LOCUS       NZ_CP025227            21155 bp    DNA     circular CON 14-APR-2024
DEFINITION  Actinomyces wuliandei strain 299 chromosome, complete genome.
ACCESSION   NZ_CP025227
VERSION     NZ_CP025227.1
KEYWORDS    RefSeq.
SOURCE      Actinomyces wuliandei
  ORGANISM  Actinomyces wuliandei
            Bacteria; Actinomycetota; Actinomycetes; Actinomycetales;
            Actinomycetaceae; Actinomyces.
REFERENCE   1  (bases 1 to 3107761)
  AUTHORS   Zhu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2017) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No.155 ChangBai Road, Changping District, beijing
            102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. HGAP 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004010955.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 19:14:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,606
            CDSs (total)                      :: 2,544
            Genes (coding)                    :: 2,496
            CDSs (with protein)               :: 2,496
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 18 of 48
            Pseudo Genes (incomplete)         :: 37 of 48
            Pseudo Genes (internal stop)      :: 3 of 48
            Pseudo Genes (multiple problems)  :: 9 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:15:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1261521
            Orig. end                         :: 1282676
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21155
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="7"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21155
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21155
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11155](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11155)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            complement(1117..1740)
                     /locus_tag="CWS50_RS05235"
     CDS             complement(1117..1740)
                     /EC_number="1.15.1.1"
                     /GO_function="GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549597.1"
                     /locus_tag="CWS50_RS05235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="superoxide dismutase"
                     /protein_id="WP_127841935.1"
                     /transl_table=11
                     /translation="MTVYTLPELPYDYAALEPHISGRIMELHHDRHHAAYVAGANAALE
                     ALAAAREAGDLGAVNLWEKNLAFNLGGHVNHSVFWQNLSPHGGGEPEGELAAAINDSFG
                     SFAAFQAHFTATALGIQGSGWAVLAYDSVSGGLVVFQLFDQQGNIPVGTIPLFMVDMWE
                     HAFYLDYLNVKADYVKAIWSIASWEDVSQRLSDAVARSQGLIVR"
     misc_feature    complement(1857..1871)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.19"
                     /tool="antismash"
     gene            2098..2538
                     /locus_tag="CWS50_RS05240"
     CDS             2098..2538
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565060.1"
                     /locus_tag="CWS50_RS05240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_127841936.1"
                     /transl_table=11
                     /translation="MTDQADARLELLVFSDDATVRREVIEGVGRRPARGLPLVTWKEAA
                     TAEGVRLALRDREEEGLAPFDALVLDAEARKLGGMGLAHELFIESDQRPPVVLLTARPQ
                     DSWLAAWAKAEVVVPRPLDPLSLQEGVARALTARRGTVVPAG"
     gene            complement(2557..4368)
                     /locus_tag="CWS50_RS05245"
     CDS             complement(2557..4368)
                     /GO_function="GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549599.1"
                     /locus_tag="CWS50_RS05245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="WP_257493288.1"
                     /transl_table=11
                     /translation="MSTAVPEEPDHGAGPPWRGRGEQASLTDMGTPLREVTFVVVDLET
                     TGAPPGAHALTEIGALKVRGGKVLSRFSTLVNPGAAVPPQITALTGITNAMLVDAPGVG
                     PCLEGFLSWAGLGPDDAGRTCPDAPRSVGTGRSTGTVLVAHNARFDIGHLRGAARALGI
                     GWTEPRVLDTLALARKAWPRGEVANHRLSTLAALVGSPTRPTHRALDDASATADVLHAA
                     LEVLAPLGVTHLEDLATATDPVPARRRARSRLAKGLPTTPGVYQLCSATGEVLYVGSTV
                     NLHRRVRSYFTAAERRPRVTRVLDTTAEVRHVPTPTLLEARARELRLIAELDPVANRRS
                     RAPRRQPWLHLVSGPEPHLALTTVLPLHEVGYAVGPFWSRRSAQEALRAAESVLRLRHW
                     DDRAQGNGAGRSTASEPLTGQASDRAVRAASFLSGQADLVATEVLERVRHLAQAQRYEE
                     AGTWTRRLRSLLQAALRAERARPLLTCPHLVAARPREHGGWELVAVRWGMLAGSLVTPR
                     GADPRPGVAALRSSARVVSQPTLVGQQASVEETLLLADWVLEAGARLVEVDGDPQALSW
                     PAASAARYSKVLHADLHPADDRPRHRR"
     misc_feature    4375..4392
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.91"
                     /tool="antismash"
     gene            4466..5395
                     /locus_tag="CWS50_RS05250"
     CDS             4466..5395
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565056.1"
                     /locus_tag="CWS50_RS05250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="WP_243118473.1"
                     /transl_table=11
                     /translation="MGYGPIKVAVGPALEMLYQPWIRGEENIPAEGAAILASNHLAVID
                     SFFLPLLVDREVAFIGKADYFTGKGLRGWAVRRFMTTVGTIPVDRSGGKASQAALQAGI
                     DRLRSGELFGIYPEGTRSPDGRLYRGKTGVARVALATGAPVVPVAMIGSNLAQPIGQAI
                     PSTRHRVGIVIGEPLDFSRYQGLENDRFVLRSITDEIMYALMALSGQEYVDLYAADVKN
                     AMDAEKKTAEEVVTQMLAAQAQARPSAAPVAAPGGRPAPDVEVPLPPQEDQGETEESQD
                     EVGRSGQQDASSAPGGGSSSAEEPPAPE"
     gene            5527..6756
                     /locus_tag="CWS50_RS05255"
     CDS             5527..6756
                     /EC_number="2.7.1.90"
                     /GO_process="GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565052.1"
                     /locus_tag="CWS50_RS05255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="WP_127841937.1"
                     /transl_table=11
                     /translation="MSIRRVALLTAGGFAPCLSAAVGDLVERYTQVAPEVEIIAYQYGY
                     HGLLTGSYVVVDEEGRRNAGILREFGGSPIGNSRVKLTNAKNLVERGLVAEGVDPLAFA
                     AEQLRKDGVDVLHTIGGDDTNTTAADLAAYLHDNDYELTVVGLPKTIDNDVVPVRQSLG
                     AWTAAEQGAEFAFNIIGEHRSNPRMLIVHECMGRNCGYLTAETARRYRELLETKQWAPS
                     LGLSKERWDVHAVFVPEARLDIATEAERLKAIMDEQGNVNIFLSEGAGVPEIIAEMEAA
                     GQEVQRDPFGHVKLDTINPGQWFARQFADLIGAEKVMVQKSGYYSRAAAANAEDLALIK
                     QMCDHAVDCALRGVSGVIGQDEENNDELGAIAFPRIAGAKPFDITQSWFTELMTDLGQS
                     VEPAEAAAQH"
     gene            6906..8264
                     /locus_tag="CWS50_RS05260"
     CDS             6906..8264
                     /EC_number="2.5.1.54"
                     /GO_function="GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565051.1"
                     /locus_tag="CWS50_RS05260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="WP_243118523.1"
                     /transl_table=11
                     /translation="MNDLDAPRSHGPAWRDRPAFQQPVYPDAAALDAVGADLRSRPPLV
                     FAGEVDALRCQMAAAADGRAFVLMGGDCAESFADTSATSIRLKVQTILQMAAVLTYAAS
                     TPVVKIGRMAGQYAKPRSADTETREGVTLPSYRGDSVNDHAFTPEARTPDPARMLEAYL
                     RAGTTLNLVRAFTMGGYADLREVHLWNRGFTANPAYKRFESFAEEIDRAMRFMEAAGVD
                     FEALRQVDFYSAHEALLLEYEDAMIRQDSRTGRLYGTSAHMLWVGERTRGPRDAHVALL
                     EGVRNPVGVKLGPTTTPEEVSSLMDHLNPEGETGRLSLITRMGAERIREVLPGLVEAVR
                     RDGRPVTWITDPMHGNTITSDNGYKTRRFDTIMDEVRGFFEVHRDLGTVPGGVHVELTG
                     DDVTECLGGGEHIDEQRLAERYETLVDPRLNHQQSLEMAFEVAEMLQDRSRTS"
     gene            complement(8346..9968)
                     /gene="pknB"
                     /locus_tag="CWS50_RS05265"
     CDS             complement(8346..9968)
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="pknB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733787.1"
                     /locus_tag="CWS50_RS05265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="WP_127841938.1"
                     /sec_met_domain="Pkinase (E-value: 1.2e-50, bitscore:
                     169.5, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKDPLIGRLVDSRYEIVAQVARGGMATVYRAQDRRLDRDVALKL
                     MHAHLADSPEFVARFRREARAAARLSNPGVVAVYDQGSLDGVAYLVMEYVEGPSLRDLI
                     AAGPLSVGEALGLVAQVLRPLGAAHRAGLVHRDIKPENVLLPADGAVAKVADFGLARAV
                     TEVAQTTTGNVLGTVAYLAPELITSGASTARADVFSVGVVLYELLTGSLPFHADSPIQV
                     AFRNVHEDVPLPSTTVPQMSANVDELVRAMTRRDPLQRPGDADAALTLLRHVAGELTGS
                     DLSVRPGGGTGSERTHEVMAANARAARSAMAEAPEPDQERDTPSAGMRTVSLPIGSIGP
                     EPAETGTRQLPRRSLLTGDRTQQTVPVPSEATSQDSARRRYGRRALLTGAGVLVLAGGG
                     YATWYRTVGPGRRITVPDIVGMTQQEAHSAVEGQGLVWASPVRAYSDTVAAGLVISSTP
                     GAGERVTRDRAVTAVVSRGRQRVDVPDVVGMSEEEATDTLEEAGLGVEVSRVLGGLLGK
                     VRSIEPPAGTSVTVGSTVTVTVL"
     gene            complement(10001..11155)
                     /locus_tag="CWS50_RS05270"
     CDS             complement(10001..11155)
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009747443.1"
                     /locus_tag="CWS50_RS05270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_127841939.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.3e-39, bitscore:
                     133.2, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDLPEPLTVVREAVESRLGQLLADCRHRWDDLGDAGGDMLDAAS
                     QALGGGKRMRAILGAVGTGLLAPHTLRPQVLTSPQSVHLATALELYQASALVHDDVMDA
                     APRRRGQPASHRVFARTHAANHWLGDAEDFGTSAAILLGDLLLSLAGEEMGRATAVLPL
                     ADTVAGEHARAAFDAMTTEVALGQFLDVRSQALPLPRQEDPLRASARMHASSLSVVRHK
                     SARYSVQHPLVIGALLAGARPDSALLDHLRDFGEEIGIAFQLRDDDLGVYGDPALTGKP
                     AGDDLREGKRTVMLALAWGRTDDAGRRVLEQVLANRAAGEAEIARAAQVIEDCGARAAH
                     EEQIQAHRQAGYQALERLRAQEGLSPACLEDLETLALVLTERST"
     gene            11375..13036
                     /locus_tag="CWS50_RS13455"
     CDS             11375..13036
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009747452.1"
                     /locus_tag="CWS50_RS13455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_127841940.1"
                     /transl_table=11
                     /translation="MASTLTRTRVALTDDVNETADDFDLDDEGEEDLDEVPDKDSEDSD
                     SPDDSDDDPADDSVDPGKGSEDPEGRAVTGADDQEEPRPELRDYYLDVTLETDGDGSKR
                     NPFNTPASLKGVRLAPGATLFIRSRTVVEDLEIAGHGTIEEPITLAPYGHGPVPRFVVD
                     GSEPMVAREFLALNGYIFKGWVIKGIKMQPSIAALTGELERMRREEAEKAAAKKAGRRS
                     RSGDKDGSFTVSDSDEADEPAQRVVTAGATADPVKDYLKQIGKVALLNAEQEVELAKRI
                     EAGLFAEYRLSEEGNDLPARLRRELHWVAQDGQRAKNHLLEANLRLVVSLAKRYTGRGM
                     LFLDLIQEGNLGLIRAVEKFDYTKGFKFSTYATWWIRQAITRAMADQARTIRIPVHMVE
                     VINKLARVQRQMLQDLGREPTPEELAVELDMTPEKVVEVQKYGREPISLHTPLGEDGDS
                     EFGDLIEDSEAVVPADAVSFTLLQEQLHAVLDTLSEREAGVVSMRFGLTDGQPKTLDEI
                     GKVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD"
     misc_feature    complement(11423..11437)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    13390..13405
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            13404..13853
                     /locus_tag="CWS50_RS05280"
     CDS             13404..13853
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF020461.4"
                     /locus_tag="CWS50_RS05280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GAD-like domain-containing protein"
                     /protein_id="WP_164860084.1"
                     /transl_table=11
                     /translation="MRMSEQEWHEAWLRATPEWVDANMDDLVKEGYAPMGVGAPVTAEH
                     VGYFGGVLPASVLHLWERFGFDGFGAGRWWFTDPYRWAPVVEAWLGGTAIPFPHQRWWC
                     LARSATGTMRLWGGGLRPRSDPHPRPGVGASRRSQRLQDVGRGDA"
     gene            13846..14247
                     /locus_tag="CWS50_RS05285"
     CDS             13846..14247
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CWS50_RS05285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_127841942.1"
                     /transl_table=11
                     /translation="MRERMGATTLDSPRKDLGDDDTGVLVADWAIDNLGVLGADQVYGL
                     TPAYCFTGRVSTSQVGIEDAVAHLTFLAQAQEHTLVEDFSAAAAQVAATIATGAQDNGS
                     GSAGGPGDDSGEGAGGPHGPEARGPQEGS"
     gene            14247..15803
                     /locus_tag="CWS50_RS13790"
     CDS             14247..15803
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026942.4"
                     /locus_tag="CWS50_RS13790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polymorphic toxin type 15 domain-containing
                     protein"
                     /protein_id="WP_127841943.1"
                     /transl_table=11
                     /translation="MAGRWRDRPRWLRDGRPPTPAAGPTGRSGGGAAPDHSGGGGQVPG
                     CGGGGGAGTALVASGMFTPGRAGGQSVLVWEGAVAAPASPLAWHSATPVEVFFHTGHHD
                     STQVLVAEFTRQLDRQLSALSLLSAQRLLEGIRAYRAHGRQPTSKTTRNRRKKFMEEVV
                     GDLVHVHRLSWEDAQAATREVDRALVVLHESDQVTYGPSQPATTTEGWPSLGHGVVSSS
                     IGGQGRPVATVLEQATLAVPADQRPRVRLLVRAVLTGSPDLAQDLRHGQVVLEPRTQAQ
                     VNATSPRGPPWTPSHLAATIATGHTPWHPPPARAQEPDQAQEPVLSATTQPGAGQPHQP
                     DRPGPATTQPSPEPPRPGPTRTGATTPGPPHTGTTTPGPPQAGATTAGPGQPPRPLTRE
                     EVLANIASHVAQAARQGDQPHTPRQARAAREAPAGAARAARLREASFPHPPTAAVRTPP
                     AQPQAGTTRTGPAPGQPSRPLTREEVLARITAQAAQQPTQPRTPRQTPTRNRRRNHGLG
                     R"
     gene            complement(16067..16336)
                     /locus_tag="CWS50_RS05295"
     CDS             complement(16067..16336)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009233859.1"
                     /locus_tag="CWS50_RS05295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_127841944.1"
                     /transl_table=11
                     /translation="MKATSTTASTATEATATAAPAATTSVDQTRRPLTTADRCDACSAQ
                     AYVRVVLASGELLFCAHHGRAHAEALQASALFVQDESDRLLSED"
     misc_feature    16566..16581
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: medium, score: 23.95"
                     /tool="antismash"
     misc_feature    complement(16567..16580)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.37"
                     /tool="antismash"
     gene            16701..18989
                     /locus_tag="CWS50_RS05300"
     CDS             16701..18989
                     /EC_number="5.6.2.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565037.1"
                     /locus_tag="CWS50_RS05300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="WP_243118524.1"
                     /transl_table=11
                     /translation="MEGLEAVRKRPGMYIGSTDQRGLMHCVWEILDNAVDEALEGHGDD
                     ITLAVHGDGSIEVRDNGRGVPVDVEPSSGLTGVELVYTKLHAGGKFGGGSYGAAGGLHG
                     VGASVVNALSARMDVEVDRGGRTYAMSFRRGEPGVFDDSAGRGPQSPFTGFTQDSQLRV
                     VGKVRAGVTGTRVRYWADPQIFPRPSEFDAGALRARLRQTSFLVPGLTLRLEDHRPQAE
                     VLAASTTPAADAGSTDDAVHEASLRVAARVAAPVTTGTAATAAGTGRGTRAGAGDLFSA
                     SGQDSSGDVNGSAPGSGAGGAEVFYARGGTADFVDFLASDGSVTDTFRLTGQGTYTETV
                     QQLDAGGHLRPVEVERTCLVDVALRWGVGYDTTERSFVNIIATPMGGTHLSGFEQALTR
                     VLRKRIEADSRALKLTSRDGRVEKDDILAGLTAVVTVRVPEPQFEGQTKEVLGTAPVRQ
                     IVAKVVDTELTALLTSSKRDLKAQARSLEEKIVGEMRARVSARLHKEITRRKTALETSS
                     LPAKLADCRSDDVAASELFIVEGDSALGTAKNARNSEFQALLPIRGKILNVQKASQADM
                     LRNAECSAIIQVVGAGSGRTFDIGASRYGKVILMTDADVDGAHIRTLLLTLFFRYMRPM
                     IEAGRVFAAVPPLHRIEVAGRGRRKKEVIYTYSDEELVETLRRLERSGRSFKEPQRYKG
                     LGEMDAHQLAETTMEPEHRTLRRITLADEAEVVRAESVFELLMGSSVEPRRDFIVQGAR
                     DVDRERIDA"
     gene            19228..20292
                     /locus_tag="CWS50_RS05305"
     CDS             19228..20292
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009233856.1"
                     /locus_tag="CWS50_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_127841945.1"
                     /transl_table=11
                     /translation="MAPVYGSPRGGGWSPWTMLRPGPRSSLSRHLSWMPLSPEDNAELA
                     ALISRAETVDDPPYRTTAQETAEYFIDPTYSGVAGRDEEGVMRAFGLVRLRPAGEIYVS
                     MTGVVDPSRRGQGIGRSLLHWQAERARHLVGAERAVSLADSAASRARRQPAHVVTTVLE
                     GDERMQEHLRHLGFEPRRWYRELRRSLSLDIPVVELDGFLRVEPWTPEIDDAVRRAHNQ
                     AIGEGWGSRSLTPEEWAQGSAYFAPRWSFVATDRSGDRARVAGYLRSSRYEQDWEALGW
                     REGYTDMLGVLRDYRSRRVGSALLCAAMQAYAADGMEYAAAGVDTDNPSGAVDLYESLG
                     YEPTRGTVLYVLDV"
ORIGIN
        1 ggggagccgg ctgactgctc cgccctgacg atcgactccg actccccggc cctgcccacc
       61 gtggagggcg aggtaggcac gcttcccacg gtgacgtgga gcggggaggc ggctccggag
      121 aacctcacgg tggcgactct ggaggagggc gacggggccg aggtcactga gacggacctg
      181 gtgacggtcg gctacgcagg gtggcagtgg gactccgacg ccacctttga ctcctcctac
      241 gggaccggga cgcaggacgc ccaggacacc caggactctg cctccggcgg cactgaggac
      301 ggcggggagg acactgcccc tggcgaggct gctggtcagg agaccgcaga ggcctcggag
      361 gttccggggc aggggcagcc cttggtcctg tccctccagc aggtgatcgt cgggtggcgc
      421 tgcggcctgg cgggccacca cgtgggtgac cgcgtcctga tgtcggtccc cgcagacctt
      481 gcctacggcg aggaggaggc gtcctccggc ggaggcccca cgggcccgct cgtgttcgtg
      541 gtggaggtcg tggacacgct ggaccctcag tccgtcgtgg gctcgaccga ggacgccgtc
      601 atggaaggtg aggacgagct ggcccagcgg ggcgtcgccg tcgagggtga cctgggttcc
      661 ccggtgaccg tgtcggtcag tccggacgcg cctgagccga cggagccgga atacctcgtc
      721 ctggccaggg gagccgggca gcctgtggcc gggacctcga cgatcgtcgt caatattgct
      781 gcggccgcct ggtccggtgg ggaggacacg gggagctcca cctgggagga cggggcaccg
      841 aggcctgtct cgatcgctac ctccgacctg ggcgggctgg tcgggattcc ccagggctcc
      901 cgggttgtgg tcctcaggcc aggggacgag tctctggggc gggaggcggt cgccttcgtc
      961 atggacattg aggcggtcct ctgaccagcc tgcggggtgc ggccgcaggg acaggtggcg
     1021 gtgcgggctg actgctgcac gaagtagcac aaaaaagcac gtggtccccg ggctggctgc
     1081 ccggggacca ctgtcacagg tgttacaggc atgctgtcag cgcacgatga ggccctggga
     1141 cctggcgacc gcgtcagaca ggcgctgcga gacgtcctcc cagctggcga tgctccagat
     1201 ggccttgacg tagtcggcct tgacgttgag gtagtccagg tagaaggcgt gctcccacat
     1261 gtcgaccatg aacaggggga tggtgccgac ggggatgttg ccctgctgat cgaagagctg
     1321 gaagactacc aggccgccgg agacggagtc gtaggccagg actgcccagc cgctgccctg
     1381 gatgcccagc gcggtcgcgg tgaagtgggc ctggaaggcg gcaaaggagc cgaaggagtc
     1441 gttgatggct gctgccagct cgccctcggg ctcgcctccg ccgtgggggg agaggttctg
     1501 ccagaacacc gagtggttga cgtggccgcc gaggttgaag gccaggttct tctcccacag
     1561 gttgactgcg cccaggtcac cggcctcccg ggcagcagcc agggcctcca gggcagcgtt
     1621 ggcgccagcg acataggcgg cgtggtgcct gtcgtggtgg agctccatga tcctgccgga
     1681 gatgtggggc tccagggcgg cgtagtcgta ggggagctca gggagggtgt agacggtcat
     1741 cgtggagtgc ctttcgtggg acggtgcggc ttacgggcct gccagggtcc gcctcaggcc
     1801 cccgagtccg cgacagggct ggaggcggct ggtgcgcagg cccgcagcag gggctctgga
     1861 ccggatcggc ctggttctag gactgcctcg gaacagcctt aggactgagg tcccggacag
     1921 tggccatggt aggcgtccgt gccgtgggcg gacaggagga cccagtagga ttacactcag
     1981 cgcggaaagt caggtcgctg gtcgtgccgc tgatcaacct gttgatcact gaccagggtg
     2041 acttgactga ccctggtgcc aggcagccgc agagaagcaa ctgagaggga ggacctcatg
     2101 actgaccagg cagacgcccg cctcgagctc ctcgtgttct ccgacgacgc cacggtgcgc
     2161 cgcgaggtta ttgaaggtgt cgggcgccgc cccgcccggg ggctgcccct ggtgacctgg
     2221 aaggaggccg ccaccgccga gggggtcagg ctggcgctcc gtgaccggga ggaggagggg
     2281 ctcgcaccct ttgacgccct ggtccttgac gcggaggcca ggaagctggg cggcatgggg
     2341 ctggctcacg agctgttcat cgagagcgac cagcgtcccc cggtggtgct gctgacggcc
     2401 aggccgcagg acagctggct ggctgcctgg gccaaggccg aggtcgtggt cccccgcccc
     2461 ctggacccgc tgagcctgca ggagggggtg gccagggcgc tgacggcgcg ccgtggcacc
     2521 gtggttcctg ccgggtaggg tcttgtcggc ccgctactac ctgcggtggc ggggcctgtc
     2581 gtcggcgggg tggaggtcgg cgtggaggac cttgctgtag cgggcggcgg aggcggctgg
     2641 ccaggacagg gcctgcggat ccccgtcaac ctcgaccagt cgtgccccgg cctccagcac
     2701 ccagtcggcc agcagcaggg tctcctccac gctggcctgc tgccccacca gggtgggctg
     2761 gctgacgacc ctggcgctgc tgcgcagcgc ggccacgccc ggacgagggt cagcgccccg
     2821 gggcgtgacc agggagcccg cgagcatgcc ccaccgcacc gctaccagct cccagccgcc
     2881 gtgctcgcgg ggccgggcgg caacgaggtg ggggcaggtc agcagcgggc gggcacgttc
     2941 agcccgcagc gcagcctgca gcaggctgcg caggcggcgt gtccaggttc ccgcctcctc
     3001 gtaccgctga gcctgtgcca ggtgcctcac gcgctccagc acctcggtgg cgaccaggtc
     3061 ggcctggccg gacaggaagg aggcggccct gacggctctg tccgatgcct ggcccgtcag
     3121 gggctcactc gccgtggacc gccctgcgcc gtttccctgg gcacggtcgt cccagtgtcg
     3181 cagccgcagc accgactctg cggcccgcag ggcctcctgc gcgcttctgc gtgaccagaa
     3241 ggggccgacg gcgtagccga cctcgtggag gggcagcacg gttgtcagcg ccaggtgggg
     3301 ctcagggcct gacaccaggt gcagccaggg ctgcctgcgt ggtgctcgtg agcgacggtt
     3361 ggccacaggg tcgagctcgg cgatgagcct cagctcacgt gcccgcgcct ccaggagagt
     3421 cggtgtgggg acgtgacgca cctcagcggt cgtgtccagg acccgggtga cccgggggcg
     3481 cctctctgcc gctgtgaagt aggagcggac ccgccggtgc aggttgacgg tactgcccac
     3541 gtagagcacc tctccggtgg cagagcagag ctggtagacc ccgggggtgg tgggcaggcc
     3601 cttggcgagc cgcgacctgg cccgtcggcg ggcgggcacc gggtcggtag cggtggcgag
     3661 gtcctccagg tgcgtcaccc cgaggggagc caggacctcc agggcggcgt ggaggacgtc
     3721 agcagtagcg ctcgcgtcgt ccaacgccct gtgggtgggg cgggtcgggg agccgacgag
     3781 ggcggccagg gtggacagcc tgtggttggc aacctcgccg cgcggccacg ccttgcgtgc
     3841 cagggcaagg gtgtccagga cacggggctc ggtccacccg atacccagcg ccctggcggc
     3901 accgcgtagg tggccgatgt cgaagcgggc gttgtgggcg accagtactg tgcctgtgct
     3961 gcgtcccgtg ccgacgctgc ggggggcgtc gggacaggtg cggcctgcgt cgtcggggcc
     4021 cagcccggcc cacgacagga agccctccag gcagggaccc acccctgggg cgtcgacaag
     4081 catggcgttg gtgatgccgg tgagcgcggt gatctgcggc ggcacggcgg ctccggggtt
     4141 gaccagcgtg gagaagcggg acagcacctt gccgccgcgc accttgaggg cgccgatctc
     4201 cgtcagggcg tgcgcgcccg ggggcgcgcc ggtagtctca aggtcgacga cgacgaaggt
     4261 cacctcccgc aggggtgtgc ccatgtccgt gaggcttgcc tgctcaccgc gcccccgcca
     4321 gggcgggcct gccccgtggt caggctcttc ggggacggct gtgctcacgc ggcgaggtta
     4381 gacttccgtt acgatcttcg ccatccgttg gcatcgtccc gacgtccacc acgggtacgc
     4441 ccgtcagcag atctcaggag gccccgtggg atacggaccc atcaaggttg cagtcggccc
     4501 tgccctggag atgctctacc agccgtggat ccggggggag gagaacatcc ctgccgaggg
     4561 tgccgccatc ctggcctcca accacctggc ggtcatcgac tccttcttcc tgccgctgct
     4621 ggtggaccgt gaggtcgcct tcatcggcaa ggctgactac ttcaccggga aagggctcag
     4681 gggctgggcc gtgaggcgct tcatgacgac ggtggggacg atcccggtgg accgcagcgg
     4741 tggcaaggcc tcacaggctg cgctccaggc gggcatcgac cggctgcgct ccggggagct
     4801 gttcggcatc taccccgagg gcacgcgcag ccctgacggt cggctctacc ggggcaagac
     4861 gggggtggcg cgcgtcgccc tggccaccgg tgcgccggtg gtgcctgtgg ctatgatcgg
     4921 ctccaacctg gcccagccca tcggccaggc catcccctcc acccgccacc gggtgggcat
     4981 cgtcatcggc gagcccctgg acttctcccg ctaccagggc ctggagaacg accggttcgt
     5041 cctgcgctcg atcactgacg agatcatgta cgccctcatg gcactgtcag gccaggagta
     5101 cgtggacctc tacgcggcgg acgtcaagaa cgccatggac gcggagaaga agaccgccga
     5161 ggaggtggtc acccagatgc tggctgctca ggcgcaggcc cgcccctcgg ccgccccggt
     5221 ggcggctccc ggtgggcgtc ccgcccccga cgtcgaggtg cccctgcccc cgcaagagga
     5281 ccagggggag accgaggaga gccaggacga ggtcggccgc tcagggcagc aggacgcctc
     5341 ttctgctcct gggggaggct cctcttccgc agaggaacct cctgcgccgg agtgaggcct
     5401 ctggctccct gcccggccgc cccgtgctgc tgcgagccat cctgccccag ggccgtggac
     5461 cgcatcgggc tgtgcccgtg acgctaggat gtgcctgtcc gcctcacctc ttacgaaagg
     5521 cccctcatgt caatccgtcg cgtcgccctg ctgaccgcag gcggcttcgc cccctgcctg
     5581 tccgccgctg tcggtgacct tgtcgagcgc tacacccagg tcgctcccga ggtggagatc
     5641 atcgcctacc agtacgggta ccacggcctg ctcactggca gctacgtcgt cgttgacgag
     5701 gagggccgca ggaacgcggg catcctgcgt gagttcggcg ggtcacccat tggcaactcc
     5761 cgcgtcaagc tgaccaacgc caagaacctc gtggagcggg gcctggtggc cgagggcgtc
     5821 gatcctctag ccttcgccgc cgagcagctg cgcaaggacg gggtggacgt cctgcacacc
     5881 attggcggcg acgacaccaa caccaccgcc gccgacctgg ctgcctacct ccacgacaac
     5941 gactacgagc tgaccgtggt tggcctgccc aagaccatcg acaacgacgt cgtcccggtg
     6001 cgccagtccc tgggggcgtg gacagcggcc gagcagggcg ctgagttcgc cttcaacatt
     6061 atcggcgagc accgctccaa cccgcgcatg ctcatcgtcc acgagtgcat gggccgcaac
     6121 tgcggctacc tcaccgctga gacggcccgc cgctaccgcg agctgctgga gaccaagcag
     6181 tgggcaccgt ccctggggct gagcaaggag cgctgggacg tccacgccgt cttcgtcccc
     6241 gaggccaggc tggacatcgc caccgaagcc gagcgcctca aggccatcat ggacgagcag
     6301 ggcaacgtca acatcttcct gtccgagggc gccggcgtcc cggagatcat cgcggagatg
     6361 gaggctgcgg gccaggaggt ccagcgtgac cccttcggcc acgtcaagct ggacaccatc
     6421 aaccctggcc agtggttcgc ccggcagttc gccgatctca tcggtgcgga gaaggtcatg
     6481 gtccagaagt ccggctacta ctcgcgtgcg gctgccgcca acgcggagga cctggccctc
     6541 atcaagcaga tgtgcgacca tgccgtggac tgcgccctgc gcggcgtctc cggcgtcatc
     6601 ggccaggacg aggagaacaa cgacgagctc ggcgccatcg ccttcccacg catcgccggg
     6661 gccaagccct ttgacatcac ccagtcctgg ttcactgagc tcatgactga cctgggccag
     6721 tccgtggagc ccgctgaggc ggctgcgcag cactgacccg gctggtaggc caccctgctg
     6781 acgaccctgg cgggaccccg cacggtgcgc ggcacctacg cggcagcgtg cctgcacggc
     6841 cgggtgtccc gccaggtggc cgtcatgtgg acagcgcagc tggctcacct atgctggctg
     6901 ccgtgatgaa cgaccttgac gcgccccgta gccacggccc cgcctggcgg gatcggcctg
     6961 ccttccagca gcctgtctac ccggacgcgg ctgccttgga tgccgtgggt gctgacctgc
     7021 gctcgcgccc tccgctggtc ttcgcggggg aggtggacgc cctgcgctgc cagatggcgg
     7081 ctgcggcgga cgggcgtgct ttcgtgctca tggggggtga ctgcgcggag tcctttgccg
     7141 acacctcggc cacctccatc cgcctcaagg tccagacgat cctccagatg gcggccgtgc
     7201 tcacctacgc ggcctccacc cctgtggtca agatcggccg tatggccggg cagtacgcca
     7261 agccccgcag cgcggacacc gagacccgtg agggcgtgac cctgccctcc taccgggggg
     7321 actcggtcaa cgaccacgcc ttcacgcccg aggcccgcac ccctgacccg gcccgtatgc
     7381 tcgaggccta cctgcgggcg ggcaccacgc tcaaccttgt cagggccttt acgatgggcg
     7441 ggtacgccga cctgcgtgag gtccacctgt ggaaccgggg gttcactgcc aaccccgcct
     7501 acaagcgctt cgagtccttc gccgaggaga tcgaccgggc catgcggttc atggaggcgg
     7561 cgggcgtgga cttcgaggcc ctgcgccagg tggacttcta ctccgcccac gaggccctgc
     7621 tcctggagta tgaggacgcc atgatccggc aggactcgcg taccggtcgg ctctacggca
     7681 cctcggccca catgctgtgg gtcggggagc gtacccgtgg cccgcgtgac gcccacgtgg
     7741 ccctcctgga gggggtgcgc aacccggtgg gggtcaagct cggccccacc acgacccccg
     7801 aggaggtctc ctccctcatg gaccacctca accccgaggg cgagacgggg cgcctgagcc
     7861 tcatcacccg catgggcgcc gagcgtatcc gtgaggtgct gccgggcctg gtcgaggccg
     7921 tgcgtcgcga cgggcgcccg gtgacctgga tcaccgaccc gatgcacggc aacaccatca
     7981 cttccgacaa cggctacaag acccgccgct tcgacacgat catggacgag gtccgcggct
     8041 tcttcgaggt gcaccgcgac ctggggaccg tcccgggagg agtccacgtt gagctcaccg
     8101 gcgacgacgt caccgagtgc ctgggaggcg gggagcacat tgacgagcag cggctggcgg
     8161 agcgctacga gaccctggtg gacccacgcc tgaaccacca gcagtccctg gagatggcct
     8221 tcgaggtcgc cgagatgctc caggaccggt ccaggaccag ctaggagcgg tccaggacca
     8281 gacccatggg cgcgtggtac ctgtgagctg gtgaggggcc gcttaagagg ctggtgcggg
     8341 gccggtcaga gcacggtgac ggtgaccgtg ctgcccaccg tgacgctggt accagccgga
     8401 ggctcgatgg aacgcacctt tcccaggagg ccgcccagga cacggctgac ctccacgccc
     8461 aggcccgcct cctccagcgt gtccgtggcc tcctcctcgc tcatgcccac cacgtcgggc
     8521 acgtcaaccc gctgccttcc ccgggagacg acggctgtca ctgcccggtc ccgggtcacg
     8581 cgctctcccg cccccggcgt ggaggagatc accaggcctg cggccaccgt gtcggagtag
     8641 gcgcgtactg ggctggccca gaccaggccc tggccctcga ctgcggagtg ggcctcctgc
     8701 tgcgtcatcc ccacgatgtc ggggaccgtg atgcgccgtc ctggaccgac ggtcctgtac
     8761 caggtggcgt agcctccccc tgccagcacc aggacgccgg ccccggtaag cagcgcccgt
     8821 cggccgtagc ggcgcctggc cgagtcctgg gaggtcgctt ctgagggtac ggggacggtc
     8881 tgctgggtcc ggtctcccgt gagaagggac ctgcggggga gctggcgggt gccggtttcg
     8941 gcgggctcgg ggccgatgga gccgattggc agggacacgg tgcgcatgcc cgcgctgggg
     9001 gtgtcccgct cctggtcagg ctcgggggcc tcggccatgg ctgagcgtgc tgcgcgcgcg
     9061 ttggcagcca tgacctcatg ggtccgctct gagccggtgc ccccgccggg acgcacggac
     9121 aggtcggagc cggtgagctc tcccgccacg tggcgcagca gtgtcagggc ggcgtcggcg
     9181 tcgccgggcc gctggagggg gtcgcgccgg gtcatggccc gtaccagctc gtcgacgttg
     9241 gccgacatct gcgggaccgt cgtggaaggc agggggacat cctcgtggac gttgcggaag
     9301 gcgacctgga tgggggagtc ggcgtggaag ggcaggctcc cggtcaggag ctcgtagagg
     9361 acgacgccga cggagaacac gtcggcgcgg gcggttgagg cgccggaggt gatcagctcg
     9421 ggtgccaggt aggcgacggt gcccaggacg ttaccggtcg tggtctgtgc cacctcggtg
     9481 acggcccggg ccagcccgaa gtccgcgacc ttggcgacgg ccccgtcggc aggcaggagg
     9541 acgttctcgg gcttgatgtc gcggtggacc aggcctgctc ggtgggcggc ccccagtggg
     9601 cgcagcacct gggccaccag ccccagcgcc tccccgacgg acagcgggcc ggcggcgatg
     9661 aggtcccgca ggctcgggcc ctcgacgtac tccatgacca ggtaggccac gccgtccagg
     9721 ctgccctggt cgtagacggc taccaccccc gggttggaca ggcgcgcggc cgcgcgagcc
     9781 tcacggcgga agcgtgcgac gaactcgggg gagtccgcca ggtgggcatg catgagcttg
     9841 agggcgacgt cgcggtccag gcgccggtcc tgcgcccggt agaccgtggc catgccacca
     9901 cgagcgacct gggcgacgat ctcgtagcga gagtcgacca ggcgaccgat gaggggatcc
     9961 ttgaccacgg cgtgattcta ggatgatcgc ggaggcgggg tcaggtactg cgctcggtga
    10021 ggaccagggc cagggtctcg aggtcctcca ggcaggcagg gctgaggccc tcctgggcgc
    10081 gcaggcgttc cagcgcctgg tagccggcct gccggtgggc ctggatctgc tcctcgtggg
    10141 ctgcccgggc accgcagtcc tcaatgacct gggcggcgcg ggcgatctcg gcctcaccgg
    10201 ccgcacggtt ggccaggacc tgctccagga cacgcctgcc cgcgtcgtcg gtgcgccccc
    10261 aagccagcgc cagcatcact gtgcgtttgc cctcgcgcag gtcgtccccg gcgggcttgc
    10321 ctgtcagtgc cgggtcaccg tagacaccca ggtcgtcgtc gcgcagctgg aaggcgatac
    10381 cgatctcctc gccaaagtcc cgcaggtggt ccaggagggc agagtcgggc cgtgccccgg
    10441 ccaggagcgc cccgatgaca agggggtgct gcacggagta ccgggccgac ttgtgccgga
    10501 ccactgacag ggagctggcg tgcatcctgg ccgaggcgcg cagggggtcc tcctgccggg
    10561 gcaggggcag ggcctgggag cgtacgtcca ggaactggcc cagggcgacc tcggtcgtca
    10621 tggcgtcgaa ggccgcgcgg gcgtgctccc ccgcgaccgt gtccgccagc gggaggacgg
    10681 cggtggcgcg tcccatctcc tccccggcta gcgacagcag gaggtctccc aggaggatcg
    10741 ccgcgctggt accaaagtcc tcggcgtctc ccagccagtg gttagccgcg tgggtgcggg
    10801 cgaacaccct gtgggatgcg ggctggcctc gccgcctggg cgccgcgtcc atgacgtcgt
    10861 catggaccag cgcgctggcc tggtagagct ccagcgcggt ggccaggtgg acggactggg
    10921 ggctggtcag tacctggggc cggagggtgt gcggtgccag gaggccggtg ccgacggctc
    10981 ccaggatggc ccgcatgcgc ttgccgccgc caagagcctg ggacgccgcg tcgagcatgt
    11041 cccccccggc gtctcccagg tcgtcccacc ggtgacggca gtccgccagc agctggccga
    11101 gccgggactc cacggcctcg cgtacgacgg tcagcggctc gggaaggtca ggcatgggca
    11161 cagggtagcc ggacaggggg tgcgggggag gcgcccaggc cccggaggcc agcccgcctc
    11221 ggctgggagc aggccgggga cggggtcttc cgagcggcgt cctggggaat aaacgctaca
    11281 atctgtcagt tgcttgtccg tagcgacagt tgggcagcag ctgacggctg tggcccactg
    11341 cctgccgtaa gcctgctcac gcgaaaggag ccctgtggct tccacactga cgcgtacccg
    11401 cgtcgcgctc accgacgacg tcaacgagac tgctgatgac ttcgaccttg acgatgaggg
    11461 agaggaggac ctggacgagg tcccggacaa ggactctgag gactccgaca gtcctgatga
    11521 ctctgacgac gaccctgcgg acgactcggt ggatcccggg aagggttccg aggaccctga
    11581 gggcagggcc gtcacagggg ctgacgacca ggaggagccg cgtcccgagc tgcgggacta
    11641 ctaccttgac gtcaccctcg agaccgacgg tgacggctcc aagcgcaacc ctttcaacac
    11701 ccctgcctcg ctcaaggggg tgaggctggc tcccggggct acgctgttca tccgctcccg
    11761 gaccgtggtc gaggacctgg agatcgctgg ccacggcacc attgaggagc ccatcacgct
    11821 ggctccctac gggcacggcc ctgtgccgcg atttgtcgtg gacggctctg agcccatggt
    11881 cgcgcgcgag ttcctcgccc tcaacggcta catcttcaag gggtgggtca tcaagggcat
    11941 caagatgcag ccctccatcg ccgccctgac cggggagctg gagcggatgc gtcgtgagga
    12001 ggcggagaag gctgccgcca agaaggccgg tcgacgctcc aggtcggggg acaaggacgg
    12061 ctccttcacc gtgtcggaca gcgacgaggc ggacgagccc gcccagcgcg tcgtgacagc
    12121 aggcgccacg gcggatccgg tcaaggacta cctcaagcag atcggcaagg tggctctgct
    12181 caacgccgag caggaggttg agctggccaa gcgcattgag gcgggcctgt tcgccgagta
    12241 ccgtctcagt gaggagggca atgaccttcc cgccaggctg cgccgtgagc tgcactgggt
    12301 ggcccaggac ggccagcggg ccaagaacca cctcctggag gccaacctgc gtctggtggt
    12361 gtcgctggcc aagcgctaca ccggacgcgg catgctgttc ctggacctca tccaggaggg
    12421 caacctgggg ctgatccgtg ccgtggagaa gtttgactac accaagggct tcaagttctc
    12481 cacctacgcc acctggtgga tccgccaggc catcacccgg gccatggcgg accaggcccg
    12541 caccatccgc atcccggtcc acatggtgga ggtcatcaac aaactggccc gcgtccagcg
    12601 ccagatgctg caggacctgg ggcgtgagcc cacgccagag gagctggccg tggagctgga
    12661 catgaccccg gagaaggtgg tcgaggtcca gaagtacggg cgtgagccga tctccctgca
    12721 cacccctctg ggagaggacg gcgacagcga gttcggtgac ctcatcgagg actccgaggc
    12781 cgtggtccct gccgacgcgg tgagcttcac cctcctgcag gagcagctcc acgccgtcct
    12841 ggacaccctg tcggagaggg aggcgggcgt ggtctccatg cgtttcggcc tcaccgacgg
    12901 ccagcccaag acccttgacg agatcggcaa ggtctacggg gtgacccggg agcgcatcag
    12961 gcagatcgag tccaagacca tgtccaagct gcgccacccc tcgcgcagcc aggtgctgcg
    13021 cgactacctg gactaggcag ctggactagg cgaagaggcg acggcgaggt gacgtaccgg
    13081 ccgcagcccg gggggatggc cctctggctg cggccgggcc tggttgaggc gctgacgtcg
    13141 tcacgctcac cgtggccggg gtgcgtgccg cgtcaaggtc gtgcggctca ggtagttact
    13201 atcgggcacg ccccgctgtc agattgctgc gtcagcagct gtcgtgcctc tgactgccct
    13261 cttcagggta gggcaccgcg atgaaagtgg tcgacgagtt ccggaccgct tgtcacgaag
    13321 gtaggtggca gttgggtgcc tggggtgggt cttgggtctg ggggttgtgg tggtggtggt
    13381 gtggtggggg ttacggtttg gtcatgcgga tgagtgagca ggagtggcac gaggcgtggc
    13441 tgcgcgccac gcctgagtgg gtggacgcga acatggacga cctggtgaag gaggggtacg
    13501 cgccgatggg tgtgggtgcc ccggtcaccg ccgagcacgt gggctacttt ggtggggtgc
    13561 tgcctgcctc ggtgctgcac ctgtgggagc ggttcggctt cgatggcttc ggggcggggc
    13621 ggtggtggtt caccgacccc taccggtggg cgccggtggt cgaggcctgg ctggggggca
    13681 cggccatacc gttcccccac cagcggtggt ggtgcctggc ccgctccgcg acagggacca
    13741 tgaggctgtg ggggggaggt ctccggcccc gctctgaccc tcacccccgc cctggggtgg
    13801 gtgcgtcccg acgcagtcag cgcctccagg atgtcggacg cggtgatgcg tgagcgcatg
    13861 ggcgccacca ccctggactc gccacgcaag gacctgggcg acgacgacac cggggtgctg
    13921 gtggccgact gggccatcga caacctgggg gtcctggggg ctgaccaggt ctacgggctg
    13981 accccggcct actgcttcac cgggcgggtc agcaccagcc aggtgggtat cgaggacgcg
    14041 gtcgcgcacc tcacttttct ggcccaggcc caggagcaca ccctggtgga ggacttctcc
    14101 gccgccgccg cacaggtcgc cgccaccatc gccacaggcg cccaggacaa cgggtcgggc
    14161 agcgccggtg gccccggtga cgacagcggt gagggtgccg gtggtcccca cggcccggag
    14221 gcgcgcggac cgcaggaggg gtcctgatgg ccgggcgctg gcgggaccgg ccccgctggc
    14281 tcagggacgg gcgcccaccc acccccgccg ctggtcccac aggccgcagc ggtggtggcg
    14341 cggcccccga ccacagcggt ggtggtggtc aggtccctgg ctgcggcggt ggcgggggcg
    14401 cgggtaccgc cttggtggcg tctggcatgt tcaccccggg cagggctggc gggcagagcg
    14461 tgctggtgtg ggagggggcc gtggcggcac cggcgagccc gctggcgtgg cactcggcca
    14521 cgccggtgga ggtgttcttc cacaccggcc accacgactc cacccaggtc ctggtggctg
    14581 agttcacccg ccagctggac cgacagctct ccgccctgtc cctgctcagc gcgcagcgcc
    14641 tgctggaggg catccgcgcc tacagggcac atggccgcca gcccacctcc aagaccacca
    14701 ggaataggcg caagaagttc atggaggagg tcgtgggcga cctggtgcac gtgcaccgcc
    14761 tctcctggga ggacgcccag gccgcgacgc gggaggtgga ccgggcgctg gtggtgctcc
    14821 acgagtccga ccaggtcacc tacggcccct cccagcccgc caccaccacc gaggggtggc
    14881 cctccctggg ccacggggtg gtcagctcct ccatcggcgg ccagggcagg cccgtggcca
    14941 cggtcctgga gcaggccacc ctggccgtcc cagccgacca gaggccgcgt gtgcgcctgc
    15001 tggtgcgcgc ggtgctgacc ggctcccccg acctggccca ggacctgcgc cacggccagg
    15061 tggtgctgga gccgcgcacc caggcccagg tgaacgctac cagcccccgg gggccaccat
    15121 ggacacccag ccacctcgcc gccaccatcg ccaccggcca caccccctgg cacccaccac
    15181 ccgcccgggc acaggagccc gaccaggcgc aggagcccgt gctctcggcc acgacccagc
    15241 ccggggccgg gcagccccac cagccagacc ggcccggccc cgccaccacc cagccgtcgc
    15301 cggagccacc ccggccaggc cccacccgca ccggcgccac cacgcccggg ccaccccaca
    15361 cgggcaccac cacgcccggg ccgccccagg cgggcgccac cacagccggg ccgggccagc
    15421 cgccccggcc gctgacccgc gaggaggtcc tggccaacat cgcgtcccac gtggcccagg
    15481 cggcccgcca gggcgaccag ccccacaccc cacgccaggc ccgggccgca cgggaggcac
    15541 ccgccggggc cgcccgcgct gcccgcctgc gcgaggcctc cttcccccac ccgcccaccg
    15601 ccgccgtacg cacaccacca gcacagcccc aggcaggcac cacccggaca ggacccgccc
    15661 cgggccagcc gtcccggccg ctgacccgcg aggaggtcct ggccaggatc accgcccaag
    15721 ccgcccagca gcccacccag ccccgcaccc cacgccagac ccccaccaga aaccggaggc
    15781 gcaaccacgg cctgggccgc taagacccga cccacacacc cgcacacacc ccgcaggccc
    15841 agggccgacc ttcccgtccg ccgctcccgc ctgcccggag cctgatggcc tgctcgagcc
    15901 ttccccggga cggtgtgcga ggctcgggta cggcagggga gcaccggggc gtcgctgcac
    15961 gtacccgaag gtcggaaagc ggaaaaatgg aaaccgaatg agagtggtcc cccggcctcc
    16021 tgggccgggg gaccatggac gtcagtgctg gactaggctg ggccagtcag tcctcggaca
    16081 ggaggcggtc ggactcgtcc tggacgaaca gggcgctggc ctgcagggcc tccgcgtgcg
    16141 cccggccgtg gtgggcgcag aagaggagct cgccggaggc gaggaccacc cggacgtagg
    16201 cctgggcact acaggcgtca caccggtcag cggtcgtgag gggacgcctg gtctggtcta
    16261 cggaggtggt ggcggcaggg gccgccgtcg cggtcgcctc cgtagcggtg gaggcggtcg
    16321 tgctcgttgc cttcatgacc ccatgacatc acgtccagcc aggcggctcc tacccgggag
    16381 gcctccttcc gccccgggcg cagcacctgt gcccacggcg aacggtggga atccgggcgg
    16441 tggacgacct cctgcgtcag gctcgccctg cgggtcggcg tccagcggag gtgtccctgc
    16501 tccacgcttc cactgctcgg ctcggtcccg gcagcgtggg tgcgttccac cttcctgtgg
    16561 cgaggaagaa cgcctgttcg accctcatcc gctaaggttc gcccgtgcct gcatccaaga
    16621 ccgcgaccag ccgcgcggac aagtctgcca ggtctgagca gcctgctcag acctcctact
    16681 ctgcccgtca cctctccgtc ctggaggggc tggaggccgt gcgcaagcgc cccggcatgt
    16741 acatcggctc cactgaccag cgcggtctca tgcactgcgt ctgggagatc ctggacaacg
    16801 ccgtcgacga ggccctggag ggtcacgggg acgacatcac cctggctgtc cacggcgacg
    16861 ggtcgatcga ggtgcgcgac aacggccgtg gcgttccggt ggacgtcgag ccctcctccg
    16921 ggctgacggg ggtggagctg gtctacacca agctgcacgc gggagggaag ttcggcgggg
    16981 gctcctacgg cgccgcaggc ggcctccacg gggtgggcgc ctcggtggtc aacgccctgt
    17041 cggcccgcat ggacgtggag gtggaccggg gcggacggac ctacgccatg agcttccgcc
    17101 ggggcgagcc cggggtcttt gacgactccg ccggacgcgg cccgcagtcc cccttcaccg
    17161 gtttcaccca ggactcacag ctgcgtgtgg tcggcaaggt gagggcaggt gtgaccggta
    17221 cccgggtccg ctactgggcg gacccgcaga tcttccccag gccgagcgag ttcgacgccg
    17281 gggcactgcg agcccggttg cgccagacca gcttcctggt ccccgggctc accctgcgcc
    17341 tggaggacca ccgtccccag gctgaggtgc tggccgcctc caccacccct gctgctgacg
    17401 ccggcagcac cgacgacgcc gtccacgagg cgtccctgcg ggtggcggcg cgggtggctg
    17461 cgccagtcac gacaggcact gctgccactg ctgccggcac agggaggggc accagggcgg
    17521 gtgccggtga cctgttctcc gccagtgggc aggacagcag tggggacgtc aacggcagtg
    17581 ctcctggcag cggggcaggc ggcgccgagg tcttctacgc ccggggcggg accgctgact
    17641 tcgtggactt cctggcctct gacggctccg tgacggacac cttccgcctc acgggccagg
    17701 gcacctacac cgagaccgtg cagcagctgg acgcgggcgg ccacctgcgc ccggtcgagg
    17761 tcgagcggac ctgcctcgtg gacgtggccc tgcgctgggg agttggttac gacaccacag
    17821 agcgttcctt cgtcaacatc atcgccacgc cgatgggcgg cacgcacctc agcgggttcg
    17881 agcaggccct gacccgggtg ctgcgcaagc gcattgaggc ggactcccgg gcgttgaagc
    17941 tcacctcccg cgacgggcgg gtcgagaagg acgacatcct ggcgggcctg accgccgtgg
    18001 tgactgtgcg cgtgcccgag ccccagttcg agggtcagac caaggaggtg ctgggcacgg
    18061 ctcccgtgcg ccagatcgtg gccaaggtgg tggacaccga gctgacggcc ctgctgacct
    18121 cctccaagag agacctcaag gcccaggccc gctccctgga ggagaagatc gtcggggaga
    18181 tgcgtgcccg ggtctcggca cgcctccaca aggagatcac ccggcgcaag acggccctgg
    18241 agacctcctc cctgcccgcc aagctcgccg actgccgcag tgacgacgtg gcggcctccg
    18301 agctgttcat cgtcgagggc gactccgccc tgggaacggc caagaacgcc cgcaactccg
    18361 agttccaggc gctcctaccc atccgtggga agatcctcaa cgtccagaag gcctcccagg
    18421 cggacatgct gcgcaacgct gagtgctcgg ccatcatcca ggtggtcggg gccggctcgg
    18481 ggcggacctt tgacatcggg gcctcacgct acggcaaggt catcctcatg actgacgccg
    18541 acgtggacgg cgcgcacatc cgcaccctcc tgctgaccct cttcttccgc tacatgcgcc
    18601 ctatgatcga ggccgggcgg gtcttcgccg ccgtcccacc cctgcaccgt atcgaggtgg
    18661 caggcagggg gcggcgtaag aaggaggtca tctacaccta ctccgacgag gagctggtgg
    18721 agaccctgcg gcgcctggag cgctcggggc gcagcttcaa ggagccgcag cgttacaagg
    18781 gcctggggga gatggatgcc caccagctgg ccgagaccac gatggagccc gagcaccgta
    18841 ccctgcgacg catcaccctg gccgatgagg cggaggttgt ccgggcggag tccgtcttcg
    18901 agctcctcat gggcagctcg gtggagccgc gccgggactt tatcgtccag ggtgccaggg
    18961 acgtggaccg ggaacgcatc gacgcctgag aacctccggc gggcggtggc agtgaccggg
    19021 ctggtggtga cgtgtggtgc gccgagtatg gcgcactgag caatagtgcg ctgagcaggt
    19081 gggacaggct ggtacgggcc gcaggcaggg aggttgtggc cgcagtccag cgggcctggg
    19141 gtaggctctg ctctggccct gccctgccag ccctgatagc cccggacacc tctgacaggt
    19201 ccggccctac ccgaaggagg gcgtcttatg gctccagtct atggttcccc tcgcggcggg
    19261 ggctggtctc cgtggaccat gctgcgtccc ggaccccgtt cctcgctgtc gcggcacctg
    19321 tcgtggatgc cgctgagccc tgaggacaac gccgagctcg cagctctcat ctcccgcgca
    19381 gagacggtgg acgaccctcc ctaccggact accgcccagg agaccgccga gtacttcatc
    19441 gaccccacct actctggtgt cgcaggtcgt gacgaggagg gggtcatgcg cgccttcggg
    19501 ctggtgcgtc tgcgccctgc cggggagatc tacgtctcca tgaccggggt cgtggacccg
    19561 agcaggcgcg ggcaggggat cgggcgctcg ctgctgcact ggcaggccga gcgggcgcgc
    19621 caccttgtag gggccgagcg ggcggtctcg ctggctgact ccgccgcttc ccgggcgcgg
    19681 cgccagccag cccacgtggt caccacggtc ctggagggtg acgagcggat gcaggagcac
    19741 ctgaggcacc tggggttcga gcctcgccgc tggtaccgtg agctgcgtcg ctccctgtcc
    19801 ctggacatcc cggtggtcga gctcgacggc ttcctacggg tcgagccgtg gacgcccgag
    19861 attgatgacg ctgtgcgccg ggcgcacaac caggccatag gtgagggctg gggctcgcgg
    19921 agcctgaccc ccgaggagtg ggcgcagggg agtgcctact tcgcgccccg ctggagcttc
    19981 gtcgccacgg accgctccgg cgaccgtgcc cgggtggctg gctaccttcg ctccagccgc
    20041 tacgagcagg actgggaggc gctgggctgg agggagggct acaccgacat gctgggtgtg
    20101 ctgcgtgact accgcagccg acgcgtgggc tcggcgctgc tgtgtgccgc catgcaggcc
    20161 tacgcggctg acggcatgga gtacgcggcc gcaggcgtgg acacggacaa cccttcaggg
    20221 gcggttgacc tctacgagtc cctgggctac gagcccacac gtgggacagt cctctacgtc
    20281 ctagacgtgt gaggagggtc ctggacgtgt aggcggctag cgtgccaggc tcgggccgtg
    20341 tgctggcgcg ctgcggggcg ggcaggggct ccagccaggc acacagggag gcaggcgcac
    20401 tcggggcgct catcagctgg tacccgcagg tcagccgact ccggtaaccg gcgaggctgg
    20461 ctgggtcccc gagccgtccc ggcgctcgtc aggctccggc agggatgtcg gcctgccgcc
    20521 tgaacgcagc gccctggtgg gagccacgcc gacccacgcc agcaccagct tgtgctcccc
    20581 ctggaggaaa cggtgcgtgc gcactcctgc cgtggcacgc cccttggccg ggtagcggtc
    20641 caggggagtg accttgacgc tgcccccacc ggtgccgggc agcgtctgtc cggcaccggc
    20701 gacggtcacc accacggcct cagccaccag ctcctcaggg acggcgccac cgaagaccac
    20761 ctgggcctcg gggcgcaggg acattcctgc cattccggcg gcgccccggc cctggggccg
    20821 gaccctggcg gcctggaagc gcagcagctg ggcgtcggag gagaccaggg cgaggaagtc
    20881 ggagtcggcg gcagtggtcg cgcacaccac ccggtccccc tcggacaggg agatgatcgc
    20941 ccaggcctgg ccgttgcggg gctcgtcccc ggggcgtacc cgcttgacca cgccctgggc
    21001 cgtcatgagg accacggggt cggtctgggc gccacccacg gggaccagtc cgaccacctt
    21061 ctccccggcg gcggtgtcca ccagctggtg caccggggcg cagccagcca gggagggtgc
    21121 ctcctgggtc cgctcaatct ctggcacggc gacca
//
