LOCUS       NZ_CP025227            10930 bp    DNA     circular CON 14-APR-2024
DEFINITION  Actinomyces wuliandei strain 299 chromosome, complete genome.
ACCESSION   NZ_CP025227
VERSION     NZ_CP025227.1
KEYWORDS    RefSeq.
SOURCE      Actinomyces wuliandei
  ORGANISM  Actinomyces wuliandei
            Bacteria; Actinomycetota; Actinomycetes; Actinomycetales;
            Actinomycetaceae; Actinomyces.
REFERENCE   1  (bases 1 to 3107761)
  AUTHORS   Zhu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2017) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No.155 ChangBai Road, Changping District, beijing
            102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. HGAP 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004010955.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 19:14:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,606
            CDSs (total)                      :: 2,544
            Genes (coding)                    :: 2,496
            CDSs (with protein)               :: 2,496
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 18 of 48
            Pseudo Genes (incomplete)         :: 37 of 48
            Pseudo Genes (internal stop)      :: 3 of 48
            Pseudo Genes (multiple problems)  :: 9 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:15:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 60845
            Orig. end                         :: 71775
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP025227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10930
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10930
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10930
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5930](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5930)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(910..925)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.48"
                     /tool="antismash"
     gene            1065..1568
                     /locus_tag="CWS50_RS00270"
     CDS             1065..1568
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CWS50_RS00270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="WP_206610424.1"
                     /transl_table=11
                     /translation="MTATSSRRRGPTWPSATPTSRVSGWLPAPGTDVEGGNRCQRSTSA
                     YAHLVSQCTGTELVFRACSGARTGDFYQDNTAWDEPTQLGALSADTDLVTFSVGGNDTG
                     FAEVLGECLSRAQQGDRDPCSTDEQLVSRVGSAIDALRGTGVREGTCSLPCVTDLPSGR
                     PEGR"
     gene            complement(1736..2671)
                     /locus_tag="CWS50_RS00275"
     CDS             complement(1736..2671)
                     /EC_number="2.3.1.39"
                     /GO_function="GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_AT (9-294). E-value: 3.4e-45. Score:
                     146.6. Matches aSDomain:
                     nrpspksdomains_CWS50_RS00275_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="CWS50_RS00275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ACP S-malonyltransferase"
                     /protein_id="WP_127841186.1"
                     /sec_met_domain="PKS_AT (E-value: 1.5e-43, bitscore: 146.6,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYDYIVAAPGQGSQKPGVLDGWMDRSRTARRQVEEWSSRCGVNL
                     IEASRDDEKLKDTAFAQPVIVAASLLSVRQTVRYLGASSEAILFAGHSVGELAAAAAAG
                     YITDADAVSLACVRGAAMSAACTEPRTGMAAVMPTRRDPASDEEIQQTIHDAGLEIANW
                     NGCHQYVAAGRYERITALAEQEHEGVRVVTLPVAGAFHTSTMEPVVTVFEKALSDTEFR
                     DAGTALVGNGDGEVVTTAADVRSRLLAQVTSPVRWDLCMRTLNKQAKDATFIELAPRGP
                     LTRLYRGTYPDTTCVPVSRPEDLAVFNMLG"
     aSDomain        complement(1790..2644)
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CWS50_RS00275_PKS_AT.1"
                     /evalue="3.40E-45"
                     /label="CWS50_RS00275_PKS_AT.1"
                     /locus_tag="CWS50_RS00275"
                     /protein_end="294"
                     /protein_start="9"
                     /score="146.6"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="PGQGSQKPGVLDGWMDRSRTARRQVEEWSSRCGVNLIEASRDDEK
                     LKDTAFAQPVIVAASLLSVRQTVRYLGASSEAILFAGHSVGELAAAAAAGYITDADAVS
                     LACVRGAAMSAACTEPRTGMAAVMPTRRDPASDEEIQQTIHDAGLEIANWNGCHQYVAA
                     GRYERITALAEQEHEGVRVVTLPVAGAFHTSTMEPVVTVFEKALSDTEFRDAGTALVGN
                     GDGEVVTTAADVRSRLLAQVTSPVRWDLCMRTLNKQAKDATFIELAPRGPLTRLYRGTY
                     PDTT"
     CDS_motif       complement(2306..2404)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CWS50_RS00275_0001"
                     /evalue="3.00E-10"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="CWS50_RS00275"
                     /protein_end="122"
                     /protein_start="89"
                     /score="32.1"
                     /tool="antismash"
                     /translation="AGHSVGELAAAAAAGYITDADAVSLACVRGAAM"
     gene            complement(2710..3165)
                     /locus_tag="CWS50_RS00280"
     CDS             complement(2710..3165)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CWS50_RS00280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_127841187.1"
                     /transl_table=11
                     /translation="MTHGVPDPGHLLPYGYPALHVDRVTGYEAGRLTAVKAVSSHELDA
                     MAGGSSRPAQLPASLMIESFLQACGILLKHEAEGDQETLVVFAAARGMTVQDLPRAGEL
                     VVHTVECLDRDGDVAEFTGSSATSEGRPLMSVTRVTALQRPAASLGQ"
     gene            complement(3162..3605)
                     /locus_tag="CWS50_RS00285"
     CDS             complement(3162..3605)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /locus_tag="CWS50_RS00285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_127843112.1"
                     /transl_table=11
                     /translation="MTAPETLAPGTPAPGTPGTLSVRPDARRLPLVALDRCEVEGEPVR
                     SAKTVRTDDPYLAGHFPSLTVYPGVFLLEGVQQTLETALDGALGGVELVEVRSIRISRP
                     AFAGQTVDFDTTVTHDGDLVLTSTSCTVAGRRTASIKASWRRR"
     gene            complement(3608..4339)
                     /locus_tag="CWS50_RS00290"
     CDS             complement(3608..4339)
                     /NRPS_PKS="Domain: PKS_KR (12-162). E-value: 1.1e-17.
                     Score: 56.5. Matches aSDomain:
                     nrpspksdomains_CWS50_RS00290_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /locus_tag="CWS50_RS00290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_127841188.1"
                     /sec_met_domain="adh_short (E-value: 6.8e-35, bitscore:
                     117.9, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEESAGPARSAFVTGASRGLGAAIARDLAAQGYRVVGTARNPAE
                     DVVDGVSFVACDVTDPGSIRSAVAQARDLVGTIDIVVTSAGVMHDGPLVSLPQEAVDDV
                     ITTNLLGSVNVARETLGDMMMNQWGRLVFVGSVVAMWGSPGQVNYGASKGGLVGAARSL
                     AWELGRFGVTVNVILPGLIDTGMAANVSAKRHKELMAGTPMRRPGRPEEVTSLVSYLVS
                     EGAGYITGASIPVSGGAGMGL"
     aSDomain        complement(3854..4303)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CWS50_RS00290_PKS_KR.1"
                     /evalue="1.10E-17"
                     /label="CWS50_RS00290_PKS_KR.1"
                     /locus_tag="CWS50_RS00290"
                     /protein_end="162"
                     /protein_start="12"
                     /score="56.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="FVTGASRGLGAAIARDLAAQGYRVVGTARNPAEDVVDGVSFVACD
                     VTDPGSIRSAVAQARDLVGTIDIVVTSAGVMHDGPLVSLPQEAVDDVITTNLLGSVNVA
                     RETLGDMMMNQWGRLVFVGSVVAMWGSPGQVNYGASKGGLVGAARS"
     CDS_motif       complement(3887..3946)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CWS50_RS00290_0002"
                     /evalue="2.60E-06"
                     /label="PKSI-KR_m4"
                     /locus_tag="CWS50_RS00290"
                     /protein_end="151"
                     /protein_start="131"
                     /score="20.0"
                     /tool="antismash"
                     /translation="VFVGSVVAMWGSPGQVNYGA"
     CDS_motif       complement(4238..4300)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CWS50_RS00290_0001"
                     /evalue="2.70E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="CWS50_RS00290"
                     /protein_end="34"
                     /protein_start="13"
                     /score="16.8"
                     /tool="antismash"
                     /translation="VTGASRGLGAAIARDLAAQGY"
     gene            complement(4336..5004)
                     /locus_tag="CWS50_RS00295"
     CDS             complement(4336..5004)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /locus_tag="CWS50_RS00295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD-IA family hydrolase"
                     /protein_id="WP_127841189.1"
                     /sec_met_domain="HAD_2 (E-value: 1e-07, bitscore: 29.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLAIWTDFRGVLTVPLSEGFARYCQDKPFTPRQLGTCLAALASR
                     HGCPDGMAVLDSGVLTEREWLDEISGLLSQQFGITGSVSGMADEWWSDRRLNHQWLRFL
                     EERRQAGDFVAVMSNLPPEWQGRFEEFLPWHDLFDAVVVSCEVGARKPEPGIFREAERV
                     SSLPAGSSVLVDDITANVQGARDAGWNAVVGGGDATDDSIAYIEELASLHSGLQEGQHS
                     "
     gene            complement(5001..5930)
                     /locus_tag="CWS50_RS00300"
     CDS             complement(5001..5930)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /locus_tag="CWS50_RS00300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_127841190.1"
                     /sec_met_domain="AfsA (E-value: 4.3e-12, bitscore: 43.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPQAATVAPRNLVHRQHEQEVLVSAPTRLEDGLYESAPGPGEPS
                     PYYRDHPHSYWADVLFLAELGRQAGMSAGHAYAGLSLGTAFFFNSVMVEILDLPRLNGL
                     RDRPRVRTRQEVLATRKDGTPRQLVFSQEIIDPADAPLARTRMEVQGLQAAKYEEIRRY
                     QRGGSQPPRTSTVEVQAVSGTHVLPSSLLGREDMSNVVVSDLDCGPGRSRARLSPDFAN
                     TSLFDHDYDHYPAMVLIEAGRQVTVGSASGGNGFPVVTSVSARFPGFAELDAVTTVSAQ
                     ECANRFEVSVEQNGSAVSSMQMAVEWLR"
     misc_feature    5966..5978
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.33"
                     /tool="antismash"
     gene            complement(6017..7093)
                     /locus_tag="CWS50_RS00305"
     CDS             complement(6017..7093)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /locus_tag="CWS50_RS00305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_127841191.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 4e-10, bitscore:
                     36.9, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGAPEMQISECAVLTAAGNSMDTLTEAIDRPLGSRAPSELGALPD
                     PEVYEGVPGRVAAVPDFRIEDYVPRRGTRSLDRMTRLGIAASVLLRRQMEERRDIAADS
                     WRETGISLGTSAGSLRSNVDLTLDVIRGRGADLINPKVFPNSTMNCCASQVAIWNDFHG
                     PNATVANGTVSALSALRYASRLMSLGHCSRMLVGSVEELSPQRAWGTRLSGRIDSEVVI
                     GESATLFSLEPAGASPSPVATVTGCEVAFCPISADGARSETLRRVVDRVCSQPEDVDLV
                     VPGATGTVWQLSEERSLEHVTGRRLDVRRSVGDCGAGTSGVQVAAALTCAGPGDRVLLT
                     SMDESGVVAAATLQMCRS"
     gene            complement(7097..8338)
                     /locus_tag="CWS50_RS00310"
     CDS             complement(7097..8338)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (53-404). E-value:
                     5.7e-52. Score: 169.0. Matches aSDomain:
                     nrpspksdomains_CWS50_RS00310_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2fas"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /locus_tag="CWS50_RS00310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="WP_127841192.1"
                     /sec_met_domain="t2fas (E-value: 2.4e-109, bitscore: 363.0,
                     seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.9e-40, bitscore:
                     134.4, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRSRRVVVTGLGPVSSIGIGAEEFRALLFAGESGISPVESFDTS
                     DFQATLAGEVKNFDPADVVENINPRRWGRTAQLAAAAARLAVRDAGLDLDQETRLSTGV
                     VIGTTNGESQALDELTVQWHERGPGRMAPTVVRQIPAGNIAAAVATELQVEGDVMCLPT
                     ACSAANYAIGSAADSIATGSADVMLAGGADSVNRTTHAGFMSLGAIAKELPQPFDADRS
                     GIVTAEGGAVLVLEELSHALRREAPIYAEVLGYGLNCDAHHIVHPDATSISECIRRTLA
                     QANITPDDVDYVCAHGTGTPTNDVTEVSATRMVYGDDHPPVSSIKSMLGHTMGAASGFG
                     AIACCQAIQIQKLPPTTNLRTVDPELGEGIDFVAGSSRPARVRTALNHGFAFGGNNAIT
                     VFSEFHKDSADKEV"
     aSDomain        complement(7127..8179)
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CWS50_RS00310_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="5.70E-52"
                     /label="CWS50_RS00310_PKS_KS.1"
                     /locus_tag="CWS50_RS00310"
                     /protein_end="404"
                     /protein_start="53"
                     /score="169.0"
                     /tool="antismash"
                     /translation="EVKNFDPADVVENINPRRWGRTAQLAAAAARLAVRDAGLDLDQET
                     RLSTGVVIGTTNGESQALDELTVQWHERGPGRMAPTVVRQIPAGNIAAAVATELQVEGD
                     VMCLPTACSAANYAIGSAADSIATGSADVMLAGGADSVNRTTHAGFMSLGAIAKELPQP
                     FDADRSGIVTAEGGAVLVLEELSHALRREAPIYAEVLGYGLNCDAHHIVHPDATSISEC
                     IRRTLAQANITPDDVDYVCAHGTGTPTNDVTEVSATRMVYGDDHPPVSSIKSMLGHTMG
                     AASGFGAIACCQAIQIQKLPPTTNLRTVDPELGEGIDFVAGSSRPARVRTALNHGFAFG
                     GNNAITVFSEF"
     gene            complement(8335..8610)
                     /locus_tag="CWS50_RS00315"
     CDS             complement(8335..8610)
                     /NRPS_PKS="Domain: PP-binding (11-78). E-value: 1.9e-15.
                     Score: 48.9. Matches aSDomain:
                     nrpspksdomains_CWS50_RS00315_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /locus_tag="CWS50_RS00315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_127841193.1"
                     /sec_met_domain="PP-binding (E-value: 5.6e-14, bitscore:
                     49.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSSTSNEIRDAISEIVVEELELDIEVDELDPHADLIDEYGADSL
                     GLIQVLAQINKTLGIRVPREEAADLRTLDLLVAKTVRLQAEQGSDK"
     aSDomain        complement(8377..8577)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CWS50_RS00315_PP-binding.1"
                     /evalue="1.90E-15"
                     /label="CWS50_RS00315_PP-binding.1"
                     /locus_tag="CWS50_RS00315"
                     /protein_end="78"
                     /protein_start="11"
                     /score="48.9"
                     /tool="antismash"
                     /translation="AISEIVVEELELDIEVDELDPHADLIDEYGADSLGLIQVLAQINK
                     TLGIRVPREEAADLRTLDLLVA"
     gene            complement(8707..9870)
                     /locus_tag="CWS50_RS00320"
     CDS             complement(8707..9870)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /locus_tag="CWS50_RS00320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_164860031.1"
                     /transl_table=11
                     /translation="MNSDTLVIGGGPSGLTAGCCLVERGQSVRIIRKHPLLTSQSRATV
                     LWPKALEVLHPFGIAERLRPLGDVINSLSYYSDGRQIGTLSTRLLEGTRFDYALGISQH
                     NVETALTEVFTARGGVIEDAEVTDLTQDDDSVLLTIRSFDGTVRQERAKHCVVADGAKS
                     LGREKLGLRMEDLRDTVEFQIADVRAEGLPRDEAFYWWSQYGAIAVAPHDQTTYRLAYP
                     AVTGTPAPDAVSTQYLESLLDQRGPSNPDRHVTQLITTANFVARFAVVDQFAKGRCYLT
                     GDAAHVMAPTGAQNMNAGMLDAVCASKFIMGDIEPVDSGTPLAQLYDKMRRASIDKVFD
                     TALGHARDGAAMTPESIAERDTRYALLENPDAARARLTRMSQLDIEA"
     misc_feature    complement(9884..9897)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.63"
                     /tool="antismash"
     misc_feature    9884..9900
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 20.85"
                     /tool="antismash"
     misc_feature    complement(10645..10660)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.4"
                     /tool="antismash"
ORIGIN
        1 tctgtggttc gtggtagtgg gcggctcctg gtgggtgcgc caacggcggg ggcgttagcc
       61 aggggctgtt gtgagactcc gctgggactg ggtgcggctg ggaaccggag tcccgggcaa
      121 cagctccctc gctcggcggg ggcgtggttt tgacggcggc cttcgggaac cggaacagca
      181 atagctcccc cgctcggcgg gggcgtggcg gccgaggtcg cgaggaccgt cttctccggc
      241 tgccacgtgt cccccggcgg gggcgtggca gcctgctatg tcgtttctta catagcccgg
      301 gtggggtaga agtagtcgta tcagcgatgc tgtcccagcg ggaagggcgc aaagtgggct
      361 tgcgtgaatc aggctgagat ggagcgcgtc cgtgcgggtg gaactcctac cagcgtgcct
      421 tgaccagggt ttcgtgagcc tggctcacgc ggctccatcc tggcgggacg atatccacct
      481 gctcctgaag cgggtcgtgc aggctcgcac cgctccaccc tacgtgacag cctgacatcg
      541 acaacactct tggccctggc gggcctggcg ggcctggtgg gcctggtggc cctggcgggc
      601 ccggcggggc acacacctcg ccgcttctgg gagacgagtc tcacccgcga ggttaaggta
      661 ggtgacaggg cggtctcagg tgaaccgggc acgtgctcca cgcgtgcggt gctctgcggt
      721 gctccgtgtt gctgctccgg cttctcccgc ctcagtactc aaccacctgg caccatccgt
      781 cggccttctc tctgacacgc gagccgacag gagcaccatg accgccacga cgaccctcac
      841 gacccccgta acccccgcaa cgatccgcac atcatccggt acgtcgcccc gtgcgtaccg
      901 cctacccgcc cgcacagtca ttcgtgcggt gctgccccgc ccgctgccgg gactgctgac
      961 agtcctggcc atcttggcca tggtgcttct ccctgccgcg accgcacctg cggctggaca
     1021 gacgaccgca tctgcggccg ggcagccggt acagcccgac accagtgacg gcgacctcct
     1081 cccgccgcag ggggcctacg tggccctcgg cgactcctac gtctcgggtt tcggggtggc
     1141 tccctgcccc gggcaccgac gtcgagggcg gcaacaggtg ccagcgctcc acctcggcct
     1201 acgctcacct ggtctcccag tgcaccggga ccgagctggt cttccgcgcc tgcagcggcg
     1261 cccggaccgg ggacttctac caggacaaca ccgcgtggga cgagccgacc cagcttggtg
     1321 ccctcagtgc ggacacggac ctggtgacct tctccgtcgg cggcaacgac acggggtttg
     1381 ccgaggtgct gggcgagtgc ctcagccggg cgcagcaagg cgatcgtgac ccgtgcagca
     1441 ccgatgagca gcttgtctcc agggtcggct ccgctattga cgccctgcgg ggcacggggg
     1501 tgcgcgaggg cacctgcagc ctcccatgcg tcacggacct gccgtcggga cgtcctgagg
     1561 gcaggtgacg ggaaaccggg gaggggccga ccaggcggct ggtcggcccc tccccggcgt
     1621 gggggctgaa ggcctgtgcg cggaggctcg cgcgcggaga accttgcacg gggcctgcgc
     1681 acggaggcct gcgcacggag gactctgccc ccgcatcgct gtgccgcctc cgccctcagc
     1741 ccagcatgtt gaagacggcc aggtcctccg gccggctgac gggcacgcag gtggtgtcag
     1801 ggtaggtccc gcggtagagc cgggtcaggg ggccgcgggg tgccagctcg atgaaggtgg
     1861 cgtccttcgc ctgcttgttc agcgtgcgca tgcacaggtc ccagcgcacc ggtgaggtca
     1921 cctgcgccag caggcgcgag cgtacgtcgg ccgccgtggt gacgacctcg ccgtccccgt
     1981 tgccaaccag tgccgtgccc gcgtcacgga actccgtgtc actcagggcc ttctcgaaca
     2041 cggtcacgac cggctccatg gtggaggtgt ggaacgctcc ggccaccggg agggtgacca
     2101 cccggactcc ctcgtgctcc tgctccgcca gggcggtgat ccgctcgtag cggcccgcgg
     2161 cgacgtactg gtggcacccg ttccagttgg cgatctccag tccggcgtcg tggatggtct
     2221 gctggatctc ctcatcagag gcggggtcac gccgggtcgg catgactgcc gccatcccgg
     2281 tacgaggctc ggtgcacgcc gccgacatgg ctgctcctcg cacacaagcc agggacacgg
     2341 cgtcggcgtc ggtgatgtac cccgccgccg ccgcggcagc cagctcccct accgagtggc
     2401 ccgcaaagag aatggcctcc gacgacgcac ccaggtaccg caccgtctgg cgcacggaca
     2461 gcaaggaggc tgcgacgatc acaggctggg caaacgctgt gtccttgagc ttctcgtcgt
     2521 cgcggctggc ctcgatcagg ttgacgccgc agcgcgacga ccactcctcc acctgacgtc
     2581 gtgccgtgcg ggagcggtcc atccacccgt caaggacccc cggcttctga gagccctgtc
     2641 ccggcgctgc tactatgtag tcgtatctca ctgaatatct ccagttcgtc tcatagtaaa
     2701 ccgatgtgct tactggccca ggctcgccgc cggcctctgc agcgccgtca cccgcgtgac
     2761 gctcatgagc gggcgcccct ccgaggtggc tgacgacccg gtgaactcgg ccacgtcgcc
     2821 gtcgcggtcc aggcactcga ccgtgtggac gacaagctcg cccgcacggg ggaggtcctg
     2881 caccgtcatc ccgcgcgccg cagcgaagac caccagggtc tcctggtctc cctcagcctc
     2941 gtgcttgagc aggatcccgc aggcctggag gaacgactcg atcatgaggc tcgcgggcag
     3001 ctgcgccgga cgtgaggagc cacccgccat cgcgtccagc tcgtgggagg agaccgcctt
     3061 gaccgcagtc agcctgcctg cctcgtagcc ggtcaccctg tcgacgtgca gcgcaggata
     3121 gccgtagggg agaagatgcc cgggatcggg aactccgtga gtcatcgccg cctccaggat
     3181 gccttgatgc tggccgtccg ccgccccgcg accgtgcagg aggtgctggt gagcaccagg
     3241 tcgccgtcgt gggtgacagt agtgtcaaag tcaactgtct gtcccgcgaa ggcgggtcgg
     3301 gagatacgta tggaccggac ctcgacgagc tcgacccctc ccagggcgcc gtccagggcg
     3361 gtctccagcg tctgctggac cccctccagc aggaagaccc cggggtagac ggtgagggag
     3421 gggaagtgcc ccgcgaggta ggggtcgtcg gtgcgcaccg tctttgcgga ccgcaccggc
     3481 tccccctcca cctcgcagcg gtccagggcg acaagaggga ggcgccgggc gtccgggcgt
     3541 actgacagtg tccccggtgt cccaggtgct ggtgtcccag gggccagcgt ctcaggggcg
     3601 gtcatggtca cagccccatc ccggcgccgc cgctgacagg aatcgacgcg cccgtgatgt
     3661 accccgcccc ctctgagacc aggtaggaca ccagggaggt cacctcctcc ggtctgccgg
     3721 ggcggcgcat cggcgtgccc gccatcagct ccttgtggcg cttggcggag acgttggccg
     3781 ccatgcccgt gtcgatcagg ccgggcagga tgacgttgac agtgaccccg aacctcccca
     3841 gctcccaggc cagcgacctc gcggccccca ccagcccgcc ctttgaggcg ccgtagttga
     3901 cctgcccggg ggacccccac atggcgacca cggagcccac gaacaccagg cgcccccact
     3961 ggttcatcat catgtcgccc agggtctcgc gggccacgtt gactgagccg agcaggttgg
     4021 tggtgatgac gtcgtccacg gcctcctggg ggagggagac gagcgggccg tcgtgcatga
     4081 ctcccgcact ggtcaccacg atgtctatcg tccccacgag gtcacgggcc tgggcgaccg
     4141 cgctgcggat ggagccgggg tccgtgacgt cgcaggccac gaacgagacg ccgtcaacga
     4201 cgtcctcggc agggtttcgt gccgtgccga ccacacggta cccctgagcg gccaggtccc
     4261 tggcgatggc ggcaccgagc cctcggctgg cgccagtgac gaacgcggac cgcgcgggcc
     4321 ctgctgactc ttccgtcatg agtgctgtcc ttcctgcagc ccgctgtgca ggctggcgag
     4381 ttcttctatg tacgcgatgc tgtcgtcggt ggcgtcgcct ccgccgacga cggcgttcca
     4441 gccagcgtcc ctggctccct ggacgttggc ggtgatgtcg tcgacgagca cgctgctgcc
     4501 tgccggaagg ctgctgacac gctccgcctc cctgaagatg ccgggctcgg gcttcctggc
     4561 gcccacctcg catgacacga cgaccgcgtc aaacaggtcg tgccagggca ggaactcctc
     4621 gaagcggccc tgccactcgg gcgggaggtt ggacatgaca gcgacgaagt ccccggcctg
     4681 cctgcgttcc tcaagaaagc gcagccactg gtggttgagc ctcctgtcgg accaccactc
     4741 gtcggccatc ccggagacgc tgccggtaat accgaactgc tgtgacagca ggccggagat
     4801 ctcgtcgagc cactcgcgct cggtcagcac gcctgagtcc aggacggcca tcccgtccgg
     4861 acacccgtgc ctgctggcca gagccgcgag gcaggtaccc agctgccggg gcgtgaaggg
     4921 cttgtcctgg cagtagcgtg cgaacccctc agacagtggg acggtgagca caccccggaa
     4981 gtccgtccat atggcgagag tcatcgcaac cactccacgg ccatctgcat ggaggagacg
     5041 gcagagccgt tctgctccac agacacctcg aaccggttgg cgcactcctg tgcggagacg
     5101 gtcgtgaccg cgtcgagctc ggcgaacccg ggaaacctcg cactcaccga ggtgacgaca
     5161 ggaaacccgt ttcctcccga cgccgagccc accgtcacct gccgaccggc ctcaatgagg
     5221 accatggcag ggtagtggtc gtagtcgtgg tcgaagaggc tggtgttggc gaagtccggt
     5281 gacagcctcg cgcgcgagcg tcccgggcca cagtccaggt cggagacgac gacgttgctc
     5341 atgtcctccc gtcccagcag cgagctcggg agcacgtgcg tgccggaaac ggcctggacc
     5401 tcgacggtgg acgtccgggg tggctgcgac ccgccccgct ggtacctgcg tatctcctcg
     5461 tacttggcgg cttggaggcc ctggacctcc atcctggtcc gggccagggg cgcgtcggca
     5521 gggtcgatga tctcctggga gaagaccagc tgccggggcg tgccgtcctt gcgggtggcc
     5581 aggacctcct gacgggtgcg gacccggggc ctgtcccgca gcccgttgag cctgggcagg
     5641 tcgaggatct ccaccatgac cgagttgaag aagaacgcgg tacccagtga gagccccgcg
     5701 taggcgtggc cggcgctcat tcccgcctgc ctgccgagct cggccaggaa caggacgtcc
     5761 gcccagtacg agtgcgggtg gtcccggtag tagggagacg gttctcccgg acccggtgcg
     5821 ctctcgtaca gcccgtcctc cagcctggtg ggagccgaga cgagcacctc ctgctcgtgc
     5881 tgacggtgga ccaggttccg gggggcgacc gttgctgcct gtggtgccat acgctgtgtc
     5941 cctccttgtg cgtgcggcag tgtcggtgac ggtgtcgcta gtggcgtctg tggcgtcgtc
     6001 tggctgggac ctcggctcac gagcggcaca tctggagcgt ggcggcagcc accacccctg
     6061 actcgtccat gctggtgagc aggacgcggt cgcctggccc cgcgcacgtc agggccgcgg
     6121 ccacctggac gccagaggtc ccggcgccgc agtcgcccac ggagcgtcgc acgtcaaggc
     6181 gacggccggt gacgtgctcc agcgagcgct cctcgctgag ctgccagacc gtgccggtgg
     6241 ctccggggac caccaggtcc acgtcctcgg gctggctgca cacgcggtcc accacccgcc
     6301 ggagagtctc ggaccgggca ccgtcggcgg agatagggca gaaggccacc tcgcagccgg
     6361 tgactgtcgc caccgggctc ggtgacgccc ccgcaggctc caggctgaac agggtggctg
     6421 actccccgat caccacctcg ctgtcgatcc tgccgctcag gcgcgttccc caggctcgct
     6481 ggggggagag ctcctcgaca ctgccgacca gcatccggga gcagtgcccc agggacatga
     6541 gccgtgaggc gtagcgcagg gcggacaggg cgctgacggt gccgttggcc actgtggcgt
     6601 tcggtccgtg gaagtcgttc cagatcgcga cctggctcgc gcagcagttc atggtgctgt
     6661 tcgggaagac cttggggttg atcaggtccg caccacgccc gcggatcacg tcgagggtga
     6721 ggtcgacgtt gctcctcagg ctgcctgcgc tcgtccccag ggagattcct gtctcgcgcc
     6781 aggagtcggc agcgatgtca cgccgctcct ccatctggcg cctcagcagg acgctggccg
     6841 cgatgcccag ccgtgtcata cggtcaaggc tcctcgtgcc gcgccggggg acatagtcct
     6901 caatgcggaa gtccgggact gcggccacgc gtcccgggac cccctcgtac acctccgggt
     6961 cgggaagcgc gccgagctct gacggcgcac ggctgcccag gggcctgtcg atggcctcgg
     7021 tgagcgtgtc catgctgttt ccggccgccg tcaggaccgc gcactcgctg atctgcatct
     7081 caggggctcc catgcgtcac acctccttgt ccgccgagtc cttgtggaac tctgagaaga
     7141 cggttatcgc gttgtttccg ccgaaggcga atccgtggtt gagcgcggtc cgcacccgtg
     7201 ccgggcgcga gctgcccgcg acgaagtcga ttccctcgcc gagctcgggg tcgaccgtcc
     7261 tcaggttggt cgtgggcggc agcttctgga tctggatggc ctggcagcag gcgatcgcgc
     7321 cgaacccgct ggcagcaccc atggtgtgcc cgagcatgga cttgatggag ctgaccgggg
     7381 ggtggtcgtc cccgtagacc atgcgggtgg ccgagacctc ggtgacgtcg ttggtcggag
     7441 tgcccgtgcc gtgggcgcag acgtagtcga cgtcgtccgg ggtgatgttg gcctgcgcga
     7501 gggtgcgcct gatgcactcc gagatgctcg tggcatccgg gtgcacgatg tggtgggcgt
     7561 cgcagttgag accgtagccc agtacctcgg cgtagatcgg tgcttctcgc cgcagggcgt
     7621 gactcagctc ctccaggacc agtaccgcgc cgccctcagc cgtcacgatg cccgagcggt
     7681 ctgcgtcaaa gggctgcggc agctccttgg cgatagcgcc caggctcatg aacccggcgt
     7741 gcgtcgtgcg gttgacggag tcggcacccc cggccagcat gacgtccgca ctaccggtcg
     7801 caatactgtc ggcggctgag ccgatggcgt agttggcggc cgagcaggcg gtcggcaggc
     7861 acatgacgtc gccctcgacc tggagctccg tggccacggc ggcagcaatg ttgccagccg
     7921 ggatctggcg cacgacggtt ggtgccatgc gccctggacc acgttcgtgc cactggacgg
     7981 tcagctcgtc cagggcctgc gactcgccgt tggtcgtccc gatgacgacc cctgtgctca
     8041 gccttgtctc ctggtcgagg tcgagtcctg cgtcgcgtac cgccagcctg gcggccgcag
     8101 cggccagctg tgcggtacgt ccccagcgac gggggttgat gttctcgaca acatctgcgg
     8161 ggtcgaagtt tttcacctca cccgccagcg tcgcctggaa gtcgctggtg tcgaaagact
     8221 ccacggggga gatgccgctc tctcccgcga acagcagggc acggaactcc tccgccccga
     8281 taccgatgct gctcaccggg ccgagcccgg tgacgacgac tcgtctgctg cggctcattt
     8341 gtcgctaccc tgctcggcct gcagccggac cgtcttcgcc acgagcaggt cgagcgtgcg
     8401 aaggtcagcc gcctcctcgc gcggcacgcg gatgccgagg gtcttgttga tctgtgccag
     8461 gacctggatc agacccaggg agtcggcgcc gtactcgtcg atgaggtccg cgtgcggatc
     8521 cagctcgtcg acctcgatat cgagctcgag ctcctcgacc acgatctcgc tgatggcgtc
     8581 gcggatctca ttgctggtgc tgcttgtcat ttctggttac tccatttctt tcacatggtt
     8641 tgtgggcata aagacacgag tgcccactgg ctggggacca gccgctggag acgggcagtg
     8701 cccgcctcac gcctcaatgt cgagctgaga catgcgggta agccgtgccc gtgccgcgtc
     8761 cgggttctcc aggagcgcgt acctggtgtc tcgctcggcg atgctctcgg gggtcatggc
     8821 tgcgccgtcc ctggcgtgcc cgagcgcggt gtcgaacacc ttgtcaatgc tggcgcgacg
     8881 catcttgtcg tagagctgtg ccagaggggt gcctgagtcg acgggctcga tgtcgcccat
     8941 gatgaacttg ctggcgcaca ccgcgtccag catgcctgcg ttcatgttct gggcaccggt
     9001 aggtgccatg acgtgggcag cgtccccggt taggtagcag cgccccttgg cgaactggtc
     9061 cacgacggcg aaccgggcca caaagtttgc cgttgtgatc agctgcgtga catgcctgtc
     9121 ggggttggac ggaccccgct ggtccagcag ggactccagg tactgcgtcg ataccgcgtc
     9181 cggtgccggg gtccctgtga cggcaggata ggccaggcgg taggtcgtct ggtcgtgggg
     9241 agccacggca atggccccgt actgggacca ccagtagaat gcctcgtcgc gaggaagccc
     9301 ctcggccctg acgtcggcga tctggaactc gacggtgtcg cgcaggtcct ccatgcgcag
     9361 accgagcttc tcccggccca ggctcttggc gccgtcggcg accacgcagt gcttagcccg
     9421 ctcctggcgc actgttccgt caaaggagcg gatggtcagg aggactgagt cgtcgtcctg
     9481 cgtgaggtcg gtgacctcgg cgtcctcaat gactccgccc cgggccgtga agacctcggt
     9541 cagggctgtc tcgacgttgt gctgggagat gcccagcgcg tagtcgaacc gtgtgccttc
     9601 cagcagccgg gtgctgagcg tgccgatttg gcggccgtcg gagtagtagg agaggctgtt
     9661 gatgacgtca ccaagaggcc ggagcctctc cgcgatgccg aaagggtgca ggacctccag
     9721 cgccttgggc cacaggaccg ttgcgcggga ctggctggtc agtagggggt gctttcggat
     9781 gatcctgacg ctctgtccgc gctcgaccag gcagcacccg gctgtgaggc cagagggtcc
     9841 cccaccgatg acaagtgtgt ctgagttcaa ttctctttct ccgtgataat atatttacag
     9901 aggttacatt ggtgactact gtgtgggacg tactggaact tcatccgagt ggccccgata
     9961 aatctgttgc tcttaaattg ttgttgaaac cttgccctgg cccttggcgc cggggactct
    10021 cgttgccggt gcgagatgcc tcggcttctg ctccgtgggg gcgccttaac ggggacaggt
    10081 gtcgtaggtg ctggcaggca gccctgtggg tgatgaggcg cgttgtcggg tggggtgtcg
    10141 ctgtgggggc ggcgcctctt ccgtcaatgg agaaggtgtg catgttgtgg cgggtgcggg
    10201 gcggggtggt tccgctgctc ctgccggtgt tacgggaccc ctgagggtag ggtggcggct
    10261 tccgacgtgg gggctcggtg ctacttatac gggaggtggt tgtctgctgc gcccgggtga
    10321 cgcgtggtga cggagaggct gcagagaggc cgaggcctgg tgcttgggtg ggtggaggcc
    10381 ggtgctggtg cggttgcacg ggtctggccg agtggtgctg tgtgtctgtg gtagggctca
    10441 gaggcctcct ggtgcaggtt ggcttgactg gtgacggggg ctgacagcgc ggtcggggtg
    10501 cgtcggcagg accaggtggc tgcgttcagg aaggggagag ggcgcgctca tagtgaatag
    10561 tggctcgggg gtctagcgtg cctgcttgtc acctgggttg gcatcaatgc gtgcgccaaa
    10621 cataatgtag aggatggcga cggtgatgaa aattcccagt acccagataa atccggcttg
    10681 catggtggcg gcgacggaga acacggcccc cagggctgcc actcccatgg caacggtcgt
    10741 ctgctgaacc gtggtaatca gcccgctccc gaggccgacg cgctgcggcg ggatggctgc
    10801 cagcacggtc cggtagatga taggaacgaa gaggccgttg ccgagcccga tgaggaacat
    10861 cccgaggagg aggtggtaga agtgcatgtg gggccagtac aggtaggcgg gcaggatgac
    10921 ggcggcggcg
//
