LOCUS       NZ_CP025227            21110 bp    DNA     circular CON 14-APR-2024
DEFINITION  Actinomyces wuliandei strain 299 chromosome, complete genome.
ACCESSION   NZ_CP025227
VERSION     NZ_CP025227.1
KEYWORDS    RefSeq.
SOURCE      Actinomyces wuliandei
  ORGANISM  Actinomyces wuliandei
            Bacteria; Actinomycetota; Actinomycetes; Actinomycetales;
            Actinomycetaceae; Actinomyces.
REFERENCE   1  (bases 1 to 3107761)
  AUTHORS   Zhu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2017) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No.155 ChangBai Road, Changping District, beijing
            102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. HGAP 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004010955.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 19:14:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,606
            CDSs (total)                      :: 2,544
            Genes (coding)                    :: 2,496
            CDSs (with protein)               :: 2,496
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 18 of 48
            Pseudo Genes (incomplete)         :: 37 of 48
            Pseudo Genes (internal stop)      :: 3 of 48
            Pseudo Genes (multiple problems)  :: 9 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:15:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 565172
            Orig. end                         :: 586282
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21110
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="3"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21110
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21110
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11110](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11110
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            1113..2579
                     /gene="nuoH"
                     /locus_tag="CWS50_RS02245"
     CDS             1113..2579
                     /EC_number="1.6.5.9"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nuoH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005870465.1"
                     /locus_tag="CWS50_RS02245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="WP_127841492.1"
                     /transl_table=11
                     /translation="MTTTQILATATQPGAGGVAADFSQETWWLTLIKAVFVVAFLIVSV
                     LMVLWVERRGLGRMQTRLGPNVNGPFGLLQAVADAGKLILKEDFWLRGAERAVYLLAPV
                     IAALSAFMVYAVIPFGPQVSILGHSTPLQLTDFPASVLYILAITAFGVYGIILGGWSAH
                     STYPLFGAVRSAAQVISYELSMSLSILTVFLVSGTMSTSGIVSAQERVWWAVAMLPSFV
                     IYVISMVGEVNRLPFDLPEAEGELVAGHMVEYSSMKFAWYFLAEYINMFNVSAVCVTLF
                     LGGWRSVLLASFWEGANSGWWPMLWFVAKVWVVMFLMVWTRGTLVRIRYDQFMRLGWKV
                     LIPVSLVWFVLVAVVQAYRTFSGASVQSLLLVLAAVFLVAMVVLFLLPDDQGEQDDGVE
                     EPQLLGARTGDDGDLAEVTVPGEEHLAFAGGYPVPPLPGQHLPPSPRARRGGERGQDSH
                     LPSHRSSQDAAALPASTTNLAADAQEGPDD"
     gene            2572..3303
                     /gene="nuoI"
                     /locus_tag="CWS50_RS02250"
     CDS             2572..3303
                     /EC_number="1.6.5.9"
                     /GO_function="GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /gene="nuoI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017178989.1"
                     /locus_tag="CWS50_RS02250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="WP_127841493.1"
                     /transl_table=11
                     /translation="MTDQPTASRGTSGPRPQGGSDHDQWRRDAPSLLAQVFAPVAGYGV
                     TISSMFRPVVTEQYPREPAQVMPRFHGRHRLNRYDDGLEKCIGCELCAWACPADAIYVE
                     AASNEPGAQYSPGERYGRVYQINYLRCIFCGMCIEACPTRALTMSTDFDELVGPTRTGL
                     IYDKDDLLVPVPAGAVAPPHPMVEGTEDGDYYRGAVTGPTQAQIDWVRQARPQDSTLST
                     ARPVAGDGHGSPGNEPVREAV"
     gene            3303..4442
                     /locus_tag="CWS50_RS02255"
     CDS             3303..4442
                     /EC_number="1.6.5.9"
                     /GO_function="GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017195664.1"
                     /locus_tag="CWS50_RS02255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="WP_127841494.1"
                     /transl_table=11
                     /translation="MSLLPLSVSVLVAAVPSAVSTSSSTLPHTVAAAVPTALSEAGTLS
                     VGESILLAVVALVTVACGLGVLTARRAVVAAINMIGIMISLAVLYIANEAPFLGMTQVV
                     VYTGAVMTLVLFVIMLVGVGGEEPVAGAGGSYQLPLIGLLGLGLATLLAAVLHRSPLPA
                     AAGLQGGEASTPDRIAEVLLGDHVVTMELTGTLLVVAAVGALTLTHRQRVRARRGQAET
                     AGEKMRAYAATGAHPGQKPVPGVYASTNTVAAPALGADGHEVAESVPRVLRARGQGMEL
                     SEVSPEMGAAQRAGEVLERQAGSRSTGTDAGDGSVHGSAVGRSGMPSMPGQAAPVVDQP
                     VAPSSALRDTSQSPALGQGAAEGAEGTEEDTEEKEKGDQ"
     gene            4439..4744
                     /gene="nuoK"
                     /locus_tag="CWS50_RS02260"
     CDS             4439..4744
                     /EC_number="1.6.5.9"
                     /GO_function="GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /GO_process="GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene="nuoK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394285.1"
                     /locus_tag="CWS50_RS02260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="WP_119836644.1"
                     /transl_table=11
                     /translation="MTLPIAAYIVLACVLFTLGALTVLLRRNAIIELMGVELMLNAVNL
                     VLVTFSRLHGDLTGQVFAFFVMVVAAAEVVVGLSIVVSIFRTRRSTSVDDVNLLKS"
     gene            4764..6842
                     /gene="nuoL"
                     /locus_tag="CWS50_RS02265"
     CDS             4764..6842
                     /EC_number="7.1.1.2"
                     /GO_function="GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /GO_process="GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene="nuoL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006547365.1"
                     /locus_tag="CWS50_RS02265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="WP_127841495.1"
                     /transl_table=11
                     /translation="MTVATGIASAVSVAASVPVSPRALTPASPVLAADTVAVAQPATGL
                     AGLSWLLVAVPAASAAVLLLAGRRSDSWGHWLGLLASLASACLGLGVLAQILALPPQER
                     VLSVSLWHWFSSADLSVDVGLRVDPLSLTFVVLVTCVGFLIHLYSVAYMGHDRDRRRFF
                     AYLNLFVAAMLTLVLGDSYIVVFVGWEGVGLASYLLIGFWNTAEADAPRAQREKAVDNA
                     VAAKKAFVMNRVGDLGLLLAMMALVSQVGSVAFDTVLPAAARGEVSSGWLTAIGLFLLL
                     AACGKSAQFPLQAWLGDAMAGPTPVSALIHAATMVTAGVYLMVRSGPVLEGAPQVQVAV
                     AAVGALTLVLGAVIGCAKDDMKKVLAASTMSQIGYMMLGAGLGPVGYAVAVFHLLTHGF
                     FKAQLFLGAGSVMHAMGDQVDMRRFGALYRVMRVTWVTMGIGWLAILGVPPFSGFWSKD
                     RIIEAAFTGEGAQPWVLGTVALLGAGLTAFYMSRLFFMIFHGKARWTTSSDLEGEVHPH
                     ESGWLMTLPLIVLSLFSLGLGGLLSQGEAFTTWLEPVTGHVDHSAHEPVLPVTVIMGGT
                     LALVLLGALAAWAMYARREVPTVVQPGSVMVEAARHDMYQDALNEAVAMRPGQGLVAAT
                     RAAERYVVDGAVEGAAVTASAAGSLLRRTESGYVRSYAGYMLGGTVLALAAVLASRL"
     gene            6857..8431
                     /locus_tag="CWS50_RS02270"
     CDS             6857..8431
                     /EC_number="1.6.5.9"
                     /GO_function="GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /GO_process="GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787139.1"
                     /locus_tag="CWS50_RS02270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="WP_127841496.1"
                     /transl_table=11
                     /translation="MQTLPATLPVLTIMAVLPLAGAVLLWVLPPLRRYASLLGLAVSLA
                     VLAVGVWALSMFDLSQAGTVQLAQTTPWIPSFGAYWALGVNGLGLSMLLLGAALVPLVL
                     LASWKEVPQERQALLTGLVLVLEAFVVVIFSARDLFLFYVCFEAMLIPVYFLVGCLGGP
                     GRQRAALKFLLYSLAGGLVMLLGVVMLAIHTSENQVGFLIDTMVGNLTLSPTMSRWLFL
                     SFFIAFAVKAPMVPLHTWLPDTAEQATPGTSVLLIGVLDKIGTYGMVALVLPLFPEASR
                     WASPVVVVLALVSIIYGGLAAIAQDNLYRLIAYTSVSHFGFMVLGIFSGSQVAATGAMV
                     YMVAHGLSIAGLYLVTGFVARRTGTVSISELGGLARVMPLAAGTFLLSGLASIALPGLS
                     GFVPEWMVLTGTFSVSVPLGVAAVAGVVIAAVYVLLPYQRVFTGAPSPDRTGLGDLEGR
                     ERLVLVPVVGAMLALGLVPAPLTSLLDDVGGQASTAVSQSTAQTTASAGHQPLHAHAPL
                     PEPRKGA"
     gene            8433..9983
                     /gene="nuoN"
                     /locus_tag="CWS50_RS02275"
     CDS             8433..9983
                     /EC_number="7.1.1.2"
                     /GO_function="GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /GO_process="GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene="nuoN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010614081.1"
                     /locus_tag="CWS50_RS02275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="WP_127841497.1"
                     /transl_table=11
                     /translation="MTVTGPVIGWGALTPVLIVLGGGVLGVLVEAFVPRRARLATQTLL
                     SVAAVLASGAALTLRFEQMELQGPSRTASGLLEDPFALSSQAVLLVIGFLAVLVMADRT
                     EVGDGAFAAQAADRPGSAEESESLHAGWTTTEVFPLALLSLGGMMLFGASSDLITLFVV
                     LEMVSLPLYIMAATARHRRLLSQEAALKYFVLGAFASAFLLLGAAFLYGLSGAVDYETI
                     GLAAQGEAGAQDGWLLLAGLVLVCVGLLFKIAAVPFHAWSPDVYQGAPTPVTGFMAAGV
                     KAAAFFALLRFFYTVVGPLGAQLAPFLWAVATATMVLGTVVGVVQKDVKRMLAYSAVAH
                     AGFMLIAIITDNPLVVAPLTFYALTYGVSTVGAFGLISLVRTRHDGAVGAEASDLDSFR
                     GLARRSPWAAGAMTVFLLSFAGIPLTAGFMAKFYLFAAGVLGGGTWLVVVAVACSAVSA
                     FFYVRLVVLMFFHAPRAEDGQDTVVVDSQGMVTTAVTVSVLVTVLLGVFPQAAFGLFA"
     gene            10001..11110
                     /locus_tag="CWS50_RS02280"
     CDS             10001..11110
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009747773.1"
                     /locus_tag="CWS50_RS02280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_127841498.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.6e-53, bitscore:
                     178.9, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGSMPLSAPQLEGRITPALEAVETRLLEVVTSADETINPPTSHL
                     AEAGGKRLRPVLTLLTAQLGDPGLAAGDEVRDAGVAVELTHIATLYHDDVMDEAPLRRG
                     APSAQTVWGNSAAILTGDVLVARASQLVAGLGPRAVLAHAKTFERLCMGQLHETLPRPA
                     GTDPVEHYVQVLADKTGSLIAVSARYGAMLTRAGRRTEHVVEAFGEKIGVAFQLADDVI
                     DIISDSDTTGKTPGTDLREGVDTMPVLLLRQALAAGELDAAGQSILSTLSSADLGDDAV
                     LADVVTRLRSHPVLERTRRMAMEWARDAVEVLGGLHDAVLEGTRQRLAADQVTDPGAVE
                     AAVADARERVSHVRDGMEQFARLLVDRAA"
     gene            11501..12997
                     /locus_tag="CWS50_RS02285"
     CDS             11501..12997
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1285: ferredoxin fdxC"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779898.1"
                     /locus_tag="CWS50_RS02285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_127843167.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.3e-10, bitscore:
                     38.2, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPRPLSVAVIGAGPAGIYASDILAKSGLDVSIDLFERLPAPYGL
                     VRYGVAPDHPRIKQIIVALYKILQRGDIRLVGNVEVGRDVSVAELHEHYDALVFSTGAD
                     TDAPLDIPGAQAPECHGGADFVSWYDGHPDHPRTWDLSAREVAVIGVGNVALDISRVLA
                     KHPADLMTTEIPANVAEVLRKAPTTDVHVFGRRGPAQVKFTPLELRELGKQPGVDVLVD
                     EEDFEYDEASRAALAASNQQRQVVKALEGYAMQEPEDLTAPHRIHIHLFQAPTEVLTDD
                     AGHVTGLRTERTRLAGDGSVVGTGELRDWPVQAVYRAVGYAGSPVEGLPFDHERHVLPN
                     EGGRVIGEDGRPMTGVYATGWIRRGPVGLIGSTKSDAQETVANLVADARAGLLHATAQE
                     DSQVGHAALIALLEERGVPYTTWEGWELLDAYERELGSAWGEVEGGRGLRERVKVVSRD
                     AMTAVSRSTEVPDDLIGQPEADRVPERVAHRLRDAVESVS"
     gene            13109..14224
                     /gene="ald"
                     /locus_tag="CWS50_RS02290"
     CDS             13109..14224
                     /EC_number="1.4.1.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ald"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009395492.1"
                     /locus_tag="CWS50_RS02290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alanine dehydrogenase"
                     /protein_id="WP_127841499.1"
                     /transl_table=11
                     /translation="MRVGIPTEVKDSEFRVAITPAGVHALVQRGHEVRVQVGAGLGSAI
                     TDREYAAAGAVLVEGAAPVWAESELVLKVKEPVACEYEHMRPGQVLFTYLHLAADRRLT
                     EELLSRGVTSVAYETVEADDGSLPLLAPMSEIAGRLAAQVGATQLLRPSGGSGVLLGGA
                     SGVRRGRVAVLGGGTAGLCAAQVSLGMGADVTVFDISPARMRHIEEVSGRQIRTRFSTE
                     LDVAQACAQADLVIGAALVPGARAPHLVTREMVTAMRPGSVLVDVAIDQGGCFEDSRPT
                     THAAPTFEVGQTTFYCVANMPGAVPYTSTYALTNATLPYVTVLADRGWREACTARPDLA
                     RGLTTHEGVLYTRGVGEALGMDTGSVADLLG"
     gene            complement(14287..>14518)
                     /locus_tag="CWS50_RS13385"
                     /pseudo=""
     CDS             complement(14287..>14518)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612349.1"
                     /locus_tag="CWS50_RS13385"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="integrase core domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="RHRCTRPYRPQTGAKVKHLNPTPQRGASPRKAPHQRGRASRRLQR
                     LAPPLQPPPTPHQHRQPDTRTTRTQPHSQVHL"
     misc_feature    14721..14738
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.42"
                     /tool="antismash"
     gene            14737..15123
                     /locus_tag="CWS50_RS02295"
     CDS             14737..15123
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF020461.4"
                     /locus_tag="CWS50_RS02295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GAD-like domain-containing protein"
                     /protein_id="WP_127841500.1"
                     /transl_table=11
                     /translation="MRMSKEEWRQGWLHVTPEWVDANMDDLVKEGYAPMGVGAPATAEH
                     LGYFGGVLPASVVHLWERFGFEGFGQGRWWFTDPFQWAPVVEAWLEGTPVPFPSQRWWC
                     LARNATGDMRLWGRSPAPPWTSTQ"
     gene            15081..15560
                     /locus_tag="CWS50_RS02300"
     CDS             15081..15560
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CWS50_RS02300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="T6SS immunity protein Tdi1 domain-containing
                     protein"
                     /protein_id="WP_127841501.1"
                     /transl_table=11
                     /translation="MGEVSGPALDVDPVMGWVRPDAGHARDMADSVMRERMGAIAMTSP
                     RRESLDDDDTGVLVADWAIENLGVLEAGQVYGLTPAYCLTGRVSLDQVGIEDATAHLTF
                     LAQAQEHTLVEDFSAAAAQVAASIAAGGQTTSQGDGPGGTSSGPGGTGPGPGAGS"
     gene            15557..16819
                     /locus_tag="CWS50_RS02305"
     CDS             15557..16819
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026942.4"
                     /locus_tag="CWS50_RS02305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polymorphic toxin type 15 domain-containing
                     protein"
                     /protein_id="WP_127841502.1"
                     /transl_table=11
                     /translation="MTPGRWRGWLRGRGRPTASAAGPAAPGGGAGGGQAPGPGHSSGGG
                     DGAALVAGDIFTPGRAGGQGVLVWEGAVAAPDSPLAWHSATPVEVFFHTGHHRATQELA
                     SEFTRQLDRQLSALSLLSAERLLEGIRAYRTQGRQPTSKATRRARDTFMDKVVDDLRRV
                     HQLSREDARARAAEVDRALVVLHEPDQLLAGPGGPATTGPQPGYPSLGHGQVNSSVGSQ
                     NKPMVAVLEQAALQVPPGAAFPGAPAGARGADRLPRPGPGPAPRPGGPGAPHPGPGERH
                     QPPGATVDTQPRRRHHRHRPRPLAPTTRAGAETRPDPPGHPGPTARPGPARGTHRGRPL
                     PHHQPARGLLPPPAYRRRARTTTPDGPAVAAADPRGGPGQDHHPAAPHPTPDPHQKTPT
                     QPRPGPLAAGASRVRGWQPSW"
     gene            17034..18299
                     /locus_tag="CWS50_RS02310"
     CDS             17034..18299
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016444521.1"
                     /locus_tag="CWS50_RS02310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="WP_127841503.1"
                     /sec_met_domain="PALP (E-value: 3.6e-45, bitscore: 151.9,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPIGSLVPTHWYNLAADFPEPLPPALHPGTREPLRPEDLEHLF
                     PRELIAQELSTERFIEIPQAVREVYAKWRPSPLIRADRLERVLGTRARIFYKYEGVSPA
                     GSHKPNTAVAQAWYNAQEGTTRLTTETGAGQWGASLALACSLFGMSCEVWQVRSSYESK
                     PYRRYQMEVYGSTCHSSPSELTRAGRDVLAQDPQTTGSLGMAISEAVEVAAASEDVHYA
                     LGSVLNHVMLHQTVIGLEAVAQLRQAGVDQPDVVFGCAGGGSNLAGLSFPFIGRNLTEG
                     TSSRVVACEPAACPTITQGEYRYDVGDVAGTTPMMKMYTLGADFVPPAIHAGGLRYHGM
                     APMVSHAVHLGLMDAVAVSQEDAFAAGLLFARSEGVVPAPESTHAVAAALEHARQARND
                     EVILIGLSGNGVLDLPAYEAYL"
     gene            18399..19268
                     /gene="htpX"
                     /locus_tag="CWS50_RS02315"
     CDS             18399..19268
                     /EC_number="3.4.24.-"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="htpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779909.1"
                     /locus_tag="CWS50_RS02315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="zinc metalloprotease HtpX"
                     /protein_id="WP_127841504.1"
                     /transl_table=11
                     /translation="MNGTNHYNGLKTAVLLGGMWGLLILIGYVLAQGTGSSVWLFVMPL
                     IGVAQTAYAYWNSDKIAVRSMGAIEVSEAQYPQMYAIVRELSTSAGQPMPRLYVAPTVS
                     PNAFATGRDPQHAAVCCTQGILQLLNDRELRGVLGHELSHVYNRDILTSSVAAGIAGVI
                     SSISTMALWFGGGRDRRDGGNVIVVLLLALLAPLAAALTQFAISRTREFDADHDGAVLT
                     HDPLALASALRKLETGVNAAPLAQDPRVEPVSSMMIANPFGSRVRNLFATHPPMDQRIA
                     RLEKMAGY"
     gene            19350..20471
                     /locus_tag="CWS50_RS02320"
     CDS             19350..20471
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009395488.1"
                     /locus_tag="CWS50_RS02320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphotransferase"
                     /protein_id="WP_243118418.1"
                     /sec_met_domain="APH (E-value: 2.7e-15, bitscore: 54.2,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDSTQEVVARTDSAEFASVLRRAAESGRVAGVGAGSVRLTRLGAG
                     ESFTAWLLRPCHEDGRPLRVVRVPRLPAEELPRSLRGEYEALSRVPADLGSHGVALEED
                     SDNALGAPYLVTTYVPGRVLPPWAWTPRLACALAEQVARLHHLLGEAQTAGEGTAGSQL
                     QSGQGSHPGALREAEEVLGWWRSHHPDVLEEPRASCLLDPWWQALASLNPVTVGTPLHP
                     LVHGDVVVTNVVVGPDGVPRLIDWEWAAPGDPAKDLALIGGEVTGGPWYAHLSREAVTD
                     MVSRYVTVRRRLAGSEDADKGRDCEGAGGRAEKAEEHDEQVRRLLVRRDAWELVDRVSN
                     LLYCLSRRGQGNYLRWARELAESLEARLAALPD"
ORIGIN
        1 acgatcctgg tgggggagcg ggccgctgcg gtgccgggcc tgctggcagc cgtggacgcc
       61 ctggccaggg ccaccggcgc ccggctggcc tgggtgccac gtcgttccgg cgaacgtggt
      121 ggcgtggagg caggcttgct gccaggactg ctgcccggag gcaggcccgt ggacgacgct
      181 gctgcccggg cacaggtgga gcaggcgtgg ggcctgggac aggggcacca cctcccctcc
      241 gcaccgggac gtgacaccag tgccatcctg tccgccctgg aggacggcag cctcaagggc
      301 gctgtcgtcg gtggcgtgga cctgcgagac ctccccgacc ccggcctggc ggccacgggc
      361 ctgggtggcg cgggcttcct ggtccagctg gaggtgcgcc gcaccgaggt cagcaggtac
      421 gccgacgtcg tcctccccgt cgcaccggcc gtggagaaga acgggacctt tgtcaactgg
      481 gagggccggg tgcgtccctt cggccaggcc cacgtctccc gcgcgcgcac cgaccgccag
      541 gtgctgggca tgctggctgc ggagatgggc tcggacctgc acgtggacag cctggaggct
      601 gtccacgcca cctacgccgg gctggggctg tgggacgggg cacgcccggg tgccatcacc
      661 gtccctggaa cccaggggcg gcccggggac ggcagccccc gctcctcgtc accgtcgtcg
      721 gctcctgctg ctgtggacgc tgcggaccgg gccgcaggct ctgcctcagg ggacgtggtg
      781 ccaggcagcg gcctgcctgc ggtgctggcc actcacaagc ccatgctgga cgccgggcgc
      841 ctcctggtgg gcgagccctt cctggctgcc accgccatgc gcccagtggc acgcctggga
      901 gcagaccttg ctgccgggct gggggtggtc ggtggtgagc ccgtcacggt ctctacgatg
      961 gcgggggcga tcacgctgcc ggctgtcgtg ggtggtgtgg ctgagggctc cgtgtggctg
     1021 ccggagtgct cagcaggctc tgtcgtgcac cagaccctgg cggcagggca cggttcctac
     1081 gtgagcgtct caaccagtgc ggaggtcccc aggtgactac gacccagatc ctcgcgactg
     1141 cgacccagcc gggtgccggg ggcgtggccg ccgacttctc ccaggagacc tggtggctca
     1201 ccctcatcaa ggcagtcttc gtcgtcgcct tcctcatcgt cagcgtcctc atggtcttgt
     1261 gggtggagcg gcgcgggctc ggccggatgc agacccggct gggtcctaac gtcaacggcc
     1321 ccttcgggct gctccaggcg gtcgctgacg cgggcaagct gatcctgaag gaggacttct
     1381 ggctgcgggg ggctgagagg gccgtctacc tcctggcccc cgtcatcgcc gcgctcagtg
     1441 ccttcatggt ctacgcggtc atccccttcg gacctcaggt gagcatcctc ggccacagca
     1501 cccccctgca gctgaccgac ttccccgcct ccgtcctcta catcctggct atcaccgcct
     1561 tcggggtcta cggcatcatc ctgggaggat ggtctgccca ctccacctac cccctgttcg
     1621 gggccgtgcg cagtgccgcc caggtgatct cctacgagct aagcatgagc ctgtccatcc
     1681 tcaccgtctt cctggtctcg gggacgatgt ccacctccgg catcgtctcc gcgcaggaga
     1741 gggtctggtg ggcggtagcc atgctcccca gcttcgtcat ctacgtgatc tcgatggtcg
     1801 gagaggtcaa ccgtcttccg tttgacctgc cggaggcgga gggtgagctc gtggccgggc
     1861 acatggtgga gtactcctcc atgaagttcg cctggtactt cctggcggag tacatcaaca
     1921 tgttcaacgt ctcggcggtg tgcgtcaccc tgttcctggg aggctggagg tcggtgctcc
     1981 tggccagctt ctgggagggg gccaacagcg ggtggtggcc catgctctgg ttcgttgcca
     2041 aggtgtgggt ggtcatgttc ctcatggtct ggacccgggg gaccctggtg cgcatccgct
     2101 acgaccagtt catgaggctg ggctggaagg tcctcatccc ggtgtccctg gtctggttcg
     2161 tgctcgtggc cgttgtccag gcctaccgca ccttctccgg tgcctcggtg cagtcgctgc
     2221 tgctggtgct cgctgcggtc ttcctcgtgg ccatggtggt cctcttcctc ctgcccgacg
     2281 accaggggga gcaggacgac ggggttgagg agccgcagct cctcggtgcg cgtaccgggg
     2341 acgacggcga cctggccgag gtgaccgtcc ccggggagga gcacctggcc tttgccggcg
     2401 gctacccggt gccgcccctt cccggccagc accttccgcc ctctccgcgc gcccggcgtg
     2461 gcggggagcg ggggcaggac agccacctcc cctcgcaccg atcctcccag gacgcggcgg
     2521 cactgccggc gtccaccacc aacctcgcgg ctgacgccca ggagggacct gatgactgac
     2581 cagcctaccg cctcccgcgg cacctccggt ccgcggcccc agggcggctc cgaccacgac
     2641 cagtggcggc gtgacgcccc ctccctcctg gcgcaggtct ttgcacctgt cgccgggtac
     2701 ggggtcacga tctcctccat gttccgcccc gtcgtcacgg agcagtaccc ccgtgagcct
     2761 gctcaggtca tgccccgctt ccacggacgc caccggctca accgctatga cgacggcctg
     2821 gagaagtgca tcggctgcga gctgtgcgcc tgggcctgcc cggccgacgc catctacgtg
     2881 gaggcggcct ccaacgagcc gggcgcgcag tactcgccgg gggaacgcta cgggcgcgtc
     2941 taccagatca actacctgcg ctgcatcttc tgcggcatgt gtatcgaggc ctgcccgacg
     3001 cgcgccctga ccatgtccac cgacttcgac gagcttgtcg gccccacgcg caccggcctc
     3061 atctacgaca aggacgacct tctggtcccc gtgcccgccg gtgccgtggc gccgccgcac
     3121 ccgatggtgg aggggactga ggacggcgac tactaccggg gcgcggtcac cggtcccact
     3181 caggcgcaga tcgactgggt gagacaggcc cgtccgcagg actccaccct gtccaccgcc
     3241 cgcccggtcg ccggtgacgg ccatggcagc cctggcaacg agcctgtccg ggaggccgtg
     3301 tgatgagcct gctgcccctc tccgtctcgg tcctggtggc tgccgtgccg tctgcggtct
     3361 ccacttcctc ctccacgctg cctcacaccg tcgcggctgc ggtgcccact gccctgagcg
     3421 aggctggcac cctctcagtg ggggagagca tcctgcttgc cgtggttgcc ctggtcacgg
     3481 tggcgtgcgg cctgggggtg ctcaccgcca ggcgtgccgt ggtggctgcg atcaacatga
     3541 tcggcatcat gatctccctg gccgttctct acatcgccaa cgaggcgccc ttcctgggca
     3601 tgacccaggt ggtggtctac accggggcgg tcatgaccct ggtcctgttc gtcatcatgc
     3661 tggtgggcgt gggcggggag gagcccgtgg cgggtgccgg gggctcctac cagctgcccc
     3721 tcatcggcct gctgggcctg gggctggcga ccctcctggc tgccgtcctg caccgctccc
     3781 ccctcccggc cgcggcgggc ctgcagggtg gggaggcctc cacgccggac cggatcgccg
     3841 aggtcctcct tggggaccac gtggtgacca tggagctgac gggcacgctc ctggtcgtcg
     3901 ctgccgtggg tgccttgacg ctgacgcacc gccagcgggt ccgtgcccgg cgcggccagg
     3961 cggagacggc cggggagaag atgcgggcct acgccgctac cggggcgcac cccgggcaga
     4021 agccggtccc gggcgtctac gcctcgacca acaccgtggc cgccccggcg ctgggcgctg
     4081 acggccacga ggtggctgag tccgtgcccc gcgtcctgcg cgcccgtggt cagggcatgg
     4141 agctgtccga ggtctctccc gagatggggg cggcccagcg cgcgggagag gtccttgagc
     4201 gccaggccgg ctcccggtcc acggggacgg acgctggaga cggctccgtc cacggctcgg
     4261 cggtgggccg gtccgggatg ccctccatgc ccggccaggc cgcgcccgtc gtcgaccagc
     4321 cggtggcacc ctcatccgcg ctgcgtgaca cctcgcagag cccggcactt gggcaggggg
     4381 cggccgaggg cgcggagggc actgaggagg acaccgagga gaaggagaag ggggaccagt
     4441 gactcttcct atcgctgcct acatcgtcct ggcctgcgtc ctgttcaccc tgggcgcgct
     4501 gaccgtgctg ctgcggcgca acgcgatcat cgagctcatg ggcgtggagc tcatgctcaa
     4561 cgccgtcaac ctcgtcctgg tgaccttctc ccgtcttcac ggcgacctca ccggccaggt
     4621 cttcgccttc ttcgtcatgg tcgtggctgc ggcggaggtc gtggtgggcc tgagcatcgt
     4681 cgtgtccatc ttccgtaccc gcaggtccac gtcggttgac gacgtcaacc tgctcaagag
     4741 ctgacagggc tgagaggact gacatgactg ttgcgaccgg tatcgcctct gcggtgtccg
     4801 tggctgcctc cgtccccgtg tccccccgag ccctcacccc ggcctccccg gtgctggcag
     4861 cggacacggt ggccgtggcc cagccggcca ccggcctggc cgggctgtcc tggctccttg
     4921 tcgctgtgcc ggcggccagc gctgcggtgc tcctgctggc ggggcggcgc agcgactcct
     4981 ggggccactg gctgggcctg ctggcctcac tggcgtctgc ctgcctggga ctgggggtcc
     5041 tggcccagat cctggccctg cccccccagg agcgtgtcct cagcgtgagc ctgtggcact
     5101 ggttctcctc cgcagacctg tctgtggacg tcgggctgcg ggtggacccc ctgtccctga
     5161 cctttgtggt gctggtgacc tgtgtgggct tcctgatcca cctctactct gtggcctaca
     5221 tgggtcacga ccgcgaccgg cgccggttct tcgcctacct caacctgttc gtcgccgcga
     5281 tgctgacact ggtgctcggc gacagctaca tcgtcgtgtt cgtcggctgg gagggtgtgg
     5341 gcctggcctc ctacctgctc atcggcttct ggaacaccgc agaggcggac gccccccgcg
     5401 cccagcggga gaaggccgtg gacaacgccg ttgccgcaaa gaaggccttc gtcatgaacc
     5461 gtgtcggtga cctgggcctg ctgctggcca tgatggccct ggtgtcacag gtgggctcgg
     5521 tcgccttcga caccgtcctg cccgctgcgg cccgtgggga ggtctcctcc gggtggctga
     5581 cggcgatcgg cctgttcctc ctcctggccg cctgcggcaa gtcggcccag ttcccgctgc
     5641 aggcctggct gggggacgcc atggccggcc ctacgccggt ctccgccctc atccacgctg
     5701 ccaccatggt caccgccggg gtctacctca tggttcgctc cggaccggtc ctggaggggg
     5761 cgccgcaggt acaggtggcc gtagccgctg tcggcgccct caccctggtg ctgggtgccg
     5821 tcatcggctg cgccaaggac gacatgaaga aggtcctggc ggcctccacg atgagccaga
     5881 tcggctacat gatgctgggg gcaggcctgg ggcccgtcgg ctacgccgtc gcggtcttcc
     5941 acctgctgac ccacggcttc ttcaaggcgc agctcttcct gggtgccggc agcgtcatgc
     6001 acgccatggg cgaccaggtg gacatgcggc gctttggcgc cttgtaccgg gtcatgcggg
     6061 tcacctgggt gaccatgggc atcggctggc tcgccatcct gggggttcct cccttctcag
     6121 gcttctggtc caaggacagg atcattgagg ccgcctttac cggggagggc gctcagccct
     6181 gggtcctggg gacggtcgcg ctgctgggcg cgggcctgac cgccttctac atgtcccgcc
     6241 tcttcttcat gatcttccac ggcaaggccc ggtggaccac gagcagcgac ctggagggag
     6301 aggtccaccc acacgagtcc gggtggctca tgaccctccc gctcatcgtc ctgtcgctct
     6361 tctccctggg cctgggaggg ctgctcagcc agggggaggc cttcaccacc tggctggaac
     6421 cggtcaccgg gcatgtggac cactcggccc acgagccggt cctgcccgtg acggtcatca
     6481 tgggaggcac tctggcgctg gtgctcctgg gtgcgctggc tgcctgggcc atgtacgcgc
     6541 gccgtgaggt gcccaccgtg gtccagccgg gcagcgtcat ggtggaggcg gcacgccacg
     6601 acatgtacca ggacgccctc aacgaggctg tggcgatgcg cccgggccag gggctcgtcg
     6661 cggccacgcg ggccgccgag cgctacgtcg tcgacggcgc cgtggagggg gccgccgtca
     6721 ccgcctcagc cgcaggaagc cttctccggc gcaccgagtc cgggtacgtg cgctcctacg
     6781 ccgggtacat gctgggcggg accgtccttg ccctggccgc cgtcctggca agcaggctct
     6841 gagaggacac cgccacatgc agactcttcc cgcaacgctg cccgtcctga ccatcatggc
     6901 ggtcctgccc ctggcaggcg ctgtcctcct gtgggtcctg ccgcccctgc gccgctacgc
     6961 cagcctcctg ggcctggccg tgtccctggc tgtgctcgcg gtgggggtgt gggcgctgag
     7021 catgttcgac ctgtcccagg cggggacggt ccagctggcg cagaccaccc cttggattcc
     7081 ctccttcggt gcctactggg ctcttggcgt caacggcctg ggcctgtcca tgcttcttct
     7141 gggcgccgcc ctggtgccgc tcgtgctgct ggcctcctgg aaggaggtcc cccaggagcg
     7201 ccaggccctg ctcaccggcc tggtcctggt cctggaggcc ttcgtggtgg ttatcttcag
     7261 cgcccgcgac ctgttcctgt tctacgtctg cttcgaggcg atgctgatac cggtctactt
     7321 cctcgtcggc tgcctgggcg ggccggggcg tcagcgcgca gccctgaagt tcctcctgta
     7381 ctccctggca ggggggctgg tcatgctcct gggcgtggtc atgctggcga tccacacctc
     7441 cgagaaccag gtcggcttcc tcatcgacac catggtgggc aacctgaccc tgtccccgac
     7501 catgtcccga tggcttttcc ttagcttctt tatcgccttc gccgtcaagg cacccatggt
     7561 tcccctgcac acctggctgc ctgacaccgc cgagcaggcc acacccggca cctcggtcct
     7621 cctcatcggc gtgctggaca agatcggcac ctacgggatg gtcgccctgg tcctgccgct
     7681 gttccccgag gcctcccgct gggcctcccc ggttgtggtg gtgctggccc tggtctcgat
     7741 catctacggc ggcctggcgg cgatcgccca ggacaacctc taccgcctca tcgcctacac
     7801 ctctgtcagc cacttcgggt tcatggtgct gggcatcttc agtgggagcc aggtagccgc
     7861 caccggggcc atggtctaca tggtcgccca cggcctgagc atcgccggcc tctacctggt
     7921 gaccggcttc gtcgcccggc gcaccgggac cgtgagcatc tccgagctcg gcgggctggc
     7981 tcgtgtcatg ccgctggcgg cagggacctt cctgctctcc gggctggcct cgatcgccct
     8041 gccgggactg tccggcttcg tcccggagtg gatggtgctg accgggacct tctcggtgtc
     8101 agtgcccctg ggcgtggcag cggtggccgg tgtcgtcatc gcggccgtct acgtcctgct
     8161 gccctaccag cgggtcttca ccggcgcccc ctccccggac aggacgggac taggggacct
     8221 ggagggccgt gagaggctgg tcctggtccc ggtcgtgggg gccatgctgg ccctgggcct
     8281 ggtcccggca ccgctgacca gcctcctcga cgacgtcggg gggcaggcca gcacggctgt
     8341 gagccagtcg acggcgcaga ccacggcctc tgcggggcac cagcccctgc acgcacacgc
     8401 accgctgccc gagccacgga agggagcctg acgtgaccgt caccggtcct gtaatcggct
     8461 ggggagcctt gaccccggtc ctcatcgtcc ttggcggcgg ggtcctgggg gtcctggtgg
     8521 aggcattcgt gccgcgccgc gcccgcctgg ccacccagac cctgctcagc gtggcggctg
     8581 tcctggcctc cggggcagcc ctgacgctgc gcttcgagca gatggagctc caaggtccct
     8641 cccgtaccgc gagcggcctc cttgaggacc cctttgccct gtccagccag gcggtcctgc
     8701 tggtcatcgg gttcctggcc gtgctggtca tggctgaccg caccgaggtc ggtgacggcg
     8761 ccttcgccgc ccaggccgca gaccgtcccg gcagcgccga ggagtcggag tccctgcacg
     8821 cgggctggac cacgactgag gtcttccccc tggccctgct ctcgctgggc gggatgatgc
     8881 tcttcggggc cagcagtgac ctcatcaccc tgttcgtcgt cctggagatg gtctccctgc
     8941 cgctgtacat catggccgcc acggcccggc accgccgcct gctgagccag gaggcggcac
     9001 tgaagtactt tgtcctaggt gccttcgcct ccgccttcct gctgctgggg gccgccttcc
     9061 tctatgggct gtccggcgcc gtggactacg agacgatcgg cctggccgcc cagggcgagg
     9121 cgggtgcgca ggacggctgg ctgctcctgg ccgggctggt cctggtctgc gtgggcctgc
     9181 tgttcaagat tgccgccgtg cccttccacg cctggagccc cgacgtctac cagggtgccc
     9241 cgacgccggt caccggcttc atggccgccg gggtcaaggc ggcggccttc ttcgcgctgc
     9301 tgcgcttctt ctacacggtg gtggggccgc tgggcgcaca gctggctccc ttcctatggg
     9361 cagtcgcgac agccacgatg gtcctgggga ccgtggtcgg tgtcgtccag aaggacgtca
     9421 agcgcatgct cgcctactcg gccgtcgccc acgccgggtt catgctgatc gccatcatca
     9481 ccgacaaccc tctggttgtc gcgccgctga ccttctacgc cctgacctac ggggtgtcca
     9541 ctgtgggtgc cttcggcctc atctccctcg tgcgcacgcg ccacgacggc gcggtgggtg
     9601 ccgaggccag cgacctggac tccttcaggg gacttgcccg gcgcagcccg tgggctgccg
     9661 gggcgatgac ggtcttcctc ctgtccttcg ccgggattcc cctcaccgcc gggttcatgg
     9721 ccaagttcta cctcttcgcc gcaggtgtcc tgggcggggg cacctggctg gtcgtcgtgg
     9781 ctgtggcctg ctcagctgtc tcggccttct tctacgtgcg tcttgtcgtg ctcatgttct
     9841 tccacgcgcc ccgggcagag gacgggcagg acacggtcgt ggtggacagt caggggatgg
     9901 tgaccacggc ggtgaccgtg tccgtgctag tcacggtact gctgggcgtg ttcccacagg
     9961 cagcgttcgg actcttcgcc tagacggtta tgctcctgcc gtgatcggat cgatgccctt
    10021 gagcgccccc cagttggaag ggcgcatcac ccctgccctg gaggcggttg agacccgcct
    10081 cctggaggtt gtcaccagtg ctgacgagac gatcaacccg ccgacctccc acctggcgga
    10141 ggcggggggc aagcgtctgc gaccggtact gactctcctg accgcacagc tgggggaccc
    10201 tgggctggcg gcgggcgacg aggtccgcga cgccggcgtc gccgtggagc tgacccatat
    10261 cgccaccctc taccacgacg acgtcatgga cgaggcgccg ctgcggcggg gcgcccccag
    10321 cgcccagaca gtgtggggca actccgccgc gatcctgacc ggggacgtgc tggtggcgcg
    10381 cgcctcccag ctcgtggccg gcctggggcc gcgggcggtg cttgcccacg ccaagacctt
    10441 tgagcgcctg tgcatgggac agctccacga gaccctgccc cgccctgccg gtaccgaccc
    10501 ggtggagcac tacgtccagg tcctggccga caagaccggc tccctcatcg cggtctccgc
    10561 ccgctacggt gccatgctca cccgcgccgg caggcgcacc gagcacgtgg tggaggcctt
    10621 cggagagaag atcggggtgg ccttccagct ggctgacgac gtcatcgaca tcatcagcga
    10681 ctcagacacg accggcaaga ccccgggcac ggacctgcgc gagggcgtgg acacgatgcc
    10741 cgtgctgctg ctgcgccagg ccctggccgc cggtgagctc gacgccgccg ggcagtcgat
    10801 cctgtccacg ttgtccagcg ccgacctggg tgacgacgcg gtgctggccg atgtcgtcac
    10861 gcgtctgcgc agccacccgg tccttgagcg gacacggagg atggcgatgg agtgggccag
    10921 ggacgcggtg gaggtcctgg gcgggctgca cgacgccgtc ctggagggca ccaggcagag
    10981 actggctgcc gaccaggtca ccgaccccgg tgccgtggag gctgccgtgg ctgacgcccg
    11041 ggagcgggtc agccacgtgc gtgacggcat ggagcagttc gcccggctcc tggtggaccg
    11101 agcggcctag cagcctggtc gccaccgctg gcgccaccgt cggcaagcct cgtcagcacg
    11161 accgtcagca ccgctgcccg gtcctgccgc ctggtctcgt cctgatgacc cggcggcctg
    11221 cgcgactctg gcactcgtcg agccgggctg cgggccgtgt gacgggaacc tgcccgggta
    11281 aggaggtcct gggctgcggt cagtcgctac actagggagg cctccgagcc gtctcgcggt
    11341 gccgccctcg cgacatgttc ctgcgacgtg tccccacggg cactgacctg ccacaaggac
    11401 aggaaagaca ggactgtgcg ccggccgcct gccccgggac ggtgaggagc ctggccccgt
    11461 ccccatctga caggaaccga cattggcgac accgacgaca ctgactccac gtccgctctc
    11521 cgttgccgtt atcggcgctg gccctgccgg catctacgcc tcagacattc ttgccaagtc
    11581 cgggctggac gtgtccatcg acctgttcga gcgcctccct gcaccctacg gcctcgtgcg
    11641 ctatggggtc gcccccgacc acccccggat caagcagatc atcgtggcgc tctacaagat
    11701 cctccaacgt ggcgacatcc gcctggtcgg caacgtggag gtcggcaggg acgtctccgt
    11761 ggccgagctc cacgagcact acgacgccct ggtcttctcc accggggccg ataccgacgc
    11821 ccccctggac atcccgggtg cgcaggctcc cgagtgccac gggggcgcgg acttcgtctc
    11881 ctggtacgac gggcaccctg accacccccg gacctgggac ctcagtgcca gggaggtcgc
    11941 ggtcatcggg gtgggtaacg tcgccctgga catctcccgg gtgctggcca agcacccggc
    12001 agacctcatg accaccgaga tccctgcgaa cgtggctgag gtcctcagga aggcaccgac
    12061 cacggacgtc cacgtctttg ggcgccgtgg cccggctcag gtcaagttca cccccctgga
    12121 gctgagggag ctgggtaagc agcccggcgt cgatgtcctc gtcgatgagg aggacttcga
    12181 gtacgacgag gcctcccggg ccgccctggc cgcctccaac cagcagcggc aggtggtcaa
    12241 ggcgctggag ggctatgcga tgcaggagcc tgaggacctc accgctcccc accggatcca
    12301 catccacctc ttccaggcgc ccaccgaggt cctcaccgac gacgccgggc acgtgaccgg
    12361 gctgcgtacc gagcgcaccc gcctggccgg ggacggcagt gtcgtgggaa ccggtgagct
    12421 ccgtgactgg ccggtccagg cggtgtaccg ggccgtaggc tacgcgggct ccccggtgga
    12481 ggggctgcct ttcgaccacg agcgccatgt gctgcccaac gagggcgggc gtgtcatcgg
    12541 ggaggacggc cggccgatga ccggcgtcta cgccaccggg tggatccgtc gtgggcctgt
    12601 gggccttatc ggctccacca agtctgacgc ccaggagact gtcgccaacc tggtggctga
    12661 cgcccgggcc gggcttctcc acgcgaccgc gcaggaggac tcccaggtcg gccacgctgc
    12721 cttgatcgcc ctcctggagg aacgtggtgt gccctacacc acgtgggagg ggtgggagct
    12781 gctggacgcc tacgagcgcg agcttggcag cgcctggggt gaggttgagg gcggccgggg
    12841 cctgcgcgaa cgcgtcaagg tggtctcccg ggacgccatg accgcggtct cgcgctccac
    12901 ggaggtccct gacgacctga tcggccagcc ggaggccgac cgcgtgcctg agcgcgtcgc
    12961 ccaccgtctc cgggacgccg tagaatcagt gtcctgaccg tgtgtcccag cctgctggct
    13021 gtcagcgcac ggccagggcg tcgtcgtcca gccgtaccgg gccgtcaggg gctggttgtc
    13081 ggctaccacc cccacggaag gcaggtccat gcgagtcggt atccccactg aggtcaagga
    13141 cagcgagttt cgcgtcgcga tcaccccggc cggcgtccac gcgctggtcc agcgcggtca
    13201 tgaggtccgt gtccaggtcg gcgcgggcct gggatcggcg atcacggacc gggagtacgc
    13261 cgcagcggga gcggtcctgg tggagggggc cgcgccggtg tgggcggagt cggagctggt
    13321 tctcaaggtc aaggagccgg tggcgtgcga gtacgagcac atgcgtcccg ggcaggtgct
    13381 cttcacctac ctccacctgg ccgctgaccg gcggctgacc gaggagctcc tgtctcgtgg
    13441 ggtcacctcc gtcgcctatg agacggtcga ggctgacgac ggctccctgc cgctgctggc
    13501 ccccatgtcc gagatcgccg ggcgcctggc tgcccaggtt ggtgcgaccc agctgctgcg
    13561 gcccagcggt gggtccggag tgctcctggg cggcgcctcg ggggtacgcc gggggcgggt
    13621 ggcggtcctg ggaggcggga ccgctggcct gtgcgcggcc caggtcagtc tcggcatggg
    13681 tgctgacgtc actgtcttcg acatcagccc ggccaggatg cgccacattg aggaggtctc
    13741 cggcaggcag atccgtaccc gcttctccac ggagctcgat gtcgcccagg cctgtgccca
    13801 ggcggacctt gtcatcggcg ccgccctggt gcccggggcg cgggcgcccc acctggtgac
    13861 ccgtgagatg gtgactgcta tgcgcccggg cagcgtcctg gtggacgtcg ccatcgacca
    13921 gggagggtgc tttgaggact cccgcccgac cacgcacgcc gcgcccacct ttgaggtggg
    13981 gcagaccacc ttctactgcg tggccaacat gcctggagcc gtcccctaca cctctaccta
    14041 cgcgctgacc aacgccaccc tgccctatgt caccgtcctg gcggaccggg gctggcggga
    14101 ggcctgcacg gcccggccgg acctggcccg gggcctgacc acccacgagg gcgtcctcta
    14161 cacccgtggc gtgggtgagg cgctggggat ggacaccggc tccgtggccg acctgctcgg
    14221 ctgaccggct gcccgaggtg tcgggcgcgg acgggcccgg cccggcacca cctagcccag
    14281 taccacctag gtgtacttgg ctgtgaggtt gtgtacgtgt tgtgcgggtg tctggctgtc
    14341 ggtgctggtg tggggttggt ggtggttgta gtggtggagc cagtcgttgt aggcggcggc
    14401 tggctcggcc tcgctggtgt ggggccttgc gtgggctggc tcctcgttgc ggggtggggt
    14461 tgaggtgctt gaccttggcg cctgtctggg gacggtaggg gcgggtgcag cggtgcctgg
    14521 ccccggtggt cccggcgacc cgggtgctgg cggggtcggc ccctggtggt ggcgcggccc
    14581 ccggccgcag cggtggtggt gaccaagccc ctggccacgg tggcgggagt gggctgccgt
    14641 gtctgcctga ggtcgtggtg gtggggagtg tcctgtctgg gggtgcctgg ggtgggtctt
    14701 gtgtctgggg gttgtggtgg tggttacggt tgggttatgc ggatgagcaa ggaggagtgg
    14761 cgccaggggt ggctgcacgt caccccggag tgggtggacg cgaacatgga cgacctggtg
    14821 aaggaggggt acgcgccgat gggtgtgggt gccccggcca cggccgagca cctgggctac
    14881 tttggtgggg tgcttccggc ctcggtggtg cacctgtggg agcggttcgg gttcgagggc
    14941 ttcgggcagg ggcggtggtg gttcaccgac cccttccagt gggcgccggt ggtggaggcc
    15001 tggctggagg gcacccctgt gccgttcccc agccagcggt ggtggtgcct ggcccgcaac
    15061 gccacgggcg acatgaggct gtgggggagg tctccggccc cgccctggac gtcgacccag
    15121 tgatggggtg ggtgcgtccc gacgcgggcc acgcccggga catggctgac tcggtgatgc
    15181 gtgagcgcat gggtgccatt gccatgacct ccccccgcag ggagagcctt gatgatgacg
    15241 acaccggggt tctggtggct gactgggcca tcgagaacct gggggtgctg gaggcggggc
    15301 aggtctacgg gctgaccccg gcctactgcc tgaccgggag ggtcagcctg gaccaggtcg
    15361 gtatcgagga cgccaccgcg cacctcactt ttctggccca ggcccaggag cacaccctgg
    15421 tggaggactt ctccgccgcc gccgcacagg tcgccgccag catcgccgca ggaggccaga
    15481 ccaccagcca gggtgacggt cccggcggca ccagtagcgg ccctggtggc accggtcccg
    15541 gtccgggggc tgggtcgtga cgcccgggcg ctggcggggc tggctgcgcg ggcgcgggcg
    15601 ccccaccgcc tctgctgctg gtcccgccgc gcctggcggc ggggccggtg gtggacaggc
    15661 ccccggcccc ggccacagca gcggcggcgg cgatggcgcg gccctggtgg ctggtgacat
    15721 cttcaccccc ggcagggccg gcgggcaggg ggtgctggtg tgggaggggg cggtggcagc
    15781 ccctgacagc ccgctggcgt ggcactcggc caccccggtg gaggtgttct tccacaccgg
    15841 ccaccaccgc gccacccagg agctggcctc cgagttcacc cgccagctgg accgacaact
    15901 ctccgccctg tccctgctca gcgctgagcg gctgctggag ggcatccgcg cctaccgcac
    15961 ccagggacgc cagcccacct ccaaggccac caggcgagcc cgtgacacct tcatggacaa
    16021 ggtcgtggac gacctgcggc gtgtgcacca gctctcccgg gaggacgcgc gcgccagggc
    16081 ggctgaggtc gaccgggcgc tggtggtgct ccacgagccc gaccagctcc tggcggggcc
    16141 aggcggcccc gccaccaccg ggccgcagcc cggctacccc tccctgggcc acgggcaggt
    16201 caactcctcg gtgggctccc agaacaagcc catggtggcg gtcctggagc aggccgccct
    16261 ccaggtcccc cccggagcag cgttcccggg cgcgcctgct ggtgcgcgcg gtgctgacag
    16321 actcccccga cctggcccag gacctgcgcc acggccaggt ggtcctggag ccccgcaccc
    16381 aggcccaggt gagcgccacc agcccccggg ggccaccgtg gacacccagc cacgtcgccg
    16441 ccaccatcgc caccggccac gacccctggc acccaccacc cgcgcaggag cagagacacg
    16501 gccagacccg ccaggacacc cggggccaac agcccggcca ggaccagccc ggggcaccca
    16561 ccggggccga cccctcccgc accaccagcc tgcgcgaggc ctccttcccc cacccgccta
    16621 ccgccgccgt gcgcgtacca ccaccccaga cgggccagcc gtcgcggccg ctgacccgcg
    16681 aggaggtcct ggccaggatc accacccagc agccccgcac cccacgccag acccccacca
    16741 gaaaacaccg acacaaccga ggcctgggcc gctagcagcc ggtgccagcc gcgtgagggg
    16801 ctggcagcct tcctggtgac gttcctggcg gcccgcgccc acggacgcgg gccgcagcgt
    16861 ggtcctgggt ccgcccgcca ccgccccacc caccacctgg cccggtccgg cgtgaccggg
    16921 cacgaccccg gcatactgtc ggggtactgt ggcgccccgc ccgcgtcctt gtgccccgtg
    16981 cccaggaggc ccccggggcg cccgaccaca tccgcacggc ccaggagacc ttgatgacga
    17041 caccaatcgg ctctctcgtc cccacgcact ggtacaacct cgcggcggac ttccccgagc
    17101 ccctgccacc ggccctgcac ccggggacgc gcgagccgct gcgccccgag gaccttgagc
    17161 acctgttccc ccgcgagctc atcgcccagg agctctccac cgagcgcttc atcgagattc
    17221 cccaggcggt gcgggaggtc tacgccaagt ggcgtccctc cccgctcatc cgtgccgatc
    17281 gcctggagcg ggtgctgggt acccgggcca ggatcttcta caagtacgag ggggtcagcc
    17341 cggctggctc gcacaagccc aacaccgctg tcgcccaggc ctggtacaac gcccaggagg
    17401 ggaccacccg gctgaccacg gagaccgggg ccgggcagtg gggcgcctcg ctggctctgg
    17461 cctgctccct gttcgggatg agctgcgagg tgtggcaggt gcgttcctcc tacgagtcca
    17521 agccctaccg gcgctaccag atggaggtct acggctcgac ctgccactcc tcaccctctg
    17581 agctgacccg ggcgggcagg gacgtgctgg cccaggaccc gcagaccacc ggcagcctgg
    17641 gcatggcgat cagtgaggcg gtggaggtgg ctgcggccag tgaggacgtc cactacgccc
    17701 tggggtcggt gctcaaccac gtcatgctcc accagacggt gatcgggctg gaggccgtgg
    17761 cccagctgcg ccaggccggg gtggaccagc ccgacgtcgt ctttgggtgc gccgggggcg
    17821 gctccaacct ggcgggcctg tccttcccct ttatcgggcg caatctcact gagggcacca
    17881 gcagccgtgt cgtcgcctgc gagcctgcgg cgtgccccac gatcacccag ggggagtacc
    17941 gctacgacgt cggagacgtc gctggtacga ccccgatgat gaagatgtac accctcgggg
    18001 cggacttcgt gcctcctgcc atccacgccg ggggcctgcg ctaccacggc atggctccca
    18061 tggtctccca cgcggtccac ctgggtctca tggatgctgt cgccgtgtcc caggaggacg
    18121 ccttcgccgc aggtctgctc tttgcccgca gtgagggagt cgtcccggcc cctgagtcca
    18181 cccacgccgt ggctgccgcc ctggagcacg cccgccaggc aaggaatgat gaggtcattc
    18241 tcatcggcct gtccggcaac ggcgtccttg acctgcctgc ctacgaggcc tacctctgac
    18301 tggcctgtcc cggacgggga gccgcccaac ggccaggaca ttgttggctc acagggggtg
    18361 gtcaggaagt gcgcaccgct tcttggtagc ctgcgaccat gaacgggacg aaccattaca
    18421 acggtctcaa gaccgccgtc ctcctgggcg gtatgtgggg actgctcatt ctcatcgggt
    18481 acgtcctggc ccaggggacg ggctcgtcgg tctggctgtt cgtcatgccg cttatcggtg
    18541 tggcccagac ggcctacgcc tactggaact ctgacaagat cgcggtgcgc tccatggggg
    18601 ccattgaggt ctcggaggcc cagtacccgc agatgtacgc catcgtgcgt gagctgtcca
    18661 cctcggccgg gcagccgatg cccaggctct acgtggcgcc cacggtgagc cccaacgcct
    18721 tcgccaccgg ccgcgacccc cagcacgccg ccgtgtgctg cacccagggc atcctccagc
    18781 tcctgaacga ccgcgagctg cgcggggtcc tggggcacga gctgtcccac gtgtacaacc
    18841 gtgacatcct caccagctcg gtggcggcgg gaatcgcggg ggtcatctcc tcgatctcca
    18901 ccatggcgct gtggttcggg ggaggccgtg accgccgtga cggcggcaat gtcatcgtcg
    18961 tcctgctgct ggcgctcctg gcgccgctgg ccgctgccct gacgcagttc gccatctcgc
    19021 gcacgcgtga gttcgatgcc gaccacgacg gcgctgtgct cacccacgac cccctggccc
    19081 tggccagtgc cctgcgaaag ctggagaccg gcgtcaacgc cgccccgctg gcccaggacc
    19141 cccgggtcga gccggtgtcg tccatgatga ttgccaaccc cttcgggtcg cgggtgcgca
    19201 acctctttgc cacccacccg cccatggacc agcgcatcgc ccgcctggag aagatggctg
    19261 gctactgacg ctgaccggcc aggctgccgg gagggctggt gctggcgcac ggaggcgcgc
    19321 cgtgctgaga accgtgctga gatagggcgg tggacagcac gcaggaggtc gtggcccgca
    19381 cggactcagc cgagttcgcc tccgttctgc gccgggcggc cgagtcgggc agagtggcgg
    19441 gtgtcggtgc cggctctgtg cgactcaccc ggctaggggc gggtgagagc tttaccgcct
    19501 ggctgctgcg tccctgccac gaggatggcc gtccgctgcg ggtcgtgcgg gtgccgcgcc
    19561 tgcctgctga ggagctgccc cgttccctga gaggggagta cgaggcgctg tcacgtgtgc
    19621 cagcggacct ggggtcgcac ggggtggccc tggaggagga cagtgacaac gccctgggtg
    19681 cgccctacct ggtgactacc tacgtgccgg ggcgggtgct cccgccctgg gcgtggacgc
    19741 ctcgtctggc ctgtgccctg gcagagcagg tggcccgcct gcaccacctg ctgggggagg
    19801 ctcagacggc cggggagggg acggctggat ctcagctgca gtccggtcag ggcagtcacc
    19861 ccggtgctct gcgggaggct gaggaggtcc tggggtggtg gaggtcccac caccccgacg
    19921 tgctggagga gcctcgggcg tcgtgtctgc tcgacccgtg gtggcaggcg ctggccagcc
    19981 tcaacccggt gacggtgggg acgcccctgc acccgcttgt ccacggggac gtggtggtga
    20041 ccaacgtcgt cgtcggtcct gacggcgtcc cccggctcat tgactgggag tgggcggcgc
    20101 cgggggaccc ggctaaggac ttggcactca tcgggggaga ggtgacgggc gggccctggt
    20161 acgcccacct gtcccgggag gcggtgacgg acatggtctc ccgctacgtg acggttcgtc
    20221 gtcggctggc tgggtcggaa gatgcggaca agggcaggga ctgcgagggc gccgggggcc
    20281 gcgccgaaaa ggccgaggag catgacgagc aggtgcgccg cctgctggtg cggcgtgacg
    20341 cctgggagct ggtggaccgg gtgtccaacc tgttgtactg cctgagccgc agagggcagg
    20401 ggaactacct ccggtgggcc agggagctgg cggaaagcct ggaggctcgc ctggcggcac
    20461 tgcccgacta gcaggtggcg gtgtggactg gctacatgcc gcaccgaggt gtaccgattc
    20521 ctcacaggtg actgacgtgg ccgtcgttct gtgaggattc ggtgcacctg tgaggaatcg
    20581 gttgttatga gggggcggac gtggagccgt gacgcctgcg tgggtcgcgg gcaccacggc
    20641 ggccagaagc gccggggacg cccagggcca ccgcagccta gccaggctag ccctgcggct
    20701 cgcccaggca gaagacaaag tcgtaccggc ctgcgctgac accccagtgg gtgtagtgcg
    20761 tgaatgacca cccgtaggtg tgggcagtat ctgctgcggc ctcctggcac tcgctgaccc
    20821 cgaggcctcc catcacgggc tggttgacgt tgtggatcac ggccagcacg ggataggtgc
    20881 ccgtggtgca ggcggccttg gcggcctcgg gggactccgc gtcgatatcg tcaacagcga
    20941 ggcagtcacc gtcctttact gtgctcagcg actcctcggg atcgtcgttc ctgcctatga
    21001 ggcacaggtc ctcgtacacg gcctccgtcc caggggtgct ggcgcaactc cgcttggggt
    21061 tgtccctcct ctccgctgcg atgttggcga ccacccggta caccgcgtca
//
