

+S+CA G S + CP L

3

RSYCACYSGEHSCGRGCTITTECPFL

28

24.2%

NZ_JVCZ01000028.1:

Lanthipeptides: ADG88_RS11420_lanthipeptide

1

QSWCAPRGGTVSSASARAVPRSCPVL

26

+S+CA G S + CP L

3

RSYCACYSGENSCGRGCTITTECPFL

28

13.9%

NZ_CP077726.1:

Lanthipeptides: allorf_54450_54584_lanthipeptide

19

VPRSCP

24

VPR+CP

5

VPRTCP

10

allorf_1808967_1809146

Leader:

MRFVWLSLGFLVFRGSSCVTYYSTPLTLEMRMCQTM

Core:

LCRYSLRQLQQQGVLWHCSQAPP

Core with dehydrations:

LCysRYDhaLRQLQQQGVLWHCysDhaQAPP

antiSMASH-DB 4.0 matches for allorf_1808967_1809146

100.0%

NZ_CP025227.1:

Lanthipeptides: allorf_1808967_1809146_lanthipeptide

1

LCRYSLRQLQQQGVLWHCSQAPP

23

LCRYSLRQLQQQGVLWHCSQAPP

1

LCRYSLRQLQQQGVLWHCSQAPP

23

21.1%

NZ_QLTT01000004.1:

Lanthipeptides: allorf_749714_749881_lanthipeptide

5

SLRQLQQQGVLWHCSQAPP

23

LR+ G CS APP

8

GLRRDLNMGDRTPCSAAPP

26

17.2%

NZ_CP021707.1:

Lanthipeptides: allorf_3956612_3956845_lanthipeptide

16

WHCSQAP

22

W CS+AP

6

WRCSEAP

12

with 2 more sequence matches

14.3%

NZ_BMUU01000006.1and 2 others:

NZ_BMUZ01000004.1 Lanthipeptides: allorf_271111_271338_lanthipeptideNZ_CP070373.1 Lanthipeptides: allorf_3539637_3539864_lanthipeptide

Lanthipeptides: allorf_274142_274369_lanthipeptide

2

CRYSLRQLQQQGVLWHCSQAPP

23

CR S+ G APP

7

CRASIMPPPDSGAFAGIGSAPP

28

10.0%

NZ_CP070496.1:

Lanthipeptides: allorf_3786998_3787234_lanthipeptide

11

QQGVLWHCSQAPP

23

+ +LW C +PP

5

RGALLWRCGSSPP

17

allorf_1814414_1814497

Leader:

MSRAEVPAM

Core:

ARGVVVPCPGVVAGSRRT

Core with dehydrations:

ARGVVVPCysPGVVAGDhaRRDhb

antiSMASH-DB 4.0 matches for allorf_1814414_1814497

100.0%

NZ_CP025227.1:

Lanthipeptides: allorf_1814414_1814497_lanthipeptide

1

ARGVVVPCPGVVAGSRRT

18

ARGVVVPCPGVVAGSRRT

1

ARGVVVPCPGVVAGSRRT

18

18.4%

NZ_FOLM01000007.1:

Lanthipeptides: allorf_092524_092751_lanthipeptide

2

RGVVVPCPGVVAGSRR

17

RG V PG A SRR

26

RGAVFAWPGCGAWSRR

41

15.1%

NZ_JANBBD010000039.1:

Lanthipeptides: allorf_14543_14731_lanthipeptide

1

ARGVVVPCPGVVA

13

ARGV+ P PG A

9

ARGVLKPDPGGQA

21

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (9):

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.47 out of 29.51

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.88 out of 32.74

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.09 out of 23.43

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.14 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.13 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.79 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.07 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.37 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.60 out of 28.19

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_1797197_1797325 - Class II

Cleavage pHMM score: -1.30

RODEO score: 0

Monoisotopic mass: 2597.4 Da

Molecular weight: 2599.0 Da

Number of bridges: 0

Additional modifications: AviCys

Alternative weights: (assuming N unmodified Ser/Thr residues); 2617.0 Da N = 1; 2635.0 Da N = 2; 2653.0 Da N = 3; 2671.1 Da N = 4

allorf_1797253_1797357 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 2488.3 Da

Molecular weight: 2489.9 Da

Number of bridges: 1

Additional modifications: AviCys

Alternative weights: (assuming N unmodified Ser/Thr residues); 2507.9 Da N = 1; 2525.9 Da N = 2; 2543.9 Da N = 3; 2562.0 Da N = 4

allorf_1808967_1809146 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 2628.4 Da

Molecular weight: 2630.1 Da

Number of bridges: 1

Additional modifications: AviCys

allorf_1814414_1814497 - Class II

Cleavage pHMM score: -3.90

RODEO score: 0

Monoisotopic mass: 1698.0 Da

Molecular weight: 1699.1 Da

Number of bridges: 0

Additional modifications: AviCys

Alternative weights: (assuming N unmodified Ser/Thr residues); 1717.1 Da N = 1

TFBS definitions

Regulator	Description

NZ_CP025227.1 - Region 9 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 1,834,353 - 1,855,411 nt. (total: 21,059 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

CWS50_RS13490: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

Ligases: synthetase

CWS50_RS07600: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002306.2	0.44	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001638.4	0.40	ribosomal	klebsazolicin	Klebsiella pneumoniae subsp. ozaenae
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000504.5	0.40	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0002382.3	0.39	ribosomal	grimoviridin	Grimontia marina
BGC0000665.5	0.39	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000500.5	0.39	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000663.5	0.39	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0001594.3	0.38	other (other)	fischerindole L	Fischerella muscicola UTEX 1829
BGC0002218.2	0.38	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002306.2	0.44	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001638.4	0.40	ribosomal	klebsazolicin	Klebsiella pneumoniae subsp. ozaenae
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000504.5	0.40	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0002382.3	0.39	ribosomal	grimoviridin	Grimontia marina
BGC0000665.5	0.39	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000500.5	0.39	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000663.5	0.39	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0001594.3	0.38	other (other)	fischerindole L	Fischerella muscicola UTEX 1829
BGC0002218.2	0.38	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

CWS50_RS07600: PT_FPPS_like

Domain 1 (26 - 249): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.43 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.75 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.97 out of 30.98

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_CP025227.1 - Region 10 - NRP-metallophore,NRPS

Download region GenBank file

Download region SVG

Location: 2,570,568 - 2,629,912 nt. (total: 59,345 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

CWS50_RS10850: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CWS50_RS10870: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: transferase

CWS50_RS10885: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: hydrolase

CWS50_RS10820: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: isomerase

CWS50_RS10855: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

CWS50_RS10815: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

CWS50_RS13035: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.74	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000401.5	0.59	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002164.2	0.54	NRPS	peramine	Epichloe festucae
BGC0001185.5	0.53	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000309.5	0.52	NRPS	bacillibactin	Bacillus subtilis subsp. subtilis str. 168
BGC0000417.5	0.52	NRPS	rhodochelin	Rhodococcus jostii RHA1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.67	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000401.5	0.64	NRPS	paenibactin	Paenibacillus elgii B69
BGC0000309.5	0.61	NRPS	bacillibactin	Bacillus subtilis subsp. subtilis str. 168
BGC0002164.2	0.61	NRPS	peramine	Epichloe festucae
BGC0001185.5	0.60	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000417.5	0.59	NRPS	rhodochelin	Rhodococcus jostii RHA1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.00 out of 20.47

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.61 out of 36.53

Confidence: medium

Additional weak hits (14):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.38 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.41 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.53 out of 32.74

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.27 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.95 out of 31.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.76 out of 29.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.74 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.85 out of 28.14

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.26 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.11 out of 31.73

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.45 out of 28.19

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.27 out of 30.12

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.80 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 10, location 2570567 - 2629912:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CWS50_RS10815: X - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (570..1004): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YAFSFDSALSPADLFIGGDANGYGPTENTVEATV
Nearest Stachelhaus code(s):: Lys DVSDAGGVEK (47% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1799..2273): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LTTHFDFSVWEGSQVLGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWSVGMVHK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

CWS50_RS13035: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (60..531): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SASLFAMSSAGGALHVGGSQQVFGMAEGLVCYTR
Nearest Stachelhaus code(s):: diOH-Bz AMPAQGVVNK (79% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP025227.1 - Region 11 - phosphoglycolipid

Download region GenBank file

Download region SVG

Location: 2,689,666 - 2,711,566 nt. (total: 21,901 nt) Show pHMM detection rules used

phosphoglycolipid: (MoeO5 or moeGT)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

CWS50_RS11120: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

CWS50_RS11165: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000820.5	0.48	other (other)	physostigmine	Streptomyces griseofuscus
BGC0000846.5	0.42	other (other)	tabtoxin	Pseudomonas syringae
BGC0001395.5	0.40	other (other)	teichomycin A1	Actinoplanes teichomyceticus
BGC0001151.5	0.35	other (non-nrp beta-lactam)	valclavam, (-)-2-(2-hydroxyethyl)clavam	Streptomyces antibioticus
BGC0001374.5	0.35	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001610.5	0.35	other (other)	gougerotin	Streptomyces ahygroscopicus subsp. wuzhouensis
BGC0001739.3	0.34	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525
BGC0000254.5	0.33	PKS	oxytetracycline	Streptomyces rimosus
BGC0000864.3	0.33	other (other)	eicosapentaenoic acid-like compound	Moritella marina ATCC 15381
BGC0000781.3	0.32	saccharide	O-antigen	Pseudomonas aeruginosa

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000820.5	0.48	other (other)	physostigmine	Streptomyces griseofuscus
BGC0000846.5	0.42	other (other)	tabtoxin	Pseudomonas syringae
BGC0001395.5	0.40	other (other)	teichomycin A1	Actinoplanes teichomyceticus
BGC0001151.5	0.35	other (non-nrp beta-lactam)	valclavam, (-)-2-(2-hydroxyethyl)clavam	Streptomyces antibioticus
BGC0001374.5	0.35	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001610.5	0.35	other (other)	gougerotin	Streptomyces ahygroscopicus subsp. wuzhouensis
BGC0001739.3	0.34	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525
BGC0000254.5	0.33	PKS	oxytetracycline	Streptomyces rimosus
BGC0000864.3	0.33	other (other)	eicosapentaenoic acid-like compound	Moritella marina ATCC 15381
BGC0000781.3	0.32	saccharide	O-antigen	Pseudomonas aeruginosa

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 22.20 out of 27.18

Confidence: medium

Additional weak hits (11):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.73 out of 29.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.80 out of 30.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 20.18 out of 30.97

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.96 out of 28.14

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.03 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.10 out of 26.06

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.75 out of 33.73

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.55 out of 32.93

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.92 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.72 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
NrdR	Represses ribonucleotide reductase encoding genes