LOCUS       NZ_CP025227            21059 bp    DNA     circular CON 14-APR-2024
DEFINITION  Actinomyces wuliandei strain 299 chromosome, complete genome.
ACCESSION   NZ_CP025227
VERSION     NZ_CP025227.1
KEYWORDS    RefSeq.
SOURCE      Actinomyces wuliandei
  ORGANISM  Actinomyces wuliandei
            Bacteria; Actinomycetota; Actinomycetes; Actinomycetales;
            Actinomycetaceae; Actinomyces.
REFERENCE   1  (bases 1 to 3107761)
  AUTHORS   Zhu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2017) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No.155 ChangBai Road, Changping District, beijing
            102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. HGAP 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004010955.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 19:14:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,606
            CDSs (total)                      :: 2,544
            Genes (coding)                    :: 2,496
            CDSs (with protein)               :: 2,496
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 18 of 48
            Pseudo Genes (incomplete)         :: 37 of 48
            Pseudo Genes (internal stop)      :: 3 of 48
            Pseudo Genes (multiple problems)  :: 9 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:15:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1834352
            Orig. end                         :: 1855411
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21059
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="9"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21059
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21059
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11059](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11059)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            195..1217
                     /locus_tag="CWS50_RS07565"
     CDS             195..1217
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009407009.1"
                     /locus_tag="CWS50_RS07565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Tat pathway signal protein"
                     /protein_id="WP_243118234.1"
                     /transl_table=11
                     /translation="MTSPLTPLPRLAPASRHLTGRAAGARLVRAAAPLLVMLLATLLSG
                     CGLRLGEGTPASLPQPSAQESVRDALARHTWLISSSAAVVARSGDRQAEAAQGVETVAG
                     SQLDALGGVWEPWASEVPTRFPTISPAATAAATATSEDLAALLSEGAALAQEAAVSAPS
                     GQEAQLYASLAASWSIQHETVSPGSTTSVPRSSLDTPQQVSSDLLLAYDAARYACQEVA
                     ARAQAATRERAAQDAGSASAAVSAALAVGSEDTRLAAYASPSQSPDASAADTDVLWAQT
                     VWSDVVDHEAQEAASTQAGSPARQAAVEATTDAALRAHAWGAQLSSLPGYLEPSESESS
                     "
     gene            complement(1239..3059)
                     /locus_tag="CWS50_RS07570"
     CDS             complement(1239..3059)
                     /EC_number="6.1.1.15"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /GO_function="GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /GO_process="GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1162: threonyl-tRNA
                     synthetase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017194998.1"
                     /locus_tag="CWS50_RS07570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline--tRNA ligase"
                     /protein_id="WP_127842296.1"
                     /transl_table=11
                     /translation="MLQTMSTAFIRTLREDPADADVASHRLLLRACYIRRAAPGVYTWL
                     PLGLRTLRKIERVVREEMDAIGAQEVHFPALLPAEPYKATGRWQDYGPTLFRLRDRKDA
                     DYLLAPTHEEMFTLAVKDLYSSYKDLPAVIYQVQTKYRDEARPRAGIIRGREFVMKDSY
                     SFDLDEEGLEASYQAHREAYDRVFTRLGLDYVIVNAVAGAMGGSRSEEFLHPSPIGEDT
                     FVRTDGGYAANAEAVTTPVPEAVDASRTGPARVVDTPDASTIEALVAVCNDLYPREDGR
                     AWTAADTLKNVVVVATHPSGERELLVVGVPGDRDVDLRRLEAALAPAQVEMASDQDFEG
                     HPELVRGYIGPPAVGPSSPLRRVETAEDGSQRTVGSVRYLVDPRVVEGSAWVTGANQPR
                     KHVLDLVMGRDFEVDGTVEAAEVREGDPAPDGSGPLQLARGIEVGHIFALGRKYSQALG
                     LSVLDEDGRARVVTMGSYGIGVSRVMAALAEAHHDERGLTWPAVVAPYHVQVLATGKGE
                     EVFAVAAQIAADLDDAGVEVLYDDRRKVSAGVKFADAELLGLPYTLVVGRDLGREGTVE
                     IRDRRSGERSSVLASEAAATLVRLVRAALA"
     gene            3280..3540
                     /locus_tag="CWS50_RS07575"
     CDS             3280..3540
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CWS50_RS07575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_127842297.1"
                     /transl_table=11
                     /translation="MKDVMTIMDSSSPAYSRLREGHGLAETRAFLLDEHSVADGTAFIA
                     VENQRRVSQGRSDVMVSGCTAEQTRLLGADLVRLARRSSGE"
     misc_feature    3293..3307
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.75"
                     /tool="antismash"
     gene            complement(3768..6215)
                     /locus_tag="CWS50_RS07580"
     CDS             complement(3768..6215)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /locus_tag="CWS50_RS07580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FtsX-like permease family protein"
                     /protein_id="WP_127842298.1"
                     /transl_table=11
                     /translation="MLGIRLFRLTRRSLATSVWPQLFQSLVLLTNVALIVGATLVWSSL
                     TRGSQDFVTQLASGADTVVVAPSGQGLTSSEIERISQAEGVDSALCASWVEGEVDGSST
                     VLIGLRSLGEPVSALGETEDDLGLVGLGHRAARSGTGGLRLTSGLRDIDIDPDDVSVVD
                     TEAARAVNSGNFVVLPLDLLAGISSQQPQAVFLTRSPQASADSLKESLHQVLGEEYSVM
                     SVVQYAELVSRQYQLMATVSAVFTAGALLGGMLLIYISSLIEVRRKQHQYSVLRMLGVR
                     RAAIASWSLGEQALVLLPALVAGGWLGVGLAGALLDWLPPAVTSGLPITPTLRIAWYQV
                     GALLAAAYLAAVLGRAYATRKVLGDVDSGLVYLRSGQAGLLTTPRRAVVVGAAIALCCA
                     AGGAALPLLRGTLLGTAALWLVPACLVGLARLCRSAGLGLRGVAGLSLLGASRRLRGNV
                     VLAVLVAAVNGMLVASTAPALALVERGNQSVASLLRNDYYLQSAPADQLPTSGFLSYQD
                     ALGLVDAPAIKQVSPGVLMTTSLASGQFTIQGVSAGSAMPAAYNAGAQKVRDLSDTRGL
                     TIISRKAAASLDLAVGDQLHLPIGRGLDLTVTAVADYIGLDDGQIVVSMRDLCEVQDLC
                     QVSYVEIRSDGQSALTKDDLGDLDYLIPEDQVHLNTAEAENQALGASIASTSALSVMLG
                     LQLVAAAAAGIFGVYRNEIDSRRPTLTSLGRMGLSVRQVLVAQLGQHLVLGVCASALGG
                     FVGALWAGGLTVFLENGLALRGIGAELTWWMITASAGTGLVVLTSAAVAGMATGLRSVA
                     LRG"
     misc_feature    complement(5451..5453)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6221..6892)
                     /locus_tag="CWS50_RS07585"
     CDS             complement(6221..6892)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="CWS50_RS07585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_127842299.1"
                     /transl_table=11
                     /translation="MIELRDVSKTFRDGQNHSHHVFESLTHVFGDDVASTAILGESGCG
                     KSTLLSLLGLLSRPDSGCVVLSGTDVTAMPDSRRGEYRLRNIGMVFQEFNLVPELCLWE
                     NVALPSVALGGSFAARRSRALDLLARVGCDGLAERLPSQVSGGQMQRVAVARALVNRPA
                     LILADEPTGNLDPKTGSLVVEALTEAARSTRLLIVTHDAAVARRMDEVLVLSDGGLRRQ
                     D"
     gene            complement(6894..8270)
                     /locus_tag="CWS50_RS13490"
     CDS             complement(6894..8270)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /locus_tag="CWS50_RS13490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="WP_243118235.1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.4e-11, bitscore:
                     41.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSRSVFSLLSAANGIYDIAFDGGDVEIEVDGVGSDRVRQYFPAL
                     RSRSRRYSAGSDFINLGALSVALSSSRRPVIVAGSGSIPCADKVMQASGQAGIPVLCTM
                     GSLWRYASHKHCLGMIGSSGSVECERIRSHSDLVIYLGLTNRGLAHDTLRLDRPYFDVC
                     EDPSVLAGRSDPGRQVCGSIGDVLDRLRCSLPSPERDHYWFRLLHDAAVQFSRPRARLT
                     ERMSAARPSSRQTVRAVVSQYAPAETMIFVDVGLSTLWASRTLPRGYKVLYTRGFATMG
                     AATLSALGVSDCLTRRLLVITGDGGFALSLGEFLHHAAGTASGAGGGRSSVQPDLTVLV
                     LNNGGLGAVRYEQELRGLVPSAWAAPFNAPALAALLPAIEYVSVTSRADLERMDQGRAT
                     TRRVRVINAHVDPLEAPVPARRIDIRARTTLIRSWARQGRQGLFHAARTLPYIIDTWRF
                     "
     misc_feature    complement(8067..8069)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9138..9154)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.97"
                     /tool="antismash"
     gene            complement(9367..10086)
                     /locus_tag="CWS50_RS07595"
     CDS             complement(9367..10086)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /locus_tag="CWS50_RS07595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_164860104.1"
                     /transl_table=11
                     /translation="MTVSSESAETAGRPTTPQRNFTAVARRYDSINRILTCGRITVARR
                     KAIDSLPTTSNKFLDLCCGTGEFGRALRLRYPGSTIIGVDINRSMLAQAVSRDCYDLVL
                     AQDVATLELPPGSFNCVVLSLAYHDLGDGGEQVLAMSGRLLAPAGRLVSLELSLPESPL
                     MRWLLVRVLRALESLLTILSRPVLAHTVREIRMSPDWRSVVHEFEAAGFSLMSTTTFLL
                     GVLRLNIYIKGRTHDPA"
     misc_feature    complement(9619..9621)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9625..9627)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(10001..11059)
                     /locus_tag="CWS50_RS07600"
     CDS             complement(10001..11059)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /locus_tag="CWS50_RS07600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_127842302.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.6e-45, bitscore:
                     152.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSSLEEEIVEHISRAISTMPSSVGARAAYSCEVPGKLVRPRLLV
                     CTAAGLGLQPDDSGLLDVAEAIEMGHVASLIHDDIIDGSPLRRGRSALHTKFGAATAII
                     TGDAMLFSMFRGLSSACPPDVATQIVRTIADMGTRLCEGEHLEEAATRSATTAVRSQEA
                     LSLATYMRVIRNKTASYFVAMCDIAVAMAPGGFEARDDLLKMAELFGMAFQVRDDLLRV
                     LPSSVPAGKPLGIDESNGLVTAFDLHGAGFDLFEAVREYVNNGGVEETSAAVRPRREVQ
                     RPQAQEVDILLKKFRRELVQRVDALPCHAKGAGCLSRISSGRHCDSVVRISRDGRSADD
                     AAAQLHRRRPPL"
     gene            complement(11043..12050)
                     /locus_tag="CWS50_RS07605"
     CDS             complement(11043..12050)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /locus_tag="CWS50_RS07605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="UbiA family prenyltransferase"
                     /protein_id="WP_127842303.1"
                     /transl_table=11
                     /translation="MAKRFAGRATAVTLVALSRPRTCFVSVFSLLLAFSTTGISWTWRL
                     LSALIASYLVPAVANLHNATTDVTEDSYHLSNRVLAVRRLGSTRVTVVLVSSTGVLALL
                     LAATSLATLAVAPVGIGLMYMYSMPPFRLKSQPLAGACTFALVAALPYIGGSLVSNRWA
                     TIGGSQLRLWPTALLALVMCGKFFIKNLPDFDADKRAGLKATATIFTSYRNAAVTAVLF
                     EAGAYVAASVYVFASTASTLKIIMHWVVLLLCVGHATSFVRTFDALALNARMKWDMLAT
                     TLFVASWAFPAEAGYHMNLWLLSMLGLATAMIVPLDSRAGWYISRTSERHEQQS"
     misc_feature    complement(11325..11327)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(12306..12863)
                     /locus_tag="CWS50_RS07610"
     CDS             complement(12306..12863)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /locus_tag="CWS50_RS07610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="STM3941 family protein"
                     /protein_id="WP_127842304.1"
                     /transl_table=11
                     /translation="MEPLVIKHKSSYILPRVAGSLVLVIASIVVLAIEPPRVLVAMLGS
                     GSARLTCVAVGLLGIVFFSYTAVRTARLGRGHALRVDDAGIDNRTNAVCVRRVPWEQVA
                     GFRPLRVRMGIQDIDSVQIDFVDPQWPRTQISGLKAATVRANEGMAGFGPGSISTDPLG
                     VTSEELHARLSEELRRRRPDPR"
     gene            complement(13033..14022)
                     /locus_tag="CWS50_RS07615"
     CDS             complement(13033..14022)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017176998.1"
                     /locus_tag="CWS50_RS07615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_127842305.1"
                     /transl_table=11
                     /translation="MTLVSRLRRRGPEGTGLVPVAADRVGGLLGLCGTEPVQATPLAHQ
                     VQRWSRWGRGDVVVAGDPQAPVGAAWTTGVAMPFGLAPRPGTGHGGVARSTLLALAEHT
                     RPRLGRHGSVVGVARDVAALWEPLRRLGAVPREERWNQPLLRAPASPGGEGLVAGQRRR
                     RPALGWVAQGLHAATRREEPLVMPASVAMFTAEVGYDPTTTGSSYARHVSWLVSTGRSY
                     VVLDDGTGAPARPGSAAMVAFKADVGSVWHSARDHVAMLTGVWTRPDLRGRGVGAVATA
                     AVVDAVRRDHVGPQGTVSLYVNDFNAPALALYCSVGFTQVDTFATVLL"
     gene            complement(14118..15299)
                     /gene="ispG"
                     /gene_synonym="gcpE"
                     /locus_tag="CWS50_RS07620"
     CDS             complement(14118..15299)
                     /EC_number="1.17.7.1"
                     /GO_function="GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA]"
                     /GO_process="GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ispG"
                     /gene_synonym="gcpE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786387.1"
                     /locus_tag="CWS50_RS07620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="WP_127842306.1"
                     /transl_table=11
                     /translation="MSDVIDLGIPSVRAEAPVLAPRRPTRKIRVGGLEVGGDAPVTVQS
                     MTTTKTHDIGATLQQIAELTAAGCDIVRVACPTDKDAAALPVIARQSRIPVIADIHFNP
                     KYVFAAIEAGCGAVRVNPGNIRKFDDRVAEICKAASDHGVSLRIGVNGGSLDPRLLKKY
                     GKATPEALVESATWEAGLFEENDFHDFKISVKHHDVVTMVQAYRLLSQAGQWPLHLGVT
                     EAGPAFQGTIKSCAAFGTLLAEGIGDTIRVSLSAPPVEEVKVGTKLLEFMGLRQRQLEI
                     VSCPSCGRAQVDVWSLAEQVEEGLKGVTAPLRVAVMGCVVNGPGEAREADLGCASGNGK
                     GQIFVRGKVVETVREEQVVETLLRYAQDMAEDMTRELGEEALVGTAPMVSAAG"
     misc_feature    complement(14493..14495)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(15369..16700)
                     /locus_tag="CWS50_RS07625"
     CDS             complement(15369..16700)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M50"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548722.1"
                     /locus_tag="CWS50_RS07625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="site-2 protease family protein"
                     /protein_id="WP_127842307.1"
                     /sec_met_domain="Peptidase_M50 (E-value: 1e-42, bitscore:
                     143.4, seeds: 56, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQALAYVLGIAVMVVGIAVSVALHELGHMLPAKKFGVKVPEYFI
                     GFGPRLWSVRRGETEYGVKALWLGGYVKLVGMLPPENPDRPDRRTSSGELGMVGQARAD
                     SMEEIAPGEEDRAFYRLSVPRKLVVMAGGILTNLVLGVVLLAVSLGVVGVPTSTSSLAS
                     VAPCVSSDIDAGAPCTEEDPVSPAARAGLEPGDRIISWNGVPVSTWQQVTAQIERAGTT
                     PTEVVVERDRARRTVSVTAVEVERAVRDDQGSPVTDSTGTALTRSRPYVGLSPASETRR
                     VPLGELPGMVGQAVGGTLRAIATLPVGLYHVVAAGLGLEERSTTGVIGLVGLGRHAGQV
                     SSAGVGEGTVSLSARVSAMLSLLGSLNLALFAFNLVPLLPLDGGHVAGACWEGLRRRWA
                     AARGRPDPGPVDIARLLPVSQVVVALLVVMAVVLVWADLVAPVT"
     gene            complement(16697..17980)
                     /gene="dxr"
                     /locus_tag="CWS50_RS07630"
     CDS             complement(16697..17980)
                     /EC_number="1.1.1.267"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="dxr"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008729352.1"
                     /locus_tag="CWS50_RS07630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="WP_127842308.1"
                     /transl_table=11
                     /translation="MTEPSRLVLLGSTGSIGTQALQVLTRLGDRAPQVAALAAGGSRLD
                     LLAEQAVAWQVPVLAVACQGGEQDAVREHLRQALSRAAACSAVPNPVTTVLTGPQATVD
                     VVEAAAPARNDTVLNAVTGSVGLLPTLAALRSGARLALANKESLVVGGALVRDALRRPG
                     QVVPVDSEHSAIAQCLLSARHEKGLTSPLVTGRSEVRRLVLTASGGPFRGRRREELVGV
                     TAAQALDHPTWSMGPVVTVNSSTMVNKGLELIEAHLLFDVDPGDIDVVVHPQSVVHSMV
                     ELLDGATIAQASPPDMRLPIALGLTWPDREDLSGLVEPTRWEDPVAWTFEPLDSQTFPA
                     VDLARQAVSASDTHPAVLNAANEQVVEAFLVGGLEWLDVTSIDAAVLAEHEGLSSPGLD
                     ELLEVESWARARAQEMVEARSGHVGGDA"
     misc_feature    complement(18057..18073)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.43"
                     /tool="antismash"
     gene            complement(18127..18672)
                     /locus_tag="CWS50_RS07635"
     CDS             complement(18127..18672)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786395.1"
                     /locus_tag="CWS50_RS07635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DivIVA domain-containing protein"
                     /protein_id="WP_127842309.1"
                     /transl_table=11
                     /translation="MSNMFPKSMPLRPGYRPEQVDRYFETAREIYDAGELDEMDSEGVR
                     TVAFDIVPGGYQPAAVDAALDRLEAAFLQRRRADFVAQHGRRAWMDQVTQLATTLYPRL
                     LRPEGERFLPAGGQGYAKEDVDALMDRIAGYFDSDNALASSEVRGAVFRAARGSKAYDE
                     ASVDRYLARVVEVLLSVE"
     gene            complement(18669..19964)
                     /gene="rlmN"
                     /locus_tag="CWS50_RS07640"
     CDS             complement(18669..19964)
                     /EC_number="2.1.1.192"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008729353.1"
                     /locus_tag="CWS50_RS07640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="WP_127842310.1"
                     /sec_met_domain="PF04055 (E-value: 7e-18, bitscore: 62.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPASHRPGAPAGRAARHGPADRAPGSASPAGPAPGEVQVLPTDQ
                     PPEGASSPDARPRLSLAVPAPRGRAPRHLADLDLAGRKAACQDAGLPGFRADQVSRHYF
                     IRLTRDPARMTDLPASQRDQLCAELLPPLICEVRALRADAGRTVKHLWELHDGVRVESV
                     LMRYEDRTTLCVSSQAGCGMACPFCATGQMGLTRNLSTAEIVEQVRHAALASARGDLAG
                     GPARLSNVVFMGMGEPMINYRNVVAALHRLVDPAPEGFGMSARGITVSTVGVVPLIRRL
                     AGEGLPVTLAVSLHAPDDALRDELVPVNSRWKVADLLDAAYDYFQASGRRVSIEYALIK
                     DMNDHAWRAQLLAEELNRRGRGWAHVNPIPLNPTPGSIWTCSTPQAQQSFVDTLRCAGI
                     TTTVRDTRGSDIDGACGQLATEVLNQERARTQ"
     gene            complement(20003..20932)
                     /locus_tag="CWS50_RS07645"
     CDS             complement(20003..20932)
                     /EC_number="2.7.7.41"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017177005.1"
                     /locus_tag="CWS50_RS07645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="WP_127842311.1"
                     /transl_table=11
                     /translation="MSTLLTPPPTRDHAPLPATGRAGRNLPAAVGVAVVLVLLVAGSLL
                     FYRELFVLLAVVAVCGAQWEMAGALARKRIRLPLAPLWLGTLGTAVCAWQVGAEAAFGS
                     YVATAGACVLWCFMDQAEADTGTPLEESGNAVPRSDLHDMVRRSRSRSAAASVFAATYL
                     PFLASFAVLVAAQEEGAQRVMMMIALPAASDTGGWLVGVLLGRHPMAPSVSPKKSWEGF
                     VGSLLATVAAGAGLLYLLDGPVWAGAVLGACVVVVSTLGDLGESLLKRDLGLKDMGTLL
                     PGHGGMMDRLDSVLVAAPVVYVFTLVVW"
ORIGIN
        1 caccccctcc agaaagaggc cctcgcgctc gaccaccgga cgcagcagcg cggcaaggtc
       61 atggctgtca tgacggtcct ggctccccct gccgtgactc gtgtctgcca taaggtcagc
      121 tcccttcctg aggcctaccc tctgcgggcg tggtctggct gggatcatgc tacctgccgt
      181 gggaggatcg tcaggtgacg agtcccctga ccccgctgcc ccgcctggct cccgcctccc
      241 gccacctgac aggccgggca gcaggagcac gactggtacg cgccgccgcc cccctcctcg
      301 tgatgctcct cgcgacgctg ctcagcggct gcgggctgcg cctgggagaa ggaacgccag
      361 cgtcgctgcc ccagccctcc gcccaggagt ccgtgcgcga cgcgctggct cggcacacct
      421 ggctcatctc ctccagcgcc gccgtcgtgg cccgctccgg cgaccggcag gccgaggccg
      481 cgcagggggt ggagacggta gcaggctccc agctcgacgc cctgggcggg gtgtgggagc
      541 cgtgggcgtc tgaggtcccg acacgtttcc ccaccatcag cccggcggcc acggctgccg
      601 cgaccgccac cagcgaggac ctggccgcgc tcctcagcga gggcgccgcc ctggcccagg
      661 aggctgccgt gagcgctccc tccggccagg aggcccagct ctacgcgtcg ctggccgcct
      721 cctggagcat ccagcacgag accgtctccc ccggatcaac cacctcggtc ccgcgcagct
      781 cactggacac gccccagcag gtcagcagcg acctgctcct cgcctacgac gcagcccgct
      841 acgcctgcca ggaggtggcc gcgcgcgcgc aggccgccac gcgtgagcgc gccgcccagg
      901 acgccggctc cgcctccgcc gctgtcagtg ccgccctggc tgtcggcagt gaggacacac
      961 gtctggcagc ctacgcgagc ccctcccagt ccccggacgc ctctgcggca gacaccgacg
     1021 tcctgtgggc acagaccgtg tggtcagacg tggttgacca cgaggcccag gaggcggcct
     1081 ccacacaggc agggtccccg gcccggcagg ctgccgtgga ggcgacgacc gacgcggccc
     1141 tgcgcgcaca cgcctggggg gcgcagctgt cctccctgcc cggctacctg gagccctcag
     1201 agtcagagtc gtcctgagaa gccaccctga gaagaggctc acgccagagc ggcgcggacc
     1261 aggcgaacca gcgtggcggc cgcctcactg gccagcaccg agctgcgctc cccgctgcgg
     1321 cggtcacgga tctccacggt cccctcccgc cccaggtcac ggcccacgac cagcgtgtag
     1381 ggaaggccga gcagctcggc gtcggcaaac ttcactccgg cgctgacctt gcgacggtcg
     1441 tcgtagagga cctcgacacc ggcgtcgtcc aggtctgcgg caatctgggc agcgacggcg
     1501 aagacctcct cccccttgcc ggtggccagc acctggacgt ggtagggagc caccacggct
     1561 ggccatgtca ggccacgctc gtcgtggtga gcctcggcca gggcggccat gacccgggag
     1621 accccgatgc cgtaggagcc catggtcacc acgcgcgccc tgccgtcctc gtccagcacg
     1681 ctcagcccca gggcctggga gtacttccgt cccaaggcaa agatgtggcc gacctcaatg
     1741 ccccgggcca gctggagcgg gcccgagccg tcgggagcag ggtcaccctc acgcacctca
     1801 gcggcctcga cagtaccgtc cacctcgaag tcacgcccca tgaccaggtc caggacgtgc
     1861 ttcctgggct ggttggcccc ggtgacccag gcgctgccct ccaccacgcg ggggtccacc
     1921 aggtagcgga ccgagccgac cgtccgctgg ctgccgtcct cggcggtctc cacacggcgc
     1981 agaggcgagc tggggccgac ggccggcgga ccgatgtagc cgcgcaccag ctcggggtgg
     2041 ccctcaaagt cctggtcaga ggccatctcc acctgcgcgg gggccagggc ggcctccagc
     2101 cgcctgaggt cgacgtcgcg gtccccgggc acgcccacga caagcagctc gcgctcgccc
     2161 gaggggtggg tcgccacgac aacgacgttc ttcaaggtgt cggccgcggt ccaggcccgc
     2221 ccgtcctcgc gagggtagag gtcgttgcac accgcgacaa gagcctcgat cgtggaggcg
     2281 tcaggggtgt ccacgacccg ggcagggcct gtccgcgagg cgtcgacggc ctcgggcacc
     2341 ggggtggtga cggcctcggc attggccgcg tacccaccgt cagtgcggac aaaggtgtcc
     2401 tccccgatcg gggaggggtg gaggaactcc tcggagcggg agcctcccat ggccccggcc
     2461 acggcgttga cgatgacgta gtccagaccc aggcgggtga agacgcggtc gtaggcctcg
     2521 cgatgggcct ggtaggaggc ctccaggccc tcctcgtcca ggtcgaagga gtaggagtcc
     2581 ttcatgacga actcgcgtcc gcggatgatc ccggcccgcg ggcgggcctc gtcacggtac
     2641 ttggtctgca cctggtagat gacggcgggc aggtccttgt aggaggagta gaggtccttg
     2701 accgccaggg tgaacatctc ctcgtgggtg ggggccagca ggtagtccgc gtccttgcgg
     2761 tcccgcagcc ggaacagggt ggggccgtag tcctgccagc gtccggtggc cttgtagggc
     2821 tctgcgggca gcagcgccgg aaagtggacc tcctgggcac cgatggcatc catctcctca
     2881 cgcacgacgc gctcaatctt gcgcagggta cgcagcccca gcggcagcca ggtgtagacg
     2941 cccggtgcgg cacgacggat gtagcaggct cgtaggagga gccggtggct ggccacgtcg
     3001 gcgtcggcgg ggtcctcgcg cagggtacgg atgaaggcgg tggacatcgt ctgaagcacg
     3061 ggcacatgct acgtgctccg agcgccgccg acggcctcgc tcaccgggag gcaggcggtc
     3121 caggtgagcg acgtggaagg tcttgccacc gggcagcctt cccgacacgc ccgcgtccga
     3181 cggctcgtcg ctcctcccgc gtgagcctac ctggtcccgg gcacaggagc agcggggacg
     3241 aacgggttcc aggtataccg ggtattttaa ggacacggca tgaaagacgt catgacaatc
     3301 atggacagct cgtcccccgc ttacagtagg ctgcgtgagg ggcacggcct agccgagacc
     3361 agggcctttc tcctggacga gcactccgtg gccgacggga cggccttcat tgccgtggaa
     3421 aaccagagac gcgtcagcca gggccgctcc gacgtcatgg tgagcgggtg caccgccgag
     3481 cagacccgtc ttcttggtgc cgacctggtc aggctcgccc gccgctcgtc gggcgagtaa
     3541 ggcagacacg gctggtggcc gaggagacgg gacaggacgc cccggacggt aggcgggtag
     3601 caggcagatc gggagagagc acgggggcag gacaccccgg acagtaggcg gctagcaggc
     3661 agaccgggag agagcaagga gacgccccga ccctcacccg ctcagtacgc ctggacaggc
     3721 tagcgaggtg gacggaggtc accaaggcgc tggcctggac ccccggccta gcctctcagg
     3781 gcgacggagc gcaggcccgt tgccataccc gcgactgccg cgctggtgag cacgacgaga
     3841 ccggtgccag ccgacgccgt gatcatccac caggtcagct cggcaccgat acccctcagc
     3901 gccagcccgt tctcgagaaa caccgtgagg ccgcctgccc acagggcgcc gacgaacccg
     3961 cccagagctg aggcgcacac acccaaaacg aggtgctgcc caagctgggc caccagtacc
     4021 tgccgcacgg acagccccat cctgcccaga ctggtcaagg tgggtctgcg tgagtcgatc
     4081 tcgttgcggt acacgccgaa tataccggcg gcggccgctg ccacgagctg tagccccagc
     4141 atgaccgaca acgctgaggt tgaggcgatc gaggctccca gcgcctggtt ctcggcctct
     4201 gcggtattga ggtgcacctg gtcctcaggt atcaggtagt cgaggtctcc gagatcatct
     4261 tttgtcagcg cgctctgccc gtctgaccgg atctctacgt aggagacctg gcagaggtcc
     4321 tgaacctcgc agaggtcgcg catagagaca acgatctgtc cgtcatcaag accaatatag
     4381 tcagcaaccg ccgtcacggt aaggtcaagc cctcggccga tcggcaggtg cagctggtcg
     4441 cctactgcga ggtcaagaga ggcggcagcc ttcctgctga taatcgtgag accgcgggtg
     4501 tcactcaggt cgcgcacctt ctgtgccccc gcgttgtagg cggctggcat ggcagagccc
     4561 gcggagactc cttggatggt gaactgtccg cttgccaggg aggtggtcat cagcacgcct
     4621 ggtgagacct gcttgatggc gggcgcatca accagcccca gagcgtcctg ataggacagg
     4681 aagcctgaag tcggcagctg gtcggctggt gcggactgga ggtagtagtc gtttctcagc
     4741 agcgacgcca cagactggtt cccacgttcc accagggcga gagcgggcgc cgtcgatgcc
     4801 acgagcattc cgttgaccgc cgcgacgagc acggccagga ccacgttgcc cctgagcctg
     4861 cgggaggcac ccagcagcga cagccccgcg acccccctga ggccgagccc ggccgagcgg
     4921 cacaggcggg ccagcccgac caggcaggca gggacgagcc acagggccgc ggtccccagg
     4981 agggtgccac gcagcagcgg gagcgccgca ccacctgcgg cgcagcacag ggcgatggca
     5041 gcgcccacga caaccgcccg cctgggagtg gtaagcagac ccgcctgccc agaccgcagg
     5101 tacaccaggc cgctgtcaac gtcaccaagg accttgcggg tggcgtacgc cctgcccagg
     5161 acggcggcaa ggtatgccgc cgccagcagt gcacccacct ggtaccaggc tatgcgcagc
     5221 gtaggggtga tcggcagccc actggtcact gctggaggga gccagtcgag cagggcaccc
     5281 gcaagaccga caccgagcca gcctcccgcg accagcgctg gcagcagcac cagggcctgc
     5341 tcgcccagag accatgacgc tatggcggcc ctgcgcacac caagcatcct caggacggag
     5401 tactggtgct gcttgcgacg tacctcgatg agtgaggaga tgtagatgag taacatcccg
     5461 ccgagcaggg cgccagcagt gaagactgcc gagacggtcg ccatgagctg gtactgccgg
     5521 ctcaccagct ccgcgtactg caccacgctc atcaccgagt actcctcccc cagcacctgg
     5581 tgaaggctct ccttgagtga gtcggcgctg gcttgcggcg agcgcgtgag gaagacagcc
     5641 tgaggctgct gggagctgat acccgccagc aggtcaagcg gcagcacgac aaagttgccg
     5701 ctattgacgg cacgcgccgc ctcggtgtcc accacagaga cgtcgtcagg gtcgatgtca
     5761 atatcccgca ggccgctggt gagccgcagc ccacccgtgc cgctcctggc agcccggtgc
     5821 ccaagaccaa caagtcccag gtcgtcctcg gtctcgccca gcgcggatac cggctcccca
     5881 agactcctca ggcctatcag gacagtagaa ctgccgtcca cctcgccctc gacccaggac
     5941 gcgcacagag cgctgtccac accctccgcc tgggagatcc gttcaatctc gctggaggtc
     6001 aggccctggc cgctgggggc gacgacgacg gtgtcagcac ctgaggcaag ctgtgtgacg
     6061 aagtcctgcg agcctctcgt cagcgacgac cacacaagcg ttgcgccaac gatgagtgcg
     6121 acgttggtca ggagcaccag ggactggaac aactgaggcc acaccgaggt tgcgagggac
     6181 cgccgggtga gtcggaagag acgtatacca agcatgtcgc ttaatcctgt cttctgagcc
     6241 cgccgtcact gagaacaaga acctcgtcca tcctgcgcgc aactgcggcg tcgtgcgtga
     6301 ctatcagcag gcgggtgctg cgggcggcct ccgtgagcgc ctcgaccacc aggctgcccg
     6361 tcttggggtc gaggtttcct gtgggctcgt cggcgaggat gagtgcgggc ctgttgacca
     6421 gggcgcgcgc gaccgcgacc cgctgcatct ggccgcccga gacctgtgac ggcagacgtt
     6481 ctgccaggcc atcacagccc acccgtgcga gtaggtcaag cgcgcggctc ctcctggcag
     6541 cgaaggagcc tccaagcgcg accgagggca gggcgacgtt ctcccacagg cacagctccg
     6601 gcaccaggtt gaactcctgg aagaccatcc ctatgttcct gagccggtac tcgccccgtc
     6661 gtgagtcggg cattgccgtg acgtctgtgc cactgagcac gacgcagcct gagtccggtc
     6721 tgctcagcag cccgagcagc gacagcaggg tagacttccc gcagccgctc tcccccagaa
     6781 tagccgtcga cgcgacgtcg tcaccaaaca cgtgcgtcag gctctcgaag acgtggtgag
     6841 agtggttctg tccgtctcgg aaggtcttgg agacgtcccg cagctcaatc atgtcagaac
     6901 ctccacgtgt cgataatgta gggcagcgtc ctggccgcgt ggaaaagccc ctgccttccc
     6961 tgtctggccc aggagcgaat cagggtcgtg cgggcgcgga tatcaatcct gcgcgcaggg
     7021 accggcgcct ccagcggatc aacatgagcg ttgatcaccc gcacccgacg tgtcgtggcc
     7081 cggccctggt ccatcctctc gagatcggcc cgtgacgtga cggagacata ctcgatcgcc
     7141 gggaggagcg cggcaagggc tggggcattg aatggagcgg cccaagcgct agggacaagg
     7201 cctcgaagct cctgctcgta gcgcaccgcc cccaggccgc cattattgag taccaggact
     7261 gtgaggtcag gctgtaccga agaacggccg cctccagctc ccgaggcagt ccctgcagcg
     7321 tggtgcagga actctcccag gcttagagca aaccctccgt cgccagtgat cacgagcagc
     7381 cgcctagtaa gacagtccga cacgcccaag gcggagagag tggcggctcc catcgtcgcg
     7441 aagccccgcg tatacagcac cttatagccg cgtggcaacg tgcggctggc ccagagggtg
     7501 ctcagcccga cgtcaacaaa gatcatcgtc tcagcagggg catactgcga aacgaccgcc
     7561 cggaccgtct gacgtgaact agggcgtgcg gcgctcatcc tctcagtaag ccgtgcccgc
     7621 ggacgggaga actgaacagc cgcgtcatgc agcaggcgaa accagtagtg gtcccgctcg
     7681 ggtgacggaa gcgagcagcg cagacggtcc aaaacatcgc ctatgcttcc gcagacctga
     7741 cgccctggat cactccgtcc cgccaggacg gaaggatcct cgcagacgtc gaaataaggg
     7801 cggtcgagcc gaagcgtgtc atgtgcgagc cctctgttgg tcaggcccag gtagatgact
     7861 aggtcggagt gcgaccttat ccgctcacac tccacgctgc cggagctacc aatcatgcca
     7921 aggcagtgct tgtgagaggc gtaacgccac agcgacccca tggtacacag tactggaatc
     7981 ccggcctgcc ctgatgcctg catcaccttg tcggcgcacg gaatgctgcc ggaaccagca
     8041 acaataaccg ggcgccgact gctgcttaac gcgaccgaca gtgcccccag attgataaag
     8101 tcacttccgg cggaataacg acgcgaacga ctgcgcagcg cgggaaagta ctgtctgacc
     8161 ctatccgagc cgacaccgtc gacctcgatc tctacgtcgc cgccgtcgaa cgcaatatcg
     8221 tatatcccgt tcgccgccga cagcagcgaa aataccgagc gggacgacac cgcggtcctg
     8281 tatccaagtc gggcgcaccc accagccgcg agaacggacg cctcagcggt cgctgactga
     8341 acggtacggc tctttccgtc cctctcaacg gcgacggtga cctggcgggg acggaccgag
     8401 aaccctgact ggtcgactgg gctgaaaccg aaccgagcgg aggccgggct catcggccta
     8461 ttctggatag tgtccgtggc gcagcaagtg gcaagaaagg acgtgctgcg aagagccggt
     8521 ccgatgaccc gttcgcagac cgttctgccg ctatcgctgt catacaggag ctgtatgctg
     8581 ccggtggccc ggcaagccgc acagatgaag tcagccaggc agtcaaggac gtccgccagg
     8641 tagaggaagg acttgctgac ctcggtggct gtgcggctcg ccgcgagcaa gtacccgaca
     8701 ccccagcccg caggcaactg cgccccggac ccactcctgg cccgagaatc aggctccctg
     8761 gcatcatggc ggagaagact ttccgaaata agcgggccac ctgccgctcc tgcttcctgg
     8821 cgggttggcg ggaggggagg agaaggatgg gagcatggcg gtagactgcc atcatcctgc
     8881 cacggcctta cgtgccccaa cgccgacggg gagccgacaa atgtgtccct atccacaccg
     8941 acaatgcagg gaagctgctc tcggtcaacc agatcaagcc gggaagcgtc gaagcgagcg
     9001 tcaaccatat gtataccgag gttgtgcgcg aacctctccg tttcgatgtc ctgaaaagag
     9061 ccgggtgggt ctcccggcgc tggcatgcct atcacgaaga cagtcggcaa ccggtgagcg
     9121 tgcgcataga caatggattg caggaggtgt gcagccccag gccccgaggt gccgacagca
     9181 atcgctggcg cgccagtttg caggtagcac gcgagggcgg cacagccagc tgtcttctcg
     9241 ttgaacactt ggtgaaaaga ggtgtcgcat gccgagatca acgagttgca ggtaccgttc
     9301 acggagtcgg cagccattcc gaaggcgtgc cggacaccca tccgggcggc cagacggagg
     9361 agcgcgtcag gcggggtcat gtgttcgtcc tttaatgtag atattgagcc tcagaacacc
     9421 aagcaggaac gtagtcgttg acatcaacga gaatccggca gcctcaaact cgtggacaac
     9481 actcctccag tcagggctca tcctgatctc gcgcactgtg tgtgccagga caggtcggct
     9541 gagaattgta agcagcgact caagcgcgcg caggacccgg accagcagcc aacgcatcag
     9601 cgggctttct ggcagcgata actctaatga gacaagtcgg cccgctgggg ccagcagcct
     9661 cccgctcatg gcaagtacct gctcaccgcc atcccccagg tcgtggtaag ctagtgacag
     9721 caccacacag ttgaacgagc caggcggaag ctcgagggta gccacgtcct gggccagcac
     9781 aaggtcgtaa cagtccctgg agactgcctg agcaagcatc gaccggttga tgtcgacgcc
     9841 gataattgtg ctgccgggat agcggagacg gagcgcccgc ccaaactcac ccgtcccgca
     9901 gcacaggtcc aggaatttgt tggaagttgt gggcagagaa tcgatcgcct ttctgcgtgc
     9961 aactgtaatc ctgccgcagg tcagtatcct gttgatcgag tcatagcggc gggcgacggc
    10021 ggtgaagttg cgctgcggcg tcgtcggccg accggccgtc tctgctgatt ctgacgacac
    10081 tgtcacagtg tctccctgat gagatccttg agaggcatcc agcacccttc gcatgacagg
    10141 gaagggcgtc gactctttgc accagctccc gcctaaattt cttgagcaat atatcaacct
    10201 cctgcgcctg gggacgctgg acctcgcgcc ttggtcttac tgcggccgac gtttcctcga
    10261 cgcctccgtt gttgacgtac tcacgcacgg cctcaaatag atcaaaacct gcgccgtgca
    10321 ggtcaaacgc tgtgacgagc ccgttcgact cgtcgatacc aagcggcttt ccggcgggga
    10381 cggaactggg gagcaccctc aggagatcat cgcgtacctg gaaggccata ccgaatagtt
    10441 ctgccatctt gaggagatcg tcgcgtgctt cgaacccacc gggtgccatc gcgaccgcta
    10501 tatcgcacat tgcaacaaag tacgacgcgg tcttgttgcg gatgacccgc atataggtcg
    10561 ccagggagag ggcctcctgg ctccgaacgg ccgttgtagc gctcctggtg gccgcctcct
    10621 cgagatgctc accttcacac agcctcgtcc ccatgtcagc aatcgtgcgg actatctggg
    10681 tcgcgacgtc gggtgggcac gccgacgaca gcccacggaa catcgaaaag agcatcgcgt
    10741 caccagttat gattgcggta gcggcgccga acttcgtgtg gagtgccgac cttccacgcc
    10801 gcagtggcga tccgtcgatg atgtcgtcat gtatgagtga tgctacatga cccatttcta
    10861 tagcttcagc gacatccagt aggccagagt catccggttg aagtcctaga cctgcggctg
    10921 tgcacacaag caggcgaggc cggactagtt ttcctggtac ttcacaactg tacgccgcac
    10981 gggctcccac tgagctgggc atggtgctga ttgctcggct aatgtgctca acgatttctt
    11041 cctcaagact gctgctcatg tctctccgag gttctggaaa tataccagcc cgccctggag
    11101 tcgagcggta ctatcatggc ggttgcaaga cccagcattg acaacagcca cagattcatg
    11161 tgatacccag cctcagcggg aaacgcccag gacgcgacga agagcgtggt ggcgagcatg
    11221 tcccacttca ttctggcgtt cagggctagt gcgtcaaatg tgcggacgaa ggacgtcgcg
    11281 tgccccacac acagcaggag aacaacccaa tgcatgatta tttttaacgt ggatgccgtg
    11341 gatgcgaaca catatacaga cgctgcgaca taggctcctg cctcgaacag caccgccgtt
    11401 actgcggcgt tcctgtaact ggtgaagatt gttgctgtcg ccttgaggcc ggcccgcttg
    11461 tccgcgtcaa agtctggtag attcttgata aagaactttc cgcacatgac gagggcaaga
    11521 agagctgtgg gccacagcct gagctgggaa ccgccgatag tggcccagcg gttcgacaca
    11581 agagaaccgc cgatatatgg cagcgccgcc actagggcga acgtgcatgc cccggcaagc
    11641 ggttgggact taagccggaa gggcggcata gaatacatgt acatgaggcc aatccctaca
    11701 ggcgcgacgg cgagggtggc gaggctggtc gcagccagaa gaagagccag cacaccagtg
    11761 gaagagacga gcactactgt gacgcgggtg cttccgagcc tccttacggc cagaacccgg
    11821 ttgctgaggt ggtagctgtc ctcagtgacg tcggtggtcg cgttgtggag gttggctact
    11881 gcgggcacaa ggtaactggc aatgagcgcc gacaggagcc gccacgtcca ggagattcct
    11941 gtggtgctga aggctagcag gagagagaag acggacacga agcaggtccg cggcctggag
    12001 agggcgacta gggttaccgc agtcgcacga ccagcaaacc gctttgccat gtataaagtg
    12061 taacactcga gacatcatca ttcatgtgta gcgaaacact actatttgca ttcttgcctc
    12121 cactcccggc gggccgcagg caactcgctg gacctgaccg gtcccgcctg ggtggtcccg
    12181 cagagtgcgg ctgctcagca gaaccaggca gcgcagacgt cggggcgcag ccagtcagga
    12241 gacgcgggtt ggcgacggct gccaccccag tgtgcgggcg gtctccggca aggacctctg
    12301 cgtccttacc gggggtccgg ccgcctacgc cgcagctcct cagagaggcg ggcgtggagc
    12361 tcctcggatg tgacgcccag ggggtctgtg gagatagagc caggcccgaa ccccgccata
    12421 ccctcattgg ctcgcacggt agcggccttg agcccgctga tctgggtcct aggccactgg
    12481 gggtcgacga agtcgatctg gaccgagtcg atgtcctgga tacccatacg gacccgaagg
    12541 gggcggaagc cggccacctg ctcccagggg acacggcgca cgcagaccgc gttcgtgcgg
    12601 ttgtcaatcc cggcgtcgtc cacgcgcagg gcgtgaccac gccccagccg tgctgtcctc
    12661 accgcggtat aggagaagaa cacgatgccg agaaggccga cggcgacgca ggtgagccgt
    12721 gccgagccgc ttcccagcat cgcaaccagt accctcggcg gctcgatggc aaggacgacg
    12781 atgctggcta tgacgagcac gagcgacccc gccactcggg gcaggatgta agaggacttg
    12841 tgcttgatga cgagtggttc catgccccca tcctcacccc aggcacccgg ttcccgcagg
    12901 gcgtgtcgag gtgtaccaaa tcctcacaga tgactggcgc gtcggtactt tatgaggatt
    12961 ccgtacacct gtgaggatcc ggggtcctgt gtggggtggg aggtggggtg ggaggggtgt
    13021 ccgcagcgcg ccctagagca gcaccgtggc gaaggtgtcc acctgggtga atcccaccga
    13081 gcagtacagg gccagggcgg gggcgttgaa gtcgttgacg tagaggctca ccgtgccctg
    13141 cggccccacg tggtcgcggc gcaccgcgtc caccacggcg gccgtcgcga cagcccccac
    13201 cccccggcca cgcaggtccg gccgggtcca caccccggtg agcatggcca cgtggtcccg
    13261 cgcgctgtgc cacacggagc cgacgtccgc cttaaaggcc accatcgcag ctgagccggg
    13321 gcgcgcaggt gccccggtac cgtcgtccag gacgacgtag ctgcgtcccg tggacaccag
    13381 ccagctgacg tggcgcgcgt aggaggagcc tgtagtcgtc gggtcgtagc cgacctcggc
    13441 ggtgaacatg gccaccgagg ccggcatcac caggggctcc tcccgccgcg tggcagcgtg
    13501 aagcccctgg gccacccagc ccagagcggg ccggcgtcgt cgctgacctg ccacgagccc
    13561 ctctcccccg ggcgaggcag gagcgcgcag cagaggctgg ttccagcgct cctcgcgggg
    13621 tactgccccc agcctgcgca gcggctccca caaggccgcc acgtcccggg ccacaccgac
    13681 caccgagccg tgccgaccga ggcggggcct ggtgtgctcg gccagggcca ggagcgtgga
    13741 acgcgcaaca ccaccatgcc cggtaccagg cctgggggcg agcccaaagg gcatagcgac
    13801 acccgtggtc caggccgcgc cgacaggcgc ctgggggtct cccgccacga cgacgtcccc
    13861 ccggccccac ctgctccagc gctgcacctg gtgagccagc ggagtggcct ggaccggctc
    13921 cgtgccacac aggcccagca ggcctccgac cctgtctgca gccacaggca ccaggcccgt
    13981 gccctccggg cctcggcgcc gcaaccgact caccaaggtc ataccgctct caacccgtcc
    14041 tcaacccgct ctcaacactg tccgcatcct tggcctagac cctcctcaga cctcgtgtcc
    14101 tctcagacca cgccctgcta gccggcagcg gagaccatgg gagccgtgcc caccagcgcc
    14161 tcctcgccaa gctcacgggt catgtcctcg gccatgtcct gggcgtagcg cagcagggtc
    14221 tccaccacct gctcctcgcg gacggtctcg accaccttgc cacgcacgaa gatctggccc
    14281 ttgccgtttc ctgaggcgca gcccaggtcg gcctcccgcg cctcacccgg accgttgacc
    14341 acgcagccca tgacggccac ccgcagcggg gcggtgacgc ccttgagccc ctcctcgacc
    14401 tgctcggcca gggaccacac gtctacctgc gcgcgcccgc aggaggggca ggagacgatc
    14461 tcgagctgac gctggcgcag acccatgaac tctaagagct tggtaccgac cttgacctcc
    14521 tccacgggtg gggcagacaa ggacacgcgg atcgtgtcac cgatcccctc cgccagcaag
    14581 gtgccgaagg cggcgcagga cttgatggtg ccctggaagg ccgggccggc ctcggtgacc
    14641 cccaggtgga gcggccactg cccggcctgg gacagcagcc ggtaggcctg gaccatggtg
    14701 accacgtcgt ggtgcttgac ggagatcttg aagtcgtgga agtcgttctc ctcgaacagg
    14761 cctgcctccc aggtggccga ctccaccagc gcctcgggcg tggccttgcc gtacttcttg
    14821 agcaggcgcg ggtccaggga cccgccgttg accccgatac gcaggctgac gccgtggtcg
    14881 gaggctgcct tgcagatctc ggccacccgg tcgtcaaact tgcggatgtt gccggggttg
    14941 acgcgcacgg ccccgcaccc ggcctcgatc gcggcgaaga cgtacttggg gttgaagtgg
    15001 atgtcagcga tgaccggaat gcgcgactgc ctggcgatga ccggcagggc ggcggcgtcc
    15061 ttgtcggtcg ggcaggccac gcgcacgatg tcgcagcccg ctgcggtcag ctcggcgatc
    15121 tgctggaggg tggcaccgat gtcgtgggtc ttggtggtgg tcatcgactg cacggtgaca
    15181 ggggcgtccc cgccaacctc aaggccgccc accctgatct tgcgcgtggg cctgcgcggg
    15241 gccagcacgg gagcctcggc acgcacggag gggataccaa gatcgatcac gtcgctcacg
    15301 ccccaagtat gtcaccggcg atcactacca gccccgcccg gaggtgtggc atggctccct
    15361 gcctcggctc aggtcaccgg cgccacgagg tccgcccaca ccagcaccac cgccatgacg
    15421 acaagcaggg cgacgacgac ctggctgaca ggcagcagac gggcgatgtc caccggccca
    15481 gggtcaggtc gcccgcgcgc agcagcccag cgcctgcgga gcccctccca gcacgccccg
    15541 gccacgtgcc cgccgtcaag gggcagcagc ggcaccaggt tgaaggcgaa cagtgccagg
    15601 ttgaggctgc ccagcaggct cagcatggct gacacccgtg ccgacaagga gacggtgccc
    15661 tcaccgaccc ccgcgctgga gacctgcccc gcgtggcgtc ccagccccac caggccgatg
    15721 accccggtgg tgctgcgctc ctccagcccc aggccggcgg cgaccacgtg gtacaacccc
    15781 accggcaggg tggcgatcgc ccgcagcgtc cccccgacgg cctggccgac catccccggc
    15841 agctctccca ggggcacgcg ccgggtctca gaggcggggc tgagccccac atagggtcgc
    15901 gaacgggtga gggcggtacc ggtggagtcg gtgaccgggc tgccctggtc gtcgcgcacg
    15961 gcacgctcca cctccacggc cgtcacgctg acggtccggc gcgccctgtc acgctcgacg
    16021 accacctccg tcggggtggt ccctgcccgt tcgatctggg cagtcacctg ctgccaggtc
    16081 gagaccggga caccgttcca ggagatgatc cggtcccccg gctccagtcc ggcccgggcc
    16141 gctggggaga ctgggtcctc ctcggtgcag ggcgcaccgg cgtcgatgtc ggaggagacg
    16201 cagggggcga cggaggccag ggaggaggtg ctcgtcggga cccccaccac gccgaggctc
    16261 acggccagca gcacgacacc caggacgagg ttggtgagga tgccgccggc catgaccacc
    16321 agcttgcggg ggacactgag ccggtagaag gcacggtcct cctcaccggg ggcgatctcc
    16381 tccatggagt cggcccgggc ctggcccacc atgcccagct caccgctgct ggtgcgccgg
    16441 tccggacggt cggggttctc cggcggcagc atgccaacga gcttgacgta gcctcccagc
    16501 cacagggcct tgaccccgta ctctgtctcc ccgcgccgca ccgaccacag ccgcgggccg
    16561 aagccgatga agtactcggg aaccttgacc ccgaacttct tggcaggcag catgtgaccc
    16621 agctcgtgga gggccacgga cacggcgatg ccgaccacca tgacggcgat acccaggacg
    16681 taggcaagtg cctggctcat gcgtctcctc ccacgtgccc ggaccgggcc tcgaccatct
    16741 cctgtgcccg cgcacgggcc caggactcca cctccaacag ctcgtcaaga ccaggactgc
    16801 tgaggccctc gtgctctgcc aggaccgcgg cgtcgatgct ggtgacgtcc agccactcca
    16861 gcccgccgac caggaaggcc tccaccacct gctcgttggc ggcgttgagg accgcagggt
    16921 gggtgtcgga ggccgacacg gcctggcggg ccaggtccac cgccgggaag gtctgggagt
    16981 ccaggggctc gaaggtccag gccaccgggt cctcccacct ggtgggctcg accagtcctg
    17041 acaggtcctc ccggtccggc caggtcaggc ccagggcgat gggcaggcgc atgtcgggcg
    17101 gggaggcctg ggcgatggtg gcgccgtcaa ggagctcgac catggagtgg accaccgact
    17161 gcgggtggac gacgacgtcg atgtcgccgg ggtccacgtc gaacaggagg tgggcctcaa
    17221 tgagctccag gcccttgttg accatggtgg aggagttgac ggtgaccaca ggtcccatcg
    17281 accaggtggg gtggtccagg gcctgggccg cggtcacgcc gaccagctcc tcgcggcgcc
    17341 ttccccggaa gggccccccg gaggcggtca gcaccagtct gcgcacctcg ctgcgaccgg
    17401 tgaccagggg gctggtcagg cccttctcgt ggcgggcgga cagcaggcac tgggcgatgg
    17461 cggagtgctc ggagtccacc ggcaccacct gcccaggccg ccgcagggcg tcacgcacca
    17521 gcgcccctcc cacaaccagt gactccttgt tggccagggc cagacgggca ccgctgcgca
    17581 gggccgccag cgtgggcagg agtcccacgg agccggtgac ggcgttgagc accgtgtcgt
    17641 tgcgcgcagg ggcagcagcc tccaccacgt ccaccgtggc ctgcgggccg gtgaggaccg
    17701 tggtcacagg gttgggcacc gcgctgcagg cagccgcccg ggacagtgcc tggcgcaggt
    17761 gctcccggac ggcgtcctgc tcccccccct ggcaggcgac ggccaggacc ggtacctgcc
    17821 aggccacggc ctgctcggca agaaggtcca ggcgggagcc acctgcggcc agcgccgcca
    17881 cctgcggcgc gcggtcaccc agcctggtga ggacctgaag ggcctgggtc ccgatagagc
    17941 ccgtggagcc cagcaggacg agacgagaag gttcagtcat gaggatctgc ccgagggtgt
    18001 ctgcccggag gctgtgtccg ggacctgtgg atgatgccgt gcacgtacgg cgacggctac
    18061 cctacacggg tgtggccggg cgcccctcac caggccagcc ggtgttaccg cccgctcgac
    18121 caacagctac tccaccgaca gcaggacctc caccacacgg gcgagatagc ggtccacact
    18181 ggcctcgtcg taggccttgg agccgcgcgc cgcccggaag accgcgcccc tgacctcaga
    18241 ggaggccagg gcattgtcag agtcgaagta gcccgcgata cggtccatga gcgcgtccac
    18301 gtcctccttg gcgtagccct ggccaccagc aggcaggaag cgctccccct cgggccggag
    18361 cagcctgggg tagagggtgg tggccagctg ggtgacctgg tccatccagg cacggcggcc
    18421 gtgctgggcc acgaaatcgg cacggcgtcg ctgcaggaag gccgcctcga gacggtcaag
    18481 ggcggcgtcg accgcagcgg gctggtaccc cccgggaacg atgtcaaagg cgaccgtgcg
    18541 caccccctcg gagtccatct cgtcgagctc gccggcgtcg tagatctcgc gtgcggtctc
    18601 aaagtagcgg tccacctgct cagggcggta gcccggtcgc aggggcatgg acttgggaaa
    18661 catgttgctc attgcgtcct tgccctctcc tggttgagta cctccgtggc gagctggccg
    18721 caggcaccgt cgatgtcact gccccgagtg tctcgcaccg tagtggtaat tccagcacac
    18781 cgcagcgtgt cgacaaagga ctgctgggcc tggggcgtag agcaggtcca gatcgaccct
    18841 ggggtcgggt tgagggggat ggggttgacg tgggcccagc ccctgcccct gcggttgagc
    18901 tcctcggcca ggagctgcgc ccgccaggcg tggtcgttca tgtccttgat gagcgcgtac
    18961 tcaatggaca cgcgccgccc ggaggcctgg aagtagtcgt aggcggcgtc caggaggtca
    19021 gcgaccttcc accgggagtt gaccgggacc agctcgtcgc gcagggcgtc gtcaggagcg
    19081 tggagggaca cagccagcgt gaccgggagc ccttctccgg ccaggcgacg gatgaggggg
    19141 accaccccca ccgtggagac cgtgatgccc cgcgccgaca tcccgaagcc ctccggcgcg
    19201 gggtcgacga ggcggtgcag ggccgccacg acgttcctgt agttgatcat cggctcgccc
    19261 atccccatga agaccacgtt ggacaggcgc gcgggcccgc ccgcgaggtc ccctctggca
    19321 gaggccagcg cggcgtgacg aacctgctcg acgatctctg cggtggacag gttgcgggtc
    19381 agccccatct ggccggtggc gcagaagggg caggccatcc cgcagccagc ctgggaggac
    19441 acgcacaggg tggtgcggtc ctcgtagcgc atgagcacgg actccacgcg cacgccgtcg
    19501 tggagctccc acaggtgctt gacggtgcgc ccggcgtcgg cacgcagggc acggacctcg
    19561 cagatgaggg gcggcagcag ctcggcgcac agctggtccc gctgggacgc tggcaggtcg
    19621 gtcatacgcg cggggtcgcg tgtgaggcgg atgaagtagt gccgtgagac ctggtcggcc
    19681 ctgaagcctg gcaggcccgc gtcctggcag gcagccttgc ggcccgccag gtccaggtcc
    19741 gccaggtgcc ggggtgccct gccacggggc gctgggaccg ccagggacag ccgggggcgg
    19801 gcgtcaggac tgctcgcgcc ctcgggaggc tggtcagtcg ggaggacctg gacctcgccg
    19861 ggagccgggc cagcagggct ggcagaaccg ggcgcccggt cagcgggacc gtgccgggcc
    19921 gcgcgcccgg ctggggcacc aggacggtgg ctggcaggac tcactgtcca cacgctcctt
    19981 tccgggcggc ctcaaagacc gcctaccaca ccacgagggt gaagacgtag accacgggcg
    20041 cggccacgag aaccgagtcc agacggtcca tcatcccccc gtggccgggc aggagggtgc
    20101 ccatgtcctt caggcccagg tcacgcttga gcagcgactc tcctaggtcc cccagcgtcg
    20161 agaccaccac gacgcacgcc cccaggaccg cccccgccca caccggtccg tccagcaggt
    20221 acaggagccc cgcccccgcc gcgaccgtgg ccagcaggga gcccacgaac ccctcccagg
    20281 acttcttggg agacaccgac ggagccatgg ggtggcgccc aagcagcacc ccgaccagcc
    20341 agccaccggt gtcactggcg gcaggcaggg cgatcatcat catcacccgc tgcgccccct
    20401 cctcctgcgc cgccaccagg accgcgaagg aggccaggaa gggcaggtag gtggcggcga
    20461 acaccgatgc cgccgcagag cgggagcgcg agcggcgcac catgtcgtgg aggtcgctac
    20521 ggggcacggc gttgccggac tcctccagcg gcgtgccggt gtccgcctcc gcctggtcca
    20581 tgaagcacca caggacgcag gcccccgcag tagccacgta ggagccgaag gccgcctcgg
    20641 cacccacctg ccacgcgcac acggcagtac ccagggtccc cagccacaga ggagccagtg
    20701 gcaggcggat ccgcttacga gccagtgcac ccgccatctc ccactgcgcc ccgcacacgg
    20761 ccaccacggc caggaggacg aacagctccc tgtagaacag caacgacccc gcgactagca
    20821 ggacgaggac gactgccacg cccacagcgg caggaaggtt gcgcccggca cgccctgtcg
    20881 cgggaagggg ggcgtggtca cgcgtggggg gcggggtcag cagggtgctc acgaggccca
    20941 cggctcacgt cttgaggtca tcagcagtcc tccggtcacg ataggtcacc tcccgtccag
    21001 gcccggggct cctcggacgc cgtagcggcc tcaacgtcgc gccgctaacg gaccccagg
//
